CN1793865A

CN1793865A - Combined detection reagent for leukorrhea-pH, amine test

Info

Publication number: CN1793865A
Application number: CN 200510022508
Authority: CN
Inventors: 许国荣; 史美宣
Original assignee: Individual
Current assignee: Individual
Priority date: 2005-12-22
Filing date: 2005-12-22
Publication date: 2006-06-28

Abstract

An integrated detection reagent of leukorrhea PH and amine test consists of PH reagent prepared by methyl red and amine test reagent prepared by potassium iodode, iodine and mercury. The advantages are easy operation, without special equipment shot time for testing and low cost.

Description

Leukorrhea-pH, amine test combined detection reagent

Technical field

The present invention relates to a kind of leukorrhea-pH, amine test combined detection reagent, be used for the mensuration of women's bacterial vaginosis BV, belong to the medical reagent technical field.

Background technology

Bacterial vaginosis BV is called for short BV, is in the woman vagina due to the flora imbalance, is one of gynemetrics's common disease, and its assay method has proline aminopeptidase method, sialic acid enzyme process and AmselShi method.Wherein the Amsel method is the goldstandard of generally acknowledging in the world.This method seems simply, but implements not a duck soup, and therefore, domestic most hospitals do not carry out as yet.

The women who suffers from BV, leucorrhea increasing is often with foreign odor, and metritis, adnexitis, box chamber inflammation, ectopic pregnancy, disease such as infertile take place in BV patient easily; The pregnant woman who suffers from BV is easy to generate sense of organization chorion inflammation, and amniotic fluid infects and multiple pregnancy badness disease; In the stream of people or broken palace product, suffer from the women of BV, be easy to generate postoperative infection; Through investigation, BV and gynecological tumor have certain relation.Therefore prevent and treat BV, healthy very important to what shield women.

For the diagnosis of BV, clinically at present Amsel diagnosises that adopt domesticly often are called the goldstandard that " four of leukorrhea " is the diagnosis BV that generally acknowledges in the world more, have in following four promptly that three positives are diagnosable to be BV:(1) vagina PH＞4.5.(2) the amine test positive promptly adds 10% potassium hydroxide and produces stench flavor on vaginal fluid.(3) cell that picks up scent in the smear.(4) vaginal fluid is thinning a foreign odor.PH measures and generally uses test paper method, but the color between scraps of paper PH4 and the PH5 is very approaching, has any problem in correct judgement; Discriminating for amine test varies with each individual, and along with the raising of bio-safety requirement and the reinforcement of sense of self-protection, the reviewer is unwilling to hear this stink.Although recommended the Amsel diagnostic method on gynemetrics's textbook, the hospital that implements is few.

Summary of the invention

Purpose of the present invention is at having any problem in the judgement of the existing Amsel diagnosis that adopts clinically with the existence of test paper mensuration pH value, amine test needs the reviewer to hear, has the deficiency that varies with each individual, a kind of leukorrhea-pH, the amine test combined detection reagent that provide pH value and amine test to judge easily.

The objective of the invention is to be achieved through the following technical solutions, a kind of leukorrhea-pH, amine test combined detection reagent is characterized in that described combined detection reagent comprises with the methyl red and are the PH reagent of major ingredient preparation and are the amine test reagent of major ingredient preparation with potassium iodide, iodine, mercury;

PH reagent is dissolved in the ethanolic solution of 300ml unit quantity with the methyl red of 0.09-0.11g unit quantity, and the physiological saline that adds again behind the distilled water of 200ml unit quantity with equivalent is mixed into;

Amine test reagent with potassium iodide, iodine, mercury by 1: 0.7-0.75: 1 weight proportion adds the dissolving of an amount of distilled water; The pistac liquid that forms after the dissolving is added distilled water become stoste; With stoste, 10% sodium hydroxide solution and distilled water by 1: 4.5-4.75: 1 capacity is mixed into application liquid; Get equivalent application liquid at last and mix formation with 20% potassium hydroxide solution.

The difficult vaginal fluid PH that grasps of existing gynaecology is measured in the present invention and amine test improves, smell with one's nose by test paper method with by the reviewer, the reagent that changes into two kinds of preparations detects, it is the boundary line that PH reagent is measured with PH4.5, PH4.5 is following to be red, and PH4.5 is above to be salmon pink or orange colour even yellow; Amine test reagent is measured, and observes under available light, and colourless or little yellow is negative, and yellow positive, buff is a strong positive.Detecting operation is easy, need not specific installation, and the result is very easy to judge that maximum two minutes of detection time, this reagent cost is lower, is about the 20-30% of above-mentioned other inspections.Be more suitable for generally investigating in large tracts of land, as substituting other inspections, can for the country and the patient save enormous wealth, however, in case apply, year consumption also can reach more than one hundred million units, as in conjunction with plate coating checking, can also diagnose the BV transition state, this helps the prevention of BV, diagnosis early and treatment BV healthyly have a crucial social effect to what ensure numerous women.

