CN1784977A - Insect rhabdovirus insecticide and its prepn. method - Google Patents
Insect rhabdovirus insecticide and its prepn. method Download PDFInfo
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Abstract
An insecticide is proportionally prepared from the baculovirus of insect and surfactant through separating baculovirus from insect, purifying, mixing it with surfactant, freeze drying and pulverizing.
Description
Technical field
The present invention relates to a kind of insect baculovirus pesticides, relate in particular to the insect baculovirus pesticides preparation and the method for making thereof of a kind of ultralow consumption, high-load.
Background technology
Because agriculture and forestry injurious insect generally produces resistance to chemical insecticide at present, the unit are consumption of chemical insecticide is more and more higher, to environment, non-target animal, bio-diversity and human own, the harm that causes is also more and more serious, a large amount of severe toxicity, high residue insecticide by Chinese government's limited production, cut-off, limit the use of and forbid.Under this background, insect virus pesticide as exploitation is early used wider biopesticide, the situation that it is produced, development and application is fast development, and market demand rises year by year.
Utilizing insect viruses is effective means environmentally safe, that the sustainable Control insect is caused harm as biological insecticides control agriculture and forestry injurious insect.Since the U.S. first insect viruses product E lcar registration in 1975, existing in the world country's 30 various insects viral insecticide products registration above 20.
Because insect viruses are biological products that a class has life, in that former medicine is processed into the process of preparation from virus, protect its insecticidal activity to be without prejudice and are even more important.For example in alkaline environment, the polyhedrin of virus can very fast dissolving, and the activity of virus is destroyed; In the medium-term and long-term preservation of the aqueous solution, very easily produce assorted bacterium in the product, have influence on the quality and the use of product.Thereby develop be suitable for storing, transportation, and the viral insecticide formulation product of effect stability, most important to the large-scale promotion application of this class biopesticide.
Compare with chemical insecticide, the kind of insect baculovirus pesticides exploitation is few, and in evening time that research and development are carried out, research basis and level are relatively low.The research and development of PI toxin preparation all are that technology and the method for using for reference chemical insecticide are carried out, yet the physicochemical property of insect baculovirus and insecticidal mechanism have distinct characteristics, some technology of imitation chemical insecticide processing, for the performance of insect viruses insecticidal action, be unnecessary or even harmful.
The formulation of having registered at present the insect virus pesticide product mainly contains two kinds of wetting powder and suspending agents, virus polyhedron content is generally at 5-20 hundred million PIB/mL (gram), viral level is low in the product, the inserts too high levels, field dosage big (general field mu consumption 40-100mL (gram) or more than), make troubles in packing, store, transportation, sell, and product a series of problems such as denaturation of virus inactivation in the producing and selling process.The for example processing of wetting powder in the viral insecticide, with reference to the technology of conventional chemical insecticide wetting powder, add the inertia filler of a large amount of (60%-90%), as diatomite, kaolin etc., caused the process dust pollution like this, viral level reduces in the preparation.With the heliothis armigera nuclear polyhedrosis virus is example, the content of former medicine is at 100-200 hundred million PIB/mL (gram), when process preparation, a large amount of fillers and a certain amount of auxiliary agent have been added, thereby the polyhedron content that makes the heliothis armigera nuclear polyhedrosis virus preparation is between 10-20 hundred million PIB/mL (gram), to being diluted in about 10 times of former concentration.For example existing wetting powder product has, the Henan Province rich biochemical pesticide of universal love favour Co., Ltd registration product: 1,000,000,000 PIB/ gram heliothis armigera nuclear polyhedrosis virus wetting powder, registration card number: LS94579; Chibi, the Hubei Province sincere biotechnology of will Co., Ltd registration product: 1,000,000,000 PIB/ gram heliothis armigera nuclear polyhedrosis virus wetting powder, registration card number: LS20011685 etc.The suspending agent product has, Shijiazhuang City, Hebei Province city ring industry biopesticide factory registration product: 2,000,000,000 PIB/ milliliter heliothis armigera nuclear polyhedrosis virus suspending agents, registration card number: LS96795; The registration product of Zhuhai City, Guangdong Province China biologic product Co., Ltd: 2,000,000,000 PIB/ milliliter heliothis armigera nuclear polyhedrosis virus suspending agents, registration card number: LS20031797 etc.
