CN1706954A - Construction process of pig serum albumin locus targeting vector for producing foreign protein - Google Patents

Abstract

The present invention constitutes three kinds of targeting vector capable of being located and integrated onto pig serum albumin locus and makes it possible to produce human serum albumin with pig serum. First, the 35 kb, including the 14 kb before translation starting site, all the exons and introns, and 3'-end translation 4 kb, of pig albumin genome are cloned and sequence analyzed. Then, two kinds of targeting vector capable of locating and integrating human serum albumin cDNA onto pig serum albumin and one kind of targeting vector capable of locating and integrating human serum albumin cDNA onto pig serum albumin locus are constituted. These targeting vectors are transfected pig fetus fibroblast or other cells, hitted cells are screened for nuclear transplantation of animal cells, and transgenic pig capable of producing human serum albumin in its serum is obtained.

Description

Be used to produce the pig serum albumin gene seat targeting vector construction process of foreign protein

Technical field

The present invention relates to utilize pig to produce particularly human serum albumin (human serumalbumin of foreign protein, the construction process of three types of targeting vectors HAS), the targeting vector that can use this method to make up is like this produced HAS in porcine blood serum, be convenient to purifying.With these targeting vector transfection porcine fetus fibroblastses or other cell, filter out the cell that hits, be used for the nuclear transplantation animal cloning, make acquisition can in serum, produce the transgenic pig of HSA.

Background technology

Utilizing pig blood to produce HSA is a kind of perfect method.At present, for the production of HSA, raw material is all from human body.Because of demand is big, crowd that need the person of offering is big, causes the person's of offering complicated component, even adopt strict screening test also to have false negative unavoidably.To such an extent as to cause the pollution of blood source goods.If produce HSA, itself just can avoid the pollution of many humanized's pathogeny factors, as multiple model hepatitis virus and HIV (human immunodeficiency virus) with animal.In addition, can carry out stringent regulations and scale collecting production, guarantee the security of raw materials for production, use the raw materials for production of animal-origin that production cost is lowered simultaneously animal.The homology of human albumin and pig is 75.7%.If the albumin of animal blood is very considerable with alternative its output of HSA fully, can reach about 50g/L serum, its protein folding is fully the same with human body with modification, and is convenient to purifying.

In 2000, people have obtained from somatic clone pig, afterwards clone technology was also improved, make the clone pig technology be more suitable for being used for actual production (Jang-Won Lee, Shin-Chih Wu, CindyTian, Production of Cloned Pigs by Whole-Cell Intracytoplasmic Microinjection.Biology of Reproduction, 2003,69,995-1001.Polejaeva IA, Chen S-H, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KHS.Cloned pigs produced by nuclear transfer from adult somatic cells.Nature, 2000; 407:86-90.).Super acute immunological rejection when blocking the allosome organ transplantation, the ideal method is the α in the pig gene-1, the gene knockout of 3-galactoside transferase, like this, pig cell just can not be expressed this epitope, can reduce hyperacute rejection to greatest extent.If express other genes that can alleviate complement system simultaneously, the organ of pig just can really become the abundant source of human organ transplant surrogate.2002, there are a few family units to utilize somatic cell gene rejecting technology and nuclear transfer technology to prepare simultaneously and lacked α-1, the transgenic pig of 3-galactoside transferase gene.This result of study will have been removed a major obstacles for human use's xenogenesis organ, can open up the approach that a gets a good chance of (Dai Y for the human xenogenesis organ of transplanting usefulness for producing, Vaught TD, Broone J, et al.Targeting disruption of thealpha-1,3-galactosyl-transfer-ase gene in cloned pigs.Nature Biotech, 2002,20 (3): 251-255.Lai LX, Kolber SD, Park KW, et al.Production of alpha-1,3-GTknockout pigs by nuclear transfer cloning.Science, 2002,295:1089-1092.).Adopt similar approach, HSA is changed over to fetal fibroblast or other cell of pig with the mode of gene targeting, produce the transgenic pig of HSA through the nuclear transplantation preparation, can produce HAS, and not production porcine hemoglobin (pig serumalbumin, PSA), the prior process of purifying HAS that makes is simpler.Need to make up the targeting vector that contains the HAS gene like this.Because also having no talent, the sequence of PSA genomic gene analyzes and report, making up targeting vector needs 5 ' ending regulating sequence of porcine hemoglobin, genome sequence and 3 ' is held untranslated sequence, need from genomic library, clone these sequences, the structure that is used for targeting vector, but people only know the PSA gene cDNA sequence at present, we based on this, the preparation probe, from the pig DNA library, clone pig serum albumin gene group gene order, carry out complete sequence analysis and (seen sequence table: pig serum albumin gene group gene order), design the targeting vector construction process on this basis, and successfully made up targeting vector.

The PSA genomic gene is longer, 16kb is arranged, the purpose that adds us need be cloned 5 ' ending regulating sequence, genome sequence and 3 ' is held untranslated sequence, and the mean length in λ library is 16kb, we have prepared a plurality of probes from cDNA 5 ' end, genome sequence and 3 ' end respectively like this, screen, reach the purpose that acquisition comprises the full length sequence of pig serum albumin gene group gene 5 ' ending regulating sequence, genome sequence and the untranslated sequence of 3 ' end.

Summary of the invention

For the targeting vector of a kind of energy positioning integration to porcine somatic cell is provided, on the basis of the sequence of having cloned pig serum albumin gene seat 35kb, made up three types targeting vector, be used for porcine somatic cell gene targeting and screening.Provide useful tool for utilizing transgenic animal to produce human serum albumin.The present inventor has taked following technical scheme.

The present inventor from the DNA of pig, clones dna probe with PCR method respectively, after sequential analysis, as the probe of library screening.In the genomic library screening, use probe hybridization and PCR to identify the bonded method, segmentation has cloned PSA 5 ' ending regulating sequence, genome sequence and 3 ' is held untranslated sequence, through sequential analysis, obtain detailed sequence and (seen attached sequence table: pig serum albumin gene group gene order), and with reporter gene after the checking of the liver expression of liver cell and transgenic mouse, be used for the structure of gene targeting carrier.The genome of considering HSA is longer, be 16KB, add homologous sequence and the screening-gene of the regulating and controlling sequence of PSA gene as 5 ' end homologous sequence and 3 ' end, targeting vector is bigger, should not operate, we use the cDNA of HSA gene to be inserted between the 1st exon and the 1st intron of PSA translation initiation site, connect BGH polyA signal sequence behind the cDNA, 3 ' end homologous sequence is part the 1st intron to the .3 intron sequences of pig albumin gene group, 3 ' end of carrier connects the pSV40-tk sequence, made up gene targeting carrier pPHA1 (seeing accompanying drawing 7A), contain antibiotics resistance gene and tk gene in the targeting vector, introduce the loxP sequence at the two ends of resistant gene.Second targeting vector, employing is inserted in the cDNA of HSA gene between porcine hemoglobin translation initiation site and the 14th exon, be connected with the polyA signal sequence behind the cDNA of HSA gene, having loxP sequence resistant gene is inserted in the 14th intron sequences, be built into second targeting vector (pPHA2 sees accompanying drawing 7B).The 3rd targeting vector adopted the genomic full length sequence of HSA, be inserted in after terminator codons pig the 1st exon and 14 exons between (seeing Fig. 7 C).

Description of drawings

The synoptic diagram of Fig. 1 pN53-HA plasmid construction.

The enzyme of Fig. 2 pN53 is cut qualification result.

The synoptic diagram of Fig. 3 pN53-HA-PA plasmid construction.

The enzyme of Fig. 4 pN53-HA is cut qualification result.

Fig. 5 recombinant plasmid pN53-HA-PA enzyme is cut evaluation (ApaI, SacI).

The synoptic diagram that Fig. 6 targeting vector pHPA1 makes up.

The synoptic diagram of Fig. 7 targeting vector pHPA1, pHPA2 and pHPA3.

Embodiment

1 reagent, plasmid and the bacterial strain that uses

In implementation process of the present invention, used following reagent: the random primer labelling test kit, the pGEM-7Zf cloning vector, T support agent box, pGEM-T Vector, the pig genomic library, [a-32p] dCTP (from inferior brightness company), E.coli DH10 β, the pGEM-7Zf plasmid, the Ploxp plasmid, p5ZF-BGHpolyA plasmid (preserve this chamber), E.coli DH5 α competent cells and DNA standard Marker (sky is the epoch product), T4 DNA polymerase (magnificent company product), the Pyrobest archaeal dna polymerase, the T4 archaeal dna polymerase, the big fragment of Klenow, T4DNA ligase enzyme (TAKARA company product), multiple restriction endonuclease (Biolab company and Takara company product); Various primers are synthetic by National Center of Blomedical Analysls.Also have some test kits: QIAprep Spin Miniprep Kit (50) (genome company's product), Gel Extraction Mini Kit (50) (Shanghai China Shun bio-engineering corporation), plasmid extraction kit, PCR purification kit (tieing up special clean company product).

The primer of 2 design and use

Because pig serum albumin gene is bigger, once hybridization is difficult to be sieved to full length gene, therefore designs 3 ' end, 5 ' respectively and holds probe to hybridize.Pig promoter sequence and porcine hemoglobin cDNA sequences Design that primer is announced according to GeneBank, synthetic by National Center of Blomedical Analysls.

5 ' end probe: forward primer D123 is positioned at the pig serum albumin gene promoter region, and reverse primer D61 is positioned on porcine hemoglobin first exon.3 ' end probe: forward primer D81 and reverse primer 82 lay respectively at the zero position and the final position of the 13rd exon.

Forward primer D123:5 '-CAG ATC CAG ACG GCA AAC ACA CGC-3 '

Reverse primer D61:5 '-CTC GAC GAA ACA CAC CCC TGG-3 '

Forward primer D81:5 ' TGG TCC TGA ACC GGT TGT GC 3 '

Reverse primer D84:5 ' CTC ATC CTC AGA AAG TGT GC 3 '

Pig albumin gene sequence according to library screening obtains designs following primer and carries out the amplification of gene fragment, and makes up targeting vector.

P1 5’GTG?ACA?TGG?CTG?ACA?GTT?TGA?TTG?3’

P2 5’AAA?GGT?TAC?CCA?CTT?CAT?TGT?GCC?AAA?GG?3’

P3 5’GTA?AGG?GTA?CCT?GCT?CGA?GAG?TTC?GAT?TTT?TCT?ATT?GTT?C?3’

P4 5’GGG?AAG?CTT?GGT?TCC?CAA?AGA?GGA?CAT?GTT?GGG?GTG?3’

P5 5’ACAVACA?CAG?TGA?CAA?GCA?TCT?CAG?GTA?AGG?TAC?CTG?CTC

GAG?CTA?TAC?TTT?ATA?TTT?TTT?TTT?TCT?TAA?AAG?TAG?3’

P6 5’GGG?AAG?CTT?GGG?CCT?CTG?CTG?CAT?ATG?ATG?GTT?CC?3’

P7 5’GAA?GTG?GGT?AAC?CTT?TAT?TTC?CCT?TCT?TTT?TCT?CTT?TAG?CTC

GGC?TTA?TTC?CAG?GGG?TGT?GTT?TCG?TCG?AG?3’

P8 5’GGG?GTC?GAC?TTA?TAA?GCC?TAA?GGC?AGC?TTG?ACT?TGC?AGC

AAC?AAG?TTT?TTT?ACC?CTC?CTC?GGC?AAA?GCA?GGT?C?3’

P10 5’GTT?GAG?CTC?GTG?AAA?CAC?AAG?CCC?AAG?3’

P11 5’GGG?TCG?ACT?TAT?AAG?CCT?AAG?GCA?GCT?TGA?CTT?G3’

Template is pig liver DNA, and tissue DNA extracts test kit and extracts.5 ' end probe PCR amplification condition is set at: 94 ℃ of pre-sex change 5min; 94 ℃ of sex change 30s, 60 ℃ of annealing 30s, 72 ℃ are extended 20s, 30 circulations of increasing; 72 ℃ are extended 10min then,, 4 ℃ of preservations.3 ' end probe PCR amplification condition is set at: 94 ℃ of pre-sex change 5min; 94 ℃ of sex change 30s, 60 ℃ of annealing 30s, 72 ℃ are extended 18s, 30 circulations of increasing; 72 ℃ are extended 10min, 4 ℃ of preservations then.The fragment that obtains is used for the probe of library screening after sequential analysis.

Primer P1, the P2 ATG upstream 1382bp sequence behind the 5 end control regions that is used to increase makes it to be connected to 5 complete end control region 7283bp with first half, and has introduced Bstp I site at the C of P2 end; P3, the P4 sequence between the 1st intron and the 3rd intron that is used to increase, 3 end homology arms as one of them gene targeting carrier pHA1, and, introduce the HindIII restriction enzyme site at the end of primer P4 at the end introducing Kpn of primer P3 I, Xho I restriction enzyme site; Primer P5, the P6 fragment about about 2.9Kb behind translation stop codon that is used to increase as the 3 end homology arms of another targeting vector pHPA2, is introduced Kpn I, Xho I restriction enzyme site at the C of P5 end, introduces the HindIII restriction enzyme site at the C of P6 end; P7, the P8 human serum albumin gene cDNA total length that is used to increase is introduced Sal I restriction enzyme site at the C of P8 end.

The mark of 3 radioactive probes and library screening

With random priming marker DNA radioactive probe (Random Primed DNA Labeling Kit, Promaga).

Get the dna fragmentation of 25ng purifying, add water to 30 microlitre of nuclease free, place 100 ℃ 5 minutes, placed cooled on ice again 2 minutes.Get 1 μ l mark object point to nylon membrane, treat to measure intensity of radioactivity after the film drying, use 0.5mol/L Na ₂HPO ₄ Wash 3 times, treat to measure intensity of radioactivity after film is done, calculate labeling effciency.The probe that mark is good places 100 ℃ to boil 5min, behind the cooling 2min, can add in the hybridization solution and hybridize in the ice.

Adopt benton-Davis hybridization and PCR to replace method for screening and obtain goal gene.

Get 1ml SM in the Eppendorf pipe, add a chloroform.The film that flushing is good is corresponding one by one with film and phage flat board, thrusts the plaque selected with micropipet or pasteur pipet until bottom glue, aspirates gently, in plaque and bottom glue tail pipe in the lump, moves in the ready Eppendorf pipe.Place 2h at least for 4 ℃, phage particle is diffused out from agar.But phage particle prolonged preservation and non-loss of activity in the SM/ chloroformic solution.

With reorganization lambda bacteriophage dna in the plaque is template, carries out PCR with primer D123 and D61 and identifies.The PCR condition is 94 ℃ of pre-sex change 5min; 94 ℃ of sex change 30s, 60 ℃ of annealing 30s, 72 ℃ are extended 20s, 30 circulations of increasing; 72 ℃ are extended 10min then.3 ' end probe PCR amplification condition is set at: 94 ℃ of pre-sex change 5min; 94 ℃ of sex change 30s, 60 ℃ of annealing 30s, 72 ℃ are extended 18s, 30 circulations of increasing; 72 ℃ are extended 10min, 4 ℃ of preservations then.

The positive plaque that selection is identified through PCR is spread the big plate of system again, uses 3 ', 5 ' end probe to carry out second as stated above respectively and takes turns hybridization.The positive plaque that filters out carries out PCR with primer D123, D61 and D81, D82 respectively to be identified.Through two-wheeled hybridization and PCR screening, filter out positive colony and bed board and preserve.

The acquisition of 5 pig albumin, 5 ' ending regulating sequence and 3 ' ending regulating sequence

Obtain before the PSA translation initiation site ATG fragment of 7kb behind the 14kb and ATG altogether through twice hybridization, two bar segment are intersected about 2.462kb.Extract lambda bacteriophage dna according to a conventional method, cut 4hr through 37 ℃ of enzymes of Xho I.Enzyme is cut the product electrophoresis and is reclaimed, and is connected with the pGEM7zf plasmid, and Transformed E .coli DH10 β is used for sequential analysis.The 5 ends for the first time dna fragmentation that obtains of screening by hybridization are cloned into pGEM7zf, called after pT5.1.The cloned plasmids pN51.2 that the second time, screening by hybridization obtained, cut pN51.2 DNA with the ClaI enzyme, reclaim the 6.3KB fragment, be connected to pGEM7zf, be built into the PN52 plasmid, with synthetic primer P1 and P2, with pT5.1 is template, through pcr amplification 5 control region latter halfs, 1333bp has introduced BstP I site in reverse primer altogether.Owing to Pac I site is arranged at the segmental 5 ' end of PCR, 5 ' end at pN51.2 has restriction enzyme site PacI with the pT5.1 lap, by Pac I site the PCR fragment is connected with pN51.2, forms novel plasmid, called after pN53, Fig. 1 are recombinant plasmid pN53 building process synoptic diagram.Cut the pN53 plasmid DNA with SbfI and Bstp I enzyme and identify, with the big or small the same (see figure 2) of calculating.

6 human albumin cDNA obtain

The product of recovery through about the human albumin cDNA PCR1.8kb of BstPI, Sal I digestion is connected to after the PSA regulating and controlling sequence, and regulated and control by it, and it is 7283bp that the plasmid pN53-HA after the connection contains PSA gene 5 end control regions.

The people cDNA that preserves with this chamber is a template, and P7-P8 is a primer, amplification HSA coding region.With the Sal I site of BstPI restriction enzyme site and introducing after the initiator codon, it is connected on the pN53 carrier with same enzymic digestion, PCR and BstP I, Sal I double digestion are identified, the structure carrier called after pN53-HA of institute.And behind the people cDNA of pN53-HA, connect the polyA signal sequence of BGH, called after pN53-HA-PA (see figure 3).Cut pN53-HA with Sal I and Bstp I enzyme, observe clip size (Fig. 4), cut with ApaI and SacI enzyme and identify the pN53-HA-pA (see figure 5).

The structure of 7 kinds of targeting vectors

7.1 the structure of pPHA1

On the basis of pN53-HA-PA, make up pPHA1.At first, use primer P3 and P4, clone the 2.8kb fragment at the enterprising performing PCR of pig albumin gene group DNA that obtains, i.e. fragment from 1 intron to 3 introns, when synthetic pcr primer thing P3 and P4, introduced Kpn I and Xho I restriction enzyme site respectively, connected carrier, be called pN3-1 in T.Change the ploxp-neo-tk of structure with Xho I, Kpn I double digestion, reclaim the neo fragment about 1.8kb, and be connected in the pN3-1 carrier that Kpn I, Xho I enzyme cut, constitute pN3N1, Sal I, Xho I enzyme are cut evaluation.PN3n cuts, mends flat through the SalI enzyme, the SacII enzyme is cut, and reclaims the purpose fragment 3n1 that contains the NEO resistant gene, and pN53-HA-PA cuts, mends flat through the EagI enzyme, and the SacII enzyme cuts back to close, and, is connected this carrier called after pN53-HA-PA-3n1 as carrier with above purpose fragment.With BamHI and HindIII double digestion pN53-HA-PA-3n1 plasmid and carry out terminal smoothing, electrophoresis reclaims the tk fragment of about 2.9K, Not I enzyme is cut the pN53-HA-PA plasmid, mend flat, dephosphorization is connected with the tk fragment, and the HindIII enzyme is cut evaluation, plasmid called after targeting vector 1 (see figure 6) that makes up, its target practice sequence is seen the target practice sequence of sequence table pPHA1.

7.2 the structure of pPHA2

PPHA2 just 3 ' section different with pPHA1, BGH polyA signal sequence was all identical in the past.Kpn I and Xho I restriction enzyme site in primer P5 and P6, have been introduced, sequence behind translation stop codon of pig albumin gene is carried out PCR, obtain to contain the fragment 3.0kb of Kpn I and Xho I restriction enzyme site, be cloned on the T-carrier, called after pN32, the construction process of other parts is the same.Be built into pPHA2 carrier (see figure 7), its target practice sequence is seen the target practice sequence of sequence table pPHA2.

7.3 the structure of pPHA3 carrier

We have cloned the HSA genome sequence, and 23kb has been connected on the pGEM-3Zf plasmid altogether, form pGHA, and the Sma I place in pGHA introduces the neo gene, is built into pGHAN.In primer P11, introduced Sal I, carried out PCR, obtained HSA genome SacI place, be connected to pGHAN, formation pGHANS after cutting the PCR fragment with SacI and Sal I enzyme to the translation stop codon fragment of 1610bp altogether with primer P11 and P10.Cut pGHSAS with BstP I and Sal I enzyme, to contain the 1.86kb that human serum albumin encoding sequence and intron I be inserted with the neo gene is connected on the pN53, be built into pN53HA, then with the sequence PCR fragment behind translation stop codon of pig albumin gene, and the 6kb that is connected with the TK gene behind 3 ends is connected to pN53HA, be built into targeting vector 3, i.e. pPHA3 (see figure 7), its target practice sequence is seen the target practice sequence of sequence table pPHA3.

Pig serum albumin gene group gene order

SEQUENCE?LISTING

＜110〉Biologic Engineering Inst., Academy of Millitary Medical Sciences of P.L.A

＜120〉be used to produce the pig serum albumin gene seat targeting vector construction process of foreign protein

