CN1804039A - Virulence genes, proteins, and their use - Google Patents

Abstract

A series of genes from Streptococcus pyogenes are shown to encode products which are implicated in virulence. The identification of these genes therefore allows attenuated microorganisms to be produced. Furthermore, the genes or their encoded products can be used in the manufacture of vaccines for therapeutic application.

Description

Virulence gene, protein and uses thereof

The application's dividing an application that be the denomination of invention submitted on December 22nd, 2000 for the Chinese invention patent application No.00818356.2 of " virulence gene, protein and uses thereof ".

Invention field

The present invention relates to virulence gene and protein, and uses thereof.More particularly, the present invention relates to from the gene and the proteins/peptides of streptococcus pyogenes (Streptococcus pyogenes) acquisition, and the purposes aspect treatment and screening of medicaments.

Background of invention

A group streptococcus (GAS) is the cause of disease of most of suis diseases.Making us interested a kind of biology especially is streptococcus pyogenes, and it relates to a lot of Non-Invasives and aggressive infects, for example purulence kitchen sore, pharyngitis, necrotizing fasciitis, microbemia, suis toxic shock syndrome (STSS), pneumonia and rheumatic fever.

Some GAS infect can use antibiotic therapy, comprises penicillin and erythromycin.Yet, because the problem relevant with the microbiotic allergy patient with antibiotics resistance need can be used for the therapy for the treatment of or preventing GAS to infect in addition.

Summary of the invention

The present invention is based on the discovery of virulence gene in the streptococcus pyogenes.

According to a first aspect of the invention, peptide of the present invention is by the operon coding, and it comprises any nucleotide sequence from streptococcus pyogenes as follows: SEQ ID NO:1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,39,41,43,45,47,49,51,53,55,57,59 and 61, or its homologue in gram positive bacterium, or its function fragment, be used for the treatment of or diagnose.

Described peptide infects treatment A group streptococcus can have many therepic use, is included in the purposes in the prophylactic applications vaccine.

According to second aspect, the polynucleotide of peptide as defined above of encoding also can be used for the treatment of or diagnose.

According to the third aspect, the gene of the described peptide of encoding can be used for preparing attenuated microorganisms.Attenuated microorganisms has a sudden change usually, and this sudden change destroys the expression of one or more this paper genes identified, and the bacterial strain that lacks virulence is provided.These microorganisms also can be used for the treatment of and diagnose.

According to fourth aspect, according to the illness that peptide of the present invention, gene and attenuated microorganisms can be used for treating or prevention is relevant with suis or gram positive bacteria infection.

Detailed Description Of The Invention

The discovery of the gene of the peptide that the present invention is based on encodes relates to virulence.Therefore peptide of the present invention and gene can be used for preparing the therapeutical agent that treatment is infected.The treatment that should be appreciated that indication also comprises prophylactic treatment, for example, and vaccination.In addition, although product of the present invention mainly is intended to treat the infection of human patients, the animal doctor uses and also is considered within the scope of the invention.

The present invention is described with reference to streptococcus pyogenes, yet all A group streptococcus bacterial strains and many other Gram-positive bacteria strains also may comprise related peptides or the protein that the protein identified with this paper has amino acid sequence identity or similarity.The biology that may comprise described peptide includes but not limited to lactococcus (Lactococcus), enterococcus spp (Enterococcus), streptococcus (Streptococcus) and Staphylococcus (Staphylococcus).

The peptide that the peptide that can be used for all respects of the present invention is preferably identified with this paper has the similarity greater than 40%.More preferably described peptide has the sequence similarity greater than 60%.Most preferably described peptide has the sequence similarity greater than 80%, for example 95% similarity.About this paper identified polynucleotides sequence, the related polynucleotides sequence that can be used for all respects of the present invention can have the identity greater than 40% with the sequence that this paper identifies.More preferably described polynucleotide sequence has the sequence identity greater than 60%.Most preferably described polynucleotide sequence has the sequence identity greater than 80%, for example 95% identity.

Term " similarity " and " identity " are known in the art.Used term " identity " is meant based on identical paired sequence between the corresponding same position of institute's comparative sequences relatively.Term " similarity " is meant the comparison between the aminoacid sequence, and not only considers also to consider intimate amino acid on correspondence position at the identical amino acid of correspondence position.Therefore the similarity between the peptide sequence represents also have functional similarity except that sequence similarity.

Identity between between identity level between the gene order and aminoacid sequence or similarity level can use known method to calculate.For the present invention, the computer based that can openly obtain determines that the method for identity and similarity comprises BLASTP, BLASTN and FASTA (Atschul etc., molecular biology magazine (J.Molec.Biol.), 1990; 215:403-410), available from the BLASTX program of NCBI with available from Genetics Computer Group, the Gap program of Madison WI.Similarity provided herein and identity level are to use the Gap program to obtain, with Gap penalize be 12 and Gap length to penalize be 4 to determine aminoacid sequence relatively, with Gap penalize be 50 and Gap length to penalize be 3 to carry out the polynucleotide sequence comparison.

Characterized according to gene of the present invention, thereby described gene order may be used at other microorganism search genes involved or peptide.This can be undertaken by searching in existing database, for example, and EMBL or GenBank.

Can carry out purifying and separate with method well known in the art according to peptide of the present invention or protein.Specifically, identify described gene order, thereby may use recombinant technology expressing said gene in appropriate host.But identified activity fragment and associated molecule are also used it for treatment.For example, peptide or its active fragments can be used for vaccine as antigenic determinant, excite immunne response.Also they can be used for Antibody Preparation, carry out passive immunization or be used for diagnosis.Suitable antibody comprises monoclonal antibody, or its fragment, comprises strand Fv fragment.The method for preparing antibody it will be apparent to those skilled in the art that.

The active fragments of described peptide is those fragments that keep the biological function of described peptide.For example, when being used to excite immunne response, this fragment has enough sizes, and the feasible antibody capable that is generated by this fragment is distinguished other peptides on described peptide and the bacterial micro-organism.Usually this segmental size is at least 30 Nucleotide (10 amino acid), and preferred 60 Nucleotide (20 amino acid) are most preferably greater than 90 Nucleotide (30 amino acid).

Be appreciated that also that except associated molecule the present invention includes the modification that the peptide and the polynucleotide of this paper evaluation are done, described modification does not significantly change its biological function from other microorganisms.The genetic code degeneracy can produce to have to compare with the indicated sequence of this paper has only little sequence change but still the polynucleotide of the identical peptide of encoding, and this it will be apparent to those skilled in the art that.The complementary polynucleotide are also included within the present invention.Conservative substitution on amino acid levels also can be infered, and promptly different acidity or basic aminoacids can be replaced, and function does not have substantial loss.

Preparation is known based on the vaccine of attenuated microorganisms to those skilled in the art.Available suitable carriers or adjuvant, for example alum as composition necessity or desirable, is prepared vaccine composition, to provide effective immunity to anti-infective.The preparation of vaccine preparation it will be apparent to those skilled in the art that.Can prepare attenuated microorganisms, it has the sudden change of any expression of gene of destroying this paper evaluation.The method that the known destruction specific gene of those skilled in the art is expressed.Adoptable technology comprises inactivation or the genetically deficient technology inserted.Also can comprise the other sudden change of other genes according to attenuated microorganisms of the present invention, for example, second gene identifying at this paper or on the needed independent gene of microorganism growth suddenlys change as aro.Attenuated microorganisms can also be used as the carrier system of sending heterologous antigen, therapeutic protein or nucleic acid (DNA or RNA).In this embodiment, described attenuated microorganisms is used to send heterologous antigen, protein or nucleic acid specific site to the body.Can heterologous antigen, peptide or nucleic acid be introduced attenuated microorganisms by traditional technology, these technology comprise utilizes recombinant precursor, and as carrier, it comprises the polynucleotide of expressing heterologous antigen or therapeutic protein, also comprises suitable promoter sequence.Perhaps, coding heterologous antigen or proteinic gene can be mixed in the genome of organism, and use endogenesis promoter control to express.

More generally, also be well known to those skilled in the artly to be, can select the activeconstituents of the present invention of appropriate amount, and suitable carriers or vehicle and route of administration, be used for the treatment of.Can select or definite these factors according to known index, as sanatory character/severity, curee's type and/or healthy state etc.

At one independently in the embodiment, product of the present invention can be used for identifying effective antimicrobial agents, or detects the shaker test of virulence.Conventional shaker test is well known by persons skilled in the art, also can make an amendment, and uses product of the present invention with appropriate means.For example, can be with the target of product of the present invention as active drug, described medicine energy deactivation or combining target show that then medicine has the potential antimicrobial acivity.

Various product of the present invention also can be used for the animal doctor and use.

Be the brief overview that is used to identify the test method of virulence gene below.In the streptococcus pyogenes mutant library, screen the attenuation mutant by use characteristic mark mutagenesis (STM) and identify the virulence gene of streptococcus pyogenes (Hensel etc., 1995. science (Science) 269 (5222): 400-3).

Use plasmid vector, insert by the Tn917 transposon and produce mutant.Except that the Tn917 fragment, used carrier also comprises streptomycin resistance gene, has the paraxin drug resistant gene (CAT gene) of synthetic promoter, the cloning site of Gram-negative bacteria replication orgin (rop.ori) and STM mark.After mark connected into carrier, carry out the intestinal bacteria conversion, select 96 plasmids with initial flagging hybridization.

Transform streptococcus pyogenes bacterial strain B514-SM (M50 type) respectively with each of 96 marker plasmids of gained, go out transformant by resistance screening to Streptomycin sulphate and paraxin.To each streptococcus pyogenes bacterial strain, insert 20 mutant of generation by the Tn917 transposon and create a mutant library through transforming.

In the skin aggressive damage model of mouse infection, screen mutant library (Schrager etc., Journal of Clinical Investigation (J.Clinical Investigation) 1996; 98:1954-1958), or from the throat field planting model discrimination mutant library of mouse infection (Husmann etc. infect and immune (Infection and Immunity) 1997; 65 (4): 935-944).

In skin model, be used in 1 * 10 in the 50 μ l volumes ⁸Cell carries out intradermal vaccination separately to five CD1 mouse, and these cells are represented the set of 96 mutant different and that be easy to distinguish.In inoculation back 48 hours, bacterium was also reclaimed in sampling.Skin decreased being immersed in 2 * BHI substratum, bacterium discharged from skin decreases by in bacterium separator, handling 10 minutes.With the bacterium bed board that discharges, reclaim minimum 10,000 bacterium colonies from minimum 3 mouse.DNA isolation and the being used for DNA of the mark in reclaiming bacterium that increases from these samples.To reclaim the storehouse separated DNA as hybridization probe from each, be presented at can not reclaim from animal in each storehouse, therefore those mutant of attenuation this animal infection modal.

In throat field planting model, with 2 * 10 ⁸Individual bacterium intranasal vaccination to 6 a C57BL/6 mouse, sampling after 48 hours.The same with skin model, the bacterium that contains transposon Tn917 insertion in virulence gene can not be reclaimed from the mouse that mixes group's inoculation with mutant, may be attenuation therefore.

Mutant by the STM Screening and Identification is carried out additional test, determined competitive index (CI).Each mutant of test in the polyinfection of carrying out with the wild type strain in the skin damage model that infects.(Chiang, S.L. and Mekalanos, J.J. molecular microbiology (MolecularMicrobiology) 1998; 27 (4) 797-805). for initial screening, with the wild-type and the mutant cell of equal number, to ading up to 1 * 10 ⁸Cell inoculation contains the group of 4 CD1 mouse.The bacterium of reclaiming after 48 hours is taped against on the selective medium flat board, thereby can distinguishes wild-type bacterium colony and mutant bacterium colony.The ratio of mutant bacteria and wild-type bacterium and the ratio that reclaims this ratio in the bacterium are competitive index (in the inoculum mutant to the ratio of wild-type divided by the ratio of the mutant that reclaims from animal model to wild-type bacterium) in inoculum

The following example describes the present invention in detail.

Embodiment 1

Identify first mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, nucleotide level show with the streptococcus pyogenes genome in the identity of encoding sequence 100% shown in SEQID NO.1, this encoding sequence infer protein sequence shown in SEQ ID NO.2.

The aminoacid sequence of protein of prediction shows that (preserving number: the NAD that SW:Q57431) infers (P) H nitroreductase has 43% identity with hemophilus influenzae (H.influenzae).

Similarity in view of this gene of the NAD that infers (P) the H nitroreductase gene of streptococcus pyogenes and hemophilus influenzae it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism also may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.644.

Embodiment 2

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, nucleotide level show with the streptococcus pyogenes genome in sequence shown in SEQID NO.3 98% identity is arranged.Its aminoacid sequence of inferring is shown in SEQ ID NO.4.

Described aminoacid sequence shows that (preserving number: TREMBL:069155) possible intergrase has 81% identity with Streptococcus mutans (S.mutans).

This proof, the possible integrase gene of ruined gene and Streptococcus mutans is identical to small part.Yet this gene before had been unknown in streptococcus pyogenes, did not think that it works in virulence.

Similarity in view of the possible integrase gene of described streptococcus pyogenes gene and Streptococcus mutans it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism also is a virulence determinant.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.229.

Embodiment 3

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, nucleotide level show with the streptococcus pyogenes genome in sequence shown in SEQID NO.5 97% identity is arranged.The aminoacid sequence of its prediction is shown in SEQ ID NO.6.

Described aminoacid sequence shows and unicellular cyanobacteria Synechocystis at amino acid levels.(preserving number: GlgP albumen TREMBL:P73511) has 37% identity to spp.

This proof, ruined gene is identical to small part with the glgP gene of Synechocystis.spp.

Similarity in view of the glgP gene of described streptococcus pyogenes gene and Synechocystis.spp it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism also is a virulence determinant.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.011.

Embodiment 4

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, nucleotide level show with the streptococcus pyogenes genome in sequence shown in SEQID NO.7 97% identity is arranged.The aminoacid sequence of its prediction is shown in SEQ ID NO.8.

Described aminoacid sequence shows at amino acid levels that (preserving number: BraB albumen TREMBL:034545) has 35% identity with subtilis (B.subtilis).。

This proof, ruined gene is identical to small part with the braB gene of subtilis.

Similarity in view of the braB gene of the braB gene of described streptococcus pyogenes and subtilis it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism also may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.88.

Embodiment 5

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, nucleotide level show with the streptococcus pyogenes genome in sequence shown in SEQID NO.9 99% identity is arranged.The aminoacid sequence of its prediction is shown in SEQ ID NO.10.

Also identified another attenuation mutant, its nucleotides sequence is listed in that the sequence shown in SEQ ID NO.11 has 98% identity in nucleotide level and the streptococcus pyogenes genome.The aminoacid sequence of its prediction is shown in SEQ ID NO.12.

(preserving number: AdcR albumen TREMBL:033703) has 53% identity to the predicted protein matter of first mutant amino acid levels and streptococcus pneumoniae (S.pneumoniae).(preserving number: AdcC albumen TREMBL:087862) has 79% identity to the predicted protein matter of second mutant amino acid levels and streptococcus pneumoniae.

This proof, ruined gene is identical to small part with adcR gene and the adcC gene of streptococcus pneumoniae.AdcR gene and adcC gene are the parts of (comprising adcR, adcC, adcB and adcA) of adc operon in the streptococcus pneumoniae.Therefore the virulence attenuation of of streptococcus pyogenes adcR and adcC mutant may be by due to the downstream gene that can not express adcR and adcC gene or its prediction (adcB and adcA homologous gene).Before do not think that these genes worked in the virulence of streptococcus pyogenes.

Similarity in view of adcR gene and the adcC gene of described streptococcus pyogenes gene and streptococcus pneumoniae it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.548 (adcR) and 0.028 (adcC).

Embodiment 6

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, nucleotide level show with the streptococcus pyogenes genome in sequence shown in SEQID NO.13 99% identity is arranged.The aminoacid sequence of its prediction is shown in SEQ ID NO.14.

Described aminoacid sequence shows at amino acid levels that (preserving number: dna repair protein radC homologue (orfB) SW:Q02170) has 39% identity with subtilis.

This proof, ruined gene is identical to small part with the orfB gene of subtilis.

Similarity in view of the orfB gene of the orfB gene of described streptococcus pyogenes and subtilis it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.766.

Embodiment 7

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, nucleotide level show with the streptococcus pyogenes genome in sequence shown in SEQID NO.15 98% identity is arranged.The aminoacid sequence of its prediction is shown in SEQ ID NO.16.

Described aminoacid sequence shows at amino acid levels that (preserving number: biotin carboxyl carrier protein SW:P29337) (BCCP) has 54% identity with Streptococcus mutans.

This proof, ruined gene is identical to small part with the gene of coding Streptococcus mutans BCCP.This gene before had been unknown in streptococcus pyogenes, did not think that it works in virulence.

Similarity in view of the streptococcus pyogenes gene and the Streptococcus mutans BCCP of described translation it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.044.

Embodiment 8

Identify that a series of and the gene that relates to citric acid fermentation have the mutant of sequence similarity.The nucleotide sequence of eight mutant show with the streptococcus pyogenes genome in the identity of sequence 100% shown in SEQ ID NO.17.The aminoacid sequence of its prediction is shown in SEQ ID NO.18.

(preserving number: citF albumen SW:P75726), citrate lyase α chain has 58% identity to the predicted protein of described streptococcus pyogenes gene amino acid levels and intestinal bacteria (E.coli).

In addition the nucleotide sequence of three mutant show with the streptococcus pyogenes genome in different sequences 99% identity is arranged.Described nucleotide sequence is shown in SEQ ID NO.19, and the aminoacid sequence of its prediction is shown in SEQ ID NO.20.

(preserving number: citE albumen SW:P77770), citrate lyase β chain has 55% identity to described aminoacid sequence amino acid levels and intestinal bacteria.

In addition the nucleotide sequence of two mutant show with streptococcus pyogenes in nucleotides sequence shown in SEQ ID NO.21 show 99% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.22.

(preserving number: CitD albumen SW:P77618), citrate lyase acyl carrier protein (citrate lyase γ chain) has 46% identity to described aminoacid sequence amino acid levels and intestinal bacteria.

Identified in addition two mutant, its nucleotide sequence show with streptococcus pyogenes in nucleotides sequence shown in SEQ IDNO.23 show 99% identity.The aminoacid sequence of its prediction is shown in SEQ IDNO.24.

(preserving number: CitC albumen SW:P77390), citric acid (pro-3s)-lyase (ligase enzyme) has 34% identity to described aminoacid sequence amino acid levels and intestinal bacteria.

Identified another mutant, its nucleotide sequence show with streptococcus pyogenes in nucleotides sequence shown in SEQ IDNO.25 show 99% sequence identity.The aminoacid sequence of its prediction is shown in SEQID NO.26.

(preserving number: CitX albumen SW:P77563) has 37% identity to described aminoacid sequence amino acid levels and intestinal bacteria.

Identified in addition three mutant, its nucleotide sequence show with streptococcus pyogenes in nucleotides sequence shown in SEQ IDNO.27 show 100% identity.The aminoacid sequence of its prediction is shown in SEQ IDNO.28.

Described aminoacid sequence shows at amino acid levels that (preserving number: OadA albumen SW:P13187), oxaloacetic decarboxylase α subunit has 59% identity with Klebsiella pneumonia (K.pneumoniae).

The predicted protein matter sequence table of all said mutation bodies reveal with intestinal bacteria or Klebsiella pneumonia in the required gene product of citric acid fermentation have in various degree identity.

This proof, the citF of ruined gene and intestinal bacteria or Klebsiella pneumonia, E, D, C, X is identical to small part with the oadA gene.The single site of gene co on intestinal bacteria and Klebsiella pneumonia karyomit(e) that relates to citric acid fermentation.Therefore, the virulence attenuation of of streptococcus pyogenes mutant may be by not expressing due to its citFEDCX and oadA gene or the downstream gene.Do not thought before that these genes worked in virulence.

In view of the similarity of those genes that exist in described streptococcus pyogenes gene and intestinal bacteria or the Klebsiella pneumonia, it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence.

To each citF, E, D, the mutant of X and oadA is tested weakening of virulence.Mutant microbial shows virulence attenuation of, and its competitive index (CI) is 0.122 (citF), 0.064 (citE), 0.078 (citD), 0.025 (citX) and 0.251 (oadA).

Embodiment 9

Identified another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotides sequence be listed in nucleotide level show with the streptococcus pyogenes genome in sequence shown in SEQID NO.29 98% identity is arranged.The aminoacid sequence of its prediction is shown in SEQ ID NO.30.

Described aminoacid sequence shows at amino acid levels that (preserving number: femB albumen TREMBL:Q9X9D7) has 27% identity with streptococcus aureus (S.aureus).

This proof, ruined gene is identical to small part with the femB gene of streptococcus aureus.Yet this gene before had been unknown in streptococcus pyogenes, did not think that it works in virulence.

Similarity in view of the femB gene of described streptococcus pyogenes gene and streptococcus aureus it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence.

Embodiment 10

Identified another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.31 has significant identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.32.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.160.

Embodiment 11

Identified another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.33 has 100% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.34.

Embodiment 12

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, the sequence in nucleotide level and streptococcus pyogenes genome shown in SEQ IDNO.35 has 98% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.36.

Described aminoacid sequence shows at amino acid levels that (preserving number: SW:P33116) subtilyne transhipment ATP is conjugated protein 46% identity with subtilis.

This proof, ruined gene is identical to small part with the braB gene of subtilis.

Similarity in view of the gene of the gene of described streptococcus pyogenes and subtilis it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.128.

Embodiment 13

Identified another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.37 has 98% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.38.

Embodiment 14

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.39 has 98% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.40.

Described aminoacid sequence shows at amino acid levels that (preserving number: 36.9kDa hypothetical protein SW:P33019) has 29% identity with intestinal bacteria.

This proof, ruined gene is identical to small part with colibacillary described gene.Other similar sequences may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.747.

Embodiment 15

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.41 has 99% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.42.

Described aminoacid sequence shows and glimmers that (preserving number: 89kDa hypothetical protein TREMBL:028455) has 29% identity to ancient green-ball bacterium (A.fulgidus) at amino acid levels.

This proof, ruined gene is identical to small part with colibacillary described gene.Other similar sequences may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.588.

Embodiment 16

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.43 has 99% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.44.

Embodiment 17

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.45 has 98% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.46.

Embodiment 18

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.47 has 97% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.48

Embodiment 19

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, the sequence in nucleotide level and streptococcus pyogenes genome shown in SEQ IDNO.49 has 98% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.50.

Described aminoacid sequence shows at amino acid levels that (preserving number: ciaH albumen SW:Q54955) has 47% identity with streptococcus pneumoniae.

This proof, ruined gene is identical to small part with the ciaH gene of streptococcus pneumoniae.

Similarity in view of the ciaH gene of the ciaH gene of described streptococcus pyogenes and streptococcus pneumoniae it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.205.

Embodiment 20

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, the sequence in nucleotide level and streptococcus pyogenes genome shown in SEQ IDNO.51 has 98% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.52.

Described aminoacid sequence shows at amino acid levels that (preserving number: mucA homologous protein TREMBL:Q9ZBB1) has 39% identity with streptococcus pneumoniae.

This proof, ruined gene is identical to small part with the mucA gene of streptococcus pneumoniae.

Similarity in view of the mucA gene of the gene of described streptococcus pyogenes and streptococcus pneumoniae it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.547.

Embodiment 21

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, the sequence in nucleotide level and streptococcus pyogenes genome shown in SEQ IDNO.53 has 98% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.54.

Described aminoacid sequence shows at amino acid levels that (preserving number: gidA albumen SW:P39815) has 69% identity with subtilis.

This proof, ruined gene is identical to small part with the described gene of subtilis.

Similarity in view of the gidA gene of the gene of described streptococcus pyogenes and subtilis it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.444.

Embodiment 22

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, the sequence in nucleotide level and streptococcus pyogenes genome shown in SEQ IDNO.55 has 98% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.56.

Described aminoacid sequence shows at amino acid levels that (preserving number: dltA albumen SW:Q53526) has 74% identity with Streptococcus mutans.

This proof, ruined gene is identical to small part with the dltA gene of Streptococcus mutans.

Similarity in view of the dltA gene of the gene of described streptococcus pyogenes and Streptococcus mutans it will be appreciated by those skilled in the art that the similar sequence in other gram-positive microorganisms may relate to virulence.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.205.

Embodiment 23

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Described nucleotide sequence, the sequence in nucleotide level and streptococcus pyogenes genome shown in SEQ IDNO.57 has 98% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.58.

Described aminoacid sequence shows and glimmers that (preserving number: hmgA albumen SW:O28538) has 41% identity to ancient green-ball bacterium at amino acid levels.

This proof, ruined gene is identical to small part with the described gene of the ancient green-ball bacterium of flicker.

In view of the gene of described streptococcus pyogenes similarity, it will be appreciated by those skilled in the art that the similar sequence in other suis and gram-positive microorganism may relate to virulence with the hmgA gene of the ancient green-ball bacterium of flicker.

In the virulence attenuation of test, mutant microbial demonstrates virulence attenuation of, and its competitive index (CI) is 0.205.

Embodiment 24

Identify first mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.59 has 99% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.60.

Described aminoacid sequence shows at amino acid levels that (preserving number: the JAG albumen of inferring TREMBL:Q9X1H1) has 34% identity with Thermotoga maritima (T.maritima).

This proof, the gene of ruined streptococcus pyogenes is identical to small part with the described gene of Thermotoga maritima (T.maritima).Similar sequence in other Gram-negative bacterias may relate to virulence.

Embodiment 25

Identify another mutant, and the clone is right after the nucleotide sequence that transposon inserts.

Sequence in described nucleotide sequence and the streptococcus pyogenes genome shown in SEQ ID NO.61 has 99% identity.The aminoacid sequence of its prediction is shown in SEQ ID NO.62.

Described aminoacid sequence shows at amino acid levels that (preserving number: 49.4kDa hypothetical protein TREMBL:Q9X4U3) has 49% identity with swine streptococcus (S.suis).

This proof, the gene of ruined streptococcus pyogenes is identical to small part with the described gene of swine streptococcus.Similar sequence in other Gram-negative bacterias also may relate to virulence.

