CN1795767A - Oxidation resistant composition - Google Patents
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- CN1795767A CN1795767A CN 200410102663 CN200410102663A CN1795767A CN 1795767 A CN1795767 A CN 1795767A CN 200410102663 CN200410102663 CN 200410102663 CN 200410102663 A CN200410102663 A CN 200410102663A CN 1795767 A CN1795767 A CN 1795767A
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Abstract
An antioxidizing composition for food, cosmetics and medicine in order to suppress the generation of active oxygen chosen from O2, H2O2 and HOCl and decrease the content of said active oxygen features that is contains theanine.
Description
Technical field
The present invention is with to contain the antioxidant composition that theanine is a feature (the following composition that abbreviates as sometimes) relevant.
Background technology
Learn in recent years active oxygen in vivo organism defence system and cell in signal system serve as important process, but then biological component is caused various dysoxidations and becomes the reason of aging and disease.
With regard to active oxygen, can give an example as peroxide (O2
-), hydroxy radical (HO), hydroperoxidation free radical (HOO), alkoxy free group (LO), alkane peroxylradicals (LOO), nitric oxide radical types such as (NO), and hydrogen peroxide (H
2O
2), singlet state oxygen (
1O
2, singletoxygen), peroxidating nitrate (ONOO
-), MDA (LOOH), inferior chlorous acid (HOCl), ozone (O
3) wait the non-free radical class.Wherein, superoxides (O
2 -), hydroxy radical (HO), hydrogen peroxide (H
2O
2), and singlet state oxygen (
1O
2) be called the active oxygen of narrow sense.
As for of the influence of these active oxygens, can give an example and untie and the tissue damage that causes because of linksystem generation free radical etc. as the part of DNA spiral to organism.Its result is known to cause allergic reactions such as bronchial allergy, cause inflammation because of discharging histamine, miocardial infarction, the deterioration of ischemic heart diseases such as angina pectoris and oxyhepatitis, liver disorder diseases such as fatty liver, bring out the cerebrovascular disorders such as cerebral infarction that information between cranial nerve cell passes on the active oxygen that the too much stimulation of various acceptors produced for important glutamic acid to cause, the A Zihai type dementia of writing from memory, Parkinson's and the dementia that causes because of brain tissue impairment, and premature's retinopathy, pulmonary emphysema, diabetes, gout, cutaneum carcinoma, toxaemias of pregnancy, glomerulus body ephritis, cataract, retina iron rust disease, spot, one of disease reasons such as wrinkle.
In addition, active oxygen to the influence of organism not only as above-mentioned often becomes aspect food and causes rotten or deterioration or quicken rotten or deterioration is a matter of public knowledge.
Therefore, suppress oxygen production or reduce oxygen production, in treatment or prevent these diseases and inhibition or prevention food cause aspect rotten or the deterioration very important at organism or food.Therefore, can suppress in vivo or food produces the material of active oxygen for having, or have and can reduce in vivo or food produces the material (below be sometimes referred to as polyphenoils) of the effect (being antioxidation) of active oxygen, extensively explore.
Polyphenoils, can give an example as fat-soluble tocopherol (vitamin E), the carotenoid (carotenoid) that is derived from natural goods, water miscible ascorbic acid (vitamin C) etc., and the fourth hydroxy-methylbenzene (BHT) of synthetic compound, fourth hydroxyanilines (BHA) etc.
Moreover it is that purpose is tried out superoxide dismutase (SOD) to reduce active oxygen that report is arranged.
In addition, when also having report that amino acid is used as polyphenoils, performance prevents the effect (as reference patent documentation 1) of skin aging and various diseases.
In addition, recently for trying to achieve sufficient drug effect and security, and the material that examination obtains having antioxidation by the crude drug extract also a lot (as reference patent documentation 2～14), these all utilize the tool antioxidation material that is derived from crude drug or plant.
