CN1773285A - Reagent kit and method for combined testing EPSPS and BT protein - Google Patents
Reagent kit and method for combined testing EPSPS and BT protein Download PDFInfo
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- CN1773285A CN1773285A CN 200510086760 CN200510086760A CN1773285A CN 1773285 A CN1773285 A CN 1773285A CN 200510086760 CN200510086760 CN 200510086760 CN 200510086760 A CN200510086760 A CN 200510086760A CN 1773285 A CN1773285 A CN 1773285A
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Abstract
The present invention relates to a kit for combined detection of EPSPS and BT protein and its method. Said kit is formed from bottom plate and upper cover. In the bottom plate interior two test strips are set, said upper cover has two observation windows and two sample-loading holes. Said kit makes the main body film reaction system of detection system for detecting EPSPS and BT protein be assembled in same kit interior, can be used for simultaneously detecting that the plant seed, leaf and its processed product contain EPSPS or BT crystal processed product.
Description
Technical field:
The present invention relates to the kit of a kind of combined testing EPSPS and BT albumen.The invention still further relates to the method that detects EPSPS and BT albumen with this kit simultaneously.
Background technology:
In the developments of genetically modified plants or transgenic product is being screened or during safety evaluatio, often needing the allogenic gene that changes over to is carried out qualitative or semiquantitative determination.
At present no matter the genetically modified crops that occur are cottons, or corn, soybean, change following two kinds of foreign genes mostly over to:
1. have pesticidal BT crystal protein gene (comprise BT-Cry1Ab or BT-Cry1Ac, abbreviate the BT gene usually as), Bt is Bacillus thuringiensis, i.e. the abbreviation of bacillus thuringiensis; Cry is the abbreviation of crystalline protein (crystalprotein); Cry1Ab and Cry1Ac are two kinds of common insecticidal crystal proteins of bacillus thuringiensis gene code.
2. specificity antiweed EPSPS (5-enol acetone shikimic acid-3-phosphate synthase) gene.
Therefore, in the practice a kind of plant sample is often all needed to carry out the detection of two kinds of genes.
Existing detection technique such as enzyme exempt from, put method such as exempt from, be subjected to the restriction of factors such as instrument (microplate reader, put and exempt from scintiloscope, hydro-extractor etc.), place, and detection time is long, and (the inspection-free survey time of enzyme needs 20hr, put and need to exempt from that 30min～1hr), it is higher to detect cost, is difficult to apply.
Though the method that detects with test strips that occurs at present can partly overcome above deficiency (as U.S. EnviroLogixIncorporated, Strategic Diagnostics, Inc. the test strips of producing with Chongqing Jinbiao Biotech Company Ltd.), but every kind of test strips can only detect foreign gene single in the genetically modified crops, is easy to generate error result in the practical operation.For example: if the tester only has EPSPS genetic test test strips, and do not change the EPSPS gene in the detected object over to, but change the BT gene over to, draw the error result that there is not foreign gene in detected object so possibly, cause unnecessary loss.And when operation, test strips need be immersed in the liquid to be detected, operation is not very convenient.
Therefore, be sought after in the practice a kind ofly can detecting BT crystal protein gene and EPSPS gene in the genetically modified plants simultaneously, and easy and simple to handle, result accurately and reliably, device and detection method fast.
Summary of the invention:
The objective of the invention is to set up a kind of easy and simple to handle, result accurately and reliably, fast, and can detect the method and the detection kit of two kinds of different foreign genes (EPSPS and BT protein gene) simultaneously.
The technical scheme of kit is such: it comprises base plate and loam cake, and base plate cover is formed kit after going into loam cake, it is characterized in that being placed with on the base plate two calibration tapes; On be stamped two observation windows and two wells.
The upper surface of base plate can be provided with two grooves, and every groove is embedded in a calibration tape, and the thickness of base plate is not limit.
Described base plate or loam cake can have draw-in groove, to guarantee base plate and loam cake fastening.
Described observation window is rectangle preferably.
The shape of kit is not limit, as being flat rectangle or square box.
The material of kit can not have the material of influence to sample and calibration tape with plastics or glass etc., and plastics are optimal material, the damage of both can having avoided colliding with, and in light weight, cheap.
