CN1847841A - Method of detecting content of micro amount of aristolochic acid A in water extract of Chinese medicine - Google Patents
Method of detecting content of micro amount of aristolochic acid A in water extract of Chinese medicine Download PDFInfo
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- CN1847841A CN1847841A CN 200510013310 CN200510013310A CN1847841A CN 1847841 A CN1847841 A CN 1847841A CN 200510013310 CN200510013310 CN 200510013310 CN 200510013310 A CN200510013310 A CN 200510013310A CN 1847841 A CN1847841 A CN 1847841A
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- BBFQZRXNYIEMAW-UHFFFAOYSA-M Aristolochate I Natural products C1=C([N+]([O-])=O)C2=C(C([O-])=O)C=C3OCOC3=C2C2=C1C(OC)=CC=C2 BBFQZRXNYIEMAW-UHFFFAOYSA-M 0.000 title claims abstract description 42
- BBFQZRXNYIEMAW-UHFFFAOYSA-N aristolochic acid I Chemical compound C1=C([N+]([O-])=O)C2=C(C(O)=O)C=C3OCOC3=C2C2=C1C(OC)=CC=C2 BBFQZRXNYIEMAW-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 239000003814 drug Substances 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 239000000284 extract Substances 0.000 title claims abstract description 12
- 239000011347 resin Substances 0.000 claims abstract description 12
- 229920005989 resin Polymers 0.000 claims abstract description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 42
- 239000000945 filler Substances 0.000 claims description 35
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 28
- 239000006286 aqueous extract Substances 0.000 claims description 24
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 18
- 238000011010 flushing procedure Methods 0.000 claims description 14
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 10
- 239000013558 reference substance Substances 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000002250 absorbent Substances 0.000 claims description 8
- 230000002745 absorbent Effects 0.000 claims description 8
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 238000002137 ultrasound extraction Methods 0.000 claims description 6
- 229960000583 acetic acid Drugs 0.000 claims description 5
- 239000003463 adsorbent Substances 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000007791 liquid phase Substances 0.000 claims description 5
- 239000012071 phase Substances 0.000 claims description 5
- 239000003480 eluent Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 230000005526 G1 to G0 transition Effects 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000012362 glacial acetic acid Substances 0.000 claims description 3
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 claims description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 150000004696 coordination complex Chemical class 0.000 claims description 2
- 239000003456 ion exchange resin Substances 0.000 claims description 2
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 239000002594 sorbent Substances 0.000 claims description 2
- 238000003809 water extraction Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 abstract description 10
- 238000005516 engineering process Methods 0.000 abstract description 6
- 239000012488 sample solution Substances 0.000 abstract description 3
- 239000007787 solid Substances 0.000 abstract 2
- 238000004811 liquid chromatography Methods 0.000 abstract 1
- 235000017965 Asarum canadense Nutrition 0.000 description 9
- 235000000385 Costus speciosus Nutrition 0.000 description 9
- 241000606265 Valeriana jatamansi Species 0.000 description 9
- 235000014687 Zingiber zerumbet Nutrition 0.000 description 9
- 238000003556 assay Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 241000758794 Asarum Species 0.000 description 3
- 241001529246 Platymiscium Species 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 241000722953 Akebia Species 0.000 description 1
- 235000007756 Akebia quinata Nutrition 0.000 description 1
- 240000008027 Akebia quinata Species 0.000 description 1
- 241000046585 Aristolochia clematitis Species 0.000 description 1
- 241001369615 Aristolochia fangchi Species 0.000 description 1
- 241000758795 Aristolochiaceae Species 0.000 description 1
- 241001647745 Banksia Species 0.000 description 1
- 241000903946 Clematidis Species 0.000 description 1
- 241000218158 Clematis Species 0.000 description 1
- 241000601164 Clematis orientalis Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000245050 Menispermum Species 0.000 description 1
- 235000006386 Polygonum aviculare Nutrition 0.000 description 1
- 244000292697 Polygonum aviculare Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241001330502 Stephania Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 238000009849 vacuum degassing Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The method of detecting micro content of aristolochic acid A in water extract of Chinese medicine includes extracting the Chinese medicine sample with water to obtain water extract of Chinese medicine, treating the water extract by means of solid extracting technology to obtain sample solution, and high efficiency liquid chromatography detecting, with the stuffing for solid extracting being macroporous resin stuffing. The present invention has accurate determination of micro content of aristolochic acid A in water solution.
