CN1800056A - Dye decolored degradation treatment method for dye-containing waste water - Google Patents
Dye decolored degradation treatment method for dye-containing waste water Download PDFInfo
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- CN1800056A CN1800056A CN 200510135000 CN200510135000A CN1800056A CN 1800056 A CN1800056 A CN 1800056A CN 200510135000 CN200510135000 CN 200510135000 CN 200510135000 A CN200510135000 A CN 200510135000A CN 1800056 A CN1800056 A CN 1800056A
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Abstract
The invention relates to a method of removing dye from waste water and decolorized degradation of dye molecule by biotechnology, belonging to environment protection technology field. Concrete processes are as follows: put fungi Penicillium sp.P-1 into dye water to adsorb dye for 2~5h, put said fungi with dye molecules into culture fluid and add bacteria Aeromonas sp.M10, etc, to said culture fluid to carry on decolorized degradation to dye molecules adsorbed on bacterial filaments. Two-type special microbe degrades multiple dye or other organic toxic molecules rapidly through co-metabolic reaction, co metabolism, etc, which could decrease the treatment cost of toxic material, avoid the danger of non-degradable organic dye molecules entering natural water body and accumulating and is characterized by appreciable economic benefits, environmental benefits and social benefits.
Description
Technical field
The present invention relates to a kind of treatment process of waste water containing dye, particularly a kind of biotechnology that adopts belongs to environmental protection technical field to the removal of dyestuff in the waste water containing dye and the treatment process of dye molecule decolored degradation.
Background technology
Because the diversity of dyestuff and printing and dyeing enterprise production kind and irregular property cause factory effluent character extremely unstable, the dyestuff kind changes frequent and is difficult to degraded in the waste water.Microorganism during conventional materialization is handled is difficult to the dye molecule of continuous variation is effectively degraded, even thereby the dye molecule of trace still is difficult to degraded after entering natural water body accumulate in environment, water ecological setting is caused huge infringement.Various dye molecules can be decomposed into small-molecule substance when the wet oxidation technology is handled waste water containing dye in the method commonly used at present and reach innoxious, but this technology needs wastewater temperature is risen to very high high temperature, thisly handles the technology cost of contained dye molecule degraded too high to whole waste water.
Adopt biologic treating technique, can carry out adsorption bleaching to multiple dyestuff in the waste water from dyestuff with fungies such as Penicillium notatums, as: " Penicillium notatum is to the adsorption of Reactive Brilliant Blue KN-R " [" using and the environmental organism journal " 2003,9 (2) .-179-182] in the literary composition, reported that Penicillium notatum (Penicillium X5) is to the adsorbing result of study of Reactive Brilliant Blue KN-R, but be adsorbed on dyestuff on the mycelia not by decolored degradation, still be present in the environment for objectionable impurities; Publication number is in the Chinese invention patent " saccharomycete and application thereof " of CN1654631A, provide a saccharomycete the decolouring liquid nutrient medium in to dye decolored method, dyestuff that nonnutritive thing is existed or dyeing waste water etc. then can't decolour, and add nutrition in waste water processing cost is increased.
Summary of the invention
The objective of the invention is to overcome the deficiency that prior art exists, provide a kind of microorganism to remove dye molecule in the waste water containing dye fast, the treatment process of its quick decolored degradation.
For achieving the above object, the technical solution used in the present invention is: is that 5～10% ratio is put in the dyestuff with Penicillium notatum P-1 (Penicillium sp.P-1) with mass percent, behind absorption 2～5h, the mycelia that is adsorbed with dye molecule is put back in the nutrient solution, add Aeromonas bacterium M10 bacterial strain (Aeromonas sp.M10) again, enterobacter bacterium L-1 (Enterobacter sp.L-1), a kind of among the pseudomonas putida L-2 (Pseudomonas putida L-2) or their mixing, the dye molecule that is adsorbed on the mycelia is carried out the decolored degradation processing, and the time is 5～96h.
The medium component of described Penicillium notatum P-1 is: the potato that contains 100g in every liter of nutrient solution cooks liquid, 5g glucose, and the 2g ammonium salt, the pH nature, solvent is a water; Training method is the 150rpm shaking culture, and culture temperature is 28 ℃, and incubation time is 36～48h.