Two calibration tapes in the described base plate, one is used to detect EPSPS, and another is used to detect BT albumen.Described calibration tape is a kind of test material with detection system body membrane reaction system function, is made by multilayer materials such as macromolecular fibre film, plastics.The calibration tape of two kinds of albumen all can be divided into Quality Control district and detection zone, and the Quality Control district can wrap by sheep anti-mouse igg or rabbit anti-mouse igg polyclonal antibody; The sample detection district bag of EPSPS genetic test band is by exceptional function EPSPS, and wrap by the monoclonal antibody of BT-Cry 1Ab/Ac or polyclonal antibody in the sample detection district of BT albumen calibration tape.
When foreign gene changes plant over to, and in plant, express, will produce destination protein (EPSPS and BT), therefore determine by whether containing above-mentioned two kinds of albumen in the test sample whether this gene exists.If foreign gene changes plant over to, the producer silence, no destination protein produces, and (desinsection or antiweed) can not accomplish the end in view.
In an example of the present invention, described detection system body membrane reaction system is by forming with the lower part:
M1: reaction film, by the high molecular cellulose film preparation of high protein compatibility, as the carrier and the demonstration reaction result of system response;
M2a, M2b, M3: sample pad, strong water-intake capacity is arranged, can be reactive system suitable reaction conditions is provided, and help to draw sample.
M4: colloid gold label antibody carrier film, as the collaurum pad, absorption colloid gold label antibody and as its solid phase carrier;
M5: adsorptive pads, the sample after the absorption detecting;
M6: back up pad, make by PVC, as the stilt of reaction system;
M7: two-sided and single face adhesive tape, different size can be arranged, be used for fixing each reaction film and absorbent material on M6;
M8: transparency protected adhesive tape, fixing and protection sample pad and collaurum pad;
M9: the color sign adhesive tape, detect the signature identification of strip as this.
Bag is separately fixed in the reagent base plate by good EPSES calibration tape and BT-Cry1Ab/Ac calibration tape, comprises complete film reaction system (M1-M9) in the calibration tape.M1-M9 is that fixedly the collaurum pad adopts the stronger glass fibre of absorption affinity, as the solid phase carrier of golden labelled antibody; Nitrocellulose filter is the high molecular cellulose film (NC film) of high protein compatibility, is prepared as reaction film and is used as the carrier of system response and shows reaction result after pre-service; The absorption of sample pad has (containing reaction auxiliary material pad) glass fibre and the nonwoven fabrics of strong water-intake capacity, after special processing, for reactive system provides suitable reaction conditions, and helps suction; Absorption pad is an adsorptive pads; The PVC base plate is a kind of plastic base, as the stilt of reaction system.
Described high molecular cellulose film M1 can select polymeric membranes such as nitrocellulose filter, cellulose acetate membrane, nylon membrane for use, is prepared as reaction film after pre-service;
Described sample pad M2a, M2b, M3 can select glass fibre or other bibulous stringiness film for use;
Described colloid gold label antibody carrier film M4 can select the stronger glass fibre of absorption affinity or other stringiness film for use, sprays collaurum-antibody complex on carrier film;
Described back up pad M6 is sheet plastic or other hydrophobicity thin plate.
Described colloid gold label antibody is EPSPS or BT-Cry1Ab/Ac monoclonal antibody.
The preparation method of above-mentioned collaurum is: gold chloride (HAuCl
4) under the reductive agent effect, aggregate into the gold grain of a certain size (diameter is at 1-150nm), form electronegative hydrophobic sol solution.Owing to electrostatic interaction becomes stable colloidal state, claim collaurum, belong to heterogeneous heterogeneity system, color is salmon pink to aubergine, for reaction provides naked eyes detectable signal.
Colloid gold label comes down to the big molecule of protein (antibody) and is adsorbed to the bag on colloid gold particle surface by process.Adsorption mechanism may be the colloid gold particle surface negative charge, forms strong bonded with the positive charge group of protein because of Electrostatic Absorption, belongs to physisorption.
Colloid gold label antibody is by the antigen-antibody reaction (double antibody sandwich method: antibody-Ag-Ab), realize the special detection to transgene product of high special.
From the monoclonal antibody of a kind of antigenic determinant or many anti-bags by on reaction film as checking band, from collaurum on the monoclonal antibody mark of another kind of antigenic determinant as developer, with antigen (EPSPS albumen; BT-Cry1Ab1/Ac albumen) be middle couplet, too little naked eyes under the disperse state can't be accumulated on the reaction film by immune response by observed colloid gold particle that making the collaurum color be exaggerated naked eyes can observe.This colour developing mode of collaurum is the physics colour developing, need not substrate and participates in reaction, method simple, intuitive.