Description
Technical field
The present invention relates to the content assaying method of Chinese medicine aqueous extract toxic composition, relate in particular to the content assaying method of aristolochic acid A in the Chinese medicine aqueous extract.
Background technology
Aristolochic acid A is a kind of toxic component that contains in the Chinese medicine, can cause kidney damage.The Chinese medicine that contains aristolochic acid A mainly is aristolochiaceae plant, mainly comprise birthwort birthwort, caulis aristologhiae manshuriensis, Aristolochia fangchi, dutchmanspipe root, herba aristolochiae, Ciliatenerve Knotweed Root, berba aristolochiae mollissimae, the asarum plant root of Chinese wild ginger, wild ginger, Akebia plant akebi, Clematis plant caulis clematidis armandii, the root of Chinese clematis, wind dragon platymiscium caulis sinomenii, the stephania plant root of fangji, radix jurineae platymiscium radix jurineae, moon-seed rhizoma menispermi, the Aplotaxis auriculata platymiscium banksia rose.Pure aristolochic acid A has dissolubility preferably in organic solvents such as methyl alcohol, but the Yishui River hard to tolerate.For the assay of aristolochic acid A in the Chinese medicine, generally be to extract solvent with methyl alcohol, aristolochic acid A is extracted from Chinese crude drug, adopt the higher method of sensitivity again, as high performance liquid chromatography, carry out assay.In the Chinese medicine industrialized producing technology, water is the extraction solvent that often adopts.As if be insoluble in the character of water according to pure aristolochic acid A, aristolochic acid A can not be extracted.But kinds of traditional Chinese medicines is in the solvent co-extracted process with water, ubiquity hydrotropy, altogether effect such as molten, and the aristolochic acid A in the Chinese crude drug still can be extracted on a small quantity.Rationality for the suitability for industrialized production extraction process of studying Chinese medicine is necessary the micro-aristolochic acid A in the aqueous extract is carried out assay.Because aristolochic acid A is insoluble in water, even existence molten altogether, the hydrotropy effect is arranged, the content of aristolochic acid A in aqueous solution is still very low, even adopt the very high efficient liquid-phase chromatography method of sensitivity, and can not detection by quantitative.Therefore, must adopt effective ways, the aristolochic acid A composition of trace concentrates in the centering liquid medicine extract, so that adopt suitable content assaying method, accurately measures the content of aristolochic acid A in the aqueous extract.
(Solid phase extraction, SPE) technology is a physical extraction technology that comprises liquid phase and solid phase to Solid-Phase Extraction.In Solid-Phase Extraction, solid phase is bigger than the solvent of separated and dissolved thing to the absorption affinity of separator.When sample solution passed through adsorbent bed, separator was concentrated in its surface, and other sample composition passes through adsorbent bed.The adsorbent that does not adsorb other sample composition by an adsorptive separation thing, the separator that can obtain high-purity and concentrate.
The present invention unites use with SPE pretreatment technology and high-efficient liquid phase chromatogram technology, success concentrates micro-aristolochic acid A in the aqueous solution, make the sample solution that carries out efficient liquid phase chromatographic analysis contain the aristolochic acid A of high concentration, can accurately measure the micro-aristolochic acid A that contains in the Chinese medicine extract.
Summary of the invention
Purpose of the present invention is exactly the content assaying method that micro-aristolochic acid A in a kind of Chinese medicine aqueous extract will be provided.
The present invention is implemented by following scheme: the aqueous extract of Chinese medicine is used the content of high-performance liquid chromatogram determination aristolochic acid A again after solid phase extraction techniques is handled.
The aqueous extract of above-mentioned Chinese medicine can adopt heating or ultrasonic Extraction mode, and the centrifugal or filtration of extract is got supernatant or filtrate as extract.The condition of above-mentioned high-performance liquid chromatogram determination can adopt the octadecyl silane stationary phase, acetonitrile-aqueous acetic acid system flow phase.
The aqueous extract of above-mentioned Chinese medicine is handled through solid phase extraction techniques, is the solid phase extraction column that the aqueous extract of Chinese medicine is handled well on directly, with 10～70% methyl alcohol or alcohol flushing, use the acetone wash-out at last, collect the acetone eluent, constant volume, shake up, promptly get solution for test usefulness.
