CN1718575A - Method of extracting taxadol and polysaccharide from taxus fruit and flower - Google Patents
Method of extracting taxadol and polysaccharide from taxus fruit and flower Download PDFInfo
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- CN1718575A CN1718575A CN 200510050861 CN200510050861A CN1718575A CN 1718575 A CN1718575 A CN 1718575A CN 200510050861 CN200510050861 CN 200510050861 CN 200510050861 A CN200510050861 A CN 200510050861A CN 1718575 A CN1718575 A CN 1718575A
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Abstract
A process for extracting taxol and polyose from the fruit and flower of enqlish yew includes such steps as microwave drying, pulverizing immersing in alcohol twice, filtering to obtain liquid and dregs, vacuum concentrating of liquid, reflux concentrating dissolving in deionized water, adding petroether for removing liposoluble impurities, removing petroether, extracting in dichloroethane, vacuum reflux to obtain taxol, loading said dregs in extractor, adding water, boiling, filter, boiling dregs in water, filter, collecting filtrate, concentrating, adding alcohol, depositing, and separating to obtain polyose.
Description
Technical field
What the present invention relates to is a kind of method of extracting taxol and polysaccharide from taxus fruit and spending.
Background technology
Ramulus et folium taxi cuspidatae has another name called Japanese yew, is a kind of world-class rare species.In the period of the 1968-1980, national cancer institute screens discovery from 3500 kinds of natural plant extracts: obtain a kind of material that is taxol from bark and the xylem of Pacific Ocean Ramulus et folium taxi cuspidatae, to suppressing tumour unique curative effect is arranged.To so far, taxol still is considered to best in the world anticarcinogen.In the xylem of Ramulus et folium taxi cuspidatae, also find diabetes, hypertension, arteriosclerosis, chronic nephritis are had the organic compound of better therapeutic action.But wild Ramulus et folium taxi cuspidatae is the first-grade state protection plant, forbids to cut down and adopts.The plantation Ramulus et folium taxi cuspidatae is because of Ramulus et folium taxi cuspidatae breeding, growth extremely slow (after under the seed kind, ability was germinateed in 2 years, and after emerging, the 10cm that only grows tall every year, diameter only increases 0.5cm).We are since 1998, though there is plantation in some provinces, its xylem and skin can be felled utilization, for the time very far away.
We discover, the fruit of Ramulus et folium taxi cuspidatae, also contain taxol in spending and to diabetes, hypertension, the medicative composition of chronic nephritis.
Summary of the invention:
The object of the present invention is to provide a kind of method of extracting taxol and polysaccharide from japanese yew fruit, spending.
The object of the present invention is achieved like this: this method may further comprise the steps:
1, selects taxus fruit or flower for use, it with 75-85 ℃ of microwave dryer, was dried, and made water content less than 8-12% in 30-45 minute;
2, dried taxus fruit or pollen are broken to the 60-70 order;
3, the taxus fruit after will pulverizing is again put into extractor and was added secondary 95% alcohol immersion 1.5-2.0 hour;
4, filter with flame filter press (100 order filter cloth), slag is standby;
5, filtrate decompression is concentrated, return and heat up in a steamer water alcohol, be concentrated into paste;
6, add 2-3 again and doubly measure deionized water, paste extract is dissolved;
7, add the equivalent sherwood oil then, remove oil-soluble impurities;
8, by after separating sherwood oil, add the extraction of equivalent ethylene dichloride;
9, heat up in a steamer ethylene dichloride through reducing pressure back, get paclitaxel extract;
10, slag is put into multi-function extractor, add 8-10 times of water gaging, boil, 100 ℃ are incubated 40-60 minute;
11, filter with flame filter press (100 order filter cloth), filtrate is standby; Filter residue adds 6-8 times of water gaging and boils, and 100 ℃ are incubated 2-3 hour, filter by last method;
12, merge twice filtrate, be concentrated into the 1/8-1/10 of original volume, add 95% ethanol, left standstill 60-80 minute, separate, get the polysaccharide of japanese yew fruit or flower through disk centrifuge to alcohol concn 75-85%;
The present invention mainly adopts the ethanol extraction, extract taxol and polysaccharide from fruit and the flower of Ramulus et folium taxi cuspidatae, the advantage of its extracting method has: adopt this technology, from japanese yew fruit with spending taxol and two kinds of active ingredients whiles of polysaccharide separation and Extraction are come out, equipment is simple, cost is low, taxol, polysaccharide yield height.
