CN1840685A - Process for fermentation preparation of carotinoid in aweto - Google Patents
Process for fermentation preparation of carotinoid in aweto Download PDFInfo
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- CN1840685A CN1840685A CN 200610018266 CN200610018266A CN1840685A CN 1840685 A CN1840685 A CN 1840685A CN 200610018266 CN200610018266 CN 200610018266 CN 200610018266 A CN200610018266 A CN 200610018266A CN 1840685 A CN1840685 A CN 1840685A
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Abstract
The disclosed technique to prepare carotenoid from cordycepin comprises: deep fermenting the material to put the fermentation liquor in container for light illumination with 360-470nm wavelength and 2~40 mumol m-2 s-1 light intensity or 20-80W common light; collecting the mycelium on liquor surface to grind and treat with HCl, water bathing at 80-100Deg, and extracting with alcohol. This invention has high yield. The product has well reliability for wide application.
Description
Technical field
The present invention relates to a kind of fermentative carotenoid production technique, relate in particular to a kind of from Cordyceps militaris (L.) Link. the technology of fermentative production carotenoid.
Background technology
Carotenoid (carotenoid) tool is anticancer, the effect of anti-cardiovascular disease, precursor as vitamin A, help prevention and radical cure vitamin(e) A deficiency, with its gorgeous yellow, orange red or red, be widely used in fields such as food, medicine, makeup again as tinting material and pigment additive.The production of present carotenoid derives from plant and chemosynthesis mostly, produce the factor affecting that carotenoid is subjected to aspects such as plant origin, growth cycle length, seasonality and productive rate from plant, and there is the potential hazard to human body in chemosynthesis.
Cordyceps militaris (L.) Link. fungus (Corduceps militaris L) has another name called Cordyceps militaris (L.) Link., has the dietary function identical with Cordyceps sinensis, has qi-restoratives, beneficial vital essence, protects effects such as lung, kidney-nourishing, hemostasis and phlegm, strengthening by means of tonics.Yet there are no report in the relevant at present research at home and abroad is material with the Cordyceps militaris (L.) Link., carries out the fermentative production of carotenoid.
Summary of the invention
The objective of the invention is to: overcome deficiency on the prior art, provide a kind of Cordyceps militaris (L.) Link. to be used to ferment and carry out the technology of mass production Cordyceps militaris (L.) Link. carotenoid.
Technology of the present invention may further comprise the steps: at first Cordyceps militaris (L.) Link. is carried out submerged fermentation, will contain the mycelial fermented liquid of Cordyceps militaris (L.) Link. again and place in the container, fermented liquid is carried out illumination, adopt the rayed of 360～470nm wavelength, intensity of illumination is 2～40 μ molm
-2S
-1, perhaps, collect the Cordyceps militaris (L.) Link. fungus filament on the fermented liquid liquid level with common light or the fluorescent lamp irradiation of 20～80W; Again the Cordyceps militaris (L.) Link. fungus filament is ground, use the salt acid treatment, 80～100 ℃ of water-baths, the carotenoid in the pure edible ethanol extracting mycelium obtains dissolved carotenoid, and the carotenoid that is dissolved in the alcohol carries out 40～80 ℃ of distillations, removes alcohol, promptly; The alcohol that perhaps will be dissolved with Cordyceps militaris (L.) Link. carotenoid places 0～-30 ℃, and carotenoid is separated out at low temperatures.。
Fermented liquid of the present invention is prepared by weight by following component: 1～3% sucrose, and 1～2.5% Semen Maydis powder, 2～8% soyflour, 0.1～0.5% potassium primary phosphate, fermented liquid include the Mg that concentration is 0.01～0.05mol/L
2+Surplus is a water.
The temperature of fermenting process in the technology of the present invention and illumination process all is controlled between 23～30 ℃, wherein with 25～28 ℃ for well.
Technology preferred plan of the present invention is: fresh mycelia grinds, the 1g fresh mycelia adds 10mL 3N hydrochloric acid soln, soaks 1～2.5h under the room temperature, water-bath 4min in the boiling water, cooling rapidly, 5, the centrifugal 10min of 000r/min, twice of distilled water washing, add 10mL alcohol vibration 30min and extract carotenoid, 5, the centrifugal 15min of 000r/m gets supernatant liquor.
Mg of the present invention
2+Available sal epsom or magnesium chloride obtain, and fermented liquid pH value is 6.5～7.5.After the illumination 3～10 days, can be with fermented liquid～3 time mycelium results.
