CN1742564A - Method for breeding group seedling-culture of peony and method for preventing it from browning and vetrificating - Google Patents
Method for breeding group seedling-culture of peony and method for preventing it from browning and vetrificating Download PDFInfo
- Publication number
- CN1742564A CN1742564A CN 200510086591 CN200510086591A CN1742564A CN 1742564 A CN1742564 A CN 1742564A CN 200510086591 CN200510086591 CN 200510086591 CN 200510086591 A CN200510086591 A CN 200510086591A CN 1742564 A CN1742564 A CN 1742564A
- Authority
- CN
- China
- Prior art keywords
- medium
- brownization
- culture
- vitrifying
- tree peony
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention uses the dormand bud to be activated in early spring as explant and adopts the following steps: sterilizing, removing bud scale, cutting off small leaflet in the bud, inoculating residual portion on the different culture media to make culture, then regularly making subculture so as to implement propagation of lots of tissue-cultured seedlings and prevent the production of browning and vitrifiation.
Description
Technical field
The present invention relates to the plant seedling propagation technique, relate in particular to a kind of tree peony tissue cultivating seedling propagation and brownization and vitrifying and prevent method.
Background technology
Tree peony is traditional famous flower of China's special product, and it is Chinese nation's prosperity, flourishing, peaceful happy symbol that China people always look tree peony.In recent years, tree peony beautify the environment, application aspect the commodity production, Social Culture more and more widely, this need be based on a large amount of tree peony nursery stocks, and tree peony has only the single-lobe kind solid many, half double variety takes second place, double variety is generally shaky, and the seedling variation is big, is difficult for keeping the maternal plant merit.In addition, traditional modes of reproduction such as grafting, plant division and the two flat method that occurred are in recent years bred, and also all need original propagating materialss such as a large amount of stocks, scion, plant, so reproduction speed are also unhappy, can't satisfy needs of society.
Tissue culture is one of effective means of micropropagation of plants, and a large amount of choiceness nursery stock and propagating materialss can be provided in a short time.Recent two decades comes, and domestic and international many scholars are devoted to the research of tree peony tissue culture.With the bud is in the tree peony group training report of explant, generally believes that the sleeping bud that is about to sprout period in early spring is a best material; The MS minimal medium of MS or improvement is the suitable culture base; Hormone BAP and GA
3Being used can well-grown and obtain the higher rate of increase; Calcium ion concentration doubles preventing the explant vitrifying, and it is useful to improve effective rate of increase.Because numerous interracial the differing greatly of kind of tree peony, different kinds need be treated respectively during the group training, has more aggravated the difficulty of group training.Problems such as in tree peony group training, it is low that various kinds generally show reproduction coefficient in a word, and brownization, vitrifying are serious.It is reported that the rate of increase of various kinds is generally lower in the training of tree peony group, generally about 3.0, that is to say that the rate of increase is still waiting further raising; Simultaneously, the vitrifying of tissue cultivating seedling causes deformity, the morbid state of tissue cultivating seedling, has reduced the quality and the rate of increase of seedling; And the generation of brownization produces murder by poisoning to tissue cultivating seedling, causes death, has also reduced the rate of increase of tissue cultivating seedling.Therefore, around improve the rate of increase and how to prevent that the problem of vitrifying and brownization from being the bottleneck of tree peony tissue culture technology development always.Only resolve them, just can break through this technical barrier of tree peony tissue culture.
Summary of the invention
(1), the technical problem that will solve
The object of the present invention is to provide a kind of tree peony tissue cultivating seedling propagation and brownization and vitrifying to prevent method.
(2), technical scheme
The present invention prevents method for a kind of tree peony tissue cultivating seedling propagation, brownization and vitrifying, and used medium is:
Minimal medium+BAP 0.5-1.0mg/L+GA
30.2-1.0mg/L.
Medium of the present invention, described minimal medium is MS medium, Ca when wherein being used to breed
2+Double.
Medium of the present invention, described minimal medium is a modified MS medium when being used to prevent brownization.
Medium of the present invention, described modified MS medium is 1/2～1/3 trace element, Ca when wherein being used to prevent brownization
2+Double CaCl
2With Ca (NO
3)
2Mol ratio be 1: 1.
Medium of the present invention, described minimal medium is a modified MS medium when wherein being used to prevent vitrifying.
Medium of the present invention, described modified MS medium is 1/2 macroelement, Ca when wherein preventing vitrifying
2+Double CaCl
2With Ca (NO
3)
2Mol ratio be 1: 1.
