CN1737108A - Method for separating biotic bacteria from human excrement - Google Patents

Abstract

The invention provides a process for separating probiotic bacteria from human body dejection, which consists of collecting human body dejection with a container having oxygen-free environment, diluting the dejection with oxygen-free solution, filtering with 200-300 mesh sieve, obtaining first level probiotic solution, centrifugalizing at a temperature of 30-38 deg. C to obtain second level probiotic solution, charging into food grade adsorbent for thallus adsorption, obtaining probiotic thallus mixture and producing slow release capsules.

Description

A kind of method of from slop, separating probiotic bacterium

Technical field

The present invention relates to a kind of method of separating probiotic bacterium from slop, this method is separated the probiotic bacterium that grows in the human body from movement, send into human body after treatment again, and its surviving rate is high, can effectively improve the quantity of the intravital beneficial flora of people.

Background technology

Modern biomedical proves, higher organism and its intravital microorganism, the decomposition of material, synthetic with exchange, in storage, conversion and the exchange of energy, the duplicating, transmit and exchange two cover systems are all arranged of genetic material, i.e. the system of host's system and microorganism, the two is independently acted separately, but connect each other, carrying out continual exchange and mutual using.Intravital symbiosis flora of people such as bifidus bacillus, lactobacillus, faecalis, intestinal bacteria, genus bacillus etc., healthy significant to the people.Flora in normal human's enteron aisle is mainly anerobe, and minority is an aerophil, and the former is about 100 times of the latter.Having human body is genera bacillus, Bacterium lacticum, intestinal bacteria and faecalis etc. in the normal microflora of enteron aisle, the minority bacterium that passes by on one's way is still arranged, as streptococcus aureus, Pseudomonas aeruginosa, Bacterium paracolii, gas bacillus, Bacillus proteus, Clostridium perfringens, Candida albicans etc.Under normal circumstances, these microorganisms depend on each other for existence, and condition each other, and keep balance, keep certain quantity and ratio.Under the situation that resistance of human body reduces, normal intestinal flora is suppressed and the quantity minimizing, be called alteration of intestinal flora disease.If probiotic bacterium is lost ground, human body will be suffered from diarrhoea, constipation even more serious disease.

In order to address the above problem; Chinese patent 97125843 discloses a kind of probiotic bacterium and has improved specific immunity of organism specific and the developing application of epidemic disease (bacterium) seedling; this patent relates to probiotic bacterium and is improving specific immunity of organism specific and the developing application of epidemic disease (bacterium) seedling; to body through epidemic disease (bacterium) seedling subcutaneous injection and oral route initial immunity or natural infection acquisition initial immunity; behind certain hour; adopt probiotic bacterium such as bifidus bacillus; the intestinal bacteria of Lactobacterium acidophilum and expression protective antigen gene; respectively with its various combination; carry out immunomodulatory by identical approach, to improve body specific immunity level and protection with initial immunity.Another part Chinese patent 00111083.7 discloses conversion and the modification of a kind of beneficial bacteria of intestinal tract fermenting culture to natural drug, this patent utilization bionics principle, and by the enteron aisle of anthropomorphic dummy's intestinal microecology environment benefit bacterium symbiotic fermentation culture medium the natural drug of any plant or animal-origin is carried out biology and transform and modify, with the biologic activity of improving said natural drug and reduce or eliminate its toxic method.

Aforesaid method only illustrates the beneficial effect of probiotic bacterium, and adopt the mode of traditional acquisition probiotic bacterium to utilize probiotic bacterium, because probiotic bacterium is very strict to environment requirement, and the variation for growing environment is also very responsive, therefore, and after human body enters human body with the probiotic bacterium of cultivating the external environment, its surviving rate is extremely low, often do not reach expected effect, therefore, need to propose a kind of new probiotic bacterium obtain manner.

Summary of the invention

The object of the present invention is to provide a kind of method of from slop, separating probiotic bacterium, this method is separated the probiotic bacterium that grows in the human body from movement, isolated probiotic bacterium has human body environment password, send into human body after treatment again if will have human body environment password probiotic bacterium, its surviving rate is inevitable higher, can effectively improve the quantity of the intravital beneficial flora of people.

The objective of the invention is to be realized by following technical proposals: a kind of method of separating probiotic bacterium from slop is characterized in that:

Use the container of an oxygen-free environment to collect slop;

The slop of collecting is used the anaerobic solution dilution, use the 200-300 mesh sieve to filter, obtain one-level probiotic bacterium solution;

Described one-level probiotic bacterium solution is put into whizzer carry out centrifugation, separation temperature is 30-38 ℃, and separating rotating speed is 500-2000 rev/min, obtains secondary probiotic bacterium solution;

Carry out thalline absorption with adding food-grade adsorbent in the described secondary probiotic bacterium solution, obtain probiotic bacterium body mixture;

Described probiotic bacterium body mixture is made slow releasing capsule.

