CN1694756A - Implementation of microfluidic components in a microfluidic system - Google Patents
Implementation of microfluidic components in a microfluidic system Download PDFInfo
- Publication number
- CN1694756A CN1694756A CN 03824980 CN03824980A CN1694756A CN 1694756 A CN1694756 A CN 1694756A CN 03824980 CN03824980 CN 03824980 CN 03824980 A CN03824980 A CN 03824980A CN 1694756 A CN1694756 A CN 1694756A
- Authority
- CN
- China
- Prior art keywords
- microfluidic
- substrate
- microchannel
- film
- capping module
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system.
Description
Related application
The right of priority of U.S. Provisional Patent Application No.60/409489 that the application requires to enjoy in the U.S. Patent application No.10/329018 that submitted on December 23rd, 2002, submitted on September 9th, 2002 and the U.S. Provisional Patent Application No.60/410685 that submits on September 13rd, 2002, the content of these applications is incorporated herein by reference.
Invention field
The present invention relates to a kind of microfluidic systems that is used for treat fluid sample under the microjet level.More particularly, the present invention relates to a kind of system and method that is used for realizing microfluidic function in microfluidic systems.
Background of invention
Microfluidic device and system provide chemistry, biological chemistry and biological analysis and the improved method of synthetic carried out.Microfluidic device and system allow to carry out rapid, the eurypalynous chemical operation of multistep in the little chemical analysis system of chip base.Chip base microfluidic systems generally includes traditional " microjet " element, and it especially can processing and analytical chemistry and biological sample.As a rule, the term in this area " microjet " is meant the system or the device of the network with processing node, chamber and storage tank of linking to each other by passage, wherein the typical section size of these passages be in about 1.0 microns in about 500 microns scope.In the art, the passage with these sectional dimensions is called " microchannel ".
In chemistry, biomedical, bio-science and pharmaceutical industry, carrying out in the mode of highly-parallel that a large amount of chemical operation become as reaction, separation and subsequent detection step is to press for.Synthesizing, screen and analyzing of the high yield of (biology) compound makes it possible to find economically new medicine and drug candidate, and can realize complicated medical diagnostic equipment.For the improvement of chemical operation required in using at these, importantly improve speed, strengthen reproducibility, reduce the consumption of expensive samples and reagent, and reduce the waste of material.
In the field of biotechnology especially cytology and medicine screening, need the particulate filter of high yield.Need the example of filtering particle that various types of cells is arranged, for example thrombocyte, white cell, tumour cell and protoblast etc.These particles gain a special interest in cytological field.Other particle has (greatly) molecular species, for example protein, enzyme and polynucleotide.In the field of the drug screening during new drug development, this class particle gains a special interest.
Brief summary of the invention
The invention provides a kind of being used for is integrated into microfluidic systems so that make microfluidic systems can carry out the system and method for selected microfluidic function with microfluidic components.The present invention has used the capping module that includes the microfluidic element that can carry out microfluidic function.This capping module stack is on the microfluidic substrate with microjet pipeline, so that microfluidic function is integrated in the system.
According to an aspect, the invention provides the microfiltration system in a kind of microfluidic chip, it is used for the material such as the compound separation of the closed channel system of process capillary dimensions are become different components.Filtering system of the present invention provides filtration module, and it can assemble by lower one-tenth originally, can provide time per unit to filter the accurate means of relatively large compound simultaneously.Microfiltration system is integrated into traditional membrane filtration technology in the microfluidic systems that is formed by glass, plastics or other suitable material.The filtering system of little manufacturing can comprise in the microfluidic systems that is designed for the standard of being inserted into so that filtering subsystem on the sheet to be provided.An exemplary filtering system comprises that film has been isolated the material of first flowing-path of flowing through by size discrimination by separated two flowing-paths of film.Be formed with the storage tank that communicates with flowing-path in the both sides of film.On film, connect the lid of little manufacturing, thereby defined the storage tank of film top.
According on the other hand, can adopt the closure construction that wherein is formed with electromagnetic valve components that magnetic valve is integrated in the microfluidic systems.Electromagnetic valve components comprises the mobile film that is used for optionally stopping through one or more communications ports of substrate, and the actuating assembly that is used to control film position.
Brief description
Fig. 1 has shown microfluidic systems, and it includes the closure construction that is used for microfluidic function is integrated into microfluidic systems.
Fig. 2 has shown and has been applicable to the diagnostic microfluidic chip that includes microfiltration system according to an illustrative embodiment of the invention.
Fig. 3 is the perspective cross-sectional view of the microfiltration system in according to an illustrative embodiment of the invention the chip shown in Figure 2.
Fig. 4 is the detailed view of the film on the microfiltration system shown in Figure 3.
Fig. 5 has shown the lid of little manufacturing of microfiltration system shown in Figure 3.
Fig. 6 is the top view of microfiltration system shown in Figure 3.
Fig. 7 is the top view of the diagnosing chip shown in Figure 2 before the assembling microfiltration system.
Fig. 8 is the top view of the diagnosing chip shown in Figure 2 after the assembling microfiltration system.
Fig. 9 a is the top view of the direct microfiltration system of two-port formula of the alternative according to the present invention.
Fig. 9 b is the perspective cross-sectional view of microfiltration system shown in Fig. 9 a.
Figure 10 a is the top view of the direct microfiltration system of three port type of the alternative according to the present invention.
Figure 10 b is the perspective cross-sectional view of microfiltration system shown in Figure 10 a, and wherein the lid of little manufacturing is removed.
Figure 11 has shown the magnetic valve that is attached to according to an embodiment of the invention in the microfluidic systems.
The detailed description of invention
The invention provides a kind of filtering system that is used to allow sample is carried out filtration on the sheet, purification or isolating little manufacturing.The filtering system of little manufacturing can be used in many kinds application, and include but not limited to blood separation and filtration, micro-dialysis, need little chemical analysis of filtration or dialysis subsystem and synthesize application, and other microfluidic application.To the present invention be described at an exemplary embodiment hereinafter.Those skilled in the art will appreciate that the present invention can implement in multiple different application and embodiment, and its application can not be limited in the specific embodiment as herein described particularly.
The employed term of this paper " microjet " refers to a kind ofly to be used to load and unload, handles, the system that includes at least one passage with microscale dimensions or the device of ejection and/or analysing fluid samples.
The employed term of this paper " passage " and " flow passage " refer to a kind of form or in medium and allow the path of fluid such as liquids and gases motion.Passage in the microfluidic systems preferably has and is in about 1.0 microns sectional dimensions in about 500 micrometer ranges, its more desirably be in about 25 microns in about 250 microns scope, preferably be in about 50 microns in about 150 microns scope.Those of ordinary skill in the art possesses the suitable volumes of definite flow passage and the ability of length.These scopes comprise that above-mentioned value is as the upper limit or lower limit.Flow passage can have any selected shape or setting, and their example comprises linear or nonlinear structure, and the U-shaped structure.
The employed term of this paper " microfluidic element " refers to be used for carry out the parts of the microfluidic systems of microfluidic function, it includes but not limited to: passive check valve, aggressive valve, pressure transmitter, connecting passage, membrane filtration unit, the threaded plug that is used for external connecting pipe, compression chamber, pump, and known other parts of those of ordinary skill in the art.
The employed term of this paper " film " or " strainer " refer to can be used to by size exclusion or other measure separates or the material of any proper composition of medium and size.
The employed term of this paper " substrate " refers to wherein be formed with the supporting structure that is used for transmitting the fluidic passage.
The employed term of this paper " lid " or " capping module " refer to a kind ofly have identical size with substrate or smaller slightly size, have any selected size of being formed by any selected material or shape and have the structure of microfluidic element.The capping module structure becomes to be stacked on the substrate or can communicate by letter with it, thereby completely or partially finishes fluid path.
The employed term of this paper " material " refers to be used in any material in the microjet technology, includes but not limited to compound, molecule, virus, cell, particle, bead, buffer reagent, and perhaps any other can be used on material in the microjet technology.