The core of detection technique of the present invention is to utilize highly perfect film reaction system, provides to realize the parallel various conditions that move and finish the idiosyncrasy institute palpus of immunoaffinity chromatography of liquid sample, and with EPSPS ﹠amp; The BT-Cry1Ab/1Ac calibration tape is assemblied in the same reagent base plate.EPSPS ﹠amp; BT-Cry1Ab/1Ac joint inspection gold-marking immunity detection kit system uses the macromolecular fibre film as solid phase carrier.This film has very strong adsorption function, EPSPS ﹠amp; After BT-Cry1Ab/1Ac antibody wraps respectively and is handled in different films and drying promptly as solid phase, can with in the liquid phase corresponding antigens-the colloid gold label antibody complex is quick combines.Under moisture state, the antigen in the liquid phase-colloid gold label antibody complex can be done the slewing swimming and combines with specific antibody on the immobilon-p because of " wick drainage phenomenon ", forms the red cement line of special golden labelled antibody-Ag-Ab.
The object of the present invention is achieved like this: after base plate cover is gone into loam cake, each respectively corresponding calibration tape of each observation window and well, can carry out application of sample and observe testing result by well on the lid and observation window, each observation window can be checked a kind of gene respectively.
Each observation window is divided into test section (T) and Quality Control district (C) two parts, can indicate last covering with letter C, T.
During detection, same a kind of extract of inspecting article by ready samples is dripped respectively in two wells of kit, after 3-5 minute, just can observe directly testing result accurately from two observation windows of loam cake by naked eyes.
For each observation window, judge with the following methods testing result (referring to Fig. 4～Fig. 6):
1, negative (-):
Reaction condition: an aubergine band appears in observation window Quality Control district (C).
Testing result: do not contain gene to be checked or its content in the sample to be checked at it below threshold value.
The analysis of causes:
The aubergine band that (C) manifested in the Quality Control district is to judge whether enough plant sample liquid is arranged, and whether normal chromatography process standard simultaneously also as the inner quality standard of reagent.
As not containing specific EPSPS or BT-Cry1Ab/1Ac albumen in the sample, or its concentration is lower than detection sensitivity, colloidal gold antibody in the chromatography process not can be fixed on the film antibody response that detects in the band, thereby (T) an aubergine detection can not occur and be with the test section in.
2, positive (+):
Reaction condition: an aubergine band appears in observation window Quality Control district (C), occurs the aubergine band simultaneously in test section (T).
Testing result: gene content to be checked is more than threshold value.
The analysis of causes:
When if EPSPS or BT-Cry1Ab/1Ac protein concentration are higher than its detection threshold in the plant sample to be measured, antigen immunity gold with detect with on another antibodies, therefore (T) aubergine occurs and detects band in the test section.
3, invalid:
Reaction condition: the aubergine band does not appear in Quality Control district (C),
Testing result: show the rotten damage of incorrect operating process or kit.
The advantage of kit of the present invention and detection method is:
1) high specificity: EPSPS ﹠amp; The detection strip of BT-Cry1Ab/1Ac is all with high specific CP4 EPSPS or BT-Cry1Ab/1Ac Antibody Preparation, and is very low with the cross reaction of other non-destination protein.
2) susceptibility height: EPSPS ﹠amp; The detection strip of BT-Cry1Ab/1Ac is selected EPSPS ﹠amp respectively for use; BT-Cry1Ab/1Ac monoclonal antibody mark collaurum, no covalent bond forms between collaurum and antibody, thereby the specificity of antagonist and affinity influence are very little, and detection sensitivity can reach 0.5ng/ml, has reached the sensitivity similar to the ELISA method.
3) easy and simple to handle: the single stage method operation, detect EPSPS and BT-Cry1Ab/1Ac simultaneously, do not need other any auxiliary instrumentation equipment.Directly analyte sample fluid is dripped and in well, get final product.
4) testing result image, accurate, quick: testing result can directly be judged by naked eyes in 3-5 minute.Article two, the aubergine band is positive, and an aubergine band is negative.
5) economical and efficient: a detection box can detect 2 kinds of different transgenosis compositions simultaneously, greatly reduces use cost.
