CN1693461B - Method of producing cellulose by using saccharomycete - Google Patents
Method of producing cellulose by using saccharomycete Download PDFInfo
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- CN1693461B CN1693461B CN 200510070368 CN200510070368A CN1693461B CN 1693461 B CN1693461 B CN 1693461B CN 200510070368 CN200510070368 CN 200510070368 CN 200510070368 A CN200510070368 A CN 200510070368A CN 1693461 B CN1693461 B CN 1693461B
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- enzyme
- cellulose
- powder
- sugar
- composition
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- Enzymes And Modification Thereof (AREA)
Abstract
A process for preparing cellulase by use of yeast includes such steps as adding yeast to culture medium, culturing, centrifugal separation to obtain supernatant (enzyme liquid), depositing to obtain corse enzyme, separating and purifying.
Description
Technical field
The present invention relates to a kind of preparation method of cellulase, relate in particular to a kind of method of utilizing yeast to prepare acidic cellulase.
Background technology
Cellulase is meant energy hydrolysis β-1.4 glycosidic link, making cellulose degradation is the general name of the enzyme system of glucose, comprising β-1.4 Glycosylase, endo-type β-1.4 dextran enzyme and circumscribed-type dextranase, the cellulase of different microorganisms, its 26S Proteasome Structure and Function differs greatly, cellulase can be divided into by effect optimal pH difference: acidic cellulase (optimal pH is about 4.8), it is to study more and most widely used cellulase at present, mainly by koning trichoderma, Trichodermareesei, viride, aspergillus niger, generations such as mould.Neutral cellulase (optimal pH 6～8) is mainly mould by long stalk wood, generations such as humic bacterium, genus bacillus, and alkali cellulose enzyme (optimal pH 8～11), mainly by bacillus acidocldarius, generations such as humic bacterium.The cellulose resource source that occurring in nature exists is complicated, its structure and performance difference are bigger, and the cellulase of different sources also is not quite similar to their degradation effect, Mierocrystalline cellulose at different sources is sought the higher cellulase of degradation effect, is one of the focus in current cellulase field.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing yeast to prepare acidic cellulase.
Design of the present invention is: add barms and cultivate in substratum, the supernatant liquor that obtains through centrifugation is enzyme liquid then, and enzyme liquid can be got thick enzyme through precipitate and separate, and thick enzyme purification promptly gets the product cellulase.The composition of described substratum is Sucus Cocois, water, sugar, cellulose powder, inorganic salt.
Preparation method's technology of acidic cellulase provided by the present invention is simple, and cost is low, and the gained cellulase is to bacteria cellulose, and it is active high, good degrading effect.
Description of drawings
Fig. 1 is the process flow sheet that the present invention utilizes the method for yeast production of cellulose enzyme.
Embodiment
Below in conjunction with accompanying drawing invention is further described.
Adding the candida strain nutrient solution of 1-10% in substratum, is 50～200r/min in shaking speed, and temperature is cultivated 36～108h between 20～30 ℃, obtain bacteria culture fluid; Is 2000～4000r/min centrifugation, 10～20min with bacteria culture fluid in shaking speed, gets its supernatant liquor, is enzyme liquid; Enzyme liquid can be got thick enzyme through precipitate and separate, and thick enzyme purification promptly gets the product cellulase.The composition of described substratum is Sucus Cocois, water, sugar, cellulose powder, inorganic salt, wherein counts by weight percentage Sucus Cocois and accounts for 50～90%, and water accounts for 0～40%, and sugar accounts for 3～8%, and cellulose powder accounts for 0.1～1%, and inorganic salt account for 0.4～1%.Sugar is meant a kind of in sucrose, glucose, lactose, sorbose, wood sugar, the fructose in the composition of described substratum.Cellulose powder is meant a kind of in bacteria cellulose powder, straw powder, bagasse powder, the coconut palm palm fibre powder in the composition of described substratum.Inorganic salt are meant (NH in the composition of described substratum
4)
2SO
4, KH
2PO
4, MgSO
4, CaCl
2
Embodiment 1
Calculate by weight Sucus Cocois 50 grams, (NH
4)
2SO
44 grams, KH
2PO
41 gram, MgSO
40.5 gram, CaCl
20.3 gram, bacteria cellulose powder 0.5 gram, sucrose 2 grams as the substratum 250ml triangular flask of packing into, add 5% candiyeast (camdida) bacteria culture fluid with this, at shaking speed 150r/min, cultivate 72h for 30 ℃ and obtain bacteria culture fluid.Is 3000r/min centrifugation 10min with bacteria culture fluid in shaking speed, gets its supernatant liquor and is enzyme liquid.Enzyme liquid through precipitate and separate get final product thick enzyme, thick enzyme promptly gets the product cellulase through separation and purification.