" patent documentation 1 " international specification that discloses No. 94/14755
" patent documentation 2 " Japan Patent spy opens clear 60-224629 communique
" patent documentation 3 " Japan Patent spy opens clear 61-24522 communique
" patent documentation 4 " Japanese patent laid-open 2-193930 communique
" patent documentation 5 " Japanese patent laid-open 2-243632 communique
" patent documentation 6 " Japanese patent laid-open 2-264727 communique
" patent documentation 7 " Japanese patent laid-open 3-153629 communique
" patent documentation 8 " Japanese patent laid-open 3-221587 communique
" patent documentation 9 " Japan specially Zhu, a state in the Zhou Dynasty spy opens flat 4-69343 communique
" patent documentation 10 " Japanese patent laid-open 4-202138 communique
" patent documentation 11 " Japanese patent laid-open 4-247010 communique
" patent documentation 12 " Japanese patent laid-open 10-226787 communique
" patent documentation 13 " Japan Patent spy opens the 2000-129256 communique
" patent documentation 14 " Japan Patent spy opens the 2002-308767 communique
Summary of the invention
The problem that invention institute desire solves
But the active oxygen minimizing effect of above-mentioned polyphenoils is insufficient, and BHT and BHA have the doubt of carcinogenicity, so be difficult to all think that practicality is arranged.
And because of SOD is a protein, not only it obtains difficulty, and the problem that is digested when having oral throwing to give, even if adopt injection and throw when giving for head it off changes, the half-life in its blood is short, so can't satisfy as polyphenoils.
Moreover above-mentioned amino acid is not naturally occurring amino acid, is not used as food or food additives use by approval, so can not be used in former food.
Therefore, the object of the present invention is to provide and to use as food, cosmetics and pharmaceuticals, can suppress the generation of active oxygen, and can reduce a kind of antioxidant composition of the active oxygen that is taken place.
The means of dealing with problems
Also promptly, the present invention is
(1) contains the antioxidant composition of theanine component;
(2) above-mentioned (1) described antioxidant composition can suppress to be selected from O
2 -, H
2O
2Reach the generation that HOCl constitutes the active oxygen in the group, maybe can reduce this active o content;
(3) above-mentioned (1) or (2) described antioxidant composition are food or pharmaceuticals; And
(4) above-mentioned (3) described antioxidant composition is any of lozenge or capsule.
The invention effect
Therefore, according to antioxidant composition of the present invention, can reach the generation that can suppress active oxygen, and can reduce the effect of the active oxygen that is taken place.Moreover antioxidant composition of the present invention can use as food, cosmetics and pharmaceuticals.
Brief Description Of Drawings
Fig. 1 shows that theanine is to superoxides (O
2 -) antioxidation.
Fig. 2 shows that theanine is to superoxides (O
2 -) antioxidation.
Fig. 3 shows the antioxidation of theanine to inferior chlorous acid (HOCl).
Fig. 4 shows that theanine is to hydrogen peroxide (H
2O
2) antioxidation.
The specific embodiment
Antioxidant composition of the present invention is a big feature to contain theanine.
In addition, for the present invention, so-called anti-oxidant generation or the minimizing active oxygen that promptly refers to suppress active oxygen.
With regard to active oxygen, can give an example as superoxides (O
2 -), hydroxy radical (HO), hydroperoxidation free radical (HOO), alkoxy free group (LO), alkane peroxylradicals (LOO), nitric oxide radical types such as (NO), and hydrogen peroxide (H
2O
2), singlet state oxygen (
1O
2), peroxidating nitrate (ONOO
-), MDA (LOOH), inferior chlorous acid (HOCl), ozone (O
3) wait the non-free radical class.Wherein, superoxides (O
2 -), hydroxy radical (HO), hydrogen peroxide (H
2O
2), and singlet state oxygen (
1O
2) be called the active oxygen of narrow sense.
Whether theanine shows antioxidation, can be as using peroxide valence state determination methods such as iodimetry, thiocyanate-ferric, measure the analyte determination method of carbonyl valency, TBA valency, volatile materials etc., according to using DO determination meter or Wa Shi (Warburg) meter or according to the method for gravimetric detemination zmount of oxygen consumption, ultraviolet radiation absorption divides that folding, infrared ray absorbing divide folding, electron cyclotron resonace to absorb, fluorescence divides folding, atomic weak light divides physico-chemical processes such as folding, and the method for use living things system etc. is assessed.