Sequence table

<110>Microscience?Limited

＜120〉virulence gene, protein and uses thereof

<130>REP06309WO

＜140〉(the unknown)

<141>2000-12-22

<150>9930462.8

<151>1999-12-23

<150>9930463.6

<151>1999-12-23

<150>9930464.4

<151>1999-12-23

<150>9930466.9

<151>1999-12-23

<150>9930467.7

<151>1999-12-23

<150>9930469.3

<151>1999-12-23

<150>9930471.9

<151>1999-12-23

<150>9930472.7

<151>1999-12-23

<150>9930473.5

<151>1999-12-23

<150>9930474.3

<151>1999-12-23

<150>9930475.0

<151>1999-12-23

<150>9930476.8

<151>1999-12-23

<150>0003735.8

<151>2000-02-17

<150>0003733.3

<151>2000-02-17

<150>0003732.5

<150>0003733.3

<151>2000-02-17

<150>0003732.5

<151>2000-02-17

<150>0003731.7

<151>2000-02-17

<150>0003736.6

<151>2000-02-17

<150>0003730.9

<151>2000-02-17

<150>0003729.1

<151>2000-02-17

<150>0003728.3

<151>2000-02-17

<150>0003727.5

<151>2000-02-17

<150>0003726.7

<151>2000-02-17

<150>0003725.9

<151>2000-02-17

<150>0010587.4

<151>2000-05-02

<150>0010585.8

<151>2000-05-02

<160>62

<170>PatentIn?Ver.2.1

<210>1

<211>666

<212>DNA

＜213〉streptococcus pyogenes

<400>1

atggatcaaa?ccattcatca?ccaaatacag?caagcactac?actttagaac?agccgttcgt?60

gtttataagg?aagaaaagat?ttctgatgag?gatttagccc?ttatccttga?tgctgcttgg?120

ttaagccctt?cttctattgg?cttagaaggc?tggcgctttg?tcgttttaga?caacaagcct?180

attaaagaag?aaatcaagcc?ctttgcctgg?ggagcccagt?atcaactgga?aacagctagt?240

cactttattc?ttttaatagc?cgaaaaacat?gcaagatacg?atagccctgc?tatcaaaaat?300

agccttttac?ggcgtggtat?caaagaaggt?gatggtctca?acagccgcct?aaaactctat?360

gaatctttcc?aaaaagagga?catggatatg?gcagataatc?ctcgggcact?ctttgattgg?420

acagctaaac?aaacctatat?cgctcttggt?aatatgatga?tgacagctgc?tcttttgggc?480

attgatactt?gccctattga?aggctttcat?tatgataagg?tcaatcatat?cctagctaag?540

cataatgtga?ttgatttaga?aaaagaaggc?attgctagca?tgttatccct?aggctatcgt?600

ttgcgagatc?ccaaacatgc?tcaagttcgt?aagcctaaag?aagaagtgat?ttcagtcgtt?660

aaatga 666

<210>2

<211>221

<212>PRT

＜213〉streptococcus pyogenes

<400>2

Met?Asp?Gln?Thr?Ile?His?His?Gln?Ile?Gln?Gln?Ala?Leu?His?Phe?Arg

1 5 10 15

Thr?Ala?Val?Arg?Val?Tyr?Lys?Glu?Glu?Lys?Ile?Ser?Asp?Glu?Asp?Leu

20 25 30

Ala?Leu?Ile?Leu?Asp?Ala?Ala?Trp?Leu?Ser?Pro?Ser?Ser?Ile?Gly?Leu

35 40 45

Glu?Gly?Trp?Arg?Phe?Val?Val?Leu?Asp?Asn?Lys?Pro?Ile?Lys?Glu?Glu

50 55 60

Ile?Lys?Pro?Phe?Ala?Trp?Gly?Ala?Gln?Tyr?Gln?Leu?Glu?Thr?Ala?Ser

65 70 75 80

His?Phe?Ile?Leu?Leu?Ile?Ala?Glu?Lys?His?Ala?Arg?Tyr?Asp?Ser?Pro

85 90 95

Ala?Ile?Lys?Asn?Ser?Leu?Leu?Arg?Arg?Gly?Ile?Lys?Glu?Gly?Asp?Gly

100 105 110

Leu?Asn?Ser?Arg?Leu?Lys?Leu?Tyr?Glu?Ser?Phe?Gln?Lys?Glu?Asp?Met

115 120 125

Asp?Met?Ala?Asp?Asn?Pro?Arg?Ala?Leu?Phe?Asp?Trp?Thr?Ala?Lys?Gln

130 135 140

Thr?Tyr?Ile?Ala?Leu?Gly?Asn?Met?Met?Met?Thr?Ala?Ala?Leu?Leu?Gly

145 150 155 160

Ile?Asp?Thr?Cys?Pro?Ile?Glu?Gly?Phe?His?Tyr?Asp?Lys?Val?Asn?His

165 170 175

Ile?Leu?Ala?Lys?His?Asn?Val?Ile?Asp?Leu?Glu?Lys?Glu?Gly?Ile?Ala

180 185 190

Ser?Met?Leu?Ser?Leu?Gly?Tyr?Arg?Leu?Arg?Asp?Pro?Lys?His?Ala?Gln

195 200 205

Val?Arg?Lys?Pro?Lys?Glu?Glu?Val?Ile?Ser?Val?Val?Lys

210 215 220

<210>3

<211>1071

<212>DNA

＜213〉streptococcus pyogenes

<400>3

atgagacgcg?aattattact?agaaaaaatt?gaaacctaca?aggctatcat?gccctggtat?60

gttttagatt?attaccaatc?caaattagct?gttccataca?gctttaccac?cttatatgag?120

tatttaaaag?aatacaaacg?cttttttgat?tggctgatgg?atgctgattt?aacgcaggcg?180

ccaaagattg?ctgatattga?cttgagcacg?cttgagcacc?ttaccaagaa?ggatttagag?240

gcctttgtgc?tttatttgcg?ggaacgacct?tctctcaaca?cctattccac?caaagagggg?300

ctttctcaaa?ccactattaa?tcggaccttg?tctgccttat?caagccttta?caagtacttg?360

actgaagagg?ttgaaaatga?ccaaggggag?ccttattttt?atcggaatgt?tatgaaaaaa?420

gtgtcaacaa?aaaagaaaaa?agaaaccctg?gcttccagag?cggaaaatat?taagcagaaa?480

ctgtttttag?gtgatgaaac?tctagctttc?ttggattatg?tggataagga?atatgagcaa?540

aaattgtcta?atcgtgccaa?atcttctttc?cgtaaaaata?aggagaggga?tttggctatt?600

attgctctac?tacttgcttc?tggtgttcgt?ttgtctgaag?cagtgaatct?tgatttaaaa?660

gatgtcaatc?taaatatgat?gattatcgaa?gttattcgca?aaggcggaaa?acgtgattca?720

gtcaatgtag?caggttttgc?gaaaggttat?ttggagtctt?atttagctgt?tcgtcagaga?780

cgttacaagg?ctgaaaaaca?ggatcttgct?ttctttttaa?cagaataccg?tggcgttcca?840

aatcgaatgg?atgcttctag?tatagaaaag?atggttggca?agtactctga?ggattttaaa?900

attcgtgtga?ctcctcacaa?attgcgtcat?acgctagcga?caagactata?tgatgctact?960

aaatctcagg?tgttagttag?tcatcaatta?ggccattcct?ctactcaagt?cacggatctt?1020

tatacccata?ttgtcaatga?tgagcaaaaa?aatgctttag?ataaccttta?a 1071

<210>4

<211>356

<212>PRT

＜213〉streptococcus pyogenes

<400>4

Met?Arg?Arg?Glu?Leu?Leu?Leu?Glu?Lys?Ile?Asp?Glu?Leu?Lys?Glu?Leu

1 5 10 15

Met?Pro?Trp?Tyr?Val?Leu?Glu?Tyr?Tyr?Gln?Ser?Lys?Leu?Thr?Val?Pro

20 25 30

Tyr?Ser?Phe?Thr?Thr?Leu?Tyr?Glu?Tyr?Leu?Lys?Glu?Tyr?Arg?Arg?Phe

35 40 45

Phe?Glu?Trp?Leu?Ile?Asp?Ser?Gly?Val?Ser?Asn?Ala?Asn?Lys?Leu?Ala

50 55 60

Asp?Ile?Pro?Leu?Glu?Thr?Leu?Glu?His?Leu?Ser?Lys?Lys?Asp?Met?Glu

65 70 75 80

Ser?Phe?Ile?Leu?Tyr?Leu?Arg?Glu?Arg?Thr?Leu?Leu?Asn?Thr?Lys?Asn

85 90 95

Lys?Arg?Gln?Gly?Val?Ser?Gln?Thr?Thr?Ile?Asn?Arg?Thr?Leu?Ser?Ala

100 105 110

Leu?Ser?Ser?Leu?Tyr?Lys?Tyr?Leu?Thr?Glu?Glu?Val?Glu?Asn?Ala?Asp

115 120 125

Gly?Glu?Pro?Tyr?Phe?Tyr?Arg?Asn?Val?Met?Lys?Lys?Val?Ser?Thr?Lys

130 135 140

Lys?Lys?Lys?Glu?Thr?Leu?Ala?Ala?Arg?Ala?Glu?Asn?Ile?Lys?Gln?Lys

145 150 155 160

Leu?Phe?Leu?Gly?Asn?Glu?Thr?Met?Glu?Phe?Leu?Glu?Tyr?Val?Asp?Cys

165 170 175

Glu?Tyr?Glu?Gln?Lys?Leu?Ser?Lys?Arg?Ala?Leu?Ser?Ser?Phe?Arg?Lys

180 185 190

Asn?Lys?Glu?Arg?Asp?Leu?Ala?Ile?Ile?Ala?Leu?Leu?Leu?Ala?Ser?Gly

195 200 205

Val?Arg?Leu?Ser?Glu?Ala?Val?Asn?Leu?Asp?Leu?Lys?Asp?Val?Asn?Leu

210 215 220

Asn?Met?Met?Ile?Ile?Glu?Val?Thr?Arg?Lys?Gly?Gly?Lys?His?Asp?Ser

225 230 235 240

Val?Asn?Val?Ala?Gly?Phe?Ala?Lys?Pro?Tyr?Leu?Glu?Asn?Tyr?Ile?Thr

245 250 255

Ile?Arg?Arg?Gly?Arg?Tyr?Lys?Ala?Lys?Lys?Thr?Asp?Leu?Ala?Phe?Phe

260 265 270

Leu?Ser?Glu?Tyr?Arg?Gly?Val?Pro?Asn?Arg?Met?Asp?Ala?Ser?Ser?Ile

275 280 285

Glu?Lys?Met?Val?Ala?Lys?Tyr?Ser?Gln?Asp?Phe?Lys?Ile?Arg?Val?Thr

290 295 300

Pro?His?Lys?Leu?Arg?His?Thr?Leu?Ala?Thr?Arg?Leu?Tyr?Asp?Ala?Thr

305 310 315 320

Lys?Ser?Gln?Val?Leu?Val?Ser?His?Gln?Leu?Gly?His?Ala?Ser?Thr?Gln

325 330 335

Val?Thr?Asp?Leu?Tyr?Thr?His?Ile?Val?Asn?Asp?Glu?6ln?Lys?Asn?Ala

340 345 350

Leu?Asp?Lys?Leu

355

<210>5

<211>2265

<212>DNA

＜213〉streptococcus pyogenes

<400>5

atgacacgct?ttacagaata?tgttgaaact?aaactaggaa?aatcacttac?gcaagccagc?60

aatgaagaga?tttatctatc?attattaaac?tttgtcaaag?aagaagctag?ccacaaggct?120

aaaaattctg?ctaaacgcaa?agtttactat?atctcagcag?agtttttgat?tggtaaatta?180

ctatcaaaca?acctgattaa?cctaggaatt?tacaaggaca?tcaaagaaga?attggcagct?240

gctggcaagt?ctatcgcaga?agtcgaagat?gttgaattag?aaccatcact?aggtaatggt?300

ggtttaggac?gccttgcttc?atgttttatt?gactctattg?catctcttgg?gattaatggt?360

gaaggggttg?gtttaaacta?tcattgtggg?ttgtttaagc?aagtcttcaa?acacaatgag?420

caagaagctg?agccaaactt?ctggattgaa?gatgactcat?ggttggttcc?aacagacatt?480

tcttacgatg?tgccttttaa?gaacttcacc?ttgaaatctc?gtcttgatcg?tattgatgtt?540

ttgggttaca?aacgcgacac?taaaaactac?cttaacttgt?ttgatatcga?gggcgttgat?600

tacgggttaa?tcaaagacgg?catttctttt?gataaaacgc?aaattgctaa?aaacttgacc?660

ttgttcctct?acccagacga?ttctgacaaa?aatggggaat?tgctccgtat?ttaccaacag?720

tactttatgg?tgtcaaatgc?agcgcaatta?atcattgatg?aagctatcga?acgtggttca?780

aaccttcatg?accttgcaga?ctacgcttac?gtgcaaatta?atgacacgca?tccatcaatg?840

gtcatcccag?aattaattcg?tctcttgact?gaaaaacatg?gctttgactt?tgatgaagcg?900

gtagctgttg?tgaaaaatat?ggttggttac?actaaccaca?ctattcttgc?agaagccctt?960

gaaaaatggc?caactgctta?cttaaacgaa?gtagtgccac?acttggtaac?catcattgaa?1020

aaattggatg?ctcttgttcg?ttcagaagtg?tctgatccag?ctgttcaaat?tattgacgaa?1080

tcaggtcgtg?tgcacatggc?ccatatggat?attcattttg?caacaagtgt?caatggggta?1140

gcagcactcc?acacagaaat?cttgaaaaac?agcgaattaa?aagctttcta?tgacctttac?1200

ccagaaaaat?tcaacaacaa?aaccaacggg?attactttcc?gtcgttggct?agaatttgct?1260

aaccaagact?tggctgatta?cattaaagaa?cttattggcg?atgagtactt?gactgacgca?1320

acaaaattag?aaaaattgat?ggcctttgca?gatgacaaag?ctgttcatgc?taaattggct?1380

gaaatcaaat?tcaacaacaa?attagctctt?aaacgttacc?ttaaagacaa?taaagacatt?1440

gagcttgatg?aacattctat?tattgatacc?caaatcaaac?gtttccacga?gtacaaacgt?1500

caacagatga?atgctcttta?cgtgattcac?aaatatttgg?aaattaaaaa?aggcaacctt?1560

ccaaaacgta?aaatcactgt?tatctttgga?ggtaaagcag?cgcctgctta?cattattgcg?1620

caagacatca?ttcacttgat?cctttgcttg?tctgaattga?ttaacaatga?ccctgaagta?1680

agcccatacc?ttaatgtgca?tctagttgaa?aattacaacg?tgacagtagc?agagcacttg?1740

attccagcaa?ctgatatttc?tgagcaaatt?tcactagcat?ccaaagaagc?ttctggaact?1800

ggtaatatga?aattcatgct?taacggtgct?ttaacacttg?gtacaatgga?cggtgctaac?1860

gtagaaatcg?ctgagcttgc?aggcatggag?aatatctata?cctttggtaa?agattctgac?1920

accatcatca?acctttatgc?gactgcttct?tatgtagcaa?aagattacta?tgataaccac?1980

cctgctatta?aagcagcagt?gaactttatt?atcagtccag?aattgctagc?atttggcaac?2040

gaagaacgtc?ttgatcgtct?ttataaagaa?ttgatttcaa?aagactggtt?catgactttg?2100

attgaccttg?aagagtacat?tgaagtgaaa?gaaaaaatgt?tagcagacta?tgaagaccaa?2160

gatttatgga?tgacaaaagt?ggtccataac?atcgcaaaag?ctggattctt?ctcatctgac?2220

cgtaccattg?agcagtataa?cgaagatatt?tggcattcac?gataa 2265

<210>6

<211>754

<212>PRT

＜213〉streptococcus pyogenes

<400>6

Met?Thr?Arg?Phe?Thr?Glu?Tyr?Val?Glu?Thr?Lys?Leu?Gly?Lys?Ser?Leu

1 5 10 15

Thr?Gln?Ala?Ser?Asn?Glu?Glu?Ile?Tyr?Leu?Ser?Leu?Leu?Asn?Phe?Val

20 25 30

Lys?Glu?Glu?Ala?Ser?His?Lys?Ala?Lys?Asn?Ser?Ala?Lys?Arg?Lys?Val

35 40 45

Tyr?Tyr?Ile?Ser?Ala?Glu?Phe?Leu?Ile?Gly?Lys?Leu?Leu?Ser?Asn?Asn

50 55 60

Leu?Ile?Asn?Leu?Gly?Ile?Tyr?Lys?Asp?Ile?Lys?Glu?Glu?Leu?Ala?Ala

65 70 75 80

Ala?Gly?Lys?Ser?Ile?Ala?Glu?Val?Glu?Asp?Val?Glu?Leu?Glu?Pro?Ser

85 90 95

Leu?Gly?Asn?Gly?Gly?Leu?Gly?Arg?Leu?Ala?Ser?Cys?Phe?Ile?Asp?Ser

100 105 110

Ile?Ala?Ser?Leu?Gly?Ile?Asn?Gly?Glu?Gly?Val?Gly?Leu?Asn?Tyr?His

115 120 125

Cys?Gly?Leu?Phe?Lys?Gln?Val?Phe?Lys?His?Asn?Glu?Gln?Glu?Ala?Glu

130 135 140

Pro?Asn?Phe?Trp?Ile?Glu?Asp?Asp?Ser?Trp?Leu?Val?Pro?Thr?Asp?Ile

145 150 155 160

Ser?Tyr?Asp?Val?Pro?Phe?Lys?Asn?Phe?Thr?Leu?Lys?Ser?Arg?Leu?Asp

165 170 175

Arg?Ile?Asp?Val?Leu?Gly?Tyr?Lys?Arg?Asp?Thr?Lys?Asn?Tyr?Leu?Asn

180 185 190

Leu?Phe?Asp?Ile?Glu?Gly?Val?Asp?Tyr?Gly?Leu?Ile?Lys?Asp?Gly?Ile

195 200 205

Ser?Phe?Asp?Lys?Thr?Gln?Ile?Ala?Lys?Asn?Leu?Thr?Leu?Phe?Leu?Tyr

210 215 220

Pro?Asp?Asp?Ser?Asp?Lys?Asn?Gly?Glu?Leu?Leu?Arg?Ile?Tyr?Gln?Gln

225 230 235 240

Tyr?Phe?Met?Val?Ser?Asn?Ala?Ala?Gln?Leu?Ile?Ile?Asp?Glu?Ala?Ile

245 250 255

Glu?Arg?Gly?Ser?Asn?Leu?His?Asp?Leu?Ala?Asp?Tyr?Ala?Tyr?Val?Gln

260 265 270

Ile?Asn?Asp?Thr?His?Pro?Ser?Met?Val?Ile?Pro?Glu?Leu?Ile?Arg?Leu

275 280 285

Leu?Thr?Glu?Lys?His?Gly?Phe?Asp?Phe?Asp?Glu?Ala?Val?Ala?Val?Val

290 295 300

Lys?Asn?Met?Val?Gly?Tyr?Thr?Asn?His?Thr?Ile?Leu?Ala?Glu?Ala?Leu

305 310 315 320

Glu?Lys?Trp?Pro?Thr?Ala?Tyr?Leu?Asn?Glu?Val?Val?Pro?His?Leu?Val

325 330 335

Thr?Ile?Ile?Glu?Lys?Leu?Asp?Ala?Leu?Val?Arg?Ser?Glu?Val?Ser?Asp

340 345 350

Pro?Ala?Val?Gln?Ile?Ile?Asp?Glu?Ser?Gly?Arg?Val?His?Met?Ala?His

355 360 365

Met?Asp?Ile?His?Phe?Ala?Thr?Ser?Val?Asn?Gly?Val?Ala?Ala?Leu?His

370 375 380

Thr?Glu?Ile?Leu?Lys?Asn?Ser?Glu?Leu?Lys?Ala?Phe?Tyr?Asp?Leu?Tyr

385 390 395 400

Pro?Glu?Lys?Phe?Asn?Asn?Lys?Thr?Asn?Gly?Ile?Thr?Phe?Arg?Arg?Trp

405 410 415

Leu?Glu?Phe?Ala?Asn?Gln?Asp?Leu?Ala?Asp?Tyr?Ile?Lys?Glu?Leu?Ile

420 425 430

Gly?Asp?Glu?Tyr?Leu?Thr?Asp?Ala?Thr?Lys?Leu?Glu?Lys?Leu?Met?Ala

435 440 445

Phe?Ala?Asp?Asp?Lys?Ala?Val?His?Ala?Lys?Leu?Ala?Glu?Ile?Lys?Phe

450 455 460

Asn?Asn?Lys?Leu?Ala?Leu?Lys?Arg?Tyr?Leu?Lys?Asp?Asn?Lys?Asp?Ile

465 470 475 480

Glu?Leu?Asp?Glu?His?Ser?Ile?Ile?Asp?Thr?Gln?Ile?Lys?Arg?Phe?His

485 490 495

Glu?Tyr?Lys?Arg?Gln?Gln?Met?Asn?Ala?Leu?Tyr?Val?Ile?His?Lys?Tyr

500 505 510

Leu?Glu?Ile?Lys?Lys?Gly?Asn?Leu?Pro?Lys?Arg?Lys?Ile?Thr?Val?Ile

515 520 525

Phe?Gly?Gly?Lys?Ala?Ala?Pro?Ala?Tyr?Ile?Ile?Ala?Gln?Asp?Ile?Ile

530 535 540

His?Leu?Ile?Leu?Cys?Leu?Ser?Glu?Leu?Ile?Asn?Asn?Asp?Pro?Glu?Val

545 550 555 560

Ser?Pro?Tyr?Leu?Asn?Val?His?Leu?Val?Glu?Asn?Tyr?Asn?Val?Thr?Val

565 570 575

Ala?Glu?His?Leu?Ile?Pro?Ala?Thr?Asp?Ile?Ser?Glu?Gln?Ile?Ser?Leu

580 585 590

Ala?Ser?Lys?Glu?Ala?Ser?Gly?Thr?Gly?Asn?Met?Lys?Phe?Met?Leu?Asn

595 600 605

Gly?Ala?Leu?Thr?Leu?Gly?Thr?Met?Asp?Gly?Ala?Asn?Val?Glu?Ile?Ala

610 615 620

Glu?Leu?Ala?Gly?Met?Glu?Asn?Ile?Tyr?Thr?Phe?Gly?Lys?Asp?Ser?Asp

625 630 635 640

Thr?Ile?Ile?Asn?Leu?Tyr?Ala?Thr?Ala?Ser?Tyr?Val?Ala?Lys?Asp?Tyr

645 650 655

Tyr?Asp?Asn?His?Pro?Ala?Ile?Lys?Ala?Ala?Val?Asn?Phe?Ile?Ile?Ser

660 665 670

Pro?Glu?Leu?Leu?Ala?Phe?Gly?Asn?Glu?Glu?Arg?Leu?Asp?Arg?Leu?Tyr

675 680 685

Lys?Glu?Leu?Ile?Ser?Lys?Asp?Trp?Phe?Met?Thr?Leu?Ile?Asp?Leu?Glu

690 695 700

Glu?Tyr?Ile?Glu?Val?Lys?Glu?Lys?Met?Leu?Ala?Asp?Tyr?Glu?Asp?Gln

705 710 715 720

Asp?Leu?Trp?Met?Thr?Lys?Val?Val?His?Asn?Ile?Ala?Lys?Ala?Gly?Phe

725 730 735

Phe?Ser?Ser?Asp?Arg?Thr?Ile?Glu?Gln?Tyr?Asn?Glu?Asp?Ile?Trp?His

740 745 750

Ser?Arg

<210>7

<211>1371

<212>DNA

＜213〉streptococcus pyogenes

<400>7

atgaaacaaa?aaaatgtata?tatcgtcatt?ggattcatgc?tatttgcgct?attttttgga?60

gcagcaaacc?tcatttaccc?agctttttta?ggcatctact?caggtcatca?gattctatgg?120

tctatcattg?gtttttgttt?aactggtgtc?tccttgcctt?tactcggtgt?cattgctgtt?180

gctaaatctg?gatcaggcga?tgttgaaagt?ttggcacgcc?ccatctctaa?atggtatgca?240

atcttctatt?cttccatttt?gtatttatct?attggcccat?tttttgctat?tccaagaaca?300

ggagccactt?ctttttcagt?cggtatcgct?cctatcttag?gagataatac?aaccaataaa?360

gctatttatg?ctatactatt?ttttggtctg?tcctacttcc?ttgctatcaa?acctagtaaa?420

ctagctgaaa?atatcggaaa?atttttaacg?ccaacgttgt?tagttgttat?ttctattttg?480

gttatcgcgt?cctttgtcca?tcctgctgga?aattacggtg?atgcttttaa?cgctggggtc?540

ggtgttaata?atgcctttaa?agattttcct?tttatagcag?gattaattca?aggttatggc?600

actatggatg?cactagcttc?tcttgttttt?gctattttag?tcattgaggc?taccaaacaa?660

tttggcgcta?agacggacaa?agaaatgacc?aaaataacac?ttatttctgg?ggctattgcc?720

attttgctat?tagcacttgt?ctatatcttt?gtcggtcgta?ttggagcaac?atcacaatca?780

ttatttcctt?ttattgatgg?cagctttacc?cttcatggta?atccagttaa?tggcggtcaa?840

atcttaagtc?atgcttctcg?tttttaccta?ggtggcatcg?gacaagcatt?tctagctgtt?900

gtgattttcc?tggcctgtct?aaccacttca?acaggcttaa?tcacgtcaag?tgctgaatac?960

ttccataaat?tagtgcctgc?tttatctcat?attgcttggg?caactatctt?tactttacta?1020

tcagctttct?tttattttgg?tggcttatca?gtcattatca?actggtcagc?tcctgtttta?1080

ttccttttat?acccattaac?agtcgattta?attttccttg?ttttggcaca?aaaatgcttc?1140

aataatgatc?ctattgtcta?tcgaactaca?attggtctaa?cctttattcc?tgccatattt?1200

gatgcactcc?taacactatc?acaaatgact?ggattatttc?atttaccaga?agccgttgta?1260

