CN1737010A - Galactose albumin preparation method - Google Patents
Galactose albumin preparation method Download PDFInfo
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- CN1737010A CN1737010A CN 200410046676 CN200410046676A CN1737010A CN 1737010 A CN1737010 A CN 1737010A CN 200410046676 CN200410046676 CN 200410046676 CN 200410046676 A CN200410046676 A CN 200410046676A CN 1737010 A CN1737010 A CN 1737010A
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- albumin
- semi
- distilled water
- tri
- lactosi
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Abstract
The invention provides a process for preparing cerebrose albumin which comprises, synthesizing galactosyl albumin from lactose and albumin through amine reduction method, making ferroferric oxide magnetic nano granules with titration hydrolyzing method, then mixing cerebrose albumin, ferriferrous oxide magnetic particles and pure doxorubicin hydrochloride by a finite proportion, finally carrying out ultrasonic homogenization, curing by heating, and ethyl ether scouring in purified cotton seed oil.
Description
Technical field
The present invention relates to a kind of preparation method who is used for the treatment of the liver-cancer medicine targeting vector.
Technical background
Liver cancer is one of modal malignant tumour in the world, and chemotherapy is the important means of treatment liver cancer.In recent years, because the raising of early diagnosis level the excision rate of liver cancer is significantly improved more in the past, but postoperative easily recurs.The one of the main reasons of recurrence is that the postoperative liver is residual many small cancer kitchen ranges.Carry out chemotherapy at these minimum cancer kitchen ranges, not only be difficult to obtain satisfied curative effect, and can not eliminate chemotherapeutics Normocellular toxicity.
Summary of the invention
Purpose of the present invention is will provide a kind of to have that target is strong, medicine comprises rate height, the controlled albuminous preparation method of semi-lactosi of drug release rate.
The albuminous preparation method of semi-lactosi of the present invention is: lactose and albumin are synthesized the galactosyl albumin with reduction amine method, adopt the titration hydrolysis method to prepare the ferroferric oxide magnetic nano grain.Semi-lactosi albumin, ferroferric oxide magnetic nano grain and pure hydrochloric acid Zorubicin are mixed by a certain percentage,, can prepare the semi-lactosi albumin by technologies such as ultrasonic emulsification in the purified cotton seed oil, the sex change that is heating and curing, ether washings.
The semi-lactosi albumin that the present invention obtains has the liver property that significantly becomes to liver, and its Targeting Performance significantly reduces the toxicity of chemotherapeutics, can improve curative effect.Can also improve the antitumous effect of chemotherapeutics, significantly suppress the growth of minimal disease, reduce postoperative recurrence greatly.Tumor-bearing rat is obviously prolonged lifetime.
Embodiment
Introduce the albuminous method of a semi-lactosi of preparation below in conjunction with embodiment.
Getting albumin 140mg, lactose 760mg and sodium cyanoborohydride 504mg is put in the beaker, add tri-distilled water 200ml, place 37 ℃ of constant temperature water tanks, after 2 hours, getting PH and be 8 phosphate buffer solution, the eiweissmilch liquid glucose is transferred to PH is 7.4, continues thermostat(t)ed water and educates 120 hours.Albumin gala liquid glucose is put into homemade Vesica sus domestica semi-permeable membranes, put into tri-distilled water dialysis three days, change tri-distilled water therebetween every day.To dialyse back liquid through dextrane gel SephedaxG-25 column chromatography for separation, be purified into product solution, get powder, promptly get the product that the present invention makes through frost drying.
Claims (1)
1, the albuminous preparation method of a kind of semi-lactosi, making and settlement are equipped with a semi-lactosi albumin, the steps include: to get albumin 140mg, lactose 760mg and sodium cyanoborohydride 504mg are put in the beaker, add tri-distilled water 200ml, place 37 ℃ of constant temperature water tanks, after 2 hours, getting PH and be 8 phosphate buffer solution, the eiweissmilch liquid glucose is transferred to PH is 7.4, continuing thermostat(t)ed water educated 120 hours, albumin gala liquid glucose is put into homemade Vesica sus domestica semi-permeable membranes, put into tri-distilled water dialysis three days, change tri-distilled water therebetween every day, the back liquid of will dialysing is purified into product solution through dextrane gel SephedaxG-25 column chromatography for separation, get powder through frost drying, be the product that the present invention makes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410046676 CN1737010A (en) | 2004-08-19 | 2004-08-19 | Galactose albumin preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410046676 CN1737010A (en) | 2004-08-19 | 2004-08-19 | Galactose albumin preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1737010A true CN1737010A (en) | 2006-02-22 |
Family
ID=36079979
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410046676 Pending CN1737010A (en) | 2004-08-19 | 2004-08-19 | Galactose albumin preparation method |
Country Status (1)
| Country | Link |
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| CN (1) | CN1737010A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102949346A (en) * | 2011-08-26 | 2013-03-06 | 南京大学 | Protein medicine-carrying nano particle synthesis method |
| CN107788981A (en) * | 2017-11-06 | 2018-03-13 | 华南师范大学 | A kind of detection means and method of the imaging of spy Early pancreatic carcinoma |
| CN109157662A (en) * | 2018-06-06 | 2019-01-08 | 浙江海正药业股份有限公司 | A kind of human serum albumins-adriamycin cross-linking agent nano particle and its application |
-
2004
- 2004-08-19 CN CN 200410046676 patent/CN1737010A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102949346A (en) * | 2011-08-26 | 2013-03-06 | 南京大学 | Protein medicine-carrying nano particle synthesis method |
| CN102949346B (en) * | 2011-08-26 | 2014-02-26 | 南京大学 | Protein medicine-carrying nano particle synthesis method |
| CN107788981A (en) * | 2017-11-06 | 2018-03-13 | 华南师范大学 | A kind of detection means and method of the imaging of spy Early pancreatic carcinoma |
| CN109157662A (en) * | 2018-06-06 | 2019-01-08 | 浙江海正药业股份有限公司 | A kind of human serum albumins-adriamycin cross-linking agent nano particle and its application |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |