CN1857717A - Anticancer peptide extract of blood clam and its preparing method - Google Patents
Anticancer peptide extract of blood clam and its preparing method Download PDFInfo
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- CN1857717A CN1857717A CNA2005100437974A CN200510043797A CN1857717A CN 1857717 A CN1857717 A CN 1857717A CN A2005100437974 A CNA2005100437974 A CN A2005100437974A CN 200510043797 A CN200510043797 A CN 200510043797A CN 1857717 A CN1857717 A CN 1857717A
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Abstract
The present invention relates to anticancer blood clam peptide with high anticancer activity and its preparation process and belongs to the field of medicine technology. The anticancer blood clam peptide is prepared with blood clam as one kind of marine life and through serial technological process of extraction, precipitation, chromatographic separation, desalting, etc. The preparation process includes biological extract, staged ammonium sulfate precipitation, Superdex75 gel filtering and chromatographic separation, and Sephade G-25 column desalting. The screened out peptide molecules below 3000 Dal have optimal pharmaceutical effect and economic utility. The injection with the matter of the present invention has broad spectrum anticancer curative effect and low toxic side effect.
Description
Technical field
The present invention relates to a kind ofly handle and prepare a class and have Scapharca subcrenata antineoplaston extract of high anti-cancer activity and preparation method thereof through a series of technical measures such as extraction, precipitation, chromatography, desalination from the marine organisms Scapharca subcrenata, it belongs to medical technical field.
Background technology
From marine organisms, especially from Scapharca subcrenata, can prepare extract with active anticancer through technical finesse.By retrieval, Chinese patent application numbers 98110478.9 discloses " marine organism extract that a kind of energy is anticancer " technology, and content relates to be pulverized low temperature hydrolysis through being soaked in water and obtain glycoprotein isoreactivity material from Scapharca subcrenata or Scapharca broughtonii; Chinese patent application number 03139763.8 discloses a kind of " purposes and the extracting method of polypeptide protein class active substance in the Scapharca broughtonii ", the disclosed extracting method of this patent application be directly with extract after ion-exchange chromatography, desalination, separate through gel filtration chromatography again; Chinese patent application number 03157458.0 discloses one " polypeptide protein class active substance in the Scapharca subcrenata and its production and use ", the disclosed extracting method of this patent application be directly with extract after ion-exchange chromatography, desalination, separate intercepting molecular weight 3-150kDa composition through gel filtration chromatography again.
Through the relative analysis background technology as can be seen, there are some shortcoming and defect like this in prior art:
1., the extract of prior art is glycoprotein or polypeptide protein, i.e. protide composition, and non-peptide compound.
2., the extracting method of prior art is conventional preparation method, conventional preparation method technology backwardness, inefficiency, precision are not high, can't carry out the tiny differential of molecular weight.
3., the molecular weight of prior art is greater than 3000Dal, the albumen pharmacological effect and the economical and technical benefit of macromolecule are relatively poor, are unfavorable for being prepared into injection for clinical use.
4., prior art adopts centrifugal extraction under the generic condition, centrifugal leaching process is difficult to guarantee the activity of extract, effect is relatively poor.
5., the extraction step of prior art is comparatively extensive, contains plurality of impurities in the extract, should not be directly used in the high field of purity requirement.
6., prior art adopts gel permeation chromatography step after the first desalination more, makes the impurity in the eluent comprise that salt remains in the final extract.
Summary of the invention
Purpose of the present invention is exactly in order to overcome and avoid the shortcoming and defect of prior art, a kind ofly handles and prepares a class and have Scapharca subcrenata antineoplaston extract of high anti-cancer activity and preparation method thereof through a series of technical measures such as extraction, precipitation, chromatography, desalination from the marine organisms Scapharca subcrenata and invent.The present invention adopted novel advanced person biotechnology extraction, ammonium sulfate precipitation, adopted gel permeation chromatography separation, the desalination of Sephade G-25 desalting column to prepare Scapharca subcrenata peptide extract with high anti-cancer activity.The molecular weight that the present invention creatively filters out is that the following peptide constituents of 3000Dal has best pharmacological effect and economical and technical benefit; In order further to prepare Scapharca subcrenata peptides injection, for clinical vein, intervention medication, the present invention has used biological clarification technique, has adopted cryogenic conditions to separate down to guarantee the activity of extract; Use the formed injection of the present invention to have the broad spectrum anticancer curative effect, clinically can be used for treating treatment for cancer such as pulmonary carcinoma, renal carcinoma, gastric cancer, hepatocarcinoma, bladder cancer, carcinoma of prostate, breast carcinoma, leukemia, evident in efficacy, toxic and side effects is little.Through a large amount of development tests of inventor, the present invention has obtained a series of technological progress, and technology advanced person of the present invention, efficient is higher, precision is accurate, purity is higher.
The present invention realizes its goal of the invention by the following technical solutions.
A kind of Scapharca subcrenata antineoplaston extract, it is the extract for preparing by the following method: fresh and alive Scapharca subcrenata soft tissue is cleaned smashed to pieces homogenate, through extract at low temperature, centrifugal removing slag, biological clarifier absorption, freeze and separate, obtain extract freeze-drying powder after the freeze drying box lyophilizing, again with extract freeze-drying powder through ammonium sulfate precipitation, the Superdex75 gel permeation chromatography, merging molecular weight is that the following eluting peak of 3000Dal just obtains the peptides eluent, last lyophilizing eluent, use the water for injection dissolved freeze-dried powder, through the desalination of Sephade G-25 desalting column, extracting solution obtains the Scapharca subcrenata peptides through the lyophilizing of vacuum freeze-drying machine after the desalination.
Aforesaid a kind of Scapharca subcrenata antineoplaston extract, homogenate granularity in the described preparation process is 20um-50um, the freeze dried freeze temperature of freeze drying box is that 30 ℃～40 ℃, freeze-drying time are 25-35 hour at-30 ℃～-40 ℃, sublimation temperature, the substance characteristics of this Scapharca subcrenata peptides is that molecular weight is less than 3000Dal, pH value is 5.5～7.5, easily be dissolved in water, there is absorption maximum at the 280nm place.
A kind of Scapharca subcrenata antineoplaston preparation method of extract, it is fresh and alive Scapharca subcrenata soft tissue to be cleaned smash homogenate to pieces, through extract at low temperature, centrifugal removing slag, biological clarifier absorption, freeze and separate, obtain extract freeze-drying powder after the freeze drying box lyophilizing, again with extract freeze-drying powder through ammonium sulfate precipitation, the Superdex75 gel permeation chromatography, merging molecular weight is that the following eluting peak of 3000Dal just obtains the peptides eluent, last lyophilizing eluent, use the water for injection dissolved freeze-dried powder, through the desalination of SephadeG-25 desalting column, extracting solution obtains the Scapharca subcrenata peptides through the lyophilizing of vacuum freeze-drying machine after the desalination.
Aforesaid a kind of Scapharca subcrenata antineoplaston preparation method of extract, the concrete technology of this preparation method is as follows:
(1), fresh and alive Scapharca subcrenata organism is cleaned, shelled and get soft tissue and clean, smash to pieces the back with the homogenate of colloid mill cryogrinding, granularity is 20um-50um, and temperature is below 4 ℃.
(2), the clean material after the homogenate extracts 1～2 time with weight ratio with the fresh water for injection of producing that is chilled to below 6 ℃ at 1: 1, used blender was answered slow extraction 3-5 hour.
(3), extracting solution after centrifugal removing slag, after adding biological clarifier III type by volume the order of A adding stirring and evenly mixing after by first B, fully absorption in 10-14 hour is placed in cold preservation, wherein the addition of B is 1%～3%, the addition of A is half of B addition.
(4), clear liquor after refrigerated centrifuger separates, separating medium is gone into freeze drying box and is carried out lyophilizing.Freeze temperature is at-30 ℃～-40 ℃, and sublimation temperature is 30 ℃～40 ℃.Freeze-drying time is 25-35 hour, takes out lyophilized powder.
