CN1818059A - Production of beta-amylase concentrated liquid with high conversion-rate and purity - Google Patents
Production of beta-amylase concentrated liquid with high conversion-rate and purity Download PDFInfo
- Publication number
- CN1818059A CN1818059A CN 200510136657 CN200510136657A CN1818059A CN 1818059 A CN1818059 A CN 1818059A CN 200510136657 CN200510136657 CN 200510136657 CN 200510136657 A CN200510136657 A CN 200510136657A CN 1818059 A CN1818059 A CN 1818059A
- Authority
- CN
- China
- Prior art keywords
- jar
- raw material
- fermentation
- seed
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A production method of the high percent conversion and purity saccharifying enzyme concentrated solution includes: loading the carbon source, corn plasm, ammonium sulfate and the water as the match into the first seeding tank; loading the carbon source, bean cake powder, the liquid ammonia, alpha-amylase, the biotin and the water as the match into the secondary seeding tank; loading the sodium bentonite and the acidizing water as the match into the purifying pretreatment tank; inoculating the Aspergillus niger into the first seeding tank to culture then transferring the cultured strain and the medium into the secondary seeding tank to culture amplifily last to transfer to the fermenter to ferment; next to lead the zymotic fluid in to the pretreatment tank to pretreatment; next to separate, concentrate, filtrate, aseptic and detect to get the enzyme. It can improve the DE value apparently.
Description
Technical field
The present invention relates to a kind of production method that acts on the lytic enzyme on the glycosyl compound, specifically, relate to a kind of production method of beta-amylase concentrated liquid with high conversion-rate and purity.
Background technology
Saccharifying enzyme is as a kind of biological catalyst, be mainly used in the saccharifying enzyme process (promptly being that starch is converted into glucose) of industry starch such as food fermentation, medicine, its action principle is: saccharifying enzyme acts on α-1.4 glycosidic link of starch, starch is hydrolyzed to glucose one by one, as be applied at first starchy material is hydrolyzed to glucose in the alcohol industry by saccharifying enzyme.By yeast fermentation, be ethanol (alcohol) and carbonic acid gas with conversion of glucose, thereby finish the production of alcohol then.And the problem that the saccharifying enzyme of China's production at present mainly exists is: because it is unreasonable to produce the fermentation and the purifying process of saccharifying enzyme, make that saccharifying enzyme finished product enzyme is impure, particularly contain more α-Dian Fenmei and glucose transglucosidase (generally contain α-Dian Fenmei 200u/ml, more than the glucose transglucosidase 100u/ml) in the finished product, α-Dian Fenmei is present in the saccharifying enzyme finished product, influence the accuracy that saccharifying enzymic activity is measured, it is accurately quantitative that thereby influence is used, and Mashing process is had a negative impact; The glucose transglucosidase is non-fermentable panose and isomaltose with conversion of glucose in application process, the yield of affecting glucose.The existing saccharifying enzyme of producing is owing to exist above deficiency, therefore further makes DE value (being inversion rate of glucose) generally have only about 95% and be difficult to raising.
Summary of the invention
The saccharifying enzyme finished product enzyme that exists for the production technique that solves existing saccharifying enzyme be impure and and then have influence on the not high enough problem of inversion rate of glucose (DE value) when using, purpose of the present invention, it is the production method that improves a kind of beta-amylase concentrated liquid with high conversion-rate and purity, thereby reduce the content of α-Dian Fenmei and glucose transglucosidase in the saccharifying enzyme finished product, and then the inversion rate of glucose when improving the saccharifying enzyme finished product and using.