The employed term of this paper " microfluidic function " refers to include but not limited to any operation, function or technology that the fluid in the microfluidic systems or sample are carried out or applied: filtration, dialysis, pumping, fluid flow are regulated, control fluid flow rate etc.
The employed term of this paper " port " refers to provide the structure of fluid connection between two elements.
The employed term of this paper " pump " refers to be used for suck and discharge the fluidic device, and it can be of different sizes, and comprises microscale dimensions, is called " Micropump " here.
The present invention allows to adopt the capping module with the microfluidic element that can carry out microfluidic function that different microfluidic function is implemented in the microfluidic chip.As shown in Figure 1, be suitable for realizing that the microfluidic chip 10 of one embodiment of the invention comprises substrate 11, it has one or more flow passages that are shown as the microchannel 3 located therein.Flow passage can transmit fluid through microfluidic systems 10, so that any other suitable operation is handled, loads and unloads and/or carried out to fluid samples.Microfluidic systems 10 can comprise the flow passage 3 that is used for transmitting fluid process microfluidic systems 10 of any proper amt.
As shown in Figure 1, flow passage 3 is formed in the substrate 11, and can be via one or more communications ports 13a, and 13b links to each other with substrate surface.Be provided with capping module 15 to form the fluid path of sealing on substrate 11, this capping module comprises the microfluidic element 18 that is used to carry out microfluidic function, for example strainer, one or more valve, pressure transmitter or other parts.According to an alternative, the capping module can comprise and is used for guiding once more the flow through connecting passage of microchannel of another structure periphery of fluid.This illustrative substrate 11 comprises two communications ports 13a, 13b, each port is with the part 3a that do not link to each other of flow passage 3, and 3b links to each other with substrate surface, yet those skilled in the art will recognize that and to make amendment in size, quantity and the configuration aspects of communications ports and flow passage.
This exemplary capping module 15 can comprise the connectivity port that has a common boundary with the communications ports of substrate, and/or is used for providing between first connectivity port and second connectivity port chamber 12 or the passage of fluidic paths.Those skilled in the art will recognize that the capping module can have other structure, is not limited to embodiment shown in Figure 1.
Adopt capping module 15 just microfluidic function such as for example filtration, dialysis, pumping, flow control can be integrated in the microfluidic systems 10, do not need substrate 11 is improved significantly.The substrate that is used for transmitting fluidic conduit or passage 3 that includes any amount or setting can be transformed into functional fluidic circuits, and this is that one or more to have the capping module 15 of functional microfluidic element 18 and it is arranged on substrate be to realize on the chip by selecting.According to an exemplary embodiment, can adopt and " pick up and put (pick and place) " the surface mounting equipment and technology with the identical automatization that is used for making unicircuit and utilize various closure constructions to come to form fluidic circuits having on the substrate of microchannel.The suitable equipment of putting that picks up for example can be by Manncorp, and (Huntingdon Valley PA) makes Inc..
In order to make fluidic circuits, the passage 3 in the substrate 11 can be made by chip microfabrication.Passage or pipeline can be made like this, promptly etch hemichannel in first substrate, and bonding then and/or lamination second substrate to be sealing these hemichannels, thereby form the microchannel.More complicated if desired fluidic networks, substrate can be formed by one or more layers that contains etched channels.Can in substrate, make communications ports then, so that the microchannel is linked to each other with the outside surface of substrate.The proper technology that is used to make communications ports comprises boring, laser-induced thermal etching, powder explosion or other technology as known in the art.After having made substrate and communications ports, the capping module that will have desired function is adhered on the substrate, forms the microfluidic components in the big microjet loop.
The capping module of different quantities and size can be adhered on the substrate, form microfluidic systems so that apply various microfluidic function.The capping module can be removed and change, so that can reuse substrate.