(5), take by weighing a certain amount of Scapharca subcrenata extract freeze-drying powder, be dissolved in buffer A (20mMTris.Cl, 50mMNaCl, 2mEDTA, pH8.0) in, the w/v of lyophilized powder and buffer A is 6: 1-10: 1 (g/L), carry out ammonium sulfate precipitation then, get 20%～60% saturation ammonium sulfate precipitation and be dissolved among the buffer solution A, centrifugal 8-12min under 4 ℃ of 10000g gets supernatant.
(6), on the supernatant Superdex75 gel permeation chromatography post (16 * 60cm), eluent is buffer solution A, and flow velocity is 0.4-0.6ml/min, and the 280nm place is detected, and substep is collected, every pipe 3ml.
(7), merge eluent in each eluting peak.
(8), coarse wool Carnis Arca inflata extract freeze-drying powder can obtain peptides eluting honeybee through above-mentioned purification.
(10), the peptides eluent that obtains is carried out lyophilizing, lyophilized powder.
(11), adopt (2 * 20cm) desalinations of Sephade G-25 desalting column with water for injection dissolving peptides lyophilized powder.Extracting solution is through the lyophilizing of vacuum freeze-drying machine after the desalination, and freeze temperature is at-30 ℃～-40 ℃, and sublimation temperature is 30 ℃～40 ℃.Freeze-drying time is 25-35 hour, gets Scapharca subcrenata peptides lyophilized powder, adopts dry back weight method to carry out quantitatively.
Implement technology of the present invention, can realize purpose of the present invention, can overcome and avoid the shortcoming and defect of prior art, the following peptide constituents of the molecular weight 3000Dal that the present invention creatively filters out has best pharmacological effect and economical and technical benefit; The present invention has used biological clarification technique, has adopted cryogenic conditions to separate down, extract to guarantee the activity of extract; The present invention has adopted the step of desalination behind the first chromatography, and technology advanced person, efficient is higher, precision is accurate, purity is higher.
Record by analysis, the substance characteristics of this Scapharca subcrenata peptide extract be molecular weight less than below the 3000Dal, pH value is 5.5～7.5, easily is dissolved in water, there is absorption maximum at the 280nm place.
The present invention can also further prepare the agent of Scapharca subcrenata antineoplaston compound injection, for clinical vein, intervention medication, use the formed injection of the present invention to have the broad spectrum anticancer curative effect, clinically can be used for treating treatment for cancer such as pulmonary carcinoma, renal carcinoma, gastric cancer, hepatocarcinoma, bladder cancer, carcinoma of prostate, breast carcinoma, leukemia, evident in efficacy, toxic and side effects is little.
The concrete technology of preparation Scapharca subcrenata antineoplaston compound injection agent is as follows:
It is an amount of to take by weighing Scapharca subcrenata antineoplaston chemical compound lyophilized powder, being dissolved in proper amount of fresh produces in the water for injection that is chilled to below 10 ℃, make it fully dissolve mixing, after adding biological clarifier III type by volume the order of A adding stirring and evenly mixing after by first B, cold preservation is placed 10-14 hour, and fully the absorption back is centrifugal, wherein the addition of B is 1%～3%, the addition of A is half of B addition, centrifugal liquid through microporous filter membrane degerming ultrafiltration fill in the 2ml ampoule, through pyrogen, aseptic, clarity, clarity, loading amount, content etc. after the assay was approved, for clinical vein, get involved medication, be used for pulmonary carcinoma, renal carcinoma, gastric cancer, hepatocarcinoma, bladder cancer, carcinoma of prostate, breast carcinoma, multiple treatment for cancer such as leukemia.
The specific embodiment
The invention will be further described below in conjunction with illustrated embodiment.
