CN1740773A - Double-light path seawater phosphate optical analyzing system - Google Patents
Double-light path seawater phosphate optical analyzing system Download PDFInfo
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- CN1740773A CN1740773A CN 200510037101 CN200510037101A CN1740773A CN 1740773 A CN1740773 A CN 1740773A CN 200510037101 CN200510037101 CN 200510037101 CN 200510037101 A CN200510037101 A CN 200510037101A CN 1740773 A CN1740773 A CN 1740773A
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Abstract
The invention provides a kind of highly sensitive double-light path seawater phosphate optical analyzing system, be a kind of device of measuring the seawater low phosphorus.By light source, two optical fiber water sample wares, peristaltic pump, spectrometer and computing machine constitute, the light input interface of two optical fiber water sample wares is connected with the light source delivery outlet by optical fiber respectively, peristaltic pump is connected to the liquid input interface of two optical fiber water sample wares, and the input end of spectrometer is connected with the light output interface of two optical fiber water sample wares respectively by optical fiber, and computing machine is connected with spectrometer by data line 8.The present invention is with " the water sample ware " of optical fiber as the phosphate analytical instrument, (the measurement light path as optical fiber water sample ware can reach to 5 meters can to become hundred times of ground to increase the measurement light path, be 50～100 times of present phosphate analytical instrument), thereby can become hundred times of ground lowering apparatus to detect phosphatic concentration limit, increase the measurement sensitivity of instrument, reach the phosphatic purpose of accurate analysis low concentration phosphate concn.
Description
Technical field
The present invention relates to a kind of device with optical method for measuring seawater low phosphorus.
Background technology
Phosphorus (PO
4-P) be to form the plasmic important element of biological cell, on thalassography, phosphorus and nitrogen, these three kinds of very important elements of silicon are called " source of students key element " or " biological restriction element ".Phosphorus is one of necessary nutritive salt of sea life, participates in the whole process of sea life vital movement, plays a significant role in marine ecosystems.The N/P ratio that it has been generally acknowledged that halonereid and oceanic water is general constant in 16: 1, and this ratio is called the Redfield ratio.When N/P ratio depart from Redfield than the time, may cause that the growth of phytoplankton is subjected to the restriction of the lower element of relative content.For vertebrate, phosphorus is the principal ingredient that constitutes its bone.Therefore the phosphorus in the seawater is one of controlling factor of marine animal and plant turnout.Accurately measure the content of phosphorus in the seawater, study its changes in distribution rule, not only very important to thalassochemistry, biological chemistry and the geochemical behavior of phosphorus in the research seawater, and to the marine ecology process study, the exploitation marine living resources are of great immediate significance.
Since be subjected to the restriction of measuring technique level, very difficult to the accurate measurement of low phosphorus, become one of difficult problem of current thalassochemistry circle.For example adopt present instrument that China's Nansha waters water sample phosphorus content is analyzed, in upper water, can't measure the content (phosphorus concentration is lower than detection lower limit 0.6 μ g/L) of phosphorus.The upper strata seawater is the most frequent layer position of most important vital movement in the marine ecosystem, owing to lack the definite data of phosphorus this " biological restriction element ", has had a strong impact on the further investigation to the marine superstructure ecological process.Critical role and the effect of ocean in global ecological environment changes obtained universally acknowledgedly, and the research of ocean and marine ecosystems is more and more come into one's own.Be not only China, countries in the world Marine Sciences circle press for method and the instrument and equipment of surveying low phosphorus, for scientific research of seas provides reliable observing data.
The method that phosphorus is measured in lab analysis is a lot, as the phosphorus molybdenum blue method, and organic solvent extractionprocess, chromatography, fluorescence method, magnesium hydroxide coprecipitation or the like.Wherein most widely used is the phosphorus molybdenum blue method.
The full name of phosphorus molybdenum blue method is " the phosphorus molybdenum blue method of ascorbic acid reduction ", its principle is to add sulfuric acid-ammonium molybdate-potassium antimonyl tartrate mix reagent and ascorbic acid solution in water sample, phosphate radical in the water sample and ammonium molybdate form phosphorus molybdenum Huang, in the presence of potassium antimonyl tartrate, phosphorus molybdenum Huang is reduced to the phosphorus molybdenum blue by ascorbic acid, utilizes spectrophotometer or special nutritive salt to analyze the concentration that instrument (as Skalar nutritive salt automatic analyzer) is measured phosphorus again.