The disposal route of above-mentioned solid phase extraction pillar places in internal diameter 0.5～3cm glass pillar for taking by weighing 30～400 order solid phase extraction fillers, 0.2～5g, add methyl alcohol, ethanol or acetone 5～50mL flushing after, water 5～50mL washes again, and is standby.The filler of solid phase extraction column can be graphitic carbon reverse phase filler, ion exchange resin filler, metal complex adsorbent filler, bonded silica gel filler, polymer absorbant filler, immune affinity sorbent filler, molecule embedded polymer thing filler, macroporous absorbent resin filler.Preferred macroporous absorbent resin SPE filler.Macroporous absorbent resin SPE filler can be GDX-101 filler, AB-8 filler or other macroporous absorbent resin filler; Preferred GDX-101SPE filler.
After adopting centering liquid medicine extract of the present invention to carry out the SPE processing, the micro-aristolochic acid A in the aqueous solution is concentrated, and can adopt high performance liquid chromatography to carry out accurate assay.Do not use SPE technical finesse Chinese medicine aqueous extract, directly adopt high performance liquid chromatography to carry out assay the Chinese medicine aqueous extract, aristolochic acid A chromatographic peak peak height is lower than minimum quantitative limit, can not accurately measure aristolochic acid A content, and the result is inaccurate.
Description of drawings
Fig. 1: the HPLC chromatogram that uses SPE technical finesse sample.
Fig. 2: the HPLC chromatogram that does not use SPE technical finesse sample.
Fig. 3: the HPLC chromatogram of aristolochic acid A reference substance.
Embodiment
Enumerate embodiment below and further describe the present invention, this embodiment only is used to the present invention is described and the present invention is not limited.
Embodiment one and Comparative Examples: aristolochic acid A assay in the root of Chinese wild ginger aqueous extract
The root of Chinese wild ginger is the Chinese medicine that contains aristolochic acid A.In suitability for industrialized production, often adopt water as extracting solvent.Pure aristolochic acid A is insoluble in water, but, because other composition is to hydrotropy, the molten effect altogether of aristolochic acid A in the root of Chinese wild ginger, aristolochic acid A in the root of Chinese wild ginger can be extracted on a small quantity, because aristolochic acid A is a kind of toxic component, is necessary accurately to measure the content of aristolochic acid A in the aqueous extract.Can accurately measure the content of aristolochic acid A in the aqueous extract with the inventive method.
1, instrument, medicine and reagent
Agilent 1100 type high performance liquid chromatographs, configuration quaternary pump, online vacuum degassing machine, diode array detector, column oven.The root of Chinese wild ginger (day scholar's power modern Chinese herbal medicine Resources Co., Ltd provides).The aristolochic acid A reference substance is purchased in Nat'l Pharmaceutical ﹠ Biological Products Control Institute (lot number: 0746-200103, assay is used).Methyl alcohol (analyze pure, Tianjin chemical reagent two factories), glacial acetic acid (analyze pure, Shanghai chemical reagents corporation of Chinese Medicine group), acetonitrile (chromatographically pure, Merck company), purified water.Macroreticular resin filler GDX-101 (available from Tianjin chemical reagent two factories).
2, chromatographic condition
Chromatographic column is Agilent Zorbax SB-C
18(250mm * 4.6mm, 5 μ m), 30 ℃ of column temperatures.With acetonitrile-1% glacial acetic acid aqueous solution (39: 61) is moving phase, and flow velocity is 0.8mLmin-1, and the detection wavelength is 317nm.
3, the preparation of solution
(1) reference substance solution: it is an amount of to get the aristolochic acid A reference substance, uses dissolve with methanol, constant volume, and making concentration is the aristolochic acid A reference substance solution of 5.25 μ gmL-1.
(2)-1 the need testing solution of embodiment one: get Asarum medicinal materials powder 5g, the accurate title, decide, put in the 250mL flask, add water 200mL, ultrasonic Extraction 30min, centrifugal, get supernatant 100mL, on the GDX-101 pillar handled well (take by weighing 50～100 order GDX-101 filler 1g, place in the internal diameter 1cm glass pillar, add methyl alcohol 20mL flushing, water 20mL flushing again, standby), the flow velocity of sample and elution process is not more than 0.7mLmin-1 in the control, wash with 70% methyl alcohol 10mL, with acetone 10mL flushing, collect acetone solution again, be settled to 10mL, shake up, promptly.
(2)-2 Comparative Examples need testing solution: get Asarum medicinal materials powder 5g, accurate claim surely, put in the 250mL flask, add water 200mL, ultrasonic Extraction 30min, centrifugal, get supernatant, promptly.
4, measure
Draw each 15 μ L of reference substance solution and need testing solution respectively, inject high performance liquid chromatograph, measure, promptly.