Be used in theanine of the present invention, be the derivative (γ-glutaric acid buserelin) of glutamic acid, be rich in aminoacid ingredient in tealeaves at nature.Be used in the method for making of theanine of the present invention, as method by tea extraction, methodology of organic synthesis is (with reference to Chem.Pharm.Bull., 19 (7): 1301-1307 (1971)), make glutaminase act on glutamic acid and ethamine mixture and the method (with reference to the special fair 7-55154 communique of Japan Patent) of theanine, to contain the medium culture tea cell mass of ethamine, in increasing tea cultured cell group, manage to promote to cultivate the method (with reference to Japanese patent laid-open 5-123166 communique) of the propagation of tea cell mass under the theanine accumulation state, and as special fair 7-55154 communique and the special method of opening as suggested in the flat 5-123166 communique 1-ethanamine derivatives such as ethamine being substituted by ethylamine hydrochloride and must the L-theanine etc., any method all can.In addition, above-mentioned tealeaves can be given an example as green tea, oolong, black tea etc.
Theanine no matter L-theanine, D-theanine, DL-theanine all can use, and wherein, the L-body approved to use and be food additives, and utilizes easily economically, so better with the L-body in the present invention.Again, the form of the theanine that uses all can as various forms such as highly finished product, thick highly finished product, extraction essences.Use commercially available product (solar tea propylhomoserin (suntheanine, registration mark), sun Chemical Corporation's system) also can again.
Theanine content in composition of the present invention is not particularly limited, can be according to desired and suitable adjustment.When for example considering the desirable effect of performance the present invention, the theanine content that is fit in the preferred composition is 0.01～99.9 weight %, and more preferably 0.1～99 weight %.
The detection method of theanine content is not particularly limited in the composition of the present invention, can give an example and detect quantitative methods with fluorescence detector again, or separate the back with 210 mum wavelengths detection quantitative methods etc. with efficient liquid-phase tube column chromatography (HPLC) with the ODS tubing string as separating with efficient liquid-phase tube column chromatography (HPLC) with the ODS tubing string after the materialization of deriving with the preposition tubing string of adjacent phthalaldehyde (OPA, Orthophthal aldehyde).
Composition of the present invention by the viewpoint of strong antioxidant action more, preferably contains active oxygen scavenger jointly.Oxygen scavenging activity agent content in the composition of the present invention is not particularly limited, and can comply with desired and suitable adjustment.When for example considering the desirable effect of performance the present invention, the oxygen scavenging activity agent content that is fit in the composition is preferably 0.01～99.9 weight %, and more preferably 0.1～99 weight %.So-called active oxygen scavenger, promptly refer to have the material of the character of removing superoxides, hydrogen peroxide, hydroxy radical, singlet state oxygen isoreactivity oxygen, can give an example as catechuic acid (catechin) class, tannin class, anthocyanin class, flavonoid class, saponin class and these various relevant composition.These can separately or mix more than 2 kinds and use.The great majority of these active oxygen scavengers, confirmation form is unique faint scavenging action or an antioxidation.
The catechu acids is not particularly limited, and can give an example as catechuic acid, catechuic acid gallic acid ester, epicatechin, epicatechin gallate, table gallocatechin, table gallocatechin gallic acid ester, gallocatechin, gallocatechin gallic acid ester etc.
The tannin class can greatly Wei hydrolysis-type tannin and condensed type tannin.So-called hydrolysis-type tannin promptly refers to produce because of hydrolysis the tannin of gallic acid or ellagic acid, but is not particularly limited, and can give an example as Gallate tannin, ellagic acid tannin, tealeaves gallin, chlorogenic acid, neochlorogenic acid etc.So-called condensed type tannin promptly refers to have flavones-tannin that the catechu acids polycondensation of 3-ol skeleton forms, but be not particularly limited, can give an example as former anthocyanin class such as an anthocyanidin, delphinidins.
