CN1736991A - A kind of nitrogen heterocyclic ring beta-elemene piperazine alkyl its preparation of analog derivative and application - Google Patents
A kind of nitrogen heterocyclic ring beta-elemene piperazine alkyl its preparation of analog derivative and application Download PDFInfo
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Abstract
A kind of nitrogen heterocyclic ring beta-elemene piperazine alkyl analog derivative has as right constitutional features.The present invention has searched out a kind of beta-elemene derivative of highly active novel structure, thereby can formulate the beta-elemene derivative new drug of better efficacy.
Description
Technical field:
The present invention relates to new nitrogen heterocyclic ring beta-elemene derivative and preparation method thereof, relate to intermediate of synthetic described nitrogen heterocyclic ring beta-elemene derivative and preparation method thereof, and relate to the application of described nitrogen heterocyclic ring beta-elemene derivative.
Background technology:
Beta-elemene is the main component of the Elemenum that extracts from the herbal medicine curcuma zedoary.Be country's two class non-cell toxicity antitumor drugs of China's independent development development.At present, be that the elemene emulsion of main component is " Western medicine two kind new medicines " by the health ministry approval with the beta-elemene, and be applicable to that three phases are clinical.But its derivative is carried out systematic research work big progress is not arranged always owing to there is the isomer separation difficulty.(Vig，O.P.Ram?Bhagat；Atwal，K.S.；Bari，S.S.J.Indian?Chem.Soc.，1975，257-260)。Chinese patent CN 1153168; Chinese patent CN 1462745,2003; Chinese patent CN 1462746,2003; Chinese patent CN 1153167,1997; Zhou Hongyu etc., the progress of Elemenum Anticancer Effect and Mechanism, Chinese clinical tumor, 2000,27, the water-insoluble characteristic limitations that studies show that beta-elemene of documents such as 392-394 its clinical application, but some nitrogen-containing heterocycle compounds have higher anti-cancer activity.
Summary of the invention:
The object of the present invention is to provide a kind of nitrogen heterocyclic ring beta-elemene derivative of novel structure, this compounds structure activity relationship is inquired into, seek the beta-elemene derivative of highly active novel structure, thus the beta-elemene derivative new drug of initiative better efficacy.
The invention provides nitrogen heterocyclic ring beta-elemene derivative, particularly nitrogen heterocyclic ring beta-elemene amide derivatives and nitrogen heterocyclic ring beta-elemene piperazine alkyl analog derivative, it has as shown in the formula I or formula II structure:
Formula I
Wherein:
N is the integer of 0-3;
M is the integer of 0-1;
X, X1, X2, X3 are C independently of one another, N, O, or S; X can also be a key;
R represents H, perhaps represents the F in ortho position on aryl of living in or the heteroaryl and/or a position and/or the contraposition, Cl, Br, I, CN, or NO2; Perhaps representative randomly has one or several substituent C1-C20 alkyl, C1-C20 alkoxyl group, C1-C20 alkyl C6-C20 aryl, the C6-C20 aryl, C2-C20 thiazolinyl or C2-C20 alkynyl, C1-C20 ether, the C1-C20 thioether group, the C1-C20 ester group, C1-C20 amide group, C1-C20 carbonyl, the C1-C20 carboxyl, above-mentioned group randomly has and is selected from O, S, or the heteroatoms of N; Perhaps representative has one or several and is selected from O, S, and N, or the C6-C20 heteroaryl of halogen atom randomly contain O in the chain or on its substituting group, S, N, the C6-C20 aryl C1-C20 alkyl of halogen atom; Described substituting group is selected from: H, and the C1-C4 alkyl, halogen atom, or trifluoromethyl,
Wherein the hexanaphthene skeleton has three chiral centres.
