CN1709338A - Insulin sensitive-intensifying agent from plant and its preparing method and use - Google Patents
Insulin sensitive-intensifying agent from plant and its preparing method and use Download PDFInfo
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- CN1709338A CN1709338A CN 200510019016 CN200510019016A CN1709338A CN 1709338 A CN1709338 A CN 1709338A CN 200510019016 CN200510019016 CN 200510019016 CN 200510019016 A CN200510019016 A CN 200510019016A CN 1709338 A CN1709338 A CN 1709338A
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Abstract
The present invention relates to an insulin sensitivity-enhancing agent obtained from plant, its preparation method and application. Said insulin sensitivity-enhancing agent is active component extract of Chinese medicinal and edible dual-purpose plant portulaca, in the active component extract of portulaca the content of portulaca total flavone is 20-60% of its total weight. Said active component extract of Chinese medicinal material portulaca can be used for making functional food with the functions of reducing sugar and regulating blood-lipid. Besides, said invention also provides its two preparation processes and their concrete steps.
Description
Technical field
The present invention relates to prevent and treat the plant extract of diabetes and hyperlipidemia, refer to a kind of euglycemic agent that derives from plant and its production and use particularly.
Background technology
The key problem that type 2 diabetes mellitus takes place is the insulin resistant problem, and the patient often reduces the sensitivity of insulin with insulin secretion relative deficiency, fatty tissue, and the fatty acid that causes dissociating in the metabolism disorder of glycolipid matter, the blood plasma increases.Reduce the fatty acid concentration that dissociates in the serum effectively the glucose uptake of insulin-mediated in the surrounding tissue is increased,, also help to correct abnormal carbohydrate metabolism except that improving the metabolism disorder of blood lipid.
As the brand-new antidiabetic drug euglycemic agent of a class, be called as epoch-making achievement on the anti-diabetic history.Such medicine improves the tolerance and the relative pathophysiological change of insulin by receptor--the peroxisome paraphyte γ (PPAR-γ) that excitement plays a crucial role in metabolism control.Euglycemic agent mainly is the glitazone medicine, and its structure is a thiazolidinedione, can increase the sensitivity of tissue to insulin, blood sugar lowering content.In January, 1971, first glitazone medicine troglitazone comes out in states such as Japan and the United States, regrettably after listing just 3 years, just withdraws from market from the whole world rapidly because of serious liver toxicity even liver dead 71 examples that decline.The lower euglycemic agent of toxicity has then appearred in succession, represent medicine that rosiglitazone, pioglitazone etc. are arranged, be characterized in that blood sugar reducing function is strong, other guide such as hypertension, disorders of lipid metabolism etc. to metabolism syndrome also can produce favorable influence simultaneously, using this class medicine is " the many birds of a stone, lifelong benefit ".But As time goes on, also there is hepatotoxic case to occur frequently, the untoward reaction had palpitation of its existence, headache, pruritus, diarrhoea, edema, dyspnea, weight increase or the like, even bring out acute edema because of fluid retention causes potential patients with heart failure.And great majority have the medicines that strengthen the insulin sensitivity effect in the market, or the glitazone Western medicine, not only its quantity of less types, cost an arm and a leg, and its toxic and side effects is day by day serious.
Summary of the invention
Purpose of the present invention will solve the existing deficiency of glitazone Western medicine exactly, provide a kind of with low cost, have no side effect and have a good hypoglycemic fat-regulating effect derive from euglycemic agent of plant and its production and use.
For achieving the above object, the euglycemic agent that derives from plant that the present invention developed is the active ingredient extract of medicine food dual purpose plant Herba Portulacae, and in this Herba Portulacae active ingredient extract, the Herba Portulacae content of total flavone accounts for 20～60% of its gross weight.
In the above-mentioned Herba Portulacae active ingredient extract, when the Herba Portulacae content of total flavone account for its gross weight 40～60% the time, this euglycemic agent is applicable to the preparation medicine, acceptable any excipient on this euglycemic agent and the pharmaceutics or carrier can be combined, make medicine with hypoglycemic fat-regulating effect.
In the above-mentioned Herba Portulacae active ingredient extract, when the Herba Portulacae content of total flavone account for its gross weight 20～40% the time, this euglycemic agent is applicable to the preparation functional food, this euglycemic agent can be added to and make the functional food with hypoglycemic fat-regulating effect in the food.
