CN1724571A - New saponin and its derivative, its preparation method and its use in medicine - Google Patents

New saponin and its derivative, its preparation method and its use in medicine Download PDF

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CN1724571A
CN1724571A CN 200510040825 CN200510040825A CN1724571A CN 1724571 A CN1724571 A CN 1724571A CN 200510040825 CN200510040825 CN 200510040825 CN 200510040825 A CN200510040825 A CN 200510040825A CN 1724571 A CN1724571 A CN 1724571A
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ethanol
group
macroporous resin
compound
reagent
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CN1300176C (en
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李丰文
刘丽芳
李运曼
王宇新
朱兴祥
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China Pharmaceutical University
Chia Tai Tianqing Pharmaceutical Group Co Ltd
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China Pharmaceutical University
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Abstract

The invention relates to the field of crude medicament, especially a novel saponin and its derivatives extracted and segregated from traditional Chinese medicinal Clematis with a formula (I), the invention also discloses its preparation process and the use of the novel saponin in preparing medicament for treating immunity inflammation.

Description

A kind of new saponin(e and derivative, its preparation method and medicinal use thereof
Technical field
The present invention relates to natural medicine field, be specifically related to extraction separation obtains from the Chinese medicine Root of Sixpetal Clematis a kind of new saponin(e and derivative thereof, its preparation method, and the application of this new saponin(e in preparation treatment immune inflammation disease drug.
Background technology
Root of Sixpetal Clematis records kind for China's pharmacopeia, its crude drug source is ranunculaceae plant Root of Sixpetal Clematis Clematis chinensis Osbeck, the dry root and rhizome of Clematis hexapetala Clematis hexapetala Pall. or northeast clematis Clematis manshurica Rupr..Excavate autumn, removes silt, dries.Have and dispel rheumatism, remove obstruction in channels to relieve pain.Be used for rheumatic arthralgia, numb limbs and tense tendons, the contraction of muscle arteries and veins, joint stuffiness, the bone diseases such as larynx of choking with sobs are rich in saponin component in this several plant, now from kind of oleanane-type triterpene saponin surplus wherein isolation identification goes out ten, its aglycon is mainly Oleanolic Acid and ivy aglycon, pharmacologically actives such as that these saponin(es report has is antitumor, anti-inflammatory.
Immune inflammations such as rheumatoid arthritis are common clinical and frequently-occurring disease, rheumatoid arthritis belongs to autoimmune disorder, belong to the immune inflammation category, the population of nearly 1-2% suffers from rheumatoid arthritis in the world wide, add up according to the Ministry of Health, China arthritic sum surpasses 100,000,000, wherein has 400 all creation rheumatic arthritis patients at least, and pharmacological agent is topmost methods of treatment.At present treat this sick medicine in the world and mainly contain non-steroidal anti-inflammatory drugs (NASID s, as diclofenac, acetylsalicylic acid, Ibuprofen BP/EP, nimesulide and nabumetone etc.), DMARD s(disease-modifying antirheumaticdrugs, as Rheumatrex, golden salt, antimalarial drug, sulfasalazine, trypterygine, tsiklomitsin and ciclosporin etc.), new biological medicine is (as leflunomide, Kineret) and immunomodulator (Rnbrel, Remicade Humira) wait and to treat, but all there is tangible untoward reaction in these medicines, as damage gastrointestinal tract mucosa and cause peptide ulceration, hepatic injury, fash, bone marrow depression, effect poor selectivity are easy to bring out infection etc.Therefore, for effective constituent clear and definite, quality controllable and safely and efficiently the Chinese medicine effective monomer aspect the medicine of immune inflammations such as development rheumatoid arthritis etc., have tempting market outlook and exploitation is worth.
Summary of the invention
The invention discloses new saponin(e of a class and derivative thereof, its general formula is as follows:
Figure A20051004082500051
R wherein 1And R 2Be H, R 3Be a rhamanopyranosyl (rha); Also can be R 1And R 3Be H, R 2Be a rhamanopyranosyl (rha); Can also be R 2And R 3Be H, R 1Be a rhamanopyranosyl (rha).Be that rha-3 can be that 1-4 position, 1-2 position or 1-3 position are connected with glc-4.