According to an exemplary embodiment, the capping module has the sectional dimension that is between about 1 millimeter to about 5 centimetres, yet those skilled in the art will recognize that the present invention is not limited to this scope.The capping module can be formed by any suitable material, includes but not limited to plastics, glass, silicon and other material known in the art.
Fig. 2 has shown the system structure of an illustrative microfluidic diagnostic chip of can content according to the present invention making.Diagnosing chip 20 can comprise one or more microfluidic components, and it can be individually or is constructed to be permeable to promote the processing of sample with combining.For example as shown in the figure, diagnosing chip 20 comprises microfiltration system 100, and the material in its separable solution is for example isolated selected particle or other particle from cell or suspension.Diagnosing chip 20 also can comprise and one or morely is used to store and supply the storage tank 90 that sample, reagent or other compound are given system, and one or more waste liquid storage tank 91 that is used to assemble the sample waste liquid.Diagnosing chip also can comprise one or more mensuration consumptions, mixing and cultivation parts, diluted sample system on the sheet for example, and it is used to handle sample, for example carries out the mixing of specific amount of sample and reagent.For example this example system comprises hydrid component 60 and cultivates zone 61.Chip also can comprise the detector 70 that is used for analyzing from thing after the filter of microfiltration system 100.Detector 70 can utilize any suitable detection means, includes but not limited to fluorescence, electrochemical analysis, dielectrophoresis, surface plasma resonance (SPR), radio frequency, heat analysis and combination thereof.Chip 10 can adopt and be used for selective control through the valve of the fluid flow rate of passage, and one or morely is arranged on the chip or chip is outer is used for the driver element of Fluid Motion Driven By Moving through the passage 3 on the chip.Those skilled in the art will recognize that microfluidic systems is not limited to diagnosing chip shown in Figure 2,, can make amendment structure, position, quantity and the combination of various microfluidic components according to the present invention.
Fig. 3,4 and 6 has shown and has been applicable to the filter subsystem 100 that is implemented in the little manufacturing in the microfluidic systems shown in Figure 2 according to an embodiment of the invention.Fig. 5 has shown and has been used for making the capping module 15 of filtering system 100 according to an embodiment of the invention.Filter subsystem is used for for example including the sample of particle and fluidic mixture by film 110 separate substances, and collects the component that is separated afterwards.According to an exemplary embodiment, adopt filter subsystem to come washed corpuscles from blood plasma, yet those skilled in the art will recognize that the present invention also includes other application.According to other application, can adopt this filtering system to come isolated viral from cell, bead isolation from cell, and separating compound, molecule or other available film come isolating material.As shown in the figure, filter subsystem 100 is formed directly on the microfluidic chip, thereby for chip has increased strainability, and do not require and carry out significant or the expensive improvement of cost.
Filter subsystem 100 utilizes traditional membrane filter 110 to isolate two flowing-paths in substrate 11, so that the small volume controlled filter of sampling.This illustrative filtration system is four port type transversal filters, it comprises and is used for providing to filtering system the first fluid flowing-path 120 of material, for example particle and fluidic mixture, and second fluid flow path 130 that is used for accepting and send thing (being filtrate) after the filter of inherent filtration system.First fluid flowing-path 120 comprises first communications ports, and it is shown as at the first inlet 121a place and the first crossing access road 121 of filtering system.First fluid flowing-path 120 comprises second communications ports, and it is shown as first exit passageway 122, and it comprises the outlet 122a that comes from filtering chamber, is used for accepting and sending the reservation material of inherent filtration system.Second fluid flow path is included in the access road 131 that the filtering chamber of second ingress and film 110 belows intersects, and second exit passageway 132 that is used for sending thing after the filter of inherent filtration system.Second fluid flow path 130 can comprise the carrier fluid that is used for transmitting filter back thing.The source drives mixture flow is system after filtration, to realize the component separation through film.Fluid source can comprise the peristaltic pump of the syringe pump of chip exterior, little manufacturing, the syringe of little manufacturing, any suitable fluid source perhaps known in the art, for example be entitled as those fluid sources described in the U.S. Provisional Patent Application No.60/391868 (attorney docket CVZ-019-2) of " microfluidic systems and parts ", the content of this application is incorporated herein by reference.