Purpose of the present invention also can realize by another technical scheme: a kind of method of separating probiotic bacterium from slop is characterized in that:

Use the container of an oxygen-free environment to collect slop;

The slop of collecting is used the anaerobic solution dilution, use the 200-300 mesh sieve to filter, obtain one-level probiotic bacterium solution;

Described one-level probiotic bacterium solution is put into whizzer carry out centrifugation, separation temperature is 30-38 ℃, and separating rotating speed is 500-2000 rev/min, obtains secondary probiotic bacterium solution;

Described secondary probiotic bacterium solution is put into the reverse-osmosis treated device carry out filtering separation, obtain three grades of probiotic bacterium solution;

Described three grades of probiotic bacterium solution are put into the drying plant drying treatment, obtain the probiotic bacterium body.

Because the probiotic bacterium that the present invention obtains has human body environment password, and the human body environment is had natural dependence, after it entered human body once more, its surviving rate was inevitable high, can effectively increase and improve the quantity of the intravital beneficial flora of people.

Embodiment

Embodiment one: a kind of method of separating probiotic bacterium from slop is characterized in that:

Use the container of an oxygen-free environment to collect slop; Described container is a closed defecation device, and deaerated water or distilled water are housed in it, and described movement is the ight soil of human body.

Slop is used the anaerobic solution dilution, use the 200-300 mesh sieve to filter, obtain one-level probiotic bacterium solution; Described anaerobic solution is deaerated water or anaerobic distilled water, and anaerobic solution and excremental weight ratio are 10: 1.

Described one-level probiotic bacterium solution is put into whizzer carry out centrifugation, separation temperature is 30-38 ℃, and separating rotating speed is 500-2000 rev/min, obtains secondary probiotic bacterium solution; In the present embodiment, should select corresponding rotating speeds,, improve separating effect so that reduce the damage of thalline according to different mushrooms.

Carry out thalline absorption with adding food-grade adsorbent in the described secondary probiotic bacterium solution, obtain probiotic bacterium body mixture; Described sorbent material is a lime carbonate.The weight ratio of probiotic bacterium solution and sorbent material is 20: 1.

Use conventional slow releasing capsule manufacturing technology to make slow releasing capsule in probiotic bacterium body mixture.

This slow releasing capsule can be sent in the human body from digestive tube, also can send in the human body from the human anus, because isolating probiotic bacterium has human body environment password, therefore, the human body that separates probiotic bacterium should be corresponding with the human body that probiotic bacterium is sent into.

In the present embodiment, described probiotic bacterium comprises: bifidumbacterium bifidum, bifidobacterium breve, bifidus longum bb, bifidobacteria infantis, lactobacillus acidophilus.

Embodiment two: a kind of method of separating probiotic bacterium from slop is characterized in that:

Use the container of an oxygen-free environment to collect slop; Described container is a closed defecation device, and deaerated water or distilled water are housed in it, and described movement is the ight soil of human body.

Slop is used the anaerobic solution dilution, use the 200-300 mesh sieve to filter, obtain one-level probiotic bacterium solution; Described anaerobic solution is deaerated water or anaerobic distilled water, and anaerobic solution and excremental weight ratio are 10: 1.

Described one-level probiotic bacterium solution is put into whizzer carry out centrifugation, separation temperature is 30-38 ℃, and separating rotating speed is 500-2000 rev/min, obtains secondary probiotic bacterium solution; Select corresponding rotating speeds according to different mushrooms.

Secondary probiotic bacterium solution is put into conventional reverse-osmosis treated device carry out filtering separation, obtain three grades of probiotic bacterium solution; In the present embodiment, should select corresponding reverse osmosis membrane according to different mushrooms, improve separating effect, the process of reverse-osmosis treated can be that one-level also can be multistage, so that obtain the higher probiotic bacterium of purity.

Three grades of probiotic bacterium solution are put into conventional drying plant drying treatment, obtain the probiotic bacterium body.The temperature range of drying treatment is 10-40 ℃.

In the present embodiment, mainly adopt PCR to add electrophoresis, gene chip or order-checking mode to the analyzing and testing of probiotic bacterium body, the mode that probiotic bacterium is sent into digestion mainly adopts slow releasing capsule or bowel lavage means.

Claims (2)

1, a kind of method of separating probiotic bacterium from slop is characterized in that:

Use the container of an oxygen-free environment to collect slop;

The slop of collecting is used the anaerobic solution dilution, use the 200-300 mesh sieve to filter, obtain one-level probiotic bacterium solution;

Described one-level probiotic bacterium solution is put into whizzer carry out centrifugation, separation temperature is 30-38 ℃, and separating rotating speed is 500-2000 rev/min, obtains secondary probiotic bacterium solution;

Carry out thalline absorption with adding food-grade adsorbent in the described secondary probiotic bacterium solution, obtain probiotic bacterium body mixture;

Described probiotic bacterium body mixture is made slow releasing capsule.

2, a kind of method of separating probiotic bacterium from slop is characterized in that:

Use the container of an oxygen-free environment to collect slop;

The slop of collecting is used the anaerobic solution dilution, use the 200-300 mesh sieve to filter, obtain one-level probiotic bacterium solution;

Described one-level probiotic bacterium solution is put into whizzer carry out centrifugation, separation temperature is 30-38 ℃, and separating rotating speed is 500-2000 rev/min, obtains secondary probiotic bacterium solution;

Described secondary probiotic bacterium solution is put into the reverse-osmosis treated device carry out filtering separation, obtain three grades of probiotic bacterium solution;

Described three grades of probiotic bacterium solution are put into the drying plant drying treatment, obtain the probiotic bacterium body.