Preferably, the invention provides nitrogen heterocyclic ring beta-elemene derivative with formula I or formula II, wherein:
N is the integer of 0-3;
M is the integer of 0-1;
X, X1, X2, X3 are C independently of one another, N, O, or S; X can also be a key;
R represents H, perhaps represents the F of ortho position on aryl of living in or the heteroaryl and/or a position and/or contraposition, Cl, Br, I, CN or NO2; Perhaps representative randomly has one to three substituent C1-C20 alkyl, C1-C20 alkoxyl group, C1-C20 alkyl C6-C20 aryl, the C6-C20 aryl, C2-C20 thiazolinyl or C2-C20 alkynyl, C1-C20 ether, the C1-C20 thioether group, the C1-C20 ester group, C1-C20 amide group, C1-C20 carbonyl, the C1-C20 carboxyl, above-mentioned group randomly has and is selected from O, S, or the heteroatoms of N; Perhaps representative has one to three and is selected from O, S, and N, or the C6-C20 heteroaryl of halogen atom randomly contain O in the chain or on its substituting group, S, N, the C6-C20 aryl C1-C20 alkyl of halogen atom; Described substituting group is selected from: H, C1-C4 alkyl, halogen atom, or trifluoromethyl.
Wherein the hexanaphthene skeleton has three chiral centres.
More preferably, the invention provides nitrogen heterocyclic ring beta-elemene derivative with formula I or formula II, wherein:
N is the integer of 0-2;
M is 1 integer;
X, X1, X2, X3 are C independently of one another, N, O, or S; X can also be a key;
R represents H, perhaps represents the F in ortho position on aryl of living in or the heteroaryl and/or a position and/or the contraposition, Cl, Br, I, CN or NO2; Perhaps representative randomly has one to three substituent C1-C4 alkyl, C1-C4 alkoxyl group, C1-C4 alkyl C6-C20 aryl, the C6-C20 aryl, C2-C4 thiazolinyl or C2-C4 alkynyl, C1-C4 ether, the C1-C4 thioether group, the C1-C46 ester group, C1-C4 amide group, C1-C4 carbonyl, the C1-C4 carboxyl, above-mentioned group randomly has and is selected from O, S, or the heteroatoms of N; Perhaps representative has one to three and is selected from O, S, and N, or the C6-C20 heteroaryl of halogen atom randomly contain O in the chain or on its substituting group, S, N, the C6-C20 aryl C1-C4 alkyl of halogen atom; Described substituting group is selected from: H, C1-C4 alkyl, halogen atom, or trifluoromethyl.
Wherein the hexanaphthene skeleton has three chiral centres.
Most preferably, the invention provides nitrogen heterocyclic ring beta-elemene derivative with formula I or formula II, among its Chinese style I:
N is 1;
M is 1;
Among the formula II:
N is 0;
M is 1;
And in formula I or formula II,
X1, X2, X3 are C independently of one another, N, O, or S; X is a key;
R represents H, perhaps represents the F in ortho position on aryl of living in or the heteroaryl and/or a position and/or the contraposition, Cl, Br, I, CN or NO2; Perhaps representative randomly has one to three substituent C1-C4 alkyl, C1-C4 alkoxyl group, C1-C4 alkyl C6-C20 aryl, the C6-C20 aryl, C2-C4 thiazolinyl or C2-C4 alkynyl, C1-C4 ether, the C1-C4 thioether group, the C1-C46 ester group, C1-C4 amide group, C1-C4 carbonyl, the C1-C4 carboxyl, above-mentioned group randomly has and is selected from O, S, or the heteroatoms of N; Perhaps representative has one to three and is selected from O, S, and N, or the C6-C20 heteroaryl of halogen atom randomly contain O in the chain or on its substituting group, S, N, the C6-C20 aryl C1-C4 alkyl of halogen atom; Described substituting group is selected from: H, C1-C4 alkyl, halogen atom, or trifluoromethyl.
Wherein the hexanaphthene skeleton has three chiral centres.
In most preferred embodiment of the present invention, among the formula I:
N is 1;
M is 1;
Among the formula II:
N is 0;
M is 1;
And in formula I or formula II,
X, X1, X2, X3 are C independently of one another, N, O, or S; X can also be a key;
R represents H, perhaps represents the F in ortho position on aryl of living in or the heteroaryl and/or a position and/or the contraposition, Cl, Br, I, CN, NO2, methoxyl group or trifluoromethyl.