The filtering system 100 of this exemplary little manufacturing has less floorage (less than about 1 square millimeter), has caused compact structure, lower cost simply to be made with relative.This particle separator also provides the relatively low deformation rate that has seldom or do not stop up.If necessary, the Fluid Volume that is kept can be very big, and in addition if necessary, this design can be at extra analytical procedure bi-directional scaling and repetition.
Filter subsystem of the present invention can form by the microfluidic chip that the intersection 101 that includes two flow passages 120,130 is provided.The parts that assembly technology is only made for integrated batch, it is simple relatively, and can realize low-cost under large vol.According to an exemplary embodiment, chip is formed with the groove 140 that communicates with second flowing-path 130 at intersection 101 places.First flowing-path 120 is initial separated or separately by groove 140.Adopt suitable adhesive or other suitable connection mechanism with suitable film 110 attached on the microfluidic chip, so that the covering groove is used for holding filter back thing and transmits the storage tank of filter back thing through second flowing-path 130 thereby formed below film.This film can comprise any suitable filtration membrane known in the art.
The capping module 15 of the exemplary little manufacturing of shown in Figure 4 this is fixed on the top of film 110, thereby has formed a filtering chamber 161 that communicates with first flowing-path 120.Lid 15 can adopt suitable adhesive or other suitable connection mechanism to connect.This exemplary capping module 15 comprises inlet 162 and the outlet 163 that is communicated with filtering chamber, so that first flowing-path 120 is linked to each other with filtering chamber 161, and realizes composition to be filtered flowing via the filtering chamber above the film.Perhaps, film 110 directly is fixed on the capping module 15, and the capping module is connected on the substrate, thereby filtering system is integrated on the substrate.Those skilled in the art will recognize that the capping module is not limited to this exemplary embodiment, can content according to the present invention change.
Fig. 7 has shown microfluidic systems 10, and it is included in assembling and has the passage 3 that just is formed at before the capping module 15 of film 110 wherein.Fig. 8 is the decline top view of fluidic system 10 of the capping that possesses strainability.
Composition to be filtered is incorporated into the filter subsystem from access road, and enters on the filtering chamber neutralized film 110.The component of material is separated by film 110, and less component such as plasma flow enter into storage tank 140 through film, and second flowing-path 130 of flowing through.Rest part such as hemocyte are flowed through filtering chamber and are arrived the outlet of first flowing-path 120.
According to this exemplary embodiment, the substrate of microfluidic chip can be formed by glass, plastics, silicon, quartz, pottery or any other suitable material.In the microfluidic chip of being made by glass, chip can comprise two-layer, i.e. chip and being fixed on the chip to form the lid of filter subsystem.In the microfluidic chip that is formed by plastics, these parts can be pressed in the plastic.
Shown in Fig. 9 a and 9b, according to an alternative, the micro-filtration subsystem can comprise double port type direct filtration device 180, and it comprises the film 110 that is inserted in the fluid flow path 181.As shown in the figure, double port type direct filtration device comprises the fluid flow path 181 that is formed in the microfluidic substrate, and it is divided into two part 181a, 181b.Second section 181b has formed groove 182, and film 110 sticks on the groove, has formed the filtering chamber that is used to accept to filter the back thing.The lid 15 that comprises little manufacturing of the groove 186 that has formed filtering chamber is being connected on the substrate above the film, so that link to each other with flowing-path 181.Material to be filtered is sent in the filtering chamber 186 via fluid flow path 181, and the film 110 of flowing through.Film 110 comes separate substance by capturing bigger molecule, and thing and flows out microfiltration system for further analysis, processing and collection etc. along flow through film and enter into groove 182 of fluid flow path 181 after the filter that is made of the residue molecule.
