CN1769438A - Phytase uses and using method - Google Patents
Phytase uses and using method Download PDFInfo
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- CN1769438A CN1769438A CN 200510014732 CN200510014732A CN1769438A CN 1769438 A CN1769438 A CN 1769438A CN 200510014732 CN200510014732 CN 200510014732 CN 200510014732 A CN200510014732 A CN 200510014732A CN 1769438 A CN1769438 A CN 1769438A
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Abstract
The invention discloses the use of phytase as residual pesticide degrading agent, wherein the method of applying comprises, homogeneously sprinkling solution containing 0.1-100U/ml phytase onto the overall surface of the plants with residual pesticides, keeping for 6-24 hours, or immersing 10-60 minutes with solution containing 0.1-100U/ml phytase.
Description
Technical field
The claimed technical scheme of the present invention relates to a kind of lytic enzyme that acts on the ester bond, specifically a kind of purposes of phytase and using method.
Background technology
Agricultural products in China pesticide residue problem is more serious.Its reason is, the production and selling market of China's agricultural chemicals standard very not on the one hand, and some riskiest pesticides that should eliminate are still continuing production and selling; Also standard very not of the application of China's agricultural chemicals on the other hand, the producer uses riskiest pesticide or excess to use the phenomenon ubiquity of agricultural chemicals on vegetable and fruit in violation of rules and regulations, causes that pesticide residue exceed standard in many agricultural-food.
The existing technical products that is used to remove remains of pesticide has soapless soap class, ozonizer class and biologically digesting enzyme or degraded enzyme clean-out system.
Soapless soap class material also has stronger infringement to skin; Ozone is very harmful to human body, and some are easy to people hypersensitive, is exposed to the environment that ozone content surpasses 180 microgram/cubic meters for a long time, can produce skin urtication, unsmooth breath, cough and rhinitis symptom; Removing remains of pesticide with biologically digesting enzyme or degraded enzyme process is a biotechnology of new development in recent years, and its advantage is certain remains of pesticide removing effect, nontoxic, safe in utilization, environmentally degradable.Guyuan Bioengineering Co Ltd, Anhui has proposed a kind of organic phosphorous chemicals decomposing enzyme, its encoding gene and production method in CN1381574; Biological Technology institute, Chinese Academy of Agricultural Sciences and Institute of Feeds,China Academy of Agriculture Sciences have proposed a kind of new opd and encoding gene thereof in CN164468.These enzymes all are to clear up or degrading enzyme by the organophosphorus pesticide of the method preparation of genetic engineering means, microbial fermentation.But these biologically digesting enzymes or degrading enzyme suitability for industrialized production difficulty are big, manufacturing cost is high, so price is too high, is more than 20 times of common Betengent product price, and market is difficult to accept extensively; In addition, result through experimental study shows that the effect of " healthy custom " the board bio-enzyme cleaning agent that the remains of pesticide removing product Anhui Guyuan biotechnology limited liability company that adopts these technology to produce produces and the actual removing remains of pesticide of other these class bio-enzyme cleaning agents is general.
Phytase is a kind of existing biological substance, is widely used in animal-feed both at home and abroad.CN98105114.6, CN97197336.9, CN99103564.X, CN98807104.5, CN99804009.6, CN00805245.X, CN00134591.5, CN01107394.2, CN01107392.6, CN01107393.4, CN01107395.0, CN01142162.2, CN01127288.0, CN02136530.X, CN02136531.8, CN01820656.5, CN 200410004373.2, CN 200310108806.4, CN03137476.X, CN03151019.1, CN02827413.X, CN02828400.3, CN02828409.7, CN200310112910.0 has reported the preparation method of phytase and has been used for this unique purposes of animal-feed.Do not find that by literature search phytase has other purposes, do not have phytase to be used as the report of degrading pesticide residue agent yet.
Summary of the invention
Technical problem to be solved by this invention is: provide a kind of with brand-new purposes and the using method of phytase as the degrading pesticide residue agent.Phytase as the degrading pesticide residue agent remove that remains of pesticide is effective, safety non-toxic, easily degraded in environment, low production cost and easy to use.
The present invention solves this technical problem the technical scheme that is adopted:
Phytase is used as the degrading pesticide residue agent.
Phytase as the using method of degrading pesticide residue agent is, the solution that will contain phytase 0.1～100U/ml evenly is sprayed on the whole surface of the plant that contains remains of pesticide, kept 6～24 hours, the plant that maybe will contain remains of pesticide was with the solution soaking that contains phytase 0.1～100U/ml 10～60 minutes.
GB/T 18634-2002 is abideed by in the definition of phytase activity unit among the present invention " U/ml ".
Phytase is existing ready-made commodity, and has all bought easily various places.
The invention has the beneficial effects as follows: the present invention is a kind of brand-new purposes of phytase with phytase as the degrading pesticide residue agent, and the remains of pesticide of removing on the plant is had beyond thought outstanding effect, and detailed data is asked for an interview embodiment.
Phytase is the fodder additives of domestic and international widespread use, and by biological fermentation process production, it is nontoxic, security has obtained the authentication of the various countries Ministry of Health and application for over ten years proves, therefore degrading pesticide residue agent of the present invention has good security.
Phytase is a kind of protein in essence, and its biological degradability is very good, can not pollute environment, so the present invention is environmentally friendly as the degrading pesticide residue agent with phytase.
Because therefore phytase large-scale industrialization production and sales, cheap are used as the degrading pesticide residue agent with phytase, the cost that remains of pesticide is handled is low.
Phytase is handled the very easy to use of remains of pesticide as the degrading pesticide residue agent, as long as being mixed with solution on request, the phytase commodity of buying evenly are sprayed on the plant that the plant surface that contains remains of pesticide or immersion have remains of pesticide, through promptly reaching the ideal effect of removing remains of pesticide behind the certain hour.
Embodiment
Embodiment 1
Methidathion with 95% is made into the even Plantula Brassicae chinensis of spraying 3～8 leaves of solution of 0.01mg/ml, the phytase aqueous solution of getting the 0.1U/ml that " challenge " board phytase that its 1/3 usefulness Beijing challenge group produces is made in 24 hours later on sprays processing, all the blade face evenly squirts, got later the Plantula Brassicae chinensis sample of above different treatment respectively after 24 hours, wherein the Plantula Brassicae chinensis sample of handling without phytase is got half with the phytase solution soaking of same 0.1U/ml 60 minutes, according to GB/T5009.199-2003 " rapid detection of organophosphorus and carbamate pesticide residue amount in the vegetables " above Plantula Brassicae chinensis sample through three kinds of different treatment is detected, detecting instrument is a GDYN-101S type residual toxicity field quick detection instrument, measured inhibiting rate value (E value) the results are shown in Table 1.
Table 1 phytase solution is to the removing effect of methidathion
| Sequence number | The sample title | Sample pesticide post processing mode | The E value |
| 1 | Plantula Brassicae chinensis | The sampling of being untreated after 48 hours | 86％ |
| 2 | Plantula Brassicae chinensis | Spray the phytase liquid of 0.1U/ml after 24 hours, sampling after 24 hours | 32％ |
| 3 | Plantula Brassicae chinensis | Phytase liquid with 0.1U/ml after 48 hours soaks, sampling after 60 minutes | 38％ |
Embodiment 2
Rogor is made into the even naked oats dish that sprays 3～8 leaves of solution of 0.005mg/ml, getting the water phytase spray solution of the 100U/ml that phytase that its 1/3 usefulness produced by A/B company is made in 24 hours later on handles, all the blade face evenly squirts, got later the naked oats dish sample of above different treatment respectively after 12 hours, wherein half phytase solution with same 100U/ml of naked oats dish sample of handling without phytase all embathed 10 minutes, with GDYN-101S type residual toxicity field quick detection instrument above naked oats dish sample through three kinds of different treatment is detected, measured inhibiting rate value (E value) sees Table 2.
Table 2 phytase solution is to the removing effect of Rogor
| Sequence number | The sample title | Sample pesticide post processing mode | The E value |
| 1 | The naked oats dish | The sampling of being untreated after 36 hours | 98％ |
| 2 | The naked oats dish | Spray the phytase liquid of 100U/ml after 24 hours, sampling after 12 hours | 27％ |
| 3 | The naked oats dish | Phytase liquid with 100U/ml after 36 hours soaks, sampling after 10 minutes | 31％ |
Embodiment 3
The solution that Le Siben is made into 0.003mg/ml evenly sprays the parthenocissus leaf, the phytase aqueous solution of getting the 10U/ml that its 1/3 usefulness is made into by " enzyme he richness " phytase of BASF AG in 24 hours later on sprays to be handled, all the blade face evenly squirts, got later the parthenocissus leaf sample of above different treatment respectively after 6 hours, wherein the phytase solution without the same 10U/ml of parthenocissus leaf sample 1/2 usefulness of phytase processing all embathed 15 minutes, with GDYN-101S type residual toxicity field quick detection instrument above parthenocissus leaf sample through three kinds of different treatment is detected, measured inhibiting rate value (E value) sees Table 3.