acttttttcc?aaaaaactgt?tccactaggg?caattctcaa?tgggatggat?tatctttgct?1320

gctattggtt?ttttaatagg?gcttatacta?agtaaaacga?agaaaagcta?a 1371

<210>8

<211>456

<212>PRT

＜213〉streptococcus pyogenes

<400>8

Met?Lys?Gln?Lys?Asn?Val?Tyr?Ile?Val?Ile?Gly?Phe?Met?Leu?Phe?Ala

1 5 10 15

Leu?Phe?Phe?Gly?Ala?Ala?Asn?Leu?Ile?Tyr?Pro?Ala?Phe?Leu?Gly?Ile

20 25 30

Tyr?Ser?Gly?His?Gln?Ile?Leu?Trp?Ser?Ile?Ile?Gly?Phe?Cys?Leu?Thr

35 40 45

Gly?Val?Ser?Leu?Pro?Leu?Leu?Gly?Val?Ile?Ala?Val?Ala?Lys?Ser?Gly

50 55 60

Ser?Gly?Asp?Val?Glu?Ser?Leu?Ala?Arg?Pro?Ile?Ser?Lys?Trp?Tyr?Ala

65 70 75 80

Ile?Phe?Tyr?Ser?Ser?Ile?Leu?Tyr?Leu?Ser?Ile?Gly?Pro?Phe?Phe?Ala

85 90 95

Ile?Pro?Arg?Thr?Gly?Ala?Thr?Ser?Phe?Ser?Val?Gly?Ile?Ala?Pro?Ile

100 105 110

Leu?Gly?Asp?Asn?Thr?Thr?Asn?Lys?Ala?Ile?Tyr?Ala?Ile?Leu?Phe?Phe

115 120 125

Gly?Leu?Ser?Tyr?Phe?Leu?Ala?Ile?Lys?Pro?Ser?Lys?Leu?Ala?Glu?Asn

130 135 140

Ile?Gly?Lys?Phe?Leu?Thr?Pro?Thr?Leu?Leu?Val?Val?Ile?Ser?Ile?Leu

145 150 155 160

Val?Ile?Ala?Ser?Phe?Val?His?Pro?Ala?Gly?Asn?Tyr?Gly?Asp?Ala?Phe

165 170 175

Asn?Ala?Gly?Val?Gly?Val?Asn?Asn?Ala?Phe?Lys?Asp?Phe?Pro?Phe?Ile

180 185 190

Ala?Gly?Leu?Ile?Gln?Gly?Tyr?Gly?Thr?Met?Asp?Ala?Leu?Ala?Ser?Leu

195 200 205

Val?Phe?Ala?Ile?Leu?Val?Ile?Glu?Ala?Thr?Lys?Gln?Phe?Gly?Ala?Lys

210 215 220

Thr?Asp?Lys?Glu?Met?Thr?Lys?Ile?Thr?Leu?Ile?Ser?Gly?Ala?Ile?Ala

225 230 235 240

Ile?Leu?Leu?Leu?Ala?Leu?Val?Tyr?Ile?Phe?Val?Gly?Arg?Ile?Gly?Ala

245 250 255

Thr?Ser?Gln?Ser?Leu?Phe?Pro?Phe?Ile?Asp?Gly?Ser?Phe?Thr?Leu?His

260 265 270

Gly?Asn?Pro?Val?Asn?Gly?Gly?Gln?Ile?Leu?Ser?His?Ala?Ser?Arg?Phe

275 280 285

Tyr?Leu?Gly?Gly?Ile?Gly?Gln?Ala?Phe?Leu?Ala?Val?Val?Ile?Phe?Leu

290 295 300

Ala?Cys?Leu?Thr?Thr?Ser?Thr?Gly?Leu?Ile?Thr?Ser?Ser?Ala?Glu?Tyr

305 310 315 320

Phe?His?Lys?Leu?Val?Pro?Ala?Leu?Ser?His?Ile?Ala?Trp?Ala?Thr?Ile

325 330 335

Phe?Thr?Leu?Leu?Ser?Ala?Phe?Phe?Tyr?Phe?Gly?Gly?Leu?Ser?Val?Ile

340 345 350

Ile?Asn?Trp?Ser?Ala?Pro?Val?Leu?Phe?Leu?Leu?Tyr?Pro?Leu?Thr?Val

355 360 365

Asp?Leu?Ile?Phe?Leu?Val?Leu?Ala?Gln?Lys?Cys?Phe?Asn?Asn?Asp?Pro

370 375 380

Ile?Val?Tyr?Arg?Thr?Thr?Ile?Gly?Leu?Thr?Phe?Ile?Pro?Ala?Ile?Phe

385 390 395 400

Asp?Ala?Leu?Leu?Thr?Leu?Ser?Gln?Met?Thr?Gly?Leu?Phe?His?Leu?Pro

405 410 415

Glu?Ala?Val?Val?Thr?Phe?Phe?Gln?Lys?Thr?Val?Pro?Leu?Gly?Gln?Phe

420 425 430

Ser?Met?Gly?Trp?Ile?Ile?Phe?Ala?Ala?Ile?Gly?Phe?Leu?Ile?Gly?Leu

435 440 445

Ile?Leu?Ser?Lys?Thr?Lys?Lys?Ser

450 455

<210>9

<211>441

<212>DNA

＜213〉streptococcus pyogenes

<400>9

atggggattt?tagaaaaaaa?acttgataac?ttagtaaata?ctattttatt?aaaagcagaa?60

aatcagcatg?agttattatt?tggagcttgt?caaagtgacg?ttaagcttac?taatacgcaa?120

gaacatattt?taatgttact?atctcagcaa?cgtctcacta?atacagattt?ggctaaggca?180

ttaaatatta?gtcaggcggc?agtaactaag?gctatcaaga?gtttggtcaa?acaagacatg?240

ttagcaggaa?ctaaggatac?ggttgatgct?agggtgactt?attttgaatt?aaccgagtta?300

gctaagccta?ttgcgtcaga?acatacccat?catcatgatg?aaaccttaaa?tgtttacaac?360

cgtttattac?aaaaattctc?cgcgaaagaa?ttagagattg?tagataagtt?tgtaacagtt?420

tttgctgagg?aattagaagg?g 441

<210>10

<211>147

<212>PRT

＜213〉streptococcus pyogenes

<400>10

Met?Gly?Ile?Leu?Glu?Lys?Lys?Leu?Asp?Asn?Leu?Val?Asn?Thr?Ile?Leu

1 5 10 15

Leu?Lys?Ala?Glu?Asn?Gln?His?Glu?Leu?Leu?Phe?Gly?Ala?Cys?Gln?Ser

20 25 30

Asp?Val?Lys?Leu?Thr?Asn?Thr?Gln?Glu?His?Ile?Leu?Met?Leu?Leu?Ser

35 40 45

Gln?Gln?Arg?Leu?Thr?Asn?Thr?Asp?Leu?Ala?Lys?Ala?Leu?Asn?Ile?Ser

50 55 60

Gln?Ala?Ala?Val?Thr?Lys?Ala?Ile?Lys?Ser?Leu?Val?Lys?Gln?Asp?Met

65 70 75 80

Leu?Ala?Gly?Thr?Lys?Asp?Thr?Val?Asp?Ala?Arg?Val?Thr?Tyr?Phe?Glu

85 90 95

Leu?Thr?Glu?Leu?Ala?Lys?Pro?Ile?Ala?Ser?Glu?His?Thr?His?His?His

100 105 110

Asp?Glu?Thr?Leu?Asn?Val?Tyr?Asn?Arg?Leu?Leu?Gln?Lys?Phe?Ser?Ala

115 120 125

Lys?Glu?Leu?Glu?Ile?Val?Asp?Lys?Phe?Val?Thr?Val?Phe?Ala?Glu?Glu

130 135 140

Leu?Glu?Gly

145

<210>11

<211>815

<212>DNA

＜213〉streptococcus pyogenes

<400>11

atgcttgata?ttttattcta?tgatttcatg?caacgggcgg?taatggcggt?agttgccatt?60

agtatttttg?ctccgatttt?aggtattttc?cttattttac?gtcgtcaaag?tttgatgagc?120

gataccctta?gtcatgtttc?tttggctggg?gtagcgcttg?gggtagtcct?tggtatttca?180

ccaaccatca?ctactattat?tgttgtggtt?ttagctgcta?ttttgttaga?atacctgcgt?240

gtagtttaca?aacactacat?ggagatttca?acggcgattt?tgatgtcact?tggcttggcc?300

ctatctctga?ttattatgag?taagtcgcat?agttcatcaa?gcatgagttt?agaacaatac?360

ctttttggat?cgatcatcac?gattagtatg?gaacaagttg?tcgccttgtt?tgctattgct?420

gcgattattt?taatcttgac?cgttctcttc?attagaccga?tgtacattct?gacctttgat?480

gaagatactg?cttttgtaga?tggtttgccc?gttcgcttga?tgtctgttct?attcaatatc?540

gtcactgggg?ttgctattgc?tttgaccatt?ccagcagcag?gagcactttt?ggtttctacc?600

attatggtct?tgccagcaag?tatcgcaatg?agattgggta?aaaactttaa?aacagttatc?660

ttactgggaa?ttgtcatcgg?ttttagcggt?atgttatctg?gtattttctt?atcttatttc?720

tttgaaacgc?cagctagtgc?cactattacc?atgattttca?ttagtatttt?cctcttagtt?780

agtctaggtg?gaatgcttaa?aaaacggtta?tttaa 815

<210>12

<211>239

<212>PRT

＜213〉streptococcus pyogenes

<400>12

Met?Arg?Tyr?Ile?Ser?Val?Lys?Asn?Leu?Ser?Phe?Gln?Tyr?Glu?Ser?Glu

1 5 10 15

Pro?Val?Leu?Glu?Gly?Ile?Thr?Tyr?His?Leu?Asp?Ser?Gly?Glu?Phe?Val

20 25 30

Thr?Met?Thr?Gly?Glu?Asn?Gly?Ala?Ala?Lys?Ser?Thr?Leu?Ile?Lys?Ala

35 40 45

Thr?Leu?Gly?Ile?Leu?Gln?Pro?Lys?Ala?Gly?Arg?Val?Thr?Ile?Ala?Lys

50 55 60

Lys?Asn?Lys?Asp?Gly?Lys?Gln?Leu?Arg?Ile?Ala?Tyr?Leu?Pro?Gln?Gln

65 70 75 80

Val?Ala?Ser?Phe?Asn?Ala?Gly?Phe?Pro?Ser?Thr?Val?Tyr?Glu?Phe?Val

85 90 95

Lys?Ser?Gly?Arg?Tyr?Pro?Arg?Ser?Gly?Trp?Phe?Arg?His?Leu?Asn?Lys

100 105 110

His?Asp?Glu?Glu?His?Val?Gln?Ala?Ser?Leu?Glu?Ala?Val?Gly?Met?Trp

115 120 125

Glu?Asn?Arg?His?Lys?Arg?Ile?Gly?Ser?Leu?Ser?Gly?Gly?Gln?Lys?Gln

130 135 140

Arg?Val?Val?Ile?Ala?Arg?Met?Phe?Ala?Ser?Asp?Pro?Asp?Ile?Phe?Val

145 150 155 160

Leu?Asp?Glu?Pro?Thr?Thr?Gly?Met?Asp?Ser?Gly?Thr?Thr?Asp?Thr?Phe

165 170 175

Tyr?Glu?Leu?Met?His?His?Ser?Ala?His?Gln?His?Gly?Lys?Ser?Val?Leu

180 185 190

Met?Ile?Thr?His?Asp?Pro?Glu?Glu?Val?Lys?Ala?Tyr?Ala?Asp?Arg?Asn

195 200 205

Ile?His?Leu?Val?Arg?Asn?Gln?Lys?Leu?Pro?Trp?Arg?Cys?Phe?Asn?Ile

210 215 220

His?Glu?Ala?Glu?Thr?Asp?Asp?Glu?Lys?Gly?Gly?His?Gly?His?Ala

225 230 235

<210>13

<211>681

<212>DNA

＜213〉streptococcus pyogenes

<400>13

atgtattcta?taaaatgtga?tgataataaa?gccatgccaa?gagaacgttt?gatgcgacta?60

ggagcagagt?ctctaagtaa?tcaagaatta?ttagcaattt?tattacgaac?aggtaataaa?120

gaaaagcatg?tcttagagct?gtcatcctat?cttttatcgc?atttagacag?tctggcagat?180

tttaaaaaga?tgtctttgca?agaattacaa?catttggcag?gtataggaaa?agttaaagcg?240

attgaaatta?aagctatgat?tgagttggtt?tcccgaattt?tagcgacaga?taagacatta?300

actgatagcg?tattaaccag?tgttcaggtc?gctgaaaaaa?tgatggcagc?tttaggagat?360

aaaaaacaag?agcatttagt?cgtattgtat?ttagataatc?aaaatcgtat?tattgaagaa?420

aaaactattt?ttattgggac?tgtccgacgt?tcacttgcag?aaccaagaga?aattttatac?480

tatgcctgta?aaaatatggc?gactagtctc?attgttattc?ataatcatcc?ttcaggaaat?540

attgaaccta?gttctaacga?ttattgcttt?actgaaaaaa?taaaacgatc?atgtgaagat?600

ttaggcatta?tctgtctaga?tcacattatc?gttagctata?aagattatta?tagttttcga?660

gaaaaatcaa?ccctttttta?a 681

<210>14

<211>226

<212>PRT

＜213〉streptococcus pyogenes

<400>14

Met?Tyr?Ser?Ile?Lys?Cys?Asp?Asp?Asn?Lys?Ala?Met?Pro?Arg?Glu?Arg

1 5 10 15

Leu?Met?Arg?Leu?Gly?Ala?Glu?Ser?Leu?Ser?Asn?Gln?Glu?Leu?Leu?Ala

20 25 30

Ile?Leu?Leu?Arg?Thr?Gly?Asn?Lys?Glu?Lys?His?Val?Leu?Glu?Leu?Ser

35 40 45

Ser?Tyr?Leu?Leu?Ser?His?Leu?Asp?Ser?Leu?Ala?Asp?Phe?Lys?Lys?Met

50 55 60

Ser?Leu?Gln?Glu?Leu?Gln?His?Leu?Ala?Gly?Ile?Gly?Lys?Val?Lys?Ala

65 70 75 80

Ile?Glu?Ile?Lys?Ala?Met?Ile?Glu?Leu?Val?Ser?Arg?Ile?Leu?Ala?Thr

85 90 95

Asp?Lys?Thr?Leu?Thr?Asp?Ser?Val?Leu?Thr?Ser?Val?Gln?Val?Ala?Glu

100 105 110

Lys?Met?Met?Ala?Ala?Leu?Gly?Asp?Lys?Lys?Gln?Glu?His?Leu?Val?Val

115 120 125

Leu?Tyr?Leu?Asp?Asn?Gln?Asn?Arg?Ile?Ile?Glu?Glu?Lys?Thr?Ile?Phe

130 135 140

Ile?Gly?Thr?Val?Arg?Arg?Ser?Leu?Ala?Glu?Pro?Arg?Glu?Ile?Leu?Tyr

145 150 155 160

Tyr?Ala?Cys?Lys?Asn?Met?Ala?Thr?Ser?Leu?Ile?Val?Ile?His?Asn?His

165 170 175

Pro?Ser?Gly?Asn?Ile?Glu?Pro?Ser?Ser?Asn?Asp?Tyr?Cys?Phe?Thr?Glu

180 185 190

Lys?Ile?Lys?Arg?Ser?Cys?Glu?Asp?Leu?Gly?Ile?Ile?Cys?Leu?Asp?His

195 200 205

Ile?Ile?Val?Ser?Tyr?Lys?Asp?Tyr?Tyr?Ser?Phe?Arg?Glu?Lys?Ser?Thr

210 215 220

Leu?Phe

225

<210>15

<211>410

<212>DNA

＜213〉streptococcus pyogenes

<400>15

atgatgttac?gaaaatttaa?aattaccatt?gacggtaaag?agtatcttgt?agaaatggaa?60

gaaatcggtg?ctcctgccca?agcagctgcc?cccgctcaac?caatatccac?tccagtacct?120

gtccctactg?aagcaagccc?acaagtggag?gaagcgcaag?caccacagcc?agtagctgca?180

gcgggagcgg?atgccattcc?atcaccaatg?cctggaacca?tcttaaaagt?cttagtagca?240

gtgggagacc?aagtaactga?aaatcagcca?ctattgattt?tagaagccat?gaagatggaa?300

aatgagattg?tggcctcatc?agcaggaacc?attacagcta?tccacgtcgg?tccaggtcaa?360

gtggtcaatc?ctggtgatgg?tctcattaca?attggttaat?agtactataa 410

<210>16

<211>134

<212>PRT

＜213〉streptococcus pyogenes

<400>16

Met?Met?Leu?Arg?Lys?Phe?Lys?Ile?Thr?Ile?Asp?Gly?Lys?Glu?Tyr?Leu

1 5 10 15

Val?Glu?Met?Glu?Glu?Ile?Gly?Ala?Pro?Ala?Gln?Ala?Ala?Ala?Pro?Ala

20 25 30

Gln?Pro?Ile?Ser?Thr?Pro?Val?Pro?Val?Pro?Thr?Glu?Ala?Ser?Pro?Gln

35 40 45

Val?Glu?Glu?Ala?Gln?Ala?Pro?Gln?Pro?Val?Ala?Ala?Ala?Gly?Ala?Asp

50 55 60

Ala?Ile?Pro?Ser?Pro?Met?Pro?Gly?Thr?Ile?Leu?Lys?Val?Leu?Val?Ala

65 70 75 80

Val?Gly?Asp?Gln?Val?Thr?Glu?Asn?Gln?Pro?Leu?Leu?Ile?Leu?Glu?Ala

85 90 95

Met?Lys?Met?Glu?Asn?Glu?Ile?Val?Ala?Ser?Ser?Ala?Gly?Thr?Ile?Thr

100 105 110

Ala?Ile?His?Val?Gly?Pro?Gly?Gln?Val?Val?Asn?Pro?Gly?Asp?Gly?Leu

115 120 125

Ile?Thr?Ile?Gly?Tyr?Tyr

130

<210>17

<211>1533

<212>DNA

＜213〉streptococcus pyogenes

<400>17

atggtaacaa?ataaactcgg?tagagatatt?cctcaaccat?acgccgacca?atacggtgtc?60

tttgaaggag?aactcgcaaa?tatcaaacag?tatgacgaat?caagtcgccg?tattaaacca?120

gttaagcctg?gagattctaa?attactagga?tctgttcgtg?aagcgattga?aaaaacaggt?180

ttaacagatg?gcatgaccat?ttctttccac?caccatttcc?gtgaagggga?tttcattatg?240

aatatggtct?tggaggaaat?tgccaaaatg?ggcattaaaa?acctgtctat?tgccccaagt?300

tccatcgcta?atgtacatga?gcctttgatt?gatcacatca?aaaatggtgt?ggtcactaac?360

atcacttcat?ctggtcttcg?tgacaaagta?ggggcagcca?tctcagaagg?actaatggaa?420

aatcctgtgg?ttattcgctc?ccatggtggt?cgcgctcgtg?ccattgctag?tggggatatc?480

catattgatg?ttgcctttct?gggcgcccca?agttcagatg?cttacggaaa?tgttaatggg?540

acaaaaggga?aagcaacctg?tggttctttg?ggctatgcca?tgattgatgc?caaatatgcg?600

gatcaggtgg?ttatcttgac?agataatttg?gttccttatc?ctaatacccc?aatcagcatt?660

cctcaaacag?atgttgacta?tgtggtaaca?gtggatgcta?tcggagatcc?tcaaggaatc?720

gccaaagggg?caactcgttt?tacgaaaaat?cctaaagaac?tcttaattgc?agaatacgcc?780

gctaaagtaa?tcaccaactc?tccttatttt?aaagaaggat?tctctttcca?aactggaaca?840

ggtggcgctt?ctttggcagt?aacccgtttc?atgcgtgaag?ctatgattaa?agaaaatatc?900

aaagctagct?ttgctcttgg?tggtattacc?aatgctatgg?tggagttgct?cgaagaagaa?960

ctggttgaaa?aaattcttga?tgtccaagac?tttgatcacc?catcagctgt?ttctcttggt?1020

aagcatgctg?aacattatga?aattgatgcc?aacatgtatg?cctcaccttt?gagcaagggt?1080

gctgttatca?atcaattaga?cacttgtatt?ttatcagccc?ttgaagttga?tactaacttc?1140

aatgttaatg?tgatgacagg?atctgacggc?gtgattcgtg?gcgcttctgg?aggacactgc?1200

gacactgcct?ttgcggctaa?gatgagtttg?gttatttcac?cacttatccg?cggacgcatc?1260

ccaacttttg?tagatgaggt?taatacggtc?attacaccag?gaacaagtgt?cgatgtgatc?1320

gtcacagaag?tgggaattgc?cattaaccca?aatcgtcaag?acttagtaga?ccatttcaaa?1380

tccctcaatg?tgccacaatt?tagtattgag?gagctaaaag?agaaggctta?cgccatcgtt?1440

ggcacacctg?agcgtattca?atatggtgat?aaggttgttg?ctcttattga?ataccgtgac?1500

ggtagcctca?tggatgtggt?ttacaatgtg?taa 1533

<210>18

<211>510

<212>PRT

＜213〉streptococcus pyogenes

<400>18

Met?Val?Thr?Asn?Lys?Leu?Gly?Arg?Asp?Ile?Pro?Gln?Pro?Tyr?Ala?Asp

1 5 10 15

Gln?Tyr?Gly?Val?Phe?Glu?Gly?Glu?Leu?Ala?Asn?Ile?Lys?Gln?Tyr?Asp

20 25 30

Glu?Ser?Ser?Arg?Arg?Ile?Lys?Pro?Val?Lys?Pro?Gly?Asp?Ser?Lys?Leu

35 40 45

Leu?Gly?Ser?Val?Arg?Glu?Ala?Ile?Glu?Lys?Thr?Gly?Leu?Thr?Asp?Gly

50 55 60

Met?Thr?Ile?Ser?Phe?His?His?His?Phe?Arg?Glu?Gly?Asp?Phe?Ile?Met

65 70 75 80

Asn?Met?Val?Leu?Glu?Glu?Ile?Ala?Lys?Met?Gly?Ile?Lys?Asn?Leu?Ser

85 90 95

Ile?Ala?Pro?Ser?Ser?Ile?Ala?Asn?Val?His?Glu?Pro?Leu?Ile?Asp?His

100 105 110

Ile?Lys?Asn?Gly?Val?Val?Thr?Asn?Ile?Thr?Ser?Ser?Gly?Leu?Arg?Asp

115 120 125

Lys?Val?Gly?Ala?Ala?Ile?Ser?Glu?Gly?Leu?Met?Glu?Asn?Pro?Val?Val

130 135 140

Ile?Arg?Ser?His?Gly?Gly?Arg?Ala?Arg?Ala?Ile?Ala?Ser?Gly?Asp?Ile

145 150 155 160

His?Ile?Asp?Val?Ala?Phe?Leu?Gly?Ala?Pro?Ser?Ser?Asp?Ala?Tyr?Gly

165 170 175

Asn?Val?Asn?Gly?Thr?Lys?Gly?Lys?Ala?Thr?Cys?Gly?Ser?Leu?Gly?Tyr

180 185 190

Ala?Met?Ile?Asp?Ala?Lys?Tyr?Ala?Asp?Gln?Val?Val?Ile?Leu?Thr?Asp

195 200 205

Asn?Leu?Val?Pro?Tyr?Pro?Asn?Thr?Pro?Ile?Ser?Ile?Pro?Gln?Thr?Asp

210 215 220

Val?Asp?Tyr?Val?Val?Thr?Val?Asp?Ala?Ile?Gly?Asp?Pro?Gln?Gly?Ile

225 230 235 240

Ala?Lys?Gly?Ala?Thr?Arg?Phe?Thr?Lys?Asn?Pro?Lys?Glu?Leu?Leu?Ile

245 250 255

Ala?Glu?Tyr?Ala?Ala?Lys?Val?Ile?Thr?Asn?Ser?Pro?Tyr?Phe?Lys?Glu

260 265 270

Gly?Phe?Ser?Phe?Gln?Thr?Gly?Thr?Gly?Gly?Ala?Ser?Leu?Ala?Val?Thr

275 280 285

Arg?Phe?Met?Arg?Glu?Ala?Met?Ile?Lys?Glu?Asn?Ile?Lys?Ala?Ser?Phe

290 295 300

Ala?Leu?Gly?Gly?Ile?Thr?Asn?Ala?Met?Val?Glu?Leu?Leu?Glu?Glu?Glu

305 310 315 320

Leu?Val?Glu?Lys?Ile?Leu?Asp?Val?Gln?Asp?Phe?Asp?His?Pro?Ser?Ala

325 330 335

Val?Ser?Leu?Gly?Lys?His?Ala?Glu?His?Tyr?Glu?Ile?Asp?Ala?Asn?Met

340 345 350

Tyr?Ala?Ser?Pro?Leu?Ser?Lys?Gly?Ala?Val?Ile?Asn?Gln?Leu?Asp?Thr

355 360 365

Cys?Ile?Leu?Ser?Ala?Leu?Glu?Val?Asp?Thr?Asn?Phe?Asn?Val?Asn?Val

370 375 380

Met?Thr?Gly?Ser?Asp?Gly?Val?Ile?Arg?Gly?Ala?Ser?Gly?Gly?His?Cys

385 390 395 400

Asp?Thr?Ala?Phe?Ala?Ala?Lys?Met?Ser?Leu?Val?Ile?Ser?Pro?Leu?Ile

405 410 415

Arg?Gly?Arg?Ile?Pro?Thr?Phe?Val?Asp?Glu?Val?Asn?Thr?Val?Ile?Thr

420 425 430

Pro?Gly?Thr?Ser?Val?Asp?Val?Ile?Val?Thr?Glu?Val?Gly?Ile?Ala?Ile

435 440 445

Asn?Pro?Asn?Arg?Gln?Asp?Leu?Val?Asp?His?Phe?Lys?Ser?Leu?Asn?Val

450 455 460

Pro?Gln?Phe?Ser?Ile?Glu?Glu?Leu?Lys?Glu?Lys?Ala?Tyr?Ala?Ile?Val

465 470 475 480

Gly?Thr?Pro?Glu?Arg?Ile?Gln?Tyr?Gly?Asp?Lys?Val?Val?Ala?Leu?Ile

485 490 495

Glu?Tyr?Arg?Asp?Gly?Ser?Leu?Met?Asp?Val?Val?Tyr?Asn?Val

500 505 510

<210>19

<211>888

<212>DNA

＜213〉streptococcus pyogenes

<400>19

atggaacgtt?taagaagaac?aatgatgttt?gtgcctggtg?ccaatgcagc?catgcttcga?60

gatgctcctt?tatttggcgc?tgattcaatc?atgtttgact?tggaagattc?tgtttcactc?120

aaagaaaaag?acacctctag?agctctcgtt?cattttgcgc?ttaaaacttt?tgattattca?180

agcgttgaaa?cggttgttcg?tgtgaatggt?cttgattctt?gtggggcttt?agacatcgaa?240

gctgttgttt?tggcaggtgt?caacgtgatt?cgtcttccaa?aaacagagac?tgctcaagat?300