Embodiment
Below by embodiment technical scheme of the present invention is further described in detail.
Embodiment 1:
1, selects taxus fruit for use, use microwave dryer under 80 ℃ of temperature it,, make its water content less than 10% through oven dry in 40 minutes;
2, dried taxus fruit is crushed to 70 orders, puts into extractor then;
3, in extractor, added 95% alcohol immersion 2 hours;
4, use flame filter press (100 order filter cloth) to filter then, filter residue is used 95% alcohol immersion 2 hours again, filters, and secondary filtrate merges, and filter residue is standby;
5, filtrate decompression is concentrated, return and heat up in a steamer water alcohol, be concentrated into paste;
6, add 3 times of amount deionized waters, paste extract is dissolved;
7, add the equivalent sherwood oil then, remove oil-soluble impurities, and separate sherwood oil;
8, add the extraction of equivalent ethylene dichloride;
9, reduce pressure back again and heat up in a steamer ethylene dichloride, paclitaxel extract;
10, add filter residue and put into multi-function extractor, add 10 times of water gagings, boil, 100 ℃ are incubated 60 minutes;
11, filter with flame filter press (100 order filter cloth), filtrate is standby; Filter residue adds 8 times of water gagings and boils, and 100 ℃ are incubated 2 hours, filter by last method;
12, merge twice filtrate, be concentrated into 1/10 of original volume, add 95% ethanol, left standstill 80 minutes, separate, get the japanese yew fruit polysaccharide through disk centrifuge to alcohol concn 85%.
Embodiment 2:
1, selects taxus fruit for use, use microwave dryer under 75 ℃ of temperature it,, make water content less than 8% through oven dry in 45 minutes;
2, dry taxus fruit is crushed to 60 orders, puts into extractor then;
3, add 95%7 alcohol immersions and filter with flame filter press (100 order filter cloth) after 1.5 hours, filter residue is used 95% alcohol immersion 2 hours again, filters, and secondary filtrate merges, and filter residue is standby;
4, filtrate decompression is concentrated, return and heat up in a steamer water alcohol, be concentrated into paste;
5, add 2.5 times of amount deionized waters then, paste extract is dissolved;
6, add the equivalent sherwood oil again, remove oil-soluble impurities, and separate sherwood oil;
7, add the extraction of equivalent ethylene dichloride; Heat up in a steamer ethylene dichloride reducing pressure back, paclitaxel extract;
8, filter residue is put into multi-function extractor, add 8.5 times of water gagings, boil, 100 ℃ are incubated 50 minutes;
9, use flame filter press (100 order filter cloth) to filter then, filtrate is standby; Filter residue adds 7 times of water gagings and boils, and 100 ℃ are incubated 2.5 hours, filter by last method;
10, merge twice filtrate, be concentrated into 1/10 of original volume, add 95% ethanol, left standstill 60 minutes, separate, get the japanese yew fruit polysaccharide through disk centrifuge to alcohol concn 85%.
Embodiment 3:
1, selects taxus fruit for use, under 75 ℃ of temperature,, make water content less than 10% through oven dry in 30 minutes with microwave dryer;
2, dry taxus fruit is crushed to 60 orders, puts into extractor then;
3, add 95% alcohol immersion and filter with flame filter press (100 order filter cloth) after 1.5 hours, filter residue is used 95% alcohol immersion 2 hours again, filters, and secondary filtrate merges, and filter residue is standby;
4, filtrate decompression is concentrated, return and heat up in a steamer water alcohol, be concentrated into paste;
5, add 2 times of amount deionized waters, paste extract is dissolved;
6, add the equivalent sherwood oil then, remove oil-soluble impurities, and separate sherwood oil;
7, add the extraction of equivalent ethylene dichloride, reduce pressure back again and heat up in a steamer ethylene dichloride, get paclitaxel extract;
8, filter residue is put into multi-function extractor, add 8 times of water gagings, boil, 100 ℃ are incubated 40 minutes;
9, use flame filter press (100 order filter cloth) to filter then, filtrate is standby; Filter residue adds 6 times of water gagings and boils, and 100 ℃ are incubated 2 hours, filter by last method;
10, merge twice filtrate, be concentrated into 1/10 of original volume, add 95% ethanol, left standstill 60-80 minute, separate, get the japanese yew fruit polysaccharide through disk centrifuge to alcohol concn 85%.