The preferred construction formula:
The molecular formula of Compound I is: G 82H 134O 43, molecular weight is: 1806, and fusing point: 223～225 ℃; [α] 20 D-35.79 ° of (C=0.15, H 2O).
Chemistry is by name: 3-O-[α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 4)-β-D-ribopyranosyl-(1 → 3) α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl]-oleanolic acid-28-O-[α-L-rhamnopyranosyl-(1 → 4)-β-D-glueopyranosyl-(1 → 6)-β-D-glucopyranosyl] ester.
New saponin(e of the present invention and derivative thereof can be made medicine separately or with other Chinese medicine and Chinese medical extract assembly.
New saponin(e of the present invention and derivative thereof can mix with pharmaceutically acceptable carrier, are used to prepare the medicine for treatment compositions.Can be prepared into formulation general on the pharmaceutics, as parenteral dosage forms such as gastrointestinal administration formulations such as capsule, tablet, pill, oral liquid, granule, tincture, transdermal patch, sustained release dosage, injection and injection, external preparations.
New saponin(e of the present invention can prepare in order to the below method:
Get clematis chinensis medicinal materials, its crude drug source is ranunculaceae plant Root of Sixpetal Clematis Clematis chinensis Osbeck, the dry root and rhizome of Clematis hexapetala Clematis hexapetala Pall. or northeast clematis Clematis manshurica Rupr..Get clematis chinensis medicinal materials, with the 20-60% alcohol reflux, the extracting solution concentrating under reduced pressure, high speed centrifugation or filtration are on the filtrate after the macroporous resin adsorption, successively with 20-50% buck, water, 20-40% ethanol liquid, that 50-70% ethanol liquid is eluted to effluent liquid is colourless, collect the 50-70% ethanol eluate, evaporated under reduced pressure gets Radix Clematidis total glycosides, Radix Clematidis total glycosides further separates with reverse phase filler through dextrane gel again, promptly.The preferred ammoniacal liquor of buck wherein.
More preferably the preparation method is: with 20～60% alcohol reflux, extracting solution is evaporated to does not have the alcohol flavor, high speed centrifugation (rotating speed is more than 8000 rev/mins) or filtration, after centrifugate or filtrate are gone up macroporous resin column absorption, successively with 20-30% ammoniacal liquor, water, 20% ethanol liquid, that 70% ethanol liquid is eluted to effluent liquid is colourless, collects 70% ethanol eluate, evaporated under reduced pressure, promptly get total saponins, this total saponins is again through Sephadex LH 20 and ODS post or C 8Post can prepare Compound I after further separating.The wherein preferred HPD100 type of macroporous resin, HPD200 type, HPD300 type or 101 type macroporous resins.D101 type macroporous resin most preferably.
Compound I can add acetylation reagent and form acetylate; perhaps according to concrete needs; add other reagent and form new derivative; can also utilize the effect of bioactive enzyme; change the link position of rha-3 and glc-4; change into 1-3 or 1-2 connection as connecting, prepare the compound of general formula (II) by 1-4.
Find that in research pharmacological action such as that the new saponin(e of this class not only has is antitumor in the prior art, anti-inflammatory also has new curative effect, promptly can be used for the treatment of immune inflammation, the preferred rheumatic arthritis of described disease to saponin(e of the present invention.
Usually, when new saponin(e of the present invention and derivative thereof were used for the treatment of, dosage range was 100-500mg/ days, also can be according to the difference and the disease severity of formulation, and using dosage exceeds this scope.
Description of drawings
The mass spectrum of Fig. 1 Compound I
The long-range coupling figure (HMBC) of Fig. 2 Compound I
The hydrogen of Fig. 3 Compound I-hydrogen correlogram (H-HCOSY)
The carbon of Fig. 4 Compound I-hydrogen correlogram (HSQC)
Fig. 5 Compound I 1H-NMR figure
Fig. 6 Compound I 13C-NMR figure
Below by embodiment the present invention is described further, but the present invention is not limited to the described scope of embodiment.