Table 3 phytase solution is to the removing effect of Le Siben
| Sequence number | The sample title | Sample pesticide post processing mode | The E value |
| 1 | The parthenocissus leaf | The sampling of being untreated after 30 hours | 76％ |
| 2 | The parthenocissus leaf | Spray the phytase liquid of 10U/ml after 24 hours, sampling after 6 hours | 25％ |
| 3 | The parthenocissus leaf | Phytase liquid with 10U/ml after 30 hours soaks, sampling after 15 minutes | 34％ |
Embodiment 4
Methidathion with 95% is made into the even Plantula Brassicae chinensis of spraying 3～8 leaves of solution of 0.01mg/ml, divided 4 parts later in 24 hours, wherein do not do aftertreatment for 1 part, 3 parts of phytase aqueous solution soaking of using the 1U/ml that " Danisco " board phytase is made into respectively in addition, 0.5% commercially available " opening rice " board fruits and vegetables clean-out system soaks, 0.2% commercially available " healthy custom " board fruits and vegetables clean-out system soaked 30 minutes, with GDYN-101S type residual toxicity field quick detection instrument above a kind of Plantula Brassicae chinensis samples unprocessed and four kinds of process different treatment are detected, measured inhibiting rate value (E value) sees Table 4.
Above-mentioned " opening rice " board fruits and vegetables clean-out system lotion is pure surfactant-based washing composition, and the effective constituent of " healthy custom " board fruits and vegetables clean-out system is the organophosphorus zymolase.
Table 4 phytase solution is to the removing effect of methidathion
| Sequence number | The sample title | Sample pesticide post processing mode | The E value |
| 1 | Plantula Brassicae chinensis | The sampling of being untreated after 24 hours | 78％ |
| 2 | Plantula Brassicae chinensis | Soak sampling after 30 minutes after 24 hours with the 1U/ml phytase | 26％ |
| 3 | Plantula Brassicae chinensis | Soak sampling after 30 minutes after 24 hours with 0.5% " opening rice " | 32％ |
| 4 | Plantula Brassicae chinensis | Soak sampling after 30 minutes with 0.5% " healthy custom " after 24 hours | 41％ |
Claims (2)
1. the purposes of a phytase is characterized in that: phytase is used as the degrading pesticide residue agent.
2. the using method of the purposes of the described a kind of phytase of claim 1, it is characterized in that: the solution that will contain phytase 0.1～100U/ml evenly is sprayed on the whole surface of the plant that contains remains of pesticide, kept 6～24 hours, the plant that maybe will contain remains of pesticide was with the solution soaking that contains phytase 0.1～100U/ml 10～60 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200510014732 CN1769438A (en) | 2005-08-10 | 2005-08-10 | Phytase uses and using method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510014732 CN1769438A (en) | 2005-08-10 | 2005-08-10 | Phytase uses and using method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101648056B (en) * | 2009-09-23 | 2011-02-09 | 邓传安 | Pesticide residue scavenging agent |
| CN103911235A (en) * | 2014-03-25 | 2014-07-09 | 甘肃农业大学 | Effervescent tablet for removing pesticide residue |
| CN108850785A (en) * | 2018-06-27 | 2018-11-23 | 罗瑞格斯（广东）生物科技有限公司 | A kind of Methods of Degrading Pesticide Residues for vegetable and fruit |
-
2005
- 2005-08-10 CN CN 200510014732 patent/CN1769438A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101648056B (en) * | 2009-09-23 | 2011-02-09 | 邓传安 | Pesticide residue scavenging agent |
| CN103911235A (en) * | 2014-03-25 | 2014-07-09 | 甘肃农业大学 | Effervescent tablet for removing pesticide residue |
| CN103911235B (en) * | 2014-03-25 | 2017-02-01 | 甘肃农业大学 | Effervescent tablet for removing pesticide residue |
| CN108850785A (en) * | 2018-06-27 | 2018-11-23 | 罗瑞格斯（广东）生物科技有限公司 | A kind of Methods of Degrading Pesticide Residues for vegetable and fruit |
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