Wetting powder and suspending agent respectively have pluses and minuses in transportation and application: be not easy to the preservation transportation as suspending agent, though and wetting powder is better than suspending agent aspect active keeping viral organism, but because under the situation of preparation thin up when using in the field, the technical merit that is added man-hour limits, the polyhedrosis dispersiveness of virus is good not as suspending agent, thereby causes the field effect to be lower than suspending agent.The polyhedron of nuclear polyhedrosis virus is the solid granule of particle diameter between 0.5-10 μ m, is different from the former medicine of chemical pesticide, and the performance of its insecticidal activity is decided by the degree of scatter of viral polyhedron particle in water to a great extent.After scattered preparation dilutes according to a certain percentage, can reach the concentration requirement of regulation, when spraying in the field, with the polyhedron uniform particles be distributed on the crop, increased the chance of insect contact virus and got the quantity of food virus; On the contrary, disperse bad preparation can not reach above-mentioned effect, thereby greatly reduce the effect of field control.
Summary of the invention
The objective of the invention is to overcome existing wetting powder dispersed bad in water, and suspending agent is not easy to the defective of preservation transportation, thereby a kind of good dispersion, easily preservation, convenient transportation are provided, and the few insect baculovirus pesticides of field consumption, and preparation method thereof.
Insect baculovirus pesticides provided by the invention is a solid dosage forms, comprises viral freeze-dried powder and surfactant, and the amount of surfactant is not less than 1/10th of viral freeze-dried powder weight.
Wherein baculoviral comprises nuclear polyhedrosis virus and PuGV.In the insect virus pesticide of the present invention, viral level is preferably 100-5000 hundred million PIB/ gram.
Described surfactant can be an anion surfactant as auxiliary agent, cationic surfactant, amphoteric surfactant and non-ionic surface active agent; Anion surfactants such as dodecyl sodium laurate, neopelex, lauryl sodium sulfate, lignin sodium, lignin calcium more preferably.
The invention provides a kind of preparation method of above-mentioned insect baculovirus pesticides, comprise the steps:
(1) will separate the insect viruses stoste obtain of purifying with the abundant mixing of certain amount of surfactant, surfactant is dissolved in the moisture around the virus.During practical operation,, determine to need to add the ratio of surfactant according to the content of viral former medicine after requirement that designs production formulation content and the freeze drying; Content such as virus after the freeze drying is 5,000 hundred million PIB/ grams, need to produce the preparation of 1,000 hundred million PIB/ gram, so viral former medicine is 1: 4 with the weight ratio that surfactant adds, the viral former medicine that promptly is equivalent to after 1 part of freeze-drying adds 4 parts of surfactants, and wherein the addition of surfactant is not less than 1/10th of viral former medicine weight after the freeze-drying.
(2) with virus and surfactant mixtures freeze drying; Can obtain insect baculovirus pesticides of the present invention.
In order further to improve the dispersive property of preparation, this preparation method can comprise the steps further that also (3) are crushed to the 325-1000 order with cracker with the cryodesiccated mixture that step (2) obtains, and obtain designing the viral insecticide of content.
Insect baculovirus pesticides provided by the invention can use separately, also can unite use with chemical insecticide, is applied to prevent and treat agriculture and forestry injurious insect.For example, can be with chemical insecticide---fluorine bell urea, fluorine pyridine urea or Affirm (Merck Co.), and insect baculovirus pesticides---laphygma exigua nuclear polyhedrosis virus insecticide, prodenia litura nuclear polyhedrosis virus insecticide, heliothis armigera nuclear polyhedrosis virus insecticide or plutella xylostella granulosis virus insecticide are united use.