＜130〉sequence of pig serum albumin gene group gene

<141>2004-06-06

<160>1

<170>PatentIn?version?3.2

<210>1

<211>35316

<212>DNA

<213>porcine

<400>1

ttctgagtca?caacaggaac?tccaactacc?caacattctg?ggataatcta?tctggggaaa 60

aactctgaaa?gagaatgaat?gtgtgtatat?gtataactga?atcactttgt?tctagagcaa 120

aaattgtcat?aaccctgtaa?atcaactata?cttcaataaa?actttaaaat?gaaaaaaaaa 180

attacatgcc?aggaaggtat?agattacttg?tgtgtctctt?aaatttgtta?gagatggata 240

tttaatggca?ttccttagtt?tttttcccac?catataatcc?ttttgcacat?ttttactcat 300

taattttaat?taacgggttt?actaactctc?tactcggtgt?ctagtactgg?actgaaattt 360

tgtggaaagt?acaataaata?agacaaattt?ttactctctt?tttttttcct?ttcacattta 420

aacatatttc?attggctgag?tgtaaaggta?aaagccaagc?tttacagaac?aaccattaga 480

agtatacagt?ggatcccttg?tagaaatcat?gaaggaaagc?cagtgtaggc?aatgtctgga 540

gcacaattgc?aaaaggtgag?ttgttttctg?tgatggaagc?agagcaattg?tcaagagttt 600

gactggttgc?aaaagaatat?aaagggccag?ccaatgttta?agaatacaca?atatggcttt 660

gatgtactcc?tgttacattt?actgagaagc?cacacatgct?acatattaag?caatctatag 720

aattcctctg?agtccctatc?cagtgattgt?atttccaaat?gagataagta?ggtgagatat 780

atagacaaaa?tctgaaggct?tgtttagtgc?tgctcttcca?tcagtctcca?aggtagccgg 840

agcattagta?cctcacttca?aacattttgc?taagaaaaaa?atgaaaataa?gtaaaaaacg 900

tgggaaaagg?ccataatttt?ttggaagccc?tctagaacaa?actgtgcaca?gctaatcaga 960

tacacttctg?caattctgtt?tccatgcatg?agtagagcta?caaatcttta?ctcttgtaaa 1020

ataattataa?tttttggcag?ttcccacaca?gcacacagac?acggctctga?tctggcattg 1080

Pig serum albumin gene group gene order

ctgtggctgt?ggtgtaagcc?agcagctaca?gctctgatta?gacccctagc?ctgggaatct 1140

ccacatgccg?tgggtgtggc?cctaaaaagc?caaaaagaaa?ataatcataa?tttttaaatt 1200

aaattgttac?cgtaaaattc?aagaagtatg?actaagaact?aaaacaaagt?aatagacgtt 1260

atcagaagag?acattatcac?aagactgcaa?ggctgggaga?gagaatgttc?tgagatgtgg 1320

tctgagggga?ggaggatttc?tataaatgga?aatcaggggg?aagtcatcat?gatgggagat 1380

cacctaataa?gcagaaaact?tggtctggaa?atgaacttgg?tatgctaggg?aagatggatg 1440

tgaccttgca?gctccttgct?cagtcttctc?acctatcatg?ccattctatg?ctttgcaggg 1500

ttgcaaaaaa?atgttacact?gagtgttact?gacttaatag?gggaacttat?gcgtgaaaga 1560

gaaataaaag?attatgctac?agaaatcatt?ccttcagtta?tgtgttcatt?tatttactca 1620

ccccactcaa?cgaatactga?atatatgtta?tatgccagtc?atgtgttatc?catgggaata 1680

ttgcaatgat?ggagaaagat?taaagcctct?gatttcatgg?atgttataaa?cgggtggaat 1740

ctgattcctg?aacattttga?ataccagaat?aaggagattg?gaatccattt?aaaatacaat 1800

gggggagcat?taaaaatctt?taaggagagg?aaagttttag?gattaatcag?agtatataag 1860

gacttgaggt?atgaacatta?attaagagtt?tattgttaaa?aaaactcaaa?caaatggagt 1920

tccctggtgg?ctcagtgggt?taaggatcca?gcattgtcac?tgctgtggct?taagtcactg 1980

ctgtggtgtg?ggttcaatcc?ctggcccaag?aacttccaca?tgctgcagca?cagccaaaaa 2040

aaatttacta?cttttttaaa?aataataact?catccatgat?ttattggtta?gtctgaacca 2100

gagttgtgcc?agtgtttagg?ggaatgtggt?taaatgtgat?atttttaaag?gatggatcat 2160

gattatttag?gggcagattg?ggtacttgag?attaaggaga?aggaggtatt?aaaattacta 2220

tatggttata?aaaatgagtg?gcatggagaa?tcagagacta?ataagccaaa?cgtaaatttg 2280

gaaaaaaaaa?tagatgaggg?tcgtggtggg?aagatgacaa?aatgattatg?aatatactga 2340

catactgaca?tactgggttt?atgtcaagag?gattttaaga?ggaggactag?aacttcaagt 2400

gttttgacag?tgaccacact?aagaaatata?tcttacgtaa?tgaactaata?tagacaaata 2460

tagaaagcat?ttcgctaaat?gattatttac?cctactataa?tgtagtgcac?tctgatcaat 2520

tctgttcatt?ctattttact?aaaaaatact?ggtaatgatg?caaaatttat?gttgtgaccc 2580

tgtttaaaaa?aaacgttcta?aaggtagttg?aaaatcagga?ccttattgta?gttggtgagt 2640

cgtagagatt?tttggcaaca?aggaacaagc?taatatagat?tttgaactgt?gctatgaggc 2700

tttaggaaat?aagttcaagt?ccacagggta?tagaaattct?tcctaaatga?tcatggccac 2760

Pig serum albumin gene group gene order

aaaaactgag?actccccaac?acatgaatta?attagttaga?cattcctagg?gtgtagataa 2820

caaagaatat?tagaaataat?catttttcta?taaaaacatt?ttatttaaca?aaaaggatat 2880

cagtattaac?atctgactat?acacaagaat?ttttttcaat?tgataaggaa?tgttacttat 2940

atcttccctt?catcatccct?tcagacacat?tccatatttg?aattttccca?ggaatatact 3000

cataatttta?aggtatagtc?aaaatgaacc?aaaaagtgac?ctttgaatgg?actgctttag 3060

gttatggtag?ctgtcagttt?aacctgactg?atgaagaagc?aatagcacct?atgcagtaag 3120

tggccttgag?caagtttttc?tacagatggc?cttgcttctc?agggaggttg?agtcattgga 3180

aagattttcg?tacttgaact?tatccagctc?cccctttgtt?cactcaccta?aactgcatat 3240

gaaagaatat?aaatgggaat?tagctttggc?ttggggcttg?aatttgggtg?gggaatgcaa 3300

ggaagtatca?gccatatggc?agcagactag?gaattgagca?aaattctggg?caatgagtat 3360

agctgctggg?ccaatgggat?atatgctggg?atatttaaat?agatctctgt?atgcctaatg 3420

gaaagcatgt?tttagcttct?ttaattggtc?aggattgaag?taagtcagct?tctgttaacc 3480

tgtttgcctc?cattttatct?ctctaactaa?actaattccc?aggttgcact?ccactgagaa 3540

attaaacaat?ttaattgtta?tgtcctgggg?aggacgtata?gtatcaaaat?caaggtgctc 3600

atgtcctctt?accacatgga?tgcaggtgac?tcttgctcag?tcaaattaaa?ctgtcaaagt 3660

tttgaatggc?ttccatatct?caggcatgcc?ctggatctgg?cgttagacat?agcagcgata 3720

atacatggat?tatgccttta?aggaccttat?tgcaatgggg?ctatcagaca?taaattactg 3780

catttcaatt?gtaaggaaac?attgatctaa?caaccttaat?gatccgaaaa?gggaaaaaga 3840

gaaaaataag?tttctgaaca?accagataga?gattccaaag?ttcatacacc?aaagttcagg 3900

ctttaaagtc?tatttacaag?ggtcaattaa?cctctgtcct?agtaggacag?gacctacatg 3960

gtgaatgttg?aataactatg?gaaacacagc?ctcaggttct?gcttcttcac?ttggcttgag 4020

attcactgac?agaatagcca?tccttctaaa?atgcttttat?ttctaagaag?ttgaagtgtg 4080

gttttgtgga?ggaaatagag?tattgaggaa?attgagagga?gctgaggtaa?agacagactc 4140

agattttgtg?gagttggttt?tataagactg?ttgatcttta?acatccacct?gaatttgccc 4200

acaaattcag?acatgacagt?tttggcatgg?ggtgtatatt?agacaggagg?cacatcaagt 4260

agaggtaggg?tgccagtgga?ggcttaggtg?cacccaagag?aaaagcaatg?ctgtggtgca 4320

ttgaagacat?gcatttgaga?tgtgcagagg?gcagtagaat?gggagagagg?cttgatcatg 4380

agccctgggt?acaggttgga?gagggagatg?agtgccacag?atatagactt?tcttactttt 4440

Pig serum albumin gene group gene order

cagtttagcc?caaggtaata?cgtctctctg?ctcggtgcac?atcctcattt?ttatgactac 4500

ctcaactttc?tctattatcc?tcaaatattc?agctccaagc?tcttcattct?tagcaatccc 4560

tcccgtatag?aaaaaaataa?aagtcacaat?cagtggaacc?ccttgtcact?atcttctgaa 4620

tgctcatcag?tgaaaatttt?caccgatcct?caaccctctt?catttgtcct?ccttcatatc 4680

cacatttctt?ggaacgagtt?ctccataccg?catgtactta?ttcatcttcc?atttatttct 4740

ctggccacta?tagtctggtt?tttggtctca?ttatcccatc?agatgctcta?ttaaggacat 4800

tagcaatgcc?cttgcaacgg?taattacttc?aattctattt?accatcgctg?tcagtgatat 4860

ttgtaacact?ttacctattc?cactcgaaca?tttctcttcc?ctttcattcc?ctggaataac 4920

actgcttgat?ttgtctcctc?ttctctggcc?acccgttctt?tggtctctga?tctttccatt 4980

ccatgaaggg?cctctccttg?tcccatgtta?ctctcttccc?aggctatctc?catcttgtac 5040

ggcttgatga?ctcacaaata?atattccaag?cttagacctt?gccactgacc?tctaaactct 5100

acaactatct?ccttgacatc?tctttttttt?ggcaatgttc?ttttgtttaa?aaaaaattat 5160

tgagttatat?ttgatttata?atatgatatg?tttcaggtgt?atgatataat?gatccacgat 5220

gttaaaagat?tttactccat?ttatagttat?tataaaatat?tggttatatt?tctgacactg 5280

tgtgatgtat?ctttatagct?tattagtttt?atacatagta?gtttgtacct?cttaatcccc 5340

tgcccttgac?ttgcccttcc?tcccttccct?ctccccactg?gtaaccacta?gttttgttct 5400

ctatatctgt?cagactgttt?cttttttgtt?atattcccta?tttttttttt?tagattccac 5460

gtatgtggta?acatacagta?tttgtctttc?tatgtctgac?ttatttcacc?aagcataata 5520

acctccaggt?tcatccatgt?tattgcaaat?ggcaacattt?cactcttttt?tttaaattta 5580

attttgatgt?tatattggag?catagttgat?ttataatgct?gtgttagttt?caggggtata 5640

gcaaagtgaa?tgttatacat?atacatatgt?ctattctttt?ttaaattctt?ttcccatata 5700

cgttattaca?gaatgttgag?ttttttgtgc?tctacggaag?gtccttcttg?gttattaatt 5760

ttatatacag?tactgtgtat?atgttaatcc?caaattccta?aattatgtcc?tgccccaacc 5820

tttccccttt?ggtaaccata?aatttatttt?tgaagtctgt?ttctgttttg?taaataagtt 5880

catttttatc?ctttttttag?attctacata?taagtgatac?catatgatat?ttgtctttct 5940

ccgtctgact?taacttcaca?tagtatgata?atctttaggt?ccatccattt?tgctgcagat 6000

ggcatttcat?tcttgtttta?tgactgagta?gtattcgtgt?gtgtgtgtgt?gtgtgtgtgt 6060

gtgtgtgtgt?gtgtgtgtgt?gtgtgtgtgt?gcagccaaca?gtgtataagt?atttcctttt 6120

Pig serum albumin gene group gene order

ctccacatcc?ttgccatctg?ttttttgtgg?tattttaaaa?tttttccttt?agggctgcac 6180

ctatggcata?tggaagttcc?cagactaggg?ttccaattag?agctgcagct?gttggcttac 6240

accacagcca?cagcaacacc?agatctgagc?cataacttcg?acctatgttg?cagctcacga 6300

caacaccaca?tccttaatcc?accgagtggg?gccagggatc?aaacccatgg?atactaattg 6360

ggttcatttc?cgctgagcca?caatgggaac?acccttgtgg?tctttttgat?gttagccatt 6420

cagataggcg?tgaagtaaaa?tctcattgca?gttttgtttc?ctgatgattg?acaatgttga 6480

gcatcttttc?atgtgtctgc?tggccatctg?ttatgtattc?tttggaaaaa?tgtctattca 6540

ggctttctgc?ccatttttta?attgaatttt?ttttggtatt?gagttgtata?agctatttat 6600

atattttggc?tattaatccc?tcattggtca?tatcatttgc?aaatattttc?tcccattcag 6660

taggttgttt?tttttgtttt?gttttgtcga?tagtttcttt?cgctgtgcaa?aagcttttaa 6720

gtttaattag?ttaattttgc?ttttgttttc?tttgccttag?gagatagatc?ccaaaaatat 6780

tgccacaatt?tatgccaaag?agtgttctgc?ctatgtttcc?ttctaggagt?tttatggtta 6840

ctggcctcac?agctgggata?aaatgtagct?ggaagaagcc?agagggggcc?ttctaaagca 6900

tggatttcag?agaaggggct?cccttgcttg?ggtccaagac?cagtaccctc?ctaaaaagct 6960

cttcaatctg?tccattcctt?catttctata?ccatcgatat?aactcaagat?ctcaccatct 7020

gttgctagga?atattgcatt?agtgccctga?ctagtcttcc?tagacatact?cttgccttct 7080

ctagtctctt?ccccacattg?caaccagagt?agtcttctaa?agtatagatc?ggattatatt 7140

attgggctaa?taatatcatt?caacttctca?atgcttttag?aatgaagacc?aaaaatactg 7200

ctatagctaa?gatgacttcc?aaatccatct?tctctcatct?cacactgtgc?ttctcctcaa 7260

ctctgtgtct?agctggtctc?agtttctttc?attctctttc?ttttctcaag?cattagcaca 7320

ttctttcccc?tgtgcttgta?aggctatttt?ctttcatctt?tctttatgcc?cattttaaaa 7380

agtctcagtt?tagaagtcat?gttcctagga?aagccttctc?agacagactt?ccaggcacat 7440

ggtattatta?aataaatatg?gcttgaataa?aaaacaagta?tttatcacta?aatacttggg 7500

tggcctactg?actttgtcag?tcctctcaag?tgaactattt?ccctataaaa?gttttgtagc 7560

tttctgcaag?aatatgtttc?tttgggattt?ataatattaa?gatgacctat?ctcatcctta 7620

actttgcctg?caaatccaaa?taattgcaag?ttctgctttt?ctattgctga?ctgccagcct 7680

gcctttcttt?tctttcttac?tcctgcagga?aatttcattg?tcttctaaac?tgaagtctaa 7740

ctctttgctt?gctgcatttt?gatcttttct?caaacattta?gaaattacac?atattcatac 7800

Pig serum albumin gene group gene order

cctccttact?tccctcaatc?ctctacctac?tcattccatt?tcttcaggtg?acaattcctt 7860

cacaagcaaa?aatgaaccca?gagcatgcaa?ctttcaagct?tttgtctgtt?aagttgcata 7920

gttagctgga?gtttagagcc?cgtatatctt?atttgtgtgt?gagattgtcc?ttggatttag 7980

agcttctcct?ctgatacttt?tttatcaccc?caggagtcct?ttgtgttcta?catgcacagt 8040

actttacaat?gttgtattgt?aactatcttc?cagtgtgtct?atcttccccc?tattaaactg 8100

cctgcttcat?gacctatgga?gggcttcaag?aacttaagag?tcagggactg?tgtcttgttt 8160

ttattgtttt?taacctcaca?tcctaggaaa?tgcttgggga?gtgagcaccc?catacatttt 8220

gagcatgaat?gaaggggtag?gtggctactt?aaaataataa?gtctgaaaca?ttgtttcaga 8280

actgtgcttg?tcagcaagaa?cgctgatgtt?cctaagtaaa?tagtgctttt?gttgatgcca 8340

ggacaatgtt?tgcttttaga?taccatgatt?ctcaatgtgc?attttttgcc?aattttccac 8400

gtaataatga?agagaaattt?gaactcatca?ataacggtca?tttgcattca?aggcattaaa 8460

tgctgagagg?ctaattttta?taattttaat?gaatgaatga?atatctaata?taaatataaa 8520

aattaaaatc?gaattgaaat?aataattgta?tatatttcat?ctaataactt?aaactatttg 8580

tattaaataa?tctaaatatt?tcacaactgt?gaatcagaag?cgttttggaa?agagcatgcc 8640

attttgtcac?attatatgct?gctgaaatta?cttcttcctg?ctgtttgaaa?gcatatgttc 8700

atttcaacag?gttattacca?aatagctatt?tttgcaaatg?gaaacttcaa?gcctctaaat 8760

attattctaa?taaaagaatt?ttgctctttt?aaatgtctac?ataatgaaaa?actaatagtg 8820

aacaattcat?ttcttatatg?tgcaataatt?atatttaaga?ccatttcaaa?aatatttata 8880

ttgtatactt?tattacatta?tataggctag?acttggattt?ctcttaaaaa?aagaaaatta 8940

tgccaattat?ttgtttgata?tatttcttaa?aagttacaag?tttctttgga?tcaaataatt 9000

catttcttga?tattagcccc?tacatagatg?cttgaaaagc?tgatgagaat?tttaagactt 9060

aaagtgtaag?gctcaggtgg?ggggcaggcc?agagaagttt?tagttgaagt?ccatcaatgg 9120

gaggttagaa?aacatctgat?aaatttgaag?ctttagaaga?catcaaggag?aaaaaaaaat 9180

taccctttta?tgaaaacttt?ctgaatttta?tcaaccaatg?tttttaatat?aggactgttt 9240

tagctacaaa?acatttgctt?ttttacaaga?ggttaaaaat?agttccattt?gtaactaata 9300

tccttttaag?taataagtcc?cttgaattac?atttggaaag?aaatttagaa?tatgaagaga 9360

ctttttatta?tattattata?ttaaggacaa?ttaaaccccc?agtaatgtat?taaattctgt 9420

ttgatttcca?tctgttgcta?ttattatgaa?agagaagtta?ctagcattaa?tttcattttc 9480

Pig serum albumin gene group gene order

tatgtttgca?gactctgttg?ttaacttata?caaagaacag?ataacatgga?tttcaggcat 9540

cagttatatt?tttcttggat?tggaaaaatg?ataaaaagat?atgagtgtta?ttttatttct 9600

ataatttaga?gaagtgaaat?gacctgttca?tttcttaaga?ctcttaacct?gaagtagata 9660

aagagtgatg?cctaaggaac?tcatgggcag?ctcagcgatg?aacagattcg?cagatctgga 9720

tcccaattcc?ttaacttgat?atagtcattc?taccagtcag?gccctttact?cattctatga 9780

ctgtttcatt?gggaatatgg?aatcacagaa?tgttcaaggc?agaagtgtcc?ttaaagatta 9840

tttaatccag?cttgacccat?atggcctaaa?gatatagaca?gatctgcaca?cttgagtttg 9900

cagctagaaa?ttgacattga?gatgaattaa?actgcatagt?aaagagttga?gaagttttaa 9960

gtaaatctga?gagaaagtag?acctaaattc?tagatgccca?gtgtagcatg?aggaaagatg 10020

gaaaagggac?cctcctgcca?tgggcgcttt?ggagagatct?ggatttaaga?gatgggacaa 10080

gccaaggatg?gtaatttagt?gtggttttta?taatgtaaac?tttcttccct?ttatccccta 10140

tgattttttc?agcaaagatt?tttctcactg?ggattcctgc?catggcacat?tggaaatgaa 10200

tccgactagg?aaccatgaga?ttatgggttt?gatccctggc?ctcgctcagt?gggttaagga 10260

tctggggttg?ccgtgagcta?tggtgtaggt?tgcagatgtg?gctcagatct?ggcattgctg 10320

tggctgtggt?gtaggccggc?agttgtatct?ccgattagac?ccctagcctg?ggaatcacca 10380

cgtcgcgggt?gtggtcctaa?aaagacaaaa?ggccaaaaaa?aaaaaaaaaa?aaaaaaaaga 10440

tttttctcac?taatgcaagt?gtttatggag?ggctgtgaag?ggacaattga?tatttgaatt 10500

acattacaaa?ttttatgttt?gcattagaaa?tgcctatatt?gttcacacaa?tttggacaat 10560

ttattttctt?ttattaatgg?gaaatgtatc?ttcataagtg?aataagaaat?taaattgctt 10620

accatatttc?aattagtttg?atgcctaata?ctctttcact?tttctatcct?tctgtgtttt 10680

tagaaagcag?tccaggctct?tcaatgataa?ataagatcta?tcctacctat?atgtggacat 10740

ccacacatat?gtcccatgca?taataggaag?aatgttaaga?aacgattttt?ctgtttaaat 10800

ttccctccaa?ataaatccaa?atttcccaca?ttttggagtg?actttgggtt?aaatgtttct 10860

agaaagtctg?tgttgtaata?ctgaggctat?tatcagtcat?tgttgtccca?ttttaatgca 10920

tatgtttccc?ttgatttcta?actgaaggca?acccagaagt?aataatctat?tacagaagga 10980

gatggcataa?aggttgaaga?aggaaaatgg?acaaatggag?ggaaacctgg?tttgaatgac 11040

ttggtggaag?ctgcagggat?cccgaaggta?gcaggagggt?ataggtttaa?gtttttgatt 11100

aggatggcaa?ggaagtgggg?gactggtcca?agagatactt?catctgcttc?aaatctgtgc 11160

Pig serum albumin gene group gene order

cttttctttc?ctgtcaaaat?gaatgcaaaa?aatgaaggac?aaggggataa?aatatttcaa 11220

ctgaggtatt?tttttctctt?ctaaatgatc?agcatataat?taactctttc?cataagaagt 11280

aatcttctta?actactctct?ctttaaactt?ctcccataaa?attaggaaca?cattgaaaaa 11340

ttattggagc?tagtttccta?tttatttaga?agtagtttct?ataagtcata?tttaaaactc 11400

ttggtttaaa?tattcacatt?tactcttgtg?ccagattcct?taggtgacta?actaaataca 11460

agggtttaaa?atcttgaaga?acagaatact?tagaaataaa?caacaattaa?gtaattttat 11520

tcatctcagt?ttagaatata?tactcagaat?taatagaaga?aaaatgtagg?tacccttact 11580

ttgggaacct?acaagatgca?aatattcaag?ggtttcttaa?aagttaataa?ttttaaagca 11640

gatagtgtta?agaattaaat?attttaattt?ctcaaccatc?tgctaatctt?taaaaaagat 11700

acatacacag?aaatttcatt?atgctattca?caggaaacat?tcttttttaa?aatgagtttt 11760

attttttcca?ttatagttgg?ttatagtttt?ctcctgattt?tctactgtac?agcaaagtga 11820

cccagtcaca?catacatata?taccttcttt?ttctcacatt?atcctccatc?atgctccatc 11880

