CN1875960A - A nanoemulsion medicine of eugenol and preparation method thereof - Google Patents
A nanoemulsion medicine of eugenol and preparation method thereof Download PDFInfo
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- CN1875960A CN1875960A CN 200610043054 CN200610043054A CN1875960A CN 1875960 A CN1875960 A CN 1875960A CN 200610043054 CN200610043054 CN 200610043054 CN 200610043054 A CN200610043054 A CN 200610043054A CN 1875960 A CN1875960 A CN 1875960A
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Abstract
The invention discloses the eugenic nanometer drug, comprising isopropyl microstate, croton oil, vitamin E oil, tuwen-80, spans-80 and eugenic. The drug has the advantages of improving the antibiosis, anti-inflammation, disinfection, analgesic, and ant parasitic. So the drug is green, safe and highly effective ant parasitic agent.
Description
Technical field
The invention belongs to field of veterinary, relate to a kind of medicine, be specifically related to a kind of nanoemulsion medicine of eugenol and preparation method thereof with anti-inflammation, parasite killing anthelmintic, antipyretic-antalgic, anti-parazoon.
Background technology
Eugenol (Eugenol) is a kind of faint yellow or colourless clear and bright buttery dulcet chemical compound, is a kind of good plant source medicine.Eugenol has pharmacological action widely, mainly contains the effect of anti-inflammation, anti-corrosive fresh-keeping, parasite killing anthelmintic, antipyretic-antalgic, anti-parazoon.Because eugenol belongs to volatile oil, the utmost point is insoluble in water, and poor stability is easy to scatter and disappear in the production of conventional formulation and storage, influence drug effect, is that present technology has a technical barrier to be solved.
Summary of the invention
The objective of the invention is defective and deficiency at above-mentioned prior art, provide a kind of veterinary clinic to be used for the nanoemulsion medicine of eugenol that contains of anti-inflammation, parasite killing anthelmintic, antipyretic-antalgic, anti-parazoon, the present invention is logical to make nano-emulsion with eugenol, strengthened the effectiveness of eugenol greatly, simultaneously also solve the eugenol utmost point and be insoluble in water, the shortcoming of poor stability.
Because nano-emulsion substrate belongs to the nanoscale emulsion, can make medicament solubilization in the nano-emulsion system, improve its stability, simultaneously also can improve percutaneous permeability, the raising utilization ratio of drug of maximizing, so the present invention is solubilized into nano-emulsion substrate with eugenol, make the animal skin medicine for external use, be intended to by this invention to play treatment and preventive effect animal parasitosis for novel anti-parasite medicine is provided on the veterinary clinic.
The purpose that realizes foregoing invention is that scheme is a kind of nanoemulsion medicine of eugenol, and each amounts of components is for all to have effect preferably in following weight percentage ranges:
Isopropyl myristate: 0.77%～15.38%
Jojoba oil: 0.15%～3.08%
Vitamin E oil: 0.08%～1.54%
Tween 80: 5.83%～37.50%
Arlacel-80: 1.17%～7.50%
Deionized water: 25.00%～91.90%
Eugenol: 0.10%～10.00%
The formula optimization weight percentage ranges of preparation medicine of the present invention is:
Isopropyl myristate 0.77%～11.26%, Jojoba oil 0.15%～2.18%, vitamin E oil 0.08%～1.38%, tween 80 5.83%～27.80%, Arlacel-80 1.17%～5.50%, deionized water 50.00%～82.00%, eugenol 0.1%～5.00%.
The prescription optimum weight percentage of preparation medicine of the present invention is:
Isopropyl myristate 10.00%, Jojoba oil 2.00%, vitamin E oil 1.00%, tween 80 16.25%, Arlacel-80 3.25%, deionized water 57.50%, eugenol 10.00%.