Embodiment 2
Calculate by weight with Sucus Cocois 400 grams water 100 grams, (NH
4)
2SO
44 grams, KH
2PO
41 gram, MgSO
40.5 gram, CaCl
20.3 gram, bacteria cellulose powder 1 gram, cane sugar powder 0.5 gram, fructose 20 grams as substratum, add 5% candiyeast (camdida) bacteria culture fluid with this, at shaking speed 150r/min, cultivate 72h for 30 ℃ and obtain bacteria culture fluid.Is 3000r/min centrifugation 10min with bacteria culture fluid in shaking speed, gets its supernatant liquor and is enzyme liquid.Enzyme liquid through precipitate and separate get final product thick enzyme, thick enzyme promptly gets the product cellulase through separation and purification.
Claims (4)
1. method of utilizing yeast production of cellulose enzyme, it is characterized in that: in substratum, add 1～10% candida strain nutrient solution, in shaking speed is 50～200r/min, and temperature is cultivated 36～108h between 20～30 ℃, obtain bacteria culture fluid; Is 2000～4000r/min centrifugation, 10～20min with bacteria culture fluid in shaking speed, gets its supernatant liquor, is enzyme liquid; Enzyme liquid can be got thick enzyme through precipitate and separate, and thick enzyme purification promptly gets the product cellulase; The composition of described substratum is Sucus Cocois, water, sugar, cellulose powder, inorganic salt, wherein counts by weight percentage Sucus Cocois and accounts for 50～90%, and water accounts for 0～40%, and sugar accounts for 3～8%, and cellulose powder accounts for 0.1～1%, and inorganic salt account for 0.4～1%.
2. according to the said method of utilizing yeast production of cellulose enzyme of claim 1, it is characterized in that: sugar is meant a kind of in sucrose, glucose, lactose, sorbose, wood sugar, the fructose in the composition of described substratum.
3. according to the said method of utilizing yeast production of cellulose enzyme of claim 1, it is characterized in that: cellulose powder is meant a kind of in bacteria cellulose powder, straw powder, bagasse powder, the coconut palm palm fibre powder in the composition of described substratum.
4. according to the said method of utilizing yeast production of cellulose enzyme of claim 1, it is characterized in that: inorganic salt are meant (NH in the composition of described substratum
4)
2SO
4, KH
2PO
4, MgSO
4, CaCl
2
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510070368 CN1693461B (en) | 2005-04-30 | 2005-04-30 | Method of producing cellulose by using saccharomycete |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510070368 CN1693461B (en) | 2005-04-30 | 2005-04-30 | Method of producing cellulose by using saccharomycete |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1693456A CN1693456A (en) | 2005-11-09 |
| CN1693461B true CN1693461B (en) | 2010-04-14 |
Family
ID=35352583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510070368 Expired - Fee Related CN1693461B (en) | 2005-04-30 | 2005-04-30 | Method of producing cellulose by using saccharomycete |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1693461B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348776B (en) * | 2008-07-31 | 2011-08-17 | 中国农业科学院北京畜牧兽医研究所 | Transgenic lactobacillus secreting acidic cellulase, preparation and use thereof |
| CN102168015B (en) * | 2010-07-27 | 2013-04-17 | 孙义 | Natural probiotic culture medium and probiotic preparation |
| CN102703337B (en) * | 2012-06-08 | 2014-07-02 | 海南大学 | Yeast strain of high-yield cellulase and screening method of yeast strain |
| CN103146601B (en) * | 2013-02-21 | 2014-10-29 | 海南大学 | Bacillus spp culture medium with coconut water as main raw material |
| CN104064791B (en) * | 2014-02-26 | 2017-01-11 | 武汉科技大学 | Bioelectrocatalysis inverted-conversion reactor of microbial fuel cell, purification method of CO2 in gas and preparation method of CO2 biological synthetic fuel |
| CN112852783A (en) * | 2021-01-18 | 2021-05-28 | 临夏市哈利德清真食品有限公司 | Preparation method of enzymolysis agent for processing fruit juice beverage |
-
2005
- 2005-04-30 CN CN 200510070368 patent/CN1693461B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 宁静.碱性纤维素酶产生菌的筛选及其产酶条件.科技通报16 3.2000,16(3),216-218. |
| 宁静.碱性纤维素酶产生菌的筛选及其产酶条件.科技通报16 3.2000,16(3),216-218. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1693456A (en) | 2005-11-09 |
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