(3), beneficial effect
Pass through screening in the test to hormone concentration, obtained the best hormone concentration of suitable different cultivars propagation, generally improved the rate of increase of these kinds, especially for gilvous, the pleasant kind ' high noon ' of fragrance, its rate of increase is brought up to 8.00, advanced in years again going a step further on the tree peony tissue culture technology.
Studies show that, adjusted the consumption of some elements in the MS minimal medium, discovery has tangible effect to vitrifying and the browning that alleviates the tree peony tissue cultivating seedling, containing on the improvement MS minimal medium of 1/2 macroelement, the vitrifying seedling ratio of ' WULONGPENGSHANG ' and ' star ' two kinds is all minimum, and especially the latter does not have the appearance of vitrifying seedling fully, improved the quality and the actual rate of increase of tree peony tissue cultivating seedling greatly, provide assurance for overcoming a tree peony group training difficult problem as early as possible.
Thereby variation that it is generally acknowledged trace element participation enzymic activity influences brownization of plant tissue, and discovery of the present invention is compared with trace element, and macroelement is bigger to the influence of brownization; Macroelement reduces, and brownization increases the weight of, and supposition is because suppressed the growth of tissue cultivating seedling, thereby has influenced the physiological function of inside plants.On modified MS medium, the brownization situation of ' WULONGPENGSHANG ', 93% shows as 1 grade, and ' star ' 83% shows as 1～2 grade, and be all lighter, can think brownization do not take place.Content of elements also influences brownization, ' WULONGPENGSHANG ' and ' star ' respectively on the medium that contains 1/2 and 1/3 trace element brownization the lightest.Alleviating of brownization reduced the injury of noxious material to tissue cultivating seedling, for normal, the healthy growth of tissue cultivating seedling provides strong assurance.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 propagation
Medium: with MS (Ca
2+Double) be minimal medium, sucrose 40g/L, agar 6g/L, pH value 5.8, additional hormone is:
' high noon ': BAP1.0mg/L, GA
30.2mg/L;
' WULONGPENGSHANG ': BAP0.5mg/L, GA
30.5mg/L;
' star ': BAP0.5mg/L, GA
31.0mg/L;
' Corallium Japonicum Kishinouye platform ': BAP1.0mg/L, GA
30.5mg/L;
' purple two Qiao ' BAP1.0mg/L, GA
30.5mg/L.
With the sleeping bud that is about to sprout early spring is explant, peels off perula behind the sterilization, cuts away bud endite sheet, remainder is seeded on the medium cultivates, and observes statistics once, and writes down the rate of increase and the upgrowth situation of various kinds on different medium in per 7 days.Carry out subculture after cultivating for 5 weeks, method is to cut blade from the tissue cultivating seedling petiole base, again along the axis rip cutting of stem, 0.5 centimeters crosscut below the position that has sprouting to take place, the part that obtains is seeded in cultivation (later subculture process is identical) on the new medium, carry out successive transfer culture altogether 3 times, observe the same initial culture of statistical method.Condition of culture is 25 ℃ of room temperatures, lighting delay number 14h/d, light intensity 2000Lx.
Table 1 tree peony tissue cultivating seedling growing state
| Kind | Stem height (cm) * | The rate of increase ** |
| ' high noon ' | 1.28 | 8.00 |
| ' WULONGPENGSHANG ' | 1.27 | 3.20 |
| ' star ' | 0.89 | 4.23 |
| ' Corallium Japonicum Kishinouye platform ' | 1.40 | 5.61 |
| ' purple two Qiao ' | 1.09 | 4.20 |
* listed stem height is the mean value of 3 subculture processes in the table.
The listed rate of increase is the mean value of the II time successive transfer culture in the * table, and the rate of increase is in certain cultivation period, total bud number that breaks up on the explant and the ratio of inoculating the explant sum.
Embodiment 2 prevents vitrifying
Constant (the Ca of content that keeps other element of MS minimal medium
2+Double), 1/2 times of macroelement (CaCl
2With Ca (NO
3)
2Mol ratio be 1: 1), sucrose 40g/L, agar 6g/L, PH5.8, the hormone concentration of each kind adopts the concentration among the embodiment 1.
With the sleeping bud that is about to sprout early spring is explant, peels off perula behind the sterilization, cuts away bud endite sheet, remainder is seeded on the medium cultivates.Carry out subculture after cultivating for 5 weeks, method is to cut blade from the tissue cultivating seedling petiole base, again along the axis rip cutting of stem, and 0.5 centimeters crosscut below the position that has sprouting to take place, the part that obtains is seeded in cultivation (later subculture process is identical) on the new medium, carries out successive transfer culture altogether 2 times.The observed and recorded experimental result.Condition of culture is 25 ℃ of room temperatures, lighting delay number 14h/d, light intensity 1800Lx.