Solution of the present invention is as follows: (a) press column weight amount proportioning and pack into and be used for the raw material of seed culture in first class seed pot: 8～17% carbon is former, 1～3% corn steep liquor, 1～2% ammonium sulfate, 80%～88% water; (b) pressing column weight amount proportioning in the secondary seed jar packs into and be used for the raw material of seed culture: 8～17% carbon is former, 2～4% soybean cake powder, 0.01～0.015% α-Dian Fenmei, 0.008～0.01% vitamin H, 80%～88% water; And the total raw material that secondary seed jar and first class seed pot are used for seed culture is configured by 78%～88% to 12～22% part by weight respectively; (c) pressing column weight amount proportioning in fermentor tank packs into and be used for the raw material that ferments after the seed culture: 15～27% carbon is former, 3～7% soybean cake powder, 0.3～0.5% ammonium sulfate, the liquefied ammonia that 2～7% streams add, 0.025～0.05% α-Dian Fenmei, 0.005～0.01% vitamin H, 62%～75% water; And the total raw material that total raw material that fermentor tank is used to ferment and secondary seed jar are used for seed culture is configured by 82～90% to 10～18% part by weight respectively; (d) pressing column weight amount proportioning in purifying pre-treatment jar packs into and is used for carrying out the pretreated raw material of purifying after fermentation: 2～5% sodium bentonite, 95%～98% the water after acidification; (e) press column weight amount proportioning in the first class seed pot of seed culture raw material of packing into and insert aspergillus niger strain: 0.05～0.6% aspergillus niger strain, 99.4～99.95% first class seed pot are used for the total raw material weight of seed culture; Culture temperature is 34 ℃ ± 1 ℃ in the first class seed pot, and pressure is 0.08～0.1Mpa, and air flow is 1: 1～1.2, and the pH value that just ferments is 4.8～5.0, and incubation time is 25～40hr; (f) will cultivate sophisticated first class seed pot strain liquid moves to and carries out enlarged culturing in the secondary seed jar, culture temperature is 34 ℃ ± 1 ℃, the jar internal pressure is 0.05～0.08Mpa, air flow is 1: 0.7～1, just the fermentation pH value is 4.8～5.0, be 4.7 ± 1 with the method control pH value that stream adds liquefied ammonia mid-term, and incubation time is 35～50hr; (g) carry out fermentation culture with cultivating in the secondary seed jar in the fermentor tank that sophisticated strain liquid moves to the fermentation raw material of packing into, leavening temperature is 34 ℃ ± 1 ℃, the jar internal pressure is 0.05～0.07Mpa, air flow is 1: 0.5～1, just the fermentation pH value is 4.8～5.0, when pH value drops to 4.5 left and right sides, the method control pH value that adds liquefied ammonia with stream is 4.7 ± 1, in the fermenting process, when the DE value drops to 25-30,, add the sterilization syrup and carry out feed supplement with the consumption of 2.5～1000L per hour, keep the rational carbon of fermented liquid during the fermentation, nitrogen ratio and osmotic pressure, fermentation time is 135～170hr; (h) after fermentation is finished maturing fermentation liquid is put into the purifying pre-treatment jar that installs pretreating raw material and carry out the purifying pre-treatment, treatment temp is 20 ℃～30 ℃, pH value is 2.8～3.2, treatment time is 1.5～3hr, fermented liquid carries out solid-liquid separation by plate-and-frame filter press after pre-treatment, the enzyme liquid after the separation carries out ultrafiltration and concentration again, and the enzyme liquid after concentrating carries out smart filtering bacterium, enzyme liquid behind the smart filtering bacterium is allocated after the physics and chemistry detection is qualified, and enzyme gets product.
The beneficial effect of the inventive method is, can increase substantially fermentation level of glucoamylase, and saccharifying enzyme finished product enzyme is the purity height, can obviously improve DE value (inversion rate of glucose); In addition,, can improve plant factor owing to adopt pulse fed-batch fermentation technology, save energy, by after the purifying pre-treatment, waste discharge contamination index significantly reduces, and helps environment protection in the purifying leaching process.The saccharifying enzyme that utilizes this production method to produce, the key technical indexes reaches international like product advanced level, and its basic mechanical design feature index is as follows: saccharifying enzymic activity: 150000u/ml; α-Dian Fenmeihanliang: do not detect (promptly being essentially 0u/ml); Glucose transglucosidase content :≤10u/ml; Total plate count:＜100/ml; DE value (inversion rate of glucose):＞98%.