Anthocyanin class is not particularly limited; can give an example as non-acyl group anthocyanin; the organic acyl group anthocyanin of the aliphatic of acyl group anthocyanin, monoacylation anthocyanin, polyamides base anthocyanin, the anthocyanin that the common pigment (Co-pigment) of compound materialization anthocyanin is changed, metal anthocyanin, hordenine (hordemine), red wine pigment etc.
Flavonoid class is not particularly limited, and can give an example as flavones, flavones-3-alcohol, flavanones, flavanols, isoflavones etc.
The saponin class can not be greatly three terpenoid type saponin and steroids type saponin.Three terpenoid type saponin are not particularly limited, and can give an example as contained oleanane (oleanane) type saponin such as balloonflower root, horse chestnut, cyclamens contained big horse tree alkane (damarane) the type saponin of ginseng for medicinal use.Steroids type saponin is not particularly limited, and can give an example as the contained dioscin of mountain Bi (dioscin), the contained wind-weed saponin of wind-weed A-III, the contained ophiopogonin of the tuber of dwarf lilyturf (ophiopogonin A), the contained digitonin of digitalis etc.
These active oxygen scavengers are contained a lot of in crude drug, medicine among the people, medicinal herbs, vegetable food (as beans, five cereals, vegetables, fruit etc.), animal wait with bait (herbage, rice bran etc.), therefore can use and wait the extract that material, raw material extract with known method thus or directly use these materials, raw material also can.These materials, raw material can separately or mix more than 2 kinds and use.
Crude drug is not particularly limited, and can give an example as valerian (valerian), Radix Angelicae Sinensis, Chinese herbaceous peony, tree peony, korean ginseng, balloonflower root (root), Radix Glycyrrhizae (rhizome), polygala root (senega) (root), Quillaia saponaria (bark), digitalis (leaf), mountain Bi (rhizome), sarsa (sarsa) (root), the wind-weed (root of Hanasuge) etc.Its form also is not particularly limited, and can give an example as extract, dry thing etc.
Medicine among the people is not particularly limited, and can give an example as root tuber of aromatic turmeric, sweet tea, fig, chamomile, west safflower, genseng, the seed of Oriental arborvitae etc.
Medicinal herbs are not particularly limited, and can give an example as anise, carrot seed, cloves, coriander, cypress, Chinese cassia tree, needle juniper, ginger, sweet orange, the needle pine, sweet basil (Basil), Pogostemon cablin, bigarabe, fennel, black pepper, bay tree, peppermint, bergamot, tangerine, myrrh, lemon grass, rosemary,, grape fruit, cdear (arborvitae), lemongrass (cus-cus), Salvia japonica, thyme, Australia tea tree, the violet leaf, vanilla, hyssop, outstanding markon, lime, lemon, the Yilan, cardamom, fragrant purple perilla (Clary sage), jasmine, fish pelargonium, chamomile, Bulgaria rose, rose, frankincense, lavender, chamomile, fish pelargonium, sandalwood, flores aurantii, the beauty cherry, Petitgrain, training ground thatch (cus-cus), marjoram (Marjoram), balm, rosewood, hypericum perforatum, St. john's wort, Kava Kava etc.Its form also is not particularly limited, and can give an example as extracting essence, essential oil, herbal tea etc.
Vegetable food is not particularly limited, and can give an example as soya bean, red bean, French beans, spinach, beet, Chinese yam, edible lily, Leek dish, green onion, Chinese onion, garlic, shaddock, Momordica grosvenori, grape, carrot, tomato, onion, luxuriant pomegranate, Cranberry etc.
Animal is not particularly limited with bait, can give an example as cereals such as herbage, maize, Chinese sorghum, wheat classes distillers' grains classes such as wheat bran, rice bran class, vegetalitas oil meal class etc.
Moreover composition of the present invention can contain amino acid, vitamin, mineral matter jointly, reach permissible material, raw material on other food.These all can separately or mix more than 2 kinds and use.