Embodiment
Embodiment 1
Get clematis chinensis medicinal materials, its crude drug source is ranunculaceae plant Root of Sixpetal Clematis Clematis chinensis Osbeck, 10 kilograms of dry root and rhizomes, measure 60% alcohol reflux 2 times with 12 times, each 2 hours, extracting solution is evaporated to does not have the alcohol flavor, through 20000 rev/mins of high speed centrifugations, after HPD100 type macroporous resin column is adsorbed on the supernatant liquor, use 80L 10% ammonia soln successively, 100L water, 60L 20% ethanol liquid, it is colourless that 60L 70% ethanol liquid is eluted to effluent liquid, collects 70% ethanol eluate, evaporated under reduced pressure, promptly get Radix Clematidis total glycosides, this total saponins is again through Sephadex LH-20 and C 18Post (ODS post) is further with after 0,10%, 20%, 30%, 40%, 50%, 60%, 70% methanol solution wash-out, the separation, and drying can prepare Compound I 1 gram.Structural formula is:
Figure A20051004082500081
New saponin I 13C-NMR and 1The H-NMR data
NO. δ(C，ppm) δ(H，ppm)
1 38.66
2 26.35
3 88.43 3.28.brs d，J＝7.4Hz
4 39.28
5 55.73 0.76
6 18.14
7 32.82
8 39.58
9 47.77
10 36.73
11 23.07
12 122.53 5.39，s
13 144.00
14 41.81
15 27.95
16 23.48
17 46.73
18 41.34
19 45.94
20 30.44
21 33.70
22 32.22
23 27.86 1.29，CH 3
24 16.81 1.14，CH 3
25 15.35 0.78，CH 3
26 17.16 1.06，CH 3
27 25.75 1.23，CH 3
28 176.2
29 32.82 0.89，CH 3
30 23.38 0.87，CH 3
3-o-sugar
Ara 1 104.92 4.84，d，J＝5.8Hz
2 75.14 4.57，m
3 74.53 3.93，m
4 69.99 4.29，m
5 65.34 3.84，m 4.27.m
Rha 2 1 101.15 6.23，d，J＝6.5Hz
2 71.63 4.87，brs
3 81.66 4.68.m
4 72.46 4.41，m
5 68.94 4.23
6 18.30 1.58，d，J＝6.0
Rib 1 104.39 5.85，s
2 72.29 4.54，brs
3 69.55 4.95，，m
4 76.21 4.30，m
5 61.58 4.30，m 4.43，m
Glc 3 1 102.85 4.95，m
2 73.80 4.23，m
3 76.34 4.04，m
4 81.54 4.16，m
5 76.06 3.87，d，J＝7.5Hz
6 60.99 4.04，m 4.21，m
Glc
4 1 104.62 5.09，d，J＝8.1Hz
2 74.35 4.01
3 76.47 3.67
4 77.97 4.41，m
5 77.74 4.11，m
6 61.39 4.30，m 4.43，m
Rha 3 1 102.44 5.42，s
2 71.44 4.74，brs
3 72.25 4.43，brs
4 73.55 4.32，m
5 68.26 4.65，m
6 18.20 1.53，d，J＝6.1
28-o-sugar
Glc
1 1 95.34 6.22，d，J＝8.0
2 73.70 4.21，m
3 78.44 4.19，d，J＝8.1，
4 71.18 3.95，m
5 77.89 4.14，m
6 69.01 4.30，m 4.67，m
Glc 2 1 104.55 4.98，m
2 75.05 3.93，m
3 76.21 4.14，m
4 77.97 4.40，m
5 76.85 3.67，d，J＝9.0，
6 61.39 4.43，m 4.30，m
Rha 1 1 102.44 5.85，s
2 72.14 4.67，m
3 72.46 4.54，d，J＝8.9，
4 73.76 4.33，d，J＝9.3
5 70.59 4.29，m
6 18.89 1.69，d，J＝6.2
Analyze m/z (ESI): 1805[M-H through Agilent LC-MSD-Trap-SL instrument], 1659,1497,1335,1189,1027,865,733,587,455.Further determine that through the AgilentTOF high-resolution mass spectrometer its molecular weight is: 1806.8221, molecular formula is: C 82H 134O 43
Get this compound 10mg, after 20% HCl solution hydrolysis, use chloroform extraction, get chloroform solution, after the solvent evaporated, be settled in the 5ml volumetric flask with acetonitrile, analyze through HPLC, analysis condition be the C18 post (150 * 4.6mm), moving phase acetonitrile: water (90: 10), detect wavelength: 215nm, flow velocity: 1.0ml/min; The result shows, the retention time consistent (6.239min) of the retention time of aglycon chromatographic peak and Oleanolic Acid reference substance chromatographic peak.Show that further the aglycon behind this compound hydrolysis is an Oleanolic Acid; In addition, analyze, show, contain glucose, rhamnosyl, ribose, four kinds of monose compositions of pectinose in the hydrolysate through HPTLC to monose in this idic acid hydrolysis and the basic hydrolysis after product.
