CN1810952A - Microbe fermenting culture liquid and its prepn process - Google Patents
Microbe fermenting culture liquid and its prepn process Download PDFInfo
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- CN1810952A CN1810952A CN 200610038422 CN200610038422A CN1810952A CN 1810952 A CN1810952 A CN 1810952A CN 200610038422 CN200610038422 CN 200610038422 CN 200610038422 A CN200610038422 A CN 200610038422A CN 1810952 A CN1810952 A CN 1810952A
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- phytophthora
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Abstract
The present invention discloses one kind of microbe fermenting culture liquid and its preparation process. Tomato, carrot, cucumber, lettuce and celery in certain weight proportion are squeezed separately to obtain juice, and the five kinds of juice are mixed and high pressure steam sterilized at 121 deg.c for 15 min. The mixed juice is further compounded into special phytophthora fermenting culture liquid or medium. The present invention alters traditional phytophthora culturing technology, has greatly lowered phytophthora fermenting culture cost, and may be used widely in agriculture, biomedicine industry, fermentation industry, and food and beverage industry.
Description
Technical field
The present invention relates to a kind of microbe fermenting culture liquid and manufacture craft thereof.
Background technology
Phytophthora microbial fermentation abroad or nutrient solution (base) be much more general adopt that U.S. Campbell company produces by eight kinds of vegetables be the formulated pop can canned drink of main component as mother liquor, the formulated phytophthora fermenting culture liquid in certain proportion dilution back (is called for short V
8Nutrient solution), nutrient solution can be made phytophthora special culture media (abbreviation V after adding the agar peptizer
8Substratum).But because V
8Fermentation culture (base) is difficult to buy in China, must be from U.S.'s import, and prices are rather stiff, and every tin of 250 milliliters of mother liquors need about about 20 yuan～25 yuan of Renminbi, and must be from U.S.'s import, and are of a high price.In recent years, the someone proposes (to be called for short V with the beverage (the happy dish of commodity dimension by name) that is hybridly prepared into by 6 kinds of vegetables juice that Beijing Agricultural University produces for the formulated phytophthora fermenting culture liquid of main component
6Nutrient solution), nutrient solution can be made phytophthora special culture media (abbreviation V after adding the agar peptizer
6Substratum), still for various reasons, the happy dish of dimension that is hybridly prepared into by 6 kinds of vegetables juice that Beijing Agricultural University produces stops production at present.
At present, fermentation culture to phytophthora does not still have good fermenting culture liquid (base), main at present employing 6% lima bean flour water culture and 30% oatmeal solution are cultivated, and cost is higher, and the mycelia amount that cultivation produces is fewer, effect is poor.The phytophthora fermentation culture does not still have the fermented liquid of phytophthora special use at present in biological-pharmacy and fermentation industry.
Summary of the invention
The objective of the invention is: a kind of microbe fermenting culture liquid and manufacture craft thereof are provided, this process modification traditional phytophthora culture technique, reduced phytophthora significantly and cultivated or fermentation costs, the various phytophthoras that can be widely used in agricultural, biological-pharmacy, fermentation industry and food_beverage industry are cultivated and fermentation.
Technical scheme of the present invention is: a kind of microbe fermenting culture liquid, and by the juice of tomato, Radix Dauci Sativae, cucumber, lettuce, 5 kinds of vegetables of celery, 9: 9: 3 by volume: mix at 3: 1.
A kind of manufacture craft of microbe fermenting culture liquid, first-selected with tomato, Radix Dauci Sativae, cucumber, lettuce, 5 kinds of vegetables wash respectively celery, shred after drying the globule that adheres to, place and put into the freeze thawing of subzero 20 ℃ of refrigerator refrigerators in the container, take out after 48 hours to place and thaw naturally in the container or the airtight defreezing by microwave oven of then using of container, treat with tissue mashing machine 5 kinds of vegetables to be smashed to pieces respectively after thawing is thawed, filter and extract with double-deck hospital gauze again, 9: 9: 3 by volume: measure juice mixing, through through 121 ℃ high pressure steam sterilizations 15 minute handle after promptly can be made into mother liquor at 3: 1.