The above-mentioned preparation method that derives from the euglycemic agent of plant has two kinds, is respectively applied for to obtain to be applicable to the euglycemic agent for preparing the medicine with hypoglycemic fat-regulating effect and be applicable to that preparation has the euglycemic agent of the functional food of hypoglycemic fat-regulating effect.
One of its method may further comprise the steps:
1) gets exsiccant Herba Portulacae coarse powder, adding naoh concentration is that 2.5～3.5% (g/ml), concentration of alcohol are the alkaline alcohol liquid of 50～70% (ml/ml), the addition of alkaline alcohol liquid is 2～4 times of Herba Portulacae coarse powder weight, heating makes its little boiling, extract 3～5 times, merge extractive liquid,, recovery ethanol wherein gets concentrated solution to there not being the alcohol flavor;
2) polyamide column on this concentrated solution, ethanol elution with 92～97% (ml/ml), to eluent is that green and brown color stops, collect eluent, the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, promptly get the Herba Portulacae active ingredient extract that the Herba Portulacae general flavone content accounts for its gross weight 40～60%, can be used as the insulin sensitivity enhancing agent medicine.
Two of its method may further comprise the steps:
1) gets exsiccant Herba Portulacae coarse powder, adding naoh concentration is that 2.5～3.5% (g/ml), concentration of alcohol are the alkaline alcohol liquid of 50～70% (ml/ml), the addition of alkaline alcohol liquid is 2～4 times of Herba Portulacae coarse powder weight, heating makes its little boiling, extract 3～5 times, merge extractive liquid,, recovery ethanol wherein gets concentrated solution to there not being the alcohol flavor;
2) add the Herba Portulacae coarse powder weight of distilled water to 3～5 times in this concentrated solution, boil postcooling to room temperature, leave standstill filtration, the reject filtering residue, gained adds ethanol and hydrochloric acid in the filtrate for the first time again, and making the concentration of alcohol in the filtrate first time is that 50～70% (ml/ml), pH value are 1.5～2.5;
3) the filtration filtrate first time gets filtrate and precipitate thereof for the second time, and the collecting precipitation thing is standby, reclaims the ethanol in the filtrate second time simultaneously, gets the concentrated solution second time;
4) admix kieselguhr in the second time in the concentrated solution, drying makes it be graininess, the alkaline alcohol liquid that adds 3～5 times of weight, concentration sodium hydroxide is that 2.5～3.5% (g/ml), concentration of ethanol are 50～70% (ml/ml) in the alkaline alcohol liquid, extract altogether 2～3 times, merge extractive liquid,, the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, remerge the collected precipitate of step 3), promptly get the Herba Portulacae active ingredient extract that the Herba Portulacae general flavone content accounts for its gross weight 20～40%, can be used as the functional food of hypoglycemic fat-regulating.
The invention has the advantages that: prepared euglycemic agent derives from the medicine food dual purpose plant Herba Portulacae, experimental study finds that the Herba Portulacae total flavones in its active ingredient extract can effectively reduce fasting glucose, improve hyperinsulinemia, thereby the rising insulin sensitivity index shows that it has the effect that strengthens insulin sensitivity.Simultaneously, the Herba Portulacae total flavones has superior accent fat effect, can not only reduce serum triglycerides, T-CHOL and free fatty, and the energy high density lipoprotein increasing, thereby shows that it has good hypoglycemic fat-regulating effect.Thus, the present invention not only has the pharmacological action of glitazone medicine, and has the advantages that can eat for a long time and not produce toxic and side effects, and simultaneously it is cheap and easy to get, can realize truly " the many birds of a stone, lifelong benefit " to numerous diabeticss.