6) be widely used: blade, seed and the converted products that can detect all kinds of crops such as corn, cotton, paddy rice, tobacco.Because joint inspection kit provided by the invention can carry out EPSPS ﹠amp to plant sample simultaneously; BT-Cry1Ab/1Ac analyzes, detect quick, special, responsive, convenient, be specially adapted to the development of transgenic product and the rapid screening of transgenic product, can be used for frontier defense, customs's plant detection, food safety detection and seed and manage sales department scene, rapid screening testing sample.
Description of drawings:
Fig. 1～Fig. 3 is the structural representation of a kind of kit of the present invention.
Wherein Fig. 1 is the vertical view of base plate, and can see has two calibration tapes in the base plate;
Fig. 2 is the vertical view of kit loam cake, and 1 is well among Fig. 2, the 2nd, and observation window;
Fig. 3 is the structural representation of calibration tape film reaction system.
Fig. 4～Fig. 6 is a kit testing result synoptic diagram.Wherein Fig. 4 represents that testing result is invalid, and Fig. 5 represents negative findings, and Fig. 6 represents positive findings.T represents detection line among the figure, and C represents nature controlling line.
Embodiment:
Embodiment 1 preparation calibration tape
Below bring and illustrate with the test for preparing detection EPSPS:
1.EPSPS the preparation of odd contradictive hydroperitoneum
Mouse peritoneal injecting fluid paraffin 0.5ml.After 1 week, EPSPS monoclonal antibody hybridoma is injected in above BALB/C mice abdominal cavity of anticipating, every injected in mice hybridoma 1～3 * 106.Gather in the crops ascites after 10 days, above process is all finished under aseptic condition.
2.EPSPS Purification of Monoclonal Antibodies
1) with DEAE ion-exchange chromatography and Sephacryl S300 molecular sieve monoclonal antibody is carried out purifying; The SDS-PAGE disk electrophoresis detects the purity of purified monoclonal antibody; The ELISA method is measured the monoclonal antibody activity;
2) purge process: get mouse odd contradictive hydroperitoneum → 3,000rpm is centrifugal, and 15 minutes → supernatant adds 50% ammonium sulfate precipitation, spend the night → 3, it is centrifugal that centrifugal 20 minutes of 000rpm → precipitation is dissolved in 0.01M phosphate buffer (pH7.2) → S-300 gel column → DEAE Blue chromatographic column → 0-800mM NaCl gradient elution → ultrafiltration, concentrates monoclonal antibody;
3) monoclonal antibody purity testing: conventional SDS-PAGE electrophoresis, the purity of the monoclonal antibody that said method is purified are all more than 95%;
4) determination of protein concentration: ultraviolet spectrophotometer is measured the O.D. value at 279nm place, calculates protein content: OD as follows
279/1.4=mg/ml (purified monoclonal antibody).With the monoclonal antibody concentration adjustment is about 1mg/ml;
5) monoclonal antibody determination of activity: select the antigen coated elisa plate of EPSPS (1 μ g/ hole) for use, and sheep anti-mouse igg-HRP compound, measure each monoclonal antibody tire (greater than 1: 5000).
3. goat-anti DOA
THeight tire immune sero-fast preparation and purifying
1) above-mentioned monoclonal antibody+Freund's complete adjuvant that purifying is good → immune goat → booster immunization secondary each strengthens getting in back about 10 days blood → centrifuging and taking serum → ammonium sulfate precipitation → DEAE ion-exchange chromatography purifying January → second time at interval;
2) titration: the ELISA sandwich method, the antibody titer dilutability should be greater than 1: 256;
3) determination of protein concentration: ultraviolet spectrometry is measured OD
279, calculate protein concentration.
4. collaurum and golden labeling antibody preparation
1) preparation of collaurum: the collaurum for preparing 40-60nm with the citrate reducing process.With 0.01%HauCl
4Be heated to and boil, add a certain amount of 1% trisodium citrate (Na
3C
6H
5O
72H
2O), continued heated and boiled 5 minutes, treat the colloidal gold solution color, after stablizing, cool off and get final product by orchid → purple → red.Colloidal gold solution should be limpid transparent, as the need long preservation, can add 0.02%NaN
3
2) collaurum liquid 500ml is transferred pH8.4 with 0.1M NaOH, slowly add monoclonal antibody 2ml under the magnetic agitation, stirred 20 minutes.5, the centrifugal 30Min of 500rpm removes unconjugated protein in the supernatant.Colloid gold label antibody precipitation is drawn in centrifugal back, and precipitation is dissolved in 10ml and preserves in the liquid, with 0.45 μ m filtering with microporous membrane.Sampling is examined and determine, and all the other put 4 ℃ of preservations.