Another object of the present invention provides the preparation method of nanoemulsion medicine of eugenol, may further comprise the steps:
1) take by weighing each drug component isopropyl myristate, Jojoba oil, vitamin E oil, tween 80, Arlacel-80, deionized water, eugenol, standby;
2) under 13 ℃～32 ℃ room temperature conditions, isopropyl myristate, Jojoba oil, vitamin E oil, tween 80 and the Arlacel-80 with described weight places on the constant temperature blender with magnetic force earlier, stirs 10min, mix homogeneously with 200～400rpm;
3) in mixture, slowly drip deionized water, stir while dripping; System viscosity is less during beginning, increase with rate of water added, it is sticky that system can become, this moment, liquid crystalline phase or oil-in-water type nano-emulsion may appear in system, continue to drip and constantly stir, when the water yield reaches a certain amount of, system can be thinning suddenly, what produced this moment promptly is O/W type nano-emulsion, deionized water is added to q.s, is prepared into clear O/W type nano-emulsion, again eugenol is added in the nano-emulsion at last, continuation is stirred with 200～400rpm in constant temperature blender with magnetic force, muddy phenomenon can occur in the process that stirs, continue to keep 200～400rpm and stir, keep 10～20min, system can become clear suddenly again, flaxen liquid promptly is prepared into nanoemulsion medicine of eugenol.
The present invention is solubilized into nano-emulsion substrate with eugenol to be prepared into nanoemulsion medicine of eugenol, and can be diluted to the fragrant phenol nano-emulsion liniment and the eugenol nano emulsion body lotion of variable concentrations according to actual needs with deionized water.
The present invention detects through transmission electron microscope, and 10% eugenol nano emulsion outward appearance is faint yellow clear liquid, and the emulsion droplet particle size distribution is between 10～80nm, and mean diameter is about 50nm.Thermodynamic stability of the present invention is good, and excellent storage stability is put not stratifiedly for a long time, even in centrifugal acceleration test, the centrifugal 30min of 10000rpm layering can not take place yet; Antiseptic property of the present invention is better, does not need other adding preservative agent, so have the better anticorrosion and anti-ability of going mouldy than the commercial like product, time shelf-life is long.
The present invention has following beneficial effect in sum:
1. the invention belongs to the nanoscale emulsion and have better percutaneous permeability, energy is the kill animals endoparasite and ectoparasite fast, improves the curative effect of eugenol nano emulsion;
2. eugenol nano emulsion belongs to the plant source medicine, can not cause food, meat drug residue and environmental poisonous substance danger and cause of disease drug resistance, and the present invention is a kind of good green, safety, parasiticide nano-grade medicine efficiently;
3. thermodynamic stability of the present invention is good, excellent storage stability, put for a long time not stratified, even can layering in centrifugal acceleration test yet, time shelf-life is long;
4. the invention belongs to nano-grade medicine, the outward appearance clear, emulsion droplet particle diameter average out to 50nm, dispersion is good, and available deionized water is diluted to 0.5% eugenol nano emulsion body lotion and 1% eugenol nano emulsion liniment;
5. it is simple to prepare newborn substrate technology by above method, is convenient to operation, and the needed raw material moderate cost, can be accepted by consumers in general.
The specific embodiment
Below further sets forth the beneficial effect of medicine of the present invention by testing example, these are tested the routine pharmacodynamics test of medicine of the present invention and the clinical observation on the therapeutic effect of having comprised and test.
Test example 1 medicine of the present invention exsomatizes to the rabbit of natural infection acaricide and kills the demodicid mite test
Test material:
Demodicid mite Lingshui Spring solution (Hunan China Shaoshan Shiyuan Veterinary Pharmaceutical Factory is killed in anthology invention medicine eugenol emulsion, 1% eugenol solution (solution substrate is that 1: 1: 8 Tween80, ethanol and distilled water is formulated), 600 times of dilutions, lot number 20050503), abamectin injection (Harbin is green to reach lively thing pharmaceutcal corporation, Ltd, lot number 20050904).
Test method:
Scrape the crust and the epidermis affected part scurf that cover with blood crusts in the rabbit auditory meatus of taking from right infection acarid, and place in the glass dish with the tweezers taking-up, the plate edge is coated with a small amount of acarid repellant, heats a little, is for experiment with choosing the active strong acarid of worm pin picking.Carrying out the polypide evaluation under anatomical lens, is Long Circle for the examination demodicid mite, long 0.5～0.9mm, and the polypide front end has cone shape mouthpart, the outside of belly has 4 pairs of foots, and two pairs of foots of front are thick, and two pairs of foots of back are elongated, outstanding health edge, there are two big projections, hairiness on the projection in male worm outside of belly rear portion; The rabbit acaricide is circular, and canescence is about 0.2～0.5mm, the back protuberance, and the outside of belly is flat, and there are many thin bands, scale and bristle in the health back side, and the outside of belly has 4 pairs of stubby lower limbs, and anus is at the polypide back side, and is nearer apart from the polypide trailing edge.Be defined as psoroptes cuniculi and rabbit acaricide according to the polypide morphological characteristic.