The relevant effect experiment of euglycemic agent of the present invention is as follows: adopt the Wistar male rat, and body weight 180～220g, first adaptability was fed 3 days before the experiment.10 of random chooses are normal group; Other rat modeling as follows: tail vein injection STZ25mg/kg body weight, after feeding for 2 weeks with normal feedstuff, select the rat of impaired glucose tolerance, 10 of sub-model groups (negative control), Herba Portulacae total flavones group (are promptly taken euglycemic agent group of the present invention at random, abbreviation Herba Portulacae MH group) 10,10 of Max EPA groups (positive control), and change and feed the high heat feedstuff, the high heat feedstuff by normal feedstuff, milk powder, sucrose, refining Adeps Sus domestica, egg by 63: 4: 30: 20: 2 part by weight mixes.Herba Portulacae MH group is irritated stomach by Herba Portulacae total flavones dosage 0.2g/kgd, and the Max EPA group is irritated stomach by pharmaceutical quantities 157.5mg/kgd, and normal group and model group gavage isometric normal saline, and all animals are all irritated stomach once morning every day, gives 11 weeks of clothes continuously.Experiment finishes preceding 2 weeks to make glucose tolerance test.Fasting 12～14h when experiment finishes, pentobarbital sodium (40mg/kg body weight) intraperitoneal anesthesia, through abdominal aortic blood 10ml, separation of serum in the 30min is put-70 ℃ of refrigerators and is preserved, and detects in 1 week.
Above-mentioned experiment comes down to adopt euglycemic agent of the present invention--and the Herba Portulacae extractive of general flavone is intervened the type 2 diabetes mellitus rat, to transfer the positive contrast of fat active drug Max EPA.The result shows: Herba Portulacae MH group has significant difference (P＜0.01) for the result who improves carbohydrate metabolism disturbance with model group and Max EPA group, and is suitable with Max EPA group effect for the result of raising insulin sensitivity.It is unusual that this Herba Portulacae MH group can be improved lipid metabolism, wherein remarkable to reduce serum T G level, compared significant difference (P＜0.01) with model group and Max EPA group.Simultaneously, this Herba Portulacae MH group does not have obvious influence to Apo AI and ApoB ratio.By under tabulate 1 and table 2 as can be known, the Herba Portulacae total flavones can significantly reduce fasting glucose and post-prandial glycemia, improves hyperinsulinemia, improves insulin sensitivity; While is triglyceride reducing, T-CHOL and free fatty effectively, improves high density lipoprotein, thereby demonstrates good hypoglycemic fat-regulating effect.
Table 1 is respectively organized relatively (χ ± s) of rat OGTT, FINS and ISI result
| Group | ??FINS ??(uIU/ml) | ??ISI ??(uIU/ml) | ???????????????????????????????????OGTT(mmol/L) | |||
| ??Omin | ??30min | ??60min | ??120min | |||
| Normal group (n=10) model group (n=10) MH organizes (n=10) vs Duo-xi-kang group (n=10) | ??26.5±9.6 ? ??35.8±13.9△ ? ??29.1±12.5# ? ??31.4±7.9 | ??-2.21±0.93 ? ??3.03±0.67△ ? ??-2.34±0.12 *? ??3.01±0.15 | ??3.28±0.95 ? ??834±1.30△△ ? ??7.64±1.25 ? ??6.78±1.17 | ??5.32±0.51 ? ??16.19±8.25△△ ? ??9.57±1.84 *? ??9.47±1.17 | ??6.01±0.27 ? ??16.24±5.82△△ ? ??12.04±1.64# ? ??11.04±1.88 | ??5.14±0.65 ? ??15.61±5.91△△ ? ??8.88±3.70# ? ??8.6±1.64 ** |
Annotate: compare △ P＜0.05, △ △ P＜0.01 with normal group; Compare #P ≈ 0.05 with model group,
*P＜0.05,
*P＜0.01
Table 2 is respectively organized rat blood serum TG, TC, HDL-C, FFA and Apo AI/ApoB ratio result's comparison
| Group | ??TG(mmol/L) | ??TC(mmol/L) | ??HDL-C(mmol/L) | ??FFA(umol/L) | ??ApoAI/ApoB |
| Normal group (n=10) model group (n=10) MH organizes (n=10) Max EPA group (n=10) | ??1.44±0.812 ??2.66+0.768△△ ??1.70±0.366 **??2.03+0.426 | ??2.01±0.320 ??2.22±0.075△ ??1.76±0.219 *??1.67±0.192 * | ??2.34±0.149 ??1.75±0.459 ??3.91±0.455 ??1.76±0.213 | ??428.6±40.4 ??898.7±4241 △△??6619±307.9 *??688.8±270.8 * | ??1.56±0.72 ??1.52±0.48 ??1.37±0.64 ??2.43±168 |
Annotate: compare △ P＜0.05, △ △ P＜0.01 with normal group; Compare with model group
*P＜0.05,
*P＜0.01
The specific embodiment
Below in conjunction with specific embodiment euglycemic agent of the present invention and preparation method thereof is described in further detail:
Embodiment 1:
A kind of preparation method that derives from the euglycemic agent of plant may further comprise the steps:
1) gets pre-dry Herba Portulacae (Partulace Oleracea L) herb 1Kg, be ground into the Herba Portulacae coarse powder that is of a size of about 3mm, add sodium hydroxide and ethanol, be made into concentration and be respectively the alkaline alcohol liquid 3L of 3% (g/ml) and 60% (ml/ml), heating makes its little boiling, extract 2h, collect extracting solution; Add above-mentioned alkaline alcohol liquid 3L in the last medicinal residues again, repeat aforesaid operations twice, heating extraction 1h and 0.5h successively merge the extracting solution of 3 operations then, and 7L is arranged approximately, reclaim wherein ethanol to there not being the alcohol flavor, the about 3000ml of concentrated solution.