In the above-mentioned definition, the C1-C4 alkyl is a methyl for example, ethyl, and n-propyl, sec.-propyl, normal-butyl, the tertiary butyl, etc.C1-C4 alkyl C6-C20 aryl is an aminomethyl phenyl for example, ethylphenyl, methyl naphthyl etc.The C6-C20 aryl is a phenyl for example, xenyl etc.The C2-C4 thiazolinyl is a vinyl for example, allyl group etc.The C2-C4 alkynyl is an ethynyl for example, proyl etc.The C1-C4 ether is a methoxyl group for example, oxyethyl group, propoxy-, butoxy etc.The C1-C4 thioether group is a methylthio group for example, ethylmercapto group, etc.The C1-C4 ester group is a methanoyl for example, acetoxyl group etc.The C1-C4 amide group is a formamido-for example, acetamido etc.The C1-C4 carbonyl is an ethanoyl for example, propionyl etc.
The present invention also provides and has contained above-mentioned nitrogen heterocyclic beta-elemene derivative, particularly nitrogen heterocyclic ring beta-elemene amide derivatives or nitrogen heterocyclic ring beta-elemene piperazine alkyl analog derivative and pharmaceutical composition that pharmaceutically can received vehicle.
Nitrogen heterocyclic ring beta-elemene derivative of the present invention or composition can be used to prepare various anti-tumor drugs.
Particularly, the invention provides nitrogen heterocyclic ring beta-elemene amide derivatives of the present invention or the nitrogen heterocyclic ring beta-elemene piperazine alkyl analog derivative purposes in the various anti-tumor drugs of preparation.
The present invention provides the preparation method of above-mentioned nitrogen heterocyclic ring beta-elemene derivative in addition, it is characterized in that: by beta-elemene piperazine intermediate, with carboxylic acid halides R '-CO-X ' or halogenated compound R '-X ' reaction, in solvent, make up syntheticly, the structural formula of intermediate is:
Among R '-CO-X ' or R '-X ', X ' is a halogen atom, the alkyl that on behalf of substituted aryl or aryl, R ' replace.
Solvent for use does not have particular requirement for reaction solvent commonly used in the above-mentioned nitrogen heterocyclic ring beta-elemene of the present invention derivative preparation process, as DMC, and DMF, DMSO, toluene or the like.
The midbody compound that is used for synthetic nitrogen heterocyclic ring beta-elemene derivative is provided among the present invention, the beta-elemene piperazine, its synthetic route is as follows:
The solvent of employed solvent for using always in the synthetic described intermediate, as methylene dichloride, toluene, ethanol, trifluoracetic acid etc.
During synthetic described intermediate, the piperazine that has adopted single N protection is as reaction reagent.Single protection to amino can be adopted conventional methodology of organic synthesis, as BOC, and CBz etc.
Heterocycle beta-elemene derivative described in the invention combines beta-elemene and aryl piperazines two class pharmaceutical activity structures, thereby has better water solubility.Particularly can pass through beta-elemene piperazine key intermediate, obtain single highly purified beta-elemene analog derivative, and can carry out synthesizing on a large scale, therefore easily this compounds be carried out the research of the biological activity rule of system with the combination synthesizing mean.Heterocycle beta-elemene derivative of the present invention in addition is by the inhibition active testing to eight kinds of targets such as Collagen Type VI enzyme, type matrix metalloproteinase, type film class matrix metalloproteinase, type film class matrix metalloproteinase, gelatin enzyme A, gelatinase B, protein tyrosine phosphatase esterase and scavenger cell Proteinase, bone marrow serines, in eight kinds of enzyme molecular targets being tested, compare with the beta-elemene parent, its biological activity is improved, especially in the scavenger cell Proteinase, bone marrow serine, this compounds has showed very high biological activity.And the inhibition activity of acylations beta-elemene derivative is apparently higher than alkyl derivative.At this point, might find treatment of arthritis, periodontitis, type ii diabetes and obesity, pulmonary emphysema, blood vessel scleratheroma and novel antitumor drug etc.
Embodiment:
The preparation of example 1 beta-elemene piperazine intermediate
1) beta-elemene 20.4g (0.1mol) places the 500ml chuck three-necked bottle of band thermometer, adds Glacial acetic acid 9g (0.15mol), methylene dichloride 80ml.The cooling reaction solution drips the 148gNaOCl aqueous solution to 0-5 ℃.Behind the reaction 8h, separate organic layer, water layer NaHCO
3Saturated, and extracted with diethyl ether (2 * 70ml), merge organic layer, anhydrous Na
2SO
4Dry.Vacuum is sloughed solvent in water-bath, and obtaining the pale yellow oily liquid body is that the beta-elemene monochloro is two isomer for product, and content is 48.7%.Post separates fast, obtains monochloro two isomer mixtures in generation, can carry out the next step without separating.