5. the preparation of film reaction system M1
1) sheep anti-mouse igg with EPSPS antibody and purifying dilutes with the 0.1M phosphate buffer, and final concentration is about 0.2-2mg/ml.
2) sheep anti-mouse igg of purifying is dissolved in the 0.1M phosphate buffer, and concentration is 2.0mg/ml;
3) nitrocellulose filter size: 10cm * 27cm, every film can spray the detection of long 25cm and control each 4 on band;
4) above two kinds of solution are added respectively in two shower nozzle storage bottle of Biodot XYZ3000 flush coater;
5) spray speed being set is 2 μ l/cm, and the speed of dividing a word with a hyphen at the end of a line of NC film is 50mm/s.Wrap on the NC film by the reaction detection line with monoclonal antibody EPSPAD2 (2mg/ml), reacted control line with 1.0mg/ml rabbit anti-mouse igg bag, the distance of control line and detection line is 4-4.5mm;
6) finish bag by after, put 37 ℃ of drying boxes 24 hours, handle half an hour with the sealing of BB confining liquid, take out with the WB washing lotion and wash once;
7) 37 ℃ of drying box drying for standby;
8) nitrocellulose filter got ready of cutting: 1.8cm * 27cm/ bar, put into the thin aluminum bag hermetically drying and preserve.It is standby to put room temperature preservation.
6. the preparation of film reaction system M2a, M2b and M3
The water-absorption fiber film is soaked in respectively in M2a, M2b, the M3 solution, and after soaking into, taking-up is dried, in the polybag of packing into, and room temperature preservation.
1) M2a preparation: the glass fibre that makes is cut 27cm * 1.2cm/ bar;
2) M2b preparation: the glass fibre that makes is cut 27cm * 1.8cm/ bar;
3) M3 preparation: the glass fibre that makes is cut 27cm * 1.2cm/ bar.
7. the preparation of film reaction system M4
1) get collaurum-monoclonal antibody compound and add dilution, mixing is made into working concentration;
2) solution is added in the Airjet shower nozzle storage bottle of flush coater Biodot;
3) set pressure is 15PSI, and the translational speed of glass fibre membrane is 50mm/s;
4) spray specification is 0.5-1.5cm * 25cm/ bar, and every sprays 2 times;
5) in 37 ℃ of oven dry 12 hours, put into thin aluminum bag, add drying agent, heat sealing, room temperature preservation.
8. reaction body assembling
1) on the M6 plastic base, pastes two of double sticky tape M7;
2) in the middle of M7, paste reaction film M1 (18mm), from the about 22mm of M6 upper limb;
3) on M7, paste M5 (22mm), align with the M6 upper limb and hand over 1mm with the M1 upper limb;
4) on M7, paste M2a (12mm), join with the M1 lower edge;
5) on M2, paste M4 (10mm), push down M1 lower edge 0.5mm;
6) paste M3 (12mm) on M7, upper limb is pushed down M4 about 2/3;
7) paste M2b (18mm) on M7, it is about 2/3 that upper limb is pushed down M4, and lower edge aligns with the lower edge of M7;
8) paste transparency protected adhesive tape M8 on M4 and M7, upper limb is pushed down M4 fully, and pushes down the about 1.5mm of M1;
9) paste colour-coded adhesive tape M9 on M5, hand over 1mm with the M1 upper limb, the other end climbs over the M6 upper limb, is affixed on the M6 quilt cover;
10) the full-automatic cutting cutter of reaction body and function with assembled formation is cut into the 3.5mm specification.
The assembling of embodiment 2 kits
The detection EPSPS albumen for preparing and two kinds of calibration tapes detecting BT albumen are embedded respectively in two grooves of base plate, then with loam cake and base plate fastening, kit.
With kit, dropper and the encapsulation of operation instructions dress polybag.
Embodiment 3 kit uses
1. detect sample process and preparation
Vegetable seeds, leaf, seedling equal samples need through grinding before detection, and with distilled water extracting and dilution.For obtaining the optimum detection effect, variant sample should after finishing grinding and dilution, with the sample mixing, leave standstill according to the dilution proportion in the following table, gets supernatant as test sample.
| Floristics | Leaf and seedling | Seed |
| Leaf/distilled water (g/ml) | Seed/distilled water (g/ml) | |
| Corn | 1∶20 | 1∶2 |
| Cotton | 1∶10 | 1∶4 |
| All the other dicotyledons | 1∶10 | 1∶4 |
| All the other monocotyledons | 1∶20 | 1∶2 |
2. detect and the result
To detect box and keep flat, and with joining plastic suction pipe absorption sample liquid, drip 4-5 and drip in the well 1 that detects box, absorbent material slowly moves sample to be checked, and the film reaction system is activated.No matter whether testing gene is present in the plant sample, and an aubergine band all can appear at (C) in the Quality Control district.The aubergine band that (C) manifested in the Quality Control district is to judge whether enough plant sample liquid is arranged, and whether normal chromatography process standard simultaneously also as the inner quality standard of reagent.