Shown in Figure 10 a and 10b, according to another embodiment, microfiltration system can comprise three port type direct filtration devices 190.This three port type direct filtration device 190 comprises and is used for two kinds of samples are input in the filtering chamber 195 two inlet flow passages 191,192, and is used for filter back thing is sent out an exit passageway 193 of strainer 190.This three port type direct filtration device comprises the lid 15 of the little manufacturing that defines filtering chamber, and the film 110 that filtering chamber and exit passageway 193 are separated.In operation, provide two kinds of samples via access road 191,192.Sample mixes in filtering chamber 195, and sample mixture is through membrane filtration, the component of the separable sample mixture of this film.Flowing through, thing is transmitted through exit passageway after the filter of film, to be used for further processing, analysis and collection etc.
The technician of film separation field can recognize, filtering system described here can be used to realize separate on all types of sheets that comprise film, it comprises by size and comes isolated molecule, perhaps from molecule, isolate bead, perhaps from macroparticle, isolate small-particle, perhaps from cell, isolate virus, perhaps be used for realizing other separation known to those of skill in the art.
According to another embodiment of the present invention, can adopt capping module 15 that magnetic valve is attached in the microfluidic systems.In Figure 11, shown and be used for the example that is contained in the magnetic valve in the closure construction that content according to the present invention realizes in microfluidic systems.As shown in the figure, electromagnetic module 150 comprises lid 15, and it has formed inner chamber 151, has been used for optionally stopping up the mobile film 154 via one or two communications ports in the substrate, and the actuating assembly 160 that is used to make film 154 deflections.According to this exemplary embodiment, actuating assembly comprises coil 162 and magnet 164.Those skilled in the art will recognize that and to use other can make the appropriate device of film deflection, comprise piezo actuator.
Those skilled in the art will recognize that the capping module is not limited to this exemplary embodiment, can adopt other element to increase other microfluidic function, as filtering and flow control additional or alternative.
Microfiltration system of the present invention can be advantageously in conjunction with the function and the scope of the conventional films technology of institute's inherent small volume dynamic flow control in the microfluidic systems that possesses little manufacturing/microstructure.The invention provides any suitable polymeric film and worthwhile the mixing of the cost efficiency of microjet network.It is simple and lower-cost that this microfiltration system is added on the microfluidic systems, and this is relatively low for the cost of microfluidic systems itself because of the additional cost that microfiltration system is assembled in the microfluidic chip.
Can include one or more above-mentioned parts in combination individually or with other parts according to microfluidic systems of the present invention.
The present invention has been described hereinbefore at an exemplary embodiment.Owing to can carry out certain change to said structure without departing from the scope of the invention, therefore comprise in the foregoing description or be shown in all foregoings in the accompanying drawing and all should be interpreted as exemplaryly, do not possess restrictive, sense.
Should be appreciated that following claim covered all of invention described here general with concrete feature, all of the scope of the invention are set out on the language and all belong to wherein.
Claims (20)
1. microfluidic systems comprises:
Be formed at first microchannel in the substrate;
First communications ports that described first microchannel is linked to each other with the surface of described substrate; With
Have the capping module of the microfluidic element that is used to carry out microfluidic function, wherein, described capping module can be stacked on the described substrate and be arranged to and be communicated with described microchannel, thereby described microfluidic function is incorporated in the described microfluidic systems.
2. system according to claim 1 is characterized in that, described system also comprises second communications ports that second microchannel is linked to each other with the surface of described substrate.
3. system according to claim 2 is characterized in that, described capping module comprises and is used for chamber that described first microchannel is linked to each other with described second microchannel.
4. system according to claim 1 is characterized in that described microfluidic element comprises film.
5. system according to claim 1 is characterized in that, described microfluidic element comprises the valve that is used to regulate through the fluid flow rate of described microchannel.
6. system according to claim 1 is characterized in that described microfluidic element comprises pressure transmitter.
7. system according to claim 1 is characterized in that described microfluidic element comprises the microjet pump.
8. system according to claim 2 is characterized in that, described capping module also comprises the film that is used for optionally blocking described communications ports, and the actuating assembly that is used to control the position of described film.