Because the character of insect baculovirus polyhedron surface protein, if the polyhedron gathering of seriously luming when convection drying can cause in water, and the polyhedron surface is not easy by water infiltration, badly influences the dispersiveness in water.And method provided by the invention is promptly to add surfactant before viral drying, make it to be dissolved in the viral polyhedron water on every side, around polyhedron, form the hydrophilic layer of forming by surfactant, after the freeze drying, surfactant is wrapping up polyhedrosis each particle, in case contact wetting once more, the dissolving of polyhedron circumferential surface activating agent has reduced the surface tension of polyhedron ambient water molecule, force again simultaneously between the polyhedron mutually away from dispersion, reach the dispersion effect of extraordinary single virus in water.When using,, determine the extension rate of preparation according to the usage amount in different virus field, the content and the field duty of water of preparation virus.Thereby insect baculovirus pesticides provided by the invention, compare with existing wetting powder, its degree of scatter in water is very high, even cover crop surface when viral polyhedron particle is used in the field, give full play to the insecticidal effect of virus, so when using in the field, consumption significantly reduces, every mu of preparation consumption gets final product between the 0.2-10 gram.Up to now also do not see the virus formulation and the correlative study of the high-load of announcing as the present invention like this.In addition, insect baculovirus pesticides provided by the invention is a kind of solid pharmaceutical preparation, is convenient to the preservation transportation than suspending agent.
The advantage of insect baculovirus pesticides provided by the invention:
1. owing to the viral level height, when therefore using this insecticide, the field consumption descends greatly, and every mu of preparation consumption is between the 0.2-10 gram.For example be the virus of 1,000 hundred million PIB for the mu consumption, when the preparation viral level restrained at 10,000,000,000 PIB/, every mu of consumption was 10 grams; When the preparation viral level restrained at 5,000 hundred million PIB/, every mu of consumption was 0.2 gram.
2. examine under a microscope, the preparation of 40,000,000,000 PIB/ gram, when diluting 40000 times, the polyhedron that is the simple grain dispersion accounts for more than 95%, and the polyhedron of assembling caking is less than 5%.
3. but insect baculovirus pesticides of the present invention is long preservation at normal temperatures, and virus can the sex change inactivation, and convenient transportation.
Embodiment
Embodiment 1
Determine virus stock solution used freeze-drying multiple: from content is the prodenia litura nuclear polyhedrosis virus stoste of 20,000,000,000 PIB/ gram (production of Jiyuan, Henan Province white clouds Industrial Co., Ltd.), extract 100 gram sample freeze dryings, getting 30 gram content is the virus of 66,700,000,000 PIB/ gram, and the multiple of then viral former medicine freeze-drying is 3.3 times.
Preparation insect baculovirus pesticides: in the virus stock solution used of the above-mentioned 20,000,000,000 PIB/ gram of 1000 grams, add 33 gram neopelexes, fully stir, neopelex is dissolved in the moisture between the virus, freeze drying obtains the prodenia litura nuclear polyhedrosis virus insecticide that 333 grams, 60,000,000,000 PIB/ restrain.
From above-mentioned viral insecticide, extract 0.1 gram sample, add water, stir evenly to 1000mL (diluting 10000 times), the microscopically counting, in per 200 viral polyhedroies, it is that simple grain disperses that 198 polyhedroies are arranged, dispersion rate is 99.0%.
If using the prodenia litura nuclear polyhedrosis virus amount by cabbage field control prodenia litura is that 1,000 hundred million PIB add 50 kilograms of/mu calculating of water, so every mu only needs this insect baculovirus pesticides 1.3 grams, adds 50 kilograms in water with 40,000 times of sprayings of it dilution.