5, result
Aristolochic acid A assay result in table 1 root of Chinese wild ginger aqueous extract
| Batch | Aristolochic acid A content (μ g/100ml aqueous extract) | |
| Use SPE | Do not use SPE | |
| 20020508 20030203 20030601 20031218 20040306 20040907 20041002 20050110 | 36.7 29.1 12.8 16.8 22.8 15.9 36.7 29.1 | Peak height is lower than minimum quantitative limit, quantitatively peak height is lower than minimum quantitative limit, quantitatively peak height is lower than minimum quantitative limit, quantitatively peak height is lower than minimum quantitative limit, quantitatively peak height is lower than minimum quantitative limit, quantitatively peak height is lower than minimum quantitative limit, quantitatively peak height is lower than minimum quantitative limit, quantitatively peak height is lower than minimum quantitative limit, can not be quantitative |
From table, can find out, after using SPE technical finesse sample, among the sample chromatogram figure, aristolochic acid A chromatographic peak peak height can carry out accurately quantitatively the aristolochic acid A in the root of Chinese wild ginger aqueous solution much larger than the peak height of minimum quantitative limit (the average peak height of signal noise 10 times) (see figure 1); And do not use SPE technical finesse sample, during direct injection analysis, the aristolochic acid A peak height is less than the peak height of minimum quantitative limit, quantitatively (see figure 2).
Embodiment two: aristolochic acid A assay in the caulis aristologhiae manshuriensis aqueous extract
The preparation of need testing solution: get caulis aristologhiae manshuriensis medicinal powder 6g, the accurate title, decide, and puts in the 250mL flask, adds water 200mL, 30min is extracted in heating, centrifugal, get supernatant 100mL, on the AB-8 pillar handled well (take by weighing 200～400 order AB-8 filler 4g, place in the internal diameter 2cm glass pillar, add methyl alcohol 40mL flushing, water 40mL flushing again, standby; Macroreticular resin filler AB-8 is available from Chemical Plant of Nankai Univ.), the flow velocity of sample and elution process is not more than 0.7mLmin-1 in the control, with 30% methyl alcohol 10mL flushing, with acetone 10mL flushing, collects acetone solution again, is settled to 10mL, shakes up, promptly.
Other is all with embodiment one
The result: aristolochic acid A content is 126 μ g in the aqueous extract.
Claims (9)
1. the content assaying method of aristolochic acid A in the Chinese medicine aqueous extract is characterized in that, the aqueous extract of Chinese medicine is used the content of high-performance liquid chromatogram determination aristolochic acid A again after solid phase extraction techniques is handled.
2. content assaying method according to claim 1 is characterized in that, the aqueous extract of described Chinese medicine is heating of Chinese medicine water or ultrasonic Extraction, and the centrifugal or filtration of extract is got supernatant or filtrate as extract; What the condition determination of described high performance liquid chromatography adopted is the octadecyl silane stationary phase, acetonitrile-aqueous acetic acid system flow phase.
3. content assaying method according to claim 1, it is characterized in that, the aqueous extract of described Chinese medicine is handled through solid phase extraction techniques, be the solid phase extraction column that the aqueous extract of Chinese medicine is handled well on directly,, use the acetone wash-out at last with 10～70% methyl alcohol or alcohol flushing, collect the acetone eluent, constant volume shakes up, and promptly gets the solution for high performance liquid chromatography test usefulness.
4. content assaying method according to claim 3, it is characterized in that, the disposal route of described solid phase extraction column is for taking by weighing 30～400 order solid phase extraction fillers, 0.2～5g, place in internal diameter 0.5～3cm glass pillar, after adding methyl alcohol, ethanol or acetone 5～50mL flushing, water 5～50mL flushing again, standby.
5. content assaying method according to claim 4, it is characterized in that the filler of described solid phase extraction column is graphitic carbon reverse phase filler, ion exchange resin filler, metal complex adsorbent filler, bonded silica gel filler, polymer absorbant filler, immune affinity sorbent filler, molecule embedded polymer thing filler, macroporous absorbent resin filler.
6. content assaying method according to claim 5 is characterized in that, the filler of described solid phase extraction column is the macroporous absorbent resin filler.
7. content assaying method according to claim 6 is characterized in that, the filler of described solid phase extraction column is macroporous absorbent resin GDX-101 filler or macroporous absorbent resin AB-8 filler.