3. the result judges:
Whether each observation window detects a kind of gene to be checked and exists.Below be the example explanation to detect EPSPS:
1) as not containing specific EPSPS albumen in the sample, or its concentration is lower than detection sensitivity, colloidal gold antibody can not be fixed on the monoclonal antibody immunity that detects on the film in the band in the chromatography process, thereby an aubergine detection band can not appear in (T) in the test section, show negative (-) result, an aubergine band promptly only in Quality Control district (C), occurs.(see figure 5)
2) if when treating that the EPSPS protein concentration is higher than its detection threshold in the plant sample, antigen immunity gold with detect with on another monoclonal antibody combine, another aubergine detection band will also appear in (T) in the test section, show positive (+) result, an aubergine band promptly in Quality Control district (C) and detection zone, respectively occurs.Attention: the aubergine band in test section (T) can show the phenomenon of shade.But, in the observing time of regulation, no matter this colour band shade all should be judged to be positive findings.(see figure 6)
3) the aubergine band do not occur as Quality Control district (C), then testing result is invalid, shows the rotten damage of incorrect operating process or kit.(see figure 4)
Claims (4)
1. the kit of combined testing EPSPS and BT albumen, it comprises base plate and loam cake, base plate cover is formed kit after going into loam cake, it is characterized in that being placed with on the base plate two calibration tapes; On be stamped two observation windows and two wells.
2. the kit of combined testing EPSPS according to claim 1 and BT albumen is characterized in that the upper surface of described base plate is provided with two grooves, and every groove is embedded in a calibration tape.
3. the kit of combined testing EPSPS according to claim 1 and 2 and BT albumen is characterized in that described observation window is a rectangle.
4. the method for combined testing EPSPS and BT albumen is characterized in that detecting with the kit of claim 1 or 2 described combined testing EPSPS genes and BT gene; Operation steps is: the same a kind of extract that will inspect article by ready samples drips respectively in two wells of kit, after 3-5 minute, by two observation window Direct observation results of loam cake.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510086760 CN1773285A (en) | 2005-11-02 | 2005-11-02 | Reagent kit and method for combined testing EPSPS and BT protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510086760 CN1773285A (en) | 2005-11-02 | 2005-11-02 | Reagent kit and method for combined testing EPSPS and BT protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1773285A true CN1773285A (en) | 2006-05-17 |
Family
ID=36760352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510086760 Pending CN1773285A (en) | 2005-11-02 | 2005-11-02 | Reagent kit and method for combined testing EPSPS and BT protein |
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| Country | Link |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008104081A1 (en) * | 2007-03-01 | 2008-09-04 | Biolytical Laboratories Inc. | Parallel immunoassay device |
| CN110133279A (en) * | 2019-04-15 | 2019-08-16 | 上海市农业科学院 | A kind of joint inspection colloidal gold strip detecting transgenosis BT albumen and CP4-EPSPS albumen |
| CN112485437A (en) * | 2020-11-10 | 2021-03-12 | 基因赛奥(大连)生物科技发展有限公司 | CP4EPSPS and Bt Cry1Ab/Ac double-protein rapid test paper card and preparation and use methods thereof |
-
2005
- 2005-11-02 CN CN 200510086760 patent/CN1773285A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008104081A1 (en) * | 2007-03-01 | 2008-09-04 | Biolytical Laboratories Inc. | Parallel immunoassay device |
| CN110133279A (en) * | 2019-04-15 | 2019-08-16 | 上海市农业科学院 | A kind of joint inspection colloidal gold strip detecting transgenosis BT albumen and CP4-EPSPS albumen |
| CN112485437A (en) * | 2020-11-10 | 2021-03-12 | 基因赛奥(大连)生物科技发展有限公司 | CP4EPSPS and Bt Cry1Ab/Ac double-protein rapid test paper card and preparation and use methods thereof |
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