Embodiment 2
Determine virus stock solution used freeze-drying multiple: the content that obtains from separation and purification is (production of Jiyuan, Henan Province white clouds Industrial Co., Ltd.) the heliothis armigera nuclear polyhedrosis virus stoste of 1,000 hundred million PIB/ gram, extract 100 gram sample freeze dryings, getting 20 gram content is the virus of 5,000 hundred million PIB/ gram, and the multiple of then viral former medicine freeze-drying is 5 times.
Preparation insect baculovirus pesticides: in the virus stock solution used of the above-mentioned 1,000 hundred million PIB/ gram of 3000 grams, add 2400 gram neopelexes, fully stir, neopelex is dissolved in the moisture between the virus, freeze drying obtains the heliothis armigera nuclear polyhedrosis virus insecticide that 3000 grams, 1,000 hundred million PIB/ restrain.
From above-mentioned insect baculovirus pesticides, extract 0.1 gram sample, add water, stir evenly to 1000mL (diluting 10000 times), the microscopically counting, in per 200 viral polyhedroies, it is that simple grain disperses that 195 polyhedroies are arranged, dispersion rate is 97.5%.
If using the heliothis armigera nuclear polyhedrosis virus amount by cotton field control cotton bollworm is that 1,000 hundred million PIB add 50 kilograms of/mu calculating of water, so every mu only needs this insect baculovirus pesticides 1 gram, adds 50 kilograms in water with 50,000 times of sprayings of it dilution.
Embodiment 3
Determine virus stock solution used freeze-drying multiple: from separation and purification content is the laphygma exigua nuclear polyhedrosis virus stoste of 14,000,000,000 PIB/ gram (production of Jiyuan, Henan Province white clouds Industrial Co., Ltd.), extract 100 gram sample freeze dryings, getting 15.6 gram content is the virus of 90,000,000,000 PIB/ gram, and the multiple of then viral former medicine freeze-drying is 6.43 times.
Preparation insect baculovirus pesticides: in the virus of the above-mentioned 14,000,000,000 PIB/ gram of 10000 grams, add 440 gram lignin sodium, fully stir, lignin sodium is dissolved in the moisture between the virus, freeze drying, the freeze-dried powder that obtains is crushed to 1000 orders with airslide disintegrating mill, makes the laphygma exigua nuclear polyhedrosis virus insecticide of 2000 grams, 70,000,000,000 PIB/ gram.
From above-mentioned viral insecticide, extract 0.1 gram sample, add water, stir evenly to 500mL (diluting 5000 times), the microscopically counting, in per 140 viral polyhedroies, it is that simple grain disperses that 136 polyhedroies are arranged, dispersion rate is 97.1%.
If using the laphygma exigua nuclear polyhedrosis virus amount by vegetables field control beet armyworm is that 60,000,000,000 PIB add 50 kilograms of/mu calculating of water, so every mu only needs this insect baculovirus pesticides 0.86 gram, adds 50 kilograms in water with 50,000 8 thousand times of sprayings of it dilution.
Embodiment 4
Determine virus stock solution used freeze-drying multiple: the former liquid hold-up of the plutella xylostella granulosis virus of separation and purification is 6,000 hundred million PIB/ grams (production of Jiyuan, Henan Province white clouds Industrial Co., Ltd.), from wherein extracting 100 gram freeze dryings, getting 34 gram content is the viral former powder of 24,776 hundred million PIB/ gram, and then the freeze-drying multiple of viral former powder is 2.9 times.
The preparation insect baculovirus pesticides: adding 860 gram lauryl sodium sulfate in the plutella xylostella granulosis virus of the above-mentioned 6,000 hundred million PIB/ gram of 1000 grams, fully stir, lauryl sodium sulfate is dissolved in the virus water on every side.Obtain the plutella xylostella granulosis virus insecticide of 1200 grams, 5,000 hundred million PIB/ gram after the freeze drying.