Table 2 medium compares the significance of ' WULONGPENGSHANG ' and ' star ' vitrifying seedling ratio influence
| Medium | Vitrifying seedling ratio (%) * | Significance relatively | |
| a＝0.05 | A＝0.01 | ||
| ' WULONGPENGSHANG ' | 14.3 | a | A |
| ' star ' | 0 | b | B |
*Vitrifying seedling ratio listed in the table is the mean value of 2 subculture processes.
Embodiment 3 prevents brownization,
The hormone concentration of each kind adopts the concentration of embodiment 1, sucrose 40g/L, agar 6g/L, PH5.8.
' WULONGPENGSHANG ': modified MS medium, 1/2 trace element, Ca
2+Double CaCl
2With Ca (NO
3)
2Mol ratio be 1: 1.
' star ': modified MS medium, 1/3 trace element, Ca
2+Double CaCl
2With Ca (NO
3)
2Mol ratio be 1: 1.
With the sleeping bud that is about to sprout period in early spring is explant, peels off perula behind the sterilization, cuts away bud endite sheet, remainder is seeded on the medium cultivates, the brownization rank of the per 7 days various kinds of observed and recorded on different medium.Carry out subculture after cultivating for 5 weeks, method is to cut blade from the tissue cultivating seedling petiole base, along the axis rip cutting of stem, 0.5 centimeters crosscut below the position that has sprouting to take place is seeded in cultivation (later subculture process is identical) on the new medium with the part that obtains again.Carry out successive transfer culture altogether 3 times, observe the same initial culture of statistical method.
Condition of culture is 25 ℃ of room temperatures, lighting delay number 14h/d, light intensity 1600Lx.
Result's (mean values of 3 subculture processes): ' WULONGPENGSHANG ' 50% is 1～2 grade, and 38% is 4～5 grades; ' star ' 78% is 1～2 grade, and 8.7% more than 4 grades.
Illustrate: other division of brownization level: 1 grade, not brownization or brownization not substantially; 2 grades: brownization very slight color, brownization scope are around the explant in 0.3 centimetre; 3 grades: brownization is of light color, and brownization scope is around the explant in 0.4 centimetre; 4 grades: brownization color is placed in the middle, and brownization scope is around the explant in 0.5 centimetre; 5 grades: brownization color is dark, and brownization scope is about 0.5 centimetre on every side of explant; 6 grades: brownization color is the darkest, and brownization scope is around the explant more than 0.5 centimetre.
Claims (6)
1, a kind of tree peony tissue cultivating seedling propagation, brownization and vitrifying prevent method, it is characterized in that medium is:
Minimal medium+BAP 0.5-1.0mg/L+GA
30.2-1.0mg/L.
2, medium as claimed in claim 1, described minimal medium is MS medium, Ca when it is characterized in that being used to breed
2+Double.
3, medium as claimed in claim 1, described minimal medium is a modified MS medium when it is characterized in that being used to prevent brownization.
4, medium as claimed in claim 3 is characterized in that described modified MS medium is 1/2～1/3 trace element, Ca
2+Double CaCl
2With Ca (NO
3)
2Mol ratio be 1: 1.
5, medium as claimed in claim 1, described minimal medium is a modified MS medium when it is characterized in that being used to prevent vitrifying.