Generally have only 5 to 10 centimetres owing to place the cuvette of water sample in the spectrophotometer, promptly measure light path and have only 5～10 centimetres, when the phosphorus concentration in the water sample is very low, the phosphorus molybdenum blue that water sample produces seldom, its absorption to light is very limited, therefore, the phosphorus concentration lower limit that can detect with spectrophotometer can not satisfy the measurement to low phosphorus than higher.
In order to solve the problem analysis of low phosphorus, the method of measuring low phosphorus is being explored in many in the world laboratories, scientists as Plymouth, Britain university Environmental Studies center is obtaining breakthrough aspect the detection low phosphorus, phosphorus (the Eric Achterberg that can lowest detection goes out 0.03 micrograms per litre, 2003, person-to-person communication), than low 20 times of standard method, but its detection method is subjected to the interference of salinity, and is inaccurate greater than 15 ‰ o'clock measurement results in salinity.Because the ocean seawater salinity of low phosphorus concentration surpasses 30 ‰, so the low-phosphorous measuring technique of Britain, also lacks practicality.The method that the employing chemistry that also has concentrates, the concentration that concentrates phosphorus earlier reaches the scope that existing instrument can detect, and then is converted into actual concentrations according to the volume of water sample before concentrating, and this method is more loaded down with trivial details, has increased the sample collecting amount simultaneously.
Summary of the invention
The objective of the invention is to propose a kind of double-light path seawater phosphate optical analyzing system that can improve greatly to the low phosphorus detection sensitivity.
According to the principle of material concentration being carried out analysis to measure with optical means---the absorptance of certain material that youth's primary-Beer law, photometer measurement obtain is not only relevant with the concentration of this material, and is also relevant with measurement light path (being the operating distance of light and this material).When the measurement light path was certain, big more then its absorptance of material concentration was also big more, and the two has linear relationship.On the other hand, if the concentration of material is certain, measuring the long more absorptance that records of light path also will be big more.Therefore, increase and measure light path, can increase the detectability of instrument low concentration.Double-light path seawater phosphate optical analyzing system of the present invention promptly is based on this principle and designs, and technical scheme of the present invention is that designed system includes:
One light source has the stable white light of light source delivery outlet output;
Two optical fiber water sample wares, each optical fiber water sample ware is by a suitable length, can inject liquid in it, the optical fiber that is installed in the housing in the coiling mode forms the water sample ware, one end of described water sample ware optical fiber has a smooth input interface and a liquid input interface, the other end of water sample ware optical fiber has a smooth output interface and a liquid output interface, and the light input interface of two optical fiber water sample wares is connected with the light source delivery outlet by optical fiber respectively;
One spectrometer is connected by the light output interface of optical fiber with two optical fiber water sample wares, measures the spectrum numerical value of liquid in two optical fiber water sample wares respectively;
One peristaltic pump is connected the liquid input interface of two optical fiber water sample wares, in order to inject test seawater sample and blank with reference to water respectively to two optical fiber water sample wares.
One computing machine is connected with spectrometer by data line, and the spectrum test interface is provided, the recorded and stored spectrum measuring data.
Along with development of fiber technology, succeeded in developing the optical fiber that can inject solution, can be made into multiple optical fiber water sample ware thus.The existing off-the-shelf of optical fiber water sample ware in the native system can be bought, as the LPC series optical fiber water sample ware by U.S. ocean (Ocean Optics) corporate agent.
Spectrometer in the native system can be set to two spectrometers, promptly two spectrum are provided with complete identical spectra instrument, the input end of two spectrometers is connected with the light output interface of two optical fiber water sample wares respectively, can measure the spectrum numerical value of liquid in two optical fiber water sample wares so simultaneously respectively, because one of them optical fiber water sample ware is to inject the test seawater sample, another optical fiber water sample ware is to inject blank with reference to water (being high purity water), thereby system can measure the ratio of the light intensity of sample and blank high purity water simultaneously, also be the absorptance of sample, thereby improve efficiency of measurement.
Method with systematic survey seawater phosphate of the present invention is as follows:
Early stage, sample treatment was with use spectrophotometer measurement phosphate method (phosphorus molybdenum blue method) is identical at present.Set up working curve earlier: dispose the sample of a series of known phosphorus concentrations, with peristaltic pump blank high purity water is injected an optical fiber water sample ware respectively, sample injects another water sample ware, the light intensity behind measurement and recording light source process sample and the blank high purity water; After measuring a sample, discharge sample water, inject next sample (blank high purity water needn't be changed at every turn), again measured light intensity; Successively all samples measurement is finished.The ratio of sample that measures and high purity water light intensity is called the absorptance of sample, set up the absorptance and the relation between the phosphorus concentration (promptly common alleged working curve) of the series of samples of known phosphorus concentration, absorptance of determining according to this working curve and the corresponding relation between the concentration are measured the absorptance of other unknown concentration sample again and are calculated phosphorus concentration.