With separating pin picking acarid on the spill microscope slide, drip for two of reagents, put 8 of the acarids of selecting in the medicinal liquid and add coverslip, under inverted microscope, continue to observe 24h, observe 1 time every 5min, each observation 2min that continues observes until all death of 8 acarids, record acarid death time for every group.
Criterion:
The polypide podomere is motionless, and the podomere of slightly heating vibration is judged to be paralysis; The polypide podomere is motionless, and the nothing of slightly heating recovery or polypide shrinkage, variable color, distortion are judged to be death.During off-test, will test the acarid that exsomatizes and place on 37 ℃ of water-baths heating a little to observe polypide recovery situation; Establish avilamycin, demodicid mite spirit extremely, 1% eugenol solution simultaneously for contrasting, test is at room temperature carried out.
Result of the test shows: different medicinal liquids are killed acarid result demonstration after handling 24h, 10% eugenol emulsion is killed demodicid mite and is only needed 1min, 5%, 1%, 0.5% eugenol emulsion is killed demodicid mite and is respectively 3～4min, 8～11min, 20～22min, miticidal effect kills demodicid mite Lingshui Spring solution apparently higher than 1% eugenol solution and 600 times of dilutions, and suitable with the avilamycin miticidal effect, the results are shown in Table 1.
The table 1 miticidal effect comparative result that exsomatizes
| Medicine and concentration | Acarid number (only) | Death toll (only) | The polypide death time (min) | Recovery |
| Mite Lingshui Spring solution abamectin injection 1% eugenol solution is killed in 600 times of dilutions of 10% eugenol emulsion, 5% eugenol emulsion, 1% eugenol emulsion, 0.5% eugenol breast | 8 8 8 8 8 8 8 | 8 8 8 8 8 8 8 | 1 3～4 8～11 20～22 4～6 2～3 50～54 | - - - + + - - |
Annotate :+expression has the acarid survival;-expression acarid all kills does not have recovery
Test example 2 medicines of the present invention carry out the rabbit body to the rabbit of natural infection acaricide and kill the demodicid mite test
Test material:
To test 18 of rabbits and be divided into A at random, B, C, totally 6 groups of D, E, F, every group of 3 sick rabbits, male or female.
Test method:
A, the B group is respectively 1% eugenol emulsion and demodicid mite Lingshui Spring solution is killed in 300 times of dilutions, A, the B group is all dipped in for the reagent thing with absorbent cotton, is coated with repeatedly and puts disease rabbit affected part treatment on the skin, and continuous once a day two days, repaste was put on the skin once behind the 7d; C, D group is respectively 0.5% eugenol emulsion and demodicid mite Lingshui Spring solution is killed in 600 times of dilutions, with the whole whole body dipping of sick rabbit 3min, and is coated with the absorbent cotton dip in liquid medicine and puts head and auricle affected part on the skin, proposes disease rabbit and grouping and puts into no demodicid mite cage raising, and dipping is once again for interval 7d; The F group is abamectin injection, a subcutaneous injection 0.3mg/kg, and 7d injects once more at interval; The G group is blank newborn substrate matched group.Test back observed and recorded every day is tested disease rabbit drinking-water, appetite, mental status and each clinical symptoms, and after each administration 5d, every rabbit is glued the scurf of getting diseased region with the adhesive tape method at affected part, take a sample to check acarid (and demodicid mite ovum), under anatomic microscope, observe the acarid survival condition, counting demodicid mite alive and dead demodicid mite draw average demodicid mite number alive and dead demodicid mite number, and calculate average acaricidal rate.Test is at room temperature carried out.