Embodiment
Embodiment one: first class seed pot adopts 1M
3Jar, constant volume 0.8, in jar by gross weight 800kg batching, the former 80kg of carbon containing wherein, corn steep liquor 8kg, ammonium sulfate 8kg, water 704kg; The secondary seed jar adopts 5M
3Jar, constant volume 0.8, prepare burden by gross weight 4000kg in jar, the former 400kg of carbon containing wherein, soybean cake powder 80kg, α-Dian Fenmei 0.4kg, vitamin H 0.32kg, water 3519.28kg, so secondary seed jar and the first class seed pot total raw material that is used for seed culture is that (be 4000kg: 800kg=5: part by weight 1) is configured by 83.3% to 16.7% respectively; Fermentor tank adopts 30M
3Jar, constant volume 0.9, in jar by gross weight 27000kg batching, the former 4960Kg of carbon containing wherein, soybean cake powder 1080Kg, ammonium sulfate 100kg, stream adds liquefied ammonia 880Kg, α-Dian Fenmei 8.1kg, vitamin H 1.62Kg, water 19970.28kg, so the fermentor tank total raw material that is used to ferment and the secondary seed jar total raw material that is used for seed culture is that (be 27000Kg: 4000kg=6.7: part by weight 1) is configured by 87% to 13% respectively; The sodium bentonite 940kg that packs in the purifying pre-treatment jar, the water 22050kg after acidification; Insert aspergillus niger strain 0.5kg in the first class seed pot of seed culture raw material of packing into, inoculation temp is 33.5 ℃, and culture temperature is 33.5 ℃, tank pressure is 0.08Mpa, air flow is 1: 1, and pH value is 4.8, moves into the secondary seed jar behind the cultivation 30hr and carries out enlarged culturing; Culture temperature is 33.5 ℃ in the secondary seed jar, and tank pressure is 0.06Mpa, and air flow is 1: 0.8, and pH value is 4.8, moves into fermentor tank behind the cultivation 40hr and ferments; Leavening temperature in the fermentor tank is 33.5 ℃, tank pressure is 0.05Mpa, air flow is 1: 0.6, pH value is 4.7 ± 1, when the DE value drops to 25～30, presses per hour 5～5001 consumption in the fermenting process, add the sterilization syrup and carry out feed supplement, it is 4.7 ± 1 that stream adds liquefied ammonia control pH value, fermentation 142hr, and putting enzyme activity is 51112u/ml; After fermentation is finished maturing fermentation liquid is put into the purifying pre-treatment jar that installs pretreating raw material and carry out the purifying pre-treatment, treatment temp is 28 ℃, pH value is 2.0, carry out solid-liquid separation by plate-and-frame filter press after handling 1.5hr, separation temperature is 26 ℃, after the 10hr separation finishes enzyme liquid is carried out ultrafiltration and concentration, thickening temperature is 26 ℃, carries out smart filtering bacterium after 12hr concentrates, smart filter temperature is 26 ℃, finish through 3hr, get smart filtrate and carry out physical and chemical testing, allocate after qualified, the finished product enzyme activity is 151000u/ml, transglucosidase content is 7u/ml, and α-Dian Fenmei does not detect (being ou/ml), and total plate count is 76/ml.
Embodiment two: first class seed pot adopts 1M
3Jar, constant volume 0.8, in jar by gross weight 800kg batching, the former 112kg of carbon containing wherein, ammonium sulfate 16kg, corn steep liquor 12kg, water 660kg; The secondary seed jar adopts 5M
3Jar, constant volume 0.8, prepare burden by gross weight 4000kg in jar, the former 540kg of carbon containing wherein, soybean cake powder 140kg, α-Dian Fenmei 0.52kg, vitamin H 0.4kg, water 3319.08kg, so secondary seed jar and the first class seed pot total raw material that is used for seed culture is that (be 4000kg: 800kg=5: part by weight 1) is configured by 83.3% to 16.7% respectively; Fermentor tank adopts 35M
3Jar, constant volume 0.9, in jar by gross weight 31500kg batching, the former 8505kg of carbon containing wherein, soybean cake powder 1260kg, ammonium sulfate 150Kg, stream adds liquefied ammonia 970kg, α-Dian Fenmei 12.6kg, vitamin H 2.835kg, water 20599.565kg, so the fermentor tank total raw material that is used to ferment and the secondary seed jar total raw material that is used for seed culture is that (be 31500kg: 4000kg=7.87: part by weight 1) is configured by 88.73% to 11.27% respectively; The sodium bentonite 1380kg that packs in the purifying pre-treatment jar, the water 33000kg after acidification; Insert aspergillus niger strain 0.5kg in the first class seed pot of seed culture raw material of packing into, inoculation temp is 34.5 ℃, and culture temperature is 34.5 ℃, tank pressure is 0.1Mpa, air flow is 1: 1.18, and initial p H value is 4.9, moves into the secondary seed jar behind the cultivation 43hr and carries out enlarged culturing; Culture temperature is 34.5 ℃ in the secondary seed jar, and tank pressure is 0.08Mpa, and air flow is 1: 1, and initial p H value is 4.9, moves into fermentor tank behind the cultivation 48hr and ferments; Leavening temperature in the fermentor tank is 34.5 ℃, tank pressure is 0.07Mpa, air flow is 1: 0.9, initial p H value is 4.9, in the fermenting process, when the DE value drops to 25～30, add the sterilization syrup by the consumption of 15～900L per hour and carry out feed supplement, it is 4.7 ± 0.1 that stream adds liquefied ammonia control pH value, fermentation 169hr, and putting jar enzyme activity is 54690u/ml; After fermentation is finished maturing fermentation liquid is put into the purifying pre-treatment jar that installs pretreating raw material and carry out the purifying pre-treatment, treatment temp is 30 ℃, pH value is 3.0, carry out solid-liquid separation by plate-and-frame filter press after handling 2hr, separation temperature is 27 ℃, after the 12hr separation finishes enzyme liquid is carried out ultrafiltration and concentration, thickening temperature is 27 ℃, carries out smart filtering bacterium after 14hr concentrates, smart filter temperature is 27 ℃, finish through 3.5hr, get smart filtrate and carry out physical and chemical testing, allocate after qualified, the finished product enzyme activity is 150900u/ml, transglucosidase content is 5u/ml, and α-Dian Fenmei does not detect, and total plate count is 81/ml.