Double-light path seawater phosphate optical analyzing system of the present invention is because with " the water sample ware " of optical fiber as the phosphate analytical instrument, (the measurement light path as optical fiber water sample ware can reach to 5 meters can to become hundred times of ground to increase the measurement light path, be 50～100 times of present phosphate analytical instrument), thereby can become hundred times of ground lowering apparatus to detect phosphatic concentration limit, increase the measurement sensitivity of instrument, reach the phosphatic purpose of accurate analysis low concentration phosphate concn.
Description of drawings
Fig. 1 is the structural representation of this double-light path seawater phosphate optical analyzing system;
Fig. 2 is the structural representation of the optical fiber water sample ware of this double-light path seawater phosphate optical analyzing system;
Fig. 3 is the observed result of this double-light path seawater phosphate optical analyzing system embodiment to phosphate standard model absorption coefficient.
Embodiment
Embodiment one
The structure of this double-light path seawater phosphate optical analyzing system embodiment as shown in Figure 1.
The optical fiber water sample ware of this double-light path seawater phosphate optical analyzing system embodiment has 1 meter light path, utilize native system to carry out the test of perphosphate measurement of concetration, 2 groups of phosphate samples have been prepared, concentration is 0.08,0.4,0.8,1.2,1.6 micromoles per liter, the absorption coefficient that obtains phosphate concn and 720nm place has good correlativity (as shown in Figure 3).Thereby, just can calculate the phosphate concn of measuring samples by the absorption coefficient of measuring samples according to working curve shown in Figure 3.
Embodiment two
The system architecture of this double-light path seawater phosphate optical analyzing system embodiment and embodiment one basic identical (as shown in Figure 1), difference is that the model that two optical fiber water sample wares all adopt U.S. ocean (Ocean Optics) corporate agent to sell is the optical fiber water sample ware of LPC-5 (5 meters light paths).
Claims (2)
1. double-light path seawater phosphate optical analyzing system is characterized in that comprising:
One light source has the stable white light of light source delivery outlet output;
Two optical fiber water sample wares, each optical fiber water sample ware is by a suitable length, can inject liquid in it, the optical fiber that is installed in the housing in the coiling mode forms the water sample ware, one end of described water sample ware optical fiber has a smooth input interface and a liquid input interface, the other end of water sample ware optical fiber has a smooth output interface and a liquid output interface, and the light input interface of two optical fiber water sample wares is connected with the light source delivery outlet by optical fiber respectively;
One spectrometer is connected by the light output interface of optical fiber with two optical fiber water sample wares, measures the spectrum numerical value of liquid in two optical fiber water sample wares respectively;
One peristaltic pump is connected the liquid input interface of two optical fiber water sample wares, in order to inject test seawater sample and blank with reference to water respectively to two optical fiber water sample wares.
One computing machine is connected with spectrometer by data line, and the spectrum test interface is provided, the recorded and stored spectrum measuring data.
2. double-light path seawater phosphate optical analyzing system according to claim 1, it is characterized in that described spectrometer is two spectrometers, promptly two spectrum are provided with complete identical spectra instrument, the input end of two spectrometers light output interface of two optical fiber water sample wares respectively connects, and measures the spectrum numerical value of liquid in two optical fiber water sample wares simultaneously respectively.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539358A (en) * | 2011-12-31 | 2012-07-04 | 燕山大学 | Real-time detection system of heavy metal in seawater |
| CN108918472A (en) * | 2018-08-27 | 2018-11-30 | 长春理工大学 | A kind of seawater pond calibration system based on seawater transmitance |
| CN110656185A (en) * | 2019-10-30 | 2020-01-07 | 浙江省医学科学院 | Primer group for detecting trichina gene through loop-mediated isothermal amplification, application and method |
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2005
- 2005-09-09 CN CN 200510037101 patent/CN1740773A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539358A (en) * | 2011-12-31 | 2012-07-04 | 燕山大学 | Real-time detection system of heavy metal in seawater |
| CN108918472A (en) * | 2018-08-27 | 2018-11-30 | 长春理工大学 | A kind of seawater pond calibration system based on seawater transmitance |
| CN108918472B (en) * | 2018-08-27 | 2020-09-22 | 长春理工大学 | Laser seawater transmittance calibration system based on seawater pool |
| CN110656185A (en) * | 2019-10-30 | 2020-01-07 | 浙江省医学科学院 | Primer group for detecting trichina gene through loop-mediated isothermal amplification, application and method |
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