Result of the test
After testing twice administration, A, C, E organizes 5d and observes disease rabbit pruritus symptom and obviously alleviate, and serious diseased region skin crust begins to come off, and diseased region sampling microscopy counting is seen demodicid mite alive, all crusts of each diseased region of 14d all come off, it is smooth red tender that skin becomes, and what have grows virgin wool, and affected skin sampling microscopy counting is not seen demodicid mite alive and demodicid mite ovum; B, the C group found that through rabbit body miticidal effect viewing test, the sick rabbit pruritus of 5d obviously alleviates, but pars affecta skin still has incrustation, and crust is scraped off; diseased region sampling microscopy counting is seen demodicid mite alive; the sick rabbit clinical symptoms of 14d disappears substantially, and the affected part crust partly comes off, and skin is light red; but serious diseased region still has crust, and diseased region sampling microscopy still has the acarid survival; E organizes after the medication first 5d and scratches where it itches and alleviate, and the affected part crust begins to come off, and diseased region sampling microscopy counting is seen demodicid mite alive, the complete obiteration of the sick rabbit clinical symptoms of 14d, and decrustation, skin is red tender, and what have grows virgin wool, and diseased region sampling microscopy still has demodicid mite alive to exist; F organizes disease rabbit clinical symptoms does not have any improvement.The results are shown in Table 2.
Table 2 rabbit body miticidal effect viewing test result
| Group | Medicine and concentration | Medication for the first time | Medication for the second time | ||||
| Demodicid mite alive/only | Dead demodicid mite/only | Acaricidal rate/% | Demodicid mite alive/only | Kill demodicid mite/only | Acaricidal rate/% | ||
| A B C D E F | 300 times of dilutions of 1% eugenol emulsion are killed 600 times of dilutions of demodicid mite Lingshui Spring solution 0.5% eugenol emulsion and are killed demodicid mite Lingshui Spring solution abamectin injection matched group | 18 22 16 27 13 73 | 59 47 53 39 53 5 | 76.6 68.1 76.8 59.1 80.3 - | 4 11 5 18 6 87 | 68 54 63 45 65 8 | 94.4 81.8 92.6 71.4 91.5 - |
Annotate :-expression is not calculated
Kill rabbit demodicid mite and rabbit body through exsomatizing through the present invention and kill demodicid mite test discovery, no matter the present invention is to kill in the demodicid mite test at the stripped psoroptes cuniculi and the test of rabbit acaricide or rabbit body of killing, therapeutic effect is all apparently higher than commercially available demodicid mite spirit extremely, and suitable, but also has the entozoal effect of good curing animal skins with the abamectin injection miticidal effect.
Come further to set forth medicine of the present invention and preparation method thereof by the following examples.
Embodiment 1:
1) take by weighing each drug component eugenol 0.10g, isopropyl myristate 0.77g, Jojoba oil 0.15g, vitamin E oil 0.08g, tween 80 5.83g, Arlacel-80 1.17g, deionized water 91.90g, standby;
2) under 25 ℃ of room temperature conditions, isopropyl myristate, Jojoba oil, vitamin E oil, tween 80 and the Arlacel-80 with described weight places on the constant temperature blender with magnetic force earlier, stirs 10min, mix homogeneously with 300rpm;
3) in mixture, slowly drip deionized water, stir while dripping.System viscosity is less during beginning, increase with rate of water added, it is sticky that system can become, this moment, liquid crystalline phase or oil-in-water type nano-emulsion may appear in system, continue to drip and constantly stir, when the water yield reaches 24.00g, system can be thinning suddenly, what produced this moment promptly is O/W type nano-emulsion, deionized water is added to 91.90g, is prepared into clear O/W type nano-emulsion, in the last eugenol adding nano-emulsion with described weight again, continuation is stirred with 300rpm in constant temperature blender with magnetic force, muddy phenomenon can occur in the process that stirs, continue to keep 300rpm and stir, keep 20min, system can become clear suddenly again, flaxen liquid promptly gets 0.5% nanoemulsion medicine of eugenol, 100.00 grams of the present invention.
Embodiment 2:
1) take by weighing each drug component isopropyl myristate 4.62g, Jojoba oil 0.92g, vitamin E oil 0.46g, tween 80 11.67g, Arlacel-80 2.33g, deionized water 85.00g, eugenol 5.00g, standby;
2) under 25 ℃ of room temperature conditions, isopropyl myristate, Jojoba oil, vitamin E oil, tween 80 and the Arlacel-80 with described weight places on the constant temperature blender with magnetic force earlier, stirs 10min, mix homogeneously with 300rpm;
3) in mixture, slowly drip deionized water, stir while dripping.System viscosity is less during beginning, increase with rate of water added, it is sticky that system can become, this moment, liquid crystalline phase or oil-in-water type nano-emulsion may appear in system, continue to drip and constantly stir, when the water yield reaches 21.00g, system can be thinning suddenly, what produced this moment promptly is O/W type nano-emulsion, deionized water is added to 85.00g, is prepared into clear O/W type nano-emulsion, in the last eugenol adding nano-emulsion with described weight again, continuation is stirred with 300rpm in constant temperature blender with magnetic force, muddy phenomenon can occur in the process that stirs, continue to keep 300rpm and stir, keep 20min, system can become clear suddenly again, flaxen liquid promptly obtains 5% nanoemulsion medicine of eugenol, 100.00 grams of the present invention.