2) polyamide column of 1m * 10cm on this concentrated solution with the ethanol elution of 95% (ml/ml), stops when eluent is green and brown color, collection obtains eluent 2L, the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, concentrates, is dried to powder, promptly gets euglycemic agent of the present invention.
With the rutin is reference substance, adopt chromocor compound and aluminum salt formation complex, and measure in the method that visible region obtains the invariant feature absworption peak: precision takes by weighing and adopts the prepared euglycemic agent powder 0.5g of said method, puts to add methanol 30ml, reflux, extract, 2 times in the return channel, each 2h, put cold filtration, methanol extract liquid is changed in the 100ml measuring bottle, residue repeatedly washs with small amount of methanol, washing liquid is incorporated in the 100ml measuring bottle, adds methanol and is diluted to scale and shakes up; Precision is measured diluent 1.0ml and is placed the 25ml measuring bottle then, adds 80% dissolve with ethanol and standardize solution; The accurate standardize solution liquid 1.0ml that draws in back places the 10ml measuring bottle again, add 5% sodium nitrite 0.3ml, place 6min, add 10% aluminum nitrate 0.3ml again, place 6min, add 4% sodium hydroxide 4ml at last, and add water to graduation mark, shaking up, measure its trap at the 510nm place, is that reference substance can calculate Herba Portulacae general flavone content wherein with the rutin.Testing result shows: the Herba Portulacae content of total flavone accounts for 55.6% of its gross weight in the above-mentioned euglycemic agent, and this product can be used for preparing the insulin sensitivity enhancing agent medicine, can be made into all dosage forms that are suitable for such as powder, piece agent, capsule.
Embodiment 2:
A kind of preparation method that derives from the euglycemic agent of plant may further comprise the steps:
1) gets pre-dry Herba Portulacae (Partulace Oleracea L) herb 1Kg, be ground into the Herba Portulacae coarse powder that is of a size of about 2mm, add sodium hydroxide and ethanol, be made into concentration and be respectively the alkaline alcohol liquid 4L of 2.8% (g/ml) and 65% (ml/ml), heating makes its little boiling, extract 2h, collect extracting solution; Add above-mentioned alkaline alcohol liquid 3L in the last medicinal residues again, repeat aforesaid operations three times, heating extraction 1h, 0.5h, 0.5h successively merge the extracting solution of 4 operations then, and 12L is arranged approximately, reclaim wherein ethanol to there not being the alcohol flavor, the about 3500ml of concentrated solution.
2) polyamide column of 1m * 10cm on this concentrated solution with the ethanol elution of 93% (ml/ml), stops when eluent is green and brown color, collection obtains eluent 2.5L, the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, concentrates, is dried to powder, promptly gets euglycemic agent of the present invention.
Adopt and measure with last identical method, testing result shows: the Herba Portulacae content of total flavone accounts for 58.7% of its gross weight in the above-mentioned euglycemic agent, and this product can be used for preparing the insulin sensitivity enhancing agent medicine.