attattgatg?tggaggctgt?tattgaacgt?gtcgaacgcg?agaacagcat?tgaagttggt?360

cgcacacgta?tgatggcagc?cattgaatca?gccgaaggtg?tcctaaatgc?tcgtgagatt?420

gccaaagctt?ctaagcgctt?gattggtatt?gctcttggag?cagaagacta?tgtcacgaat?480

atgaaaacgc?gtcgttaccc?agatggtcaa?gaattattct?ttgctcgtag?catgatttta?540

cacgctgctc?gtgctgctgg?aattgctgcc?attgatactg?tttattctga?tgtcaataat?600

accgaagggt?tccaaaacga?agttcgcatg?atcaaacagt?taggatttga?tggtaaatcg?660

gtcatcaacc?ctcgccaaat?tcctctggtc?aatgagattt?ataccccaac?aaaaaaagaa?720

attgaccatg?ccaaacaagt?catctgggca?attcgtgaag?ctgaaagcaa?aggctctggc?780

gttatttcct?taaatggaaa?aatggttgat?aaaccaatcg?ttgaacgtgc?agaacgtgtg?840

attgccctag?caacagcagc?aggtgtttta?tctgaggagg?atatttaa 888

<210>20

<211>295

<212>PRT

＜213〉streptococcus pyogenes

<400>20

Met?Glu?Arg?Leu?Arg?Arg?Thr?Met?Met?Phe?Val?Pro?Gly?Ala?Asn?Ala

1 5 10 15

Ala?Met?Leu?Arg?Asp?Ala?Pro?Leu?Phe?Gly?Ala?Asp?Ser?Ile?Met?Phe

20 25 30

Asp?Leu?Glu?Asp?Ser?Val?Ser?Leu?Lys?Glu?Lys?Asp?Thr?Ser?Arg?Ala

35 40 45

Leu?Val?His?Phe?Ala?Leu?Lys?Thr?Phe?Asp?Tyr?Ser?Ser?Val?Glu?Thr

50 55 60

Val?Val?Arg?Val?Asn?Gly?Leu?Asp?Ser?Cys?Gly?Ala?Leu?Asp?Ile?Glu

65 70 75 80

Ala?Val?Val?Leu?Ala?Gly?Val?Asn?Val?Ile?Arg?Leu?Pro?Lys?Thr?Glu

85 90 95

Thr?Ala?Gln?Asp?Ile?Ile?Asp?Val?Glu?Ala?Val?Ile?Glu?Arg?Val?Glu

100 105 110

Arg?Glu?Asn?Ser?Ile?Glu?Val?Gly?Arg?Thr?Arg?Met?Met?Ala?Ala?Ile

115 120 125

Glu?Ser?Alg?Glu?Gly?Val?Leu?Asn?Ala?Arg?Glu?Ile?Ala?Lys?Ala?Ser

130 135 140

Lys?Arg?Leu?Ile?Gly?Ile?Ala?Leu?Gly?Ala?Glu?Asp?Tyr?Val?Thr?Asn

145 150 155 160

Met?Lys?Thr?Arg?Arg?Tyr?Pro?Asp?Gly?Gln?Glu?Leu?Phe?Phe?Ala?Arg

165 170 175

Ser?Met?Ile?Leu?His?Ala?Ala?Arg?Ala?Ala?Gly?Ile?Ala?Ala?Ile?Asp

180 185 190

Thr?Val?Tyr?Ser?Asp?Val?Asn?Asn?Thr?Glu?Gly?Phe?Gln?Asn?Glu?Val

195 200 205

Arg?Met?Ile?Lys?Gln?Leu?Gly?Phe?Asp?Gly?Lys?Ser?Val?Ile?Asn?Pro

210 215 220

Arg?Gln?Ile?Pro?Leu?Val?Asn?Glu?Ile?Tyr?Thr?Pro?Thr?Lys?Lys?Glu

225 230 235 240

Ile?Asp?His?Ala?Lys?Gln?Val?Ile?Trp?Ala?Ile?Arg?Glu?Ala?Glu?Ser

245 250 255

Lys?Gly?Ser?Gly?Val?Ile?Ser?Leu?Asn?Gly?Lys?Met?Val?Asp?Lys?Pro

260 265 270

Ile?Val?Glu?Arg?Ala?Glu?Arg?Val?Ile?Ala?Leu?Ala?Thr?Ala?Ala?Gly

275 280 285

Val?Leu?Ser?Glu?Glu?Asp?Ile

290 295

<210>21

<211>309

<212>DNA

＜213〉streptococcus pyogenes

<400>21

atggatatta?aacaaactgc?cgttgctggt?tcacttgaat?caagtgacct?aatgattaca?60

gtatccccta?atgacgagca?aaccattaca?ataaccctag?acagtagtgt?tgaaaaacaa?120

tttggcaatc?acattcgtca?actcattcat?caaaccctag?tgaatttgaa?ggtgacggct?180

gctaaagtag?aggctgttga?taaaggtgca?ttagattgca?ccattcaagc?gagaaccatc?240

gcagctgttc?atcgcgctgc?tggtattgac?caatacgatt?ggaaggagat?tgactcatgg?300

aacgtttaa 309

<210>22

<211>102

<212>PRT

＜213〉streptococcus pyogenes

<400>22

Met?Asp?Ile?Lys?Gln?Thr?Ala?Val?Ala?Gly?Ser?Leu?Glu?Ser?Ser?Asp

1 5 10 15

Leu?Met?Ile?Thr?Val?Ser?Pro?Asn?Asp?Glu?Gln?Thr?Ile?Thr?Ile?Thr

20 25 30

Leu?Asp?Ser?Ser?Val?Glu?Lys?Gln?Phe?Gly?Asn?His?Ile?Arg?Gln?Leu

35 40 45

Ile?His?Gln?Thr?Leu?Val?Ash?Leu?Lys?Val?Thr?Ala?Ala?Lys?Val?Glu

50 55 60

Ala?Val?Asp?Lys?Gly?Ala?Leu?Asp?Cys?Thr?Ile?Gln?Ala?Arg?Thr?Ile

65 70 75 80

Ala?Ala?Val?His?Arg?Ala?Ala?Gly?Ile?Asp?Gln?Tyr?Asp?Trp?Lys?Glu

85 90 95

Ile?Asp?Ser?Trp?Asn?Val

100

<210>23

<211>1053

<212>DNA

＜213〉streptococcus pyogenes

<400>23

atgccttact?acactatttc?aaaagtcttt?ccttcagata?aaacgacaat?ggctagtgta?60

aagaacctgc?ttcatcaaga?aggtattcgt?ttggatgctc?atttagatta?tacttgtgcc?120

atcatgaatg?cgcaaaatga?tgtgattgct?accggttctt?actttggaaa?tagcttgcgc?180

tgtctttgcg?tatccagtgc?ctatcaaggt?gaaggacttc?ttaataggat?tgtgagtcat?240

ctcattgatg?aagaatatgc?ccttggcaac?tatcatcttt?ttgtttatac?gaagacctct?300

tctgctgctt?ttttcaaaga?tcttggtttt?actgaaattg?tccatatcga?caatcacatc?360

agtttcttgg?aaaataaaaa?aactggtttt?caagattatc?tgatgacact?taataagcct?420

gagcaaacac?ctggtaaagt?cgctgctatt?gttatcaacg?ctaacccctt?taccttaggg?480

catcaatttt?tagtagagaa?agctgcaaga?gaaaatgatt?gggttcatct?gtttatggtc?540

agtgaagacc?gcagtctaat?tcctttttcg?gtgagaaaga?ggttgattca?agaaggtcta?600

gctcatcttg?ataatgtcat?atatcatgaa?acaggtcctt?atttgattag?ccaagcgact?660

ttcccagcct?atttccaaaa?agaagacaat?gacgtgatta?agagccaagc?tttgctggat?720

actgctattt?ttctaaagat?tgctcaaacc?ttacagatta?caaaaagata?cgttggagaa?780

gagccaacca?gccgagtaac?tgctatttac?aatgaaatta?tggcagagca?gttgcagcaa?840

gctggtatcc?tcctagatat?tttgccaagg?aaagctatca?atcagcagca?agatcctatc?900

agtgcctcaa?cggctaggca?ggcattgaaa?gataatgatt?gggaccttct?ggcaaaactc?960

cttcccaaaa?cgtctttaga?ttatttttgt?tcgctaaaag?cccaacccat?tattaaaaag?1020

atacaagcca?cttcatctgt?caaacattac?taa 1053

<210>24

<211>350

<212>PRT

＜213〉streptococcus pyogenes

<400>24

Met?Pro?Tyr?Tyr?Thr?Ile?Ser?Lys?Val?Phe?Pro?Ser?Asp?Lys?Thr?Thr

1 5 10 15

Met?Ala?Ser?Val?Lys?Asn?Leu?Leu?His?Gln?Glu?Gly?Ile?Arg?Leu?Asp

20 25 30

Ala?His?Leu?Asp?Tyr?Thr?Cys?Ala?Ile?Met?Asn?Ala?Gln?Asn?Asp?Val

35 40 45

Ile?Ala?Thr?Gly?Ser?Tyr?Phe?Gly?Asn?Ser?Leu?Arg?Cys?Leu?Cys?Val

50 55 60

Ser?Ser?Ala?Tyr?Gln?Gly?Glu?Gly?Leu?Leu?Asn?Arg?Ile?Val?Ser?His

65 70 75 80

Leu?Ile?Asp?Glu?Glu?Tyr?Ala?Leu?Gly?Asn?Tyr?His?Leu?Phe?Val?Tyr

85 90 95

Thr?Lys?Thr?Ser?Ser?Ala?Ala?Phe?Phe?Lys?Asp?Leu?Gly?Phe?Thr?Glu

100 105 110

Ile?Val?His?Ile?Asp?Asn?His?Ile?Ser?Phe?Leu?Glu?Asn?Lys?Lys?Thr

115 120 125

Gly?Phe?Gln?Asp?Tyr?Leu?Met?Thr?Leu?Asn?Lys?Pro?Glu?Gln?Thr?Pro

130 135 140

Gly?Lys?Val?Ala?Ala?Ile?Val?Ile?Asn?Ala?Asn?Pro?Phe?Thr?Leu?Gly

145 150 155 160

His?Gln?Phe?Leu?Val?Glu?Lys?Ala?Ala?Arg?Glu?Asn?Asp?Trp?Val?His

165 170 175

Leu?Phe?Met?Val?Ser?Glu?Asp?Arg?Ser?Leu?Ile?Pro?Phe?Ser?Val?Arg

180 185 190

Lys?Arg?Leu?Ile?Gln?Glu?Gly?Leu?Ala?His?Leu?Asp?Asn?Val?Ile?Tyr

195 200 205

His?Glu?Thr?Gly?Pro?Tyr?Leu?Ile?Ser?Gln?Ala?Thr?Phe?Pro?Ala?Tyr

210 215 220

Phe?Gln?Lys?Glu?Asp?Asn?Asp?Val?Ile?Lys?Ser?Gln?Ala?Leu?Leu?Asp

225 230 235 240

Thr?Ala?Ile?Phe?Leu?Lys?Ile?Ala?Gln?Thr?Leu?Gln?Ile?Thr?Lys?Arg

245 250 255

Tyr?Val?Gly?Glu?Glu?Pro?Thr?Ser?Arg?Val?Thr?Ala?Ile?Tyr?Asn?Glu

260 265 270

Ile?Met?Ala?Glu?Gln?Leu?Gln?Gln?Ala?Gly?Ile?Leu?Leu?Asp?Ile?Leu

275 280 285

Pro?Arg?Lys?Ala?Ile?Asn?Gln?Gln?Gln?Asp?Pro?Ile?Ser?Ala?Ser?Thr

290 295 300

Ala?Arg?Gln?Ala?Leu?Lys?Asp?Asn?Asp?Trp?Asp?Leu?Leu?Ala?Lys?Leu

305 310 315 320

Leu?Pro?Lys?Thr?Ser?Leu?Asp?Tyr?Phe?Cys?Ser?Leu?Lys?Ala?Gln?Pro

325 330 335

Ile?Ile?Lys?Lys?Ile?Gln?Ala?Thr?Ser?Ser?Val?Lys?His?Tyr

340 345 350

<210>25

<21l>642

<212>DNA

＜213〉streptococcus pyogenes

<400>25

atggtgataa?ggttgttgct?cttattgaat?accgtgacgg?tagcctcatg?gatgtggttt?60

acaatgtgta?aagacactta?tttttcaggc?gaagctatcc?aacttagtga?tatgttaaga?120

gcccgcgaag?aaagagctct?gcgtcagctg?catttattaa?aggagtaccc?agaaggtagc?180

ttattatcgg?tcaccatgaa?tatccctgga?ccaattaaaa?cctctcctaa?acttcttgaa?240

gcttttgata?tagtgattaa?ggccattcaa?actgccttag?ctgacgataa?gatttgttac?300

cagttgcgat?tactgcctac?aacgggttat?gagtattacc?tcatcacaag?tctacctagc?360

cgcgacctga?agttaaaaat?gatagcctta?gagacagagt?tgccaatagg?tcgtctcatg?420

gatttagatg?tcttggtctt?gcaaaatgat?ctgcctcatt?caattagcag?aaccgtatta?480

ggaggctccc?ctaggcaatg?ttttatctgt?tctaaagagg?ccaaagtctg?cggtcgccta?540

cgtaagcaca?gtgtcgagga?gatgcagact?gctatttcaa?aattactcca?ttcatttttc?600

aataaagaca?accaatcatc?gtcatcagat?aagacaggtt?ga 642

<210>26

<211>213

<212>PRT

＜213〉streptococcus pyogenes

<400>26

Met?Val?Ile?Arg?Leu?Leu?Leu?Leu?Leu?Asn?Thr?Val?Thr?Val?Ala?Ser

1 5 10 15

Trp?Met?Trp?Phe?Thr?Met?Cys?Lys?Asp?Thr?Tyr?Phe?Ser?Gly?Glu?Ala

20 25 30

Ile?Gln?Leu?Ser?Asp?Met?Leu?Arg?Ala?Arg?Glu?Glu?Arg?Ala?Leu?Arg

35 40 45

Gln?Leu?His?Leu?Leu?Lys?Glu?Tyr?Pro?Glu?Gly?Ser?Leu?Leu?Ser?Val

50 55 60

Thr?Met?Asn?Ile?Pro?Gly?Pro?Ile?Lys?Thr?Ser?Pro?Lys?Leu?Leu?Glu

65 70 75 80

Ala?Phe?Asp?Ile?Val?Ile?Lys?Ala?Ile?Gln?Thr?Ala?Leu?Ala?Asp?Asp

85 90 95

Lys?Ile?Cys?Tyr?Gln?Leu?Arg?Leu?Leu?Pro?Thr?Thr?Gly?Tyr?Glu?Tyr

100 105 110

Tyr?Leu?Ile?Thr?Ser?Leu?Pro?Ser?Arg?Asp?Leu?Lys?Leu?Lys?Met?Ile

115 120 125

Ala?Leu?Glu?Thr?Glu?Leu?Pro?Ile?Gly?Arg?Leu?Met?Asp?Leu?Asp?Val

130 135 140

Leu?Val?Leu?Gln?Asn?Asp?Leu?Pro?His?Ser?Ile?Ser?Arg?Thr?Val?Leu

145 150 155 160

Gly?Gly?Ser?Pro?Arg?Gln?Cys?Phe?Ile?Cys?Ser?Lys?Glu?Ala?Lys?Val

165 170 175

Cys?Gly?Arg?Leu?Arg?Lys?His?Ser?Val?Glu?Glu?Met?Gln?Thr?Ala?Ile

180 185 190

Ser?Lys?Leu?Leu?His?Ser?Phe?Phe?Asn?Lys?Asp?Asn?Gln?Ser?Ser?Ser

195 200 205

Ser?Asp?Lys?Thr?Gly

210

<210>27

<211>1395

<212>DNA

＜213〉streptococcus pyogenes

<400>27

atgacgaaaa?tccgtataac?ggaaacagtt?ttacgtgatg?gtcaacaaag?tcagattgcc?60

acacgtatga?caaccaagga?aatgattcct?attctcgaaa?cgctagacaa?tgctggttat?120

catgcccttg?aaatgtgggg?aggagcaacc?tttgattcct?gcctacgctt?tttaaatgaa?180

gacccttggg?aacggctaag?agccatccgt?aaagcagtga?aaaagactaa?acttcagatg?240

ctcttacgcg?gacaaaacct?tcttggatat?cgcaattatg?ctgacgatgt?ggtcagatcc?300

tttattcaaa?aatccattga?aaatgggatt?gatattgtcc?gtattttcga?tgctttaaac?360

gacccacgca?acttgcaaac?agctgtttca?gcgactaaaa?aatttggagg?gcatgctcaa?420

gttgccattt?cttacacgac?aagtccggta?cataccattg?actactttgt?tgaattggcg?480

aaagcttacc?aagctatcgg?agcggactcc?atttgtatca?aagacatggc?tggtgtctta?540

actcctgaaa?taggttacca?attggtcaaa?tgtatcaagg?agaatacaac?tatccctctt?600

gaggttcata?cccatgctac?cagtggtatt?tcagaaatga?cttacttaaa?agtagcagaa?660

gcaggagctg?atattattga?tacggcgatt?tcctcttttt?cagggggaac?cagtcagcct?720

gccacagagt?caatggcgat?tgccttgacg?gatttaggct?ttgacacagg?cttggatatg?780

caagaggtgg?ccaaagttgc?agaatatttc?aacactattc?gtgaccacta?tcgagaaata?840

gggattttaa?atcctaaagt?caaagatact?gagccaaaaa?cattgattta?ccaagttcca?900

ggtggcatgc?tatcaaacct?attgagccaa?ttaaccgagc?aaggcctaac?tgataaatac?960

gaagaagtct?tagcagaagt?acctaaagta?agggctgatc?ttgggtatcc?gccactcgta?1020

acgccattat?cacaaatggt?tggcacacag?gccttgatga?atatcatctc?aggggaacgt?1080

tacaaggtag?ttccaaatga?aatcaaagac?tatgttcgag?ggctatacgg?tcaatcacca?1140

gcgccattag?cagaaggtat?caaagagaaa?atcattggtg?acgaagcagt?tattacttgc?1200

agaccagccg?acctaatcga?gcctcaaatg?atttatctac?gtgacgagat?tgctccatac?1260

gctcattcag?aagaagacgt?gttaagctat?gcaagcttcc?cgcaacaagc?tagagatttc?1320

ttgggacgcc?gtgaagatcc?tttctatgat?gttccggttc?aagaagttac?tgtacaactg?1380

gacattcaag?actaa 1395

<210>28

<211>464

<212>PRT

＜213〉streptococcus pyogenes

<400>28

Met?Thr?Lys?Ile?Arg?Ile?Thr?Glu?Thr?Val?Leu?Arg?Asp?Gly?Gln?Gln

1 5 10 15

Ser?Gln?Ile?Ala?Thr?Arg?Met?Thr?Thr?Lys?Glu?Met?Ile?Pro?Ile?Leu

20 25 30

Glu?Thr?Leu?Asp?Asn?Ala?Gly?Tyr?His?Ala?Leu?Glu?Met?Trp?Gly?Gly

35 40 45

Ala?Thr?Phe?Asp?Ser?Cys?Leu?Arg?Phe?Leu?Asn?Glu?Asp?Pro?Trp?Glu

50 55 60

Arg?Leu?Arg?Ala?Ile?Arg?Lys?Ala?Val?Lys?Lys?Thr?Lys?Leu?Gln?Met

65 70 75 80

Leu?Leu?Arg?Gly?Gln?Asn?Leu?Leu?Gly?Tyr?Arg?Asn?Tyr?Ala?Asp?Asp

85 90 95

Val?Val?Arg?Ser?Phe?Ile?Gln?Lys?Ser?Ile?Glu?Asn?Gly?Ile?Asp?Ile

100 105 110

Val?Arg?Ile?Phe?Asp?Ala?Leu?Asn?Asp?Pro?Arg?Asn?Leu?Gln?Thr?Ala

115 120 125

Val?Ser?Ala?Thr?Lys?Lys?Phe?Gly?Gly?His?Ala?Gln?Val?Ala?Ile?Ser

130 135 140

Tyr?Thr?Thr?Ser?Pro?Val?His?Thr?Ile?Asp?Tyr?Phe?Val?Glu?Leu?Ala

145 150 155 160

Lys?Ala?Tyr?Gln?Ala?Ile?Gly?Ala?Asp?Ser?Ile?Cys?Ile?Lys?Asp?Met

165 170 175

Ala?Gly?Val?Leu?Thr?Pro?Glu?Ile?Gly?Tyr?Gln?Leu?Val?Lys?Cys?Ile

180 185 190

Lys?Glu?Asn?Thr?Thr?Ile?Pro?Leu?Glu?Val?His?Thr?His?Ala?Thr?Ser

195 200 205

Gly?Ile?Ser?Glu?Met?Thr?Tyr?Leu?Lys?ValAla?Glu?Ala?Gly?Ala?Asp

210 215 220

Ile?Ile?Asp?Thr?Ala?Ile?Ser?Ser?Phe?Ser?Gly?Gly?Thr?Ser?Gln?Pro

225 230 235 240

Ala?Thr?Glu?Ser?Met?Ala?Ile?Ala?Leu?Thr?Asp?Leu?Gly?Phe?Asp?Thr

245 250 255

Gly?Leu?Asp?Met?Gln?Glu?Val?Ala?Lys?Val?Ala?Glu?Tyr?Phe?Asn?Thr

260 265 270

Ile?Arg?Asp?His?Tyr?Arg?Glu?Ile?Gly?Ile?Leu?Asn?Pro?Lys?Val?Lys

275 280 285

Asp?Thr?Glu?Pro?Lys?Thr?Leu?Ile?Tyr?Gln?Val?Pro?Gly?Gly?Met?Leu

290 295 300

Ser?Asn?Leu?Leu?Ser?Gln?Leu?Thr?Glu?Gln?Gly?Leu?Thr?Asp?Lys?Tyr

305 310 315 320

Glu?Glu?Val?Leu?Ala?Glu?Val?Pro?Lys?Val?Arg?Ala?Asp?Leu?Gly?Tyr

325 330 335

Pro?Pro?Leu?Val?Thr?Pro?Leu?Ser?Gln?Met?Val?Gly?Thr?Gln?Ala?Leu

340 345 350

Met?Asn?Ile?Ile?Ser?Gly?Glu?Arg?Tyr?Lys?Val?Val?Pro?Asn?Glu?Ile

355 360 365

Lys?Asp?Tyr?Val?Arg?Gly?Leu?Tyr?Gly?Gln?Ser?Pro?Ala?Pro?Leu?Ala

370 375 380

Glu?GIy?Ile?Lys?Glu?Lys?Ile?Ile?Gly?Asp?Glu?Ala?Val?Ile?Thr?Cys

385 390 395 400

Arg?Pro?Ala?Asp?Leu?Ile?Glu?Pro?Gln?Met?Ile?Tyr?Leu?Arg?Asp?Glu

405 410 415

Ile?Ala?Pro?Tyr?Ala?His?Ser?Glu?Glu?Asp?Val?Leu?Ser?Tyr?Ala?Ser

420 425 430

Phe?Pro?Gln?Gln?Ala?Arg?Asp?Phe?Leu?Gly?Arg?Arg?Glu?Asp?Pro?Phe

435 440 445

Tyr?Asp?Val?Pro?Val?Gln?Glu?Val?Thr?Val?Gln?Leu?Asp?Ile?Gln?Asp

450 455 460

<210>29

<211>1224

<212>DNA

＜213〉streptococcus pyogenes

<400>29

atgacgaatt?taacgtttta?tgcaaaaatc?ggtatttcag?aagaagagca?tgactctttt?60

gttaaagaac?atcagcaaat?tagcgtttta?caaggtagtg?attgggcaaa?aatcaaaaat?120

caatggcaga?atgagcgaat?tggtatctat?aaagaggaaa?agcaggttgc?ctctttatca?180

cttttgatta?agctattgcc?acttggaaga?agcattattt?atattccaag?agggccagtc?240

atggattatc?ttgaccgtga?tttggtggca?tttaccatga?aaacactaaa?ggattatggt?300

aaaactaaaa?aggccctctt?tatcaaatat?gatccagcta?tcctgttaaa?acaatacgca?360

ctgggacagg?aagaagaaga?aaaaccttta?gctttagcag?ctattaagaa?tctccaagaa?420

gctggtgttc?attggactgg?tttaacaatg?gagattgcag?atagtatcca?acctcgtttc?480

caagctaata?tttacactca?agaaaacctt?gagatgcaat?ttcctaagca?taccagacgt?540

ttaataaaag?atgctaagca?gcgtggtgta?aaaacatatc?gtgtcagtca?atcagaactt?600

cacaaatttt?ccaagattgt?ctccttaaca?gaaaaacgta?aaaatatttc?tttgcgtaac?660

gaagcttact?ttcaaaagtt?gatgactact?tatggggata?aggcctactt?acatctagca?720

aaagtgaata?ttcctcaaaa?actagatcaa?taccgccagc?aattaattct?tattaaccaa?780

gatattactc?gcacccaagc?tcatcaaaag?aagcgtttaa?aaaaattaga?agatcaaaaa?840

gcttctttag?aacgttatat?aactgaattt?gaaggcttta?cagaccaata?tcctgaggaa?900

gttgttgtag?caggtatatt?atctatttct?tatggaaatg?ttatggaaat?gctttatgct?960

gggatgaatg?atgattttaa?gaagttttat?cctcagtatc?tgctgtatcc?taatgttttt?1020

caggatgctt?atcaagatgg?tattatttgg?gctaacatgg?gaggagtaga?aggctcgctt?1080

gatgatggac?ttaccaaatt?taaggccaac?tttgctccga?caatagaaga?atttatagga?1140

gaatttaatc?tccctgtcag?cccactttat?catattgcta?ataccatgta?caaaatacga?1200

aaacagttaa?agaataaaca?ttaa 1224

<210>30

<211>407

<212>PRT

＜213〉streptococcus pyogenes

<400>30

Met?Thr?Asn?Leu?Thr?Phe?Tyr?Ala?Lys?Ile?Gly?Ile?Ser?Glu?Glu?Glu

1 5 10 15

His?Asp?Ser?Phe?Val?Lys?Glu?His?Gln?Gln?Ile?Ser?Val?Leu?Gln?Gly

20 25 30

Ser?Asp?Trp?Ala?Lys?Ile?Lys?Asn?Gln?Trp?Gln?Asn?Glu?Arg?Ile?Gly

35 40 45

Ile?Tyr?Lys?Glu?Glu?Lys?Gln?Val?Ala?Ser?Leu?Ser?Leu?Leu?Ile?Lys

50 55 60

Leu?Leu?Pro?Leu?Gly?Arg?Ser?Ile?Ile?Tyr?Ile?Pro?Arg?Gly?Pro?Val

65 70 75 80