Through identifying that this compound is: 3-O-[α-L-pyranose form rhamnosyl-(1 → 4)-β-D-glucopyranose (1 → 4)-β-D glucopyranose (1 → 4)-β-D-pyranose form ribose-(1 → 3) α-L--pyranose form rhamnosyl-(1 → 2)-α-L-pyranose form pectinose]-Oleanolic Acid-28-O-[α-L-pyranose form rhamnosyl-(1 → 4)-β-D-glucopyranose-(1 → 6)-β-D-glucopyranose] ester.
English by name: 3-O-[α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 4)-β-D-ribopyranosyl-(1 → 3) α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl]-oleanolic acid-28-O-[α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl] ester.
Embodiment 2
The new saponin monomer of Root of Sixpetal Clematis is to the influence of rat assist agent arthritis
One, test materials
1.1 medicine and reagent
Be subjected to reagent: the new saponin monomer of embodiment 1 preparation
Medicine Dosage
New saponin monomer 0.0234g medicinal extract=1g medicinal material
The dosage conversion:
Rat dosage: new saponin monomer high and low dose of the present invention is 28.0mg extract powder/kg and 14.0mg extract powder/kg
Dexamethasone sodium phosphate injection: Wuzhong, Jiangsu Suzhou No.6 Pharmaceutical Factory of Industry Co.,Ltd produces.
Lot number: 020802 (1ml: 5mg); People's consumption 4mg/ day/people, 0.07mg/kg; Rat dosage: 0.07mg/kg (people) * 6 times=0.4mg/kg
Acetylsalicylic acid: Nanjing Hencer Pharmaceutical Factory
Freund ' s Freund's complete adjuvant: provide by sigma company.
Mda (MDA) is measured test kit: Nanjing is built up bio-engineering research and is provided, lot number: 20041214.
Superoxide-dismutase (SOD) is measured test kit: bio-engineering research institute, lot number: 20041212 are built up in Nanjing.
Two, test method and result
2.1 be subjected to the influence of reagent prevention administration to rat primary adjuvant-induced arthritis:
Get 50 of 140g-190g male rats, be divided into 5 groups at random by body weight, 10 every group, be respectively model control group (0.5%CMC-Na), positive 1 group (dexamethasone 0.4mg) and positive 2 groups (acetylsalicylic acid 500mg), be subjected to each two dosage group (high and low) of reagent.Be that high and low dose is respectively 28.0 and 14.0mg extract powder/kg.The administration volume is 0.5ml, each organize every day gastric infusion once, successive administration 7 days.Survey the right back sufficient volume of rat in the 7th day (before causing inflammation) with the glass volumetric method, after the last administration, caused inflammation for right back sufficient intradermal injection 0.05ml Freund ' the s Freund's complete adjuvant of rat in 30 minutes, measure the right back sufficient sole of the foot volume of rat after 18 hours, and calculate its swelling rate (%) and inhibitory rate of intumesce (%), then rat is put to death, cut open and get thymus gland and spleen, calculate its organ index.With t check between each group data work group.Get right back sufficient sole of the foot joint, thymus gland and spleen, do histopathology and detect.The results are shown in Table 1 and table 2.