acaaatgact?ggatatagtt?cccagtgcta?tacaccaggc?tctcattgct?tattcattcc 11940

aaatgcaata?gtttgcatct?attaacccca?gattcccagt?ccatcccact?ccctccccct 12000

cccccttggc?aaccacaagt?ctgttctcca?agtccatgag?tttcttctct?gtggaaagtt 12060

tcatttgtgt?cgtatattag?attccagata?taaggaaata?acctttcagg?tgttttcagt 12120

tttatatttg?aaaactctgt?gatcttcatt?aaatataaga?atatcaattt?taagtttcta 12180

aaattgtaat?gaatttgatt?tggggggttt?tctgtattta?ccattttatt?acaattgcag 12240

tgcaaagtag?gtggagagac?taatattttt?cttttcattt?gtcaaataaa?aaatatgcac 12300

gaagtatatg?tgtgagtgac?aaaagcctga?ttctgtgaat?tagtaataca?gttatatcca 12360

aggataagga?ctgcgtattt?aataaagcct?tcccagagga?ctcatgagga?acactgactt 12420

caaataaaag?atgccttttt?ctcagaagct?caaagttgat?ataatttttt?tttaaattta 12480

gaaaattaga?atagctgcct?tttttctctt?ctagtattgg?attttaggct?ttaaaaaatg 12540

attttctcca?atggaaaaaa?ttccatagac?agacattcac?ttatttctgt?tcagctgtgc 12600

ccagaaagac?aagtctattt?ctgttattta?tggagccatt?aaaaaaaaaa?aatctatcag 12660

ctctgtctag?ctaggtagct?aatcatctat?ctgtttgtat?tatctacctc?tgtaatctat 12720

caaaatattg?tcttattgcc?tgctttccta?tcatctcctc?ttttgtgctt?gactatttaa 12780

ttagtaccca?tacctgagaa?gtgaaaaaaa?aagtcataga?aaataaattt?ctttcatcat 12840

Pig serum albumin gene group gene order

atgactgaat?aggaaaaata?atacttcttt?taagtattta?cttgtgtctg?tgccatatgt 12900

gccacagagt?gacatggctg?acagtttgat?tgacttggtt?atttaattaa?ctgaaatgac 12960

tggcttgata?ctaattattg?ttctgtagta?tcaaagtatc?agaggaggtt?agttttcctt 13020

tttcttttga?ttgaaatccg?tagttcttct?tggctgtgtc?gatgacatgt?ggaagttcct 13080

ggaccaggga?tctaacgggt?gctgcagctg?caaccagtgc?cacagctgtg?gaaacgcctt 13140

aacccattgc?accaccaggg?agcttcatga?aatccatagt?tcttcatctt?tagagcaaaa 13200

gggcagagag?agaatagtcc?ttcagaggca?gagatcagaa?tttggggtgg?gaaaagtaag 13260

gaaggaagtt?cgaaagaaca?tttttgaaaa?attatctttg?tttttagaca?tgaagttaaa 13320

tttatttcga?aatttaaaat?aacattgaag?aagagcatgt?gactttcctt?tttagtaaaa 13380

gcgagaaaga?aagaaaaatg?tgaaataggg?atggaaagaa?tataatgcct?ggtaaagaag 13440

gtctaggatt?cttaaaacca?ctaatataat?caggctcagc?ctgccctgtg?tatatcatga 13500

tagaaaagat?taccactgct?gccattgtgt?ccattttctt?ccccatatct?ccatgagagg 13560

taccatactg?cctttaagta?ctttactcta?gagcctgaat?caccagtggc?cagagctgag 13620

gtaaaaatgt?aggacttctc?aacatccgtt?ttagttgtta?ttgttgctat?gactttttct 13680

tagtttgcat?ttgagacact?ggtttttttg?acagaacagt?ttcagaagaa?tgaaaagcca 13740

tggaagccct?cgaatgcgtg?catgcatgca?tgcatgcatg?cacacaccca?tgtgtgtatg 13800

cattttcact?acttatcaat?aaaagcatac?ttggaattaa?taatagatac?aattcagagc 13860

ttagctcttt?taacatgtct?tatttccaag?ctaagtcact?ggtttgctta?aatttttgca 13920

aattttgaaa?actaacacgt?tcaaatggta?aaccacagat?agagatcatg?gtaatagtac 13980

aaatcgttta?aactcttatg?taaaatttga?taaggtgttt?tacataactt?taatgaattg 14040

acaaggtctt?gtggagaaaa?cagatccaga?cggcaaacat?acgcaaggga?tttagtcaaa 14100

cacatttttg?gcaaaaaaac?tatgaatttt?gtaatcagtt?gtgagccaat?gaaatacaaa 14160

aatgagtcta?gttaataatc?tacaattatt?ggttaaagaa?gtatattagt?gctgactttc 14220

ctctgttcgt?cctacctttt?ctcttctatc?aaccccacaa?gcctttggca?caatgaagtg 14280

ggtgactttt?atttcccttc?tctttctctt?cagctctgct?tattccaggg?gtgtgtttcg 14340

tcgagataca?tgtaagagtt?cgatttttct?attgttcaac?ttttattttc?ctagtaaaag 14400

aaagtttcag?taaactttga?atctttgatg?aattaagttt?tcctttagca?tttatttcta 14460

gactgttatg?atattttgta?tttgcgaacc?cttaggaagt?tatcttatta?atatgtttct 14520

Pig serum albumin gene group gene order

aacatgctgg?gtgaaaaaat?agtgagaatt?gtttagtagc?tataattttc?ttttgcacat 14580

gaaggagctt?atgtagagtg?acttacactt?cacagagtgc?agttgaagac?agaattaaaa 14640

attccaaatt?cctaaaggcc?tattcactgt?accttacacc?ttggtttata?ttaagaatat 14700

acaaacactt?tataaaaaga?atttgcattt?atatttttca?tttcagacca?tcctctcagt 14760

agctggtaat?agatactaaa?tgaacattag?atcttatcca?agtttgaata?gaaagccata 14820

aatatttaat?gattttaatg?gtttttcaaa?taatatcatg?ggcaattaaa?taactcttct 14880

gtttaaatgc?aaaagaagaa?atttaccacc?gtcctaagat?tttctgtaaa?gatcagagcc 14940

cagtatctag?gagcaaatga?atacctaagt?caaatgaaga?gaaacaaaaa?atattatctt 15000

aactggttgt?ttcgtcagaa?tttaagcaaa?actttttttc?tctcctcttt?tccccagaca 15060

agagtgaaat?tgctcatcgg?tttaaagatt?tgggagaaca?atatttcaaa?ggcctgtaag 15120

ttaaaatatt?gaagattcaa?atttaatgtt?tctattagtg?tcagttatta?ttttgacacc 15180

ctcatattcc?cccgccatca?gagattctag?agcagtccct?ccagtagaat?tttctgcgtt 15240

ggtggaaata?tcctctattg?gtgttgtctg?acagttgcca?ttggccacat?gtggctacgg 15300

agtactttaa?atatatatga?cgagtacaac?tgaggatcaa?aaatttaaac?agcatttact 15360

tttaatttat?ttaaatctac?atcgtcactt?atggctaatg?gcaactatgt?tggacagtgt 15420

agctctggaa?atcttgtata?gcctgcatgg?ggggaaggat?tttgctatgt?gattcgttca 15480

caactcgctt?tccctagcaa?atagttctgt?ggtttcttga?gataatttca?atttttttaa 15540

tctaataaat?agtgagaaaa?cagtttattt?ttcaaggtct?atctcatcct?acagcctgat 15600

taaatgagtg?tgtgtggttt?tttgtttgtt?tgtttgtttg?ttttaaatag?ttagcctagg 15660

taatgtccca?gtcttagtaa?ctaggtctat?tcgaatacat?tttacctgtt?tgctctgtgt 15720

gtatgcatct?tctgacatga?tacatcttct?tccatgatag?tggaaatata?catccttggc 15780

ttctgaagcc?acatgtactt?cgttaagcct?cagatcacag?ctaatgtgaa?caggctttct 15840

ggctaagagt?aagaaagatg?tgtaaaatat?agatatacag?acacacaatt?tgttttctag 15900

cttagcaatg?tactgtatat?taaaattttg?ttacattttc?tacgtccttt?gtttcagagt 15960

gctgattgcc?ttttctcagc?atctccagca?atgcccatat?gaagagcatg?tgaaattagt 16020

gagggaagta?actgagtagc?aaaaacatgt?gttgctgatg?agtcagctga?aaattgtgac 16080

aagtcaattg?taagctatat?tctgctttag?gatagtattt?cttctgcttt?tatgtaccct 16140

tgaacattta?aaagggaaaa?agtccccttt?tagttttctc?ggtgattact?aagtgaccat 16200

Pig serum albumin gene group gene order

agttgtgagc?aatcctcaaa?agtgggtaga?gccattattt?cttctgtcac?agagatgttc 16260

tagctataac?cacaggttgg?ttttgttgtt?gttgttgttt?gttttgtttt?gtgtcctttt 16320

agggccgcac?ctatggcata?tggaggttcc?caggctgggg?gtcgaatcgg?agctgtagcc 16380

tccggcctat?gccacagcta?cagcaacacc?agatccgagc?cgtgtctgtg?acctacacca 16440

cagcccatgg?caacaccagg?tccttaaccc?actgaacgat?gccagggatg?gaacctgcat 16500

cctcatggat?actagttggg?ttcattaacc?actgagccac?aatgagaact?ccccattcat 16560

atttttacat?accataaaat?atcaggactt?tgaacttttg?atattttcag?atagatctac 16620

aatatacggg?taagtttatg?tttctctcac?tgctttatac?ttagctttct?ggggacaagg 16680

caatttttta?tttatcactg?aaggattttc?ttctcctcta?tatgtacttc?ttcctcaagg 16740

cttttttttt?tcatttttta?aaagttttat?tgaagtagaa?ttgatttaga?atgttgtgat 16800

aatttctgct?ttacaacaaa?gtgattcagt?tacatatcta?ttcttttcag?attcatttcc 16860

catatgaatt?atcacagaat?actgggtaga?attccctgtg?ctatacagca?gaccccgttg 16920

gccagtcatt?tcatatacta?tagtgtgcat?ttaccaatcc?caaaccccca?gtctatctct 16980

ccctcacccc?aacatgtcct?ctttgggaac?cataagtttt?tcaaaatctg?tgagtttgtt 17040

tctgttctgc?aaataaggtc?atttttatcc?ttttttaaaa?attttaattc?cacataaaag 17100

tgatatcaca?tgatgtttgt?ctttcactgt?cttagtatga?taatctctag?gttcatccat 17160

gttgttgcaa?aaggcattat?tttgttcttt?tttatgggct?gagtaatctg?aatcagttct 17220

ttaactctgt?gcaataatat?ggctctaaat?gtgaatttta?aaaaatacct?tttaaaaatt 17280

aataaaatct?gtctagagca?gcttatttgc?ctaacattac?acttactttc?ccggtaaaac 17340

ttaatgagga?cagaaaggac?tcatttaatg?gaatgtgctt?tgaaataaat?atttctagaa 17400

ccaaaaaaac?ctatattttt?ctgtgataac?aatatatagt?ggattgcata?actcctcagt 17460

tttcaacagt?attaactaaa?aggctggacc?tctcaaaaag?gagcagataa?aatgagaaac 17520

tacctgaaca?ctcttctccc?taaattgaat?tcatctgcta?cttagagaat?tgtttttcct 17580

tgattttcta?ttatggtgat?tgctctccta?acctttagtc?tcaggtggaa?ataggagcta 17640

tgctcattcc?ttaaaaccat?ggagtttttg?cctttctgtt?ttatccacta?taagtaagag 17700

tgtgacagac?agttaattct?tatgaaaatt?acatatagat?ggtcaaatca?agggaaactc 17760

tctaaacctg?aaaaaaatgt?ggatttttat?atgatttcct?aaccaaggtt?taccaggatg 17820

ctgattttta?atactattct?ttgagtatga?ggaaaataaa?atgctgccag?caatctgaag 17880

Pig serum albumin gene group gene order

cctgctctct?ctttcattta?gcacactctc?tttggagata?aattatgtgc?aattccatcc 17940

cttcgtgaac?actatggtga?cttggctgac?tgctgtgaaa?aagaagagcc?tgagagaaac 18000

gaatgcttcc?tccaacacaa?aaatgataac?cccgacatcc?ctaaattgaa?accagaccct 18060

gttgctttat?gcgctgactt?ccaggaagat?gaacagaagt?tttggggaaa?gtaagtaatc 18120

agatttttat?gcttcaaaat?taaaaattat?ggataagtga?agactcagta?taacaaatta 18180

ctattaaaga?tatcaacctt?aagctttgaa?gcttttgttc?tgacaatttc?ctaggaaata 18240

atatatgata?atacaaaatt?ctacatgatg?aaaaaaaatt?gctcaaaggt?cttttgcagt 18300

tcttcaaagc?aggatatgac?ctgtatgaaa?atatttgaag?ttgacatgta?ttaaaatatt 18360

ataaatatat?acacatgtat?atatttagta?ttctcagaat?ttctcttttg?tcaataaaca 18420

tatcatcaaa?atgtaactat?tttacatgtg?tatatgtatg?tttaagttta?atcttttcac 18480

tgacttgaag?aacgagaaag?gcaagttcat?catgagcaaa?ttgagctgaa?ctggaaaatt 18540

agatgtctgg?gctttggagg?ctctgggaaa?aatgctgatt?accaacattc?ctgaaatact 18600

gtctgctata?gaaaatgact?gtctcttttc?tctgtttaaa?aatttagata?cctatatgaa 18660

attgccagaa?gacatcccta?tttctacgcc?ccagaactcc?tttattatgc?cattatatat 18720

aaagatgttt?tttcagaatg?ctgccaagct?gctgataaag?ctgcctgcct?gttaccaaag 18780

gtattccccg?aagaaaagca?gaaccaactc?ataatcaaac?ctcagctttg?gggcatttta 18840

tggctggatt?tagggaacct?aagcctctga?aagaaatttc?tttacctggc?aacagaagaa 18900

agggtggggg?aaaaaatgta?attgtaatgt?atacatgtaa?ggataacctg?acccccttgc 18960

agtacagtgg?gaaaataaaa?aaaaattatt?aaaaaaaaaa?ggttaaatat?ggcaaaaaaa 19020

aaaaaatcca?acctaaataa?aataactaaa?atcagaaaaa?aaaaaaaaag?aaagctttcc 19080

ttcctcttct?ttccctcaaa?gatctttcac?cttttttgaa?gacctatgtc?ttctatcttc 19140

ctgcttctaa?ggtttgccga?agtgggtctg?gtagagggaa?tcactatgta?tgtgtctaaa 19200

tatcccgcct?ctttttatag?tcctagttgc?tatagtgaaa?tacggaaaat?catttttatt 19260

ttattttaat?taattaatta?attaattttt?tgtctttttg?ccttttctag?ggccgctcct 19320

gcggcatatg?gaggtttcca?ggctaggggt?caaatcggag?ctgtagccgt?cggcctacaa 19380

cagagccaca?gcaacgcggg?atccaagctg?cgtctgcagt?ctaccccaca?gctcacggca 19440

aggccggatc?cttaatcctc?tgagcaaggc?cagggatcga?actggcaacc?tcacggttcc 19500

tagtcggatt?cgttaaccgc?tgaaccatga?caggaactcc?cagagactca?tttttaaaat 19560

Pig serum albumin gene group gene order

tgcatagcaa?gagcactcgg?tttctgctgc?atgagacttg?aaacatgaaa?aaaaatttca 19620

tgggactttt?tttaattgga?gaaggaattc?taaatggggg?agatgttaac?tacatctgga 19680

agcctgagtt?tatttattca?ttccataaat?atttattata?tactgcattc?tcttttctag 19740

ggggtttatt?gatgttaaga?gagttggggg?gggaggagaa?attgacatgg?gttatatgca 19800

aaattaagag?gtcagcaaag?ttctgttctt?aggatattag?tgtattctgc?ccttacactt 19860

ttccttttag?ttcccttttc?cctagaaatg?cctccatgca?agtttttaac?catgtagagt 19920

tttccactat?gaccacactg?aattttcctc?tttctgcata?gcaagataat?tatttgccag 19980

tagtgagtac?ttaggaattg?aggtgtaaat?tatctcttta?aaatttaatt?tggcttagcc 20040

tcatgctgag?cttatgaagg?ggatggtgct?gtgcagaatt?tttgctttcc?taatttttcc 20100

aaaattattt?gctttgcctg?cagattgagc?atctgagaga?aaaagtactg?acttccgccg 20160

ccaaacagag?acttaagtgt?gccagtatcc?aaaaattcgg?agagagagct?ttcaaagcat 20220

ggtatttaaa?cttagtttac?atctctataa?tgatgtaaat?catgatggtt?cagctgacaa 20280

attgtacatt?tatatgccat?tttacaaagt?actttcacct?acatcttttt?ggatgtctaa 20340

aaagtagaaa?tttgacagcg?gtgaaaacca?gagcatcaat?gacaatttgt?catttttttt 20400

atcgtatttt?ttctgagtaa?tcaaagaatt?tagaggacag?ctttttgtgg?ttgttcttct 20460

aggacaagtg?tttaaactct?catagattta?taatgattga?gatctcagta?ttaaatagta 20520

cggcccatat?ttttatagaa?atgtagttag?ggtctctaat?cgtacttcac?ctccattctc 20580

acaaccaatt?atctgaaggt?gtcttcttgt?cttcttacgt?ccttgtactt?aatttctgcc 20640

atcaagtttt?cttctcattt?tctcagagct?ccattcattt?tcttaatctc?tttcttttgc 20700

ctgggcctca?gtttgttatt?agctagttct?agaagcatcc?ttcaatacct?gagccaagga 20760

actggctcct?ctcctcatca?atctttcact?ttctggacaa?caatgaagca?acacaaacag 20820

gtaaacaaaa?taaaaatgac?gtttttcaag?ctctctagaa?actgtcactt?gagctaacta 20880

tctttgccca?tcaaaccacc?aaaatcaacc?aaatcagtaa?atgaaacaac?cctaacatga 20940

aatgatacac?aattaaatag?actatgatta?gtttggggag?aagaagtgtc?ttttattaga 21000

tttagtgagt?ctgaaatgat?ctgggaaaat?aggtggatat?aagtctttta?tatcttgcca 21060

ttgagcttag?agattgctga?atcaatccga?tcggtaaaag?tatgctgaat?catatctctc 21120

taatcttgaa?gaagttatga?aatttctttg?ctcagagacc?tttagaagtc?aggaaaaatg 21180

tgttaataga?aaaagataac?tggaatttgc?atggcacgtc?ccagttcata?cagtgctttc 21240

Pig serum albumin gene group gene order

attagtgtta?tataatgtaa?tcctcccaac?agtcctttgg?tttagcagct?gttgtgattc 21300

cgtagagatg?gaagctcaga?gaggctaact?aacttgccta?gggtcacact?gttaataagc 21360

tgtaaagaca?gatctggaag?tcagctctgg?gctccaaact?tgatgcctct?cagaatgttg 21420

ccttttttat?aatccctctt?tgatttctgc?tttatgaatt?gctggacttc?tagccaccat 21480

acacactctt?aaaatgaatc?attctgccct?aaggaaacat?tttgccctta?gataacagtt 21540

tgattaagaa?ctagtattaa?aataagaact?tatgaatttt?atttttttat?ttaaaaaaat 21600

ttttaatttt?aactgagaat?tgcaatgatt?ttttaatatt?tactttttat?taatgtttta 21660

tttatttatt?tattttgctt?tttagggccg?catccactgt?gtatggaggt?tcccaggcta 21720

ggagtcaaat?cggagctata?gctgccagtg?tacaccacag?ctacagcaac?gcaggatctg 21780

agctgtgtct?gcaacctaca?ccacagctca?ctgcaatgcc?agatccttaa?cctactaatt 21840

gagaccaggg?atctaactcg?aaacctcatg?gttcctggtt?ggatttgttt?ccgccgagcc 21900

acgatgggaa?ctccgagaac?taattagttt?tacaaatgat?ttctttatgt?tattttgtcc 21960

taaattgtct?tgttatatgt?agtattttcc?tagcacgttc?acataaaatg?ttgcctgcta 22020

ataatatttt?gatgggaaaa?atttattttt?aggtcattag?ctcgcctgag?ccagagattt 22080

cccaaggctg?actttacaga?gatttccaag?atagtgacag?atcttgcaaa?agtccacaag 22140

gaatgctgcc?atggtgacct?gcttgaatgt?gcagatgaca?gggtaaagaa?tcctcagtat 22200

gctttttggt?aacttgtgtg?gattagaatc?acttggttta?gcatcaaaac?aactgagaat 22260

ggattaagat?tgttttcaat?gctctaatct?gctcaatcct?ttctctgaca?agtggtactt 22320

tattttatct?gataaatgac?tcagtaagtg?ctctaataaa?ctcattcact?aatttgtaat 22380

ccctttcatt?cataatctga?gaacaaaagc?ctcaaagtga?agtaagacag?ttacaagggt 22440

agactttttt?tttggctttc?tttttttttt?tattactcaa?tgattttatt?acatttgtag 22500

ttgtacaatg?atcattacaa?tccaatttta?tgggatttcc?gtcccacaac?cccagtgcat 22560

cccccacccc?caaaactgtc?tcctttggag?accttaagtt?tttcagtctg?tgagtcagta 22620

tctgttctgc?aaagaagttc?attctgtcta?caaaggtaga?cttctaagaa?gttttttttt 22680

tttttttttt?ttttgtgagg?tgggaggagt?tggagaaggg?ctgggatctg?aagagacctt 22740

tggaaattac?ataccttttt?tcgctccata?ttctctctta?attaactctg?ctgtgttaaa 22800

tacaccaata?tttagtatgc?tatgctcttt?ttctcaaact?gaaaagtata?actgaatacc 22860

aaaatcctaa?tctcctcagg?acactttctg?gaaaatttaa?ggcagacatt?tcataagttt 22920

Pig serum albumin gene group gene order

tagtaaggtc?tgtgaccatg?atggtgacta?actctccaaa?aatctcctac?acctgattta 22980

gctttttaag?ttttgagatg?cctgaattta?atctgggggg?aatatttgga?aaaagagtac 23040

tgatttaaac?acttaaatga?tttaacagtg?ttaaataaaa?ttaataaata?attgatagga 23100

gtccctataa?ttgactagat?ttttttttaa?tcaagtgcaa?atgttttagt?aattctgagt 23160

ctctaggctt?agcagcacat?acaaactgtt?cacagaagtt?ggtatatgta?tagtcttaga 23220

acacgctctg?ccaacagagt?caagtctgag?aaggaagtac?agtggtgtca?atttatgtgg 23280

aacaatttcc?atcaacttgc?ttgtaggcgg?atcttgccaa?atatatatgt?gaaaatcaag 23340

acacaatctc?cactaaactg?aaggaatgct?gtgataagcc?tctgttggaa?aaatcccact 23400

gcattgctga?ggcaaaaaga?gatgaattgc?ctgcagacct?gaacccatta?gaacatgatt 23460

ttgttgaaga?taaggaagtt?tgtaaaaact?ataaagaagc?aaagcatgtc?ttcctgggca 23520

cgtaagtgga?taaggattaa?tcctttcata?attttataag?gtctcaggat?ttaggaaatt 23580

attcaggctt?tcttttggaa?ttgccacaat?tttcatgttg?cctttaatgt?ttctttcttc 23640

tttcctcttc?cagcctttaa?acttttaggg?aaaaaattaa?tttaaaaaat?tgtcataaag 23700