Amino acid is not particularly limited, and can give an example as glutamic acid, glutamine, inosinicacid, alanine, arginine, asparatate, threonine, serine, GABA, taurine, thiolaurine, hypotaurine etc.
Vitamin is not particularly limited, and can give an example as vitamin A, vitamin B1, vitamin B2, vitamin B6, cobalamin, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, niacin, lipoic acid (lipoic acid), pantothenic acid, biotin, ubiquinone (ubiquinone), prostate element and these derivative etc.
Mineral matter is not particularly limited, and can give an example as calcium, iron, magnesium, copper, zinc, selenium, potassium etc.
Permissible material on other food, raw material, can give an example as aloe, bee milk, take off melanin, placental hormone, propolis, isoflavones, soybean lecithin, egg yolk lecithin, egg oil, chondroitin, cocoa mud, collagen, vinegar, green alga, the spiral blue-green algae, ginkgo leaf, green tea, bark of eucommia tea, Chinese wolfberry tea, oolong tea, mulberry leaf, sweet tea, Ba Naba (Banaba, banaba) tea, unrighted acid, carbohydrate such as sugar alcohol and compound sugar, than mushrooms such as Fei Duosi bacterium and red colouring agent for food, also used as a Chinese medicine, straw mushroom, Ji's young pilose antler, glossy ganoderma, mushroom classes such as dance young pilose antler, blueberry, plum, grape, olive, plum, fruits such as citrus, peanut, almond, flax, peppers etc. are planted subclass, pimento, capsicum, long, rectangular bag, pumpkin, cucumber, carrot, burdock, Egyptian wild flax baby, garlic, purple perilla, horseradish, tomato, Chinese onion, leaf vegetables, taro, greengroceries such as beans, sea grass classes such as edible seaweed, fish and shellfish, beastly bird whale meat, cereals etc. are directly used or its extract, dry thing, thick highly finished product, highly finished product, processed goods, brewing material etc.
In addition, composition of the present invention, by suitable viewpoint used in everyday, better with food or pharmaceuticals.
In food of the present invention, be not limited only to contain the food of theanine, also comprise the food additives that contains theanine.
Be contained in food of the present invention, can give an example, reach liquid foods such as cold drink, mineral water, hobby beverage, alcoholic beverage as solid foods such as dried foods, supplementary foods.Be not particularly limited the goods like a white silk of to give an example, soybean processing product, flavoring, Mu Si, jelly, Ice, maltose, chocolate, chewing gum, soda cracker, cake, bread etc. as for solid food.Liquid food is not particularly limited again, can give an example as teas such as green tea, oolong tea, black tea, Herb Teas inspissated juice, concentrated original fruit juice, pure juice, blended fruit juice, pulp juice, fruit drink, fruit and vegetable juice, vegetable juice, soda, cold drink, newborn class beverage, Janpanese sake, beer, red wine, cocktail, liquor, the whiskey etc. gone back.
And, be contained in pharmaceuticals of the present invention, promptly be not particularly limited as long as contain theanine.For example, its form with solution, suspension, powder, solid shaping thing all can, and its formulation can be given an example and be paid agent, suppository, inhalant etc. as lozenge, capsule, powder, granule, oral solutions, injection, subsides.
The method for making of composition of the present invention is not particularly limited, the method for making that theanine and other raw material are mixed with powdery or theanine and other raw material be dissolved in the method for making that becomes mixed solution in the solvent or for example with this mixed solution freeze drying or spray-dired method for making etc., the method for making of applicable normal food or pharmaceuticals.
For example when food, existing food is cooperated theanine with conventional method, make in the food after the manufacturing theanine content in desired scope, to make food of the present invention.And for example for pharmaceuticals, also identical with manufacturing food, with conventional method and be fit to organic or inorganic carrier that oral throwing gives, excipient, binder, stabilization agent etc. and cooperate, make theanine content in desired scope, theanine to make pharmaceuticals of the present invention.