Mother liquor can be mixed with 10% concentration with sterilized water or tap water, handle in 15 minutes through 121 ℃ of high pressure steam sterilizations again, can be mixed with the phytophthora fermenting culture liquid.
After adding 2% agar peptizer according to part by weight in can the nutrient solution before sterilization, make the phytophthora special culture media.
Advantage of the present invention is:
1. the present invention has improved traditional phytophthora culture technique, has reduced phytophthora significantly and has cultivated or fermentation costs, and the various phytophthoras that can be widely used in agricultural, biological-pharmacy, fermentation industry and food_beverage industry are cultivated and fermentation.
2. the present invention compares with fermentation culture at present commonly used, and raw material conveniently is easy to get, low price, cultivation produce mycelia and sporocyst effect are remarkable, are specially adapted to phytophthora mycelia and sporangial a large amount of cultivation.
The invention will be further described below in conjunction with embodiment:
Embodiment
Embodiment: a kind of microbe fermenting culture liquid, adopt tomato, Radix Dauci Sativae, cucumber, lettuce, 5 kinds of vegetables of celery, squeeze vegetables juice according to certain technical process, mother liquor is according to tomato, Radix Dauci Sativae, cucumber, lettuce, the volume ratio of 5 kinds of vegetables juice of celery 9: 9: 3: after 3: 1 the mixed, make through 121 ℃ of high pressure steam sterilizations 15 minutes, during use, mother liquor can be mixed with 10% concentration as required with sterilized water or tap water, handled in 15 minutes through 121 ℃ of high pressure steam sterilizations again, can be mixed with the phytophthora fermenting culture liquid, if after adding 2% agar peptizer according to part by weight in the nutrient solution before sterilization, can make the phytophthora special culture media.
A kind of manufacture craft of microbe fermenting culture liquid, first-selected that 5 kinds of vegetables are clean with tap water respectively, shred after drying the globule that adheres to, place and put into the freeze thawing of subzero 20 ℃ of refrigerator refrigerators in the container, take out after 48 hours to place and thaw naturally in the container or the airtight defreezing by microwave oven of then using of container, treat with tissue mashing machine 5 kinds of vegetables to be smashed to pieces respectively after thawing is thawed, filter and extract with double-deck hospital gauze again, by volume ratio is measured the juice mixing, after handling in 15 minutes, promptly can be made into mother liquor through 121 ℃ of high pressure steam sterilizations, during use, mother liquor can be mixed with 10% concentration as required with sterilized water or tap water, handled in 15 minutes through 121 ℃ of high pressure steam sterilizations again, can be mixed with the phytophthora fermenting culture liquid, if after adding 2% agar peptizer according to part by weight in the nutrient solution before sterilization, can make the phytophthora special culture media.
The present invention has improved traditional phytophthora culture technique, has reduced phytophthora significantly and has cultivated or fermentation costs, and the various phytophthoras that can be widely used in agricultural, biological-pharmacy, fermentation industry and food_beverage industry are cultivated and fermentation.
The present invention compares with fermentation culture at present commonly used, and raw material conveniently is easy to get, low price, cultivation produce mycelia and sporocyst effect are remarkable, are specially adapted to phytophthora mycelia and sporangial a large amount of cultivation.
Claims (4)
1. a microbe fermenting culture liquid is characterized in that: by the juice of tomato, Radix Dauci Sativae, cucumber, lettuce, 5 kinds of vegetables of celery, 9: 9: 3 by volume: mix at 3: 1.
2. the manufacture craft of a microbe fermenting culture liquid, it is characterized in that: first-selected tomato, Radix Dauci Sativae, cucumber, lettuce, 5 kinds of vegetables wash respectively celery, shred after drying the globule that adheres to, place and put into the freeze thawing of subzero 20 ℃ of refrigerator refrigerators in the container, take out after 48 hours to place and thaw naturally in the container or the airtight defreezing by microwave oven of then using of container, treat with tissue mashing machine 5 kinds of vegetables to be smashed to pieces respectively after thawing is thawed, filter and extract with double-deck hospital gauze again, 9: 9: 3 by volume: measure juice mixing at 3: 1, promptly can be made into mother liquor after handling in 15 minutes through 121 ℃ of high pressure steam sterilizations.