Embodiment 3:
A kind of preparation method that derives from the euglycemic agent of plant may further comprise the steps:
1) gets pre-dry Herba Portulacae (Partulace Oleracea L) herb 1Kg, be ground into the Herba Portulacae coarse powder that is of a size of about 5mm, add sodium hydroxide and ethanol, be made into concentration and be respectively the alkaline alcohol liquid 2L of 3.2% (g/ml) and 58% (ml/ml), heating makes its little boiling, extract 2h, collect extracting solution; Add above-mentioned alkaline alcohol liquid 2L in the last medicinal residues again, repeat aforesaid operations twice, heating extraction 1h and 0.5h successively merge the extracting solution of 3 operations then, and 5L is arranged approximately, reclaim wherein ethanol to there not being the alcohol flavor, the about 2500ml of concentrated solution.
2) polyamide column of 1m * 10cm on this concentrated solution with the ethanol elution of 97% (ml/ml), stops when eluent is green and brown color, l.5L collection obtains eluent, the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, concentrates, is dried to powder, promptly gets euglycemic agent of the present invention.
Adopt and measure with last identical method, testing result shows: the Herba Portulacae content of total flavone accounts for 42.2% of its gross weight in the above-mentioned euglycemic agent, and this product can be used for preparing the insulin sensitivity enhancing agent medicine.
Embodiment 4:
A kind of preparation method that derives from the euglycemic agent of plant may further comprise the steps:
1) gets pre-dry Herba Portulacae (Partulace Oleracea L) herb 1Kg, be ground into the Herba Portulacae coarse powder that is of a size of about 4mm, add sodium hydroxide and ethanol, be made into concentration and be respectively the alkaline alcohol liquid 4L of 2.8% (g/ml) and 63% (ml/ml), heating makes its little boiling, extract 2h, collect extracting solution; Add above-mentioned alkaline alcohol liquid 3L in the last medicinal residues again, repeat aforesaid operations twice, heating extraction 1h and 0.5h successively merge the extracting solution of 3 operations then, and 8L is arranged approximately, reclaim wherein ethanol to there not being the alcohol flavor, the about 3100ml of concentrated solution.
2) polyamide column of 1m * 10cm on this concentrated solution with the ethanol elution of 94% (ml/ml), stops when eluent is green and brown color, collection obtains eluent 2.2L, the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, concentrates, is dried to powder, promptly gets euglycemic agent of the present invention.
Adopt and measure with last identical method, testing result shows: the Herba Portulacae content of total flavone accounts for 48.2% of its gross weight in the above-mentioned euglycemic agent, and this product can be used for preparing the insulin sensitivity enhancing agent medicine.
Embodiment 5:
A kind of preparation method that derives from the euglycemic agent of plant may further comprise the steps:
1) gets pre-dry Herba Portulacae (Partulace Oleracea L) herb 1Kg, be ground into the Herba Portulacae coarse powder that is of a size of about 3mm, add sodium hydroxide and ethanol, be made into concentration and be respectively the alkaline alcohol liquid 3L of 3% (g/ml) and 60% (ml/ml), heating makes its little boiling, extract 2h, collect extracting solution; Add above-mentioned alkaline alcohol liquid 3L in the last medicinal residues again, repeat aforesaid operations twice, heating extraction 1h and 0.5h successively merge the extracting solution of 3 operations then, and 7L is arranged approximately, reclaim wherein ethanol to there not being the alcohol flavor, the about 3000ml of concentrated solution.
2) add distilled water in this concentrated solution to 5L, boil postcooling, leave standstill filtration to room temperature, the reject filtering residue, gained adds ethanol and hydrochloric acid in the filtrate for the first time again, and making the concentration of alcohol in the filtrate first time is that 50% (ml/ml), pH value are about 2.
3) filter filtrate for the first time, get filtrate and precipitate thereof for the second time, the collecting precipitation thing is standby, and this precipitate promptly is the extract that contains part Herba Portulacae total flavones.Reclaim the ethanol in the filtrate second time simultaneously, get the concentrated solution 600ml second time of paste.
4) admix kieselguhr in the second time in the concentrated solution, dry under 80 ℃ of conditions, make it be graininess, add 2L alkaline alcohol liquid, naoh concentration is that 3% (g/ml), concentration of alcohol are 60% (ml/ml) in the alkaline alcohol liquid, extract 2 times, merging obtains extracting solution 3.2L, and the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, remerges the collected precipitate of step 3), be dried to powder, promptly get euglycemic agent of the present invention.
Adopt and measure with last identical method, testing result shows: the Herba Portulacae content of total flavone accounts for 32.2% of its gross weight in the above-mentioned euglycemic agent, this product can be used for preparing the functional food with hypoglycemic fat-regulating effect, as directly making electuary, or add in the daily breads such as bread, steamed bread, noodles.