It is identical with the technology of polysaccharide and above-mentioned three embodiment to extract taxol from Ramulus et folium taxi cuspidatae.
Claims (3)
1, a kind of method of extracting taxol and polysaccharide from taxus fruit and spending, this method may further comprise the steps: 1, select taxus fruit or flower for use, with 75-85 ℃ of microwave dryer, oven dry in 30-45 minute makes water content less than 8-12% with it; 2, dried taxus fruit or pollen are broken to the 60-70 order; 3, the taxus fruit after will pulverizing is again put into extractor and was added secondary 95% alcohol immersion 1.5-2.0 hour; 4, filter with flame filter press (100 order filter cloth), slag is standby; 5, filtrate decompression is concentrated, return and heat up in a steamer water alcohol, be concentrated into paste; 6, add 2-3 again and doubly measure deionized water, paste extract is dissolved; 7, add the equivalent sherwood oil then, remove oil-soluble impurities; 8, by after separating sherwood oil, add the extraction of equivalent ethylene dichloride; 9, heat up in a steamer ethylene dichloride through reducing pressure back, get paclitaxel extract; 10, slag is put into multi-function extractor, add 8-10 times of water gaging, boil, 100 ℃ are incubated 40-60 minute; 11, filter with flame filter press (100 order filter cloth), filtrate is standby; Filter residue adds 6-8 times of water gaging and boils, and 100 ℃ are incubated 2-3 hour, filter by last method; 12, merge twice filtrate, be concentrated into the 1/8-1/10 of original volume, add 95% ethanol, left standstill 60-80 minute, separate, get the polysaccharide of japanese yew fruit or flower through disk centrifuge to alcohol concn 75-85%;
2, the method for extracting taxol and polysaccharide from taxus fruit and spending according to claim 1 is characterized in that selecting for use taxus fruit, with its with microwave dryer under 80 ℃ of temperature, through oven dry in 40 minutes, make its water content less than 10%.
3, the method for extracting taxol and polysaccharide from taxus fruit and spending according to claim 2 is characterized in that dried taxus fruit is crushed to 65 orders, puts into extractor then; In extractor, added 95% alcohol immersion 1.8 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510050861 CN1718575A (en) | 2005-07-26 | 2005-07-26 | Method of extracting taxadol and polysaccharide from taxus fruit and flower |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510050861 CN1718575A (en) | 2005-07-26 | 2005-07-26 | Method of extracting taxadol and polysaccharide from taxus fruit and flower |
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| CN1718575A true CN1718575A (en) | 2006-01-11 |
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| CN 200510050861 Pending CN1718575A (en) | 2005-07-26 | 2005-07-26 | Method of extracting taxadol and polysaccharide from taxus fruit and flower |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102397303A (en) * | 2010-09-10 | 2012-04-04 | 厉美生 | Fresh bud, fresh flower and fresh fruit liquid of taxad |
| CN105950399A (en) * | 2016-06-06 | 2016-09-21 | 江苏红豆杉生物科技股份有限公司 | Making method for taxus chinensis fruit wine |
| CN107365283A (en) * | 2017-08-07 | 2017-11-21 | 江苏红豆杉药业有限公司 | A kind of method that taxol light degradation obtains impurity |
-
2005
- 2005-07-26 CN CN 200510050861 patent/CN1718575A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102397303A (en) * | 2010-09-10 | 2012-04-04 | 厉美生 | Fresh bud, fresh flower and fresh fruit liquid of taxad |
| CN105950399A (en) * | 2016-06-06 | 2016-09-21 | 江苏红豆杉生物科技股份有限公司 | Making method for taxus chinensis fruit wine |
| CN107365283A (en) * | 2017-08-07 | 2017-11-21 | 江苏红豆杉药业有限公司 | A kind of method that taxol light degradation obtains impurity |
| CN107365283B (en) * | 2017-08-07 | 2020-12-08 | 无锡紫杉药业有限公司 | Method for obtaining impurities by photodegradation of paclitaxel |
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