taatacatac?tcgtgggaaa?agctcaaaca?atagaatacg?aagcaaaaat?aaaagtcagt 23760

ttttattccc?cccaggaata?cttttggaaa?tactggtaga?aatattttgt?gtatatataa 23820

atttatacat?attttttact?catgcattca?ttcaaagaga?attttttgaa?cttatatata 23880

tgtgaatata?tgtaatatat?ctttatatct?atttatctat?ctacctaatg?gcagcaaaga 23940

actataaaga?aaaatatggc?atctgtttat?atttggttaa?aaagatcaaa?agtccagtaa 24000

catggcaagg?cagcatacaa?ttaaatgaca?aaatcagtaa?ttatacaaag?tcagtaagtg 24060

tggtgataaa?ttttgcattt?ttattgctca?attataatag?tattctgtgc?actaaataat 24120

atttcataaa?attatgtacc?tgtcaagaat?ttactcagaa?cttttcggag?gtgtaatcca 24180

aatcgaacaa?aatatacctt?atagtaatag?gtgtcattca?ttaaacattc?agtatgtgcc 24240

aggcagtgtg?tgttttgcag?agattacata?tttttcacaa?ttcaccttat?aagatagata 24300

ctattattat?ccccattcca?cagagtagga?aacagaggct?ctgatggttt?taataagctg 24360

ccaaagatgg?tataataaaa?taaatggcag?agccaggatt?tgacagattt?atctctgaac 24420

cttcaagtcc?ttagcaatta?agctttactg?catcgggctt?agdaattaag?acctggagtt 24480

cttttcgtgg?ctcagcagta?acaaatccaa?ctaggatcca?tgaggatttg?ggttccattc 24540

ctggccttga?tcagtgggtt?aaggatttgg?tgttgccatg?aggttgcaga?catagcttgg 24600

Pig serum albumin gene group gene order

gtctggcgtt?tctgtggctg?tgacgtaggc?ctgcagctac?agctctaatt?tgacccctag 24660

cctgggaacc?tccatatgct?gcagtgcagc?cctggaaaga?ccccccaccc?caccaaaaaa 24720

aaaaaaaaaa?aaaaaaaaag?agagagaaat?tcagaccttt?aagacaggag?ttacttttgt 24780

tagttaagtg?atctttcttg?ctctttggtc?caaccgaatc?tattttattt?tcatcttaat 24840

taggtttttg?tatgagtatt?caagaaggca?cccagactac?tctgtctcat?tgctgctgag 24900

aattgccaag?atatatgaag?ccacactgga?ggactgctgt?gccaaagagg?atcctccggc 24960

atgctatgcc?acagtggtag?gtttctggag?gataaaaact?gtagctcttt?gactgctgat 25020

tccagtaaca?gaaaagaaaa?ataataaaag?aatgctatta?gtgaaactta?gatcttaaga 25080

tggtttcaat?tctgtgcttt?taaacattat?agaaataata?gccttaaata?gaaaagaaag 25140

atcaaagaga?gccatctcta?aatttacaac?ccctgactat?gctgcttctt?tttttacact 25200

cctgagaaca?atgttaggta?aagtaaattt?aaatgttaat?taaaagacat?ttaacttaga 25260

tataaagaaa?gttaacttga?ttccaagttt?tatcaagtgt?caaaaattag?tatctaggag 25320

ttcctgtcat?ggctcagtgg?ttaacaaatc?caactaggaa?ccatgaggct?tcgggtttga 25380

tccctggcct?tgctcagtgg?gttaaggatc?cggcattgcc?atgagctgtg?gggtagctcc 25440

cagacacggc?ttggatccca?tgttgctgtg?gctgtggtgt?aagccagcgg?ctacagctcc 25500

gatttgaccc?ctagcctggg?aatctccata?tgccacgggt?gcagccctag?aaaagcccaa 25560

aaaaaaaaaa?aaaaaaaaaa?aaaagaatta?gtatcttaac?aaagattgtg?aaattctatt 25620

agtttagaat?attgtcaaat?ctttttttga?gatggtttgg?gaaaggtttt?ataagaaatt 25680

aaagagaaga?aatctgtttc?aagaatttct?agcatattta?gctatgctaa?aatagaattt 25740

tattttattt?gagatattta?acactcctag?cacatatagt?ggtttacaaa?cttaagaatc 25800

caaggaggag?ttcctgccat?ggctcagtgc?ttaatgaacc?tggctagtat?ccacgaggac 25860

atggattcaa?tccctggcct?cgctcagtgg?attaaggatc?tggcattgct?gtgagctgta 25920

gtgtaggttg?cagatgcagc?tgggatcctg?ccttgctgtg?actgaggtgt?aggcaagcaa 25980

ccacacctcc?aatttgaccc?ttagcctggg?aacctccata?tgccgagagt?gcgacctcca 26040

aaaagcaaaa?accaaaaagg?aaaaaaaaaa?aaaaagaatc?agagaaaagt?cattttttcc 26100

tgtaccgaga?acctgttata?tgccagatga?cacacaggga?cttatgtctc?taatctcaga 26160

gagctcagaa?ttcacctgga?gagatggctt?aataatagtg?atacttagaa?tcatagccat 26220

ctttaagttt?tttctatact?ccagtctctg?ctttaatttc?attagctcag?gtggtttaca 26280

Pig serum albumin gene group gene order

gagtaatttt?gtgaagtgag?ttcatctcca?tcgtagaaag?gagggttcta?aagcttagag 26340

atggggagca?atgttgaaca?actggaaaat?gagtcagatg?gcctggcttc?cacaccagca 26400

ctctcactcc?atagtctgaa?tgttcttaac?cgcgtatcct?atacagtctc?accaaataaa 26460

cagtttggac?atgttataac?agatgtggtg?tcaagtatct?atttacatgg?ctttgggcag 26520

aaaagaggaa?ggatctcatc?ctacccagaa?gggaccaaaa?caacctgcgt?ctggtcctca 26580

gagtgtcata?aacacaatcc?ttttattttt?cagtttgata?aatttcagcc?tcttgtggat 26640

gagcctaaga?atttaatcaa?acaaaactgt?gaactttttg?aaaaacttgg?agagtatgga 26700

ttccaaaatg?cgtaagctat?ttttgcttat?tggctaattt?ttttcttgat?tacttagtaa 26760

gtatttaaga?caatatatag?actacccata?atagcacttt?taatcatgac?atgaagtata 26820

cattttgaat?cactctccat?caagaaagcc?cagagaaaat?gagataaaat?agtcgacagg 26880

ataaagaggt?ccatatttca?gtgtagttta?acaaagtggc?ctcgtcagat?tggaggaaaa 26940

atatcaaatc?ttggaatata?gggactgagg?atgccccttg?aaggttaatg?aatattttat 27000

gagtgaacaa?aatacagcaa?tgctttatgg?agcaggaaat?agtcagtctt?gaatggcagt 27060

agctcagtaa?tatgatagat?taatatcctg?atttaatagt?actttattta?tggaggtgac 27120

aagaaagtac?acatcattca?gtaaatagtc?attgttcagc?ccgcgtatgg?tagcactctt 27180

caaaattggt?aagaacagca?aaaacaacaa?tgaagacaca?gtctcttccc?ttgagtagac 27240

tacagtcata?tattggcttt?cagtggtaat?tagcattctt?agtaaatgat?tattacatgc 27300

tagtcaccat?gtcaggctga?ttgtatactt?cttttctttt?aatttaatcc?ttgcattatc 27360

cctattgtgt?cagatatatt?cccttccatt?ttataggtga?gaaaattcac?tcatgtcttt 27420

taaatttagt?tcagtactga?tatatattaa?gctctcagtg?gattttattg?aaacaaagaa 27480

gcagctaata?agtattttaa?tgtcactgaa?ttataataag?ttaggtcact?ctcctaaact 27540

tatacaggat?aagtagaatt?caaagctagg?cttaattagc?tgctcataac?aggccttccc 27600

tcacattacc?ggaaactgga?gatgaagata?attttatgaa?tggaaaatca?tcttagtgga 27660

ttttggctaa?gtgttctctg?cttgatagac?tgttagaaaa?agacagtttc?ttgcctgcct 27720

gaaaacacat?gggttatttt?gttacaggct?catagttcgt?tacaccaaga?aagtacccca 27780

agtgtcaact?ccaactcttg?tggaggtcgc?aagaaaacta?ggactagtgg?gctctaggtg 27840

ttgtaagcgt?cctgaagaag?aaagactgtc?ctgtgctgaa?gactatgtga?gtcattggag 27900

ttcccgtcgt?ggcttagtgg?ctagcaaatc?cgactaggaa?ccatgaggtt?tcgggtttga 27960

Pig serum albumin gene group gene order

tccttggcct?tgctcagtgg?gttgaggatc?cggccttgct?gtgagctgtg?gcctgggttg 28020

cagacatggc?tcagatcctg?agttgctgtg?gctctggcac?aggctggggg?ctacagctcc 28080

atttcgaccc?ctagcctggg?aacctgcata?tgccgcagga?gcagcccagg?aaatggcaaa 28140

aagacaaaaa?aaaaaaaaaa?aaagattatg?tgattcattt?aaaatatact?gtggttacag 28200

agtttccatc?atgcgcagag?gaaatgaatc?tgactaggaa?ccatgaggtt?gtgggttcga 28260

tccctggcct?cagtcagtgg?gttaaggatc?tggtgttgcc?atgagccatg?gtgtaggtca 28320

aagatgtggc?ttggatcccg?cattgctgtg?gctgtggtgt?aggttggtgg?ctgcagctcc 28380

gatttgaccc?ctagcctggg?aacctccata?tgttgagggt?gtggccctaa?aaagacagaa 28440

agacaaaaaa?atatatatta?tggttgataa?tgataacttt?tttcctgtag?cgattcacaa 28500

agcgaacttt?gagaagcaga?ggtggactaa?cctgggtgtg?tgtcgtgtat?atgatggtga 28560

cagccaagac?ttggtcacct?gtagtattct?gaccatcaga?atgaggagtt?agataagatt 28620

ccaaaagtga?aacattgttt?tgaaaaaaac?gccatgaatt?ttcaatgttc?tatcctggca 28680

atttctaaat?tttaactttc?tttgggtctt?ttctttaatg?tatcagtata?agaatttttc 28740

cccctattcc?aactcgtatc?tcagggctca?cctcaaatta?tttactcaat?taatttctca 28800

aataaattat?tcttaaaatg?tagacatata?taattaaaag?ggttttcaaa?gtcccttata 28860

ttcgttttta?tgataagcaa?ctcactgcct?cccctcaacc?ttgggttcag?tgttgctaat 28920

taaaatagaa?catttgggag?ttcccgtcgt?ggcgcagtgg?ttaacgaatc?cgactaggaa 28980

ccatgaggtt?gcgggttcgg?tccctgccct?tgctcagtgg?attaacgatc?tggcgttgcc 29040

gtgagctgtg?gtgtaggttg?cagacgtggc?tcggatcccg?cgttgctgtg?gctctggtgt 29100

aggccggtgg?ctacagctcc?gattcaaccc?ctagcttggg?aatctccata?tgccgcggga 29160

gcggcccaag?aaacagcaac?accaccaaaa?gccaaaaagc?caaaaaaaaa?aaaaaaaaaa 29220

aaaaaaaaaa?aagaccaaaa?aaaaaaaaaa?aatagaacat?ttgttctggt?ccactggaaa 29280

gtaggaattc?atggaaaact?tttcattatg?cttcttttga?atttctgcgc?tcctgcctgt 29340

tttcagctgt?ccctggtcct?gaaccggttg?tgcgtgttgc?acgagaagac?accagtgagc 29400

gaaaaagtta?ccaaatgctg?cacagagtcc?ttggtgaaca?gacggccttg?cttttctgct 29460

ctgacaccag?acgaaacata?caaacccaaa?gaatttgttg?agggaacctt?caccttccat 29520

gcagacctat?gcacacttcc?tgaggatgag?aaacaaatca?agaagcaaac?gtgaggagta 29580

tttcattact?gcacgcattt?gtggtcacga?cagtgagaac?tgtaaattga?aaagaacaac 29640

Pig serum albumin gene group gene order

ttctgaagta?gtgattatcc?accttaggag?agagtactta?agtattggcc?ttactaaggg 29700

gattaaattg?tctagaatta?aatggatggc?attttgttct?cttagcaaaa?gaaaaaaaat 29760

tgttatcata?atattgctta?caaatcattc?agaataatcc?tgtggataaa?ggctgcagta 29820

gaataatatt?ttaggctagg?cccagtagaa?aaagctgaac?gattaactga?tacatttttg 29880

cagatagcaa?actagtagaa?atcatagggg?aatttatttc?taggcatgga?tggctatttt 29940

cagtttttct?atggcaacct?acaggcaagg?caaattttca?agatctgact?tttgaaaacg 30000

aaacttggat?aaaaaaacat?ttagggctgt?gagttctgag?gcctattgct?gtcataaggg 30060

gtcacaaatg?catgaaattt?ggcaacgtaa?agttgctatt?atctctagtg?gcttctagtc 30120

atggccatag?acttaagagt?aatattgcaa?ccctatcata?gcccacatga?ccctttattg 30180

agcaccactg?tctctgcaca?tgtcaatgga?agaggaccac?tagctcccac?agatttagac 30240

agtcttattt?cctatggcct?ctccgctatc?aattttgaat?gggcaagttc?aaaagccaaa 30300

tataaagatt?ctcaggttag?aaactttcta?aatggtgccc?aagataaata?agtttttaaa 30360

gatcattttc?agttcttaaa?tagctataaa?attcaatgta?gcataactcc?tcaaatctga 30420

ataatgtgta?attgcaatga?catcatcttt?tccagtgatt?tactagagaa?caatttgttg 30480

ggattttctg?acaccacaaa?tgttcagcaa?tttacattga?ttacttaatt?tgatccttcc 30540

accaaccctt?tgaaggtgga?ttttattctt?ctcatgatgc?aaatgagaat?atttaagctt 30600

gtaatggtaa?gcctgggcct?caaacccaga?gttgcctagc?tctaagactt?ataatcttaa 30660

gcagcgctgc?tatcatttta?ctatctctgt?ttttctttag?ggagtaaatt?ttttttctgt 30720

gtttttgttt?gttttttgtt?ttcattccaa?tgtagtgcac?tcgttgagtt?gttgaaacac 30780

aagcctcatg?caacagagga?acaactgaga?actgtcctgg?gcaactttgc?agcctttgta 30840

caaaagtgct?gcgccgctcc?tgaccatgag?gcctgctttg?ctgtggaggt?actggatttg 30900

tttgtcttca?ttctaatggg?tccagtttcc?tttgttgttg?catttgatca?agctaggact 30960

tagtggctta?tgtatctgaa?aaacactatg?tacatgcgga?atgagaatct?tactcttcat 31020

tgtcttacaa?aatgactgag?atagctcttg?cttttgtttg?ttttaatatc?ctgcttcact 31080

gtaacttatt?actaaatgtc?tgtaataatt?gttttgtttc?caaagttgtg?atgtttataa 31140

atatagatag?aaatatttgt?aaggcccaaa?atcttgttat?gaaaccaaaa?cattaattta 31200

tttaaacatt?ctcttgaaaa?atgctggttg?gtgatctgga?tgaatcggaa?tgcttgattt 31260

tacaggtgac?tggggaagtt?tatatttaaa?tggctgaatc?atttaacgtt?tatccctatg 31320

Pig serum albumin gene group gene order

atttgacagt?aattttttta?ttcctttggg?ggcagtgata?ccagtgaact?tccttctaga 31380

gagtctagta?ctaaacagga?attagaggat?gtgatgtatg?taaaattact?tcgtaatcac 31440

caatgactta?gtaaatgtta?cttttttaaa?atcctaataa?tggtgctaat?attttctcca 31500

tacctgtcat?ttctttgtat?ttagggtccg?aaatttgtta?ttgaaattcg?agggatctta 31560

gcctaaacaa?cacagtgaca?agcatctcag?gtaactatac?tttatatttt?ttttttctta 31620

aaagtagctg?tggggagttc?ctgtcgtggc?gcagtggtta?acgaacccga?ctaggaacta 31680

tgaggttgcg?ggttcggtcc?ctgcccttgc?tcagtgggtt?aacgatccgg?cattgccgtg 31740

agctgtggtg?taggttgcag?acgcggctcg?gatccagcgt?tgctgtggct?ctggcgtagg 31800

ccggtggctg?cagctccgat?tagaccccta?gcctgggaac?ctccatatgc?cgtgggagcg 31860

gcccaagaaa?tagcaaaaag?acaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaagta 31920

gctgtggaat?tccctggtgg?ctcagcgagt?taagggtcca?gcatttttgc?tgctgtggct 31980

ctgattactg?ttgtggggca?ggtttgatcc?ctggcctagg?agcttctgca?gaccgcaggc 32040

acagccaaag?gggaaaaaaa?agtaattata?atacttatta?ttgaaagcaa?agagtgactg 32100

accagtgcca?gtcactattc?taaactcatt?atatatatta?acttctttaa?ttctcaaaat 32160

atttgcctga?gttgaatacc?attattattc?tccttttgca?aatgagaaga?cttctcctgt 32220

ggctcacatc?ataaccattt?ctcaaatgag?gaaaagaagg?cacagaacaa?ggcctaggct 32280

aaataattta?cctaaggacc?actcaactag?tgagaggtgg?ggccaagatg?caaaattcaa 32340

accagggctt?gagagctcac?cactgtcctt?tgtgacctaa?tgtggtgcag?aagagtcatt 32400

taactaagca?ctggctcaac?agtgggttac?tttaggtaga?ggctagacta?aatgcaagtg 32460

catttctaca?tgtaaaaacc?caactgaata?tcactgattt?cacatggtta?accagatagc 32520

tcaagaacaa?attcatttca?ctggaggatg?tgatagttca?acctgtgtga?acttctactc 32580

taagtaaata?atttgctaca?gtttccaatg?tgttgacatg?ctgggttttt?tgtttgtttg 32640

tttgtttttc?ggtctttttg?ccatttctaa?ggccgctcct?gtggcatatg?gaggtgccta 32700

ggctagggat?ccaattggag?ctgcagctgc?tggcctacac?cacagccaca?gcaatgcggg 32760

atccgagctg?tgtctgcaac?ctacaccaca?gctcacggca?acgccggatc?ctttaaccca 32820

ctgagcaagg?gcagggaccg?aacccacaac?ctcatggttc?ctagttggat?ttgttaacca 32880

ctgcgccact?acgggaaccc?cgagaacttc?ttttcttatg?acaaaagatc?ggctttaaaa 32940

tttagatgtt?tgaacataaa?catgttttac?ccattttggt?tataatattt?tttgagaagt 33000

Pig serum albumin gene group gene order

taaaaaaata?agatgaactt?ccttttttga?aactagttgt?ccagctatat?tttattttat 33060

tatgttttgg?ccacctcaca?gcatatggag?ctcccgggcc?agggatgaga?tctgagctac 33120

agtcattcgc?aacctaagcc?atagctgtgg?aaatgctgga?tttttcaccc?actcagacag 33180

gctggggatt?gaacctgcat?cccggccttc?ccaagacact?gccaatccct?tcatgccaca 33240

gtaggaactc?catccaacta?ttttttagca?tgaataattt?tcatattttt?caaactgatt 33300

gtgatgttta?ttccactgtg?aggttttaac?cttgctcttt?gctaatagga?taatacttat 33360

aattgtgcat?tcggccaaag?aaccaacttc?agatctctgc?aaatttgggc?atttcaaaat 33420

gtgatttgtc?ttagtcctaa?atggcaagac?tttcttccta?gaggtctaac?ttggagaaga 33480

cacctttttt?gtttgtaatt?aacttagtga?gaagtagatg?ttaagttaat?gactctgagg 33540

gtcgatgagg?aaaaggctga?ggagtccaat?gacactggtg?tcaaagagag?gactttacaa 33600

tccttatagc?ccaacagaag?aatttagccg?ccatgagtct?aggacagcct?taaattgttt 33660

tcactggtgg?aaattacaga?aagacagtct?aagcaattta?gcattcattt?gaatagattt 33720

gagaaaacac?acagcatttt?ataaacagta?attatattta?tccttttgtt?tttcagacta 33780

ccctgagaat?aagagaaaga?gaaatgaaga?cctagactta?tccatctctt?tttcttttct 33840

gttggtttta?aaccaacacc?ctgtctaaag?tacacaaatt?tctttaaata?ttttgcctct 33900

tttctctgtg?ctacaattaa?taaaaaaatg?aaaagaatct?aatttaattg?tctatgactg 33960

ttattttttt?gaagatgtgt?tgtcaacctg?ataattttgt?aggttctatg?aaaattccac 34020

tattctctct?ttccctattt?caatggagga?cttctagttc?cttctggatt?aattgcataa 34080

aagaaacatt?aatactgcca?tgagttatgg?attatgaaca?ttcaaagtaa?tattttaaca 34140

ttatgataat?tctgaataaa?agagtagagc?ctgtggtata?ggtgagacat?ataaaacatg 34200

gtttatggag?ttcccttcat?ggcacagtgg?aaacgaatct?gactaggaac?catggaggtt 34260

tcgggttcaa?tccctggcct?cactcagtgg?gttaaggatc?cagtgttgct?gtgagctgtg 34320

gtatagtttg?caaacttggc?tcgtatctgg?cattgctgtg?gctttggcct?aggccggcag 34380

ctgtagctcc?gatttgaccc?ttggcctggg?aaccatcata?tgcagcagaa?gaggccctaa 34440

aaagacaaaa?agacgaaaaa?ataaaaaata?aaaataaaac?atggcttaat?ctcactacta 34500

gaacttagtg?aataaatgtg?tggcaacaag?aaggcagagg?tgattgtaag?gatacagttt 34560

tccccccttc?tccacaggag?atgcgtttca?agactcccag?tggatgcctg?aaaccacaga 34620

tgtggcaaat?cttatataca?ccatgttttt?ccctgtacat?acatacctat?gataaacttt 34680

Pig serum albumin gene group gene order

catttataaa?ttaggcagag?taagagattc?acaataacta?ctaataaaac?agaacaattg 34740

ttacaatata?ctgtactaaa?agttatgtta?atgtggtctc?actctctcaa?aataccttat 34800

tgtacaattt?taatgccttt?tccatctaaa?ctaagcacat?atcacacaca?ctgcggccat 34860

atgacagcaa?aactagcatg?gatttctttt?tccttcttca?gaatttcaca?gataaagaga 34920

tataccctca?ctgtagatca?acctcagcat?acagttcttt?tttttttttt?tggtcttttg 34980

tcttttcagg?gcagcatcca?tggcatatgg?aggttcccaa?gctaggggtc?caatcggagc 35040

tgtagccacc?ggcctacagt?agagacagca?atgccagatc?taagctgcat?ctgtgaccta 35100

caccgtagct?catggcaaca?ccagatcccc?cacccactga?gagaggacag?ggattgaacc 35160

tgcaatgtca?tggttgctag?ctgggtttgt?taaccactga?gccacgacag?gaactccctc 35220

agcatacagt?tctttttctt?tccttactaa?atggaaaatt?tttgcttttt?cacttgaccg 35280

aagcacttta?cttttctctc?tggcagatcc?gaattg 35316

The target practice sequence of pPHA1

SEQUENCE?LISTING

＜110〉Biologic Engineering Inst., Academy of Millitary Medical Sciences of P.L.A

＜120〉be used to produce the pig serum albumin gene seat targeting vector construction process of foreign protein