2) 0.86g (0.01mol) piperazine is dissolved in the 20ml methylene dichloride places Jacketed bottle, be cooled to-10--5 ℃, argon shield drips 1.20g (BOC)
2The dichloromethane solution 10ml of O.Ambient temperature overnight (0-5 ℃) is warming up to 20 ℃ then and reacts 7h again, TLC monitoring reaction process.After finishing, filter, solid merges organic layer with 2 * 10ml washed with dichloromethane, removes and desolvates, and adds 20ml water, and stirring at room 30min filters, and water layer is saturated with anhydrous K 2CO3, and (3 * 15ml) extractions merge organic layer, use anhydrous Na with ether
2SO
4Dry.Precipitation gets product, white solid, purity 99%, yield 48.8%
3) single BOC protection piperazine that the beta-elemene chlorating mix products 0.24g, the triethylamine 0.11g that obtain and 0.19g are obtained is dissolved in the 20ml dehydrated alcohol.Be warming up to backflow, reaction 24h, TLC monitoring reaction process.Add the 30ml saturated sodium bicarbonate aqueous solution, separate organic layer.Water layer merges organic layer, anhydrous Na with methylene dichloride 3 * 20ml extraction
2SO
4Dry.Obtain yellow oily liquid.Carrying out post separates; get colourless viscous liquid; the single structure piperazine of BOC protection replaces the beta-elemene intermediate in being accredited as the present invention; purity is more than 98%; high resolution mass spectrum test for HRMS (M+H+) 1H-NMR (; 13C-NMR, DEPT-135,1H-COSY, GC-MS and LC-HRMS identify that purity is 96.3%.1H-NMR(CDCl3)：δ，1.004(s，3H)；1.453(s，9H)1.477～1.662(m，6H)；1.705(s，3H)；2.0075(q，J＝2.00，1H)；2.081(m，1H)；2.317(s，4H)；2.912(q，J＝13.26，2H)；3.404(s，4H)4.579(s，1H)；4.810～4.933(m，5H)；5.808(q，J＝10.88，1H)，13C-NMR：δ，16.4，24.7，27.0，28.2，33.1，39.6，39.7，42.0，52.5，52.8，63.3，79.1，109.7，110.9，111.9，147.3，149.9，150.3，154.5；HRMS(M+H+)Calcd.，389.3163；Found，389.3166。
4) with the 3rd) product of step reaction place trifluoracetic acid and methylene dichloride (TFA/DCM=1: 1, in mixing solutions v/v), room temperature vibration 1h, water-bath vacuum removal solvent obtains tawny oily thickness product.Through identifying that required single structure piperazine replaces beta-elemene intermediate, HRMS (M+H
+): Calcd., 289.2638; Found, 289.2642
The universal synthesis method of example 2 nitrogen heterocyclic ring beta-elemene amide derivatives
With the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and triethylamine be mixed with the methylene dichloride mixing solutions of 0.5mol/l, gets this solution 2ml, room temperature vibration half an hour, cooling reaction system to 0～5 ℃.The acyl chlorides 2ml that adds 0.5mol/l.After reaction finishes, add the saturated NaHCO of 3ml
3The aqueous solution, room temperature vibration 30min separates organic layer.Washing organic layer 3 * 3ml.Anhydrous Na
2SO
4Drying, the vacuum precipitation gets product.
The universal synthesis method of embodiment 3 nitrogen heterocyclic ring beta-elemene alkyls derivatives
With the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and triethylamine be mixed with the toluene mixing solutions of 0.5mol/l, gets this solution 2ml, adds aryl chloride or the aryl bromide 2ml of 0.5mol/l.Temperature rising reflux 24 hours.After reaction finishes, add the saturated NaHCO of 3ml
3The aqueous solution, room temperature vibration 30min separates organic layer.Washing organic layer 3 * 3ml.Anhydrous Na
2SO
4Drying, the vacuum precipitation gets product.