Embodiment 6:
A kind of preparation method that derives from the euglycemic agent of plant may further comprise the steps:
1) gets pre-dry Herba Portulacae (Partulace Oleracea L) herb 1Kg, be ground into the Herba Portulacae coarse powder that is of a size of about 2mm, add sodium hydroxide and ethanol, be made into that concentration is respectively 3.2% (g/ml), concentration of alcohol is the alkaline alcohol liquid 3.5L of 65% (ml/ml), heating makes its little boiling, extract 2h, collect extracting solution; Add above-mentioned alkaline alcohol liquid 3L in the last medicinal residues again, repeat aforesaid operations three times, heating extraction 1h, 0.5h, 0.5h successively merge the extracting solution of 4 operations then, and 11L is arranged approximately, reclaim wherein ethanol to there not being the alcohol flavor, the about 2500ml of concentrated solution.
2) add distilled water in this concentrated solution to 4L, boil postcooling, leave standstill filtration to room temperature, the reject filtering residue, gained adds ethanol and hydrochloric acid in the filtrate for the first time again, and making the concentration of alcohol in the filtrate first time is that 60% (ml/ml), pH value are about 2.5.
3) filter filtrate for the first time, get filtrate and precipitate thereof for the second time, the collecting precipitation thing is standby, and this precipitate promptly is the extract that contains part Herba Portulacae total flavones.Reclaim the ethanol in the filtrate second time simultaneously, get the concentrated solution 630ml second time of paste.
4) admix kieselguhr in the second time in the concentrated solution, dry under 80 ℃ of conditions, make it be graininess, add 2L alkaline alcohol liquid, naoh concentration is that 3% (g/ml), concentration of alcohol are 60% (ml/ml) in the alkaline alcohol liquid, extract 2 times, merging obtains extracting solution 3.2L, and the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, remerges the collected precipitate of step 3), be dried to powder, promptly get euglycemic agent of the present invention.
Adopt and measure with last identical method, testing result shows: the Herba Portulacae content of total flavone accounts for 38.4% of its gross weight in the above-mentioned euglycemic agent, and this product can be used for preparing the functional food with hypoglycemic fat-regulating effect.
Embodiment 7:
A kind of preparation method that derives from the euglycemic agent of plant may further comprise the steps:
1) gets pre-dry Herba Portulacae (Partulace Oleracea L) herb 1Kg, be ground into the Herba Portulacae coarse powder that is of a size of about 4mm, add sodium hydroxide and ethanol, be made into that concentration is respectively 2.5% (g/ml), concentration of alcohol is the alkaline alcohol liquid 4L of 55% (ml/ml), heating makes its little boiling, extract 2h, collect extracting solution; Add above-mentioned alkaline alcohol liquid 3L in the last medicinal residues again, repeat aforesaid operations twice, heating extraction 1h and 0.5h successively merge the extracting solution of 3 operations then, and 9L is arranged approximately, reclaim wherein ethanol to there not being the alcohol flavor, the about 2000ml of concentrated solution.
2) add distilled water in this concentrated solution to 3L, boil postcooling, leave standstill filtration to room temperature, the reject filtering residue, gained adds ethanol and hydrochloric acid in the filtrate for the first time again, and making the concentration of alcohol in the filtrate first time is that 55% (ml/ml), pH value are about 2.
3) filter filtrate for the first time, get filtrate and precipitate thereof for the second time, the collecting precipitation thing is standby, and this precipitate promptly is the extract that contains part Herba Portulacae total flavones.Reclaim the ethanol in the filtrate second time simultaneously, must be the concentrated solution 400ml second time of clear paste shape.
4) admix kieselguhr in the second time in the concentrated solution, dry under 80 ℃ of conditions, make it be graininess, add 2L alkaline alcohol liquid, naoh concentration is that 2.5% (g/ml), concentration of alcohol are 55% (ml/ml) in the alkaline alcohol liquid, extract 2 times, merging obtains extracting solution 2.7L, and the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, remerges the collected precipitate of step 3), be dried to powder, promptly get euglycemic agent of the present invention.