＜130〉the target practice sequence of pPHA1 carrier

<141>2004-06-06

<160>1

<170>PatentIn?vetsion?3.2

<210>1

<211>13971

<212>DNA

<213>human?and?porcine

<400>1

atcgatataa?ctcaagatct?caccatctgt?tgctaggaat?attgcattag?tgccctgact 60

agtcttccta?gacatactct?tgccttctct?agtctcttcc?ccacattgca?accagagtag 120

tcttctaaag?tatagatcgg?attatattat?tgggctaata?atatcattca?acttctcaat 180

gcttttagaa?tgaagaccaa?aaatactgct?atagctaaga?tgacttccaa?atccatcttc 240

tctcatctca?cactgtgctt?ctcctcaact?ctgtgtctag?ctggtctcag?tttctttcat 300

tctctttctt?ttctcaagca?ttagcacatt?ctttcccctg?tgcttgtaag?gctattttct 360

ttcatctttc?tttatgccca?ttttaaaaag?tctcagttta?gaagtcatgt?tcctaggaaa 420

gccttctcag?acagacttcc?aggcacatgg?tattattaaa?taaatatggc?ttgaataaaa 480

aacaagtatt?tatcactaaa?tacttgggtg?gcctactgac?tttgtcagtc?ctctcaagtg 540

aactatttcc?ctataaaagt?tttgtagctt?tctgcaagaa?tatgtttctt?tgggatttat 600

aatattaaga?tgacctatct?catccttaac?tttgcctgca?aatccaaata?attgcaagtt 660

ctgcttttct?attgctgact?gccagcctgc?ctttcttttc?tttcttactc?ctgcaggaaa 720

tttcattgtc?ttctaaactg?aagtctaact?ctttgcttgc?tgcattttga?tcttttctca 780

aacatttaga?aattacacat?attcataccc?tccttacttc?cctcaatcct?ctacctactc 840

attccatttc?ttcaggtgac?aattccttca?caagcaaaaa?tgaacccaga?gcatgcaact 900

ttcaagcttt?tgtctgttaa?gttgcatagt?tagctggagt?ttagagcccg?tatatcttat 960

ttgtgtgtga?gattgtcctt?ggatttagag?cttctcctct?gatacttttt?tatcacccca 1020

ggagtccttt?gtgttctaca?tgcacagtac?tttacaatgt?tgtattgtaa?ctatcttcca 1080

The target practice sequence of pPHA1

gtgtgtctat?cttcccccta?ttaaactgcc?tgcttcatga?cctatggagg?gcttcaagaa 1140

cttaagagtc?agggactgtg?tcttgttttt?attgttttta?acctcacatc?ctaggaaatg 1200

cttggggagt?gagcacccca?tacattttga?gcatgaatga?aggggtaggt?ggctacttaa 1260

aataataagt?ctgaaacatt?gtttcagaac?tgtgcttgtc?agcaagaacg?ctgatgttcc 1320

taagtaaata?gtgcttttgt?tgatgccagg?acaatgtttg?cttttagata?ccatgattct 1380

caatgtgcat?tttttgccaa?ttttccacgt?aataatgaag?agaaatttga?actcatcaat 1440

aacggtcatt?tgcattcaag?gcattaaatg?ctgagaggct?aatttttata?attttaatga 1500

atgaatgaat?atctaatata?aatataaaaa?ttaaaatcga?attgaaataa?taattgtata 1560

tatttcatct?aataacttaa?actatttgta?ttaaataatc?taaatatttc?acaactgtga 1620

atcagaagcg?ttttggaaag?agcatgccat?tttgtcacat?tatatgctgc?tgaaattact 1680

tcttcctgct?gtttgaaagc?atatgttcat?ttcaacaggt?tattaccaaa?tagctatttt 1740

tgcaaatgga?aacttcaagc?ctctaaatat?tattctaata?aaagaatttt?gctcttttaa 1800

atgtctacat?aatgaaaaac?taatagtgaa?caattcattt?cttatatgtg?caataattat 1860

atttaagacc?atttcaaaaa?tatttatatt?gtatacttta?ttacattata?taggctagac 1920

ttggatttct?cttaaaaaaa?gaaaattatg?ccaattattt?gtttgatata?tttcttaaaa 1980

gttacaagtt?tctttggatc?aaataattca?tttcttgata?ttagccccta?catagatgct 2040

tgaaaagctg?atgagaattt?taagacttaa?agtgtaaggc?tcaggtgggg?ggcaggccag 2100

agaagtttta?gttgaagtcc?atcaatggga?ggttagaaaa?catctgataa?atttgaagct 2160

ttagaagaca?tcaaggagaa?aaaaaaatta?cccttttatg?aaaactttct?gaattttatc 2220

aaccaatgtt?tttaatatag?gactgtttta?gctacaaaac?atttgctttt?ttacaagagg 2280

ttaaaaatag?ttccatttgt?aactaatatc?cttttaagta?ataagtccct?tgaattacat 2340

ttggaaagaa?atttagaata?tgaagagact?ttttattata?ttattatatt?aaggacaatt 2400

aaacccccag?taatgtatta?aattctgttt?gatttccatc?tgttgctatt?attatgaaag 2460

agaagttact?agcattaatt?tcattttcta?tgtttgcaga?ctctgttgtt?aacttataca 2520

aagaacagat?aacatggatt?tcaggcatca?gttatatttt?tcttggattg?gaaaaatgat 2580

aaaaagatat?gagtgttatt?ttatttctat?aatttagaga?agtgaaatga?cctgttcatt 2640

tcttaagact?cttaacctga?agtagataaa?gagtgatgcc?taaggaactc?atgggcagct 2700

cagcgatgaa?cagattcgca?gatctggatc?ccaattcctt?aacttgatat?agtcattcta 2760

The target practice sequence of pPHA1

ccagtcaggc?cctttactca?ttctatgact?gtttcattgg?gaatatggaa?tcacagaatg 2820

ttcaaggcag?aagtgtcctt?aaagattatt?taatccagct?tgacccatat?ggcctaaaga 2880

tatagacaga?tctgcacact?tgagtttgca?gctagaaatt?gacattgaga?tgaattaaac 2940

tgcatagtaa?agagttgaga?agttttaagt?aaatctgaga?gaaagtagac?ctaaattcta 3000

gatgcccagt?gtagcatgag?gaaagatgga?aaagggaccc?tcctgccatg?ggcgctttgg 3060

agagatctgg?atttaagaga?tgggacaagc?caaggatggt?aatttagtgt?ggtttttata 3120

atgtaaactt?tcttcccttt?atcccctatg?attttttcag?caaagatttt?tctcactggg 3180

attcctgcca?tggcacattg?gaaatgaatc?cgactaggaa?ccatgagatt?atgggtttga 3240

tccctggcct?cgctcagtgg?gttaaggatc?tggggttgcc?gtgagctatg?gtgtaggttg 3300

cagatgtggc?tcagatctgg?cattgctgtg?gctgtggtgt?aggccggcag?ttgtatctcc 3360

gattagaccc?ctagcctggg?aatcaccacg?tcgcgggtgt?ggtcctaaaa?agacaaaagg 3420

ccaaaaaaaa?aaaaaaaaaa?aaaaaagatt?tttctcacta?atgcaagtgt?ttatggaggg 3480

ctgtgaaggg?acaattgata?tttgaattac?attacaaatt?ttatgtttgc?attagaaatg 3540

cctatattgt?tcacacaatt?tggacaattt?attttctttt?attaatggga?aatgtatctt 3600

cataagtgaa?taagaaatta?aattgcttac?catatttcaa?ttagtttgat?gcctaatact 3660

ctttcacttt?tctatccttc?tgtgttttta?gaaagcagtc?caggctcttc?aatgataaat 3720

aagatctatc?ctacctatat?gtggacatcc?acacatatgt?cccatgcata?ataggaagaa 3780

tgttaagaaa?cgatttttct?gtttaaattt?ccctccaaat?aaatccaaat?ttcccacatt 3840

ttggagtgac?tttgggttaa?atgtttctag?aaagtctgtg?ttgtaatact?gaggctatta 3900

tcagtcattg?ttgtcccatt?ttaatgcata?tgtttccctt?gatttctaac?tgaaggcaac 3960

ccagaagtaa?taatctatta?cagaaggaga?tggcataaag?gttgaagaag?gaaaatggac 4020

aaatggaggg?aaacctggtt?tgaatgactt?ggtggaagct?gcagggatcc?cgaaggtagc 4080

aggagggtat?aggtttaagt?ttttgattag?gatggcaagg?aagtggggga?ctggtccaag 4140

agatacttca?tctgcttcaa?atctgtgcct?tttctttcct?gtcaaaatga?atgcaaaaaa 4200

tgaaggacaa?ggggataaaa?tatttcaact?gaggtatttt?tttctcttct?aaatgatcag 4260

catataatta?actctttcca?taagaagtaa?tcttcttaac?tactctctct?ttaaacttct 4320

cccataaaat?taggaacaca?ttgaaaaatt?attggagcta?gtttcctatt?tatttagaag 4380

tagtttctat?aagtcatatt?taaaactctt?ggtttaaata?ttcacattta?ctcttgtgcc 4440

The target practice sequence of pPHA1

agattcctta?ggtgactaac?taaatacaag?ggtttaaaat?cttgaagaac?agaatactta 4500

gaaataaaca?acaattaagt?aattttattc?atctcagttt?agaatatata?ctcagaatta 4560

atagaagaaa?aatgtaggta?cccttacttt?gggaacctac?aagatgcaaa?tattcaaggg 4620

tttcttaaaa?gttaataatt?ttaaagcaga?tagtgttaag?aattaaatat?tttaatttct 4680

caaccatctg?ctaatcttta?aaaaagatac?atacacagaa?atttcattat?gctattcaca 4740

ggaaacattc?ttttttaaaa?tgagttttat?tttttccatt?atagttggtt?atagttttct 4800

cctgattttc?tactgtacag?caaagtgacc?cagtcacaca?tacatatata?ccttcttttt 4860

ctcacattat?cctccatcat?gctccatcac?aaatgactgg?atatagttcc?cagtgctata 4920

caccaggctc?tcattgctta?ttcattccaa?atgcaatagt?ttgcatctat?taaccccaga 4980

ttcccagtcc?atcccactcc?ctccccctcc?cccttggcaa?ccacaagtct?gttctccaag 5040

tccatgagtt?tcttctctgt?ggaaagtttc?atttgtgtcg?tatattagat?tccagatata 5100

aggaaataac?ctttcaggtg?ttttcagttt?tatatttgaa?aactctgtga?tcttcattaa 5160

atataagaat?atcaatttta?agtttctaaa?attgtaatga?atttgatttg?gggggttttc 5220

tgtatttacc?attttattac?aattgcagtg?caaagtaggt?ggagagacta?atatttttct 5280

tttcatttgt?caaataaaaa?atatgcacga?agtatatgtg?tgagtgacaa?aagcctgatt 5340

ctgtgaatta?gtaatacagt?tatatccaag?gataaggact?gcgtatttaa?taaagccttc 5400

ccagaggact?catgaggaac?actgacttca?aataaaagat?gcctttttct?cagaagctca 5460

aagttgatat?aatttttttt?taaatttaga?aaattagaat?agctgccttt?tttctcttct 5520

agtattggat?tttaggcttt?aaaaaatgat?tttctccaat?ggaaaaaatt?ccatagacag 5580

acattcactt?atttctgttc?agctgtgccc?agaaagacaa?gtctatttct?gttatttatg 5640

gagccattaa?aaaaaaaaaa?tctatcagct?ctgtctagct?aggtagctaa?tcatctatct 5700

gtttgtatta?tctacctctg?taatctatca?aaatattgtc?ttattgcctg?ctttcctatc 5760

atctcctctt?ttgtgcttga?ctatttaatt?agtacccata?cctgagaagt?gaaaaaaaaa 5820

gtcatagaaa?ataaatttct?ttcatcatat?gactgaatag?gaaaaataat?acttctttta 5880

agtatttact?tgtgtctgtg?ccatatgtgc?cacagagtga?catggctgac?agtttgattg 5940

acttggttat?ttaattaact?gaaatgactg?gcttgatact?aattattgtt?ctgtagtatc 6000

aaagtatcag?aggaggttag?ttttcctttt?tcttttgatt?gaaatccgta?gttcttcttg 6060

gctgtgtcga?tgacatgtgg?aagttcctgg?accagggatc?taacgggtgc?tgcagctgca 6120

The target practice sequence of pPHA1

accagtgcca?cagctgtgga?aacgccttaa?cccattgcac?caccagggag?cttcatgaaa 6180

tccatagttc?ttcatcttta?gagcaaaagg?gcagagagag?aatagtcctt?cagaggcaga 6240

gatcagaatt?tggggtggga?aaagtaagga?aggaagttcg?aaagaacatt?tttgaaaaat 6300

tatctttgtt?tttagacatg?aagttaaatt?tatttcgaaa?tttaaaataa?cattgaagaa 6360

gagcatgtga?ctttcctttt?tagtaaaagc?gagaaagaaa?gaaaaatgtg?aaatagggat 6420

ggaaagaata?taatgcctgg?taaagaaggt?ctaggattct?taaaaccact?aatataatca 6480

ggctcagcct?gccctgtgta?tatcatgata?gaaaagatta?ccactgctgc?cattgtgtcc 6540

attttcttcc?ccatatctcc?atgagaggta?ccatactgcc?tttaagtact?ttactctaga 6600

gcctgaatca?ccagtggcca?gagctgaggt?aaaaatgtag?gacttctcaa?catccgtttt 6660

agttgttatt?gttgctatga?ctttttctta?gtttgcattt?gagacactgg?tttttttgac 6720

agaacagttt?cagaagaatg?aaaagccatg?gaagccctcg?aatgcgtgca?tgcatgcatg 6780

catgcatgca?cacacccatg?tgtgtatgca?ttttcactac?ttatcaataa?aagcatactt 6840

ggaattaata?atagatacaa?ttcagagctt?agctctttta?acatgtctta?tttccaagct 6900

aagtcactgg?tttgcttaaa?tttttgcaaa?ttttgaaaac?taacacgttc?aaatggtaaa 6960

ccacagatag?agatcatggt?aatagtacaa?atcgtttaaa?ctcttatgta?aaatttgata 7020

aggtgtttta?cataacttta?atgaattgac?aaggtcttgt?ggagaaaaca?gatccagacg 7080

gcaaacatac?gcaagggatt?tagtcaaaca?catttttggc?aaaaaaacta?tgaattttgt 7140

aatcagttgt?gagccaatga?aatacaaaaa?tgagtctagt?taataatcta?caattattgg 7200

ttaaagaagt?atattagtgc?tgactttcct?ctgttcgtcc?taccttttct?cttctatcaa 7260

ccccacaagc?ctttggcaca?atgaagtggg?taacctttat?ttcccttctt?tttctcttta 7320

gctcggctta?ttccaggggt?gtgtttcgtc?gagatgcaca?caagagtgag?gttgctcatc 7380

ggtttaaaga?tttgggagaa?gaaaatttca?aagccttggt?gttgattgcc?tttgctcagt 7440

atcttcagca?gtgtccattt?gaagatcatg?taaaattagt?gaatgaagta?actgaatttg 7500

caaaaacatg?tgtagctgat?gagtcagctg?aaaattgtga?caaatcactt?catacccttt 7560

ttggagacaa?attatgcaca?gttgcaactc?ttcgtgaaac?ctatggtgaa?atggctgact 7620

gctgtgcaaa?acaagaacct?gagagaaatg?aatgcttctt?gcaacacaaa?gatgacaacc 7680

caaacctccc?ccgattggtg?agaccagagg?ttgatgtgat?gtgcactgct?tttcatgaca 7740

atgaagagac?atttttgaaa?aaatacttat?atgaaattgc?cagaagacat?ccttactttt 7800

The target practice sequence of pPHA1

atgccccgga?actccttttc?tttgctaaaa?ggtataaagc?tgcttttaca?gaatgttgcc 7860

aagctgctga?taaagctgcc?tgcctgttgc?caaagctcga?tgaacttcgg?gatgaaggga 7920

aggcttcgtc?tgccaaacag?agactcaaat?gtgccagtct?ccaaaaattt?ggagaaagag 7980

ctttcaaagc?atgggcagtg?gctcgcctga?gccagagatt?tcccaaagct?gagtttgcag 8040

aagtttccaa?gttagtgaca?gatcttacca?aagtccacac?ggaatgctgc?catggagatc 8100

tgcttgaatg?tgctgatgac?agggcggacc?ttgccaagta?tatctgtgaa?aatcaggatt 8160

cgatctccag?taaactgaag?gaatgctgtg?aaaaacctct?gttggaaaaa?tcccactgca 8220

ttgccgaagt?ggaaaatgat?gagatgcctg?ctgacttgcc?ttcattagct?gctgattttg 8280

ttgaaagtaa?ggatgtttgc?aaaaactatg?ctgaggcaaa?ggatgtcttc?ctgggcatgt 8340

ttttgtatga?atatgcaaga?aggcatcctg?attactctgt?cgtgctgctg?ctgagacttg 8400

ccaagacata?tgaaaccact?ctagagaagt?gctgtgccgc?tgcagatcct?catgaatgct 8460

atgccaaagt?gttcgatgaa?tttaaacctc?ttgtggaaga?gcctcagaat?ttaatcaaac 8520

aaaactgtga?gctttttaag?cagcttggag?agtacaaatt?ccagaatgcg?ctattagttc 8580

gttacaccaa?gaaagtaccc?caagtgtcaa?ctccaactct?tgtagaggtc?tcaagaaacc 8640

taggaaaagt?gggcagcaaa?tgttgtaaac?atcctgaagc?aaaaagaatg?ccctgtgcag 8700

aagactatct?atccgtggtc?ctgaaccagt?tatgtgtgtt?gcatgagaaa?acgccagtaa 8760

gtgacagagt?cacaaaatgc?tgcacagagt?ccttggtgaa?caggcgacca?tgcttttcag 8820

ctctggaagt?cgatgaaaca?tacgttccca?aagagtttaa?tgctgaaaca?ttcaccttcc 8880

atgcagatat?atgcacactt?tctgagaagg?agagacaaat?caagaaacaa?actgcacttg 8940

ttgagcttgt?gaaacacaag?cccaaggcaa?caaaagagca?actgaaagct?gttatggatg 9000

atttcgcagc?ttttgtagag?aagtgctgca?aggctgacga?taaggagacc?tgctttgccg 9060

aggagggtaa?aaaacttgtt?gctgcaagtc?aagctgcctt?aggcttataa?gtcgaccaaa 9120

cccgctgatc?agcctcgact?gtgccttcta?gttgccagcc?atctgttgtt?tgcccctccc 9180

ccgtgccttc?cttgaccctg?gaaggtgcca?ctcccactgt?cctttcctaa?taaaatgagg 9240

aaattgcatc?gcattgtctg?agtaggtgtc?attctattct?ggggggtggg?gtggggcagg 9300

acagcaaggg?ggaggattgg?gaagacaata?gcaggcatgc?tggggatgcg?gtgggctcta 9360

tggatcccgc?gggtaagggt?accataactt?cgtatagcat?acattatacg?aagttatatt 9420

gctagagtca?gcttctgatg?gaattagaac?ttggcaaaac?aatactgaga?atgaagtgta 9480

The target practice sequence of pPHA1

tgtggaacag?aggctgctga?tctcgttctt?caggctatga?aactgacaca?tttggaaacc 9540

acagtactta?gaaccacaaa?gtgggaatca?agagaaaaac?aatgatccca?cgagagatct 9600

atagatctat?agatcatgag?tgggaggaat?gagctggccc?ttaatttggt?tttgcttgtt 9660

taaattatga?tatccaacta?tgaaacatta?tcataaagca?atagtaaaga?gccttcagta 9720

aagagcaggc?atttatctaa?tcccacccca?cccccacccc?cgtagctcca?atccttccat 9780

tcaaaatgta?ggtactctgt?tctcaccctt?cttaacaaag?tatgacagga?aaaacttcca 9840

ttttagtgga?catctttatt?gtttaataga?tcatcaattt?gtgcagactt?acagcggatc 9900

gatcccctca?gaagaactcg?tcaagaaggc?gatagaaggc?gatgcgctgc?gaatcgggag 9960

cggcgatacc?gtaaagcacg?aggaagcggt?cagcccattc?gccgccaagc?tcttcagcaa 10020

tatcacgggt?agccaacgct?atgtcctgat?agcggtccgc?cacacccagc?cggccacagt 10080

cgatgaatcc?agaaaagcgg?ccattttcca?ccatgatatt?cggcaagcag?gcatcgccat 10140

gggtcacgac?gagatcctcg?ccgtcgggca?tgcgcgcctt?gagcctggcg?aacagttcgg 10200

ctggcgcgag?cccctgatgc?tcttcgtcca?gatcatcctg?atcgacaaga?ccggcttcca 10260

tccgagtacg?tgctcgctcg?atgcgatgtt?tcgcttggtg?gtcgaatggg?caggtagccg 10320

gatcaagcgt?atgcagccgc?cgcattgcat?cagccatgat?ggatactttc?tcggcaggag 10380

caaggtgaga?tgacaggaga?tcctgccccg?gcacttcgcc?caatagcagc?cagtcccttc 10440

ccgcttcagt?gacaacgtcg?agcacagctg?cgcaaggaac?gcccgtcgtg?gccagccacg 10500

atagccgcgc?tgcctcgtcc?tgcagttcat?tcagggcacc?ggacaggtcg?gtcttgacaa 10560

aaagaaccgg?gcgcccctgc?gctgacagcc?ggaacacggc?ggcatcagag?cagccgattg 10620

tctgttgtgc?ccagtcatag?ccgaatagcc?tctccaccca?agcggccgga?gaacctgcgt 10680

gcaatccatc?ttgttcaatg?gccgatccca?tattggctgc?aggtcgaaag?gcccggagat 10740

gaggaagagg?agaacagcgc?ggcagacgtg?cgcttttgaa?gcgtgcagaa?tgccgggcct 10800

ccggaggacc?ttcgggcgcc?cgccccgccc?ctgagcccgc?ccctgagccc?gcccccggac 10860

ccaccccttc?ccagcctctg?agcccagaaa?gcgaaggagc?aaagctgcta?ttggccgctg 10920

ccccaaaggc?ctacccgctt?ccattgctca?gcggtgctgt?ccatctgcac?gagactagtg 10980

agacgtgcta?cttccatttg?tcacgtcctg?cacgacgcga?gctgcggggc?gggggggaac 11040

ttcctgacta?ggggaggagt?agaaggtggc?gcgaaggggc?caccaaagaa?cggagccggt 11100

tggcgcctac?cggtggatgt?ggaatgtgtg?cgaggccaga?ggccacttgt?gtagcgccaa 11160

The target practice sequence of pPHA1

gtgcccagcg?gggctgctaa?agcgcatgct?ccagactgcc?ttgggaaaag?cgcctcccct 11220

acccggtaga?attgacctgc?aggggccctc?gactataact?tcgtatagca?tacattatac 11280

gaagttatct?cgagagttcg?atttttctat?tgttcaactt?ttattttcct?agtaaaagaa 11340

agtttcagta?aactttgaat?ctttgatgaa?ttaagttttc?ctttagcatt?tatttctaga 11400

ctgttatgat?attttgtatt?tgcgaaccct?taggaagtta?tcttattaat?atgtttctaa 11460

catgctgggt?gaaaaaatag?tgagaattgt?ttagtagcta?taattttctt?ttgcacatga 11520

aggagcttat?gtagagtgac?ttacacttca?cagagtgcag?ttgaagacag?aattaaaaat 11580

tccaaattcc?taaaggccta?ttcactgtac?cttacacctt?ggtttatatt?aagaatatac 11640

aaacacttta?taaaaagaat?ttgcatttat?atttttcatt?tcagaccatc?ctctcagtag 11700

ctggtaatag?atactaaatg?aacattagat?cttatccaag?tttgaataga?aagccataaa 11760

tatttaatga?ttttaatggt?ttttcaaata?atatcatggg?caattaaata?actcttctgt 11820

ttaaatgcaa?aagaagaaat?ttaccaccgt?cctaagattt?tctgtaaaga?tcagagccca 11880

gtatctagga?gcaaatgaat?acctaagtca?aatgaagaga?aacaaaaaat?attatcttaa 11940

ctggttgttt?cgtcagaatt?taagcaaaac?tttttttctc?tcctcttttc?cccagacaag 12000

agtgaaattg?ctcatcggtt?taaagatttg?ggagaacaat?atttcaaagg?cctgtaagtt 12060

aaaatattga?agattcaaat?ttaatgtttc?tattagtgtc?agttattatt?ttgacaccct 12120

catattcccc?cgccatcaga?gattctagag?cagtccctcc?agtagaattt?tctgcgttgg 12180

tggaaatatc?ctctattggt?gttgtctgac?agttgccatt?ggccacatgt?ggctacggag 12240

tactttaaat?atatatgacg?agtacaactg?aggatcaaaa?atttaaacag?catttacttt 12300

taatttattt?aaatctacat?cgtcacttat?ggctaatggc?aactatgttg?gacagtgtag 12360

ctctggaaat?cttgtatagc?ctgcatgggg?ggaaggattt?tgctatgtga?ttcgttcaca 12420

actcgctttc?cctagcaaat?agttctgtgg?tttcttgaga?taatttcaat?ttttttaatc 12480

taataaatag?tgagaaaaca?gtttattttt?caaggtctat?ctcatcctac?agcctgatta 12540

aatgagtgtg?tgtggttttt?tgtttgtttg?tttgtttgtt?ttaaatagtt?agcctaggta 12600

atgtcccagt?cttagtaact?aggtctattc?gaatacattt?tacctgtttg?ctctgtgtgt 12660

atgcatcttc?tgacatgata?catcttcttc?catgatagtg?gaaatataca?tccttggctt 12720

ctgaagccac?atgtacttcg?ttaagcctca?gatcacagct?aatgtgaaca?ggctttctgg 12780

ctaagagtaa?gaaagatgtg?taaaatatag?atatacagac?acacaatttg?ttttctagct 12840

The target practice sequence of pPHA1

tagcaatgta?ctgtatatta?aaattttgtt?acattttcta?cgtcctttgt?ttcagagtgc 12900

tgattgcctt?ttctcagcat?ctccagcaat?gcccatatga?agagcatgtg?aaattagtga 12960

gggaagtaac?tgagtagcaa?aaacatgtgt?tgctgatgag?tcagctgaaa?attgtgacaa 13020

gtcaattgta?agctatattc?tgctttagga?tagtatttct?tctgctttta?tgtacccttg 13080

aacatttaaa?agggaaaaag?tcccctttta?gttttctcgg?tgattactaa?gtgaccatag 13140

ttgtgagcaa?tcctcaaaag?tgggtagagc?cattatttct?tctgtcacag?agatgttcta 13200

gctataacca?caggttggtt?ttgttgttgt?tgttgtttgt?tttgttttgt?gtccttttag 13260

ggccgcacct?atggcatatg?gaggttccca?ggctgggggt?cgaatcggag?ctgtagcctc 13320

cggcctatgc?cacagctaca?gcaacaccag?atccgagccg?tgtctgtgac?ctacaccaca 13380

gcccatggca?acaccaggtc?cttaacccac?tgaacgatgc?cagggatgga?acctgcatcc 13440

tcatggatac?tagttgggtt?cattaaccac?tgagccacaa?tgagaactcc?ccattcatat 13500

ttttacatac?cataaaatat?caggactttg?aacttttgat?attttcagat?agatctacaa 13560

tatacgggta?agtttatgtt?tctctcactg?ctttatactt?agctttctgg?ggacaaggca 13620

attttttatt?tatcactgaa?ggattttctt?ctcctctata?tgtacttctt?cctcaaggct 13680

tttttttttc?attttttaaa?agttttattg?aagtagaatt?gatttagaat?gttgtgataa 13740

tttctgcttt?acaacaaagt?gattcagtta?catatctatt?cttttcagat?tcatttccca 13800

tatgaattat?cacagaatac?tgggtagaat?tccctgtgct?atacagcaga?ccccgttggc 13860

cagtcatttc?atatactata?gtgtgcattt?accaatccca?aacccccagt?ctatctctcc 13920

ctcaccccaa?catgtcctct?ttgggaacca?agcttaatca?ctagtgcggc?c 13971

The target practice sequence of pPHA2

SEQUENCE?LISTING

＜110〉Biologic Engineering Inst., Academy of Millitary Medical Sciences of P.L.A

＜120〉be used to produce the pig serum albumin gene seat targeting vector construction process of foreign protein