Microscopic examination, 10,000 times of water-reducible insect baculovirus pesticides, the dispersion rate of its PuGV reaches 95.2%.
Embodiment 5
Determine virus stock solution used freeze-drying multiple: from separation and purification content is the laphygma exigua nuclear polyhedrosis virus stoste of 14,000,000,000 PIB/ gram, extract 100 gram sample freeze dryings, getting 15.6 gram content is the virus of 90,000,000,000 PIB/ gram, and the multiple of then viral former medicine freeze-drying is 6.43 times.
In the virus of the above-mentioned 14,000,000,000 PIB/ gram of 10000 grams, add 440 gram lignin sodium, fully stir, lignin sodium is dissolved in the moisture between the virus, freeze drying, the freeze-dried powder that obtains is crushed to 325 orders with airslide disintegrating mill, makes the laphygma exigua nuclear polyhedrosis virus insecticide of 2000 grams, 70,000,000,000 PIB/ gram.
The laphygma exigua nuclear polyhedrosis virus insecticide of above-mentioned 70,000,000,000 PIB/ gram is carried out biologicall test indoor.Concrete assay method and step are described below:
Test insect: the beet exigua larvae in 2 latter stages in age;
The preparation of test medicament: with running water the laphygma exigua nuclear polyhedrosis virus insecticide that 70,000,000,000 PIB/ restrain is carried out gradient dilution, extension rate is respectively: 7,000,14,000,70,000,140,000,700,000,7,000,000 times, obtain content and be respectively 1 * 10
7, 5 * 10
6, 1 * 10
6, 5 * 10
5, 1 * 10
5, 1 * 10
4The concentration gradient of the series of PIB/mL is made blank with running water;
Assay method: (the artificial feed prescription is seen: Li Guanghong to pour the beet armyworm artificial feed in foster worm ware, Chen Qijin, Pang Yi, 2000, the insect journal, 43:356-363), after feed solidifies, pour the viral suspension of above-mentioned medicament respectively into, make it submergence feed surface, left standstill 1 minute, and poured out whole viral suspensions, the air-dry feeds surface.Access beet exigua larvae in 2 latter stage in age in foster worm ware, every ware inserts 30, adds to support worm ware lid on the lid layer paper handkerchief bonnet, and at 26 ± 0.5 ℃, L: D=16: 8 (L: D is the ratio of periodicity of illumination, and L represents lighting delay number, number when D represents dark) are raising down.After 24 hours, will try worm and divide in the 24 hole insect box, 1 artificial feed (about 2 grams) and 1 beet exigua larvae are put in every hole, and similarity condition is raised down, the death condition of 72 hours " Invest, Then Investigate " examination worms.Each insect box is total to triplicate, i.e. 72 larvas of each concentration determination of every kind of medicament as a repetition;
Measurement result: the 4th day LC behind the medicine
50=66900PIB/mL, correlation coefficient r=0.964, virulence equation y=-2.95+1.65x.
Correspondingly, with the former medicine of laphygma exigua nuclear polyhedrosis virus of 1,200 hundred million PIB/ gram in the indoor biologicall test that the beet exigua larvae in 2 latter stages in age is carried out (assay method and step are the same).The result is the 4th day LC behind the medicine
50=208000PIB/mL, correlation coefficient r=0.919, virulence equation y=-0.97+1.12x.
From above-mentioned data as can be seen, insect baculovirus pesticides causes insecticidal effect to be better than the effect of former medicine, with LC owing to have preferably dispersedly
50Calculate, effect has improved 3.1 times.
Embodiment 6
Determine virus stock solution used freeze-drying multiple: the content that obtains from separation and purification is the prodenia litura nuclear polyhedrosis virus stoste of 20,000,000,000 PIB/ gram, extract 100 gram sample freeze dryings, getting 30 gram content is the virus of 66,700,000,000 PIB/ gram, and the multiple of then viral former medicine freeze-drying is 3.3 times.