6, medium as claimed in claim 5 is characterized in that described modified MS medium is 1/2 macroelement, Ca
2+Double CaCl
2With Ca (NO
3)
2Mol ratio be 1: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510086591XA CN100336445C (en) | 2005-10-12 | 2005-10-12 | Method for breeding group seedling-culture of peony and method for preventing it from browning and vetrificating |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510086591XA CN100336445C (en) | 2005-10-12 | 2005-10-12 | Method for breeding group seedling-culture of peony and method for preventing it from browning and vetrificating |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1742564A true CN1742564A (en) | 2006-03-08 |
| CN100336445C CN100336445C (en) | 2007-09-12 |
Family
ID=36138214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200510086591XA Expired - Fee Related CN100336445C (en) | 2005-10-12 | 2005-10-12 | Method for breeding group seedling-culture of peony and method for preventing it from browning and vetrificating |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100336445C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120128A (en) * | 2013-02-28 | 2013-05-29 | 河南科技大学 | Method for avoiding paeonia suffruticosa tissue culture seedling browning and vitrifying |
| CN104255490A (en) * | 2014-09-16 | 2015-01-07 | 郎溪庆林生态特色农业观光园有限公司 | Peony tissue culture and rapid breeding method |
| CN106212289A (en) * | 2016-08-26 | 2016-12-14 | 北京林业大学 | A kind of method setting up oil Paeonia suffruticosa bulbil efficient aseptic culture system |
| CN108094197A (en) * | 2017-11-30 | 2018-06-01 | 安徽心缘康生物科技有限公司 | A kind of oil tree peony phoenix pellet asexual multiplication seedling method |
| CN116602212A (en) * | 2023-06-06 | 2023-08-18 | 北京市农林科学院 | Paeonia plant in-vitro culture and proliferation method and special culture medium |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1600082A (en) * | 2004-09-30 | 2005-03-30 | 北京林业大学 | Tissue culture technique for peony |
-
2005
- 2005-10-12 CN CNB200510086591XA patent/CN100336445C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120128A (en) * | 2013-02-28 | 2013-05-29 | 河南科技大学 | Method for avoiding paeonia suffruticosa tissue culture seedling browning and vitrifying |
| CN104255490A (en) * | 2014-09-16 | 2015-01-07 | 郎溪庆林生态特色农业观光园有限公司 | Peony tissue culture and rapid breeding method |
| CN106212289A (en) * | 2016-08-26 | 2016-12-14 | 北京林业大学 | A kind of method setting up oil Paeonia suffruticosa bulbil efficient aseptic culture system |
| CN108094197A (en) * | 2017-11-30 | 2018-06-01 | 安徽心缘康生物科技有限公司 | A kind of oil tree peony phoenix pellet asexual multiplication seedling method |
| CN116602212A (en) * | 2023-06-06 | 2023-08-18 | 北京市农林科学院 | Paeonia plant in-vitro culture and proliferation method and special culture medium |
| CN116602212B (en) * | 2023-06-06 | 2024-05-03 | 北京市农林科学院 | Paeonia plant in-vitro culture and proliferation method and special culture medium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100336445C (en) | 2007-09-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109006475B (en) | Kiwi fruit micro-grafting method and kiwi fruit seedling raising method | |
| CN103238524B (en) | Method for rapidly breeding fritillaria pallidiflora by using tissue culture technique | |
| CN103444304B (en) | A kind of method impelled miracle fruit seed germination and improve survival rate of seedling | |
| CN108293878B (en) | Tissue culture seedling raising method for trichosanthes kirilowii Maxim tender leaves | |
| CN100336445C (en) | Method for breeding group seedling-culture of peony and method for preventing it from browning and vetrificating | |
| CN101926287A (en) | Method for culturing tissue of 'Zhongzhen No.1' | |
| CN103430845A (en) | Strawberry tissue culturing method | |
| CN111657151A (en) | Rapid seedling method for acer truncatum | |
| KR20110113918A (en) | Method for plant formation of blueberry through stem tip culture | |
| CN102960249A (en) | In-vitro efficient seedling cultivation method synchronous in rooting and growing by utilizing tender stem segments of thuja koraiensis | |
| CN103109728B (en) | Rapid seedling culturing method of pinus sylvestris in tube | |
| CN1586166A (en) | Quick breeding method for cymbidium hyridus high quality sprout | |
| CN1631102A (en) | Pleione test tube breeding ball production technique | |
| CN106613933A (en) | Method for inducing rooting of apple dwarf rootstock tissue culture seedling | |
| Ubalua et al. | The role of supporting substrates in ex vitro acclimatization and growth of tissue cultured cassava plantlets | |
| CN112772416B (en) | Tissue culture seedling raising method for phyllostachys microphylla | |
| CN1422526A (en) | Test tube quick-breeding method for konjak | |
| CN109744142A (en) | A kind of santal tissue-culturing quick-propagation method for culturing seedlings | |
| CN1255023C (en) | Quick breeding technolgy for Renanthera imschootiana Rolfe | |
| KR101582972B1 (en) | Raw Material Medium Composition for Culturing Mushroom and Method for Culturing Mushroom Using the Same | |
| CN1526278A (en) | Tissue culture breeding method for clonic fibraurea stem | |
| CN112106658B (en) | Tissue culture and rapid propagation method for gelsemium elegans | |
| CN1319437C (en) | Group culture medium of Spotleaf-orchis and rapid breeding method thereof | |
| CN112715368B (en) | Cornus walteri stem induced plant regeneration culture medium and tissue culture and rapid propagation method thereof | |
| CN112913693B (en) | Effective method for preventing plant callus browning and jelly formation by using bridging technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070912 Termination date: 20101012 |