＜130〉the target practice sequence of pPHA2 carrier

<141>2004-06-06

<160>1

<170>PatentIn?version?3.2

<210>1

<211>14179

<212>DNA

<213>human?and?porcine

<400>1

atcgatataa?ctcaagatct?caccatctgt?tgctaggaat?attgcattag?tgccctgact 60

agtcttccta?gacatactct?tgccttctct?agtctcttcc?ccacattgca?accagagtag 120

tcttctaaag?tatagatcgg?attatattat?tgggctaata?atatcattca?acttctcaat 180

gcttttagaa?tgaagaccaa?aaatactgct?atagctaaga?tgacttccaa?atccatcttc 240

tctcatctca?cactgtgctt?ctcctcaact?ctgtgtctag?ctggtctcag?tttctttcat 300

tctctttctt?ttctcaagca?ttagcacatt?ctttcccctg?tgcttgtaag?gctattttct 360

ttcatctttc?tttatgccca?ttttaaaaag?tctcagttta?gaagtcatgt?tcctaggaaa 420

gccttctcag?acagacttcc?aggcacatgg?tattattaaa?taaatatggc?ttgaataaaa 480

aacaagtatt?tatcactaaa?tacttgggtg?gcctactgac?tttgtcagtc?ctctcaagtg 540

aactatttcc?ctataaaagt?tttgtagctt?tctgcaagaa?tatgtttctt?tgggatttat 600

aatattaaga?tgacctatct?catccttaac?tttgcctgca?aatccaaata?attgcaagtt 660

ctgcttttct?attgctgact?gccagcctgc?ctttcttttc?tttcttactc?ctgcaggaaa 720

tttcattgtc?ttctaaactg?aagtctaact?ctttgcttgc?tgcattttga?tcttttctca 780

aacatttaga?aattacacat?attcataccc?tccttacttc?cctcaatcct?ctacctactc 840

attccatttc?ttcaggtgac?aattccttca?caagcaaaaa?tgaacccaga?gcatgcaact 900

ttcaagcttt?tgtctgttaa?gttgcatagt?tagctggagt?ttagagcccg?tatatcttat 960

ttgtgtgtga?gattgtcctt?ggatttagag?cttctcctct?gatacttttt?tatcacccca 1020

ggagtccttt?gtgttctaca?tgcacagtac?tttacaatgt?tgtattgtaa?ctatcttcca 1080

The target practice sequence of pPHA2

gtgtgtctat?cttcccccta?ttaaactgcc?tgcttcatga?cctatggagg?gcttcaagaa 1140

cttaagagtc?agggactgtg?tcttgttttt?attgttttta?acctcacatc?ctaggaaatg 1200

cttggggagt?gagcacccca?tacattttga?gcatgaatga?aggggtaggt?ggctacttaa 1260

aataataagt?ctgaaacatt?gtttcagaac?tgtgcttgtc?agcaagaacg?ctgatgttcc 1320

taagtaaata?gtgcttttgt?tgatgccagg?acaatgtttg?cttttagata?ccatgattct 1380

caatgtgcat?tttttgccaa?ttttccacgt?aataatgaag?agaaatttga?actcatcaat 1440

aacggtcatt?tgcattcaag?gcattaaatg?ctgagaggct?aatttttata?attttaatga 1500

atgaatgaat?atctaatata?aatataaaaa?ttaaaatcga?attgaaataa?taattgtata 1560

tatttcatct?aataacttaa?actatttgta?ttaaataatc?taaatatttc?acaactgtga 1620

atcagaagcg?ttttggaaag?agcatgccat?tttgtcacat?tatatgctgc?tgaaattact 1680

tcttcctgct?gtttgaaagc?atatgttcat?ttcaacaggt?tattaccaaa?tagctatttt 1740

tgcaaatgga?aacttcaagc?ctctaaatat?tattctaata?aaagaatttt?gctcttttaa 1800

atgtctacat?aatgaaaaac?taatagtgaa?caattcattt?cttatatgtg?caataattat 1860

atttaagacc?atttcaaaaa?tatttatatt?gtatacttta?ttacattata?taggctagac 1920

ttggatttct?cttaaaaaaa?gaaaattatg?ccaattattt?gtttgatata?tttcttaaaa 1980

gttacaagtt?tctttggatc?aaataattca?tttcttgata?ttagccccta?catagatgct 2040

tgaaaagctg?atgagaattt?taagacttaa?agtgtaaggc?tcaggtgggg?ggcaggccag 2100

agaagtttta?gttgaagtcc?atcaatggga?ggttagaaaa?catctgataa?atttgaagct 2160

ttagaagaca?tcaaggagaa?aaaaaaatta?cccttttatg?aaaactttct?gaattttatc 2220

aaccaatgtt?tttaatatag?gactgtttta?gctacaaaac?atttgctttt?ttacaagagg 2280

ttaaaaatag?ttccatttgt?aactaatatc?cttttaagta?ataagtccct?tgaattacat 2340

ttggaaagaa?atttagaata?tgaagagact?ttttattata?ttattatatt?aaggacaatt 2400

aaacccccag?taatgtatta?aattctgttt?gatttccatc?tgttgctatt?attatgaaag 2460

agaagttact?agcattaatt?tcattttcta?tgtttgcaga?ctctgttgtt?aacttataca 2520

aagaacagat?aacatggatt?tcaggcatca?gttatatttt?tcttggattg?gaaaaatgat 2580

aaaaagatat?gagtgttatt?ttatttctat?aatttagaga?agtgaaatga?cctgttcatt 2640

tcttaagact?cttaacctga?agtagataaa?gagtgatgcc?taaggaactc?atgggcagct 2700

cagcgatgaa?cagattcgca?gatctggatc?ccaattcctt?aacttgatat?agtcattcta 2760

The target practice sequence of pPHA2

ccagtcaggc?cctttactca?ttctatgact?gtttcattgg?gaatatggaa?tcacagaatg 2820

ttcaaggcag?aagtgtcctt?aaagattatt?taatccagct?tgacccatat?ggcctaaaga 2880

tatagacaga?tctgcacact?tgagtttgca?gctagaaatt?gacattgaga?tgaattaaac 2940

tgcatagtaa?agagttgaga?agttttaagt?aaatctgaga?gaaagtagac?ctaaattcta 3000

gatgcccagt?gtagcatgag?gaaagatgga?aaagggaccc?tcctgccatg?ggcgctttgg 3060

agagatctgg?atttaagaga?tgggacaagc?caaggatggt?aatttagtgt?ggtttttata 3120

atgtaaactt?tcttcccttt?atcccctatg?attttttcag?caaagatttt?tctcactggg 3180

attcctgcca?tggcacattg?gaaatgaatc?cgactaggaa?ccatgagatt?atgggtttga 3240

tccctggcct?cgctcagtgg?gttaaggatc?tggggttgcc?gtgagctatg?gtgtaggttg 3300

cagatgtggc?tcagatctgg?cattgctgtg?gctgtggtgt?aggccggcag?ttgtatctcc 3360

gattagaccc?ctagcctggg?aatcaccacg?tcgcgggtgt?ggtcctaaaa?agacaaaagg 3420

ccaaaaaaaa?aaaaaaaaaa?aaaaaagatt?tttctcacta?atgcaagtgt?ttatggaggg 3480

ctgtgaaggg?acaattgata?tttgaattac?attacaaatt?ttatgtttgc?attagaaatg 3540

cctatattgt?tcacacaatt?tggacaattt?attttctttt?attaatggga?aatgtatctt 3600

cataagtgaa?taagaaatta?aattgcttac?catatttcaa?ttagtttgat?gcctaatact 3660

ctttcacttt?tctatccttc?tgtgttttta?gaaagcagtc?caggctcttc?aatgataaat 3720

aagatctatc?ctacctatat?gtggacatcc?acacatatgt?cccatgcata?ataggaagaa 3780

tgttaagaaa?cgatttttct?gtttaaattt?ccctccaaat?aaatccaaat?ttcccacatt 3840

ttggagtgac?tttgggttaa?atgtttctag?aaagtctgtg?ttgtaatact?gaggctatta 3900

tcagtcattg?ttgtcccatt?ttaatgcata?tgtttccctt?gatttctaac?tgaaggcaac 3960

ccagaagtaa?taatctatta?cagaaggaga?tggcataaag?gttgaagaag?gaaaatggac 4020

aaatggaggg?aaacctggtt?tgaatgactt?ggtggaagct?gcagggatcc?cgaaggtagc 4080

aggagggtat?aggtttaagt?ttttgattag?gatggcaagg?aagtggggga?ctggtccaag 4140

agatacttca?tctgcttcaa?atctgtgcct?tttctttcct?gtcaaaatga?atgcaaaaaa 4200

tgaaggacaa?ggggataaaa?tatttcaact?gaggtatttt?tttctcttct?aaatgatcag 4260

catataatta?actctttcca?taagaagtaa?tcttcttaac?tactctctct?ttaaacttct 4320

cccataaaat?taggaacaca?ttgaaaaatt?attggagcta?gtttcctatt?tatttagaag 4380

tagtttctat?aagtcatatt?taaaactctt?ggtttaaata?ttcacattta?ctcttgtgcc 4440

The target practice sequence of pPHA2

agattcctta?ggtgactaac?taaatacaag?ggtttaaaat?cttgaagaac?agaatactta 4500

gaaataaaca?acaattaagt?aattttattc?atctcagttt?agaatatata?ctcagaatta 4560

atagaagaaa?aatgtaggta?cccttacttt?gggaacctac?aagatgcaaa?tattcaaggg 4620

tttcttaaaa?gttaataatt?ttaaagcaga?tagtgttaag?aattaaatat?tttaatttct 4680

caaccatctg?ctaatcttta?aaaaagatac?atacacagaa?atttcattat?gctattcaca 4740

ggaaacattc?ttttttaaaa?tgagttttat?tttttccatt?atagttggtt?atagttttct 4800

cctgattttc?tactgtacag?caaagtgacc?cagtcacaca?tacatatata?ccttcttttt 4860

ctcacattat?cctccatcat?gctccatcac?aaatgactgg?atatagttcc?cagtgctata 4920

caccaggctc?tcattgctta?ttcattccaa?atgcaatagt?ttgcatctat?taaccccaga 4980

ttcccagtcc?atcccactcc?ctccccctcc?cccttggcaa?ccacaagtct?gttctccaag 5040

tccatgagtt?tcttctctgt?ggaaagtttc?atttgtgtcg?tatattagat?tccagatata 5100

aggaaataac?ctttcaggtg?ttttcagttt?tatatttgaa?aactctgtga?tcttcattaa 5160

atataagaat?atcaatttta?agtttctaaa?attgtaatga?atttgatttg?gggggttttc 5220

tgtatttacc?attttattac?aattgcagtg?caaagtaggt?ggagagacta?atatttttct 5280

tttcatttgt?caaataaaaa?atatgcacga?agtatatgtg?tgagtgacaa?aagcctgatt 5340

ctgtgaatta?gtaatacagt?tatatccaag?gataaggact?gcgtatttaa?taaagccttc 5400

ccagaggact?catgaggaac?actgacttca?aataaaagat?gcctttttct?cagaagctca 5460

aagttgatat?aatttttttt?taaatttaga?aaattagaat?agctgccttt?tttctcttct 5520

agtattggat?tttaggcttt?aaaaaatgat?tttctccaat?ggaaaaaatt?ccatagacag 5580

acattcactt?atttctgttc?agctgtgccc?agaaagacaa?gtctatttct?gttatttatg 5640

gagccattaa?aaaaaaaaaa?tctatcagct?ctgtctagct?aggtagctaa?tcatctatct 5700

gtttgtatta?tctacctctg?taatctatca?aaatattgtc?ttattgcctg?ctttcctatc 5760

atctcctctt?ttgtgcttga?ctatttaatt?agtacccata?cctgagaagt?gaaaaaaaaa 5820

gtcatagaaa?ataaatttct?tacatcatat?gactgaatag?gaaaaataat?acttctttta 5880

agtatttact?tgtgtctgtg?ccatatgtgc?cacagagtga?catggctgac?agtttgattg 5940

acttggttat?ttaattaact?gaaatgactg?gcttgatact?aattattgtt?ctgtagtatc 6000

aaagtatcag?aggaggttag?ttttcctttt?tcttttgatt?gaaatccgta?gttcttcttg 6060

gctgtgtcga?tgacatgtgg?aagttcctgg?accagggatc?taacgggtgc?tgcagctgca 6120

The target practice sequence of pPHA2

accagtgcca?cagctgtgga?aacgccttaa?cccattgcac?caccagggag?cttcatgaaa 6180

tccatagttc?ttcatcttta?gagcaaaagg?gcagagagag?aatagtcctt?cagaggcaga 6240

gatcagaatt?tggggtggga?aaagtaagga?aggaagttcg?aaagaacatt?tttgaaaaat 6300

tatctttgtt?tttagacatg?aagttaaatt?tatttcgaaa?tttaaaataa?cattgaagaa 6360

gagcatgtga?ctttcctttt?tagtaaaagc?gagaaagaaa?gaaaaatgtg?aaatagggat 6420

ggaaagaata?taatgcctgg?taaagaaggt?ctaggattct?taaaaccact?aatataatca 6480

ggctcagcct?gccctgtgta?tatcatgata?gaaaagatta?ccactgctgc?cattgtgtcc 6540

attttcttcc?ccatatctcc?atgagaggta?ccatactgcc?tttaagtact?ttactctaga 6600

gcctgaatca?ccagtggcca?gagctgaggt?aaaaatgtag?gacttctcaa?catccgtttt 6660

agttgttatt?gttgctatga?ctttttctta?gtttgcattt?gagacactgg?tttttttgac 6720

agaacagttt?cagaagaatg?aaaagccatg?gaagccctcg?aatgcgtgca?tgcatgcatg 6780

catgcatgca?cacacccatg?tgtgtatgca?ttttcactac?ttatcaataa?aagcatactt 6840

ggaattaata?atagatacaa?ttcagagctt?agctctttta?acatgtctta?tttccaagct 6900

aagtcactgg?tttgcttaaa?tttttgcaaa?ttttgaaaac?taacacgttc?aaatggtaaa 6960

ccacagatag?agatcatggt?aatagtacaa?atcgtttaaa?ctcttatgta?aaatttgata 7020

aggtgtttta?cataacttta?atgaattgac?aaggtcttgt?ggagaaaaca?gatccagacg 7080

gcaaacatac?gcaagggatt?tagtcaaaca?catttttggc?aaaaaaacta?tgaattttgt 7140

aatcagttgt?gagccaatga?aatacaaaaa?tgagtctagt?taataatcta?caattattgg 7200

ttaaagaagt?atattagtgc?tgactttcct?ctgttcgtcc?taccttttct?cttctatcaa 7260

ccccacaagc?ctttggcaca?atgaagtggg?taacctttat?ttcccttctt?tttctcttta 7320

gctcggctta?ttccaggggt?gtgtttcgtc?gagatgcaca?caagagtgag?gttgctcatc 7380

ggtttaaaga?tttgggagaa?gaaaatttca?aagccttggt?gttgattgcc?tttgctcagt 7440

atcttcagca?gtgtccattt?gaagatcatg?taaaattagt?gaatgaagta?actgaatttg 7500

caaaaacatg?tgtagctgat?gagtcagctg?aaaattgtga?caaatcactt?catacccttt 7560

ttggagacaa?attatgcaca?gttgcaactc?ttcgtgaaac?ctatggtgaa?atggctgact 7620

gctgtgcaaa?acaagaacct?gagagaaatg?aatgcttctt?gcaacacaaa?gatgacaacc 7680

caaacctccc?ccgattggtg?agaccagagg?ttgatgtgat?gtgcactgct?tttcatgaca 7740

atgaagagac?atttttgaaa?aaatacttat?atgaaattgc?cagaagacat?ccttactttt 7800

The target practice sequence of pPHA2

atgccccgga?actccttttc?tttgctaaaa?ggtataaagc?tgcttttaca?gaatgttgcc 7860

aagctgctga?taaagctgcc?tgcctgttgc?caaagctcga?tgaacttcgg?gatgaaggga 7920

aggcttcgtc?tgccaaacag?agactcaaat?gtgccagtct?ccaaaaattt?ggagaaagag 7980

ctttcaaagc?atgggcagtg?gctcgcctga?gccagagatt?tcccaaagct?gagtttgcag 8040

aagtttccaa?gttagtgaca?gatcttacca?aagtccacac?ggaatgctgc?catggagatc 8100

tgcttgaatg?tgctgatgac?agggcggacc?ttgccaagta?tatctgtgaa?aatcaggatt 8160

cgatctccag?taaactgaag?gaatgctgtg?aaaaacctct?gttggaaaaa?tcccactgca 8220

ttgccgaagt?ggaaaatgat?gagatgcctg?ctgacttgcc?ttcattagct?gctgattttg 8280

ttgaaagtaa?ggatgtttgc?aaaaactatg?ctgaggcaaa?ggatgtcttc?ctgggcatgt 8340

ttttgtatga?atatgcaaga?aggcatcctg?attactctgt?cgtgctgctg?ctgagacttg 8400

ccaagacata?tgaaaccact?ctagagaagt?gctgtgccgc?tgcagatcct?catgaatgct 8460

atgccaaagt?gttcgatgaa?tttaaacctc?ttgtggaaga?gcctcagaat?ttaatcaaac 8520

aaaactgtga?gctttttaag?cagcttggag?agtacaaatt?ccagaatgcg?ctattagttc 8580

gttacaccaa?gaaagtaccc?caagtgtcaa?ctccaactct?tgtagaggtc?tcaagaaacc 8640

taggaaaagt?gggcagcaaa?tgttgtaaac?atcctgaagc?aaaaagaatg?ccctgtgcag 8700

aagactatct?atccgtggtc?ctgaaccagt?tatgtgtgtt?gcatgagaaa?acgccagtaa 8760

gtgacagagt?cacaaaatgc?tgcacagagt?ccttggtgaa?caggcgacca?tgcttttcag 8820

ctctggaagt?cgatgaaaca?tacgttccca?aagagtttaa?tgctgaaaca?ttcaccttcc 8880

atgcagatat?atgcacactt?tctgagaagg?agagacaaat?caagaaacaa?actgcacttg 8940

ttgagcttgt?gaaacacaag?cccaaggcaa?caaaagagca?actgaaagct?gttatggatg 9000

atttcgcagc?ttttgtagag?aagtgctgca?aggctgacga?taaggagacc?tgctttgccg 9060

aggagggtaa?aaaacttgtt?gctgcaagtc?aagctgcctt?aggcttataa?gtcgaccaaa 9120

cccgctgatc?agcctcgact?gtgccttcta?gttgccagcc?atctgttgtt?tgcccctccc 9180

ccgtgccttc?cttgaccctg?gaaggtgcca?ctcccactgt?cctttcctaa?taaaatgagg 9240

aaattgcatc?gcattgtctg?agtaggtgtc?attctattct?ggggggtggg?gtggggcagg 9300

acagcaaggg?ggaggattgg?gaagacaata?gcaggcatgc?tggggatgcg?gtgggctcta 9360

tggccgcgga?caacacagtg?acaagcatct?caggtaaggt?accataactt?cgtatagcat 9420

acattatacg?aagttatatt?gctagagtca?gcttctgatg?gaattagaac?ttggcaaaac 9480

The target practice sequence of pPHA2

aatactgaga?atgaagtgta?tgtggaacag?aggctgctga?tctcgttctt?caggctatga 9540

aactgacaca?tttggaaacc?acagtactta?gaaccacaaa?gtgggaatca?agagaaaaac 9600

aatgatccca?cgagagatct?atagatctat?agatcatgag?tgggaggaat?gagctggccc 9660

ttaatttggt?tttgcttgtt?taaattatga?tatccaacta?tgaaacatta?tcataaagca 9720

atagtaaaga?gccttcagta?aagagcaggc?atttatctaa?tcccacccca?cccccacccc 9780

cgtagctcca?atccttccat?tcaaaatgta?ggtactctgt?tctcaccctt?cttaacaaag 9840

tatgacagga?aaaacttcca?ttttagtgga?catctttatt?gtttaataga?tcatcaattt 9900

gtgcagactt?acagcggatc?gatcccctca?gaagaactcg?tcaagaaggc?gatagaaggc 9960

gatgcgctgc?gaatcgggag?cggcgatacc?gtaaagcacg?aggaagcggt?cagcccattc 10020

gccgccaagc?tcttcagcaa?tatcacgggt?agccaacgct?atgtcctgat?agcggtccgc 10080

cacacccagc?cggccacagt?cgatgaatcc?agaaaagcgg?ccattttcca?ccatgatatt 10140

cggcaagcag?gcatcgccat?gggtcacgac?gagatcctcg?ccgtcgggca?tgcgcgcctt 10200

gagcctggcg?aacagttcgg?ctggcgcgag?cccctgatgc?tcttcgtcca?gatcatcctg 10260

atcgacaaga?ccggcttcca?tccgagtacg?tgctcgctcg?atgcgatgtt?tcgcttggtg 10320

gtcgaatggg?caggtagccg?gatcaagcgt?atgcagccgc?cgcattgcat?cagccatgat 10380

ggatactttc?tcggcaggag?caaggtgaga?tgacaggaga?tcctgccccg?gcacttcgcc 10440

caatagcagc?cagtcccttc?ccgcttcagt?gacaacgtcg?agcacagctg?cgcaaggaac 10500

gcccgtcgtg?gccagccacg?atagccgcgc?tgcctcgtcc?tgcagttcat?tcagggcacc 10560

ggacaggtcg?gtcttgacaa?aaagaaccgg?gcgcccctgc?gctgacagcc?ggaacacggc 10620

ggcatcagag?cagccgattg?tctgttgtgc?ccagtcatag?ccgaatagcc?tctccaccca 10680

agcggccgga?gaacctgcgt?gcaatccatc?ttgttcaatg?gccgatccca?tattggctgc 10740

aggtcgaaag?gcccggagat?gaggaagagg?agaacagcgc?ggcagacgtg?cgcttttgaa 10800

gcgtgcagaa?tgccgggcct?ccggaggacc?ttcgggcgcc?cgccccgccc?ctgagcccgc 10860

ccctgagccc?gcccccggac?ccaccccttc?ccagcctctg?agcccagaaa?gcgaaggagc 10920

aaagctgcta?ttggccgctg?ccccaaaggc?ctacccgctt?ccattgctca?gcggtgctgt 10980

ccatctgcac?gagactagtg?agacgtgcta?cttccatttg?tcacgtcctg?cacgacgcga 11040

gctgcggggc?gggggggaac?ttcctgacta?ggggaggagt?agaaggtggc?gcgaaggggc 11100

caccaaagaa?cggagccggt?tggcgcctac?cggtggatgt?ggaatgtgtg?cgaggccaga 11160

The target practice sequence of pPHA2

ggccacttgt?gtagcgccaa?gtgcccagcg?gggctgctaa?agcgcatgct?ccagactgcc 11220

ttgggaaaag?cgcctcccct?acccggtaga?attgacctgc?aggggccctc?gactataact 11280

tcgtatagca?tacattatac?gaagttatct?cgagctatac?tttatatttt?ttttttctta 11340

aaagtagctg?tggggagttc?ctgtcgtggc?gcagtggtta?acgaacccga?ctaggaacta 11400

tgaggttgcg?ggttcggtcc?ctgcccttgc?tcagtgggtt?aacgatccgg?cattgccgtg 11460

agctgtggtg?taggttgcag?acgcggctcg?gatccagcgt?tgctgtggct?ctggcgtagg 11520

ccggtggctg?cagctccgat?tagaccccta?gcctgggaac?ctccatatgc?cgtgggagcg 11580

gcccaagaaa?tagcaaaaag?acaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaagta 11640

gctgtggaat?tccctggtgg?ctcagcgagt?taagggtcca?gcatttttgc?tgctgtggct 11700

ctgattactg?ttgtggggca?ggtttgatcc?ctggcctagg?agcttctgca?gaccgcaggc 11760

acagccaaag?gggaaaaaaa?agtaattata?atacttatta?ttgaaagcaa?agagtgactg 11820

accagtgcca?gtcactattc?taaactcatt?atatatatta?acttctttaa?ttctcaaaat 11880

atttgcctga?gttgaatacc?attattattc?tccttttgca?aatgagaaga?cttctcctgt 11940

ggctcacatc?ataaccattt?ctcaaatgag?gaaaagaagg?cacagaacaa?ggcctaggct 12000

aaataattta?cctaaggacc?actcaactag?tgagaggtgg?ggccaagatg?caaaattcaa 12060

accagggctt?gagagctcac?cactgtcctt?tgtgacctaa?tgtggtgcag?aagagtcatt 12120

taactaagca?ctggctcaac?agtgggttac?tttaggtaga?ggctagacta?aatgcaagtg 12180

catttctaca?tgtaaaaacc?caactgaata?tcactgattt?cacatggtta?accagatagc 12240

tcaagaacaa?attcatttca?ctggaggatg?tgatagttca?acctgtgtga?acttctactc 12300

taagtaaata?atttgctaca?gtttccaatg?tgttgacatg?ctgggttttt?tgtttgtttg 12360

tttgtttttc?ggtctttttg?ccatttctaa?ggccgctcct?gtggcatatg?gaggtgccta 12420

ggctagggat?ccaattggag?ctgcagctgc?tggcctacac?cacagccaca?gcaatgcggg 12480

atccgagctg?tgtctgcaac?ctacaccaca?gctcacggca?acgccggatc?ctttaaccca 12540

ctgagcaagg?gcagggaccg?aacccacaac?ctcatggttc?ctagttggat?ttgttaacca 12600

ctgcgccact?acgggaaccc?cgagaacttc?ttttcttatg?acaaaagatc?ggctttaaaa 12660

tttagatgtt?tgaacataaa?catgttttac?ccattttggt?tataatattt?tttgagaagt 12720

taaaaaaata?agatgaactt?ccttttttga?aactagttgt?ccagctatat?tttattttat 12780

tatgttttgg?ccacctcaca?gcatatggag?ctcccgggcc?agggatgaga?tctgagctac 12840

The target practice sequence of pPHA2

agtcattcgc?aacctaagcc?atagctgtgg?aaatgctgga?tttttcaccc?actcagacag 12900

gctggggatt?gaacctgcat?cccggccttc?ccaagacact?gccaatccct?tcatgccaca 12960

gtaggaactc?catccaacta?ttttttagca?tgaataattt?tcatattttt?caaactgatt 13020

gtgatgttta?ttccactgtg?aggttttaac?cttgctcttt?gctaatagga?taatacttat 13080

aattgtgcat?tcggccaaag?aaccaacttc?agatctctgc?aaatttgggc?atttcaaaat 13140

gtgatttgtc?ttagtcctaa?atggcaagac?tttcttccta?gaggtctaac?ttggagaaga 13200

cacctttttt?gtttgtaatt?aacttagtga?gaagtagatg?ttaagttaat?gactctgagg 13260

gtcgatgagg?aaaaggctga?ggagtccaat?gacactggtg?tcaaagagag?gactttacaa 13320

tccttatagc?ccaacagaag?aatttagccg?ccatgagtct?aggacagcct?taaattgttt 13380

tcactggtgg?aaattacaga?aagacagtct?aagcaattta?gcattcattt?gaatagattt 13440

gagaaaacac?acagcatttt?ataaacagta?attatattta?tccttttgtt?tttcagacta 13500

ccctgagaat?aagagaaaga?gaaatgaaga?cctagactta?tccatctctt?tttcttttct 13560

gttggtttta?aaccaacacc?ctgtctaaag?tacacaaatt?tctttaaata?ttttgcctct 13620

tttctctgtg?ctacaattaa?taaaaaaatg?aaaagaatct?aatttaattg?tctatgactg 13680

ttattttttt?gaagatgtgt?tgtcaacctg?ataattttgt?aggttctatg?aaaattccac 13740

tattctctct?ttccctattt?caatggagga?cttctagttc?cttctggatt?aattgcataa 13800

aagaaacatt?aatactgcca?tgagttatgg?attatgaaca?ttcaaagtaa?tattttaaca 13860

ttatgataat?tctgaataaa?agagtagagc?ctgtggtata?ggtgagacat?ataaaacatg 13920

gtttatggag?ttcccttcat?ggcacagtgg?aaacgaatct?gactaggaac?catggaggtt 13980

tcgggttcaa?tccctggcct?cactcagtgg?gttaaggatc?cagtgttgct?gtgagctgtg 14040

gtatagtttg?caaacttggc?tcgtatctgg?cattgctgtg?gctttggcct?aggccggcag 14100

ctgtagctcc?gatttgaccc?ttggcctggg?aaccatcata?tgcagcagaa?gaggcccaag 14160

cttaatcact?agtgcggcc 14179

The target practice sequence of pPHA3

SEQUENCE?LISTING

＜110〉Biologic Engineering Inst., Academy of Millitary Medical Sciences of P.L.A

＜120〉be used to produce the pig serum albumin gene seat targeting vector construction process of foreign protein