Because selected fungi of technical solution of the present invention and bacterium can be to reactive dyestuffs, substantive dyestuff, weak acid dye, dispersed dye etc. are multiple dye adsorption, the special microorganism of 2 classes passes through cometabolism, interaction energies such as common metabolism are fast with multiple dyestuff or other organic poison molecule decolored degradation, make the adsorptive power of mycelia obtain regeneration simultaneously, therefore, can reduce the processing cost of objectionable impurities greatly, the dye molecule of having avoided belonging to hardly degraded organic substance enters natural water body and the danger that accumulates in environment, thereby produces tangible economic benefit, environmental benefit and social benefit.
The present invention can remove fast under field conditions (factors) that multiple dye molecule makes waste water decoloring in the waste water, and the decolored degradation in the little reactor of artificial control of the dye molecule after fungi absorption concentrates reaches innoxious, is used for the processing of multiple waste water containing dye.
Embodiment
Embodiment one:
The Penicillium notatum P-1 that is incubated at the test tube slant is made spore suspension to be inoculated in liquid nutrient medium (potato that contains 100g in every liter of nutrient solution cooks liquid, 5g glucose, the 2g ammonium tartrate, pH nature, solvent is a water), be under 28 ℃ the condition in temperature, 150rpm shaking culture 48h, from nutrient solution (keeping stand-by), take out mycelia and contain dyestuff or other organic poison waste water with 10% (w/v) ratio input, vibration or stirring 5h, waste water can adopt other method to enter next step processing.
The Penicillium notatum P-1 mycelia that is adsorbed with dye molecule that to take out from waste water is put back in the original fluid, adds enterobacter bacterium L-1, pseudomonas putida L-2 again, handles 5～96h.In this process, the synergy of Penicillium notatum P-1 and mixed bacterium can make the degraded of dyestuff or other organic poison to being adsorbed on the quick decolored degradation of dye molecule on the mycelia.
Present embodiment is selected brilliant blue KN-R, dark blue M-2GE, the blue SD-GSL of dispersible cationic, active deep blue B-2GLN, bright blue KN-6, yellow M-3RE, direct sun-proof strain 4BS, reactive red 3BE, waste water from dyestuff such as reactive scarlet B-3G, gorgeous purple KN-B, after handling as stated above, chroma removal rate reaches more than 90%, light yellow KM-7G, orange KN-5R, weak lime gs N, Congo red chroma removal rate are reached 70～90%, 1000 times black purple dye bath chroma in waste water clearances are reached about 70%.
Embodiment two:
In the brilliant blue KN-R of 100mg/L, reactive red 3BE, reactive scarlet B-3G, gorgeous purple KN-B dyestuff, add Penicillium notatum P-1 mycelia respectively, 100rpm vibration absorption 5h, get the 0D value of the dyestuff supernatant liquor usefulness spectrophotometric determination dyestuff maximum absorption wave strong point after fungi is handled, with distilled water is contrast, measure percent of decolourization, with the clearance of expression to dyestuff in the dyestuff.Clearance (%)=(A-B)/A * 100 (the OD value before A-water treatment, the OD value of B-dyestuff after fungi is handled), above-mentioned dyestuff chroma removal rate reaches more than 90%.
The mycelia that is adsorbed with dyestuff is put into original fluid, add Aeromonas bacterium M10 bacterial strain, enterobacter bacterium L-1, pseudomonas putida L-2 mixt bacteria again, be under 30～35 ℃ the condition in temperature, the processing of leaving standstill through 5～96h, by effects such as the cometabolism of above-mentioned two class peculiar microorganisms, common metabolism, can see that the color restoration on the hypha,hyphae is the white before the absorbing dye, the complete decolored degradation of dye molecule.
Embodiment three:
To colourity is to add Penicillium notatum P-1 mycelia in the highest dye bath waste water of 1000 times silk printing and dyeing mill dye strength, 100rpm vibration absorption 5h, get the OD value of the dye bath wastewater supernatant fluid usefulness spectrophotometric determination dyestuff maximum absorption wave strong point after fungi is handled, with distilled water is contrast, measure percent of decolourization, with the clearance of expression to dyestuff in the waste water from dyestuff.Clearance (%)=(A-B)/A * 100 (the OD value before A-wastewater treatment, the OD value of B-waste water after fungi is handled).Dye bath chroma in waste water clearance can reach about 70%.