Preparation insect baculovirus pesticides: in the virus stock solution used of the above-mentioned 20,000,000,000 PIB/ gram of 1000 grams, add 33 gram neopelexes, fully stir, neopelex is dissolved in the moisture between the virus, freeze drying obtains the prodenia litura nuclear polyhedrosis virus insecticide (hereinafter referred to as viral single agent) that 333 grams, 60,000,000,000 PIB/ restrain.
Insecticidal spectrum, raising desinsection speed, quickening desinsection time in order to expand viral insecticide of the present invention, can also be hybridly prepared into the viral insecticide that contains chemical insecticide with chemical insecticide.Present embodiment selects for use chemical insecticide fluorine bell urea to be mixed.Fluorine bell urea is a kind of insect growth regulator, IGR insecticides of high-efficiency low-toxicity, and lepidoptera pest is had the better prevention effect, adds in viral insecticide, can reach the double purpose of controlling, control soon, remedy the viral insecticide shortcoming.
Preparation contains the insect baculovirus pesticides of chemical insecticide: prodenia litura nuclear polyhedrosis virus insecticide 200 grams of getting the above-mentioned 60,000,000,000 PIB/ gram that has prepared, former medicine 100 grams of the fluorine bell urea of adding 98.4%, after thick the mixing, pulverize through airslide disintegrating mill again, get 300 gram mixed type insect virus pesticides (mixture), wherein prodenia litura virus polyhedron content is that the weight percentage of 40,000,000,000 PIB/ gram, fluorine bell urea is 32.6%.
Mix the cabbage field that takes place with every mu 3 consumption control prodenia litura and beet armyworm that restrains above-mentioned mixed type insect virus pesticide, respectively with the prodenia litura nuclear polyhedrosis virus insecticide (viral single agent) of every mu 2 60,000,000,000 PIB/ gram that restrains and 5% 5 percent of hexaflumuron emulsifiable concentrate (the chemical insecticide list agent of every mu of 19.56mL, Jiangsu Yangnong Chemical Group Co., Ltd's production) compare medicament, control efficiency such as following table.
Virus mixture and single agent thereof are to the comparison of cabbage field pest controling effect
| The control efficiency of two kinds of insects of different number of days behind the medicine | 1 day | 6 days | ||
| Beet armyworm | Prodenia litura | Beet armyworm | Prodenia litura | |
| Mixture (mu consumption: prodenia litura virus 1,200 hundred million PIB fluoridize bell ureas 0.978 gram) | 43.5％ | 47.2％ | 75.6％ | 92.3％ |
| The single agent of virus (mu consumption: prodenia litura virus 1,200 hundred million PIB) | 1.2％ | 2.5％ | 2.6％ | 80.3％ |
| Chemical insecticide list agent (mu consumption: fluorine bell urea 0.978 gram) | 10.5％ | 12.4％ | 14.6％ | 10.7％ |
| The control efficiency that mixture improves than single agent | 31.8％ | 32.3％ | 58.4％ | 1.3％ |
As can be seen from the table, prodenia litura virus is used with chemical insecticide, not only has good synergistic effect, and has enlarged insecticidal spectrum, and beet armyworm is had the better controlled effect.For chemical insecticide fluorine bell urea, conventional field consumption is every mu of pure product 6-8 gram of use, and in the mixture of this experiment, every mu has only been used pure product 0.984 gram, consumption is significantly less than conventional amount used, but because virus with its synergistic function, has obtained more satisfactory field control effect.Owing to the selectivity of virus host, prodenia litura nuclear polyhedrosis virus is only effective to prodenia litura, and is invalid to beet armyworm, behind the fluorine bell urea that has been mixed, obtained the effect of better prevention beet armyworm simultaneously.Thereby insect virus pesticide of the present invention with some low toxicity efficiently chemical pesticide be mixed and use with, help to improve insecticide efficiency, enlarge the desinsection scope, accelerate the desinsection time.