8. content assaying method according to claim 1 is characterized in that, described method is for getting Chinese medicine 0.2～2g, add water, heating or ultrasonic Extraction, the centrifugal or filtration of extract, get supernatant or filtrate, on the solid phase extraction column handled well, earlier with 10～70% methyl alcohol or alcohol flushing, use the acetone wash-out again, collect the acetone eluent, constant volume shakes up, and promptly gets need testing solution; Get the aristolochic acid A reference substance and be mixed with reference substance solution; High-efficient liquid phase chromatogram condition adopts the octadecyl silane stationary phase with acetonitrile-the glacial acetic acid aqueous solution system flow mutually; Need testing solution and reference substance solution are injected high performance liquid chromatograph respectively, measure, promptly.
9. content assaying method according to claim 8 is characterized in that, Chinese medicine 0.2～2g is got in being prepared as of described need testing solution, and accurate the title decides, and puts in the flask, adds water 50～300mL, and heating or ultrasonic Extraction 0.2～2.5h are put cold, centrifugal; Get supernatant, on the macroreticular resin pillar handled well, the flow velocity of sample and elution process with 10～70% methyl alcohol or alcohol flushing, is used the acetone wash-out again in the control in 0.3～2mLmin-1 scope, collects the acetone eluent, constant volume shakes up, and promptly gets need testing solution.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2005100133108A CN1847841B (en) | 2005-04-12 | 2005-04-12 | Method of detecting content of micro amount of aristolochic acid A in water extract of Chinese medicine |
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| CN2005100133108A CN1847841B (en) | 2005-04-12 | 2005-04-12 | Method of detecting content of micro amount of aristolochic acid A in water extract of Chinese medicine |
Publications (2)
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| CN1847841A true CN1847841A (en) | 2006-10-18 |
| CN1847841B CN1847841B (en) | 2010-04-28 |
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| CN2005100133108A Expired - Fee Related CN1847841B (en) | 2005-04-12 | 2005-04-12 | Method of detecting content of micro amount of aristolochic acid A in water extract of Chinese medicine |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063800A (en) * | 2013-01-01 | 2013-04-24 | 吉林紫鑫药业股份有限公司 | Method for detecting active ingredients of Longdan Xiegan particles |
| CN106124461A (en) * | 2016-06-08 | 2016-11-16 | 四川大学 | The method for quick of Aristolochic Acid |
| CN106198771A (en) * | 2015-05-27 | 2016-12-07 | 北京大学 | Measure the HPLC-FLD-DAD of two breeds of horses Semen Oroxyli acid-DNA adduct in tissue simultaneously and analyze method |
| CN106872247A (en) * | 2015-12-10 | 2017-06-20 | 中国科学院大连化学物理研究所 | A kind of preprocess method for being enriched with aristolochic acid |
| CN117147738A (en) * | 2023-10-31 | 2023-12-01 | 吉林市双士药业有限公司 | Method for detecting aristolochic acid I in refreshment and reconstruction pill |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2646545C3 (en) * | 1976-10-15 | 1980-03-20 | Dr. Madaus & Co, 5000 Koeln | Process for the production of aristolochic acids |
| CN100422734C (en) * | 2004-04-27 | 2008-10-01 | 沈阳药科大学 | Detection method for toxicological harmless traditional Chinese herbal Asarum |
-
2005
- 2005-04-12 CN CN2005100133108A patent/CN1847841B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063800A (en) * | 2013-01-01 | 2013-04-24 | 吉林紫鑫药业股份有限公司 | Method for detecting active ingredients of Longdan Xiegan particles |
| CN106198771A (en) * | 2015-05-27 | 2016-12-07 | 北京大学 | Measure the HPLC-FLD-DAD of two breeds of horses Semen Oroxyli acid-DNA adduct in tissue simultaneously and analyze method |
| CN106872247A (en) * | 2015-12-10 | 2017-06-20 | 中国科学院大连化学物理研究所 | A kind of preprocess method for being enriched with aristolochic acid |
| CN106124461A (en) * | 2016-06-08 | 2016-11-16 | 四川大学 | The method for quick of Aristolochic Acid |
| CN106124461B (en) * | 2016-06-08 | 2018-08-21 | 四川大学 | The rapid detection method of aristolochic acid |
| CN117147738A (en) * | 2023-10-31 | 2023-12-01 | 吉林市双士药业有限公司 | Method for detecting aristolochic acid I in refreshment and reconstruction pill |
| CN117147738B (en) * | 2023-10-31 | 2024-02-27 | 吉林市双士药业有限公司 | Method for detecting aristolochic acid I in refreshment and reconstruction pill |
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| Publication number | Publication date |
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| CN1847841B (en) | 2010-04-28 |
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