Swelling rate=(cause scorching metapedes sole of the foot volume-cause scorching front foot sole of the foot volume)/cause scorching front foot sole of the foot volume * 100%
Inhibitory rate of intumesce=(the average swelling rate of the control group-average swelling rate of administration the group)/average swelling rate of control group * 100%
Table 1 is subjected to the influence of reagent to primary adjuvant arthritis rats pedal swelling
( X±S，n＝10)
Group Dosage (mg/kg) Swelling rate (%) Inhibiting rate (%)
The low dexamethasone acetylsalicylic acid model contrast of the new high-new saponin(e of saponin(e 28.0 14.0 0.4 500.0 9.84±8.49* 13.18±4.21* 10.07±6.00** 11.61±2.17* 22.38±12.12 56.06 41.13 55.02 48.13
Annotate: * P＜0.05, compare with model control group * * P＜0.01
By table 1 as seen, the right back pedal swelling of primary (P＜0.05) that can reduce significantly by reagent high and low dose and acetylsalicylic acid group to cause by Freund ' s Freund's complete adjuvant; But the Dexamethasone group utmost point reduces the right back pedal swelling of primary (P＜0.01) that is caused by Freund ' s Freund's complete adjuvant significantly.
Table 2 is subjected to the influence of reagent to primary adjuvant arthritis rats thymus gland and heavy index of spleen and body weight
( X±S，n＝10)
Group Dosage (mg/kg) Body weight (g) Thymus gland (g/100g body weight) Spleen (g/100g body weight)
The low dexamethasone acetylsalicylic acid model contrast of the new high-new saponin(e of saponin(e 28.0 14.0 0.4 500.0 203.2±11.24** 205.9±17.04** 155±9.20 203.6±12.49** 194.8±0.80** 0.151±0.048** 0.157±0.064** 0.063±0.032 0.174±0.072** 0.192±0.10** 1.163±0.204** 1.257±0.308** 0.401±0.076 0.935±0.136** 1.257±0.308**
Annotate: * * P＜0.01, compare with Dexamethasone group
By table 2 as seen, respectively be subjected to reagent group, acetylsalicylic acid and model control group animal thymus, the heavy index of spleen and body weight, utmost point significant difference (P＜0.01) relatively arranged with Dexamethasone group apparently higher than Dexamethasone group; But compare there was no significant difference with model control group.Show to be subjected to reagent that the thymus gland and the spleen of this model do not had obvious restraining effect, and dexamethasone has obvious restraining effect.
Pathology detection result shows, is subjected to relatively with model control group that reagent is low, the right back sufficient sole of the foot articular morphology of high dose group is better than model control group, wherein is subjected to reagent high dose group form and positive controls (dexamethasone and acetylsalicylic acid) form more approaching.But Dexamethasone group acini lienalis volume-diminished, number also reduces.Thymus gland part is substituted by fatty tissue, and thymic tissue endolymph cell number reduces.
2.2 be subjected to the influence of reagent treatment administration to rat Secondary cases adjuvant-induced arthritis:
Get male 50 rats of body weight 140g-190g, measure the left back sufficient sole of the foot volume of rat with the glass volumetric method, right back afterwards sufficient sole of the foot intradermal injection 0.05ml Freund ' s adjuvant causes inflammation, 19 days left back sufficient sole of the foot volumes of measurement rat behind the Yu Zhiyan, weigh in afterwards, be divided into 15 groups at random by body weight, grouping is with 2.1.This day is given the rat oral gavage administration, and be administered once every day, and continuous 7 days, dosage and administration volume were with 2.1.After the last administration, surveyed the left back sufficient sole of the foot volume of rat with the glass volumetric method in 30 minutes, calculate its swelling rate (%) and inhibitory rate of intumesce (%), and observation forelimb, tail, a situation arises for ear's punctation.With the animal sacrificed by exsanguination, get blood and internal organs (thymus gland, spleen, left ankle joint) afterwards, survey serum MDA and SOD content behind the centrifugal blood, weighing thymus gland, spleen weigh, and calculate organ index.And thymus gland, spleen, left back ankle joint are done histopathology detect.With t check between each group data work group.The results are shown in Table 3-5.