Adopt and measure with last identical method, testing result shows: the Herba Portulacae content of total flavone accounts for 22.6% of its gross weight in the above-mentioned euglycemic agent, and this product can be used for preparing the functional food with hypoglycemic fat-regulating effect.
Embodiment 8:
A kind of preparation method that derives from the euglycemic agent of plant may further comprise the steps:
1) gets pre-dry Herba Portulacae (Partulace Oleracea L) herb 1Kg, be ground into the Herba Portulacae coarse powder that is of a size of about 3mm, add sodium hydroxide and ethanol, be made into that concentration is respectively 3.5% (g/ml), concentration of alcohol is the alkaline alcohol liquid 3L of 70% (ml/ml), heating makes its little boiling, extract 2h, collect extracting solution; Add above-mentioned alkaline alcohol liquid 3L in the last medicinal residues again, repeat aforesaid operations twice, heating extraction 1h and 0.5h successively merge the extracting solution of 3 operations then, and 8L is arranged approximately, reclaim wherein ethanol to there not being the alcohol flavor, the about 2200ml of concentrated solution.
2) add distilled water in this concentrated solution to 3L, boil postcooling, leave standstill filtration to room temperature, the reject filtering residue, gained adds ethanol and hydrochloric acid in the filtrate for the first time again, and making the concentration of alcohol in the filtrate first time is that 55% (ml/ml), pH value are about 2.5.
3) filter filtrate for the first time, get filtrate and precipitate thereof for the second time, the collecting precipitation thing is standby, and this precipitate promptly is the extract that contains part Herba Portulacae total flavones.Reclaim the ethanol in the filtrate second time simultaneously, must be the concentrated solution 350ml second time of paste.
4) admix kieselguhr in the second time in the concentrated solution, dry under 80 ℃ of conditions, make it be graininess, add 1.5L alkaline alcohol liquid, naoh concentration is that 3% (g/ml), concentration of alcohol are 70% (ml/ml) in the alkaline alcohol liquid, extract 2 times, merging obtains extracting solution 2.6L, and the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, remerges the collected precipitate of step 3), be dried to powder, promptly get euglycemic agent of the present invention.
Adopt and measure with last identical method, testing result shows: the Herba Portulacae content of total flavone accounts for 21.5% of its gross weight in the above-mentioned euglycemic agent, and this product can be used for preparing the functional food with hypoglycemic fat-regulating effect.
Claims (10)
1. euglycemic agent that derives from plant, it is characterized in that: it is the active ingredient extract of medicine food dual purpose plant Herba Portulacae, the Herba Portulacae content of total flavone accounts for 20～60% of its gross weight in this Herba Portulacae active ingredient extract.
2. the euglycemic agent that derives from plant according to claim 1 is characterized in that: the Herba Portulacae content of total flavone accounts for 40～60% of its gross weight in the said Herba Portulacae active ingredient extract.
3. the euglycemic agent that derives from plant according to claim 1 is characterized in that: the Herba Portulacae content of total flavone accounts for 20～40% of its gross weight in the said Herba Portulacae active ingredient extract.
4. described preparation method that derives from the euglycemic agent of plant of claim 2 may further comprise the steps:
1) gets exsiccant Herba Portulacae coarse powder, adding naoh concentration is that 2.5～3.5% (g/ml), concentration of alcohol are the alkaline alcohol liquid of 50～70% (ml/ml), the addition of alkaline alcohol liquid is 2～4 times of Herba Portulacae coarse powder weight, heating makes its little boiling, extract 3～5 times, merge extractive liquid,, recovery ethanol wherein gets concentrated solution to there not being the alcohol flavor;
2) polyamide column on this concentrated solution, with the ethanol elution of 92～97% (ml/ml), be that green and brown color stops to eluent, collect eluent, the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, promptly gets the Herba Portulacae active ingredient extract that the Herba Portulacae general flavone content accounts for its gross weight 40～60%.
5. the preparation method that derives from the euglycemic agent of plant according to claim 4, it is characterized in that: in the said step 1), the size range of Herba Portulacae coarse powder is between 2～5mm, concentration sodium hydroxide is that 3% (g/ml), concentration of ethanol are 60% (ml/ml) in the alkaline alcohol liquid, the addition of alkaline alcohol liquid is 3 times of Herba Portulacae coarse powder weight, extract altogether 3 times, the 1st～3 time extraction time was respectively 2 hours, 1 hour and 0.5 hour.