Embodiment 3:
1) take by weighing each drug component isopropyl myristate 15.38g, Jojoba oil 3.08g, vitamin E oil 1.54g, tween 80 37.50g, Arlacel-80 7.50g, ionized water 25.00g, eugenol 10.00g, standby;
2) under 25 ℃ of room temperature conditions, isopropyl myristate, Jojoba oil, vitamin E oil, tween 80 and the Arlacel-80 with described weight places on the constant temperature blender with magnetic force earlier, stirs 10min, mix homogeneously with 300rpm;
3) in mixture, slowly drip deionized water, stir while dripping.System viscosity is less during beginning, increase with rate of water added, it is sticky that system can become, this moment, liquid crystalline phase or oil-in-water type nano-emulsion may appear in system, continue to drip and constantly stir, when the water yield reaches 12.00g, system can be thinning suddenly, what produced this moment promptly is O/W type nano-emulsion, deionized water is added to 25.00g, is prepared into clear O/W type nano-emulsion, in the last eugenol adding nano-emulsion with described weight again, continuation is stirred with 300rpm in constant temperature blender with magnetic force, muddy phenomenon can occur in the process that stirs, continue to keep 300rpm and stir, keep 20min, system can become clear suddenly again, flaxen liquid promptly gets 10% nanoemulsion medicine of eugenol, 100.00 grams of the present invention.
Claims (6)
1. nanoemulsion medicine of eugenol, form by following raw materials by weight percentage:
Isopropyl myristate 0.77%～15.38%, Jojoba oil 0.15%～3.08%, vitamin E oil 0.08%～1.54%, tween 80 5.83%～37.50%, Arlacel-80 1.17%～7.50%, deionized water 25.00%～91.90%, eugenol 0.10%～10.00%.
2. ask according to right and want 1 described nanoemulsion medicine of eugenol, wherein the mass percent of each raw material is:
Isopropyl myristate 0.77%～11.26%, Jojoba oil 0.15%～2.18%, vitamin E oil 0.08%～1.38%, tween 80 5.83%～27.80%, Arlacel-80 1.17%～5.50%, deionized water 50.00%～82.00%, eugenol 0.10%～5.00%.
3. ask according to right and want 1 described nanoemulsion medicine of eugenol, wherein the percentage by weight of each raw material is:
Isopropyl myristate 10.00%, Jojoba oil 2.00%, vitamin E oil 1.00%, tween 80 16.25%, Arlacel-80 3.25%, deionized water 57.50%, eugenol 10.00%.
4. according to the described a kind of nanoemulsion medicine of eugenol of claim 1～3, it is characterized in that: the emulsion droplets particle diameter of described nanoemulsion medicine is 10～100nm.
5. according to the described a kind of nanoemulsion medicine of eugenol of claim 1～3, it is characterized in that described nanoemulsion medicine can be diluted to the liniment and the body lotion of variable concentrations with deionized water.
6. the preparation method of the described nanoemulsion medicine of eugenol of claim 1 is characterized in that, may further comprise the steps:
1) take by weighing each drug component isopropyl myristate, Jojoba oil, vitamin E oil, tween 80, Arlacel-80, deionized water, eugenol, standby;
2) under 13 ℃～32 ℃ room temperature conditions, isopropyl myristate, Jojoba oil, vitamin E oil, tween 80 and the Arlacel-80 with described weight places on the constant temperature blender with magnetic force earlier, stirs 10min, mix homogeneously with 200～400rpm;
3) in mixture, slowly drip deionized water, stir while dripping; System viscosity is less during beginning, increase with rate of water added, it is sticky that system can become, this moment, liquid crystalline phase or oil-in-water type nano-emulsion may appear in system, continue to drip and constantly stir, when the water yield reaches a certain amount of, system can be thinning suddenly, what produced this moment promptly is O/W type nano-emulsion, deionized water is added to q.s, is prepared into clear O/W type nano-emulsion, again eugenol is added in the nano-emulsion at last, continuation is stirred with 200～400rpm in constant temperature blender with magnetic force, muddy phenomenon can occur in the process that stirs, continue to keep 200～400rpm and stir, keep 10～20min, system can become clear suddenly again, flaxen liquid promptly is prepared into nanoemulsion medicine of eugenol.