Table 3 be subjected to reagent to the influence of Secondary cases adjuvant arthritis rats pedal swelling (X ± S, n=10)
Group Dosage (mg/kg) Swelling rate (%) Inhibiting rate (%)
The low dexamethasone acetylsalicylic acid model contrast of the new high-new saponin(e of saponin(e 28.0 14.0 0.4mg/kg 500 3.94±3.06** 11.23±5.33** 3.06±2.53** 5.12±4.35** 25.64±27.34 85.57 58.90 186.12 81.28
Annotate: * P＜0.05, compare with model control group * * P＜0.01
By table 3 as seen, be subjected to reagent group (high, dosage), Dexamethasone group and acetylsalicylic acid group can extremely significantly reduce the left back pedal swelling of Secondary cases (P＜0.01) that causes by Freund ' s Freund's complete adjuvant; The left back pedal swelling of Secondary cases (P＜0.05) that can reduce significantly by the reagent low dosage to cause by Freund ' s Freund's complete adjuvant.In addition, the incidence of animal forelimb, tail, ear's punctation is to be subjected to reagent (model control group.The incidence of 2 positive control treated animal forelimbs, tail, ear's punctation be subjected to the reagent group suitable.
Table 4 is subjected to reagent to Secondary cases adjuvant arthritis rats thymus gland and heavy index of spleen and body weight
Influence (X ± S, n=10)
Group Dosage (the mg extract powder/kg) Body weight (g) Thymus gland (g/100g body weight) Spleen (g/100g body weight)
The new high-new saponin(e of saponin(e is low 28.0 14.0 277.500±46.89** 282.800±22.53** 0.165±0.038** 0.158±0.048** 0.756±0.046** 0.661±0.077**
The contrast of dexamethasone acetylsalicylic acid model 0.4 500.0 219.100±25.96 283.200±28.13** 279.500±35.05** 0.065±0.012 0.155±0.015** 0.249±0.066** 0.253±0.047 0.727±0.113** 1.022±0.253**
Annotate: * P＜0.05, compare with Dexamethasone group * * P＜0.01
By table 4 as seen, be subjected to reagent group, acetylsalicylic acid group and model control animal body weight and thymus gland, the heavy index of spleen all apparently higher than Dexamethasone group.With Dexamethasone group utmost point significant difference (P＜0.01) is arranged relatively; Be subjected to relatively there was no significant difference of reagent treated animal body weight and thymus gland, the heavy index of spleen and model control group.Show to be subjected to reagent that the thymus gland and the spleen of this model do not had obvious restraining effect, and dexamethasone has obvious restraining effect.
Pathology detection result shows, is subjected to relatively with model control group that reagent is low, the left back sufficient sole of the foot articular morphology of high dose group animal is better than model control group, wherein is subjected to reagent high dose group form and positive controls (dexamethasone and acetylsalicylic acid) form more approaching.But the acini lienalis volume-diminished of dexamethasone treated animal, number also reduces.Thymus gland part is substituted by fatty tissue, and thymic tissue endolymph cell number reduces.
Table 5 be subjected to reagent to the influence of Secondary cases adjuvant arthritis rats serum MDA and SOD (X ± S, n=10)
Group Dosage (the mg extract powder/kg) MDA(U/L) SOD(U/L)
The low dexamethasone acetylsalicylic acid model contrast of the new high-new saponin(e of saponin(e 28.0 14.0 0.4mg/kg 500.0 0.166±0.035** 0.172±0.059** 0.143±0.052** 0.145±0.061** 0.238±0.034 114.989±2.469** 118.045±3.695** 113.579±4.102** 119.566±1.489** 107.670±3.179
By table 5 as seen, be subjected to reagent group (high and low dose) animal serum MDA significantly to be lower than model control group, utmost point significant difference (P＜0.01) relatively arranged with model control group; Respectively be subjected to reagent group, acetylsalicylic acid and Dexamethasone group animal serum SOD to be significantly higher than model control group, utmost point significant difference (P＜0.01) relatively arranged with model control group
Three, conclusion (of pressure testing):
At rat primary adjuvant-induced arthritis model, be subjected to reagent high and low dose and acetylsalicylic acid group prevention administration can reduce the right back pedal swelling of primary (P＜0.05) that causes by Freund ' s Freund's complete adjuvant significantly.But the Dexamethasone group utmost point reduces the right back pedal swelling of primary (P＜0.01) that is caused by Freund ' s Freund's complete adjuvant significantly.Be subjected to reagent group, acetylsalicylic acid and model control group animal thymus, the heavy index of spleen and body weight apparently higher than Dexamethasone group, utmost point significant difference (P＜0.01) relatively arranged with Dexamethasone group; But compare there was no significant difference with model control group.Pathology detection result shows, is subjected to relatively with model control group that reagent is low, the right back sufficient sole of the foot articular morphology of high dose group animal is better than model control group, wherein is subjected to reagent high dose group form and positive controls (dexamethasone and acetylsalicylic acid) form more approaching.But the acini lienalis volume-diminished of dexamethasone treated animal, number also reduces.Thymus gland part is substituted by fatty tissue, and thymic tissue endolymph cell number reduces.
At rat Secondary cases adjuvant-induced arthritis model, be subjected to reagent group (high, dosage), Dexamethasone group and acetylsalicylic acid group treatment administration can extremely significantly reduce the left back pedal swelling of Secondary cases (P＜0.01) that causes by Freund ' s Freund's complete adjuvant; In addition, the incidence of animal forelimb, tail, ear's punctation is to be subjected to reagent group＜model control group.The incidence of 2 positive control treated animal forelimbs, tail, ear's punctation be subjected to the reagent group suitable.Be subjected to reagent group, acetylsalicylic acid group and model control animal body weight and thymus gland, the heavy index of spleen all apparently higher than Dexamethasone group.With Dexamethasone group utmost point significant difference (P＜0.01) is arranged relatively; Be subjected to relatively there was no significant difference of reagent treated animal body weight and thymus gland, the heavy index of spleen and model control group; Pathology detection result shows, is subjected to relatively with model control group that reagent is low, the left back sufficient sole of the foot articular morphology of high dose group animal is better than model control group, is subjected to reagent high dose group form and positive controls (dexamethasone and acetylsalicylic acid) form more approaching.But the acini lienalis volume-diminished of dexamethasone treated animal, number also reduces.Thymus gland part is substituted by fatty tissue, and thymic tissue endolymph cell number reduces.Be subjected to reagent group (high and low dose) animal serum MDA significantly to be lower than model control group, utmost point significant difference (P＜0.01) relatively arranged with model control group; Be subjected to reagent group, acetylsalicylic acid and Dexamethasone group animal serum SOD to be significantly higher than model control group, utmost point significant difference (P＜0.01) relatively arranged with model control group.
Embodiment 3
The tablet that contains the new saponin monomer of the present invention
Get saponin compound I100mg and starch 50mg that embodiment 1 makes, dextrin 50mg mixes, and makes wetting agent with an amount of 30% ethanol, makes softwood, and ordinary method is granulated, and adds an amount of Magnesium Stearate and mixes, and makes tablet.
Embodiment 4
The capsule that contains the new saponin(e of the present invention
Get new saponin monomer 50mg and starch 70mg, dextrin 10mg, Icing Sugar 10mg mix, and make wetting agent with an amount of 30% ethanol, make softwood, and ordinary method is granulated, in the hard capsule of packing into.
Embodiment 5
The slow releasing capsule that contains the new saponin(e of the present invention
Get new saponin monomer 80mg and Vltra tears K15M120mg ethyl cellulose 45cps40mg, lactose 40mg mixes, and is an amount of with 10% polyvinylpyrrolidone k30 ethanolic soln, makes softwood, ordinary method is granulated, and makes slow releasing capsule in the hard capsule of packing into.

Claims (8)

1, the saponin compound of following general formula:
Figure A2005100408250002C1
R 1, R 2Or R 3One of them is rhamanopyranosyl-rha, and two is H in addition.
2, compound according to claim 1, it is following structure:
3, the preparation method of claim 2 compound may further comprise the steps:
Get clematis chinensis medicinal materials, with the 20-60% alcohol reflux, extracting solution concentrating under reduced pressure, high speed centrifugation or filtration, on the filtrate after the macroporous resin adsorption, use 20-50% buck, water, 20-30% ethanol liquid, 50-70% ethanol liquid successively, it is colourless to be eluted to effluent liquid, collects the 50-70% ethanol eluate, evaporated under reduced pressure, get Radix Clematidis total glycosides, Radix Clematidis total glycosides further separates with reversed-phase column through sephadex column again, promptly.