Met?Asp?Tyr?Leu?Asp?Arg?Asp?Leu?Val?Ala?Phe?Thr?Met?Lys?Thr?Leu

85 90 95

Lys?Asp?Tyr?Gly?Lys?Thr?Lys?Lys?Ala?Leu?Phe?Ile?Lys?Tyr?Asp?Pro

100 105 110

Ala?Ile?Leu?Leu?Lys?Gln?Tyr?Ala?Leu?Gly?Gln?Glu?Glu?Glu?Glu?Lys

115 120 125

Pro?Leu?Ala?Leu?Ala?Ala?Ile?Lys?Asn?Leu?Gln?Glu?Ala?Gly?Val?His

130 135 140

Trp?Thr?Gly?Leu?Thr?Met?Glu?Ile?Ala?Asp?Ser?Ile?Gln?Pro?Arg?Phe

145 150 155 160

Gln?Ala?Asn?Ile?Tyr?Thr?Gln?Glu?Asn?Leu?Glu?Met?Gln?Phe?Pro?Lys

165 170 175

His?Thr?Arg?Arg?Leu?Ile?Lys?Asp?Ala?Lys?Gln?Arg?Gly?Val?Lys?Thr

180 185 190

Tyr?Arg?Val?Ser?Gln?Ser?Glu?Leu?His?Lys?Phe?Ser?Lys?Ile?Val?Ser

195 200 205

Leu?Thr?Glu?Lys?Arg?Lys?Asn?Ile?Ser?Leu?Arg?Asn?Glu?Ala?Tyr?Phe

210 215 220

Gln?Lys?Leu?Met?Thr?Thr?Tyr?Gly?Asp?Lys?Ala?Tyr?Leu?His?Leu?Ala

225 230 235 240

Lys?Val?Asn?Ile?Pro?Gln?Lys?Leu?Asp?Gln?Tyr?Arg?Gln?Gln?Leu?Ile

245 250 255

Leu?Ile?Asn?Gln?Asp?Ile?Thr?Arg?Thr?Gln?Ala?His?Gln?Lys?Lys?Arg

260 265 270

Leu?Lys?Lys?Leu?Glu?Asp?Gln?Lys?Ala?Ser?Leu?Glu?Arg?Tyr?Ile?Thr

275 280 285

Glu?Phe?Glu?Gly?Phe?Thr?Asp?Gln?Tyr?Pro?Glu?Glu?Val?Val?Val?Ala

290 295 300

Gly?Ile?Leu?Ser?Ile?Ser?Tyr?Gly?Asn?Val?Met?Glu?Met?Leu?Tyr?Ala

305 310 315 320

Gly?Met?Asn?Asp?Asp?Phe?Lys?Lys?Phe?Tyr?Pro?Gln?Tyr?Leu?Leu?Tyr

325 330 335

Pro?Asn?Val?Phe?Gln?Asp?Ala?Tyr?Gln?Asp?Gly?Ile?Ile?Trp?Ala?Asn

340 345 350

Met?Gly?Gly?Val?Glu?Gly?Ser?Leu?Asp?Asp?Gly?Leu?Thr?Lys?Phe?Lys

355 360 365

Ala?Asn?Phe?Ala?Pro?Thr?Ile?Glu?Glu?Phe?Ile?Gly?Glu?Phe?Asn?Leu

370 375 380

Pro?Val?Ser?Pro?Leu?Tyr?His?Ile?Ala?Asn?Thr?Met?Tyr?Lys?Ile?Arg

385 390 395 400

Lys?Gln?Leu?Lys?Asn?Lys?His

405

<210>31

<211>483

<212>DNA

＜213〉streptococcus pyogenes

<400>31

atgccaaaga?gaataagaaa?agctctagga?gtagttggga?aactgatgtc?aattgttcct?60

gacactactg?aaattattgg?aaaaacgatt?gataacagtc?gaccaattat?cgaaaaacgt?120

atggaacaaa?aacatgaaaa?agagatgcaa?ttacgcacga?taaatgatgt?gattaatctt?180

cctgttgatc?aggcacaagc?tcatttggaa?caacttggct?ttgtagtagc?cactatacct?240

gctaagcctc?ataaaaaatg?gcttcatagt?aatctcaatg?aagtggttgc?tatgtctcca?300

aaatctggta?aatataagat?aggaagcctg?atcaaactct?actacattac?tgttgatgtt?360

ttggaaaaaa?gtcaagactt?attagaccaa?gaaaacttgc?ggactgtgga?acgcaatcaa?420

aaaatcgctg?atacttttga?gtctgtcaaa?cacattcgat?ttccttttaa?aaaatggcgt?480

taa 483

<210>32

<211>160

<212>PRT

＜213〉streptococcus pyogenes

<400>32

Met?Pro?Lys?Arg?Ile?Arg?Lys?Ala?Leu?Gly?Val?Val?Gly?Lys?Leu?Met

1 5 10 15

Ser?Ile?Val?Pro?Asp?Thr?Thr?Glu?Ile?Ile?Gly?Lys?Thr?Ile?Asp?Asn

20 25 30

Ser?Arg?Pro?Ile?Ile?Glu?Lys?Arg?Met?Glu?Gln?Lys?His?Glu?Lys?Glu

35 40 45

Met?Gln?Leu?Arg?Thr?Ile?Asn?Asp?Val?Ile?Asn?Leu?Pro?Val?Asp?Gln

50 55 60

Ala?Gln?Ala?His?Leu?Glu?Gln?Leu?Gly?Phe?Val?Val?Ala?Thr?Ile?Pro

65 70 75 80

Ala?Lys?Pro?His?Lys?Lys?Trp?Leu?His?Ser?Asn?Leu?Asn?Glu?Val?Val

85 90 95

Ala?Met?Ser?Pro?Lys?Ser?Gly?Lys?Tyr?Lys?Ile?Gly?Ser?Leu?Ile?Lys

100 105 110

Leu?Tyr?Tyr?Ile?Thr?Val?Asp?Val?Leu?Glu?Lys?Ser?Gln?Asp?Leu?Leu

115 120 125

Asp?Gln?Glu?Asn?Leu?Arg?Thr?Val?Glu?Arg?Asn?Gln?Lys?Ile?Ala?Asp

130 135 140

Thr?Phe?Glu?Ser?Val?Lys?His?Ile?Arg?Phe?Pro?Phe?Lys?Lys?Trp?Arg

145 150 155 160

<210>33

<211>1345

<212>DNA

＜213〉streptococcus pyogenes

<400>33

atgatgttaa?cgaaggggca?acttccctat?agtgctgctt?ttacaacagg?agggctcttt?60

tactttgtta?tcattgcatt?aagttattat?ttgggatcga?cactttggct?agtctttgtt?120

caggtgtttt?gtttttactt?atctggttta?tatctttata?aactcattaa?ttatatgaca?180

ggctttcaaa?aagtggcttt?aactttttca?attagctact?atttattatc?tgttagtctt?240

ggttttgggg?gattgtatcc?tactcaactg?gctatgccat?ttatattaat?atcggcttgg?300

tttttaacta?agtattttgc?ctgtttagtg?aaagatgagg?catttattct?ttttggcttt?360

gtaggtgctc?ttgcaatgct?aattgacccg?agtaccctta?tcttttggtc?ttttgcttgt?420

gtgacagttt?tttcttataa?tataagccaa?aagcatcttg?caagaggttt?ttatcaactg?480

ctagcttcga?tttttggaat?gattttagtt?ttttacacag?caggatattt?cattttgaac?540

ttacaagtgc?taaatcctta?tttatcacaa?acgatgattt?atccttttac?tttttttaaa?600

tcaggaaact?tatcgttgct?ttttggactg?gctattcagt?tgttcttcgc?tttggggctt?660

ggtcttttga?cgggaatgga?gaatgtcatt?aggcgattta?aaaacaattc?tgatagggtc?720

gtcaagtggc?tatttgtcat?ggtcattcta?gaatctatac?ttgtggctat?attttcacaa?780

gactatcgcc?cctatcatct?tttacctctt?ttaccttttg?gattaatttt?gactgctatt?840

cctgttggct?atcagtatgg?tataggatta?ggtcagagta?gtcatcgcag?acgtcatggt?900

aaaaatggtg?ttggtcgagt?aatgatgatt?tatcttaaga?gacactttta?tttgccaatt?960

ttaattgtag?ggacaatact?aatctgttct?acttattgtt?tcattagtag?tattcctctt?1020

aatcaggagc?gtgatcatat?tgctagttat?ttagaacaga?aactaaataa?aactcaatct?1080

atttatgttt?gggatgatac?ttctaaaatt?tatttggaca?gtaaagctaa?atctgtttct?1140

caatttagtt?ctcctgacat?caatacgcaa?aaagagagtc?atcgaaaaat?attagaagat?1200

gaactattag?aaaataaggc?tgcttatatc?gttgttaatc?gctataaaaa?cctgcctaaa?1260

atcattcaaa?aagtattatc?tactaattac?aaagtagata?aacagataac?gacaaaaagt?1320

tttattgttt?atcagaaaaa?gtaat 1345

<210>34

<211>447

<212>PRT

＜213〉streptococcus pyogenes

<400>34

Met?Met?Leu?Thr?Lys?Gly?Gln?Leu?Pro?Tyr?Ser?Ala?Ala?Phe?Thr?Thr

1 5 10 15

Gly?Gly?Leu?Phe?Tyr?Phe?Val?Ile?Ile?Ala?Leu?Ser?Tyr?Tyr?Leu?Gly

20 25 30

Ser?Thr?Leu?Trp?Leu?Val?Phe?Val?Gln?Val?Phe?Cys?Phe?Tyr?Leu?Ser

35 40 45

Gly?Leu?Tyr?Leu?Tyr?Lys?Leu?Ile?Asn?Tyr?Met?Thr?Gly?Phe?Gln?Lys

50 55 60

Val?Ala?Leu?Thr?Phe?Ser?Ile?Ser?Tyr?Tyr?Leu?Leu?Ser?Val?Ser?Leu

65 70 75 80

Gly?Phe?Gly?Gly?Leu?Tyr?Pro?Thr?Gln?Leu?Ala?Met?Pro?Phe?Ile?Leu

85 90 95

Ile?Ser?Ala?Trp?Phe?Leu?Thr?Lys?Tyr?Phe?Ala?Cys?Leu?Val?Lys?Asp

100 105 110

Glu?Ala?Phe?Ile?Leu?Phe?Gly?Phe?Val?Gly?Ala?Leu?Ala?Met?Leu?Ile

115 120 125

Asp?Pro?Ser?Thr?Leu?Ile?Phe?Trp?Ser?Phe?Ala?Cys?Val?Thr?Val?Phe

130 135 140

Ser?Tyr?Asn?Ile?Ser?Gln?Lys?His?Leu?Ala?Arg?Gly?Phe?Tyr?Gln?Leu

145 150 155 160

Leu?Ala?Ser?Ile?Phe?Gly?Met?Ile?Leu?Val?Phe?Tyr?Thr?Ala?Gly?Tyr

165 170 175

Phe?Ile?Leu?Asn?Leu?Gln?Val?Leu?Asn?Pro?Tyr?Leu?Ser?Gln?Thr?Met

180 185 190

Ile?Tyr?Pro?Phe?Thr?Phe?Phe?Lys?Ser?Gly?Asn?Leu?Ser?Leu?Leu?Phe

195 200 205

Gly?Leu?Ala?Ile?Gln?Leu?Phe?Phe?Ala?Leu?Gly?Leu?Gly?Leu?Leu?Thr

210 215 220

Gly?Met?Glu?Asn?Val?Ile?Arg?Arg?Phe?Lys?Asn?Asn?Ser?Asp?Arg?Val

225 230 235 240

Val?Lys?Trp?Leu?Phe?Val?Met?Val?Ile?Leu?Glu?Ser?Ile?Leu?Val?Ala

245 250 255

Ile?Phe?Ser?Gln?Asp?Tyr?Arg?Pro?Tyr?His?Leu?Leu?Pro?Leu?Leu?Pro

260 265 270

Phe?Gly?Leu?Ile?Leu?Thr?Ala?Ile?Pro?Val?Gly?Tyr?Gln?Tyr?Gly?Ile

275 280 285

Gly?Leu?Gly?Gln?Ser?Ser?His?Arg?Arg?Arg?His?Gly?Lys?Asn?Gly?Val

290 295 300

Gly?Arg?Val?Met?Met?Ile?Tyr?Leu?Lys?Arg?His?Phe?Tyr?Leu?Pro?Ile

305 310 315 320

Leu?Ile?Val?Gly?Thr?Ile?Leu?Ile?Cys?Ser?Thr?Tyr?Cys?Phe?Ile?Ser

325 330 335

Ser?Ile?Pro?Leu?Asn?Gln?Glu?Arg?Asp?His?Ile?Ala?Ser?Tyr?Leu?Glu

340 345 350

Gln?Lys?Leu?Asn?Lys?Thr?Gln?Ser?Ile?Tyr?Val?Trp?Asp?Asp?Thr?Ser

355 360 365

Lys?Ile?Tyr?Leu?Asp?Ser?Lys?Ala?Lys?Ser?Val?Ser?Gln?Phe?Ser?Ser

370 375 380

Pro?Asp?Ile?Asn?Thr?Gln?Lys?Glu?Ser?His?Arg?Lys?Ile?Leu?Glu?Asp

385 390 395 400

Glu?Leu?Leu?Glu?Asn?Lys?Ala?Ala?Tyr?Ile?Val?Val?Asn?Arg?Tyr?Lys

405 410 415

Asn?Leu?Pro?Lys?Ile?Ile?Gln?Lys?Val?Leu?Ser?Thr?Asn?Tyr?Lys?Val

420 425 430

Asp?Lys?Gln?Ile?Thr?Thr?Lys?Ser?Phe?Ile?Val?Tyr?Gln?Lys?Lys

435 440 445

<210>35

<211>618

<212>DNA

＜213〉streptococcus pyogenes

<400>35

atgattaaag?ctcagctaaa?gaatctaata?aaaacaattc?atctcgtaaa?agaggctggt?60

ttctccctaa?atggttcatt?gtacatggag?ttattgcatg?attttacgca?tgatgagtta?120

gtacataacc?ctataatcga?aagtacggtt?gatacaattc?agtcagttga?atttgaaagg?180

gtttcttttt?catataatgg?taaggataat?gtaataaacg?atataagttt?ttcaattaaa?240

gcaggagaga?ggttagcaat?tgttggtgga?aatggttctg?gaaaaagtac?gattttcaaa?300

ttagcctgcg?gtctttacga?taattatgaa?ggcaatatat?atataaatgg?tattaatctt?360

tgttcaatac?agaaaaagtc?atactacaag?agaatatccg?cattgttcca?agatttttta?420

aagtatgaat?tgacactgcg?tgaaaatgtt?ggattgggtg?aattatcaaa?gttatatagt?480

gatgagtctt?ataatacttc?attttttacc?acatcatggg?attcagagaa?tgaactgcga?540

ttggaggata?tttatataag?atatagtgat?ggtaagtttc?attttacaac?tatcgatgaa?600

tcgattaaac?gggattaa 618

<210>36

<211>205

<212>PRT

＜213〉streptococcus pyogenes

<400>36

Met?Ile?Lys?Ala?Gln?Leu?Lys?Asn?Leu?Ile?Lys?Thr?Ile?His?Leu?Val

1 5 10 15

Lys?Glu?Ala?Gly?Phe?Ser?Leu?Asn?Gly?Ser?Leu?Tyr?Met?Glu?Leu?Leu

20 25 30

His?Asp?Phe?Thr?His?Asp?Glu?Leu?Val?His?Asn?Pro?Ile?Ile?Glu?Ser

35 40 45

Thr?Val?Asp?Thr?Ile?Gln?Ser?Val?Glu?Phe?Glu?Arg?Val?Ser?Phe?Ser

50 55 60

Tyr?Asn?Gly?Lys?Asp?Asn?Val?Ile?Asn?Asp?Ile?Ser?Phe?Ser?Ile?Lys

65 70 75 80

Ala?Gly?Glu?Arg?Leu?Ala?Ile?Val?Gly?Gly?Asn?Gly?Ser?Gly?Lys?Ser

85 90 95

Thr?Ile?Phe?Lys?Leu?Ala?Cys?Gly?Leu?Tyr?Asp?Asn?Tyr?Glu?Gly?Asn

100 105 110

Ile?Tyr?Ile?Asn?Gly?Ile?Asn?Leu?Cys?Ser?Ile?Gln?Lys?Lys?Ser?Tyr

115 120 125

Tyr?Lys?Arg?Ile?Ser?Ala?Leu?Phe?Gln?Asp?Phe?Leu?Lys?Tyr?Glu?Leu

130 135 140

Thr?Leu?Arg?Glu?Asn?Val?Gly?Leu?Gly?Glu?Leu?Ser?Lys?Leu?Tyr?Ser

145 150 155 160

Asp?Glu?Ser?Tyr?Asn?Thr?Ser?Phe?Phe?Thr?Thr?Ser?Trp?Asp?Ser?Glu

165 170 175

Asn?Glu?Leu?Arg?Leu?Glu?Asp?Ile?Tyr?Ile?Arg?Tyr?Ser?Asp?Gly?Lys

180 185 190

Phe?His?Phe?Thr?Thr?Ile?Asp?Glu?Ser?Ile?Lys?Arg?Asp

195 200 205

<210>37

<211>1434

<212>DNA

＜213〉streptococcus pyogenes

<400>37

atgctaagaa?aaaagagaat?tttgggttta?tttcgacttg?ttgaattaat?tttcctagga?60

ttattgttga?gtttagttgt?ttcgtacctt?gcgtggacca?atagttttgc?aacacttcat?120

aatatattag?caactgtcgg?aatagtggaa?cgaagcaaag?atcaacagcc?tcgctatcac?180

attggacaag?ctattcaagt?acaaaaaagt?ggtccatatc?atcaatggat?tggaactatt?240

aataaacagg?tagaagatat?agccgaaaat?taccgagtga?gttatcatta?tgaagtggta?300

tttccaatag?gaaaagtcac?tgtttctttg?ccagaacata?acctgaaaga?gcctgataaa?360

ccgaggttta?aaaaaggaga?tatcgttaaa?ttatcttcat?taactaaaaa?gccacatata?420

aaagtatatc?aaggtcaatt?agcgactatt?aaacaagtta?aaaaatgcta?tgactattcg?480

ttaggaggat?atcagtacga?tattaatctg?aaagataatc?taagattaga?tggaatttca?540

gagcaagatt?ttgttaaacc?ttattatatt?aggttcaata?aaggaaattc?ccctgagcaa?600

aacaatcgtc?ttttgcgaaa?agctttcgct?tatgcaaagc?agcatccaaa?tagcgttata?660

tcttttccaa?aggggcaatt?tcacattggc?tctttgcctt?cacaaaaaga?ttattttgag?720

cttccatctg?atacagctat?tattggtcat?cagacagagt?tcattattca?cggtaaaatg?780

ttgtggtttg?gattccctac?aggaccaaag?gctgaacaag?gtgttcgtaa?tctcgtgttg?840

actggagtgc?atttcaaagc?aaatgatttg?aaaaaaggag?accactttat?gattatggct?900

gatcatggta?ctgattggca?tatttacgat?aacaaattta?ctatggttca?taagcgtaat?960

agtcatattt?ttgatttagg?atctctacaa?aattcattgt?ttgagaaaaa?ccaatttatt?1020

ggctatgcgc?cagaattagt?acaagaccaa?cagctgctat?caaaggctca?agggcatgat?1080

tttttttcag?aagtcattca?gtttgatgct?gctgttcatc?attttgcatg?ggatggaggt?1140

ctacttagta?atattgctcc?aaactatgaa?gcatttaacc?aaactcgaca?tctatgtcac?1200

aatattactg?taagccaaaa?tcaattttta?ccttatatag?atccgactgg?ttgcctgaga?1260

gcctatagtg?gttctattgg?tcagcattcc?tcaaaagtag?gagttattag?ggttttaaat?1320

aatgttttta?cctcatccat?tgttactaaa?gcgaagctca?ctagttggtt?tatggaacct?1380

attcattttc?caccaaattc?accggttatt?gtcgcaggta?atatcattaa?ttga 1434

<210>38

<211>477

<212>PRT

＜213〉streptococcus pyogenes

<400>38

Met?Leu?Arg?Lys?Lys?Arg?Ile?Leu?Gly?Leu?Phe?Arg?Leu?Val?Glu?Leu

1 5 10 15

Ile?Phe?Leu?Gly?Leu?Leu?Leu?Ser?Leu?Val?Val?Ser?Tyr?Leu?Ala?Trp

20 25 30

Thr?Asn?Ser?Phe?Ala?Thr?Leu?His?Asn?Ile?Leu?Ala?Thr?Val?Gly?Ile

35 40 45

Val?Glu?Arg?Ser?Lys?Asp?Gln?Gln?Pro?Arg?Tyr?His?Ile?Gly?Gln?Ala

50 55 60

Ile?Gln?Val?Gln?Lys?Ser?Gly?Pro?Tyr?His?Gln?Trp?Ile?Gly?Thr?Ile

65 70 75 80

Asn?Lys?Gln?Val?Glu?Asp?Ile?Ala?Glu?Asn?Tyr?Arg?Val?Ser?Tyr?His

85 90 95

Tyr?Glu?Val?Val?Phe?Pro?Ile?Gly?Lys?Val?Thr?Val?Ser?Leu?Pro?Glu

100 105 110

His?Asn?Leu?Lys?Glu?Pro?Asp?Lys?Pro?Arg?Phe?Lys?Lys?Gly?Asp?Ile

115 120 125

Val?Lys?Leu?Ser?Ser?Leu?Thr?Lys?Lys?Pro?His?Ile?Lys?Val?Tyr?Gln

130 135 140

Gly?Gln?Leu?Ala?Thr?Ile?Lys?Gln?Val?Lys?Lys?Cys?Tyr?Asp?Tyr?Ser

145 150 155 160

Leu?Gly?Gly?Tyr?Gln?Tyr?Asp?Ile?Asn?Leu?Lys?Asp?Asn?Leu?Arg?Leu

165 170 175

Asp?Gly?Ile?Ser?Glu?Gln?Asp?Phe?Val?Lys?Pro?Tyr?Tyr?Ile?Arg?Phe

180 185 190

Asn?Lys?Gly?Asn?Ser?Pro?Glu?Gln?Asn?Asn?Arg?Leu?Leu?Arg?Lys?Ala

195 200 205

Phe?Ala?Tyr?Ala?Lys?Gln?His?Pro?Asn?Ser?Val?Ile?Ser?Phe?Pro?Lys

210 215 220

Gly?Gln?Phe?His?Ile?Gly?Ser?Leu?Pro?Ser?Gln?Lys?Asp?Tyr?Phe?Glu

225 230 235 240

Leu?Pro?Ser?Asp?Thr?Ala?Ile?Ile?Gly?His?Gln?Thr?Glu?Phe?Ile?Ile

245 250 255

His?Gly?Lys?Met?Leu?Trp?Phe?Gly?Phe?Pro?Thr?Gly?Pro?Lys?Ala?Glu

260 265 270

Gln?Gly?Val?Arg?Asn?Leu?Val?Leu?Thr?Gly?Val?His?Phe?Lys?Ala?Asn

275 280 285

Asp?Leu?Lys?Lys?Gly?Asp?His?Phe?Met?Ile?Met?Ala?Asp?His?Gly?Thr

290 295 300

Asp?Trp?His?Ile?Tyr?Asp?Asn?Lys?Phe?Thr?Met?Val?His?Lys?Arg?Asn

305 310 315 320

Ser?His?Ile?Phe?Asp?Leu?Gly?Ser?Leu?Gln?Asn?Ser?Leu?Phe?Glu?Lys

325 330 335

Asn?Gln?Phe?Ile?Gly?Tyr?Ala?Pro?Glu?Leu?Val?Gln?Asp?Gln?Gln?Leu

340 345 350

Leu?Ser?Lys?Ala?Gln?Gly?His?Asp?Phe?Phe?Ser?Glu?Val?Ile?Gln?Phe

355 360 365

Asp?Ala?Ala?Val?His?His?Phe?Ala?Trp?Asp?Gly?Gly?Leu?Leu?Ser?Asn

370 375 380

Ile?Ala?Pro?Asn?Tyr?Glu?Ala?Phe?Asn?Gln?Thr?Arg?His?Leu?Cys?His

385 390 395 400

Asn?Ile?Thr?Val?Ser?Gln?Asn?Gln?Phe?Leu?Pro?Tyr?Ile?Asp?Pro?Thr

405 410 415

Gly?Cys?Leu?Arg?Ala?Tyr?Ser?Gly?Ser?Ile?Gly?Gln?His?Ser?Ser?Lys

420 425 430

Val?Gly?Val?Ile?Arg?Val?Leu?Asn?Asn?Val?Phe?Thr?Ser?Ser?Ile?Val

435 440 445

Thr?Lys?Ala?Lys?Leu?Thr?Ser?Trp?Phe?Met?Glu?Pro?Ile?His?Phe?Pro

450 455 460

Pro?Asn?Ser?Pro?Val?Ile?Val?Ala?Gly?Asn?Ile?Ile?Asn

465 470 475

<210>39

<211>1020

<212>DNA

＜213〉streptococcus pyogenes

<400>39

atgtcaactc?acttaagaaa?acttccaggg?ctgttacttt?gcttattgtt?agctcttcca?60

gcctggtgtt?tagggcgctt?atttcccatt?attggagcac?ctgtttttgc?tattctttta?120

ggaatgttgt?tagccttgtt?ttatgaacat?cgtgacaaga?ctaaagaggg?aattagtttt?180

acatccaagt?atattttaca?aacagcagtg?gttttgcttg?gttttggatt?aaacctaacc?240

caagttatgg?cagtgggcat?gcagtcttta?ccgattatca?tttcaactat?tgcgacagct?300

cttttggtag?cttatggctt?acagaaatgg?ctgcgcttag?atgtcaatac?agccaccttg?360

gtaggtgtag?gatcttccat?ttgtgggggg?tctgctgttg?cagcgacagc?tcctgtcatt?420

aaggcaaagg?atgacgaggt?tgctaaggca?atttcagtca?tttttctctt?taatatgtta?480

gcagctttgc?tatttccaag?tttaggacaa?ttactaggct?tatctaatga?aggttttgct?540

atttttgccg?ggacagctgt?taacgacact?tcttccgtga?ctgcaacggc?cacggcctgg?600

gatgcccttc?accattccaa?tacactagat?ggagcaacca?ttgttaaatt?gactcgcacc?660

ttggctattc?tcccaattac?tttaggttta?tccctttacc?gagcgaaaaa?agagcacgac?720

atcgttacag?aagaaaactt?tagccttagg?aagtcattcc?ctcgtttcat?cctcttcttt?780

ttattagctt?ctctcatcac?aacattgatg?accagtttgg?gagtttctgc?cgatagtttc?840

cattacctaa?aaaccttatc?aaaattcttt?atcgtgatgg?ctatggcagc?gattggttta?900

aacacaaacc?tggttaaact?gattaagacg?ggcggtcagg?ctatcctttt?aggagctatt?960

