CN1793909A - Method of detecting pesticide residue using sensitive insect - Google Patents
Method of detecting pesticide residue using sensitive insect Download PDFInfo
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- CN1793909A CN1793909A CN 200510048720 CN200510048720A CN1793909A CN 1793909 A CN1793909 A CN 1793909A CN 200510048720 CN200510048720 CN 200510048720 CN 200510048720 A CN200510048720 A CN 200510048720A CN 1793909 A CN1793909 A CN 1793909A
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- pesticide residue
- insect
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Abstract
A method of using sensitive insect to detect pesticide residue includes preparing solution of cabbage leaf to be tested, filling prepared solution into atomizing device, placing 30 - 50 sensitive test insects in transparent cup and placing food for test insect, atomizing towards cup with atomizing density of 4.40e - 5ml / mm2 -4.45e-5ml / mm2 , recording dead number of insect after six hours and confirming that pesticide residue in tested sample is toxicity if death rate is not less than 30% and confirming that tested sample is safe if death rate in not greater that 30%.
Description
Technical field:
The present invention relates to a kind of vegetable pesticide residue detection method, belong to the plant protection field.
Background technology:
At present, the Detecting Pesticide technology mainly contains instrumental method (gas chromatography, liquid phase look general, gas chromatography mass spectrometry etc.), enzyme suppresses method (fast detecting case), biology sensor method, immunoassay, live body detection method etc.It is with chromatogram (comprising gas chromatography and liquid chromatography) that the pesticide residue analysis of the overwhelming majority detects, and chromatography can be to the qualitative, quantitative accurately of the various residues of pesticides in the vegetable sample, science, provides check data reliably, equitably.But, running program relative complex with high content of technology because of it, and need specific experiment condition and personnel to operate with certain professional technique, generally be unsuitable for on-the-spot the detection; Speed measuring tank, quick measuring card, fast measuring instrument etc. belong to enzyme and suppress method, because the enzyme instability, poor repeatability and can only detect organic phosphates and carbamate chemicals for agriculture is at a complete loss as to what to do to the cinerins agricultural chemicals, and can not be provided qualitative and quantitative result.The biology sensor method has enzyme sensor, full cell sensor and immunosensor etc., and highly sensitive, detection time is short, but detectable pesticide variety is limited, and it is higher to develop more difficult cost.Immunoassay, it is the ultramicro method that the antibody antigen reaction is combined with modern means of testing, have advantages such as simple, quick, highly sensitive, high specificity, testing cost be low, only need seldom instrument and equipment and professional training, but its performance history need drop into more fund and long period, and generally only is suitable for analyzing an a kind of agricultural chemicals or a class agricultural chemicals.
Summary of the invention
The present invention has overcome the deficiency of existing Detecting Pesticide method, provides a kind of simple to operate, the method for the comprehensive virulence of one or more residues of pesticides of detection that can extensively popularize with low cost.
The technical solution adopted for the present invention to solve the technical problems is:
1. sample thief dish leaf 5～10 grams grind and place triangular flask;
2. add buffer solution 8～12ml, 30～50min is extracted in vibration;
3. the extract of getting in the step 2 places spraying plant;
4. get responsive 30～50 of worms of examination and put into transparent cup, and add examination worm food, with the spraying plant spraying, its spray flux density is 4.40e-5ml/mm
2～4.45e-5ml/mm
2
5.6h write down dead borer population;
6. residues of pesticides have certain toxicity in mortality ratio＞30% interpret sample, mortality ratio≤30% interpret sample safety.
The invention has the beneficial effects as follows: adopt responsive relatively examination worm to detect residues of pesticides, mainly many according to residues of pesticides in the sample, then residual just many in the extract, responsive examination worm contact back mortality ratio is then high.Toxicity according to residues of pesticides in the mortality ratio judgement sample.Because responsive examination worm is easily raised, the checkout equipment less investment, it is simple to detect step, the layman through a few minutes training promptly can, thereby with low cost, operate understandablely, be convenient to spread in each big vegetables production base and the supermarket and use.