Claims (10)
1. an insect baculovirus pesticides comprises viral freeze-dried powder and surfactant, and the amount of surfactant is not less than 1/10th of viral freeze-dried powder weight.
2. insect baculovirus pesticides as claimed in claim 1 is characterized in that, the viral level in the described viral freeze-dried powder ranges up to and reaches 5,000 hundred million PIB/ gram.
3. insect baculovirus pesticides as claimed in claim 1 or 2 is characterized in that, described virus is nuclear polyhedrosis virus or PuGV.
4. insect baculovirus pesticides as claimed in claim 1 is characterized in that, described surfactant is anion surfactant, cationic surfactant, amphoteric surfactant and non-ionic surface active agent.
5. insect baculovirus pesticides as claimed in claim 4 is characterized in that, described anion surfactant is selected from dodecyl sodium laurate, neopelex, lauryl sodium sulfate, lignin sodium and lignin calcium.
6. the preparation method of the described insect baculovirus pesticides of claim 1 comprises the steps:
1) will separate the insect viruses stoste obtain of purifying with the abundant mixing of certain amount of surfactant, surfactant is dissolved in the moisture around the virus; Wherein the addition of surfactant is not less than 1/10th of viral former medicine weight after the freeze-drying;
2) with above-mentioned virus that obtains and surfactant mixtures freeze drying, obtain insect baculovirus pesticides of the present invention.
7. the preparation method of insect baculovirus pesticides as claimed in claim 6 is characterized in that, also comprises with cracker step 2) the cryodesiccated mixture that obtains is crushed to the 325-1000 order.
8. the application of insect baculovirus pesticides as claimed in claim 1 on the control agriculture and forestry injurious insect.
9. insect baculovirus pesticides as claimed in claim 1 and chemical insecticide are united the application of use on the control agriculture and forestry injurious insect.
10. insect baculovirus pesticides as claimed in claim 9 and chemical insecticide are united the application of use on the control agriculture and forestry injurious insect, it is characterized in that described chemical insecticide is fluorine bell urea, fluorine pyridine urea or Affirm (Merck Co.); Described insect baculovirus pesticides is laphygma exigua nuclear polyhedrosis virus insecticide, prodenia litura nuclear polyhedrosis virus insecticide, heliothis armigera nuclear polyhedrosis virus insecticide or plutella xylostella granulosis virus insecticide.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100546483C (en) * | 2007-11-29 | 2009-10-07 | 中国科学院武汉病毒研究所 | A kind of broad spectrum bacilliform virus insecticide of producing with substituted host |
| CN101366394B (en) * | 2008-08-22 | 2011-02-16 | 苏州大学 | Composite biological insecticide |
| CN106857505A (en) * | 2017-03-16 | 2017-06-20 | 河南省济源白云实业有限公司 | Insect nuclear polyhedrosis virus dry suspending agent and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1176213C (en) * | 2002-05-31 | 2004-11-17 | 中国科学院武汉病毒研究所 | Insecticide suspension of beet noctuid polyhedron virus |
-
2004
- 2004-12-10 CN CNB2004100984399A patent/CN1327773C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100546483C (en) * | 2007-11-29 | 2009-10-07 | 中国科学院武汉病毒研究所 | A kind of broad spectrum bacilliform virus insecticide of producing with substituted host |
| CN101366394B (en) * | 2008-08-22 | 2011-02-16 | 苏州大学 | Composite biological insecticide |
| CN106857505A (en) * | 2017-03-16 | 2017-06-20 | 河南省济源白云实业有限公司 | Insect nuclear polyhedrosis virus dry suspending agent and preparation method thereof |
| CN106857505B (en) * | 2017-03-16 | 2020-12-29 | 河南省济源白云实业有限公司 | Insect nuclear polyhedrosis virus dry suspending agent and preparation method thereof |
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| Publication number | Publication date |
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| CN1327773C (en) | 2007-07-25 |
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