Take by weighing fresh ocean and catch the Scapharca subcrenata raw material 600g that gets, tap water is cleaned mud, moving to ten thousand grades of clean rooms (temperature control is below 10 ℃) dials and to go shell to take out the software part, purification washes with water to no blood stains, the fresh water for injection of producing (requirement is chilled to below 10 ℃) washing three times, drain away the water, smash soft tissue to pieces clean material that (granularity 1mm) smash to pieces with bruisher and grind low-temperature homogenate (grain is through being 20um-50um) through colloid mill, the clean material of homogenate stirred 4 hours (60 rev/mins keep about 4 ℃ of extracting solution temperature) in 1: 1 at a slow speed under (w/w) ratio adding fresh water for injection of producing (being chilled to below 10 ℃) cryogenic conditions, the centrifugal collection separating medium that removes slag of refrigerated centrifuger (2000 rev/mins), separating medium is centrifugal through tube centrifuge (16000 rev/mins) again, separating medium is collected in the stainless steel cask, transfer pH5.5 in right amount with 1% hydrochloric acid (analytical pure), add clarifier III type, add by the order of A behind the first B that [the B addition is 3% (v/v), the A addition is 1.5% (v/v)] mix, left standstill 12 hours in cold closet (4 ℃), make it fully to adsorb macromole impurity albumen, microorganism and pyrogen, clear liquor is after refrigerated centrifuger separates, separating medium is gone into freeze drying box, through-30 ℃, after the lyophilizing 30 hours, sublimation temperature is 35 ℃, takes out lyophilized powder.
Take by weighing Scapharca subcrenata extract freeze-drying powder 0.4g, be dissolved in 50ml buffer A (20mMTris.Cl, 50mMNaCl, 2mEDTA, pH8.0) carry out ammonium sulfate precipitation in then, get 20%～60% saturation ammonium sulfate precipitation and be dissolved among the 2ml buffer solution A, 4 ℃ of centrifugal 10min of 10000g get supernatant.(16 * 60cm), eluent is buffer solution A to Superdex75 gel permeation chromatography post on the supernatant, and flow velocity is 0.5ml/min, and the 280nm place is detected, and substep is collected, every pipe 3ml.Merge the eluent in each eluting peak, get 10ul and carry out the SDS-PAGE analysis.The Scapharca subcrenata extract freeze-drying powder can obtain peptide class eluting peak through above-mentioned purification.The peptide class eluent that obtains is carried out lyophilizing, obtain lyophilized powder.Adopt (2 * 20cm) desalinations of Sephade G-25 desalting column with the water for injection dissolved freeze-dried powder.Solution after the desalination is through vacuum drying technique lyophilizing (freeze temperature is-35 ℃, freeze-drying time 30 hours, sublimation temperature is 30 ℃).
Take by weighing Scapharca subcrenata peptide class lyophilized powder 100mg, be dissolved in that 100ml is fresh to be produced in the water for injection that is chilled to below 10 ℃, make it fully dissolve mixing, add by volume biological clarifier III type by first B after the order of A add that (addition of B is 1%～3%, the addition of A is half of B addition) behind the stirring and evenly mixing, cold preservation is placed 12 hours, and fully the absorption back is centrifugal, centrifugal liquid through microporous filter membrane degerming ultrafiltration fill in the 2ml ampoule, through assay (by containing peptide 0.9～1.1mg/ml), the thermal source experiment, Micro biological Tests and clarity, clarity, pH value is up to the standards, clinical in vein, get involved medication, be used for: pulmonary carcinoma, renal carcinoma, gastric cancer, hepatocarcinoma, bladder cancer, carcinoma of prostate, breast carcinoma, treatment for cancer such as leukemia.
Compared with the prior art the present invention has many technological progresses:
1, adopt biological clarifier to remove the macromole foreign protein in the extraction process in the present invention early stage, and centrifugal process adopts separation and Extraction under the cryogenic conditions, preserving activity, and existing technology adopts centrifugal extraction under the generic condition, has kept the composition of macromolecule.
2, crude extract of the present invention is preserved raw material through freeze drying technology, makes in its separation and purification afterwards, and the exposure solution time is short, and complete components also is convenient to later extraction separation, and the existing technology exposure solution time is long, and composition is imperfect.
3, the present invention after gel permeation chromatography separates, carries out desalination through Sephade G-25 desalting column earlier again, so desalination is thorough, the purity of extract improves; Adopt the vacuum freeze drying technology lyophilizing to preserve raw material, make things convenient for preparation, it is active that extract is fully preserved at low temperatures; And existing technology extracting solution carries out the gel filtration chromatography separation again after desalination, concentrate drying, and inconvenient preparation, purity drop, and be unfavorable for that the extract preservation is active.