Embodiment three: first class seed pot adopts 1M
3Jar, constant volume 0.8, in jar by gross weight 800kg batching, the former 104kg of carbon containing wherein, corn steep liquor 12kg, ammonium sulfate 12kg, water 672kg; The secondary seed jar adopts 5M
3Jar, constant volume 0.8, prepare burden by gross weight 4000kg in jar, the former 480kg of carbon containing wherein, soybean cake powder 120kg, α-Dian Fenmei 0.6kg, vitamin H 0.36kg, water 3399.04kg, so secondary seed jar and the first class seed pot total raw material that is used for seed culture is that (be 4000kg: 800kg=5: 1 part by weight is configured by 83.3% to 16.7% respectively; Fermentor tank adopts 38M
3Jar, constant volume 0.9, in jar by gross weight 34000kg batching, the former 8840kg of carbon containing wherein, soybean cake powder 1310kg, ammonium sulfate 150Kg, stream adds liquefied ammonia 920kg, α-Dian Fenmei 12.5kg, vitamin H 2.72kg, water 22764.78kg, so the fermentor tank total raw material that is used to ferment and the secondary seed jar total raw material that is used for seed culture is that (be 34000kg: 4000kg=8.5: part by weight 1) is configured by 89.5 to 10.5% respectively; The sodium bentonite 1010kg that packs in the purifying pre-treatment jar, the water 43200kg after acidification; Insert aspergillus niger strain 0.5kg in the first class seed pot of seed culture raw material of packing into, inoculation temp is 34 ℃, and culture temperature is 34 ℃, tank pressure is 0.09Mpa, air flow is 1: 1.15, and initial p H value is 4.8, moves into the secondary seed jar behind the cultivation 40hr and carries out enlarged culturing; Culture temperature is 34 ℃ in the secondary seed jar, and tank pressure is 0.07Mpa, and air flow is 1: 0.9, and initial p H value is 4.8, moves into fermentor tank behind the cultivation 46hr and ferments; Leavening temperature in the fermentor tank is 34 ℃, and tank pressure is 0.06Mpa, and air flow is 1: 0.8, initial p H value is 4.8, in the fermenting process, when the DE value drops to 25～30, press the per hour consumption of 10～800L, add the sterilization syrup and carry out feed supplement, it is 4.7 ± 0.1 that stream adds liquefied ammonia control pH value.Fermentation 158hr, putting enzyme activity is 58000u/ml; After fermentation is finished maturing fermentation liquid is put into the purifying pre-treatment jar that installs pretreating raw material and carry out the purifying pre-treatment, treatment temp is 25 ℃, pH value is 4.0, carry out solid-liquid separation by plate-and-frame filter press after handling 2.5hr, separation temperature is 23 ℃, after the 12.5hr separation finishes enzyme liquid is carried out ultrafiltration and concentration, thickening temperature is 23 ℃, carries out smart filtering bacterium after 14hr concentrates, smart filter temperature is 27 ℃, finish through 3.8hr, get smart filtrate and carry out physical and chemical testing, allocate after qualified, the finished product enzyme activity is 15100u/ml, transglucosidase content is 4u/ml, and the α-Dian Fenmei end detects, and total plate count is 81/ml.