＜130〉the target practice sequence of pPHA1 carrier

<141>2004-06-06

<160>1

<170>PatentIn?version?3.2

<210>1

<211>27964

<212>DNA

<213>human?and?porcine

<400>1

atcgatataa?ctcaagatct?caccatctgt?tgctaggaat?attgcattag?tgccctgact 60

agtcttccta?gacatactct?tgccttctct?agtctcttcc?ccacattgca?accagagtag 120

tcttctaaag?tatagatcgg?attatattat?tgggctaata?atatcattca?acttctcaat 180

gcttttagaa?tgaagaccaa?aaatactgct?atagctaaga?tgacttccaa?atccatcttc 240

tctcatctca?cactgtgctt?ctcctcaact?ctgtgtctag?ctggtctcag?tttctttcat 300

tctctttctt?ttctcaagca?ttagcacatt?ctttcccctg?tgcttgtaag?gctattttct 360

ttcatctttc?tttatgccca?ttttaaaaag?tctcagttta?gaagtcatgt?tcctaggaaa 420

gccttctcag?acagacttcc?aggcacatgg?tattattaaa?taaatatggc?ttgaataaaa 480

aacaagtatt?tatcactaaa?tacttgggtg?gcctactgac?tttgtcagtc?ctctcaagtg 540

aactatttcc?ctataaaagt?tttgtagctt?tctgcaagaa?tatgtttctt?tgggatttat 600

aatattaaga?tgacctatct?catccttaac?tttgcctgca?aatccaaata?attgcaagtt 660

ctgcttttct?attgctgact?gccagcctgc?ctttcttttc?tttcttactc?ctgcaggaaa 720

tttcattgtc?ttctaaactg?aagtctaact?ctttgcttgc?tgcattttga?tcttttctca 780

aacatttaga?aattacacat?attcataccc?tccttacttc?cctcaatcct?ctacctactc 840

attccatttc?ttcaggtgac?aattccttca?caagcaaaaa?tgaacccaga?gcatgcaact 900

ttcaagcttt?tgtctgttaa?gttgcatagt?tagctggagt?ttagagcccg?tatatcttat 960

ttgtgtgtga?gattgtcctt?ggatttagag?cttctcctct?gatacttttt?tatcacccca 1020

ggagtccttt?gtgttctaca?tgcacagtac?tttacaatgt?tgtattgtaa?ctatcttcca 1080

The target practice sequence of pPHA3

gtgtgtctat?cttcccccta?ttaaactgcc?tgcttcatga?cctatggagg?gcttcaagaa 1140

cttaagagtc?agggactgtg?tcttgttttt?attgttttta?acctcacatc?ctaggaaatg 1200

cttggggagt?gagcacccca?tacattttga?gcatgaatga?aggggtaggt?ggctacttaa 1260

aataataagt?ctgaaacatt?gtttcagaac?tgtgcttgtc?agcaagaacg?ctgatgttcc 1320

taagtaaata?gtgcttttgt?tgatgccagg?acaatgtttg?cttttagata?ccatgattct 1380

caatgtgcat?tttttgccaa?ttttccacgt?aataatgaag?agaaatttga?actcatcaat 1440

aacggtcatt?tgcattcaag?gcattaaatg?ctgagaggct?aatttttata?attttaatga 1500

atgaatgaat?atctaatata?aatataaaaa?ttaaaatcga?attgaaataa?taattgtata 1560

tatttcatct?aataacttaa?actatttgta?ttaaataatc?taaatatttc?acaactgtga 1620

atcagaagcg?ttttggaaag?agcatgccat?tttgtcacat?tatatgctgc?tgaaattact 1680

tcttcctgct?gtttgaaagc?atatgttcat?ttcaacaggt?tattaccaaa?tagctatttt 1740

tgcaaatgga?aacttcaagc?ctctaaatat?tattctaata?aaagaatttt?gctcttttaa 1800

atgtctacat?aatgaaaaac?taatagtgaa?caattcattt?cttatatgtg?caataattat 1860

atttaagacc?atttcaaaaa?tatttatatt?gtatacttta?ttacattata?taggctagac 1920

ttggatttct?cttaaaaaaa?gaaaattatg?ccaattattt?gtttgatata?tttcttaaaa 1980

gttacaagtt?tctttggatc?aaataattca?tttcttgata?ttagccccta?catagatgct 2040

tgaaaagctg?atgagaattt?taagacttaa?agtgtaaggc?tcaggtgggg?ggcaggccag 2100

agaagtttta?gttgaagtcc?atcaatggga?ggttagaaaa?catctgataa?atttgaagct 2160

ttagaagaca?tcaaggagaa?aaaaaaatta?cccttttatg?aaaactttct?gaattttatc 2220

aaccaatgtt?tttaatatag?gactgtttta?gctacaaaac?atttgctttt?ttacaagagg 2280

ttaaaaatag?ttccatttgt?aactaatatc?cttttaagta?ataagtccct?tgaattacat 2340

ttggaaagaa?atttagaata?tgaagagact?ttttattata?ttattatatt?aaggacaatt 2400

aaacccccag?taatgtatta?aattctgttt?gatttccatc?tgttgctatt?attatgaaag 2460

agaagttact?agcattaatt?tcattttcta?tgtttgcaga?ctctgttgtt?aacttataca 2520

aagaacagat?aacatggatt?tcaggcatca?gttatatttt?tcttggattg?gaaaaatgat 2580

aaaaagatat?gagtgttatt?ttatttctat?aatttagaga?agtgaaatga?cctgttcatt 2640

tcttaagact?cttaacctga?agtagataaa?gagtgatgcc?taaggaactc?atgggcagct 2700

cagcgatgaa?cagattcgca?gatctggatc?ccaattcctt?aacttgatat?agtcattcta 2760

The target practice sequence of pPHA3

ccagtcaggc?cctttactca?ttctatgact?gtttcattgg?gaatatggaa?tcacagaatg 2820

ttcaaggcag?aagtgtcctt?aaagattatt?taatccagct?tgacccatat?ggcctaaaga 2880

tatagacaga?tctgcacact?tgagtttgca?gctagaaatt?gacattgaga?tgaattaaac 2940

tgcatagtaa?agagttgaga?agttttaagt?aaatctgaga?gaaagtagac?ctaaattcta 3000

gatgcccagt?gtagcatgag?gaaagatgga?aaagggaccc?tcctgccatg?ggcgctttgg 3060

agagatctgg?atttaagaga?tgggacaagc?caaggatggt?aatttagtgt?ggtttttata 3120

atgtaaactt?tcttcccttt?atcccctatg?attttttcag?caaagatttt?tctcactggg 3180

attcctgcca?tggcacattg?gaaatgaatc?cgactaggaa?ccatgagatt?atgggtttga 3240

tccctggcct?cgctcagtgg?gttaaggatc?tggggttgcc?gtgagctatg?gtgtaggttg 3300

cagatgtggc?tcagatctgg?cattgctgtg?gctgtggtgt?aggccggcag?ttgtatctcc 3360

gattagaccc?ctagcctggg?aatcaccacg?tcgcgggtgt?ggtcctaaaa?agacaaaagg 3420

ccaaaaaaaa?aaaaaaaaaa?aaaaaagatt?tttctcacta?atgcaagtgt?ttatggaggg 3480

ctgtgaaggg?acaattgata?tttgaattac?attacaaatt?ttatgtttgc?attagaaatg 3540

cctatattgt?tcacacaatt?tggacaattt?attttctttt?attaatggga?aatgtatctt 3600

cataagtgaa?taagaaatta?aattgcttac?catatttcaa?ttagtttgat?gcctaatact 3660

ctttcacttt?tctatccttc?tgtgttttta?gaaagcagtc?caggctcttc?aatgataaat 3720

aagatctatc?ctacctatat?gtggacatcc?acacatatgt?cccatgcata?ataggaagaa 3780

tgttaagaaa?cgatttttct?gtttaaattt?ccctccaaat?aaatccaaat?ttcccacatt 3840

ttggagtgac?tttgggttaa?atgtttctag?aaagtctgtg?ttgtaatact?gaggctatta 3900

tcagtcattg?ttgtcccatt?ttaatgcata?tgtttccctt?gatttctaac?tgaaggcaac 3960

ccagaagtaa?taatctatta?cagaaggaga?tggcataaag?gttgaagaag?gaaaatggac 4020

aaatggaggg?aaacctggtt?tgaatgactt?ggtggaagct?gcagggatcc?cgaaggtagc 4080

aggagggtat?aggtttaagt?ttttgattag?gatggcaagg?aagtggggga?ctggtccaag 4140

agatacttca?tctgcttcaa?atctgtgcct?tttctttcct?gtcaaaatga?atgcaaaaaa 4200

tgaaggacaa?ggggataaaa?tatttcaact?gaggtatttt?tttctcttct?aaatgatcag 4260

catataatta?actctttcca?taagaagtaa?tcttcttaac?tactctctct?ttaaacttct 4320

cccataaaat?taggaacaca?ttgaaaaatt?attggagcta?gtttcctatt?tatttagaag 4380

tagtttctat?aagtcatatt?taaaactctt?ggtttaaata?ttcacattta?ctcttgtgcc 4440

The target practice sequence of pPHA3

agattcctta?ggtgactaac?taaatacaag?ggtttaaaat?cttgaagaac?agaatactta 4500

gaaataaaca?acaattaagt?aattttattc?atctcagttt?agaatatata?ctcagaatta 4560

atagaagaaa?aatgtaggta?cccttacttt?gggaacctac?aagatgcaaa?tattcaaggg 4620

tttcttaaaa?gttaataatt?ttaaagcaga?tagtgttaag?aattaaatat?tttaatttct 4680

caaccatctg?ctaatcttta?aaaaagatac?atacacagaa?atttcattat?gctattcaca 4740

ggaaacattc?ttttttaaaa?tgagttttat?tttttccatt?atagttggtt?atagttttct 4800

cctgattttc?tactgtacag?caaagtgacc?cagtcacaca?tacatatata?ccttcttttt 4860

ctcacattat?cctccatcat?gctccatcac?aaatgactgg?atatagttcc?cagtgctata 4920

caccaggctc?tcattgctta?ttcattccaa?atgcaatagt?ttgcatctat?taaccccaga 4980

ttcccagtcc?atcccactcc?ctccccctcc?cccttggcaa?ccacaagtct?gttctccaag 5040

tccatgagtt?tcttctctgt?ggaaagtttc?atttgtgtcg?tatattagat?tccagatata 5100

aggaaataac?ctttcaggtg?ttttcagttt?tatatttgaa?aactctgtga?tcttcattaa 5160

atataagaat?atcaatttta?agtttctaaa?attgtaatga?atttgatttg?gggggttttc 5220

tgtatttacc?attttattac?aattgcagtg?caaagtaggt?ggagagacta?atatttttct 5280

tttcatttgt?caaataaaaa?atatgcacga?agtatatgtg?tgagtgacaa?aagcctgatt 5340

ctgtgaatta?gtaatacagt?tatatccaag?gataaggact?gcgtatttaa?taaagccttc 5400

ccagaggact?catgaggaac?actgacttca?aataaaagat?gcctttttct?cagaagctca 5460

aagttgatat?aatttttttt?taaatttaga?aaattagaat?agctgccttt?tttctcttct 5520

agtattggat?tttaggcttt?aaaaaatgat?tttctccaat?ggaaaaaatt?ccatagacag 5580

acattcactt?atttctgttc?agctgtgccc?agaaagacaa?gtctatttct?gttatttatg 5640

gagccattaa?aaaaaaaaaa?tctatcagct?ctgtctagct?aggtagctaa?tcatctatct 5700

gtttgtatta?tctacctctg?taatctatca?aaatattgtc?ttattgcctg?ctttcctatc 5760

atctcctctt?ttgtgcttga?ctatttaatt?agtacccata?cctgagaagt?gaaaaaaaaa 5820

gtcatagaaa?ataaatttct?ttcatcatat?gactgaatag?gaaaaataat?acttctttta 5880

agtatttact?tgtgtctgtg?ccatatgtgc?cacagagtga?catggctgac?agtttgattg 5940

acttggttat?ttaattaact?gaaatgactg?gcttgatact?aattattgtt?ctgtagtatc 6000

aaagtatcag?aggaggttag?ttttcctttt?tcttttgatt?gaaatccgta?gttcttcttg 6060

gctgtgtcga?tgacatgtgg?aagttcctgg?accagggatc?taacgggtgc?tgcagctgca 6120

The target practice sequence of pPHA3

accagtgcca?cagctgtgga?aacgccttaa?cccattgcac?caccagggag?cttcatgaaa 6180

tccatagttc?ttcatcttta?gagcaaaagg?gcagagagag?aatagtcctt?cagaggcaga 6240

gatcagaatt?tggggtggga?aaagtaagga?aggaagttcg?aaagaacatt?tttgaaaaat 6300

tatctttgtt?tttagacatg?aagttaaatt?tatttcgaaa?tttaaaataa?cattgaagaa 6360

gagcatgtga?ctttcctttt?tagtaaaagc?gagaaagaaa?gaaaaatgtg?aaatagggat 6420

ggaaagaata?taatgcctgg?taaagaaggt?ctaggattct?taaaaccact?aatataatca 6480

ggctcagcct?gccctgtgta?tatcatgata?gaaaagatta?ccactgctgc?cattgtgtcc 6540

attttcttcc?ccatatctcc?atgagaggta?ccatactgcc?tttaagtact?ttactctaga 6600

gcctgaatca?ccagtggcca?gagctgaggt?aaaaatgtag?gacttctcaa?catccgtttt 6660

agttgttatt?gttgctatga?ctttttctta?gtttgcattt?gagacactgg?tttttttgac 6720

agaacagttt?cagaagaatg?aaaagccatg?gaagccctcg?aatgcgtgca?tgcatgcatg 6780

catgcatgca?cacacccatg?tgtgtatgca?ttttcactac?ttatcaataa?aagcatactt 6840

ggaattaata?atagatacaa?ttcagagctt?agctctttta?acatgtctta?tttccaagct 6900

aagtcactgg?tttgcttaaa?tttttgcaaa?ttttgaaaac?taacacgttc?aaatggtaaa 6960

ccacagatag?agatcatggt?aatagtacaa?atcgtttaaa?ctcttatgta?aaatttgata 7020

aggtgtttta?cataacttta?atgaattgac?aaggtcttgt?ggagaaaaca?gatccagacg 7080

gcaaacatac?gcaagggatt?tagtcaaaca?catttttggc?aaaaaaacta?tgaattttgt 7140

aatcagttgt?gagccaatga?aatacaaaaa?tgagtctagt?taataatcta?caattattgg 7200

ttaaagaagt?atattagtgc?tgactttcct?ctgttcgtcc?taccttttct?cttctatcaa 7260

ccccacaagc?ctttggcaca?atgcacgtaa?gaaatccatt?tttctattgt?tcaactttta 7320

ttctattttc?ccagtaaaat?aaagttttag?taaactctgc?atctttaaag?aattattttg 7380

gcatttattt?ctaaaatggc?atagtatttt?gtatttgtga?agtcttacaa?ggttatctta 7440

ttaataaaat?tcaaacatcc?taggtaaaaa?aaaaaaaagg?tcagaattgt?ttagtgactg 7500

taattttctt?ttgcgcacta?aggaaagtgc?aaagtaactt?agagtgactg?aaacttcaca 7560

gaatagggtt?gaagattgaa?ttcataacta?tcccaaagac?ctatccattg?cactatgctt 7620

tatttaaaaa?ccacaaaacc?tgtgctgttg?atctcataaa?tagaacttgt?atttatattt 7680

attttcattt?tagtctgtct?tcttggttgc?tgttgataga?cactaaaaga?gtattagata 7740

ttatctaagt?ttgaatataa?ggctataaat?atttaataat?ttttaaaata?gtattcttgg 7800

The target practice sequence of pPHA3

taattgaatt?attcttctgt?ttaaaggcag?aagaaataat?tgaacatcat?cctgagtttt 7860

tctgtaggaa?tcagagccca?atattttgaa?acaaatgcat?aatctaagtc?aaatggaaag 7920

aaatataaaa?agtaacatta?ttacttcttg?ttttcttcag?tatttaacaa?tccttttttt 7980

tcttcccttg?cccagacaag?agtgaggttg?ctcatcggtt?taaagatttg?ggagaagaaa 8040

atttcaaagc?cttgtaagtt?aaaatattga?tgaatcaaat?ttaatgtttc?taatagtgtt 8100

gtttattatt?ctaaagtgct?tatatttcct?tgtcatcagg?gttcagattc?taaaacagtg 8160

ctgcctcgta?gagttttctg?cgttgaggaa?gatattctgt?atctgggcta?tccaataagg 8220

tagtcactgg?tcacatggct?attgagtact?tcaaatatga?caagtgcaac?tgagaaacaa 8280

aaacttaaat?tgtatttaat?tgtagttaat?ttgaatgtat?atagtcacat?gtggctaatg 8340

gctactgtat?tggacagtac?agctctggaa?cttgcttggt?ggaaaggact?ttaatatagg 8400

tttcctttgg?tggcttaccc?actaaatctt?ctttacatag?caagcattcc?tgtgcttagt 8460

tgggaatatt?taattttttt?ttttttttaa?gacagggtct?cgctctgtcg?cccaggctgg 8520

agtgcagtgg?cgcaatctcg?gctcactgca?aactccgctc?ccacaacaca?gtgacaagca 8580

tctcaggtaa?ggtaccataa?cttcgtatag?catacattat?acgaagttat?attgctagag 8640

tcagcttctg?atggaattag?aacttggcaa?aacaatactg?agaatgaagt?gtatgtggaa 8700

cagaggctgc?tgatctcgtt?cttcaggcta?tgaaactgac?acatttggaa?accacagtac 8760

ttagaaccac?aaagtgggaa?tcaagagaaa?aacaatgatc?ccacgagaga?tctatagatc 8820

tatagatcat?gagtgggagg?aatgagctgg?cccttaattt?ggttttgctt?gtttaaatta 8880

tgatatccaa?ctatgaaaca?ttatcataaa?gcaatagtaa?agagccttca?gtaaagagca 8940

ggcatttatc?taatcccacc?ccacccccac?ccccgtagct?ccaatccttc?cattcaaaat 9000

gtaggtactc?tgttctcacc?cttcttaaca?aagtatgaca?ggaaaaactt?ccattttagt 9060

ggacatcttt?attgtttaat?agatcatcaa?tttgtgcaga?cttacagcgg?atcgatcccc 9120

tcagaagaac?tcgtcaagaa?ggcgatagaa?ggcgatgcgc?tgcgaatcgg?gagcggcgat 9180

accgtaaagc?acgaggaagc?ggtcagccca?ttcgccgcca?agctcttcag?caatatcacg 9240

ggtagccaac?gctatgtcct?gatagcggtc?cgccacaccc?agccggccac?agtcgatgaa 9300

tccagaaaag?cggccatttt?ccaccatgat?attcggcaag?caggcatcgc?catgggtcac 9360

gacgagatcc?tcgccgtcgg?gcatgcgcgc?cttgagcctg?gcgaacagtt?cggctggcgc 9420

gagcccctga?tgctcttcgt?ccagatcatc?ctgatcgaca?agaccggctt?ccatccgagt 9480

The target practice sequence of pPHA3

acgtgctcgc?tcgatgcgat?gtttcgcttg?gtggtcgaat?gggcaggtag?ccggatcaag 9540

cgtatgcagc?cgccgcattg?catcagccat?gatggatact?ttctcggcag?gagcaaggtg 9600

agatgacagg?agatcctgcc?ccggcacttc?gcccaatagc?agccagtccc?ttcccgcttc 9660

agtgacaacg?tcgagcacag?ctgcgcaagg?aacgcccgtc?gtggccagcc?acgatagccg 9720

cgctgcctcg?tcctgcagtt?cattcagggc?accggacagg?tcggtcttga?caaaaagaac 9780

cgggcgcccc?tgcgctgaca?gccggaacac?ggcggcatca?gagcagccga?ttgtctgttg 9840

tgcccagtca?tagccgaata?gcctctccac?ccaagcggcc?ggagaacctg?cgtgcaatcc 9900

atcttgttca?atggccgatc?ccatattggc?tgcaggtcga?aaggcccgga?gatgaggaag 9960

aggagaacag?cgcggcagac?gtgcgctttt?gaagcgtgca?gaatgccggg?cctccggagg 10020

accttcgggc?gcccgccccg?cccctgagcc?cgcccctgag?cccgcccccg?gacccacccc 10080

ttcccagcct?ctgagcccag?aaagcgaagg?agcaaagctg?ctattggccg?ctgccccaaa 10140

ggcctacccg?cttccattgc?tcagcggtgc?tgtccatctg?cacgagacta?gtgagacgtg 10200

ctacttccat?ttgtcacgtc?ctgcacgacg?cgagctgcgg?ggcggggggg?aacttcctga 10260

ctaggggagg?agtagaaggt?ggcgcgaagg?ggccaccaaa?gaacggagcc?ggttggcgcc 10320

taccggtgga?tgtggaatgt?gtgcgaggcc?agaggccact?tgtgtagcgc?caagtgccca 10380

gcggggctgc?taaagcgcat?gctccagact?gccttgggaa?aagcgcctcc?cctacccggt 10440

agaattgacc?tgcaggggcc?ctcgactata?acttcgtata?gcatacatta?tacgaagtta 10500

tgggttcacg?ccattctcct?gcctcagcct?cccgagtagc?tgggactaca?ggcgcccgcc 10560

atcacgcccg?gctaatcttt?tgtattttta?gtagagatgg?ggtttcaccg?tgtgccagga 10620

tggtctcaat?ctcctgacat?cgtgatctgc?ccacctcggc?ctcccaaagt?gctgggatta 10680

caggagtgag?tcaccgcgcc?cggcctattt?aaatgttttt?taatctagta?aaaaatgaga 10740

aaattgtttt?tttaaaagtc?tacctaatcc?tacaggctaa?ttaaagacgt?gtgtggggat 10800

caggtgcggt?ggttcacacc?tgtaatccca?gcactttgga?aggctgatgc?aggaggattg 10860

cttgagccca?ggagtacaag?accagcctgg?gcaagtctct?ttaaaaaaaa?caaaacaaac 10920

aaacaaaaaa?attaggcatg?gtggcacatg?cctgtagtcc?tagctactta?ggaggctgac 10980

gtaggaggat?cgtttggacc?tgagaggtca?aggctacagt?gagccatgat?tgtgccactg 11040

cactccagcc?tgggtgacag?agtgagactc?tgtctcaaaa?aagaaaaagg?aaatctgtgg 11100

ggtttgtttt?agttttaagt?aattctaagg?actttaaaaa?tgcctagtct?tgacaattag 11160

The target practice sequence of pPHA3

atctatttgg?catacaattt?gcttgcttaa?tctatgtgtg?tgcatagatc?tactgacaca 11220

cgcatacata?taaacattag?ggaactacca?ttctctttgc?gtaggaagcc?acatatgcct 11280

atctaggcct?cagatcatac?ctgatatgaa?taggctttct?ggataatggt?gaagaagatg 11340

tataaaagat?agaacctata?cccatacatg?atttgttctc?tagcgtagca?acctgttaca 11400

tattaaagtt?ttattatact?acatttttct?acatcctttg?tttcagggtg?ttgattgcct 11460

ttgctcagta?tcttcagcag?tgtccatttg?aagatcatgt?aaaattagtg?aatgaagtaa 11520

ctgaatttgc?aaaaacatgt?gttgctgatg?agtcagctga?aaattgtgac?aaatcacttg 11580

taagtacatt?ctaattgtgg?agattctttc?ttctgtttga?agtaatccca?agcatttcaa 11640

aggaattttt?tttaagtttt?ctcaattatt?attaagtgtc?ctgatttgta?agaaacacta 11700

aaaagttgct?catagactga?taagccattg?tttcttttgt?gatagagatg?ctttagctat 11760

gtccacagtt?ttaaaatcat?ttctttattg?agaccaaaca?caacagtcat?ggtgtattta 11820

aatggcaatt?tgtcatttat?aaacacctct?ttttaaaatt?tgaggtttgg?tttctttttg 11880

tagaggctaa?tagggatatg?atagcatgta?tttatttatt?tatttatctt?attttattat 11940

agtaagaacc?cttaacatga?gatctaccct?gttatatttt?taagtgtaca?atccattatt 12000

gttaactacg?ggtacactgt?tgtatagctt?actcatcttg?ctgtattaaa?actttgtgcc 12060

cattgattag?taacccctcg?tttcgtcctc?ccccagccac?tggcaaccag?cattatactc 12120

tttgattcta?tgagtttgac?tactttagct?accttatata?agtggtatta?tgtactgttt 12180

atctttttat?gactgactta?tttcccttag?catagtgcat?tcaaagtcca?accatgttgt 12240

tgcctattgc?agaatttcct?tcttttcaag?gctgaataat?attccagtgc?atgtgtgtac 12300

cacattttct?ttatccatta?atttgttgat?tgatagacat?ttaggttggt?tttctacatc 12360

ttgactatca?tgaatagtgt?tgcaatgaac?acaggagagc?tactatctct?tagagatgat 12420

atcatggttt?ttatcatcag?aaaacaccca?ctgatttcta?tgctaatttt?gttacctggg 12480

tggaataata?gtacagctat?atattcctca?ttttagatat?ctttgtattt?ctacatacaa 12540

taaaaaagca?gagtacttag?tcatgttgaa?gaactttaaa?cttttagtat?ttccagatca 12600

atcttcaaaa?caaggacagg?tttatctttc?tctcaccact?caatctatat?atacctcttg 12660

tgggcaaggc?cagtttttat?cactggagcc?tttccccttt?ttattatgta?cctctccctc 12720

acagcagagt?caggacttta?actttacaca?atactatggc?tctacatatg?aaatcttaaa 12780

aatacataaa?aattaataaa?ttctgtctag?agtagtatat?tttccctggg?gttacgatta 12840

The target practice sequence of pPHA3

ctttcataat?aaaaattaga?gataaggaaa?ggactcattt?attggaaagt?gattttaggt 12900

aacatttctg?gaagaaaaat?gtctatatct?taatagtcac?ttaatatatg?atggattgtg 12960

ttactcctca?gttttcaatg?gcatatacta?aaacatggcc?ctctaaaaag?ggggcaaatg 13020

aaatgagaaa?ctctctgaat?gtttttctcc?cctaggtgaa?ttcacctgct?gcttagaagc 13080

ttattttctc?ttgatttctg?ttataatgat?tgctcttacc?ctttagtttt?aagtttcaaa 13140

ataggagtca?tataactttc?cttaaagcta?ttgactgtct?ttttgtcctg?ttttattcac 13200

catgagttat?agtgtgacag?ttaattctta?tgaaaattat?atagagatgg?ttaaatcatc 13260

agaaactgta?aacctcgatt?gggaggggaa?gcggattttt?aaatgatttc?ctgaccaagc 13320

ttaaccagta?tattaaatcc?tttgtactgt?tctttggcta?taaagaaaaa?aggtactgtc 13380

cagcaactga?aacctgcttt?cttccattta?gcataccctt?tttggagaca?aattatgcac 13440

agttgcaact?cttcgtgaaa?cctatggtga?aatggctgac?tgctgtgcaa?aacaagaacc 13500

tgagagaaat?gaatgcttct?tgcaacacaa?agatgacaac?ccaaacctcc?cccgattggt 13560

gagaccagag?gttgatgtga?tgtgcactgc?ttttcatgac?aatgaagaga?catttttgaa 13620

aaagtaagta?atcagatgtt?tatagttcaa?aattaaaaag?catggagtaa?ctccataggc 13680

caacactcta?taaaaattac?cataacaaaa?atattttcaa?cattaagact?tggaagtttt 13740

gttatgatga?ttttttaaag?aagtagtatt?tgataccaca?aaattctaca?cagcaaaaaa 13800

tatgatcaaa?gatattttga?agtttattga?aacaggatac?aatctttctg?aaaaatttaa 13860

gatagacaaa?ttatttaatg?tattacgaag?atatgtatat?atggttgtta?taattgattt 13920

cgttttagtc?agcaacatta?tattgccaaa?atttaaccat?ttatgcacac?acacacacac 13980

acacacacac?ttaacccttt?tttccacata?cttaaagaat?gacagagaca?agaccatcat 14040

gtgcaaattg?agcttaattg?gttaattaga?tatctttgga?atttggaggt?tctggggaga 14100

atgtcgatta?caattatttc?tgtaatattg?tctgctatag?aaaagtgact?gtttttcttt 14160

ttcaaaattt?agatacttat?atgaaattgc?cagaagacat?ccttactttt?atgccccgga 14220

actccttttc?tttgctaaaa?ggtataaagc?tgcttttaca?gaatgttgcc?aagctgctga 14280

taaagctgcc?tgcctgttgc?caaaggtatt?atgcaaaaga?atagaaaaaa?agagttcatt 14340

atccaacctg?attttgtcca?ttttgtggct?agatttaggg?aacctgagtg?tctgatacaa 14400

actttccgac?atggtcaaaa?aagccttcct?tttatctgtc?ttgaaaatct?ttcatctttg 14460

aaggcctaca?ctctcgtttc?ttcttttaag?atttgccaat?gatgatctgt?cagaggtaat 14520