4, this invention gained composition is a peptides, has further obtained the material concrete property of this peptides by analysis: molecular weight is less than 3000Dal, and pH value is 5.5～7.5, easily is dissolved in water, and there is absorption maximum at the 280nm place; And existing technology gained composition is a polypeptide protein, and molecular weight ranges is wide in range, can't implement.
5, this invention further adopts the Scapharca subcrenata peptides clarification technique depyrogenation, hyperfiltration technique degerming to be prepared into injection, for clinical vein, get involved medication, and clear and definite antitumor spectra and curative effect.
The injection antitumor spectra of the prepared one-tenth of this invention is wide, through various tests and clinical discovery are should the anti-tumor of invention evident in efficacy, toxic and side effects is little.The raw materials used source of the invention is wide, be easy to get, and extracts, the separation process power consumption is low, and environment is not produced any pollution, and is easy to prepare.The present invention has determined the anti-tumor active substance in the Scapharca subcrenata extract, clear and definite its effective site, determined molecular weight (molecular weight is less than 3000Dal), pH value scope (5.5～7.5), and it is soluble in water to separate the chemical compound that obtains, there is absorption maximum at the 280nm place.
Claims (4)
1, a kind of Scapharca subcrenata antineoplaston extract, it is characterized in that it is the extract for preparing by the following method: fresh and alive Scapharca subcrenata soft tissue is cleaned smashed to pieces homogenate, through extract at low temperature, centrifugal removing slag, biological clarifier absorption, freeze and separate, obtain extract freeze-drying powder after the freeze drying box lyophilizing, again with extract freeze-drying powder through ammonium sulfate precipitation, the Superdex75 gel permeation chromatography, merging molecular weight is that the following eluting peak of 3000Dal just obtains the peptides eluent, last lyophilizing eluent, use the water for injection dissolved freeze-dried powder, through the desalination of Sephade G-25 desalting column, extracting solution obtains the Scapharca subcrenata peptide extract through the lyophilizing of vacuum freeze-drying machine after the desalination.
2, a kind of Scapharca subcrenata antineoplaston extract according to claim 1, it is characterized in that described homogenate granularity is 20um-50um, the freeze dried freeze temperature of freeze drying box is that 30 ℃～40 ℃, freeze-drying time are 25-35 hour at-30 ℃～-40 ℃, sublimation temperature.
3, a kind of Scapharca subcrenata antineoplaston preparation method of extract, it is characterized in that it is fresh and alive Scapharca subcrenata soft tissue to be cleaned smash homogenate to pieces, through extract at low temperature, centrifugal removing slag, biological clarifier absorption, freeze and separate, obtain extract freeze-drying powder after the freeze drying box lyophilizing, again with extract freeze-drying powder through ammonium sulfate precipitation, the Superdex75 gel permeation chromatography, merging molecular weight is that the following eluting peak of 3000Dal just obtains the peptides eluent, last lyophilizing eluent, use the water for injection dissolved freeze-dried powder, through the desalination of Sephade G-25 desalting column, extracting solution obtains the Scapharca subcrenata peptides through the lyophilizing of vacuum freeze-drying machine after the desalination.
4, a kind of Scapharca subcrenata antineoplaston preparation method of extract according to claim 3 is characterized in that the concrete technology of described preparation method is as follows:
(1), fresh and alive Scapharca subcrenata organism is cleaned, shelled and get soft tissue and clean, smash to pieces the back with the homogenate of colloid mill cryogrinding, granularity is 20um-50um, and temperature is below 4 ℃.
(2), the clean material after the homogenate extracts 1～2 time with weight ratio with the fresh water for injection of producing that is chilled to below 6 ℃ at 1: 1, used blender was answered slow extraction 3-5 hour.
(3), extracting solution after centrifugal removing slag, after adding biological clarifier III type by volume the order of A adding stirring and evenly mixing after by first B, fully absorption in 10-14 hour is placed in cold preservation, wherein the addition of B is 1%～3%, the addition of A is half of B addition.