3. the manufacture craft of microbe fermenting culture liquid according to claim 2, it is characterized in that: mother liquor can be mixed with 10% concentration with sterilized water or tap water, handled in 15 minutes through 121 ℃ of high pressure steam sterilizations again, can be mixed with the phytophthora fermenting culture liquid.
4. the manufacture craft of microbe fermenting culture liquid according to claim 2 is characterized in that: after adding 2% agar peptizer according to part by weight in can the nutrient solution before sterilization, make the phytophthora special culture media.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100384223A CN100408671C (en) | 2006-02-16 | 2006-02-16 | Microbe fermenting culture liquid and its prepn process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100384223A CN100408671C (en) | 2006-02-16 | 2006-02-16 | Microbe fermenting culture liquid and its prepn process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1810952A true CN1810952A (en) | 2006-08-02 |
| CN100408671C CN100408671C (en) | 2008-08-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100384223A Expired - Fee Related CN100408671C (en) | 2006-02-16 | 2006-02-16 | Microbe fermenting culture liquid and its prepn process |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985604A (en) * | 2010-12-10 | 2011-03-16 | 福建省农业科学院植物保护研究所 | Method for efficiently separating Phytophthora capsici from aging disease tissue |
| CN102643776A (en) * | 2012-03-31 | 2012-08-22 | 云南农业大学 | Method for preparing culture medium appropriate for phytophthotacactorum to produce sporangiums by taking radish as raw material |
| CN101380127B (en) * | 2007-09-08 | 2012-11-21 | 师瑞彬 | Preparation method of beverage using wild plant as main material |
| CN102839148A (en) * | 2012-08-28 | 2012-12-26 | 云南农业大学 | Fluid medium made from celery and suitable for phytophthotacactorum to generate sporangium |
| CN102839147A (en) * | 2012-08-28 | 2012-12-26 | 云南农业大学 | Fluid medium made from pseudo-ginseng and suitable for phytophthotacactorum to generate zoospore |
-
2006
- 2006-02-16 CN CNB2006100384223A patent/CN100408671C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101380127B (en) * | 2007-09-08 | 2012-11-21 | 师瑞彬 | Preparation method of beverage using wild plant as main material |
| CN101985604A (en) * | 2010-12-10 | 2011-03-16 | 福建省农业科学院植物保护研究所 | Method for efficiently separating Phytophthora capsici from aging disease tissue |
| CN102643776A (en) * | 2012-03-31 | 2012-08-22 | 云南农业大学 | Method for preparing culture medium appropriate for phytophthotacactorum to produce sporangiums by taking radish as raw material |
| CN102643776B (en) * | 2012-03-31 | 2013-09-04 | 云南农业大学 | Culture medium appropriate for phytophthotacactorum to produce sporangiums by taking radish as raw material |
| CN102839148A (en) * | 2012-08-28 | 2012-12-26 | 云南农业大学 | Fluid medium made from celery and suitable for phytophthotacactorum to generate sporangium |
| CN102839147A (en) * | 2012-08-28 | 2012-12-26 | 云南农业大学 | Fluid medium made from pseudo-ginseng and suitable for phytophthotacactorum to generate zoospore |
| CN102839147B (en) * | 2012-08-28 | 2014-12-17 | 云南农业大学 | Fluid medium made from pseudo-ginseng and suitable for phytophthotacactorum to generate zoospore |
| CN102839148B (en) * | 2012-08-28 | 2014-12-17 | 云南农业大学 | Fluid medium made from celery and suitable for phytophthotacactorum to generate sporangium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100408671C (en) | 2008-08-06 |
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Granted publication date: 20080806 Termination date: 20130216 |