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| CN100488359C (en) * | 2007-04-26 | 2009-05-20 | 江苏省农业科学院 | Eugenol micro-emulsion |
| CN102274177A (en) * | 2011-08-24 | 2011-12-14 | 西北农林科技大学 | Compound thymol nanometer medicament and preparation method thereof |
| CN102283850A (en) * | 2011-08-25 | 2011-12-21 | 西北农林科技大学 | Oil-in-water type compound ketoconazole nano-medicament and preparation method thereof |
| CN103140138A (en) * | 2010-09-09 | 2013-06-05 | 赛达国际公司 | Fungicidal bactericidal and/or antioxidant treatment of foodstuffs via application of formulations comprising an essential oil and a dialkylene glycol |
| CN103975921A (en) * | 2014-04-25 | 2014-08-13 | 浙江大学 | Pre-harvest sprouting inhibitor and method for inhibiting pre-harvest sprouting of rice |
| CN104288131A (en) * | 2014-09-24 | 2015-01-21 | 浙江省淡水水产研究所 | Application of eugenol for killing ichthyophthirius multifiliis |
| CN107019138A (en) * | 2017-06-15 | 2017-08-08 | 佛山科学技术学院 | A kind of eugenol combination liquid and preparation method and application |
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| CN114831143A (en) * | 2022-03-22 | 2022-08-02 | 河南抱一堂医药科技有限公司 | Biological organic anthelmintic and preparation method and application thereof |
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| CN1079392A (en) * | 1992-06-03 | 1993-12-15 | 山东省立医院 | Method for preparing yulelu emulsion |
| JPH08268837A (en) * | 1995-03-31 | 1996-10-15 | Lion Corp | Emulsion-type composition |
| CN1368337A (en) * | 2001-02-05 | 2002-09-11 | 杨孟君 | Nano medicine 'Wanbao' and its preparing process |
| US20050266085A1 (en) * | 2004-05-28 | 2005-12-01 | Warner Kevin S | Gelled emulsion and microemulsion formulations for dermal drug delivery |
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| CN100488359C (en) * | 2007-04-26 | 2009-05-20 | 江苏省农业科学院 | Eugenol micro-emulsion |
| CN103140138A (en) * | 2010-09-09 | 2013-06-05 | 赛达国际公司 | Fungicidal bactericidal and/or antioxidant treatment of foodstuffs via application of formulations comprising an essential oil and a dialkylene glycol |
| CN102274177A (en) * | 2011-08-24 | 2011-12-14 | 西北农林科技大学 | Compound thymol nanometer medicament and preparation method thereof |
| CN102283850A (en) * | 2011-08-25 | 2011-12-21 | 西北农林科技大学 | Oil-in-water type compound ketoconazole nano-medicament and preparation method thereof |
| CN103975921A (en) * | 2014-04-25 | 2014-08-13 | 浙江大学 | Pre-harvest sprouting inhibitor and method for inhibiting pre-harvest sprouting of rice |
| CN103975921B (en) * | 2014-04-25 | 2016-04-13 | 浙江大学 | A kind of method that fringe is sprouted inhibitor and suppressed Rice Panicle to be sprouted |
| CN104288131A (en) * | 2014-09-24 | 2015-01-21 | 浙江省淡水水产研究所 | Application of eugenol for killing ichthyophthirius multifiliis |
| CN107019138A (en) * | 2017-06-15 | 2017-08-08 | 佛山科学技术学院 | A kind of eugenol combination liquid and preparation method and application |
| WO2021224943A1 (en) * | 2020-05-05 | 2021-11-11 | Indian Institute Of Technology Bombay | Nutraceutical composition and method of production thereof |
| CN112972437A (en) * | 2021-02-19 | 2021-06-18 | 沈阳伟嘉生物技术有限公司 | Long-acting external preparation containing iodonitrophenol and preparation method and application thereof |
| CN114831143A (en) * | 2022-03-22 | 2022-08-02 | 河南抱一堂医药科技有限公司 | Biological organic anthelmintic and preparation method and application thereof |
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