Embodiment 4
Synthetic
According to embodiment 2 methods with the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and 2-chloro-3-pyridine formyl chloride synthesize mass spectrometric measurement (M+H
+): Calcd., 428.2385, Found, 428.2262
Embodiment 5
Synthetic
According to embodiment 2 methods the 4th among embodiment 1) step product beta-elemene piperazine intermediate and 3,4, the 5-tri-methyl chloride synthesizes, mass spectrometric measurement (M+H
+): Calcd., 483.3217, Found, 483.2966
Embodiment 6
Synthetic
According to embodiment 3 methods with the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and 2-nitro-4-benzyl chloride chlorine synthesizes mass spectrometric measurement (M+H
+): Calcd., 458.2569, Found, 458.2311
Embodiment 7
Synthetic
According to embodiment 2 methods the 4th among embodiment 1) step product beta-elemene piperazine intermediate and 2,6-diformazan chloro Benzoyl chloride synthesizes mass spectrometric measurement (M+H
+): Calcd., 453.3112, Found, 453.3137
Embodiment 8
Synthetic
According to embodiment 2 methods with the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and 4-itrile group Benzoyl chloride synthesize mass spectrometric measurement (M+H
+): Calcd., 418.2853, Found, 418.2877
Embodiment 9
Synthetic
According to embodiment 3 methods the 4th among embodiment 1) step product beta-elemene piperazine intermediate and 3,4-benzyl dichloride chlorine synthesizes, mass spectrometric measurement (M+H
+): Calcd., 447.2328, Found, 447.2324
Embodiment 10
Synthetic
According to embodiment 2 methods the 4th among embodiment 1) step product beta-elemene piperazine intermediate and 2, the 6-difluoro benzoyl chloride synthesizes, mass spectrometric measurement (M+H
+): Calcd., 429.2712, Found, 4292743
Embodiment 11
Synthetic
According to embodiment 2 methods with the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and 2-trifluoromethyl benzoyl chloride synthesize mass spectrometric measurement (M+H
+): Calcd., 461.2774, Found, 461.2795
Embodiment 12
Synthetic
According to embodiment 2 methods with the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and cyclohexyl formyl chloride synthesize mass spectrometric measurement (M+H
+): Calcd., 399.3370, Found, 399.3368
Embodiment 13
Synthetic
According to embodiment 2 methods with the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and 3-thiophene chloride synthesize mass spectrometric measurement (M+H
+): Calcd., 399.2465, Found, 399.2445
Embodiment 14
Synthetic
According to embodiment 3 methods with the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and 3-nitrobenzyl chlorine synthesizes mass spectrometric measurement (M+H
+): Calcd., 424.2959, Found, 424.2966
Embodiment 15
Synthetic
According to embodiment 2 methods with the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and 6-bromine caproyl chloride synthesize mass spectrometric measurement (M+H
+): Calcd., 465.2475, Found, 465.2490.
Embodiment 16
Synthetic
According to embodiment 2 methods with the 4th among the embodiment 1) step product beta-elemene piperazine intermediate and ring butyryl chloride synthesize mass spectrometric measurement (M+H
+): Calcd., 371.3057, Found, 371.3071
Embodiment 17
Synthetic
According to embodiment 3 methods the 4th among embodiment 1) step product beta-elemene piperazine intermediate and 2,5-two trifluoromethyl benzyl bromines synthesize mass spectrometric measurement (M+H
+): Calcd., 515.2855, Found, 515.2829
Embodiment 18
Active testing to III type film class matrix metalloproteinase
The III type film class matrix metalloproteinase that is used to screen is in the catalytic subunit fragment of expression in escherichia coli and purified III type film class matrix metalloproteinase, has the hydrolytic enzyme activities same with holoenzyme.In the experiment, enzyme concn is 0.12 μ M.The product of benzyl bromine and beta-elemene reaction is 32% to the inhibition activity of III type film class matrix metalloproteinase under 20 μ g/ml concentration, is 4 times of beta-elemene parent.