tgctgggtag?ctatcaccct?tgtcagttta?gccatgcaat?taagtttggg?catttggtaa?1020

<210>40

<211>339

<212>PRT

＜213〉streptococcus pyogenes

<400>40

Met?Ser?Thr?His?Leu?Arg?Lys?Leu?Pro?Gly?Leu?Leu?Leu?Cys?Leu?Leu

1 5 10 15

Leu?Ala?Leu?Pro?Ala?Trp?Cys?Leu?Gly?Arg?Leu?Phe?Pro?Ile?Ile?Gly

20 25 30

Ala?Pro?Val?Phe?Ala?Ile?Leu?Leu?Gly?Met?Leu?Leu?Ala?Leu?Phe?Tyr

35 40 45

Glu?His?Arg?Asp?Lys?Thr?Lys?Glu?Gly?Ile?Ser?Phe?Thr?Ser?Lys?Tyr

50 55 60

Ile?Leu?Gln?Thr?Ala?Val?Val?Leu?Leu?Gly?Phe?Gly?Leu?Asn?Leu?Thr

65 70 75 80

Gln?Val?Met?Ala?Val?Gly?Met?Gln?Ser?Leu?Pro?Ile?Ile?Ile?Ser?Thr

85 90 95

Ile?Ala?Thr?Ala?Leu?Leu?Val?Ala?Tyr?Gly?Leu?Gln?Lys?Trp?Leu?Arg

100 105 110

Leu?Asp?Val?Asn?Thr?Ala?Thr?Leu?Val?Gly?Val?Gly?Ser?Ser?Ile?Cys

115 120 125

Gly?Gly?Ser?Ala?Val?Ala?Ala?Thr?Ala?Pro?Val?Ile?Lys?Ala?Lys?Asp

130 135 140

Asp?Glu?Val?Ala?Lys?Ala?Ile?Ser?Val?Ile?Phe?Leu?Phe?Asn?Met?Leu

145 150 155 160

Ala?Ala?Leu?Leu?Phe?Pro?Ser?Leu?Gly?Gln?Leu?Leu?Gly?Leu?Ser?Asn

165 170 175

Glu?Gly?Phe?Ala?Ile?Phe?Ala?Gly?Thr?Ala?Val?Asn?Asp?Thr?Ser?Ser

180 185 190

Val?Thr?Ala?Thr?Ala?Thr?Ala?Trp?Asp?Ala?Leu?His?His?Ser?Asn?Thr

195 200 205

Leu?Asp?Gly?Ala?Thr?Ile?Val?Lys?Leu?Thr?Arg?Thr?Leu?Ala?Ile?Leu

210 215 220

Pro?Ile?Thr?Leu?Gly?Leu?Ser?Leu?Tyr?Arg?Ala?Lys?Lys?Glu?His?Asp

225 230 235 240

Ile?Val?Thr?Glu?Glu?Asn?Phe?Ser?Leu?Arg?Lys?Ser?Phe?Pro?Arg?Phe

245 250 255

Ile?Leu?Phe?Phe?Leu?Leu?Ala?Ser?Leu?Ile?Thr?Thr?Leu?Met?Thr?Ser

260 265 270

Leu?Gly?Val?Ser?Ala?Asp?Ser?Phe?His?Tyr?Leu?Lys?Thr?Leu?Ser?Lys

275 280 285

Phe?Phe?Ile?Val?Met?Ala?Met?Ala?Ala?Ile?Gly?Leu?Asn?Thr?Asn?Leu

290 295 300

Val?Lys?Leu?Ile?Lys?Thr?Gly?Gly?Gln?Ala?Ile?Leu?Leu?Gly?Ala?Ile

305 310 315 320

Cys?Trp?Val?Ala?Ile?Thr?Leu?Val?Ser?Leu?Ala?Met?Gln?Leu?Ser?Leu

325 330 335

Gly?Ile?Trp

<210>41

<211>2637

<212>DNA

＜213〉streptococcus pyogenes

<400>41

atgataaaga?aaaccttatg?gaaggatatt?ttgagagcta?tcaagaatag?taaagggcga?60

ttcatttcct?tatttttcct?aatggcctta?ggctcctttg?ctttagttgg?acttaaagtc?120

accggtccag?atatggaacg?aacagccagc?cgttacctcg?aaagacacca?ggtgatggac?180

ctaacggtac?tggcttctca?tcaattttcc?caagccgata?aacaagagtt?agatacgtta?240

aaaggggctc?atttagaata?tggtcattta?cttgatgtca?gtctaactag?caaccagaaa?300

tctcttaggc?tctatagcgt?gccaaagaaa?gtgtctaagc?cagtcttggt?taaagggagt?360

tggccaaagc?gagagacaga?tttggtttta?tcctcctcac?ttgctaaaaa?ctatcagatt?420

ggagatgaac?tagcagtcac?ctcacctatg?gaaggtttgc?tgacgacaac?ccattttcaa?480

gtggttggtt?ttgcgaattc?ttcagaagtt?tggtccaagt?ccaatttagg?aagttcctca?540

accggagatg?gaagtctcta?cgcttatgct?tttgttaatc?caaacgtttt?taaaagtgcc?600

tttaacctgc?tgcgcatccg?ttttagtcat?cttcgtctga?caaacgcttt?ttccaaagac?660

tatcaaaaaa?gggtaaccca?aaaccaagct?catttggata?atctacttaa?agataatggc?720

cagaagcgtt?atgatgacct?ccaaaatcag?tatgaccttg?ccttaaaaaa?tggcagagca?780

gcacttgcaa?aggaaacagt?gaaactagct?gcgagtgagg?agaacttaac?ttttttagaa?840

ggctctgctt?tacaagaagc?taagcatcag?attgaacaag?gcaaacaagc?attagccaag?900

gaggaaaagc?agttagagca?ggtgcaggct?acaaaagata?agctagaaaa?acccagttac?960

ctgacttata?atcgctcgac?cctaccagga?ggagaaggat?atcatactta?tgcaacttca?1020

acgacctcca?tttcaaatgt?tggaaatatt?tttcctgttg?ttctttatct?cgtagctgct?1080

ctagtagcct?ttaccaccat?gacacgttat?gttgatgaag?aaagaacaag?ctctggtcta?1140

ttaaaagcca?ttggttattc?taacaaggat?atcagtttaa?agtttcttat?ctatgggctt?1200

ttagccagtt?ttttagggac?aactttaggt?attattgggg?gaacttacct?cttatctacc?1260

ttgatttcag?agatcttaac?aggagctttg?actattggaa?agactcacct?ttatagttat?1320

tggttttata?atggcatagc?ttacttgctg?gctatgttat?ctgctgtttt?accagcctac?1380

ttaattgtca?aaaaggaatt?attcctcaat?gcagctcagt?tattgctgcc?caaacctcct?1440

agtaaggggg?caaaaatctg?gttggaacac?cttacttttg?tctggaaagc?cctgtccttt?1500

actcacaagg?tgaccatacg?taatattttt?cgctataaac?aaagaatgct?gatgaccatt?1560

gtaggcgttg?caggctcagt?agccctttta?tttgcaggct?tagggattca?gtcgtcatta?1620

gccaaagtag?ttgagcatca?atttggtgat?ttaacgactt?atgatatttt?ggctgtcggt?1680

tcggccaaag?cgacagcgac?agagcaaact?gacttagcta?gctatcttaa?acaagaacct?1740

attacagggt?accaaaaggt?atcttatgcc?agcttaaccc?ttcctgtaaa?gggattacct?1800

gataagcaaa?gtatttccat?tttatcaagt?tcagctactt?ctcttagtcc?ctattttaat?1860

ctgctggata?gtcaggagca?aaagaaggtt?cccattccaa?cctctggtgt?tttgatttct?1920

gagaaattag?cctcctatta?caaggtaaaa?ccaggtgatc?agttggtatt?gactgatcgg?1980

aaaggacagt?cttataaagt?gacgattaaa?caggttattg?acatgacagt?tggccattac?2040

ctgataatgt?ctgataccta?ttttaagaat?cattttaaag?gattggaggc?tgctcctgcc?2100

tatctgatta?aggtaaaaga?caaagatagc?aagcacataa?aggagacagc?cagtgacttg?2160

ttaaccttaa?aagcgattag?agcagtttca?caaaacgtca?atcatattaa?atctgttcag?2220

ctagtagtca?cctctcttaa?tcaggtcatg?accctccttg?tcttcttgtc?tattttatta?2280

gcaatcgtta?tcctttataa?cttaacgact?attaatattg?ctgagcgtat?tcgagaatta?2340

tccactatta?aagttctggg?attttacgat?caggaggtca?ccttatatat?ttatcgagaa?2400

actatttcgc?tatccctagt?aggcattctt?ttaggtatct?atttaggaaa?aggcctgcat?2460

acttatatca?tgacaatgat?ttcaactggg?gatattcaat?ttggtgtaaa?ggttgatgct?2520

tatgtttatc?tagtgccaat?tctagtaatc?cttagcttgt?tagcggtatt?aggtatctgg?2580

gttaatcgcc?atttaaaaaa?ggttgatatg?ttagaagctt?tgaaatccat?agattga 2637

<210>42

<211>878

<212>PRT

＜213〉streptococcus pyogenes

<400>42

Met?Ile?Lys?Lys?Thr?Leu?Trp?Lys?Asp?Ile?Leu?Arg?Ala?Ile?Lys?Asn

1 5 10 15

Ser?Lys?Gly?Arg?Phe?Ile?Ser?Leu?Phe?Phe?Leu?Met?Ala?Leu?Gly?Ser

20 25 30

Phe?Ala?Leu?Val?Gly?Leu?Lys?Val?Thr?Gly?Pro?Asp?Met?Glu?Arg?Thr

35 40 45

Ala?Ser?Arg?Tyr?Leu?Glu?Arg?His?Gln?Val?Met?Asp?Leu?Thr?Val?Leu

50 55 60

Ala?Ser?His?Gln?Phe?Ser?Gln?Ala?Asp?Lys?Gln?Glu?Leu?Asp?Thr?Leu

65 70 75 80

Lys?Gly?Ala?His?Leu?Glu?Tyr?Gly?His?Leu?Leu?Asp?Val?Ser?Leu?Thr

85 90 95

Ser?Asn?Gln?Lys?Ser?Leu?Arg?Leu?Tyr?Ser?Val?Pro?Lys?Lys?Val?Ser

100 105 110

Lys?Pro?Val?Leu?Val?Lys?Gly?Ser?Trp?Pro?Lys?Arg?Glu?Thr?Asp?Leu

115 120 125

Val?Leu?Ser?Ser?Ser?Leu?Ala?Lys?Asn?Tyr?Gln?Ile?Gly?Asp?Glu?Leu

130 135 140

Ala?Val?Thr?Ser?Pro?Met?Glu?Gly?Leu?Leu?Thr?Thr?Thr?His?Phe?Gln

145 150 155 160

Val?Val?Gly?Phe?Ala?Asn?Ser?Ser?Glu?Val?Trp?Ser?Lys?Ser?Asn?Leu

165 170 175

Gly?Ser?Ser?Ser?Thr?Gly?Asp?Gly?Ser?Leu?Tyr?Ala?Tyr?Ala?Phe?Val

180 185 190

Asn?Pro?Asn?Val?Phe?Lys?Ser?Ala?Phe?Asn?Leu?Leu?Arg?Ile?Arg?Phe

195 200 205

Ser?His?Leu?Arg?Leu?Thr?Asn?Ala?Phe?Ser?Lys?Asp?Tyr?Gln?Lys?Arg

210 215 220

Val?Thr?Gln?Asn?Gln?Ala?His?Leu?Asp?Asn?Leu?Leu?Lys?Asp?Asn?Gly

225 230 235 240

Gln?Lys?Arg?Tyr?Asp?Asp?Leu?Gln?Asn?Gln?Tyr?Asp?Leu?Ala?Leu?Lys

245 250 255

Asn?Gly?Arg?Ala?Ala?Leu?Ala?Lys?Glu?Thr?Val?Lys?Leu?Ala?Ala?Ser

260 265 270

Glu?Glu?Asn?Leu?Thr?Phe?Leu?Glu?Gly?Ser?Ala?Leu?Gln?Glu?Ala?Lys

275 280 285

His?Gln?Ile?Glu?Gln?Gly?Lys?Gln?Ala?Leu?Ala?Lys?Glu?Glu?Lys?Gln

290 295 300

Leu?Glu?Gln?Val?Gln?Ala?Thr?Lys?Asp?Lys?Leu?Glu?Lys?Pro?Ser?Tyr

305 310 315 320

Leu?Thr?Tyr?Asn?Arg?Ser?Thr?Leu?Pro?Gly?Gly?Glu?Gly?Tyr?His?Thr

325 330 335

Tyr?Ala?Thr?Ser?Thr?Thr?Ser?Ile?Ser?Asn?Val?Gly?Asn?Ile?Phe?Pro

340 345 350

Val?Val?Leu?Tyr?Leu?Val?Ala?Ala?Leu?ValAla?Phe?Thr?Thr?Met?Thr

355 360 365

Arg?Tyr?Val?Asp?Glu?Glu?Arg?Thr?Ser?Ser?Gly?Leu?Leu?Lys?Ala?Ile

370 375 380

Gly?Tyr?Ser?Asn?Lys?Asp?Ile?Ser?Leu?Lys?Phe?Leu?Ile?Tyr?Gly?Leu

385 390 395 400

Leu?Ala?Ser?Phe?Leu?Gly?Thr?Thr?Leu?Gly?Ile?Ile?Gly?Gly?Thr?Tyr

405 410 415

Leu?Leu?Ser?Thr?Leu?Ile?Ser?Glu?Ile?Leu?Thr?Gly?Ala?Leu?Thr?Ile

420 425 430

Gly?Lys?Thr?His?Leu?Tyr?Ser?Tyr?Trp?Phe?Tyr?Asn?Gly?Ile?Ala?Tyr

435 440 445

Leu?Leu?Ala?Met?Leu?Ser?Ala?Val?Leu?Pro?Ala?Tyr?Leu?Ile?Val?Lys

450 455 460

Lys?Glu?Leu?Phe?Leu?Asn?Ala?Ala?Gln?Leu?Leu?Leu?Pro?Lys?Pro?Pro

465 470 475 480

Ser?Lys?Gly?Ala?Lys?Ile?Trp?Leu?Glu?His?Leu?Thr?Phe?Val?Trp?Lys

485 490 495

Ala?Leu?Ser?Phe?Thr?His?Lys?Val?Thr?Ile?Arg?Asn?Ile?Phe?Arg?Tyr

500 505 510

Lys?Gln?Arg?Met?Leu?Met?Thr?Ile?Val?Gly?Val?Ala?Gly?Ser?Val?Ala

515 520 525

Leu?Leu?Phe?Ala?Gly?Leu?Gly?Ile?Gln?Ser?Ser?Leu?Ala?Lys?Val?Val

530 535 540

Glu?His?Gln?Phe?Gly?Asp?Leu?Thr?Thr?Tyr?Asp?Ile?Leu?Ala?Val?Gly

545 550 555 560

Ser?Ala?Lys?Ala?Thr?Ala?Thr?Glu?Gln?Thr?Asp?Leu?Ala?Ser?Tyr?Leu

565 570 575

Lys?Gln?Glu?Pro?Ile?Thr?Gly?Tyr?Gln?Lys?Val?Ser?Tyr?Ala?Ser?Leu

580 585 590

Thr?Leu?Pro?Val?Lys?Gly?Leu?Pro?Asp?Lys?Gln?Ser?Ile?Ser?Ile?Leu

595 600 605

Ser?Ser?Ser?Ala?Thr?Ser?Leu?Ser?Pro?Tyr?Phe?Asn?Leu?Leu?Asp?Ser

610 615 620

Gln?Glu?Gln?Lys?Lys?Val?Pro?Ile?Pro?Thr?Ser?Gly?Val?Leu?Ile?Ser

625 630 635 640

Glu?Lys?Leu?Ala?Ser?Tyr?Tyr?Lys?Val?Lys?Pro?Gly?Asp?Gln?Leu?Val

645 650 655

Leu?Thr?Asp?Arg?Lys?Gly?Gln?Ser?Tyr?Lys?Val?Thr?Ile?Lys?Gln?Val

660 665 670

Ile?Asp?Met?Thr?Val?Gly?His?Tyr?Leu?Ile?Met?Ser?Asp?Thr?Tyr?Phe

675 680 685

Lys?Asn?His?Phe?Lys?Gly?Leu?Glu?Ala?Ala?Pro?Ala?Tyr?Leu?Ile?Lys

690 695 700

Val?Lys?Asp?Lys?Asp?Ser?Lys?His?Ile?Lys?Glu?Thr?Ala?Ser?Asp?Leu

705 710 715 720

Leu?Thr?Leu?Lys?Ala?Ile?Arg?Ala?Val?Ser?Gla?Asn?Val?Asn?His?Ile

725 730 735

Lys?Ser?Val?Gln?Leu?Val?ValThr?Ser?Leu?Asn?Gln?Val?Met?Thr?Leu

740 745 750

Leu?Val?Phe?Leu?Ser?Ile?Leu?Leu?Ala?Ile?Val?Ile?Leu?Tyr?Asn?Leu

755 760 765

Thr?Thr?Ile?Asn?Ile?Ala?Glu?Arg?Ile?Arg?Glu?Leu?Ser?Thr?Ile?Lys

770 775 780

Val?Leu?Gly?Phe?Tyr?Asp?Gln?Glu?Val?Thr?Leu?Tyr?Ile?Tyr?Arg?Glu

785 790 795 800

Thr?Ile?Ser?Leu?Ser?Leu?Val?Gly?Ile?Leu?Leu?Gly?Ile?Tyr?Leu?Gly

805 810 815

Lys?Gly?Leu?His?Thr?Tyr?Ile?Met?Thr?Met?Ile?Ser?Thr?Gly?Asp?Ile

820 825 830

Gln?Phe?Gly?Val?Lys?Val?Asp?Ala?Tyr?Val?Tyr?Leu?Val?Pro?Ile?Leu

835 840 845

Val?Ile?Leu?Ser?Leu?Leu?Ala?Val?Leu?Gly?Ile?Trp?Val?Asn?Arg?His

850 855 860

Leu?Lys?Lys?Val?Asp?Met?Leu?Glu?Ala?Leu?Lys?Ser?Ile?Asp

865 870 875

<210>43

<211>1344

<212>DNA

＜213〉streptococcus pyogenes

<400>43

atgatgttaa?cgaaggggca?acttccctat?agtgctgctt?ttacaacagg?agggctcttt?60

tactttgtta?tcattgcatt?aagttattat?ttgggatcga?cactttggct?agtctttgtt?120

caggtgtttt?gtttttactt?atctggttta?tatctttata?aactcattaa?ttatatgaca?180

ggctttcaaa?aagtggcttt?aactttttca?attagctact?atttattatc?tgttagtctt?240

ggttttgggg?gattgtatcc?tactcaactg?gctatgccat?ttatattaat?atcggcttgg?300

tttttaacta?agtattttgc?ctgtttagtg?aaagatgagg?catttattct?ttttggcttt?360

gtaggtgctc?ttgcaatgct?aattgacccg?agtaccctta?tcttttggtc?ttttgcttgt?420

gtgacagttt?tttcttataa?tataagccaa?aagcatcttg?caagaggttt?ttatcaactg?480

ctagcttcga?tttttggaat?gattttagtt?ttttacacag?caggatattt?cattttgaac?540

ttacaagtgc?taaatcctta?tttatcacaa?acgatgattt?atccttttac?tttttttaaa?600

tcaggaaact?tatcgttgct?ttttggactg?gctattcagt?tgttcttcgc?tttggggctt?660

ggtcttttga?cgggaatgga?gaatgtcatt?aggcgattta?aaaacaattc?tgatagggtc?720

gtcaagtggc?tatttgtcat?ggtcattcta?gaatctatac?ttgtggctat?attttcacaa?780

gactatcgcc?cctatcatct?tttacctctt?ttaccttttg?gattaatttt?gactgctatt?840

cctgttggct?atcagtatgg?tataggatta?ggtcagagta?gtcatcgcag?acgtcatggt?900

aaaaatggtg?ttggtcgagt?aatgatgatt?tatcttaaga?gacactttta?tttgccaatt?960

ttaattgtag?ggacaatact?aatctgttct?acttattgtt?tcattagtag?tattcctctt?1020

aatcaggagc?gtgatcatat?tgctagttat?ttagaacaga?aactaaataa?aactcaatct?1080

atttatgttt?gggatgatac?ttctaaaatt?tatttggaca?gtaaagctaa?atctgtttct?1140

caatttagtt?ctcctgacat?caatacgcaa?aaagagagtc?atcgaaaaat?attagaagat?1200

gaactattag?aaaataaggc?tgcttatatc?gttgttaatc?gctataaaaa?cctgcctaaa?1260

atcattcaaa?aagtattatc?tactaattac?aaagtagata?aacagataac?gacaaaaagt?1320

tttattgttt?atcagaaaaa?gtaa 1344

<210>44

<211>447

<212>PRT

＜213〉streptococcus pyogenes

<400>44

Met?Met?Leu?Thr?Lys?Gly?Gln?Leu?Pro?Tyr?Ser?Ala?Ala?Phe?Thr?Thr

1 5 10 15

Gly?Gly?Leu?Phe?Tyr?Phe?Val?Ile?Ile?Ala?Leu?Ser?Tyr?Tyr?Leu?Gly

20 25 30

Ser?Thr?Leu?Trp?Leu?Val?Phe?Val?Gln?Val?Phe?Cys?Phe?Tyr?Leu?Ser

35 40 45

Gly?Leu?Tyr?Leu?Tyr?Lys?Leu?Ile?Asn?Tyr?Met?Thr?Gly?Phe?Gln?Lys

50 55 60

Val?Ala?Leu?Thr?Phe?Ser?Ile?Ser?Tyr?Tyr?Leu?Leu?Ser?Val?Ser?Leu

65 70 75 80

Gly?Phe?Gly?Gly?Leu?Tyr?Pro?Thr?Gln?Leu?Ala?Met?Pro?Phe?Ile?Leu

85 90 95

Ile?Ser?Ala?Trp?Phe?Leu?Thr?Lys?Tyr?Phe?Ala?Cys?Leu?Val?Lys?Asp

100 105 110

Glu?Ala?Phe?Ile?Leu?Phe?Gly?Phe?Val?Gly?Ala?Leu?Ala?Met?Leu?Ile

115 120 125

Asp?Pro?Ser?Thr?Leu?Ile?Phe?Trp?Ser?Phe?Ala?Cys?Val?Thr?Val?Phe

130 135 140

Ser?Tyr?Asn?Ile?Ser?Gln?Lys?His?Leu?Ala?Arg?Gly?Phe?Tyr?Gln?Leu

145 150 155 160

Leu?Ala?Ser?Ile?Phe?Gly?Met?Ile?Leu?Val?Phe?Tyr?Thr?Ala?Gly?Tyr

165 170 175

Phe?Ile?Leu?Asn?Leu?Gln?Val?Leu?Asn?Pro?Tyr?Leu?Ser?Gln?Thr?Met

180 185 190

Ile?Tyr?Pro?Phe?Thr?Phe?Phe?Lys?Ser?Gly?Asn?Leu?Ser?Leu?Leu?Phe

195 200 205

Gly?Leu?Ala?Ile?Gln?Leu?Phe?Phe?Ala?Leu?Gly?Leu?Gly?Leu?Leu?Thr

210 215 220

Gly?Met?Glu?Asn?Val?Ile?Arg?Arg?Phe?Lys?Asn?Asn?Ser?Asp?Arg?Val

225 230 235 240

Val?Lys?Trp?Leu?Phe?Val?Met?Val?Ile?Leu?Glu?Ser?Ile?Leu?Val?Ala

245 250 255

Ile?Phe?Ser?Gln?Asp?Tyr?Arg?Pro?Tyr?His?Leu?Leu?Pro?Leu?Leu?Pro

260 265 270

Phe?Gly?Leu?Ile?Leu?Thr?Ala?Ile?Pro?Val?Gly?Tyr?Gln?Tyr?Gly?Ile

275 280 285

Gly?Leu?Gly?Gln?Ser?Ser?His?Arg?Arg?Arg?His?Gly?Lys?Asn?Gly?Val

290 295 300

Gly?Arg?Val?Met?Met?Ile?Tyr?Leu?Lys?Arg?His?Phe?Tyr?Leu?Pro?Ile

305 310 315 320

Leu?Ile?Val?Gly?Thr?Ile?Leu?Ile?Cys?Ser?Thr?Tyr?Cys?Phe?Ile?Ser

325 330 335

Ser?Ile?Pro?Leu?Asn?Gln?Glu?Arg?Asp?His?Ile?Ala?Ser?Tyr?Leu?Glu

340 345 350

Gln?Lys?Leu?Asn?Lys?Thr?Gln?Ser?Ile?Tyr?Val?Trp?Asp?Asp?Thr?Ser

355 360 365

Lys?Ile?Tyr?Leu?Asp?Ser?Lys?Ala?Lys?Ser?Val?Ser?Gln?Phe?Ser?Ser

370 375 380

Pro?Asp?Ile?Asn?Thr?Gln?Lys?Glu?Ser?His?Arg?Lys?Ile?Leu?Glu?Asp

385 390 395 400

Glu?Leu?Leu?Glu?Asn?Lys?Ala?Ala?Tyr?Ile?Val?Val?Asn?Arg?Tyr?Lys

405 410 415

Asn?Leu?Pro?Lys?Ile?Ile?Gln?Lys?Val?Leu?Ser?Thr?Asn?Tyr?Lys?Val

420 425 430

Asp?Lys?Gln?Ile?Thr?Thr?Lys?Ser?Phe?Ile?Val?Tyr?Gln?Lys?Lys

435 440 445

<210>45

<211>324

<212>DNA

＜213〉streptococcus pyogenes

<400>45

atgcagtttt?tacaaaaatg?gtttaaacac?tctaaaaaag?agaagttgaa?agaatcctcg?60

tcccttagta?cagaaataga?accttctgaa?aactgggcaa?agattcccgc?ttatattcct?120

gcagacaaaa?gtgattacaa?aaaggttact?ttaattacca?gtgttattgc?tgctgggcga?180

tagacctaat?agtcaattca?aggttaaacg?cattctcaag?cgcaatcctg?aggcaatcac?240

tgtttcttta?attgcttcaa?gtatcgctgc?aggtgtttac?ccagaaagtc?agtttcgggt?300

gacatccatc?tattgcaaaa?gtaa 324

<210>46

<211>107

<212>PRT

＜213〉streptococcus pyogenes

<400>46

Met?Gln?Phe?Leu?Gln?Lys?Trp?Phe?Lys?His?Ser?Lys?Lys?Glu?Lys?Leu

1 5 10 15

Lys?Glu?Ser?Ser?Pro?Leu?Ser?Thr?Glu?Ile?Glu?Pro?Ser?Glu?Asn?Trp

20 25 30

Glu?Lys?Ile?Pro?Ala?Tyr?Ile?Pro?Ala?Asp?Lys?Ser?Asp?Tyr?Lys?Lys

35 40 45

Val?Thr?Leu?Ile?Thr?Ser?Ala?Ile?Ala?Ala?Gly?Asp?Arg?Pro?Asn?Ser

50 55 60

Gln?Phe?Lys?Val?Lys?Arg?Ile?Leu?Lys?Arg?Asn?Pro?Glu?Ala?Ile?Thr

65 70 75 80

Val?Ser?Leu?Ile?Ala?Ser?Ser?Ile?Ala?Ala?Gly?Val?Tyr?Pro?Glu?Ser