The composition of the present invention that as above makes is to active oxygen, especially O
2 -, H
2O
2Or HOCl, have antioxidation.
For obtaining the desirable effect of the present invention, the throwing amount of giving of composition of the present invention if calculate with theanine, is good with 1～1000mg/ day then usually, and 5～500mg/ day is better.But because of a guy's difference (symptom kind and degree, age etc.),, and be to obtain the desirable effect of the present invention, can suitably set each one concrete throwing amount of giving so the throwing amount of giving of the present invention does not limit as above-mentioned scope.
The throwing method of giving of composition of the present invention is not particularly limited, but with oral throwing give, vein is thrown and to be given, rectum is thrown and given to preferably, and gives to more preferably with oral throwing, above-mentionedly wish in the scope as long as throw to give.
It is safe for theanine used in the present invention, for example in acute toxicity test in mice, do not have dead example when 5g/kg dosage is given in oral throwing, and its general state and body weight etc. is also no abnormal.Especially the L-theanine is known as the well tasting composition of tea, and the food additives that also can be used as the purposes of rendering palatable uses, and is also unrestricted to its addition on food hygiene law.And different with former medicine, theanine has no side effect fully, so safety can be provided and have the composition of imitating antioxidation according to the present invention.
Below, describe the present invention in detail with embodiment and test example, but the present invention is not subjected to the qualification of these embodiment and test example.
Production Example 1 is with ferment manufactured L-theanine
Under 30 ℃, 0.3M glutamic acid and 1.5M ethamine were reacted 22 hours in 0.05M borate buffer (Na284O7-NaOH, pH11), in the presence of the 0.3U glutaminase, obtain theanine 225nmol.Secondly, by the refining theanine of reactant liquor, reactant liquor is undertaken using Ethanol Treatment again after the chromatography by Dowex50 * 8 tubing strings and Dowex 1 * 2 tubing string (being chamber raised path between farm fields chemical industrial company system).
As for the affirmation of L-theanine, above-mentioned refining gained separate substance is confirmed that with amino-acid analyzer (Hitachi's corporate system) and filter paper chromatography method separate substance is identical with standard substance.Secondly, during with hydrochloric acid or glutaminase hydrolysis process separate substance, produce glutamic acid and ethamine with 1: 1 ratio.So, learnt that by the glutamine enzyme hydrolysis ethamine is in the γ position of glutamic acid bond by separate substance.In addition, the glutamic acid of hydrolysis generation is also confirmed as L-glutamic acid with glutamte dehydrogenase.By said procedure, can confirm that at last the gained compound is the L-theanine.This moment, gained L-theanine was 8.5g.
Production Example 2 is by tea extraction L-theanine
Tea (Camellia sinensis) leaf 10kg with the water hot extraction after, with extract by cationic ion-exchange resin (chamber raised path between farm fields chemical industrial company system, Dowex HCR W-2), with 1N NaOH stripping.Stripping uses RO film (day eastern electrician's corporate system, NTR 729HF) to concentrate its 15% ethanol stripping partly partly by activated carbon (two village chemical industrial company systems, close very much activated carbon SG) again.Secondly, refining with the tubing string chromatography, and crystallization again, get L-theanine 24.8g.
In addition, following embodiment uses solar tea propylhomoserin (suntheanine, registration mark) (sun Chemical Corporation's system) as theanine.
The appraisal procedure of antioxidation
The antioxidation of composition of the present invention is assessed by the chemiluminescence that luminescent substance takes place because of active oxygen with chemiluminescent analyzer (CLA-SP2, northeast electronic industry corporate system, Chemiluminescence Analyzer) mensuration.Detect wave-length coverage and be set at 400～870nm, resolution then in 270 and 870nm between the band wavelength set be 4nm.
Embodiment 1 theanine to superoxides (O
2 - ) antioxidation
Theanine is dissolved in the 50mM phosphate buffer (pH7.4), make its ultimate density become respectively 0,1,10 and 100mg/ml with the modulation sample.Each 200 μ l of sample thief put into the sample room of chemiluminescent analyzer (CLA-SP2, northeast electronic industry corporate system) respectively, are adding sample after 100 seconds, add 0.1mM lucigenin (Lucigenin) 500 μ l and be used as luminescent substance to the sample room.Secondly, adding sample after 150 seconds, 0.1mM xanthine 500 μ l and 0.004U xanthine oxidase 50 μ l are added in the sample room.The chemiluminescent result that measures shows as Fig. 1 in this sequence of operations.
Secondly, respectively get theanine 0,100,150,200 and 300mg, mix with 50mM phosphate buffer (pH7.4) 200 μ l respectively and be modulated into sample.Each sample is put into the sample room of chemiluminescent analyzer (CLA-SP2, northeast electronic industry corporate system) respectively, adding sample after 100 seconds, add 0.1mM lucigenin (Lucigenin) 500 μ l and to the sample room, be used as luminescent substance.Secondly, add sample after 150 seconds, adding 0.1mM xanthine 500 μ l and 0.004U xanthine oxidase 50 μ l to the sample room.Measure and add xanthine and the chemiluminescence of xanthine oxidase after 150 seconds, its result shows as Fig. 2.
As shown in Figure 1, when more not containing the chemical luminescent detecting value of the blank group of theanine and each processed group, its measured value reduces with theanine concentration, confirms the antioxidation of theanine to superoxides (O2-).Again as shown in Figure 2, its chemical luminescent detecting value showed than blank group minimizing (p＜0.05) when interpolation theanine 100mg was above, confirmed superoxides (O2
-) antioxidation.
Get theanine 0,10,50,100,150,200 and 300mg, mix with 50mM phosphate buffer (pH7.4) 200 μ l respectively and be modulated into sample.Each sample is put into the sample room of chemiluminescent analyzer (CLA-SP2, northeast electronic industry corporate system) respectively,, add 25 μ M luminols (Luminol), 500 μ l to the sample room, be used as luminescent substance adding sample after 100 seconds.Secondly, adding sample after 150 seconds, add % chlorous acid of 0.12 weight 100 μ l to the sample room.Measure and add time chemiluminescence of chlorous acid after 150 seconds, its result shows as Fig. 3.
Add the result of theanine, the chemical luminescent detecting value shows than blank group minimizing (p＜0.05), confirms that theanine is to inferior chlorous antioxidation.
Embodiment 3 theanine to hydrogen peroxide (H2O2
) antioxidation
Get theanine 0,200 and 300mg, mix with 50mM phosphate buffer (pH7.4) 200 μ l respectively and be modulated into sample.Each sample is put into the sample room of chemiluminescent analyzer (CLA-SP2, northeast electronic industry corporate system) respectively,, add 25 μ M luminols (Luminol), 500 μ l to the sample room, be used as luminescent substance adding sample after 100 seconds.Secondly, adding sample after 150 seconds, add 0.3 weight % hydrogen peroxide (H
2O
2) to the sample room.Measure and add the chemiluminescence of hydrogen peroxide after 150 seconds, its result shows as Fig. 4.
Add the result of theanine, the chemical luminescent detecting value shows than blank group minimizing (p＜0.05), confirms the antioxidation of theanine to hydrogen peroxide.
Embodiment 4 contains the manufacturing of theanine lozenge
Mix following raw material, and the granulation briquetting becomes every ingot 0.75g, make and contain theanine lozenge, be used as 1 example of antioxidant composition of the present invention.
The sugared 71.67 weight % (0.5375g) of frost
Cocoon honey (trehalose) 10.00 weight % (0.0750g)
Theanine 13.33 weight % (0.1000g)
Sucrose fatty ester 1.0 weight % (0.0075mg)
Spices (high sand perfume industry corporate system) 4.0 weight % (0.0300mg)
Add up to 100 weight % (0.7500g)
Theanine 10kg is dissolved in 10kg water with the modulation theanine solution.Secondly, be heated to 110 ℃, add theanine solution and water maltosemalt sugar again while particulate sugar 64kg is dissolved in water 20kg, and with temperature increase to 145 ℃.After stopping heating, add the tartaric acid 1kg and the spices 1kg (high sand perfume industry corporate system) that mix 50 weight %.Be cooled to 75～80 ℃, be shaped, make and contain the theanine candy, be used as 1 example of antioxidant composition of the present invention with shaping roller.In addition, measure the result of theanine content in the candy, every contains 1.2g, i.e. 89.6mg/g.
Embodiment 6 contains the manufacturing of theanine blueberry drink
Fructose glucose 12kg, blueberry inspissated juice 1kg, 1/5 transparent lemon juice 0.4kg, natrium citricum 0.05kg and theanine 0.1kg are added stirring and dissolving in the entry.Adjust pH with 50 weight % natrium citricums (crystallization) and be heated to 95 ℃ after 3.1, again perfuming 0.05kg (high sand perfume industry corporate system) and add water and make total amount become 100kg.Tinning makes every jar to be sterilization behind the 100g, cooling, makes to contain the theanine blueberry drink, is used as 1 example of antioxidant composition of the present invention.In addition, measure the result of theanine content in the blueberry drink, its content is 98.3mg/100g.
Embodiment 7 contains the manufacturing of theanine grape fruit beverage
Fructose Glucose Liquid 6kg, theanine 0.1kg, ferric pyrophosphate 0.06kg, the smart 0.01kg of placental hormone and 100% grape fruit fruit juice 30kg are added stirring and dissolving in the entry.Adjust pH with natrium citricum and be heated to 95 ℃ after 3.1, again perfuming 0.05kg (high sand perfume industry corporate system) and add water and make total amount become 100kg.Tinning makes every jar to be sterilization behind the 100g, cooling, makes to contain theanine grape fruit beverage, is used as 1 example of antioxidant composition of the present invention.In addition, measure the result of theanine content in the grape fruit beverage, its content is 96.4mg/100g.Load respectively, cool off with 100ml, make and contain theanine grape fruit beverage.
The possibility of utilizing on the industry
You Yu antioxidant composition safety of the present invention and have the Xiao antioxidation is so can be used as Food, cosmetics and pharmaceuticals use.
Claims (4)
1. antioxidant composition, it contains the theanine component.
2. antioxidant composition as claimed in claim 1, it can suppress to be selected from O
2 -, H
2O
2And the generation of the active oxygen of HOCl, and can reduce this active o content.
3. antioxidant composition as claimed in claim 1 or 2, it is food or pharmaceuticals.
4. antioxidant composition as claimed in claim 3, it is lozenge or capsule any.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410102663 CN1795767A (en) | 2004-12-24 | 2004-12-24 | Oxidation resistant composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410102663 CN1795767A (en) | 2004-12-24 | 2004-12-24 | Oxidation resistant composition |
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| CN1795767A true CN1795767A (en) | 2006-07-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200410102663 Pending CN1795767A (en) | 2004-12-24 | 2004-12-24 | Oxidation resistant composition |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103371216A (en) * | 2012-04-24 | 2013-10-30 | 西姆莱斯有限公司 | Metal-complexing aroma compounds for use in aroma stabilization |
-
2004
- 2004-12-24 CN CN 200410102663 patent/CN1795767A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103371216A (en) * | 2012-04-24 | 2013-10-30 | 西姆莱斯有限公司 | Metal-complexing aroma compounds for use in aroma stabilization |
| EP2657326A1 (en) * | 2012-04-24 | 2013-10-30 | Symrise AG | Use of metal ion complexing compounds for aroma stabilisation |
| EP3290498A1 (en) | 2012-04-24 | 2018-03-07 | Symrise AG | Use of metal ion complexing compounds for aroma stabilisation |
| CN107996662A (en) * | 2012-04-24 | 2018-05-08 | 西姆莱斯有限公司 | Complexing of metal ion compound is used for the purposes of flavor-stable |
| CN103371216B (en) * | 2012-04-24 | 2018-11-09 | 西姆莱斯有限公司 | Complexing of metal ion compound is used for the purposes of flavor-stable |
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