Embodiment 19
Active testing to people source gelatin enzyme A
Catalyst structure domain purifying and renaturation behind escherichia coli expression of the people source gelatin enzyme A that is used to screen are measured activity with synthetic ultraviolet peptide substrate, carry out inhibitor screening.Among benzyl bromine and the embodiment 1 the 4th) product inhibition activity to people source gelatin enzyme A under 20 μ g/ml concentration of step product beta-elemene piperazine intermediate reaction is 33%, is 3 times of beta-elemene parent.
Embodiment 20
Active testing to II type film class matrix metalloproteinase
What be used to screen is in the catalytic subunit fragment of expression in escherichia coli and purified II type film class matrix metalloproteinase, has the hydrolytic enzyme activities same with holoenzyme.Among 4-bromobenzyl bromine and the embodiment 1 the 4th) product of step product beta-elemene piperazine intermediate reaction is 66.2% to the inhibition activity of II type film class matrix metalloproteinase under 50 μ M concentration, is 2.5 times of beta-elemene parent.
Embodiment 21
Active testing to the scavenger cell Proteinase, bone marrow serine
The scavenger cell Proteinase, bone marrow serine that is used to screen is the catalysis region at expression in escherichia coli and purified scavenger cell Proteinase, bone marrow serine.In the experiment, with known scavenger cell elastatinal Galardin as positive control.2, among 6-dichlorobenzoyl chloride and the embodiment 1 the 4th) product inhibition activity to the scavenger cell Proteinase, bone marrow serine under 50 μ M concentration of step product beta-elemene piperazine intermediate reaction is 91.6%, and beta-elemene parent inhibition activity to the scavenger cell Proteinase, bone marrow serine under 500 μ M concentration is 20.6.
Embodiment 22
Active testing to the scavenger cell Proteinase, bone marrow serine
The scavenger cell Proteinase, bone marrow serine that is used to screen is the catalysis region at expression in escherichia coli and purified scavenger cell Proteinase, bone marrow serine.In the experiment, with known scavenger cell elastatinal Galardin as positive control.2, among 6-benzyl dichloride chlorine and the embodiment 1 the 4th) product inhibition activity to the scavenger cell Proteinase, bone marrow serine under 50 μ M concentration of step product beta-elemene piperazine intermediate reaction is 15.6%, and beta-elemene parent inhibition activity to the scavenger cell Proteinase, bone marrow serine under 500 μ M concentration is 20.6%.
Claims (7)
1, a kind of nitrogen heterocyclic ring beta-elemene piperazine alkyl analog derivative has following constitutional features:
Wherein:
N is the integer of 0-3;
M is the integer of 0-1;
X1, X2, X3 are C independently of one another, N, O, or S;
R represents H, perhaps represents the F in ortho position on aryl of living in or the heteroaryl and/or a position and/or the contraposition, Cl, Br, I, CN, or NO2; Perhaps representative randomly has one or several substituent C1-C20 alkyl, C1-C20 alkoxyl group, C1-C20 alkyl C6-C20 aryl, the C6-C20 aryl, C2-C20 thiazolinyl or C2-C20 alkynyl, C1-C20 ether, the C1-C20 thioether group, the C1-C20 ester group, C1-C20 amide group, C1-C20 carbonyl, the C1-C20 carboxyl, above-mentioned group randomly has and is selected from O, S, or the heteroatoms of N; Perhaps representative has one or several and is selected from O, S, and N, or the C6-C20 heteroaryl of halogen atom randomly contain O in the chain or on its substituting group, S, N, the C6-C20 aryl C1-C20 alkyl of halogen atom; Described substituting group is selected from: H, C1-C4 alkyl, halogen atom, or trifluoromethyl;
Wherein the hexanaphthene skeleton has three chiral centres.
2, according to the described nitrogen heterocyclic ring beta-elemene of claim 1 piperazine alkyl analog derivative, it is characterized in that:
N is the integer of 0-3;
M is the integer of 0-1;
X1, X2, X3 are C independently of one another, N, O, or S;
R represents H, perhaps represents the F of ortho position on aryl of living in or the heteroaryl and/or a position and/or contraposition, Cl, Br, I, CN or NO2; Perhaps representative randomly has one to three substituent C1-C20 alkyl, C1-C20 alkoxyl group, C1-C20 alkyl C6-C20 aryl, C6-C20 aryl C2-C20 thiazolinyl or C2-C20 alkynyl, the C1-C20 ether, C1-C20 thioether group, C1-C20 ester group, the C1-C20 amide group, C1-C20 carbonyl, C1-C20 carboxyl, above-mentioned group randomly have and are selected from O, S, or the heteroatoms of N; Perhaps representative has one to three and is selected from O, S, and N, or the C6-C20 heteroaryl of halogen atom randomly contain O in the chain or on its substituting group, S, N, the C6-C20 aryl C1-C20 alkyl of halogen atom; Described substituting group is selected from: H, C1-C4 alkyl, halogen atom, or trifluoromethyl;
Wherein the hexanaphthene skeleton has three chiral centres.