In the above-described embodiments, the former W-Gum that adopts of carbon also can adopt Semen Maydis powder.First class seed pot, secondary seed jar, fermentation tank culture medium and the syrupy sterilising temp of feed supplement are 121～123 ℃, and sterilization time is 45 minutes.
Claims (3)
1, a kind of production method of beta-amylase concentrated liquid with high conversion-rate and purity, it is characterized in that: (a) press column weight amount proportioning and pack into and be used for the raw material of seed culture in first class seed pot: 8～17% carbon is former, 1～3% corn steep liquor, 1～2% ammonium sulfate, 80%～88% water; (b) pressing column weight amount proportioning in the secondary seed jar packs into and be used for the raw material of seed culture: 8～17% carbon is former, 2～4% soybean cake powder, 0.01～0.015% α-Dian Fenmei, 0.008～0.01% vitamin H, 80%～88% water; And the total raw material that secondary seed jar and first class seed pot are used for seed culture is configured by 78%～88% to 12～22% part by weight respectively; (c) pressing column weight amount proportioning in fermentor tank packs into and be used for the raw material that ferments after the seed culture: 15～27% carbon is former, 3～7% soybean cake powder, 0.3～0.5% ammonium sulfate, the liquefied ammonia that 2～7% streams add, 0.025～0.05% α-Dian Fenmei, 0.005～0.01% vitamin H, 62%～75% water; And the total raw material that total raw material that fermentor tank is used to ferment and secondary seed jar are used for seed culture is configured by 82～90% to 10～18% part by weight respectively; (d) pressing column weight amount proportioning in purifying pre-treatment jar packs into and is used for carrying out the pretreated raw material of purifying after fermentation: 2～5% sodium bentonite, 95%～98% the water after acidification; (e) press column weight amount proportioning in the first class seed pot of seed culture raw material of packing into and insert aspergillus niger strain: 0.05～0.6% aspergillus niger strain, 99.4～99.95% first class seed pot are used for the total raw material weight of seed culture; Culture temperature is 34 ℃ ± 1 ℃ in the first class seed pot, and pressure is 0.08～0.1Mpa, and air flow is 1: 1～1.2, and the pH value that just ferments is 4.8～5.0, and incubation time is 25～40hr; (f) will cultivate sophisticated first class seed pot strain liquid moves to and carries out enlarged culturing in the secondary seed jar, culture temperature is 34 ℃ ± 1 ℃, the jar internal pressure is 0.05～0.08Mpa, air flow is 1: 0.7～1, just the fermentation pH value is 4.8～5.0, be 4.7 ± 1 with the method control pH value that stream adds liquefied ammonia mid-term, and incubation time is 35～50hr; (g) carry out fermentation culture with cultivating in the secondary seed jar in the fermentor tank that sophisticated strain liquid moves to the fermentation raw material of packing into, leavening temperature is 34 ℃ ± 1 ℃, the jar internal pressure is 0.05～0.07Mpa, air flow is 1: 0.5～1, just the fermentation pH value is 4.8～5.0, when pH value drops to 4.5 left and right sides, the method control pH value that adds liquefied ammonia with stream is 4.7 ± 1, in the fermenting process, when the DE value drops to 25-30,, add the sterilization syrup and carry out feed supplement with the consumption of 2.5～1000L per hour, keep the rational carbon of fermented liquid during the fermentation, nitrogen ratio and osmotic pressure, fermentation time is 135～170hr; (h) after fermentation is finished maturing fermentation liquid is put into the purifying pre-treatment jar that installs pretreating raw material and carry out the purifying pre-treatment, treatment temp is 20 ℃～30 ℃, pH value is 2.8～3.2, treatment time is 1.5～3hr, fermented liquid carries out solid-liquid separation by plate-and-frame filter press after pre-treatment, the enzyme liquid after the separation carries out ultrafiltration and concentration again, and the enzyme liquid after concentrating carries out smart filtering bacterium, enzyme liquid behind the smart filtering bacterium is allocated after the physics and chemistry detection is qualified, and enzyme gets product.
2, the production method of beta-amylase concentrated liquid with high conversion-rate and purity according to claim 1 is characterized in that, the former employing W-Gum of described carbon.