Embodiment
Embodiment one
Get and spray decis agricultural chemicals Chinese leaf 5 grams after 2 days, grind and place triangular flask, add phosphate buffer solution (pH value=6.5) 8ml, 30min is extracted in vibration, extract is placed spraying plant, and worm---30 of houseflies are put into spraying plant to get responsive examination, and add food, with the spraying plant spraying, its spray flux density is 4.40e-5ml/mm
2, the dead borer population of record behind the 6h.Mortality ratio=92.4%＞＞30%, residues of pesticides have stronger toxicity in the interpret sample, and are dangerous.
Embodiment two
Get and spray DDVP agricultural chemicals cabbage leaves 10 grams after 30 days, grind and place triangular flask, add phosphate buffer solution (pH value=6.8) 12ml, 50min is extracted in vibration, extract is placed spraying plant, and worm---30 of houseflies are put into spraying plant to get responsive examination, and add food, with the spraying plant spraying, its spray flux density is 4.45e-5ml/mm
2, the dead borer population of record behind the 6h.Mortality ratio=3.5%＜30% is safe in the interpret sample.
Embodiment three
Get and spray Belmark agricultural chemicals Chinese cabbage leaf 8 grams after 4 days, grind and place triangular flask, add phosphate buffer solution (pH value=6.8) 10ml, 40min is extracted in vibration, extract is placed spraying plant, and worm---30 of houseflies are put into spraying plant to get responsive examination, and add food, with the spraying plant spraying, its spray flux density is 4.42e-5ml/mm
2, the dead borer population of record behind the 6h.Mortality ratio=45.5%＞30% has certain residues of pesticides in the interpret sample.
Claims (2)
1. the method for a detecting pesticide residue using sensitive insect may further comprise the steps:
(1) sample thief dish leaf 5～10 grams grind and place triangular flask;
(2) add buffer solution 8～12ml, 30～50min is extracted in vibration;
(3) extract of getting in the step (2) places spraying plant;
(4) get responsive 30～50 of worms of examination and put into transparent cup, and add examination worm food, with the spraying plant spraying, spray flux density is 4.40e-5ml/mm
2～4.45e-5ml/mm
2
(5) 6h writes down dead borer population;
(6) residues of pesticides have certain toxicity in mortality ratio＞30% interpret sample, mortality ratio≤30% interpret sample safety.
2. the method for detecting pesticide residue using sensitive insect according to claim 1, it is characterized in that: damping fluid is made up of the phosphate buffer solution of pH value=6.5～6.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510048720 CN1793909A (en) | 2005-12-22 | 2005-12-22 | Method of detecting pesticide residue using sensitive insect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510048720 CN1793909A (en) | 2005-12-22 | 2005-12-22 | Method of detecting pesticide residue using sensitive insect |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1793909A true CN1793909A (en) | 2006-06-28 |
Family
ID=36805476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510048720 Pending CN1793909A (en) | 2005-12-22 | 2005-12-22 | Method of detecting pesticide residue using sensitive insect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1793909A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101451983B (en) * | 2008-12-25 | 2012-07-04 | 同济大学 | Method for detecting generation toxicity of medicament and personal cure by Caenorhabditis elegans |
| CN104198678A (en) * | 2014-09-17 | 2014-12-10 | 山东农业大学 | Set of methods suitable for evaluating toxicity changes of pesticide residues in soil |
| CN107560916A (en) * | 2017-10-31 | 2018-01-09 | 黄家寅 | The automatic pretreating device of residues of pesticides |
| CN108398524A (en) * | 2018-01-15 | 2018-08-14 | 宁波高新区鹏博科技有限公司 | A kind of detection device of agricultural insecticide effect |
-
2005
- 2005-12-22 CN CN 200510048720 patent/CN1793909A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101451983B (en) * | 2008-12-25 | 2012-07-04 | 同济大学 | Method for detecting generation toxicity of medicament and personal cure by Caenorhabditis elegans |
| CN104198678A (en) * | 2014-09-17 | 2014-12-10 | 山东农业大学 | Set of methods suitable for evaluating toxicity changes of pesticide residues in soil |
| CN107560916A (en) * | 2017-10-31 | 2018-01-09 | 黄家寅 | The automatic pretreating device of residues of pesticides |
| CN108398524A (en) * | 2018-01-15 | 2018-08-14 | 宁波高新区鹏博科技有限公司 | A kind of detection device of agricultural insecticide effect |
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