85 90 95

Gln?Phe?Arg?Val?Thr?Ser?Ile?Tyr?Cys?Lys?Arg

100 105

<210>47

<211>288

<212>DNA

＜213〉streptococcus pyogenes

<400>47

atgacaaaac?gtaattcaaa?agcctatata?ttatggcaaa?aaattatcaa?aattttagga?60

attattgcac?taattggtac?ttttttcctc?gctttttggc?tgtatagact?aggtatatta?120

aacgatagta?atgctcttaa?agacttagtg?cagcgctata?gattatgggg?gccgtttgtt?180

tttattgtcg?tgcagatcat?acagattgtt?tttcctgtga?ttcctggagg?ccttacgaca?240

gttgcaggtt?ttttaatctt?tggcccctgt?aacaggtttt?atttataa 288

<210>48

<211>95

<212>PRT

＜213〉streptococcus pyogenes

<400>48

Met?Thr?Lys?Arg?Asn?Ser?Lys?Ala?Tyr?Ile?Leu?Trp?Gln?Lys?Ile?Ile

1 5 10 15

Lys?Ile?Leu?Gly?Ile?Ile?Ala?Leu?Ile?Gly?Thr?Phe?Phe?Leu?Ala?Phe

20 25 30

Trp?Leu?Tyr?Arg?Leu?Gly?Ile?Leu?Asn?Asp?Ser?Asn?Ala?Leu?Lys?Asp

35 40 45

Leu?Val?Gln?Arg?Tyr?Arg?Leu?Trp?Gly?Pro?Phe?Val?Phe?Ile?Val?Val

50 55 60

Gln?Ile?Ile?Gln?Ile?Val?Phe?Pro?Val?Ile?Pro?Gly?Gly?Leu?Thr?Thr

65 70 75 80

Val?Ala?Gly?Phe?Leu?Ile?Phe?Gly?Pro?Cys?Asn?Arg?Phe?Tyr?Leu

85 90 95

<210>49

<211>1311

<212>DNA

＜213〉streptococcus pyogenes

<400>49

atgaataaat?taaaaaaaga?gattttatca?gataactata?accacttttt?tcattttttt?60

gcggttttta?caggtatttt?tgtcattatg?actattatta?tcttacagat?tatgcggttt?120

ggcgtttatt?cgtcagttga?cagtagttta?gtttctgtta?gtaataatgc?aagtagctat?180

gctaatcgta?cgatggctag?aatatcttct?ttttactttg?atactgaaaa?taacattatt?240

aaggcgctgc?ctgattcaga?tagttctaag?ttattaggaa?cgcctgcagc?taatacagat?300

atcattttgt?ttagtgctaa?tggaacaatt?ttaaatgctt?ttgatgcgtt?ttctaactat?360

caaaattttc?atttagataa?acgccggttg?gggagtattg?aaaccaccag?tttaatgaat?420

ttttatggac?aagaagaaaa?ataccatacg?ataactgtag?gggttcatat?caaaaattat?480

cctgcagttg?cctatatgat?ggcagtagta?aatgtggaac?aattagaccg?cgctaatgaa?540

cgttatgagc?gcattattat?tatagttatg?agtgtttttt?ggctaatttc?tattttagca?600

agtatttatt?tagccaagtg?gagcagaaaa?cctattttag?aaagctatga?aaaacaaaaa?660

atgtttgttg?aaaatgctag?tcatgaatta?aggacccctt?tggcggtctt?acagaatcgt?720

ctggaatcgc?tttttcgtaa?gcccaacgaa?acgatattag?aaaatagtga?gcatctcgct?780

tctagtttag?acgaggttcg?caacatgcgc?atcttaacaa?ctaatttatt?aaatttagca?840

agacgagatg?atggcattaa?tccacagtgg?actcatttag?atacagattt?ttttaatgct?900

atttttgaga?attatgaact?agttgctaaa?gaatatggaa?aaatatttta?ttttcagaac?960

caagtcaata?gatcgttaag?aatggataag?gctttactaa?aacaattaat?aacgatttta?1020

tttgacaatg?ctattaaata?tacagataaa?aatggtatta?ttgaaattat?agtgaaaaca?1080

acggacaaaa?atttattaat?ttctgttatt?gataatggtc?cagggataac?agatgaagaa?1140

aagaaaaaga?tttttgatcg?tttttatcga?gttgacaaag?ctagaacacg?gcaaacaggt?1200

ggatttggct?tggggttggc?tttagctcag?caaatcgtga?tgtctttaaa?aggaaatatt?1260

acagtaaagg?ataatgatcc?taaaggtagt?atttttgaag?tcaaactata?a 1311

<210>50

<211>436

<212>PRT

＜213〉streptococcus pyogenes

<400>50

Met?Asn?Lys?Leu?Lys?Lys?Glu?Ile?Leu?Ser?Asp?Asn?Tyr?Asn?His?Phe

1 5 10 15

Phe?His?Phe?Phe?Ala?Val?Phe?Thr?Gly?Ile?Phe?Val?Ile?Met?Thr?Ile

20 25 30

Ile?Ile?Leu?Gln?Ile?Met?Arg?Phe?Gly?Val?Tyr?Ser?Ser?Val?Asp?Ser

35 40 45

Ser?Leu?Val?Ser?Val?Ser?Asn?Asn?Ala?Ser?Ser?Tyr?Ala?Asn?Arg?Thr

50 55 60

Met?Ala?Arg?Ile?Ser?Ser?Phe?Tyr?Phe?Asp?Thr?Glu?Asn?Asn?Ile?Ile

65 70 75 80

Lys?Ala?Leu?Pro?Asp?Ser?Asp?Ser?Ser?Lys?Leu?Leu?Gly?Thr?Pro?Ala

85 90 95

Ala?Asn?Thr?Asp?Ile?Ile?Leu?Phe?Ser?Ala?Asn?Gly?Thr?Ile?Leu?Asn

100 105 110

Ala?Phe?Asp?Ala?Phe?Ser?Asn?Tyr?Gln?Asn?Phe?His?Leu?Asp?Lys?Arg

115 120 125

Arg?Leu?Gly?Ser?Ile?Glu?Thr?Thr?Ser?Leu?Met?Asn?Phe?Tyr?Gly?Gln

130 135 140

Glu?Glu?Lys?Tyr?His?Thr?Ile?Thr?Val?Gly?Val?His?Ile?Lys?Asn?Tyr

145 150 155 160

Pro?Ala?Val?Ala?Tyr?Met?Met?Ala?Val?Val?Asn?Val?Glu?Gln?Leu?Asp

165 170 175

Arg?Ala?Asn?Glu?Arg?Tyr?Glu?Arg?Ile?Ile?Ile?Ile?Val?Met?Ser?Val

180 185 190

Phe?Trp?Leu?Ile?Ser?Ile?Leu?Ala?Ser?Ile?Tyr?Leu?Ala?Lys?Trp?Ser

195 200 205

Arg?Lys?Pro?Ile?Leu?Glu?Ser?Tyr?Glu?Lys?Gln?Lys?Met?Phe?Val?Glu

210 215 220

Asn?Ala?Ser?His?Glu?Leu?Arg?Thr?Pro?Leu?Ala?Val?Leu?Gln?Asn?Arg

225 230 235 240

Leu?Glu?Ser?Leu?Phe?Arg?Lys?Pro?Asn?Glu?Thr?Ile?Leu?Glu?Asn?Ser

245 250 255

Glu?His?Leu?Ala?Ser?Ser?Leu?Asp?Glu?Val?Arg?Asn?Met?Arg?Ile?Leu

260 265 270

Thr?Thr?Asn?Leu?Leu?Asn?Leu?Ala?Arg?Arg?Asp?Asp?Gly?Ile?Asn?Pro

275 280 285

Gln?Trp?Thr?His?Leu?Asp?Thr?Asp?Phe?Phe?Asn?Ala?Ile?Phe?Glu?Asn

290 295 300

Tyr?Glu?Leu?Val?Ala?Lys?Glu?Tyr?Gly?Lys?Ile?Phe?Tyr?Phe?Gln?Asn

305 310 315 320

Gln?Val?Asn?Arg?Ser?Leu?Arg?Met?Asp?Lys?Ala?Leu?Leu?Lys?Gln?Leu

325 330 335

Ile?Thr?Ile?Leu?Phe?Asp?Asn?Ala?Ile?Lys?Tyr?Thr?Asp?Lys?Asn?Gly

340 345 350

Ile?Ile?Glu?Ile?Ile?Val?Lys?Thr?Thr?Asp?Lys?Asn?Leu?Leu?Ile?Ser

355 360 365

Val?Ile?Asp?Asn?Gly?Pro?Gly?Ile?Thr?Asp?Glu?Glu?Lys?Lys?Lys?Ile

370 375 380

Phe?Asp?Arg?Phe?Tyr?Arg?Val?Asp?Lys?Ala?Arg?Thr?Arg?Gln?Thr?Gly

385 390 395 400

Gly?Phe?Gly?Leu?Gly?Leu?Ala?Leu?Ala?Gln?Gln?Ile?Val?Met?Ser?Leu

405 410 415

Lys?Gly?Asn?Ile?Thr?Val?Lys?Asp?Asn?Asp?Pro?Lys?Gly?Ser?Ile?Phe

420 425 430

Glu?Val?Lys?Leu

435

<210>51

<211>699

<212>DNA

＜213〉streptococcus pyogenes

<400>51

atgttttctg?gacatcaact?aaaaacagca?cgattatcaa?agggaataac?tcaatcagaa?60

ttgggaagat?tgttgcatgt?caataaaatg?acaatatcta?attgggaaaa?aggtaagaat?120

ataccaaatg?aaaaacattt?aaatgcctta?ttgcatctat?tcaatgtgac?atctgattat?180

ttcgacccaa?actatagatt?gctaacgcct?tataaccagc?tgacaatatc?taataaagaa?240

aaagtaattg?gctattcaga?gcgattgtta?aatcatcaaa?tagacaaaaa?atctaaagat?300

ctcatagata?aaccatcaca?attatatgct?tatcgggtct?atgaaagttt?atctgctggt?360

actggttact?cctattttgg?tgatggtaac?tttgatgttg?tcttttacga?tgaacaatta?420

gaatacgatt?ttgcgtcttg?ggtttttgga?gattctatgg?agccaactta?tttaaatggt?480

gaagttgttc?ttataaaaca?aaatagtttt?gattacgatg?gagcaattta?tgcagtcgaa?540

tgggatgggc?aaacatatat?caaaaaggta?tttcgtgaag?atgagggatt?acgtctagtg?600

tccttaaata?aaaaatattc?tgataagttt?gctccctata?gcgaagaacc?tcgcattatt?660

ggcaaaatta?tcgctaattt?taggccctta?gaaatttaa 699

<210>52

<211>232

<212>PRT

＜213〉streptococcus pyogenes

<400>52

Met?Phe?Ser?Gly?His?Gln?Leu?Lys?Thr?Ala?Arg?Leu?Ser?Lys?Gly?Ile

1 5 10 15

Thr?Gln?Ser?Glu?Leu?Gly?Arg?Leu?Leu?His?Val?Asn?Lys?Met?Thr?Ile

20 25 30

Ser?Asn?Trp?Glu?Lys?Gly?Lys?Asn?Ile?Pro?Asn?Glu?Lys?His?Leu?Asn

35 40 45

Ala?Leu?Leu?His?Leu?Phe?Asn?Val?Thr?Ser?Asp?Tyr?Phe?Asp?Pro?Asn

50 55 60

Tyr?Arg?Leu?Leu?Thr?Pro?Tyr?Asn?Gln?Leu?Thr?Ile?Ser?Asn?Lys?Glu

65 70 75 80

Lys?Val?Ile?Gly?Tyr?Ser?Glu?Arg?Leu?Leu?Asn?His?Gln?Ile?Asp?Lys

85 90 95

Lys?Ser?Lys?Asp?Leu?Ile?Asp?Lys?Pro?Ser?Gln?Leu?Tyr?Ala?Tyr?Arg

100 105 110

Val?Tyr?Glu?Ser?Leu?Ser?Ala?Gly?Thr?Gly?Tyr?Ser?Tyr?Phe?Gly?Asp

115 120 125

Gly?Asn?Phe?Asp?Val?Val?Phe?Tyr?Asp?Glu?Gln?Leu?Glu?Tyr?Asp?Phe

130 135 140

Ala?Ser?Trp?Val?Phe?Gly?Asp?Ser?Met?Glu?Pro?Thr?Tyr?Leu?Asn?Gly

145 150 155 160

Glu?Val?Val?Leu?Ile?Lys?Gln?Asn?Ser?Phe?Asp?Tyr?Asp?Gly?Ala?Ile

165 170 175

Tyr?Ala?Val?Glu?Trp?Asp?Gly?Gln?Thr?Tyr?Ile?Lys?Lys?Val?Phe?Arg

180 185 190

Glu?Asp?Glu?Gly?Leu?Arg?Leu?Val?Ser?Leu?Asn?Lys?Lys?Tyr?Ser?Asp

195 200 205

Lys?Phe?Ala?Pro?Tyr?Ser?Glu?Glu?Pro?Arg?Ile?Ile?Gly?Lys?Ile?Ile

210 215 220

Ala?Asn?Phe?Arg?Pro?Leu?Glu?Ile

225 230

<210>53

<211>1497

<212>DNA

＜213〉streptococcus pyogenes

<400>53

ttgaaaaggg?ttcccgtaga?aagagacaaa?cgcttagtct?ctttcttttt?ctttcttctc?60

atttctgtta?taataagtca?ggtaactaca?ttagaaagat?ttgtgggtct?gtcatctaag?120

agatgtcatt?cccgaagagg?tattagtgac?ttgtctcaat?caactgcaac?ttatattaat?180

gttattggag?ctgggctagc?tggttctgaa?gctgcctatc?agattgctaa?gcgcggtatc?240

cccgttaaat?tgtatgaaat?gcgtggtgtc?aaagcaacac?cgcaacataa?aaccactaat?300

tttgccgaat?tggtctgttc?caactcattt?cgtggtgata?gcttaaccaa?tgcagtcggt?360

cttctcaaag?aagaaatgcg?gcgattagac?tccattatta?tgcgtaatgg?tgaagctaac?420

cgcgtacctg?ctgggggagc?aatggctgtt?gaccgtgagg?ggtatgcaga?gagtgtcact?480

gcagagttgg?aaaatcatcc?tctcattgag?gtcattcgtg?gtgaaattac?agaaatccct?540

gacgatgcta?tcacggttat?cgcgacggga?ccgctgactt?cggatgccct?ggcagaaaaa?600

attcacgcgc?taaatggtgg?cgacggattc?tatttttacg?atgcagcagc?gcctatcatt?660

gataaatcta?ccattgatat?gagcaaggtt?taccttaaat?ctcgctacga?taaaggcgaa?720

gctgcttacc?tcaactgccc?tatgaccaaa?gaagaattca?tggctttcca?tgaagctctg?780

acaaccgcag?aagaagcccc?gctgaatgcc?tttgaaaaag?aaaagtattt?tgaaggctgt?840

atgccgattg?aagttatggc?taaacgtggc?attaaaacca?tgctttatgg?acctatgaaa?900

cccgttggat?tggaatatcc?agatgactat?acaggtcctc?gcgatggaga?atttaaaacg?960

ccatatgccg?tcgtgcaatt?gcgtcaagat?aatgcagctg?gaagccttta?taatatcgtt?1020

ggtttccaaa?cccatctcaa?atggggtgag?caaaaacgcg?ttttccaaat?gattccaggg?1080

cttgaaaatg?ctgagtttgt?ccgctacggc?gtcatgcatc?gcaattccta?tatggattca?1140

ccaaatcttt?taaccgaaac?cttccaatct?cggagcaatc?caaacctttt?ctttgcaggt?1200

cagatgactg?gagttgaagg?ttatgtcgaa?tcagctgctt?caggtttagt?agcaggaatc?1260

aatgctgctc?gtttgttcaa?aagagaagaa?gcacttattt?ttcctcagac?aacagctatt?1320

gggagtttgc?ctcattatgt?gactcatgcc?gacagtaagc?atttccaacc?aatgaacgtc?1380

aactttggca?tcatcaaaga?gttagaaggc?ccacgcattc?gtgacaaaaa?agaacgttat?1440

gaagctattg?ctagtcgtgc?tttggcagat?ttagacacct?gcttagcgtc?gctttaa 1497

<210>54

<211>498

<212>PRT

＜213〉streptococcus pyogenes

<400>54

Leu?Lys?Arg?Val?Pro?Val?Glu?Arg?Asp?Lys?Arg?Leu?Val?Ser?Phe?Phe

1 5 10 15

Phe?Phe?Leu?Leu?Ile?Ser?Val?Ile?Ile?Ser?Gln?Val?Thr?Thr?Leu?Glu

20 25 30

Arg?Phe?Val?Gly?Leu?Ser?Ser?Lys?Arg?Cys?His?Ser?Arg?Arg?Gly?Ile

35 40 45

Ser?Asp?Leu?Ser?Gln?Ser?Thr?Ala?Thr?Tyr?Ile?Asn?Val?Ile?Gly?Ala

50 55 60

Gly?Leu?Ala?Gly?Ser?Glu?Ala?Ala?Tyr?Gln?Asn?Ala?Lys?Arg?Gly?Ile

65 70 75 80

Pro?Val?Lys?Leu?Tyr?Glu?Met?Arg?Gly?Val?Lys?Ala?Thr?Pro?Gln?His

85 90 95

Lys?Thr?Thr?Asn?Phe?Ala?Glu?Leu?Val?Cys?Ser?Asn?Ser?Phe?Arg?Gly

100 105 110

Asp?Ser?Leu?Thr?Asn?Ala?Val?Gly?Leu?Leu?Lys?Glu?Glu?Met?Arg?Arg

115 120 125

Leu?Asp?Ser?Ile?Ile?Met?Arg?Asn?Gly?Glu?Ala?Asn?Arg?Val?Pro?Ala

130 135 140

Gly?Gly?Ala?Met?Ala?Val?Asp?Arg?Glu?Gly?Tyr?Ala?Glu?Ser?Val?Thr

145 150 155 160

Ala?Glu?Leu?Glu?Asn?His?Pro?Leu?Ile?Glu?Val?Ile?Arg?Gly?Glu?Ile

165 170 175

Thr?Glu?Ile?Pro?Asp?Asp?Ala?Ile?Thr?Val?Ile?Ala?Thr?Gly?Pro?Leu

180 185 190

Thr?Ser?Asp?Ala?Leu?Ala?Glu?Lys?Ile?His?Ala?Leu?Asn?Gly?Gly?Asp

195 200 205

Gly?Phe?Tyr?Phe?Tyr?Asp?Ala?Ala?Ala?Pro?Ile?Ile?Asp?Lys?Ser?Thr

210 215 220

Ile?Asp?Met?Ser?Lys?Val?Tyr?Leu?Lys?Ser?Arg?Tyr?Asp?Lys?Gly?Glu

225 230 235 240

Ala?Ala?Tyr?Leu?Asn?Cys?Pro?Met?Thr?Lys?Glu?Glu?Phe?Met?Ala?Phe

245 250 255

His?Glu?Ala?Leu?Thr?Thr?Ala?Glu?Glu?Ala?Pro?Leu?Asn?Ala?Phe?Glu

260 265 270

Lys?Glu?Lys?Tyr?Phe?Glu?Gly?Cys?Met?Pro?Ile?Glu?Val?Met?Ala?Lys

275 280 285

Arg?Gly?Ile?Lys?Thr?Met?Leu?Tyr?Gly?Pro?Met?Lys?Pro?Val?Gly?Leu

290 295 300

Glu?Tyr?Pro?Asp?Asp?Tyr?Thr?Gly?Pro?Arg?Asp?Gly?Glu?Phe?Lys?Thr

305 310 315 320

Pro?Tyr?Ala?Val?Val?Gln?Leu?Arg?Gln?Asp?Asn?Ala?Ala?Gly?Ser?Leu

325 330 335

Tyr?Asn?Ile?Val?Gly?Phe?Gln?Thr?His?Leu?Lys?Trp?Gly?Glu?Gln?Lys

340 345 350

Arg?Val?Phe?Gln?Met?Ile?Pro?Gly?Leu?Glu?Asn?Ala?Glu?Phe?Val?Arg

355 360 365

Tyr?Gly?Val?Met?His?Arg?Asn?Ser?Tyr?Met?Asp?Ser?Pro?Asn?Leu?Leu

370 375 380

Thr?Glu?Thr?Phe?Gln?Ser?Arg?Ser?Asn?Pro?Asn?Leu?Phe?Phe?Ala?Gly

385 390 395 400

Gln?Met?Thr?Gly?Val?Glu?Gly?Tyr?Val?Glu?Ser?Ala?Ala?Ser?Gly?Leu

405 410 415

Val?Ala?Gly?Ile?Asn?Ala?Ala?Arg?Leu?Phe?Lys?Arg?Glu?Glu?Ala?Leu

420 425 430

Ile?Phe?Pro?Gln?Thr?Thr?Ala?Ile?Gly?Ser?Leu?Pro?His?Tyr?Val?Thr

435 440 445

His?Ala?Asp?Ser?Lys?His?Phe?Gln?Pro?Met?Asn?Val?Asn?Phe?Gly?Ile

450 455 460

Ile?Lys?Glu?Leu?Glu?Gly?Pro?Arg?Ile?Arg?Asp?Lys?Lys?Glu?Ara?Tyr

465 470 475 480

Glu?Ala?Ile?Ala?Ser?Arg?Ala?Leu?Ala?Asp?Leu?Asp?Thr?Cys?Leu?Ala

485 490 495

Ser?Leu

<210>55

<211>1539

<212>DNA

＜213〉streptococcus pyogenes

<400>55

atgattaagg?atatgattga?cagtattgag?cagtttgctc?agacacaggc?tgattttcca?60

gtttatgatt?gtttagggga?acgccgcact?tacggacaac?tcaaaagaga?ttctgatagc?120

attgctgcat?ttatagatag?cttagcttta?ctggcaaaat?ctccagtttt?ggtttttggg?180

gcgcaaactt?atgatatgtt?agctactttt?gtagctttaa?ccaaatctgg?tcatgcctat?240

atcccagtag?atgtgcattc?agcaccagaa?cgtattttag?cgattattga?gattgcaaag?300

cccagtttaa?tcattgctat?tgaggaattc?cctcttacta?ttgaagggat?ttcccttgtc?360

tcactatcag?agattgagtc?agcaaaatta?gcagaaatgc?cctatgagcg?aacacattct?420

gttaaaggag?atgataatta?ttacattatt?ttcacctctg?gaacaacggg?ccaaccaaaa?480

ggggtgcaaa?tttcacatga?caatcttttg?agctttacca?actggatgat?cgaagatgca?540

gcatttgatg?tacctaaaca?gccacaaatg?ctggcgcagc?caccctattc?ctttgacctt?600

tctgtcatgt?actgggcacc?taccttggct?ttaggtggaa?ccttatttgc?acttccaaaa?660

gaattggtgg?cagactttaa?acagttattt?acaacgattg?cgcaacttcc?agttggcatt?720

tggacatcga?ctccttcatt?tgctgatatg?gccatgctta?gcgatgattt?ttgccaggct?780

aagatgccag?ctttaacgca?tttttatttt?gatggcgaag?aattaaccgt?ttctacggct?840

cgaaaactct?ttgaacgttt?cccgagtgcc?aagattatca?atgcctatgg?accaacagaa?900

gcgactgtag?ccttatccgc?tattgaaatt?accagagaaa?tggtggataa?ttatacccgc?960

ttgccgattg?gctaccccaa?accagattct?ccgacctata?ttattgatga?agatggtaag?1020

gagttatctt?caggggagca?gggtgaaatt?attgttacag?ggcctgctgt?gtcaaaaggt?1080

tacctcaaca?atcctgagaa?aaccgcagaa?gctttcttta?cttttaaagg?acagcctgct?1140

tatcatacag?gggacattgg?ctcactgaca?gaagataata?ttttgttgta?tggtgggcgt?1200

ttggattttc?agattaaata?tgctggttat?cgtattgaac?tagaagatgt?ttcccaacag?1260

ttgaatcaat?caccaatggt?agcatcggct?gtggctgttc?caaggtacaa?taaagaacat?1320

aaggttcaaa?atctcttggc?ctatatcgtt?gtcaaagatg?gggttaaaga?gcgttttgat?1380

agggaattgg?agttaaccaa?agctatcaaa?gcctctgtta?aggatcatat?gatgagttat?1440

atgatgcctt?ctaagtttct?ttatcgagat?agtctaccat?taactccaaa?tggtaagata?1500

gatattaaaa?ctttgattaa?tgaggtcaat?aaccgataa 1539

<210>56

<211>512

<212>PRT

＜213〉streptococcus pyogenes

<400>56

Met?Ile?Lys?Asp?Met?Ile?Asp?Ser?Ile?Glu?Gln?Phe?Ala?Gln?Thr?Gln

1 5 10 15

Ala?Asp?Phe?Pro?Val?Tyr?Asp?Cys?Leu?Gly?Glu?Arg?Arg?Thr?Tyr?Gly

20 25 30

Gln?Leu?Lys?Arg?Asp?Ser?Asp?Ser?Ile?Ala?Ala?Phe?Ile?Asp?Ser?Leu

35 40 45

Ala?Leu?Leu?Ala?Lys?Ser?Pro?Val?Leu?Val?Phe?Gly?Ala?Gln?Thr?Tyr

50 55 60

Asp?Met?Leu?Ala?Thr?Phe?Val?Ala?Leu?Thr?Lys?Ser?Gly?His?Ala?Tyr

65 70 75 80

Ile?Pro?Val?Asp?Val?His?Ser?Ala?Pro?Glu?Arg?Ile?Leu?Ala?Ile?Ile

85 90 95

Glu?Ile?Ala?Lys?Pro?Ser?Leu?Ile?Ile?Ala?Ile?Glu?Glu?Phe?Pro?Leu

100 105 110

Thr?Ile?Glu?Gly?Ile?Ser?Leu?Val?Ser?Leu?Ser?Glu?Ile?Glu?Ser?Ala

115 120 125

Lys?Leu?Ala?Glu?Met?Pro?Tyr?Glu?Arg?Thr?His?Ser?Val?Lys?Gly?Asp

130 135 140

Asp?Asn?Tyr?Tyr?Ile?Ile?Phe?Thr?Ser?Gly?Thr?Thr?Gly?Gln?Pro?Lys

145 150 155 160

Gly?Val?Gln?Ile?Ser?His?Asp?Asn?Leu?Leu?Ser?Phe?Thr?Asn?Trp?Met

165 170 175

Ile?Glu?Asp?Ala?Ala?Phe?Asp?Val?Pro?Lys?Gln?Pro?Gln?Met?Leu?Ala

180 185 190

Gln?Pro?Pro?Tyr?Ser?Phe?Asp?Leu?Ser?Val?Met?Tyr?Trp?Ala?Pro?Thr

195 200 205