3, the production method of beta-amylase concentrated liquid with high conversion-rate and purity according to claim 1 is characterized in that, the former employing Semen Maydis powder of described carbon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101366571A CN100443589C (en) | 2005-12-30 | 2005-12-30 | Production of beta-amylase concentrated liquid with high conversion-rate and purity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101366571A CN100443589C (en) | 2005-12-30 | 2005-12-30 | Production of beta-amylase concentrated liquid with high conversion-rate and purity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1818059A true CN1818059A (en) | 2006-08-16 |
| CN100443589C CN100443589C (en) | 2008-12-17 |
Family
ID=36918258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101366571A Expired - Fee Related CN100443589C (en) | 2005-12-30 | 2005-12-30 | Production of beta-amylase concentrated liquid with high conversion-rate and purity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100443589C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100427583C (en) * | 2006-10-20 | 2008-10-22 | 山西大学 | Active strain of high-activity saccharifying enzyme, enzyme preparation, and their preparation method and use |
| CN101570725B (en) * | 2009-06-10 | 2011-12-14 | 山西三盟实业发展有限公司 | Method for starter propagation by utilizing smoked vinegar lees |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210888A (en) * | 1998-10-05 | 1999-03-17 | 清华大学 | Process for producing saccharifying enzyme using waste molasses as main raw material |
| WO2001004273A2 (en) * | 1999-07-09 | 2001-01-18 | Novozymes A/S | Glucoamylase variant |
-
2005
- 2005-12-30 CN CNB2005101366571A patent/CN100443589C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100427583C (en) * | 2006-10-20 | 2008-10-22 | 山西大学 | Active strain of high-activity saccharifying enzyme, enzyme preparation, and their preparation method and use |
| CN101570725B (en) * | 2009-06-10 | 2011-12-14 | 山西三盟实业发展有限公司 | Method for starter propagation by utilizing smoked vinegar lees |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100443589C (en) | 2008-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102533889B (en) | Method for continuously fermenting lysine | |
| CN110846362B (en) | Neomycin sulfate fermentation clean production method | |
| CN107058200A (en) | The method for preparing the glucoside of L ascorbic acid 2 | |
| CN102533877B (en) | Method for preparing citric acid by fermentation | |
| CN111040982A (en) | Saccharomyces cerevisiae promoter and preparation method and application process thereof | |
| CN102329718B (en) | Method for preparing vinegar by continuously fermenting multi-strain immobilized cell composition | |
| CN104561140B (en) | A kind of method of preparation of citric acid by fermentation | |
| CN100443589C (en) | Production of beta-amylase concentrated liquid with high conversion-rate and purity | |
| CN103146769B (en) | Method for preparating citric acid by fermentation | |
| CN102373242B (en) | Citric acid preparation method | |
| CN101638674B (en) | Method for manufacturing citric acid by utilizing cane sugar hydrolysate fermentation method | |
| CN101638675B (en) | Method for manufacturing citric acid by cane sugar fermentation method | |
| CN110157603A (en) | A kind of Immobilized Aspergillus niger produces the fermentation reactor and its method of citric acid | |
| CN110564804B (en) | Clear liquid fermentation medium for producing riboflavin and fermentation method | |
| CN106957882B (en) | Method for preparing citric acid by fermentation | |
| CN102260716B (en) | Fermentation broth for citric acid fermentation and fermentation method using same | |
| CN111172204B (en) | Preparation method for improving citric acid fermentation efficiency | |
| CN101974500A (en) | Production method of high-purity and intermediate-temperate alpha-amylase | |
| CN102776166B (en) | Production method for xylanase | |
| CN202968544U (en) | Continuous fermentation propagation device for reducing contamination probability | |
| CN102321685A (en) | A kind of preparation method of citric acid fermentation broth | |
| CN103820419B (en) | A kind of preparation method of the good beta glucan enzyme of heat endurance | |
| CN106754829A (en) | A kind of method of utilization bacillus HS17 fermenting and producing chitosan enzymes and its application | |
| CN112852896A (en) | Fermentation production method of L-arginine | |
| CN85104280A (en) | A kind of method of utilizing microorganism continuous brewing water fruit vinegar |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: HUNAN HONGYING BIOLOGICAL SCIENCE + TECHNOLOGY CO. Free format text: FORMER OWNER: HONGYINGXIANG BIOLOGICAL ENGINEERING CO., LTD., HUNAN Effective date: 20140627 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20140627 Address after: Lu Hongying Industrial District Tianjin 415400 city in Hunan province Changde City No. 10 Patentee after: Hunan Hongying Biological Science & Technology Co., Ltd. Address before: 415400 No. 362 North Main Road, Tianjin city, Hunan Patentee before: Hongyingxiang Biological Engineering Co., Ltd., Hunan |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081217 Termination date: 20181230 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |