CN1721519A - Process for making modified SOD wine - Google Patents

Abstract

The present invention is method of producing modified SOD wine. The technological scheme of the present invention is that the modified SOD wine producing process includes the following steps: dissolving natural SOD inside sodium bicarbonate buffering liquid, dropping lauryl chloride, dropping sodium carbonate to maintain alkali, heating to react before cooling to room temperature, adding pre-cooled acetone, centrifuging to collect precipitate, dissolving the precipitate in double distilled water, centrifuging to obtain supernatant, chromatographic separation in glucosan gel column to collect active enzyme liquid, vacuum freezing to dry, dissolving in deionized water, adding into white spirit and bottling. The unique production process can obtain white spirit with SOD possessing long maintaining period and long half time in body.

Description

The production method of modified SOD wine

One, technical field

The present invention relates to the production method of the production method that a kind of people drink wine, particularly a kind of modified SOD wine.

Two, background technology

Wine is a kind of goods that people often drink.Particularly its spirits culture of China is with a long history.The raising of the material life that becomes increasingly abundant along with development of science and technology and people, people are also more and more higher to the requirement of wine, and its kind is also more and more.A kind of as having at present " pure natural SOD wine ", SOD is the abbreviation of superoxide-dismutase, is a kind of antioxidase, now publicization.If SOD can not normal presence in the human body, then can cause tumour to take place; Show with yeast experiment and can prolong the zymic survival time.SOD also can protect the various metabolic processes of human body, and as the carbohydrate metabolism process, respiratory etc. make the material of these processes not be subjected to the harm of super oxygen molecule; It can remove deleterious oxyradical in the body, radioprotective infringement, anti-harmful chemical; It can also resist the inflammatory reaction of human body, increases the resistivity of cell to infected by microbes, increases people's resistance against diseases and to the provide protection of nerve, prolongs life.Because the good action of SOD uses in more and more products, as makeup, medicine, liquor or the like, the SOD pure natural product on the existing market extract from animal blood or internal organs.The wine that contains the SOD composition is called SOD wine.

But the stable extreme difference of present pure natural SOD in liquid liquor that is to say, this pure natural SOD vigor survival time in liquor is very short, and the phase for sale of liquor is often very long, and wine is not sold off as yet, and its quality goes bad, even can not drink again, moreover pure natural SOD vigor has only about 5-8 minute the people the intravital transformation period, so do not reach should producing effect of drink SOD wine, therefore, pure natural SOD wine is anxious waits to improve and reform.

Three, summary of the invention

At above-mentioned situation, the present invention's purpose just provides a kind of production method of modified SOD wine.Can effectively solve pure natural SOD survival time in liquor problem short and transformation period weak point in human body, guarantee the quality of wine and drunk effect, the technical scheme of its solution is, getting pure natural SOD is dissolved in the sodium bicarbonate buffer liquid, in ice bath, drip lauroyl chloride, agitation and dropping yellow soda ash keeps alkalescence, reduce to the acetone of room temperature adding precooling after the temperature reaction, centrifugal collecting precipitate dissolves in throw out in the bi-distilled water, and the centrifugal insolubles of removing gets supernatant liquor, go up dextrane gel ion chromatography post again and collect enzymic activity liquid step by step, after the vacuum lyophilization, be dissolved in the deionized water, its liquid poured in the liquor stirred evenly again, bottling, its production method uniqueness of the present invention, good product quality is drunk effective, effectively overcome pure natural SOD wine staging life weak point, the SOD vigor is short problem of transformation period in vivo, is that in the system wine industry creates greatly, has opened up wine and has already produced new bright prospect.

Four, embodiment

Below in conjunction with particular case, performance of the present invention is elaborated.

Provide by the aforementioned techniques scheme, the present invention in the specific implementation, its production method (or claim preparation technology) is undertaken by following concrete steps:

Get SOD 6 grams, be dissolved in 30 milliliters and (or be written as 30ml, as follows) the sodium bicarbonate buffer liquid of PH8.0 in, splash into 10 milliliters of lauroyl chlorides in ice bath, 1 mole of yellow soda ash of agitation and dropping makes pH value keep alkalescence, be warming up to 40 ℃, reacted 1 hour, and reduced to 18-20 ℃ rapidly, add 80 milliliters in acetone being chilled to-20 ℃ in advance, centrifugal collecting precipitate, the throw out of collecting (about 5 grams) is dissolved in 15 milliliters the bi-distilled water, and the centrifugal insolubles of removing is collected the supernatant liquor (about about 12m1) after centrifugal, go up dextrane gel ion chromatography post (5 * 75 centimeters) again, fraction collection enzymic activity liquid after its liquid vacuum lyophilization, gets lauric acid modification SOD (about about 4 grams), be dissolved in 20 milliliters the deionized water, its solution is poured in the liquor of 140-700kg, the pneumatic bottling that stirs is modified SOD wine again.Stir and generally can in the wine jar, carry out.How many modification SODs adds, and visual different needs takes the circumstances into consideration to add, and modification SOD is soluble in water, be insoluble to ethanol, therefore, what add, relevant with the purity of liquor, lacked the effect that does not reach modified SOD wine because add, added, can precipitate, adding 4-20 ten thousand unit of activity in testing with every 500ml liquor gets final product, that is to say that aforesaid method can once make 5,600 ten thousand unit of activity/4 grams, join in the liquor of 140-700kg, be one bottle with 500ml and can be made into 280-1400 bottle modified SOD wine.

From the above, the present invention is to the modification of the reactive site (arginine, Histidine, half deamination acid) of pure natural SOD with to the modification at the nonactive position of pure natural SOD (Methionin) with the lauric acid modification technique, the former belongs to the interior finishing of enzyme molecule, the latter belongs to the finishing of enzyme molecule, SOD after the modification has changed former attribute, had stronger vigor and effect, add in the liquor, make wine high-quality more equally, effect is better, prove with experimentation on animals that after measured modified SOD wine is compared with former pure natural SOD wine, has bigger superiority:

1, improves the stability of SOD vigor in liquid liquor greatly, prolonged the quality guaranteed period.Through Henan Province's green cross bio-engineering research the modification SOD vigor in " hundred spring spring SOD wine " has been carried out heat stability test.They are incubated SOD wine sample down at 50 ℃, 60 ℃ respectively, timing sampling is measured the content of SOD unit of activity, (Fractional life (AmlrjandAK) pharmsci 66:785,1977) recommends out under 40 ℃ by test for data with mark validity period method, and it is more than 1 year that the SOD vigor in " SOD wine " is preserved validity period (more than 90%); Former SOD wine has only some months, even shorter.

2, improved the transformation period of SOD vigor greatly.Open flat 2-301077 method through Henan Province's green cross bio-engineering research institute, Molecular Biology Research Lab of University Of Hebei according to patent documentation----spy: select 8 of male guinea pigs (body weight 250-280g) for use, separate modification SOD test group and pure natural SOD control group, 4 every group.Every modification SOD 0.6mg through jugular vein injection physiological saline solution of test group, every of control group is through the pure natural SOD of jugular vein injection physiological saline solution 0.6mg.The venipuncture syringe needle is fixed on skin layer, and the 0.4ml that respectively took a blood sample every 5 minutes measures the SOD unit of activity in the blood plasma.

The result who measures is: the transformation period of pure natural SOD in blood is 5 minutes, and the transformation period of modification SOD in blood is up to more than 40 minutes.

3, this modification SOD has carried out a detection such as total plate count, intestinal bacteria, pathogenic bacterium through Henan Province's product quality supervision and testing institute, and measurement result all meets national food hygienic standard (Henan Province's quality supervision is read No. 1217).

4, select for use Chinese Academy of Medical Sciences animal 30 of purebred Balb/c mouse, body weight 25-30g, be divided into 5 groups at random, every group 6 (3 male 3 is female) behind the animal fasting 8h, presses the administration of 100u/g body weight, dosage series is 100u, 200u, 500u, 1000u/g body weight, control group perfusion physiological saline, result: observed for two weeks continuously, none the dead and poisoning of test group and control animals.Necrotomy: each treated animal of visual inspection, it is unusual not have hyperemia, ulcer and internal organs.Show that modification SOD is without any side effects.

In a word, modified SOD wine of the present invention has improved effective preservation period and consumption value greatly, and passes through for expert statement.Modified SOD wine is succeeded in developing, and is a big innovation and creation of wine industry, must the horn of plenty people live and healthy bringing glad tidings, and wide development is arranged, sell and drink market.

Claims (1)

1, a kind of production method of modified SOD wine, it is characterized in that it being with SOD 6 grams, be dissolved in 30 milliliters the sodium bicarbonate buffer liquid of PH8.0, in ice bath, splash into 10 milliliters of lauroyl chlorides, 1 mole of yellow soda ash of agitation and dropping makes pH value keep alkalescence, is warming up to 40 ℃, reacted 1 hour, reduce to 18-20 ℃ rapidly, add 80 milliliters in acetone being chilled to-20 ℃ in advance, centrifugal collecting precipitate, the throw out of collecting is dissolved in 15 milliliters the bi-distilled water, the centrifugal insolubles of removing is collected the supernatant liquor after centrifugal, goes up dextrane gel ion chromatography post again, fraction collection enzymic activity liquid, after its liquid vacuum lyophilization, the lauric acid modification SOD, be dissolved in 20 milliliters the deionized water, again its solution is poured in the liquor of 140-700kg, stirred.