Technology of the present invention has the following advantages:
(1) carries out the production of carotenoid by the fermentation of Cordyceps militaris (L.) Link., can obtain a large amount of carotenoid that can be used for a plurality of Application Areass at short notice;
(2) the carotenoid production technique that is obtained by present method production is simple, only need carry out short period of time fermentation and short period of time illumination, starting material in the fermentation obtain easily, and working condition is not harsh yet, have avoided producing the adverse consequences that carotenoid may bring by chemosynthesis;
(3) carotenoid that is obtained has extraordinary stability, is not easy during as additive or other purposes to decompose;
(4) most importantly Cordyceps militaris (L.) Link. carotenoid can present zingy orange-yellowly, and this carotenoid obtains by fermentation, is natural product, is well suited for developing and applying as natural pigment additive; Also can be used as the vitamin A raw material in addition, make an addition in medicine and the food.
(5) carry out the production of Cordyceps militaris (L.) Link. carotenoid with this method, the mycelia that can reach every gram fresh weight is extracted the carotenoid of about 300 to 400 micrograms, and its yield is higher.
Embodiment
The present invention is described in detail below in conjunction with embodiment.
Embodiment 1:
Get 2 kilograms of sucrose, 1.5 kilograms of Semen Maydis powder, 5 kilograms of soyflours, 0.3 kilogram, 0.4 kilogram potassium primary phosphate of sal epsom, the NaOH of 1N transfers pH7.0, and water is added to cumulative volume 100L, and 25 ℃ of fermentation culture produced a large amount of Cordyceps militaris (L.) Link. fungus filaments in 5 days.Again fermented liquid is placed aseptic container, 10 μ molm
-2S
-1The blue light illumination of intensity fermentation liquid level, that collects the fermented liquid surface after 3～5 days becomes orange-yellow mycelium.
Fresh mycelia grinds, and the 1g fresh mycelia adds 10mL 3N hydrochloric acid soln, soaks 2h under the room temperature, 100 ℃ of water-bath 4min, cooling rapidly, 5, the centrifugal 10min of 000r/min, distilled water washing twice adds 10mL alcohol vibration 30min and extracts carotenoid, 5, the centrifugal 15min of 000r/m, get supernatant liquor, alcohol is removed in 60 ℃ of distillations, and carotenoid is slightly carried in being of staying.The alcohol that perhaps will be dissolved with Cordyceps militaris (L.) Link. carotenoid places-20 ℃, and carotenoid is separated out at low temperatures, also can obtain purer carotenoid.
Embodiment 2:
Get 1 kilogram of sucrose, 2.5 kilograms of Semen Maydis powder, 7.5 kilograms of soyflours, 0.1 kilogram and 0.5 kilogram potassium primary phosphate of sal epsom, the NaOH of 1N transfers pH6.5, and water is added to cumulative volume 100L, and 23 ℃ of fermentation culture produced a large amount of Cordyceps militaris (L.) Link. fungus filaments in 8 days.Fermented liquid is placed aseptic container, the 40W fluorescent lamp is in 40cm place irradiation directly over the fermentation liquid level again, and collection fermented liquid surface becomes orange-yellow mycelium after 5 days; Still can grow new mycelium on the results secondary fermentation liquid, can gather in the crops once more after 3～5 days.
Fresh mycelia grinds, and the 1g fresh mycelia adds 5mL alcohol vibration 1h and extracts carotenoid, and 15, the centrifugal 1min of 000r/m gets supernatant liquor, and alcohol is removed in 75 ℃ of distillations, and carotenoid is slightly carried in being of staying.
Embodiment 3:
Get 3 kilograms of sucrose, 1 kilogram of Semen Maydis powder, 2.5 kilograms of soyflours, 0.4 kilogram, 0.15 kilogram potassium primary phosphate of sal epsom, the NaOH of 1N transfers pH7.5, and water is added to cumulative volume 100L, and 28 ℃ of fermentation culture produced a large amount of Cordyceps militaris (L.) Link. fungus filaments in 3 days.Fermented liquid is placed aseptic container, the 60W fluorescent lamp is in 100cm place irradiation directly over the fermentation liquid level again, and collection fermented liquid surface becomes orange-yellow mycelium after 7 days.
Fresh mycelia grinds, and the 1g fresh mycelia adds 15mL 2.5N hydrochloric acid soln, soaks 0.5h under the room temperature, 80 ℃ of water-bath 20min, cooling rapidly, 3, the centrifugal 20min of 000r/min, distilled water washing twice adds 20mL alcohol vibration 30min and extracts carotenoid, 3, the centrifugal 20min of 000r/m, get supernatant liquor, alcohol is removed in 80 ℃ of distillations, and carotenoid is slightly carried in being of staying.The alcohol that perhaps will be dissolved with Cordyceps militaris (L.) Link. carotenoid places-20 ℃, and carotenoid is separated out at low temperatures, also can obtain purer carotenoid.