The target practice sequence of pPHA3

cactgtgcat?gtgtttaaag?atttcaccac?tttttatggt?ggtgatcact?atagtgaaat 14580

actgaaactt?gtttgtcaaa?ttgcacagca?aggggacaca?gttcttgttt?atcttttcat 14640

gataattttt?agtagggagg?gaattcaaag?tagagaattt?tactgcatct?agatgcctga 14700

gttcatgcat?tcattccata?aatatatatt?atggaatgct?ttattttctt?ttctgaggag 14760

tttactgatg?ttggtggagg?agagactgaa?atgaattata?cacaaaattt?aaaaattagc 14820

aaaattgcag?cccctgggat?attagcgtac?tctttctctg?acttttctcc?cacttttaag 14880

gctctttttc?ctggcaatgt?ttccagttgg?tttctaacta?catagggaat?tccgctgtga 14940

ccagaatgat?cgaatgatct?ttccttttct?tagagagcaa?aatcattatt?cgctaaaggg 15000

agtacttggg?aatttaggca?taaattatgc?cttcaaaatt?taatttggca?cagtctcatc 15060

tgagcttatg?gaggggtgtt?tcatgtagaa?tttttcttct?aattttcatc?aaattattcc 15120

tttttgtagc?tcgatgaact?tcgggatgaa?gggaaggctt?cgtctgccaa?acagagactc 15180

aagtgtgcca?gtctccaaaa?atttggagaa?agagctttca?aagcatggta?aatactttta 15240

aacatagttg?gcatctttat?aacgatgtaa?atgataatgc?ttcagtgaca?aattgtacat 15300

ttttatgtat?tttgcaaagt?gctgtcaaat?acatttcttt?ggttgtctaa?caggtagaac 15360

tctaatagag?gtaaaaatca?gaatatcaat?gacaatttga?cattattttt?aatcttttct 15420

tttctaaata?gttgaataat?ttagaggacg?ctgtcctttt?tgtcctaaaa?aaagggacag 15480

atatttaagt?tctatttatt?tataaaatct?tggactctta?ttctaatggt?tcattatttt 15540

tatagagctg?taggcatggt?tctttattta?attttttaaa?gttattttta?atttttgtgg 15600

atacagagta?ggtatacata?tttacggggt?atatgagata?ttttgatata?agtatacaac 15660

atatataatc?cctttattta?attttatctt?ccccccaatg?atctaaaact?atttgcttgt 15720

ccttttatgt?cttatagtta?aattcagtca?ccaactaagt?tgaagttact?tcttattttt 15780

gcatagctcc?agctctgatc?ttcatctcat?gtttttgcct?gagcctctgt?tttcatatta 15840

cttagttggt?tctgggagca?tactttaata?gccgagtcaa?gaaaaatact?agctgccccg 15900

tcacccacac?tcctcacctg?ctagtcaaca?gcaaatcaac?acaacaggaa?ataaaatgaa 15960

aataatagac?attatgcatg?ctctctagaa?actgtcaatt?gaactgtatt?tgctcatcat 16020

tcctaccatc?tacaccacca?aaatcaacca?aatttatgaa?aaaaaaacag?ccccaacata 16080

aaattataca?cagataaaca?ggctatgatt?ggttttggga?aagaagtcac?ctttacctga 16140

tttaggcaac?tgtgaaatga?ctagagaatg?aagaaaatta?gacgtttaca?tcttgtcata 16200

The target practice sequence of pPHA3

gagtttgaag?atagtgctgg?atctttcttt?ttataagtaa?gatcaataaa?aactccctca 16260

ttctgtagaa?gttatgattt?cttttctaag?agacctttag?aagtcagaaa?aaatgtgttt 16320

caattgagaa?aaaagataac?tggagtttgt?gtagtacttc?ccagattata?aaatgctttt 16380

gtatgtatta?tctaatttaa?tcctcaaaac?ttcttcaatt?tagcatgttg?tcatgacact 16440

gcagaggctg?aagctcagag?acgctgagcc?ctctgctaac?aagtcctact?gctaacaagt 16500

gataaagcca?gagctggaag?tcacatctgg?actccaaacc?tgatgcttct?cagcctgttg 16560

ccccttttag?agttcctttt?taatttctgc?ttttatgact?tgctagattt?ctacctacca 16620

cacacactct?taaatggata?attctgccct?aaggataagt?gattaccatt?tggttcagaa 16680

ctagaactaa?tgaattttaa?aaattatttc?tgtatgtcca?ttttgaattt?tcttatgaga 16740

aatagtattt?gcctagtgtt?ttcatataaa?atatcgcatg?ataataccat?tttgattggc 16800

gattttcttt?ttagggcagt?agctcgcctg?agccagagat?ttcccaaagc?tgagtttgca 16860

gaagtttcca?agttagtgac?agatcttacc?aaagtccaca?cggaatgctg?ccatggagat 16920

ctgcttgaat?gtgctgatga?cagggtaaag?agtcgtcgat?atgctttttg?gtagcttgca 16980

tgctcaagtt?ggtagaatgg?atgcgtttgg?tatcattggt?gatagctgac?agtgggttga 17040

gattgtcttc?tgtgctttcg?tctgtcctat?cttcaatctt?tccctgccta?tggtggtggt 17100

acctttctgt?ttttaacctg?ctataaatta?ccagataaac?ccattcactg?atttgtaact 17160

cctttcagtc?atgctctaac?tgtaaatgaa?ggcttaaact?gaagtagaac?agttacaagg 17220

ttttacttgg?cagaacatct?tgcaaggtag?atgtctaaga?agattttttt?ttcttttttt 17280

aagacagagt?ttcgctcttg?tttcccaggc?tggggtgcaa?tggtgtgatc?ttggctcagc 17340

gcaacctctg?cctcctgggt?tcaagtgatt?ttcatgcctc?agcctcccaa?gtagctggga 17400

ttacaggcat?gcgccaccac?acctggctaa?ttttgtattt?ttagtagagg?cggggtttca 17460

ccatattgtc?cagactggtc?tcgaactcct?gacctcaggt?gatccacccg?ccttggcctc 17520

ccaaagtgct?gggattacag?gcatgagcca?ccttgcccag?cctaagaaga?ttttttgagg 17580

gaggtaggtg?gacttggaga?aggtcactac?ttgaagagat?ttttggaaat?gatgtatttt 17640

tcttctctat?attccttccc?ttaattaact?ctgtttgtta?gatgtgcaaa?tatttggaat 17700

gatatctctt?ttctcaaaac?ttataatatt?ttctttctcc?ctttcttcaa?gattaaactt 17760

atgggcaaat?actagaatcc?taatctctca?tggcactttc?tggaaaattt?aaggcggtta 17820

ttttatatat?gtaagcaggg?cctatgacta?tgatcttgac?tcatttttca?aaaatcttct 17880

The target practice sequence of pPHA3

atattttatt?tagttatttg?gtttcaaaag?gcctgcactt?aattttgggg?gattatttgg 17940

aaaaacagca?ttgagtttta?atgaaaaaaa?cttaaatgcc?ctaacagtag?aaacataaaa 18000

ttaataaata?actgagctga?gcacctgcta?ctgattagtc?tattttaatt?aagtgggaat 18060

gtttttgtag?tcctatctac?atctccaggt?ttaggagcaa?acagagtatg?ttcatagaag 18120

gaatatgtgt?atggtcttag?aatacaatga?acatgttctg?ccaacttaat?aaaggtctga 18180

ggagaaagtg?tagcaatgtc?aattcgtgtt?gaacaatttc?caccaactta?cttataggcg 18240

gaccttgcca?agtatatctg?tgaaaatcaa?gattcgatct?ccagtaaact?gaaggaatgc 18300

tgtgaaaaac?ctctgttgga?aaaatcccac?tgcattgccg?aagtggaaaa?tgatgagatg 18360

cctgctgact?tgccttcatt?agctgctgat?tttgttgaaa?gtaaggatgt?ttgcaaaaac 18420

tatgctgagg?caaaggatgt?cttcctgggc?atgtaagtag?ataagaaatt?attcttttat 18480

agctttggca?tgacctcaca?acttaggagg?atagcctagg?cttttctgtg?gagttgctac 18540

aatttccctg?ctgcccagaa?tgtttcttca?tccttccctt?tcccaggctt?taacaatttt 18600

tgaaatagtt?aattagttga?atacattgtc?ataaaataat?acatgttcac?ggcaaagctc 18660

aacattcctt?actccttagg?ggtatttctg?aaaatacgtc?tagaaacatt?ttgtgtatat 18720

ataaattatg?tatacttcag?tcattcattc?caagtgtatt?tcttgaacat?ctataatata 18780

tgtgtgtgac?tatgtattgc?ctgtctatct?aactaatcta?atctaatcta?gtctatctat 18840

ctaatctatg?caatgatagc?aaagaagtat?aaaaagaaat?atagagtctg?acacaggtgc 18900

tttatatttg?gtgaaaagac?cagaagttca?gtataatggc?aatatggtag?gcaactcaat 18960

tacaaaataa?atgtttacgt?attgtcagaa?gttgtggtga?taaactgcat?ttttgttgtt 19020

ggattatgat?aatgcactaa?ataatatttc?ctaaaattat?gtaccctaca?agatttcact 19080

catacagaga?agaaagagaa?tattttaaga?acatatctct?gcccatctat?ttatcagaat 19140

ccttttgaga?tgtagtttaa?atcaaacaaa?atgttaataa?aaataacaag?tatcattcat 19200

caaagacttc?atatgtgcca?agcagtgtgt?gctttgtgta?gattatgtca?tatagttctc 19260

ataatccacc?ttccgagaca?gatactattt?attttttgag?acagagtttt?actcttgttg 19320

cccaggctgg?agtgcaatgg?tgccatctcg?gctcaccaca?accttcgcct?cccaggttca 19380

agcgattctc?ctgcctcagc?ctcctgggat?tacaggcatg?caccaccatg?cctggctaat 19440

tttgtatttt?tagtagagat?ggggtttcac?catgttggtc?agactggtct?caaactcctg 19500

acctctggtg?atatgcctgc?ctcagcctcc?taaagtgctg?ggattacagg?catgagccac 19560

The target practice sequence of pPHA3

tgtgcccagc?cgacagatac?tattattatt?tccattctac?cgagaaggag?actaaggctc 19620

tgatcattta?aataagttgc?ctaaggtgat?gcagtgatat?aagtagcaga?gctaggaatt 19680

gagccttggt?aactttaact?ctggacccca?agtccttagc?tactaagctt?tactgcatgg 19740

ggtttagtca?aattaagact?tttggaatat?gagttacttt?tgagattagc?tttgtgatat 19800

tttttgtgct?catttgtcca?acaaagtcta?ttttattttc?atcttaatta?ggtttttgta 19860

tgaatatgca?agaaggcatc?ctgattactc?tgtcgtgctg?ctgctgagac?ttgccaagac 19920

atatgaaacc?actctagaga?agtgctgtgc?cgctgcagat?cctcatgaat?gctatgccaa 19980

agtggtaggt?ttattgttgg?aaaaaaatgt?agttctttga?ctgatgattc?caataatgag 20040

aaagaaaaat?aatgcaagaa?tgtaaaatga?tatacagtgc?aatttagatc?ttttcttgag 20100

atggtttcaa?ttctggaatc?ttaaacatga?aagaaaaagt?agccttagaa?tgattaacaa 20160

aatttagact?agttagaata?gaaagatctg?aatagagcaa?tctctaaaaa?attttgatct 20220

ttttttctct?ttttcacaat?cctgagaaca?aaaaaaaatt?aaatttaaat?gttaattaga 20280

agatatttaa?cttagatgta?aagtgagtta?acctgattcc?aggattaatc?aagtactaga 20340

attagtatct?tatggcaaat?tatagaacct?atccctttag?aatattttca?aatctttttg 20400

aggatgttta?ggaatagttt?tacaagaaat?taagttagga?gaggaaatct?gttctggagg 20460

atttttaggg?ttcccactag?catatgtaat?ggtttctgaa?ctattcagaa?tcagagaaaa 20520

ctcatttttc?ctgctttcaa?gaagctactg?tatgccaggc?accatgcaca?aacaatgacc 20580

aacgtaaaat?ctctcatttt?ggagagcctg?gaatctaact?ggaaaggtga?actaataata 20640

ataatatgta?caatcatagc?catcatttat?taaactttta?ttatatgcaa?ggcactgttt 20700

aatttcatta?gcttacctgg?tttacagagc?agctctatga?gatgagtgcc?atctttgccc 20760

ctattttagg?gataaggatt?ccgaaatgtg?gagatggtaa?gtaaaattgc?acaactgaag 20820

aatgagttac?atgacttggc?tcaaatactg?gtcattgaac?tccagagcct?gaatattctt 20880

aaccacttac?atgatgcaag?ctcaccaaat?aaatagttcg?aatgtattgt?gacagagcgg 20940

cattgatatt?catctattca?tgtggctttg?agtaggaaga?agaaaggata?tcattctgac 21000

cagaggggtg?aaaaacaacc?tgcatctgat?cctgaggcat?aatactatta?acacaattct 21060

tttatgtttc?agttcgatga?atttaaacct?cttgtggaag?agcctcagaa?tttaatcaaa 21120

caaaattgtg?agctttttga?gcagcttgga?gagtacaaat?tccagaatgc?gtaagtaatt 21180

tttattgact?gatttttttt?atcaatttgt?aattatttaa?gacttaatat?atgagccacc 21240

The target practice sequence of pPHA3

tagcatagaa?cttttaagaa?tgaaaataca?ttgcatattt?ctaatcactc?tttgtcaaga 21300

aagataggag?aggagagata?aaatagttga?tggggtggag?aggtctatat?ttgaatgtag 21360

tctaaaaatt?gttctcttaa?gattggaagt?atgtaggctg?ggagggtaaa?taccaaatct 21420

tggtatatca?gaactgagca?tgtcccttga?aggttaagaa?atagttaatg?ggcaaataga 21480

gcatggcaat?attttgtaga?gcagcaagta?gtaggccttg?aatagatgtc?gctcaaaaag 21540

taatatgtaa?gctgaacaca?aaaatgtaac?aaatgaattt?agatacatat?ttgaatatta 21600

aattcaggtt?gtttgggaga?tgcacctagt?ctttgatggt?taaacctttc?cctccataga 21660

agagacagag?acagaatggc?ttgctggact?aatgtcccaa?ttcaatagag?tcttatctac 21720

gaaggttaaa?aacaagaaga?gacatattat?acagtagata?tttattgtgt?ggctcataca 21780

catggtgctc?ttctgattat?ggattttaga?gataataaca?gtgaacaaga?catagtttct 21840

ttcctcgagt?agattaaagt?catacattga?cttttaatgg?tgactggcat?tcttaataca 21900

tgattattat?atattaggta?ccatgtcaga?ttaattataa?tactttacta?tttttaattt 21960

aacccttgaa?ctatccctat?tgagtcagat?atatttcctt?ccattttcta?cttgtatctt 22020

tcaagtttag?catatgctga?tacatatgaa?gctctctcca?ggttttattg?aaagaagaaa 22080

ttaataaatt?tattaatgtc?actgaattag?gcaactcact?ttcccaagat?tatgcaagtg 22140

gtacaggtgg?aactcaaagc?caagtttaac?tagttgttca?ggagaatgtt?ttctaccctc 22200

cactaaccca?ctactctgca?gatggagata?atatgatgaa?tggaacatag?caacatctta 22260

gttgattccg?gccaagtgtt?ctctgtttta?tctactatgt?tagacagttt?cttgccttgc 22320

tgaaaacaca?tgacttcttt?ttttcaggct?attagttcgt?tacaccaaga?aagtacccca 22380

agtgtcaact?ccaactcttg?tagaggtctc?aagaaaccta?ggaaaagtgg?gcagcaaatg 22440

ttgtaaacat?cctgaagcaa?aaagaatgcc?ctgtgcagaa?gactatgtga?gtctttaaaa 22500

aaatataata?aattaataat?gaaaaaattt?tacctttaga?tattgataat?gctagctttc 22560

ataagcagaa?ggaagtaatg?tgtgtgtgtg?catgtttgtg?tgcatgtgtg?tgtgcatgca 22620

cgtgtgtgta?tgtgtgatat?tggcagtcaa?ggccccgagg?atgataattt?tttttttttt 22680

tttgagacgg?agtctcgctt?tgttgtccag?gctggagtgc?agtggtgcca?tctcggctca 22740

ctgcaacctc?cgcctcccaa?gttcaagcca?ttctcctgcc?tcagcctccc?aagtagctgg 22800

gactacaggt?gcatgccacc?atgcctggct?aattttttgt?atttttagta?gaaaattttc 22860

agcttcacct?cttttgaatt?tctgctctcc?tgcctgttct?ttagctatcc?gtggtcctga 22920

The target practice sequence of pPHA3

accagttatg?tgtgttgcat?gagaaaacgc?cagtaagtga?cagagtcacc?aaatgctgca 22980

cagaatcctt?ggtgaacagg?cgaccatgct?tttcagctct?ggaagtcgat?gaaacatacg 23040

ttcccaaaga?gtttaatgct?gaaacattca?ccttccatgc?agatatatgc?acactttctg 23100

agaaggagag?acaaatcaag?aaacaaacgt?gaggagtatt?tcattactgc?atgtgtttgt 23160

agtcttgata?gcaagaactg?tcaattcaag?ctagcaactt?tttcctgaag?tagtgattat 23220

atttcttaga?ggaaagtatt?ggagtgttgc?ccttattatg?ctgataagag?tacccagaat 23280

aaaatgaata?actttttaaa?gacaaaatcc?tctgttataa?tattgctaaa?attattcaga 23340

gtaatattgt?ggattaaagc?cacaatagaa?taacatgtta?gaccatattc?agtagaaaaa 23400

gatgaacaat?taactgataa?atttgtgcac?atggcaaatt?agttaatggg?aaccatagga 23460

gaatttattt?ctagatgtaa?ataattattt?taagtttgcc?ctatggtggc?cccacacatg 23520

agacaaaccc?ccaagatgtg?acttttgaga?atgagacttg?gataaaaaac?atgtagaaat 23580

gcaagccctg?aagctcaact?ccctattgct?atcacagggg?ttataattgc?ataaaattta 23640

gctatagaaa?gttgctgtca?tctcttgtgg?gctgtaatca?tcgtctaggc?ttaagagtaa 23700

tattgcaaaa?cctgtcatgc?ccacacaaat?ctctccctgg?cattgttgtc?tttgcagatg 23760

tcagtgaaag?agaaccagca?gctcccatga?gtttggatag?ccttattttc?tatagcctcc 23820

ccactgaagg?gagcaaagtt?taagaaccaa?atataaagtt?tctcatcttt?atagatgaga 23880

aaaattttaa?ataaagtcca?agataattaa?atttttaagg?atcattttta?gctctttaat 23940

agcaataaaa?ctcaatatga?cataatatgg?cacttccaaa?atctgaataa?tatataattg 24000

caatgacata?cttcttttca?gagatttact?gaaaagaaat?ttgttgacac?tacataacgt 24060

gatgagtggt?ttatactgat?tgtttcagtt?ggtcttccca?ccaactccat?gaaagtggat 24120

tttattatcc?tcatcatgca?gatgagaata?ttgagactta?tagcggtatg?cctggcccaa 24180

gtactcagag?ttgcctggct?ccaagattta?taatcttaaa?tgatgggact?accatcctta 24240

ctctctccat?ttttctatac?gtgagtaatg?ttttttctgt?tttttttttt?tctttttcca 24300

ttcaaactca?gtgcacttgt?tgagctcgtg?aaacacaagc?ccaaggcaac?aaaagagcaa 24360

ctgaaagctg?ttatggatga?tttcgcagct?tttgtagaga?agtgctgcaa?ggctgacgat 24420

aaggagacct?gctttgccga?ggaggtacta?cagttctctt?cattttaata?tgtccagtat 24480

tcatttttgc?atgtttggtt?aggctagggc?ttagggattt?atatatcaaa?ggaggctttg 24540

tacatgtggg?acagggatct?tattttacaa?acaattgtct?tacaaaatga?ataaaacagc 24600

The target practice sequence of pPHA3

actttgtttt?tatctcctgc?tctattgtgc?catactgttg?aatgtttata?atgcatgttc 24660

tgtttccaaa?tttgtgatgc?ttatgaatat?taataggaat?atttgtaagg?cctgaaatat 24720

tttgatcatg?aaatcaaaac?attaatttat?ttaaacattt?acttgaaatg?tggtggtttg 24780

tgatttagtt?gattttatag?gctagtggga?gaatttacat?tcaaatgtct?aaatcactta 24840

aaatttccct?ttatggcctg?acagtaactt?ttttttattc?atttggggac?aactatgtcc 24900

gtgagcttcc?atccagagat?tatagtagta?aattgtaatt?aaaggatatg?atgcacgtga 24960

aatcactttg?caatcatcaa?tagcttcata?aatgttaatt?ttgtatccta?atagtaatgc 25020

taatattttc?ctaacatctg?tcatgtcttt?gtgttcaggg?taaaaaactt?gttgctgcaa 25080

gtcaagctgc?cttaggctta?taagtcgacc?tatactttat?attttttttt?tcttaaaagt 25140

agctgtgggg?agttcctgtc?gtggcgcagt?ggttaacgaa?cccgactagg?aactatgagg 25200

ttgcgggttc?ggtccctgcc?cttgctcagt?gggttaacga?tccggcattg?ccgtgagctg 25260

tggtgtaggt?tgcagacgcg?gctcggatcc?agcgttgctg?tggctctggc?gtaggccggt 25320

ggctgcagct?ccgattagac?ccctagcctg?ggaacctcca?tatgccgtgg?gagcggccca 25380

agaaatagca?aaaagacaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aagtagctgt 25440

ggaattccct?ggtggctcag?cgagttaagg?gtccagcatt?tttgctgctg?tggctctgat 25500

tactgttgtg?gggcaggttt?gatccctggc?ctaggagctt?ctgcagaccg?caggcacagc 25560

caaaggggaa?aaaaaagtaa?ttataatact?tattattgaa?agcaaagagt?gactgaccag 25620

tgccagtcac?tattctaaac?tcattatata?tattaacttc?tttaattctc?aaaatatttg 25680

cctgagttga?ataccattat?tattctcctt?ttgcaaatga?gaagacttct?cctgtggctc 25740

acatcataac?catttctcaa?atgaggaaaa?gaaggcacag?aacaaggcct?aggctaaata 25800

atttacctaa?ggaccactca?actagtgaga?ggtggggcca?agatgcaaaa?ttcaaaccag 25860

ggcttgagag?ctcaccactg?tcctttgtga?cctaatgtgg?tgcagaagag?tcatttaact 25920

aagcactggc?tcaacagtgg?gttactttag?gtagaggcta?gactaaatgc?aagtgcattt 25980

ctacatgtaa?aaacccaact?gaatatcact?gatttcacat?ggttaaccag?atagctcaag 26040

aacaaattca?tttcactgga?ggatgtgata?gttcaacctg?tgtgaacttc?tactctaagt 26100

aaataatttg?ctacagtttc?caatgtgttg?acatgctggg?ttttttgttt?gtttgtttgt 26160

ttttcggtct?ttttgccatt?tctaaggccg?ctcctgtggc?atatggaggt?gcctaggcta 26220

gggatccaat?tggagctgca?gctgctggcc?tacaccacag?ccacagcaat?gcgggatccg 26280

The target practice sequence of pPHA3

agctgtgtct?gcaacctaca?ccacagctca?cggcaacgcc?ggatccttta?acccactgag 26340

caagggcagg?gaccgaaccc?acaacctcat?ggttcctagt?tggatttgtt?aaccactgcg 26400

ccactacggg?aaccccgaga?acttcttttc?ttatgacaaa?agatcggctt?taaaatttag 26460

atgtttgaac?ataaacatgt?tttacccatt?ttggttataa?tattttttga?gaagttaaaa 26520

aaataagatg?aacttccttt?tttgaaacta?gttgtccagc?tatattttat?tttattatgt 26580

tttggccacc?tcacagcata?tggagctccc?gggccaggga?tgagatctga?gctacagtca 26640

ttcgcaacct?aagccatagc?tgtggaaatg?ctggattttt?cacccactca?gacaggctgg 26700

ggattgaacc?tgcatcccgg?ccttcccaag?acactgccaa?tcccttcatg?ccacagtagg 26760

aactccatcc?aactattttt?tagcatgaat?aattttcata?tttttcaaac?tgattgtgat 26820

gtttattcca?ctgtgaggtt?ttaaccttgc?tctttgctaa?taggataata?cttataattg 26880

tgcattcggc?caaagaacca?acttcagatc?tctgcaaatt?tgggcatttc?aaaatgtgat 26940

ttgtcttagt?cctaaatggc?aagactttct?tcctagaggt?ctaacttgga?gaagacacct 27000

tttttgtttg?taattaactt?agtgagaagt?agatgttaag?ttaatgactc?tgagggtcga 27060

tgaggaaaag?gctgaggagt?ccaatgacac?tggtgtcaaa?gagaggactt?tacaatcctt 27120

atagcccaac?agaagaattt?agccgccatg?agtctaggac?agccttaaat?tgttttcact 27180

ggtggaaatt?acagaaagac?agtctaagca?atttagcatt?catttgaata?gatttgagaa 27240

aacacacagc?attttataaa?cagtaattat?atttatcctt?ttgtttttca?gactaccctg 27300

agaataagag?aaagagaaat?gaagacctag?acttatccat?ctctttttct?tttctgttgg 27360

ttttaaacca?acaccctgtc?taaagtacac?aaatttcttt?aaatattttg?cctcttttct 27420

ctgtgctaca?attaataaaa?aaatgaaaag?aatctaattt?aattgtctat?gactgttatt 27480

tttttgaaga?tgtgttgtca?acctgataat?tttgtaggtt?ctatgaaaat?tccactattc 27540

tctctttccc?tatttcaatg?gaggacttct?agttccttct?ggattaattg?cataaaagaa 27600

acattaatac?tgccatgagt?tatggattat?gaacattcaa?agtaatattt?taacattatg 27660

ataattctga?ataaaagagt?agagcctgtg?gtataggtga?gacatataaa?acatggttta 27720

tggagttccc?ttcatggcac?agtggaaacg?aatctgacta?ggaaccatgg?aggtttcggg 27780

ttcaatccct?ggcctcactc?agtgggttaa?ggatccagtg?ttgctgtgag?ctgtggtata 27840

gtttgcaaac?ttggctcgta?tctggcattg?ctgtggcttt?ggcctaggcc?ggcagctgta 27900

gctccgattt?gacccttggc?ctgggaacca?tcatatgcag?cagaagaggc?ccaagcttaa 27960

The target practice sequence of pPHA3

tcac 27964

Claims (4)

1. three kinds have the carrier and the construction process thereof that can carry out the pig serum albumin gene target practice, can be on pig albumin gene seat with the human serum albumin positioning integration, make the transgenic pig of preparation can in serum, produce human serum albumin, and do not produce the serum albumin of pig itself, thereby supply raw materials for producing human serum albumin.

2. according to the construction process of claims 1 described targeting vector, the carrier that comprises the pig serum albumin gene group sequence construct production foreign protein that is published in sequence table that uses the clone, the homologous sequence that 5 ' and 3 ' end use of three kinds of targeting vectors in this patent is announced at this patent, before 5 ' end homologous sequence comprises ATG-and the 14272bp sequence, part is used or is all used; 3 ' end comprises the 21044bp sequence behind the ATG, and part is used or all used.

3. has 50% above homology sequence according to claims 2 described sequences.

4. the gene targeting that uses claims 2 described sequences to be used for pig is produced the carrier of foreign protein.

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