(4), clear liquor after refrigerated centrifuger separates, separating medium is gone into freeze drying box and is carried out lyophilizing.Freeze temperature is at-30 ℃～-40 ℃, and sublimation temperature is 30 ℃～40 ℃, and freeze-drying time is 25-35 hour, takes out lyophilized powder.
(5), take by weighing a certain amount of Scapharca subcrenata extract freeze-drying powder, be dissolved in buffer A (20mMTris.Cl, 50mMNaCl, 2mEDTA, pH8.0) in, the w/v of lyophilized powder and buffer A is 6: 1-10: 1 (g/L), carry out ammonium sulfate precipitation then, get 20%～60% saturation ammonium sulfate precipitation and be dissolved among the buffer solution A, centrifugal 8-12min under 4 ℃ of 10000g gets supernatant.
(6), on the supernatant Superdex75 gel permeation chromatography post (16 * 60cm), eluent is buffer solution A, and flow velocity is 0.4-0.6ml/min, and the 280nm place is detected, and substep is collected, every pipe 3ml.
(7), merge eluent in each eluting peak.
(8), coarse wool Carnis Arca inflata extract freeze-drying powder can obtain the peptide compounds eluting peak through above-mentioned purification.
(10), the peptides eluent that obtains is carried out lyophilizing, lyophilized powder.
(11), with water for injection dissolving peptides lyophilized powder, adopt (2 * 20cm) desalinations of Sephade G-25 desalting column, extracting solution is through the lyophilizing of vacuum freeze-drying machine after the desalination, freeze temperature is at-30 ℃～-40 ℃, sublimation temperature is 30 ℃～40 ℃, freeze-drying time is 25-35 hour, gets Scapharca subcrenata peptides lyophilized powder, adopts dry back weight method to carry out quantitatively.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100437974A CN1857717A (en) | 2005-06-17 | 2005-06-17 | Anticancer peptide extract of blood clam and its preparing method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2005100437974A CN1857717A (en) | 2005-06-17 | 2005-06-17 | Anticancer peptide extract of blood clam and its preparing method |
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| CN1857717A true CN1857717A (en) | 2006-11-08 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103265598A (en) * | 2013-06-03 | 2013-08-28 | 广西壮族自治区海洋研究所 | Method for extracting steroidal compounds from Scapharca subcrenata |
| CN103613644A (en) * | 2013-12-11 | 2014-03-05 | 湖南莱拓福生物科技有限公司 | Small peptide lactotransferrin (LTF) with anti-tumor effect and application thereof |
| WO2014161240A1 (en) * | 2013-04-01 | 2014-10-09 | Yu Rongmin | Preparation method and application of anti-tumor active polypeptide component in scapharca subcrenatae |
| CN110343072A (en) * | 2019-07-12 | 2019-10-18 | 浙江海洋大学 | A method of carnosine is extracted from stripped tuna head |
-
2005
- 2005-06-17 CN CNA2005100437974A patent/CN1857717A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014161240A1 (en) * | 2013-04-01 | 2014-10-09 | Yu Rongmin | Preparation method and application of anti-tumor active polypeptide component in scapharca subcrenatae |
| CN103265598A (en) * | 2013-06-03 | 2013-08-28 | 广西壮族自治区海洋研究所 | Method for extracting steroidal compounds from Scapharca subcrenata |
| CN103613644A (en) * | 2013-12-11 | 2014-03-05 | 湖南莱拓福生物科技有限公司 | Small peptide lactotransferrin (LTF) with anti-tumor effect and application thereof |
| CN103613644B (en) * | 2013-12-11 | 2015-09-30 | 湖南莱拓福生物科技有限公司 | A kind of little peptide LTF and application thereof with antitumous effect |
| CN110343072A (en) * | 2019-07-12 | 2019-10-18 | 浙江海洋大学 | A method of carnosine is extracted from stripped tuna head |
| CN110343072B (en) * | 2019-07-12 | 2023-01-31 | 浙江海洋大学 | Method for extracting carnosine from skipjack heads |
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