Embodiment 4:
Get 3 kilograms of sucrose, 1.5 kilograms of Semen Maydis powder, 2.5 kilograms of soyflours, 0.4 kilogram, 0.4 kilogram potassium primary phosphate of sal epsom, the NaOH of 1N transfers pH7.2, and water is added to cumulative volume 100L, and 26 ℃ of fermentation culture produced a large amount of Cordyceps militaris (L.) Link. fungus filaments in 5 days.Fermented liquid is placed aseptic container, the 20W fluorescent lamp shines directly over the 30cm fermented liquid again, and that collects the fermented liquid surface after 3 ~ 5 days becomes orange-yellow mycelium.
Fresh mycelia grinds, and the 1g fresh mycelia adds 8mL 3.5N hydrochloric acid soln, soaks 4.5h under the room temperature, 90 ℃ of water-bath 15min, cooling rapidly, 10, the centrifugal 3min of 000r/min, distilled water washing twice adds 12mL alcohol and leaves standstill 20h extraction carotenoid, 10, the centrifugal 3min of 000r/m, get supernatant liquor, alcohol is removed in 45 ℃ of distillations, and carotenoid is slightly carried in being of staying.The alcohol that perhaps will be dissolved with Cordyceps militaris (L.) Link. carotenoid places-30 ℃, and carotenoid is separated out at low temperatures, also can obtain purer carotenoid.
Embodiment 5:
Get 2.5 kilograms of sucrose, 1 kilogram of Semen Maydis powder, 4 kilograms of soyflours, 0.35 kilogram, 0.45 kilogram potassium primary phosphate of sal epsom, the NaOH of 1N transfers pH6.8, and water is added to cumulative volume 100L, and 25 ℃ of fermentation culture produced a large amount of Cordyceps militaris (L.) Link. fungus filaments in 5 days.Again fermented liquid is placed aseptic container, 15 μ molm
-2S
-1The blue light illumination of intensity fermentation liquid level, that collects the fermented liquid surface after 6 days becomes orange-yellow mycelium.
Fresh mycelia grinds, and the 1g fresh mycelia adds 18mL alcohol vibration 2h and extracts carotenoid, and 8, the centrifugal 5min of 000r/m gets supernatant liquor, and alcohol is removed in 50 ℃ of distillations, and carotenoid is slightly carried in being of staying.
Claims (8)
1, a kind of from Cordyceps militaris (L.) Link. the technology of fermentative production carotenoid, it is characterized in that: may further comprise the steps: at first Cordyceps militaris (L.) Link. is carried out submerged fermentation, to contain the mycelial fermented liquid of Cordyceps militaris (L.) Link. again places in the container, fermented liquid is carried out illumination, adopt the rayed of 360～470nm wavelength, intensity of illumination is 2～40 μ molm
-2S
-1, perhaps, collect the Cordyceps militaris (L.) Link. fungus filament on the fermented liquid liquid level with common light or the fluorescent lamp irradiation of 20～80W; Again the Cordyceps militaris (L.) Link. fungus filament is ground, use the salt acid treatment, 80～100 ℃ of water-baths, the carotenoid in the pure edible ethanol extracting mycelium obtains dissolved carotenoid, and the carotenoid that is dissolved in the alcohol carries out 40～80 ℃ of distillations, removes alcohol, promptly; The alcohol that perhaps will be dissolved with Cordyceps militaris (L.) Link. carotenoid places 0～-30 ℃, and carotenoid is separated out at low temperatures.
2, according to claim 1 from Cordyceps militaris (L.) Link. the technology of fermentative production carotenoid, it is characterized in that: described fermented liquid is prepared by weight by following component: 1～3% sucrose, 1～2.5% Semen Maydis powder, 2～8% soyflour, 0.1～0.5% potassium primary phosphate, fermented liquid include the Mg that concentration is 0.01～0.05mol/L
2+Surplus is a water.
3, according to claim 1 and 2 from Cordyceps militaris (L.) Link. the technology of fermentative production carotenoid, it is characterized in that: the temperature of fermenting process and illumination process all is controlled between 23～30 ℃.