The mycelia that is adsorbed with dyestuff is put into original fluid, add Aeromonas bacterium M10 bacterial strain, enterobacter bacterium L-1, pseudomonas putida L-2 mixt bacteria again, be under 30～35 ℃ the condition in temperature, the processing of leaving standstill through 48～72h, by effects such as the cometabolism of two class peculiar microorganisms, common metabolism, can see that the color restoration on the hypha,hyphae is that the white before the absorbing dye is dye molecule and decolours fully.Technical solutions according to the invention are applicable to the processing that contains other difficult degradation organic poison waste water, and effect is obvious.
Claims (2)
1. the removal of dyestuff and the treatment process of dye molecule decolored degradation in the waste water containing dye, it is characterized in that: is that 5～10% ratio is put in the waste water that contains dyestuff with Penicillium notatum P-1 (Penicillium sp.P-1) with mass percent, behind absorption 2～5h, the mycelia that is adsorbed with dye molecule is put back in the nutrient solution, add Aeromonas bacterium M10 bacterial strain (Aeromonas sp.M10) again, enterobacter bacterium L-1 (Enterobacter sp.L-1), among the pseudomonas putida L-2 (Pseudomonas putida L-2) one or more, the dye molecule that is adsorbed on the mycelia is carried out the decolored degradation processing, and the time is 5～96h.
2. the treatment process of the removal of dyestuff and dye molecule decolored degradation in a kind of waste water containing dye according to claim 1, it is characterized in that: the medium component of described Penicillium notatum P-1 is: the potato that contains 100g in every liter of nutrient solution cooks liquid, 5g glucose, the 2g ammonium salt, the pH nature, solvent is a water; Training method is the 150rpm shaking culture, and culture temperature is 28 ℃, and incubation time is 36～48h.
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| CN 200510135000 CN1800056A (en) | 2005-12-29 | 2005-12-29 | Dye decolored degradation treatment method for dye-containing waste water |
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| CN 200510135000 CN1800056A (en) | 2005-12-29 | 2005-12-29 | Dye decolored degradation treatment method for dye-containing waste water |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948778A (en) * | 2010-08-30 | 2011-01-19 | 中国农业大学 | Bacillus megaterium and application thereof to printing and dyeing wastewater treatment |
| CN103614300A (en) * | 2013-11-08 | 2014-03-05 | 浙江大学 | Acrogenospora sphaerocephala and application thereof |
| CN103896408A (en) * | 2014-02-13 | 2014-07-02 | 温州科技职业学院 | Method for efficiently degrading azo dye in dye wastewater by using bacteria |
| CN103910435A (en) * | 2014-01-14 | 2014-07-09 | 暨南大学 | Use of Lysinibacillus sp. FS1 in dye degradation |
| CN103937697A (en) * | 2014-01-14 | 2014-07-23 | 暨南大学 | Bacterial strain for degrading dye with high efficiency |
| CN104289196A (en) * | 2014-10-16 | 2015-01-21 | 黄淮学院 | Method for modifying tea oil shell dye adsorbent |
| CN104560822A (en) * | 2014-12-31 | 2015-04-29 | 黑龙江大学 | Psychrotolerant bacterium with high flocculation activity and with decolorization on methylene blue |
-
2005
- 2005-12-29 CN CN 200510135000 patent/CN1800056A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948778A (en) * | 2010-08-30 | 2011-01-19 | 中国农业大学 | Bacillus megaterium and application thereof to printing and dyeing wastewater treatment |
| CN103614300A (en) * | 2013-11-08 | 2014-03-05 | 浙江大学 | Acrogenospora sphaerocephala and application thereof |
| CN103910435A (en) * | 2014-01-14 | 2014-07-09 | 暨南大学 | Use of Lysinibacillus sp. FS1 in dye degradation |
| CN103937697A (en) * | 2014-01-14 | 2014-07-23 | 暨南大学 | Bacterial strain for degrading dye with high efficiency |
| CN103896408A (en) * | 2014-02-13 | 2014-07-02 | 温州科技职业学院 | Method for efficiently degrading azo dye in dye wastewater by using bacteria |
| CN104289196A (en) * | 2014-10-16 | 2015-01-21 | 黄淮学院 | Method for modifying tea oil shell dye adsorbent |
| CN104560822A (en) * | 2014-12-31 | 2015-04-29 | 黑龙江大学 | Psychrotolerant bacterium with high flocculation activity and with decolorization on methylene blue |
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