Leu?Ala?Leu?Gly?Gly?Thr?Leu?Phe?Ala?Leu?Pro?Lys?Glu?Leu?Val?Ala

210 215 220

Asp?Phe?Lys?Gln?Leu?Phe?Thr?Thr?Ile?Ala?Gln?Leu?Pro?Val?Gly?Ile

225 230 235 240

Trp?Thr?Ser?Thr?Pro?Ser?Phe?Ala?Asp?Met?Ala?Met?Leu?Ser?Asp?Asp

245 250 255

Phe?Cys?Gln?Ala?Lys?Met?Pro?Ala?Leu?Thr?His?Phe?Tyr?Phe?Asp?Gly

260 265 270

Glu?Glu?Leu?Thr?Val?Ser?Thr?Ala?Arg?Lys?Leu?Phe?Glu?Arg?Phe?Pro

275 280 285

Ser?Ala?Lys?Ile?Ile?Asn?Ala?Tyr?Gly?Pro?Thr?Glu?Ala?Thr?Val?Ala

290 295 300

Leu?Ser?Ala?Ile?Glu?Ile?Thr?Arg?Glu?Met?Val?Asp?Asn?Tyr?Thr?Arg

305 310 315 320

Leu?Pro?Ile?Gly?Tyr?Pro?Lys?Pro?Asp?Ser?Pro?Thr?Tyr?Ile?Ile?Asp

325 330 335

Glu?Asp?Gly?Lys?Glu?Leu?Ser?Ser?Gly?Glu?Gln?Gly?Glu?Ile?Ile?Val

340 345 350

Thr?Gly?Pro?Ala?Val?Ser?Lys?Gly?Tyr?Leu?Asn?Asn?Pro?Glu?Lys?Thr

355 360 365

Ala?Glu?Ala?Phe?Phe?Thr?Phe?Lys?Gly?Gln?Pro?Ala?Tyr?His?Thr?Gly

370 375 380

Asp?Ile?Gly?Ser?Leu?Thr?Glu?Asp?Asn?Ile?Leu?Leu?Tyr?Gly?Gly?Arg

385 390 395 400

Leu?Asp?Phe?Gln?Ile?Lys?Tyr?Ala?Gly?Tyr?Arg?Ile?Glu?Leu?Glu?Asp

405 410 415

Val?Ser?Gln?Gln?Leu?Asn?Gln?Ser?Pro?Met?Val?Ala?Ser?Ala?Val?Ala

420 425 430

Val?Pro?Arg?Tyr?Asn?Lys?Glu?His?Lys?Val?Gln?Asn?Leu?Leu?Ala?Tyr

435 440 445

Ile?Val?Val?Lys?Asp?Gly?Val?Lys?Glu?Arg?Phe?Asp?Arg?Glu?Leu?Glu

450 455 460

Leu?Thr?Lys?Ala?Ile?Lys?Ala?Ser?Val?Lys?Asp?His?Met?Met?Ser?Tyr

465 470 475 480

Met?Met?Pro?Ser?Lys?Phe?Leu?Tyr?Arg?Asp?Ser?Leu?Pro?Leu?Thr?Pro

485 490 495

Asn?Gly?Lys?Ile?Asp?Ile?Lys?Thr?Leu?Ile?Asn?Glu?Val?Asn?Asn?Arg

500 505 510

<210>57

<211>1278

<212>DNA

＜213〉streptococcus pyogenes

<400>57

atgacaaaaa?ctaatcttaa?ctggagcggt?ttttcaaaga?aaacattcga?agaacgcctc?60

caacttatcg?aaaaatttaa?actacttaat?gctgaaaact?taaatcaact?caaaacagac?120

gttcttttgc?ctatccaaac?agctaatcaa?atgactgaaa?atgtcttagg?acgattggct?180

ttgcccttta?gcatagctcc?tgattttctt?gtcaacggtt?caacttatca?gatgcctttt?240

gtcacggaag?aaccttctgt?tgttgctgca?gcatctttcg?cagcaaaact?aatcaaacgc?300

tcaggtggtt?ttaaagctca?aaccctaaac?cgacaaatga?ttggtcaaat?tgttctttac?360

gatatcgacc?aaatagataa?cgctaaagcc?gccatccttc?ataaaacaaa?aaagctaatt?420

gcattggcaa?ataaagctta?tccttccatt?gttaaaagag?gtggaggcgc?tagaaccatt?480

catttggaag?aaaaaggaga?atttttgatt?ttctatctga?ctgttgatac?ccaagaagct?540

atgggagcaa?atatggtcaa?tactatgatg?gaagctcttg?ttcctgattt?aacaagactg?600

tctaaggggc?attgtctaat?ggcgatttta?tctaattacg?caacagagtc?gcttgttact?660

actagttgtg?agattcccgt?gcgcctttta?gatcacgata?aaacaaaatc?cctacagtta?720

gctcaaaaaa?tagagctagc?cagccgacta?gctcaagtag?atccttaccg?ggctactact?780

cataataaag?gtatttttaa?tggcattgat?gcagtggtaa?tagccacagg?aaatgactgg?840

cgtgctattg?aagcaggggc?ccatgcttat?gcctcaagaa?atggtagcta?tcaaggactt?900

agtcagtggc?attttgacca?agataaacaa?gttctgcttg?gccaaatgac?cctccctatg?960

cctattgcta?gtaagggggg?atctatcggg?cttaacccta?ctgtttctat?cgcacatgat?1020

cttcttaatc?aacctgatgc?caaaacatta?gcccaattga?ttgcatctgt?ggggttagct?1080

caaaactttg?ctgcactaaa?agctctgacc?tcatctggca?tccaagctgg?tcacatgaaa?1140

ctacatgcga?aatcattagc?tcttttggcg?ggggcaaccc?aagacgaaat?tgctccttta?1200

gttaatgctt?tactagctga?taaaccaata?aatctagaaa?aagcacattt?ttacttatcc?1260

cagctaagac?agtcttaa 1278

<210>58

<211>425

<212>PRT

＜213〉streptococcus pyogenes

<400>58

Met?Thr?Lys?Thr?Asn?Leu?Asn?Trp?Ser?Gly?Phe?Ser?Lys?Lys?Thr?Phe

1 5 10 15

Glu?Glu?Arg?Leu?Gln?Leu?Ile?Glu?Lys?Phe?Lys?Leu?Leu?Asn?Ala?Glu

20 25 30

Asn?Leu?Asn?Gln?Leu?Lys?Thr?Asp?Val?Leu?Leu?Pro?Ile?Gln?Thr?Ala

35 40 45

Asn?Gln?Met?Thr?Glu?Asn?Val?Leu?Gly?Arg?Leu?Ala?Leu?Pro?Phe?Ser

50 55 60

Ile?Ala?Pro?Asp?Phe?Leu?Val?Asn?Gly?Ser?Thr?Tyr?Gln?Met?Pro?Phe

65 70 75 80

Val?Thr?Glu?Glu?Pro?Ser?Val?Val?Ala?Ala?Ala?Ser?Phe?Ala?Ala?Lys

85 90 95

Leu?Ile?Lys?Arg?Ser?Gly?Gly?Phe?Lys?Ala?Gln?Thr?Leu?Asn?Arg?Gln

100 105 110

Met?Ile?Gly?Gln?Ile?Val?Leu?Tyr?Asp?Ile?Asp?Gln?Ile?Asp?Asn?Ala

115 120 125

Lys?Ala?Ala?Ile?Leu?His?Lys?Thr?Lys?Lys?Leu?Ile?Ala?Leu?Ala?Asn

130 135 140

Lys?Ala?Tyr?Pro?Ser?Ile?Val?Lys?Arg?Gly?Gly?Gly?Ala?Arg?Thr?Ile

145 150 155 160

His?Leu?Glu?Glu?Lys?Gly?Glu?Phe?Leu?Ile?Phe?Tyr?Leu?Thr?Val?Asp

165 170 175

Thr?Gln?Glu?Ala?Met?Gly?Ala?Asn?Met?Val?Asn?Thr?Met?Met?Glu?Ala

180 185 190

Leu?Val?Pro?Asp?Leu?Thr?Arg?Leu?Ser?Lys?Gly?His?Cys?Leu?Met?Ala

195 200 205

Ile?Leu?Ser?Asn?Tyr?Ala?Thr?Glu?Ser?Leu?Val?Thr?Thr?Ser?Cys?Glu

210 215 220

Ile?Pro?Val?Arg?Leu?Leu?Asp?His?Asp?Lys?Thr?Lys?Ser?Leu?Gln?Leu

225 230 235 240

Ala?Gln?Lys?Ile?Glu?Leu?Ala?Ser?Arg?Leu?Ala?Gln?Val?Asp?Pro?Tyr

245 250 255

Arg?Ala?Thr?Thr?His?Asn?Lys?Gly?Ile?Phe?Asn?Gly?Ile?Asp?Ala?Val

260 265 270

Val?Ile?Ala?Thr?Gly?Asn?Asp?Trp?Arg?Ala?Ile?Glu?Ala?Gly?Ala?His

275 280 285

Ala?Tyr?Ala?Ser?Arg?Asn?Gly?Ser?Tyr?Gln?Gly?Leu?Ser?Gln?Trp?His

290 295 300

Phe?Asp?Gln?Asp?Lys?Gln?Val?Leu?Leu?Gly?Gln?Met?Thr?Leu?Pro?Met

305 310 315 320

Pro?Ile?Ala?Ser?Lys?Gly?Gly?Ser?Ile?Gly?Leu?Asn?Pro?Thr?Val?Ser

325 330 335

Ile?Ala?His?Asp?Leu?Leu?Asn?Gln?Pro?Asp?Ala?Lys?Thr?Leu?Ala?Gln

340 345 350

Leu?Ile?Ala?Ser?Val?Gly?Leu?Ala?Gln?Asn?Phe?Ala?Ala?Leu?Lys?Ala

355 360 365

Leu?Thr?Ser?Ser?Gly?Ile?Gln?Ala?Gly?His?Met?Lys?Leu?His?Ala?Lys

370 375 380

Ser?Leu?Ala?Leu?Leu?Ala?Gly?Ala?Thr?Gln?Asp?Glu?Ile?Ala?Pro?Leu

385 390 395 400

Val?Asn?Ala?Leu?Leu?Ala?Asp?Lys?Pro?Ile?Asn?Leu?Glu?Lys?Ala?His

405 410 415

Phe?Tyr?Leu?Ser?Gln?Leu?Arg?Gln?Ser

420 425

<210>59

<211>915

<212>DNA

＜213〉streptococcus pyogenes

<400>59

atggtattat?ttacaggaaa?aacagtcgaa?gaagctatcg?aaacaggact?tcaagagtta?60

gggttatcac?gccttaaagc?gcatatcaaa?gtcatttcaa?aagagaaaaa?aggtttcctc?120

gggtttggta?aaaagccagc?ccaagttgat?atcgaaggta?tcagcgacaa?gacggtttat?180

aaggctgata?aaaaagccac?tcgaggtgtt?ccagaggata?ttaatcgtca?aaatactccc?240

gcggtcaatt?ctgctgatgt?agaacctgaa?gaaataaaag?ccactcaaag?gttagaagca?300

gaagatacca?aagtggttcc?tctcatgtct?gaagatagtc?cagcgcaaac?cccttctaat?360

cttgctgaga?cagtcactga?aacaaaagca?caacaaccgt?caatcccagt?cgaagaatca?420

gaagtgcccc?aagatgctgg?taatgatggt?ttcagcaaag?atattgaaaa?agctgctcag?480

gaagtgtccg?attatgtgac?taaaattatc?tatgaaatgg?atatcgaagc?taccgttgaa?540

accagcaaca?atcgtcgcca?aatcaatttg?cagattgaaa?cgccagaggc?gggacgtgtg?600

attggttacc?atggtaaggt?cttgaaatcc?ttgcagttgt?tagctcaaaa?tttcttgcac?660

gaccgctatt?caaaaaattt?ttcagtatcc?ttgaatgtcc?atgattatgt?ggaacatcga?720

acagaaacct?tgattgactt?tacgcaaaaa?gttgccaaac?gcgttttgga?atctggtcaa?780

gattacacca?tggatcccat?gagtaacagt?gagcgtaaaa?tcgttcataa?aaccgtttca?840

tccattgaag?gggttgatag?ttattctgaa?ggcaatgacc?ctaaccgcta?tgtggtagtc?900

agtcttcaac?gttaa 915

<210>60

<211>304

<212>PRT

＜213〉streptococcus pyogenes

<400>60

Met?Val?Leu?Phe?Thr?Gly?Lys?Thr?Val?Glu?Glu?Ala?Ile?Glu?Thr?Gly

1 5 10 15

Leu?Gln?Glu?Leu?Gly?Leu?Ser?Arg?Leu?Lys?Ala?His?Ile?Lys?Val?Ile

20 25 30

Ser?Lys?Glu?Lys?Lys?Gly?Phe?Leu?Gly?Phe?Gly?Lys?Lys?Pro?Ala?Gln

35 40 45

Val?Asp?Ile?Glu?Gly?Ile?Ser?Asp?Lys?Thr?Val?Tyr?Lys?Ala?Asp?Lys

50 55 60

Lys?Ala?Thr?Arg?Gly?Val?Pro?Glu?Asp?Ile?Asn?Arg?Gln?Asn?Thr?Pro

65 70 75 80

Ala?Val?Asn?Ser?Ala?Asp?Val?Glu?Pro?Glu?Glu?Ile?Lys?Ala?Thr?Gln

85 90 95

Arg?Leu?Glu?Ala?Glu?Asp?Thr?Lys?Val?Val?Pro?Leu?Met?Ser?Glu?Asp

100 105 110

Ser?Pro?Ala?Gln?Thr?Pro?Ser?Asn?Leu?Ala?Glu?Thr?Val?Thr?Glu?Thr

115 120 125

Lys?Ala?Gln?Gln?Pro?Ser?Ile?Pro?Val?Glu?Glu?Ser?Glu?Val?Pro?Gln

130 135 140

Asp?Ala?Gly?Asn?Asp?Gly?Phe?Ser?Lys?Asp?Ile?Glu?Lys?Ala?Ala?Gln

145 150 155 160

Glu?Val?Ser?Asp?Tyr?Val?Thr?Lys?Ile?Ile?Tyr?Glu?Met?Asp?Ile?Glu

165 170 175

Ala?Thr?Val?Glu?Thr?Ser?Asn?Asn?Arg?Arg?Gln?Ile?Asn?Leu?Gln?Ile

180 185 190

Glu?Thr?Pro?Glu?Ala?Gly?Arg?Val?Ile?Gly?Tyr?His?Gly?Lys?Val?Leu

195 200 205

Lys?Ser?Leu?Gln?Leu?Leu?Ala?Gln?Asn?Phe?Leu?His?Asp?Arg?Tyr?Ser

210 215 220

Lys?Asn?Phe?Ser?Val?Ser?Leu?Asn?Val?His?Asp?Tyr?Val?Glu?His?Arg

225 230 235 240

Thr?Glu?Thr?Leu?Ile?Asp?Phe?Thr?Gln?Lys?Val?Ala?Lys?Arg?Val?Leu

245 250 255

Glu?Ser?Gly?Gln?Asp?Tyr?Thr?Met?Asp?Pro?Met?Ser?Asn?Ser?Glu?Arg

260 265 270

Lys?Ile?Val?His?Lys?Thr?Val?Ser?Ser?Ile?Glu?Gly?Val?Asp?Ser?Tyr

275 280 285

Ser?Glu?Gly?Asn?Asp?Pro?Asn?Arg?Tyr?Val?Val?Val?Ser?Leu?Gln?Arg

290 295 300

<210>61

<211>1269

<212>DNA

＜213〉streptococcus pyogenes

<400>61

atgacaacaa?aatatcaaac?aatcatttcc?aatatcgaac?aagatatcca?gaaacagcga?60

ctaaaaaaag?gggacaagct?accttcaatt?agggtactaa?gtaaggttta?ttattgtagc?120

aaagatactg?ttcagcgggc?tcttttagaa?ttaaaatatc?gtcacttaat?ttatgctgtt?180

cctaaaagtg?gttattatgt?tttgggtaac?gtaagcatgc?ccgataatgt?tcttaatcta?240

agtcttgaag?attataataa?tatggcttat?gaagatttcc?gactctgctt?aaatgaggcc?300

ttaagtgcta?aagacaaata?cctctttcat?tattatcaca?aaaccgaagg?actagaagaa?360

ctaagggaag?ctttgcttct?ttacttagct?gaaaatagtg?tttacagtaa?caaagaccaa?420

ctattaatca?cctctgggac?gcaacaagcg?ctttatattt?tatcacaaat?gccttttcct?480

aatacgggca?aaacaattct?ccttgaaaag?cctacttatc?atcggatgga?agctattgtt?540

gctcagttag?gattacccta?tcaaactatc?tctagacact?ttaatggcct?tgatttagaa?600

ctcttagaat?ctttatttca?aactggcgat?attaaatttt?tttacacgat?ttcacgattt?660

tcacatcctc?tgggactttc?ttatagcact?aaggaaaagg?aagctattgt?tcgtctagca?720

caacgatacc?aggtttatat?tttagaagat?gattatttag?gagattttgt?gaaacttaaa?780

gagccaccaa?ttcattatta?tgacactcat?caccgaatca?tctacctaaa?atctttttca?840

atgagtgttt?ttcctgcact?ccgtatcgga?gctctagttt?taccgtcagg?tttaaaacct?900

cattttttaa?cccaaaaatc?attgattgat?ttagacacga?acttactaat?gcaaaaagca?960

ttagctcttt?atttagaaaa?tgggatgttt?caaaaaaatc?ttcgctttat?taagcgttat?1020

ctaaaacagc?gggaacgtca?attggctctc?tttttaaaac?aaaattgccc?tgatattcac?1080

tatcaactaa?cccctactca?tttagtgatt?gattacacga?catcagactc?ttatagaaac?1140

tttacattag?ataaatctga?tagaataata?ataactggaa?aaaaacgcta?tctctctatt?1200

actatcaatc?aacaaataca?aagcaaatta?aactcactaa?tcaaaaatac?ttgtggaaag?1260

agcaactaa 1269

<210>62

<211>422

<212>PRT

＜213〉streptococcus pyogenes

<400>62

Met?Thr?Thr?Lys?Tyr?Gln?Thr?Ile?Ile?Ser?Asn?Ile?Glu?Gln?Asp?Ile

1 5 10 15

Gln?Lys?Gln?Arg?Leu?Lys?Lys?Gly?Asp?Lys?Leu?Pro?Ser?Ile?Arg?Val

20 25 30

Leu?Ser?Lys?Val?Tyr?Tyr?Cys?Ser?Lys?Asp?Thr?Val?Gln?Arg?Ala?Leu

35 40 45

Leu?Glu?Leu?Lys?Tyr?Arg?His?Leu?Ile?Tyr?Ala?Val?Pro?Lys?Ser?Gly

50 55 60

Tyr?Tyr?Val?Leu?Gly?Asn?Val?Ser?Met?Pro?Asp?Asn?Val?Leu?Asn?Leu

65 70 75 80

Ser?Leu?Glu?Asp?Tyr?Asn?Asn?Met?Ala?Tyr?Glu?Asp?Phe?Arg?Leu?Cys

85 90 95

Leu?Asn?Glu?Ala?Leu?Ser?Ala?Lys?Asp?Lys?Tyr?Leu?Phe?His?Tyr?Tyr

100 105 110

His?Lys?Thr?Glu?Gly?Leu?Glu?Glu?Leu?Arg?Glu?Ala?Leu?Leu?Leu?Tyr

115 120 125

Leu?Ala?Glu?Asn?Ser?Val?Tyr?Ser?Asn?Lys?Asp?Gln?Leu?Leu?Ile?Thr

130 135 140

Ser?Gly?Thr?Gln?Gln?Ala?Leu?Tyr?Ile?Leu?Ser?Gln?Met?Pro?Phe?Pro

145 150 155 160

Asn?Thr?Gly?Lys?Thr?Ile?Leu?Leu?Glu?Lys?Pro?Thr?Tyr?His?Arg?Met

165 170 175

Glu?Ala?Ile?Val?Ala?Gln?Leu?Gly?Leu?Pro?Tyr?Gln?Thr?Ile?Ser?Arg

180 185 190

His?Phe?Asn?Gly?Leu?Asp?Leu?Glu?Leu?Leu?Glu?Ser?Leu?Phe?Gln?Thr

195 200 205

Gly?Asp?Ile?Lys?Phe?Phe?Tyr?Thr?Ile?Ser?Arg?Phe?Ser?His?Pro?Leu

210 215 220

Gly?Leu?Ser?Tyr?Ser?Thr?Lys?Glu?Lys?Glu?Ala?Ile?Val?Arg?Leu?Ala

225 230 235 240

Gln?Arg?Tyr?Gln?Val?Tyr?Ile?Leu?Glu?Asp?Asp?Tyr?Leu?Gly?Asp?Phe

245 250 255

Val?Lys?Leu?Lys?Glu?Pro?Pro?Ile?His?Tyr?Tyr?Asp?Thr?His?His?Arg

260 265 270

Ile?Ile?Tyr?Leu?Lys?Ser?Phe?Ser?Met?Ser?Val?Phe?Pro?Ala?Leu?Arg

275 280 285

Ile?Gly?Ala?Leu?Val?Leu?Pro?Ser?Gly?Leu?Lys?Pro?His?Phe?Leu?Thr

290 295 300

Gln?Lys?Ser?Leu?Ile?Asp?Leu?Asp?Thr?Asn?Leu?Leu?Met?Gln?Lys?Ala

305 310 315 320

Leu?Ala?Leu?Tyr?Leu?Glu?Asn?Gly?Met?Phe?Gln?Lys?Asn?Leu?Arg?Phe

325 330 335

Ile?Lys?Arg?Tyr?Leu?Lys?Gln?Arg?Glu?Arg?Gln?Leu?Ala?Leu?Phe?Leu

340 345 350

Lys?Gln?Asn?Cys?Pro?Asp?Ile?His?Tyr?Gln?Leu?Thr?Pro?Thr?His?Leu

355 360 365

Val?Ile?Asp?Tyr?Thr?Thr?Ser?Asp?Ser?Tyr?Arg?Asn?Phe?Thr?Leu?Asp

370 375 380

Lys?Ser?Asp?Arg?Ile?Ile?Ile?Thr?Gly?Lys?Lys?Arg?Tyr?Leu?Ser?Ile

385 390 395 400

Thr?Ile?Asn?Gln?Gln?Ile?Gln?Ser?Lys?Leu?Asn?Ser?Leu?Ile?Lys?Asn

405 410 415

Thr?Cys?Gly?Lys?Ser?Asn

420

Claims (19)

1. the peptide that is used for the treatment of or diagnoses, it is by the operon coding that comprises any nucleotide sequence from streptococcus pyogenes as follows: SEQ ID NO.1,3,5,7,9,11,13,15,17,19,21,23,27,29,31,33,35,37,39,41,43,45,47,49,51,53,55,57,59 and 61, or the associated molecule of at least 40% sequence similarity or identity or its function fragment are arranged on peptide level or nucleotide level in the gram positive bacterium.

2. according to the peptide of claim 1, wherein said sequence similarity or identity are at least 60%.

3. according to the peptide of claim 1 or 2, wherein said sequence similarity or identity are at least 90%.

4. according to the peptide of claim 1, it comprises aminoacid sequence as follows: SEQ ID NO.2,4,6,8,10,12,14,16,18,20,22,24,28,30,32,34,36,38,40,42,44,46,48,50,52,54,56,58,60 or 62.

5. the coding that is used for the treatment of or diagnoses is according to the polynucleotide of the peptide of any aforementioned claim.

6. host, it is expressed according to each peptide of claim 1 to 4 through transforming.

7. attenuated microorganisms, it comprises and destroys the sudden change that any nucleotide sequence of definition is expressed in the claim 1.

8. according to the microorganism of claim 7, wherein sudden change is to insert inactivation or genetically deficient.

9. according to the microorganism of claim 7 or 8, wherein said microorganism is the A group streptococcus.

10. according to each microorganism in the claim 7 to 9, it is used for the treatment of or diagnoses.

11. according to each microorganism in the claim 7 to 10, it comprises sudden change in another nucleotide sequence.

12. according to each microorganism in the claim 7 to 11, it comprises heterologous antigen, therapeutic peptide or nucleic acid.

13. a vaccine, it comprises according to each peptide in the claim 1 to 4, or its phraseology.

14. a vaccine, it comprises according to each microorganism in the claim 7 to 12.

15. according to the purposes of each product in the claim 1 to 12, it is used to produce and is used for the treatment of or the medicine of the illness that prevention is relevant with suis or gram positive bacteria infection.

16. according to the purposes of claim 15, wherein said illness is a pneumonia.

17. according to the purposes of claim 15 or 16, it is used for veterinary treatment.

18. an antibody, it is at producing according to each peptide in the claim 1 to 4.

19. according to the purposes of each product in the claim 1 to 12, it is used to identify the shaker test of antimicrobial agents.