9. capping module that is used for microfluidic systems comprises:
Substrate; With
Be located at being used on the described substrate and carry out the microfluidic element of microfluidic function, wherein said capping module can be stacked on the microfluidic systems that wherein is formed with the microchannel, make described microfluidic element be arranged to be communicated with, thereby described microfluidic function is incorporated in the described microfluidic systems with described microchannel.
10. capping module according to claim 9 is characterized in that, described capping module also comprises the chamber that is formed in the described substrate, and described chamber is arranged in described capping module stack and is communicated with described microchannel fluid on described microfluidic systems the time.
11. system according to claim 9 is characterized in that, described microfluidic element comprises film.
12. system according to claim 9 is characterized in that, described microfluidic element comprises valve.
13. system according to claim 9 is characterized in that, described microfluidic element comprises pressure transmitter.
14. system according to claim 9 is characterized in that, described microfluidic element comprises the microjet pump.
15. a microfluidic systems comprises:
Be formed at the microchannel in the substrate;
The communications ports that described microchannel is linked to each other with the surface of described substrate; With
Have groove and covered described groove so that form the capping module of the film in chamber, described lid can be assemblied on the described substrate, makes described film cover described communications ports.
16. a method of making microfluidic systems comprises step:
In first substrate, etch hemichannel;
Bonding second substrate to be sealing described hemichannel, thereby forms the microchannel;
Process communications ports in one of in described first and second substrates, so that the outside surface one of in described microchannel and described first and second substrates is linked to each other; With
The closure construction that will have a microfluidic element one of is adhered in described first and second substrates.
17. a method of making fluidic circuits comprises:
Substrate is provided, and it has the microchannel that is formed at wherein, and one or more communications ports that described microchannel is linked to each other with described substrate outside surface; With
The cover element that will have microfluidic element is stacked on the described substrate to cover described communications ports.
18. a microfluidic systems comprises:
Have a plurality of substrates that are formed at microchannel wherein, wherein each microchannel comprises one or more communications ports that described microchannel is linked to each other with the surface of described substrate of being used for; With
The capping module of a plurality of microjets, each described capping module has the microfluidic element that microfluidic function is carried out in associated being used to, the capping module of one or more described microjets is used to be placed on the described substrate and with one or more microchannels fluid and is communicated with, so that described relevant microfluidic function is attached in the described system.
19. the filtering system of the little manufacturing in the microfluidic systems comprises:
Be used for separating substances is become the film of first and second components;
Be used to transmit first flowing-path of described material;
Be arranged on the described film and be communicated with to accept first storage tank of described material with described first flowing-path;
Be arranged under the described film second storage tank with first component of accepting described material;
Be used to accept second flowing-path of second component of described material; With
Be communicated with to introduce the first-class body source of mobile in the described material with the moving path of described first body.
20. the filtering system of the little manufacturing in the microfluidic systems comprises:
Substrate;
Be used for transmitting first flowing-path that is in described substrate of material;
Intersect to accept and to transmit second flowing-path of component after the filter of described material with described first flowing-path;
Be formed in the described substrate and the groove that is communicated with described second flowing-path;
Be used for described separating substances is become the film of filter back component and retained fraction, it is arranged on the described groove to form filtering chamber;
Be arranged on the described film to form the lid in retention chamber;