4, the preparation method of claim 3, wherein: the extraction using alcohol of 20-60% 1-3 time, extract solvent load is the 8-20ml/g medicinal material at every turn, extraction time is 1-3 hour, merges ethanol extract, gets ethanol extraction behind the decompression and solvent recovery; Get ethanol extraction, being evaporated to does not have the alcohol flavor after centrifugal, supernatant liquor is splined on macroporous resin column, respectively with 20-30% ammoniacal liquor, water, 20% ethanol, 70% ethanol elution, get 70% ethanol elution thing, reclaim under reduced pressure, the dry extract that obtains is total saponins, and this total saponins is again through Sephadex LH 20 and ODS post or C 8Promptly, wherein macroporous resin was HPD100 type, HPD200 type, HPD300 type or D101 type macroporous resin after post further separated.
5, the preparation method of claim 4, macroporous resin wherein is a D101 type macroporous resin.
6, a kind of pharmaceutical composition wherein contains the claim 1 for the treatment of significant quantity or 2 compound and pharmaceutically acceptable carrier.
7, claim 1 or 2 the compound purposes in preparation treatment immune inflammation disease drug.
8, the purposes of claim 7, wherein immune inflammation is a rheumatoid arthritis.
CNB2005100408257A 2005-06-29 2005-06-29 New saponin and its derivative, its preparation method and its use in medicine Expired - Fee Related CN1300176C (en)

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CN101270144B (en) * 2008-05-13 2010-12-01 中国药科大学 Saponin for inhibiting liveness of tumor necrosis factor alpha, preparing method and medicine uses
CN102120778A (en) * 2010-12-20 2011-07-13 大兴安岭嘉迪欧营养原料有限公司 Method for enriching and purifying clematis saponins of clematis
CN101531721B (en) * 2009-04-28 2011-07-20 中国药科大学 Industrial preparation method for triterpenoid saponin monomer
CN102924618A (en) * 2012-10-30 2013-02-13 中国人民解放军第四军医大学 Triterpenoid saponin anti-myocardial ischemia compound
CN104072626A (en) * 2013-03-26 2014-10-01 正大天晴药业集团股份有限公司 Preparation method for clematichinenoside
CN106822173A (en) * 2017-03-27 2017-06-13 中国药科大学 Application of the AR6 in the medicine for the treatment of or prophylaxis of inflammatory bowel disease is prepared
CN116284200A (en) * 2023-01-12 2023-06-23 成都普思生物科技股份有限公司 Compound extracted and separated from radix clematidis as well as preparation method and application thereof

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JPS57145816A (en) * 1981-03-06 1982-09-09 Wakunaga Yakuhin Kk Assistant for absorption of drug
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CN1193775C (en) * 2000-12-30 2005-03-23 李毅 Rheumatoid disease treating capsule
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101270144B (en) * 2008-05-13 2010-12-01 中国药科大学 Saponin for inhibiting liveness of tumor necrosis factor alpha, preparing method and medicine uses
CN101531721B (en) * 2009-04-28 2011-07-20 中国药科大学 Industrial preparation method for triterpenoid saponin monomer
CN102120778A (en) * 2010-12-20 2011-07-13 大兴安岭嘉迪欧营养原料有限公司 Method for enriching and purifying clematis saponins of clematis
CN102120778B (en) * 2010-12-20 2014-04-02 大兴安岭嘉迪欧营养原料有限公司 Method for enriching and purifying clematis saponins of clematis
CN102924618A (en) * 2012-10-30 2013-02-13 中国人民解放军第四军医大学 Triterpenoid saponin anti-myocardial ischemia compound
CN102924618B (en) * 2012-10-30 2014-10-08 中国人民解放军第四军医大学 Triterpenoid saponin anti-myocardial ischemia compound
CN104072626A (en) * 2013-03-26 2014-10-01 正大天晴药业集团股份有限公司 Preparation method for clematichinenoside
CN106822173A (en) * 2017-03-27 2017-06-13 中国药科大学 Application of the AR6 in the medicine for the treatment of or prophylaxis of inflammatory bowel disease is prepared
CN106822173B (en) * 2017-03-27 2020-01-31 中国药科大学 Application of clematis chinensis glycoside in preparation of medicine for treating or preventing inflammatory bowel diseases
CN116284200A (en) * 2023-01-12 2023-06-23 成都普思生物科技股份有限公司 Compound extracted and separated from radix clematidis as well as preparation method and application thereof

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