6. described preparation method that derives from the euglycemic agent of plant of claim 3 may further comprise the steps:
1) gets exsiccant Herba Portulacae coarse powder, adding naoh concentration is that 2.5～3.5% (g/ml), concentration of alcohol are the alkaline alcohol liquid of 50～70% (ml/ml), the addition of alkaline alcohol liquid is 2～4 times of Herba Portulacae coarse powder weight, heating makes its little boiling, extract 3～5 times, merge extractive liquid,, recovery ethanol wherein gets concentrated solution to there not being the alcohol flavor;
2) add the Herba Portulacae coarse powder weight of distilled water to 3～5 times in this concentrated solution, boil postcooling to room temperature, leave standstill filtration, the reject filtering residue, gained adds ethanol and hydrochloric acid in the filtrate for the first time again, and making the concentration of alcohol in the filtrate first time is that 50～70% (ml/ml), pH value are 1.5～2.5;
3) the filtration filtrate first time gets filtrate and precipitate thereof for the second time, and the collecting precipitation thing is standby, reclaims the ethanol in the filtrate second time simultaneously, gets the concentrated solution second time;
4) admix kieselguhr in the second time in the concentrated solution, drying makes it be graininess, the alkaline alcohol liquid that adds 3～5 times of weight, concentration sodium hydroxide is that 2.5～3.5% (g/ml), concentration of ethanol are 50～70% (ml/ml) in the alkaline alcohol liquid, extract altogether 2～3 times, merge extractive liquid,, the ethanol that reclaims is not wherein distinguished the flavor of to there being alcohol, remerge the collected precipitate of step 3), promptly get the Herba Portulacae active ingredient extract that the Herba Portulacae general flavone content accounts for its gross weight 20～40%.
7. the preparation method that derives from the euglycemic agent of plant according to claim 6, it is characterized in that: in the said step 1), the size range of Herba Portulacae coarse powder is between 2～5mm, concentration sodium hydroxide is that 3% (g/ml), concentration of ethanol are 60% (ml/ml) in the alkaline alcohol liquid, the addition of alkaline alcohol liquid is 3 times of Herba Portulacae coarse powder weight, extract altogether 3 times, the 1st～3 time extraction time was respectively 2 hours, 1 hour and 0.5 hour.
8. the preparation method that derives from the euglycemic agent of plant according to claim 6, it is characterized in that: said step 2), add the Herba Portulacae coarse powder weight of distilled water to 3 times in the concentrated solution, the concentration of alcohol in the filtrate is that 60% (ml/ml), pH value are 2 for the first time.
9. the preparation method that derives from the euglycemic agent of plant according to claim 6, it is characterized in that: in the said step 4), admix kieselguhr in the second time in the concentrated solution, dry under 80 ℃ of conditions, make it be graininess, add the alkaline alcohol liquid of 3 times of weight, concentration sodium hydroxide is that 3% (g/ml), concentration of ethanol are 60% (ml/ml) in the alkaline alcohol liquid, extracts altogether 3 times.
10. described purposes that derives from the euglycemic agent of plant of claim 1, it is characterized in that: acceptable any excipient on this euglycemic agent and the pharmaceutics or carrier are combined, make medicine, maybe this euglycemic agent is added to and make functional food in the food with hypoglycemic fat-regulating effect with hypoglycemic fat-regulating effect.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999658A (en) * | 2010-12-07 | 2011-04-06 | 龚泉福 | Nutrition particles with golden aster and snail and preparation method thereof |
| JP2019142846A (en) * | 2018-02-19 | 2019-08-29 | 独立行政法人国立高等専門学校機構 | Anti-obesity composition and anti-obesity agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1288750A (en) * | 1999-09-22 | 2001-03-28 | 李旭海 | Hypoglycemic slimming medicine and its preparation |
| CN1511576A (en) * | 2002-12-31 | 2004-07-14 | 中国中医药科技开发交流中心 | Medicinal composition for preventing and curing diabetes and its preparing method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999658A (en) * | 2010-12-07 | 2011-04-06 | 龚泉福 | Nutrition particles with golden aster and snail and preparation method thereof |
| JP2019142846A (en) * | 2018-02-19 | 2019-08-29 | 独立行政法人国立高等専門学校機構 | Anti-obesity composition and anti-obesity agent |
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