3, according to the described nitrogen heterocyclic ring beta-elemene of claim 2 piperazine alkyl analog derivative, it is characterized in that:
N is the integer of 0-2;
M is 1 integer;
X1, X2, X3 are C independently of one another, N, O, or S;
R represents H, perhaps represents the F in ortho position on aryl of living in or the heteroaryl and/or a position and/or the contraposition, Cl, Br, I, CN or NO2; Perhaps representative randomly has one to three substituent C1-C4 alkyl, C1-C4 alkoxyl group, C1-C4 alkyl C6-C20 aryl, the C6-C20 aryl, C2-C4 thiazolinyl or C2-C4 alkynyl, C1-C4 ether, the C1-C4 thioether group, the C1-C46 ester group, C1-C4 amide group, C1-C4 carbonyl, the C1-C4 carboxyl, above-mentioned group randomly has and is selected from O, S, or the heteroatoms of N; Perhaps representative has one to three and is selected from O, S, and N, or the C6-C20 heteroaryl of halogen atom randomly contain O in the chain or on its substituting group, S, N, the C6-C20 aryl C1-C4 alkyl of halogen atom; Described substituting group is selected from: H, C1-C4 alkyl, halogen atom, or trifluoromethyl;
Wherein the hexanaphthene skeleton has three chiral centres.
4, according to the described nitrogen heterocyclic ring beta-elemene of claim 3 piperazine alkyl analog derivative, it is characterized in that:
N is 1;
M is 1;
X1, X2, X3 are C independently of one another, N, O, or S;
R represents H, perhaps represents the F in ortho position on aryl of living in or the heteroaryl and/or a position and/or the contraposition, Cl, Br, I, CN, NO2, methoxyl group or trifluoromethyl.
5, a kind of right that contains requires 1 described nitrogen heterocyclic ring beta-elemene piperazine alkyl analog derivative and pharmaceutical composition that pharmaceutically can received vehicle.
6, the preparation method of the described nitrogen heterocyclic ring beta-elemene of a kind of claim 1 piperazine alkyl analog derivative, it is characterized in that: by beta-elemene piperazine intermediate, with halogenated compound R '-X ' reaction, in solvent, make up syntheticly, the structural formula of intermediate is:
Among R '-X ', X ' is a halogen atom, the alkyl that on behalf of substituted aryl or aryl, R ' replace.
7, the described nitrogen heterocyclic ring beta-elemene of claim 1 piperazine alkyl analog derivative is used to prepare the purposes of anti-tumor drug.
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| CN107216283A (en) * | 2017-07-12 | 2017-09-29 | 钱春发 | A kind of beta-elemene derivatives containing dihydropyridine structure and its production and use |
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| CN1052716C (en) * | 1995-12-26 | 2000-05-24 | 中国科学院大连化学物理研究所 | Elemene hydroxyls derivs. and their use as anticancer drugs |
| CN1181063C (en) * | 2002-05-31 | 2004-12-22 | 中国科学院大连化学物理研究所 | Beta-elemen-5-azacycle ramification and its synthetic method |
| CN1462745A (en) * | 2002-05-31 | 2003-12-24 | 中国科学院大连化学物理研究所 | Miazines ramification of beta-elemene and its synthetic method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107216283A (en) * | 2017-07-12 | 2017-09-29 | 钱春发 | A kind of beta-elemene derivatives containing dihydropyridine structure and its production and use |
| CN107216283B (en) * | 2017-07-12 | 2019-11-26 | 钱春发 | A kind of beta-elemene derivatives and its preparation method and application containing dihydropyridine structure |
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