Be used to accept and transmit the 3rd flowing-path of the retained fraction of described material.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40948902P | 2002-09-09 | 2002-09-09 | |
| US60/409,489 | 2002-09-09 | ||
| US60/410,685 | 2002-09-13 | ||
| US10/329,018 | 2002-12-23 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200710128025XA Division CN101199917A (en) | 2002-09-09 | 2003-09-09 | Implementation of microfluidic components in a microfluidic system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1694756A true CN1694756A (en) | 2005-11-09 |
| CN1331575C CN1331575C (en) | 2007-08-15 |
Family
ID=35353397
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200710128025XA Pending CN101199917A (en) | 2002-09-09 | 2003-09-09 | Implementation of microfluidic components in a microfluidic system |
| CNB038249804A Expired - Fee Related CN1331575C (en) | 2002-09-09 | 2003-09-09 | Implementation of microfluidic components in a microfluidic system |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200710128025XA Pending CN101199917A (en) | 2002-09-09 | 2003-09-09 | Implementation of microfluidic components in a microfluidic system |
Country Status (2)
| Country | Link |
|---|---|
| CN (2) | CN101199917A (en) |
| ZA (1) | ZA200502324B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105283760A (en) * | 2013-04-30 | 2016-01-27 | 惠普发展公司，有限责任合伙企业 | Microfluidic sensing device and system |
| CN106542495A (en) * | 2016-12-12 | 2017-03-29 | 墨宝股份有限公司 | A kind of preparation method of micro-electrical-mechanical system vibration jet actuator |
| CN108745012A (en) * | 2018-06-14 | 2018-11-06 | 四川大学 | It is a kind of can modular combination miniature venturi type bubble generator |
| CN113101985A (en) * | 2019-06-26 | 2021-07-13 | 京东方科技集团股份有限公司 | Detection chip and detection system |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102008002336A1 (en) * | 2008-06-10 | 2009-12-24 | Robert Bosch Gmbh | Pinch valve and method for its production |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5922210A (en) * | 1995-06-16 | 1999-07-13 | University Of Washington | Tangential flow planar microfabricated fluid filter and method of using thereof |
| SE9502258D0 (en) * | 1995-06-21 | 1995-06-21 | Pharmacia Biotech Ab | Method for the manufacture of a membrane-containing microstructure |
| CA2312102C (en) * | 1997-12-24 | 2007-09-04 | Cepheid | Integrated fluid manipulation cartridge |
-
2003
- 2003-09-09 CN CNA200710128025XA patent/CN101199917A/en active Pending
- 2003-09-09 CN CNB038249804A patent/CN1331575C/en not_active Expired - Fee Related
- 2003-09-09 ZA ZA200502324A patent/ZA200502324B/en unknown
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105283760A (en) * | 2013-04-30 | 2016-01-27 | 惠普发展公司，有限责任合伙企业 | Microfluidic sensing device and system |
| CN106542495A (en) * | 2016-12-12 | 2017-03-29 | 墨宝股份有限公司 | A kind of preparation method of micro-electrical-mechanical system vibration jet actuator |
| CN108745012A (en) * | 2018-06-14 | 2018-11-06 | 四川大学 | It is a kind of can modular combination miniature venturi type bubble generator |
| CN108745012B (en) * | 2018-06-14 | 2021-04-20 | 四川大学 | But miniature venturi type bubble generating device of modularization combination |
| CN113101985A (en) * | 2019-06-26 | 2021-07-13 | 京东方科技集团股份有限公司 | Detection chip and detection system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101199917A (en) | 2008-06-18 |
| ZA200502324B (en) | 2006-05-31 |
| CN1331575C (en) | 2007-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6878271B2 (en) | Implementation of microfluidic components in a microfluidic system | |
| US7870964B2 (en) | Implementation of microfluidic components in a microfluidic system | |
| JP6654681B2 (en) | Single-structure biochip and process for providing process from sample introduction to result output | |
| US8323887B2 (en) | Miniaturized fluid delivery and analysis system | |
| US7186383B2 (en) | Miniaturized fluid delivery and analysis system | |
| US8309039B2 (en) | Valve structure for consistent valve operation of a miniaturized fluid delivery and analysis system | |
| CN1331575C (en) | Implementation of microfluidic components in a microfluidic system | |
| AU2019344001B2 (en) | System, device and methods of sample processing using semiconductor detection chips | |
| CN115254219B (en) | Centrifugal microfluidic detection system and detection control method thereof | |
| CN114292734B (en) | Full-flow integrated liquid drop digital PCR chip, preparation method and application | |
| US20220379307A1 (en) | System and Process for Handling a Fluid Volume and Transferring said Volume into a Microfluidic System | |
| WO2005031163A1 (en) | Implementation of microfluidic components in a microfluidic system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070815 Termination date: 20090909 |