Embodiment

Further specify the present invention in conjunction with the embodiments, the present invention includes with the methyl red and be the PH reagent of major ingredient preparation and be the amine test reagent of major ingredient preparation with potassium iodide, iodine, mercury; The preparation of PH reagent, the physiological saline that the ethanolic solution that is dissolved in the 300ml unit quantity with the methyl red of 0.09-0.11g unit quantity adds behind the distilled water of 200ml unit quantity with equivalent again is mixed into, during use, get 2 of PH reagent, about 0.1ml in test tube, the PH developmental tube; The preparation of amine test reagent, use potassium iodide, iodine, mercury is by 1: 0.7-0.75: 1 weight proportion, present embodiment potassium iodide 150g, iodine 110g, mercury 150g, during preparation, elder generation is with the potassium iodide 150g of said ratio, iodine 110g and 100mL distilled water are placed on to be waited in the container to dissolve, add mercury 150g jolting, liquid is become faint yellow by brownish red, become pistac at last, can be dipped in the cold water during Fa Gaore and shake, the pistac liquid that the dissolving back is formed adds distilled water to 2000ml, get above-mentioned stoste 750ml, 10% sodium hydroxide solution 3500ml and distilled water 750ml are mixed into application liquid, get equivalent application liquid and 20% potassium hydroxide solution at last and are mixed into, during use, get 2 of amine test reagent, about 0.1ml in test tube, the amine test pipe.

Vaginal fluid PH measures, and puts into the physiological saline about 2ml in small test tube.The cotton swab that picks leukorrhea is placed in the salt solution fully stirs evenly, sucking-off 0.5ml suspension shakes up in the PH small test tube.Reagent develops the color immediately, shows red expression PH less than 4.5, shows orange colour or yellow expression PH greater than 4.5.

The vaginal fluid amine test, sucking-off 0.5ml suspension adds in the amine test pipe from the leukorrhea suspension, shakes up, and observes (without daylight lamp) under available light, and colourless or little yellow is negative, and yellow positive, buff is a strong positive.

The comparison of two kinds of assay methods

The 486 routine outpatients of gynemetrics use traditional AmselShi method and improved AmseiShi method (reagent method) to check out BV134 example (classic method 84 examples respectively, reagent method 130 examples), in positive 84 examples of the BV that detects with classic method, it is the BV feminine gender with the inspection of reagent method that 4 examples are arranged, and among the BV patient who checks out with the reagent method, with the classic method inspection 50 examples being arranged is the BV feminine gender, through X ²Statistics P＜0.005 shows the positive findings difference of two kinds of methods, shows that the BV positive that the reagent method measures is higher, and the coincidence rate of two kinds of methods is 88.89% by statistics, is fit to clinical practice.

Claims

1, a kind of leukorrhea-pH, amine test combined detection reagent is characterized in that described detectable comprises with the methyl red and are the PH reagent of major ingredient preparation and are the amine test reagent of major ingredient preparation with potassium iodide, iodine, mercury.

2, leukorrhea-pH according to claim 1, amine test combined detection reagent, it is characterized in that PH reagent is dissolved in the ethanolic solution of 300ml unit quantity with the methyl red of 0.09-0.11g unit quantity, the physiological saline that adds again behind the distilled water of 200ml unit quantity with equivalent is mixed into.

3, leukorrhea-pH according to claim 1, amine test combined detection reagent, it is characterized in that amine test reagent with potassium iodide, iodine, mercury by 1: 0.7-0.75: 14 weight proportions add the dissolving of an amount of distilled water; The pistac liquid that forms after the dissolving is added distilled water become stoste; With stoste, 10% sodium hydroxide solution and distilled water by 1: 4.5-4.75: 1 capacity is mixed into application liquid; The application liquid of getting equivalent at last mixes formation with 20% potassium hydroxide solution.

4, according to claim 1 or 3 described leukorrhea-pHs, amine test combined detection reagent, it is characterized in that amine test reagent is when preparation, first potassium iodide with proportioning, iodine and an amount of distilled water are placed on to be waited in the container to dissolve, add the mercury jolting of proportioning again, be pistac liquid at last, can be dipped in the cold water during Fa Gaore in the jolting process and shake.