4, according to claim 1 and 2 from Cordyceps militaris (L.) Link. the technology of fermentative production carotenoid, it is characterized in that: fresh mycelia grinds, and the 1g fresh mycelia adds 10mL 3N hydrochloric acid soln, soak 1～2.5h under the room temperature, water-bath 4min in the boiling water, cooling rapidly, 5, the centrifugal 10min of 000r/min, distilled water washing twice adds 10mL alcohol vibration 30min and extracts carotenoid, 5, the centrifugal 15min of 000r/m gets supernatant liquor.
5, according to claim 1 and 2 from Cordyceps militaris (L.) Link. the technology of fermentative production carotenoid, it is characterized in that: Mg
2+Available sal epsom or magnesium chloride obtain.
6, according to claim 1 and 2 from Cordyceps militaris (L.) Link. the technology of fermentative production carotenoid, it is characterized in that: fermented liquid pH6.5～7.5.
7, according to claim 1 and 2 from Cordyceps militaris (L.) Link. the technology of fermentative production carotenoid, it is characterized in that: the temperature of fermenting process and illumination process all is controlled at 25～28 ℃.
8, according to claim 1 and 2 from Cordyceps militaris (L.) Link. the technology of fermentative production carotenoid, it is characterized in that: illumination is after 3～10 days, the fermented liquid top layer can be transformed into orange-yellow subiculum has plunderred, following fermented liquid also can continue illumination, and carries out the mycelium results 2～3 times.
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| CN 200610018266 CN1840685A (en) | 2006-01-18 | 2006-01-18 | Process for fermentation preparation of carotinoid in aweto |
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| CN 200610018266 CN1840685A (en) | 2006-01-18 | 2006-01-18 | Process for fermentation preparation of carotinoid in aweto |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234676A (en) * | 2010-05-05 | 2011-11-09 | 中国科学院微生物研究所 | Method for producing carotinoid through cordyceps solid culture |
| CN104004815A (en) * | 2014-05-29 | 2014-08-27 | 周礼红 | Culture method of high-yield carotenoid cordyceps militaris |
| CN104109404A (en) * | 2014-07-08 | 2014-10-22 | 浙江理工大学 | Extracting and dyeing methods of natural cordyceps militaris dye |
| CN106046845A (en) * | 2016-05-24 | 2016-10-26 | 广州市澳键丰泽生物科技股份有限公司 | Method for preparing Cordyceps militaris flavochrome powder |
| CN107446982A (en) * | 2017-04-28 | 2017-12-08 | 华南农业大学 | A kind of shaker flask stand liquid fermentation method for improving fan's power mushroom carotenoid production |
| CN107475122A (en) * | 2017-07-12 | 2017-12-15 | 华南农业大学 | It is prepared by fan's power bacterium mycoplasma cultural method of a kind of High Yield of Carotenoid and products thereof |
| CN108948785A (en) * | 2018-10-16 | 2018-12-07 | 安徽祥康生物工程有限公司 | The extracting method of pigment in a kind of Cordyceps militaris |
-
2006
- 2006-01-18 CN CN 200610018266 patent/CN1840685A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234676A (en) * | 2010-05-05 | 2011-11-09 | 中国科学院微生物研究所 | Method for producing carotinoid through cordyceps solid culture |
| CN104004815A (en) * | 2014-05-29 | 2014-08-27 | 周礼红 | Culture method of high-yield carotenoid cordyceps militaris |
| CN104109404A (en) * | 2014-07-08 | 2014-10-22 | 浙江理工大学 | Extracting and dyeing methods of natural cordyceps militaris dye |
| CN104109404B (en) * | 2014-07-08 | 2016-09-14 | 浙江理工大学 | The extracting method of a kind of Cordyceps militaris (L.) Link. natural dye and colouring method thereof |
| CN106046845A (en) * | 2016-05-24 | 2016-10-26 | 广州市澳键丰泽生物科技股份有限公司 | Method for preparing Cordyceps militaris flavochrome powder |
| CN107446982A (en) * | 2017-04-28 | 2017-12-08 | 华南农业大学 | A kind of shaker flask stand liquid fermentation method for improving fan's power mushroom carotenoid production |
| CN107475122A (en) * | 2017-07-12 | 2017-12-15 | 华南农业大学 | It is prepared by fan's power bacterium mycoplasma cultural method of a kind of High Yield of Carotenoid and products thereof |
| CN107475122B (en) * | 2017-07-12 | 2021-01-26 | 华南农业大学 | High-carotenoid-yield labyrin mycoplasm culture method and product preparation thereof |
| CN108948785A (en) * | 2018-10-16 | 2018-12-07 | 安徽祥康生物工程有限公司 | The extracting method of pigment in a kind of Cordyceps militaris |
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