CN1680238A - Preparation and use of compounds i.e., 1,3-dihydroxy-5-alkyl benzene as inhbitor of protein-tyrosine-phosphatase 1B - Google Patents
Preparation and use of compounds i.e., 1,3-dihydroxy-5-alkyl benzene as inhbitor of protein-tyrosine-phosphatase 1B Download PDFInfo
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Abstract
Production of 1,3-dihydroxy-5-alkylamine compound with protein tyrosine phosphatase 1B inhibiting activity extracted and separated from branches and leaves of Lumnitzera racemosa Willd and its use are disclosed. R1 and R2 is H or Ac, R3 is H or CH3, n=8 or 10. It is carried out by drying and breaking branches and leaves of Lumnitzera racemosa, extracting by methyl alcohol, extracting the subsection of light petroleum, acetic ether and (n-)butanol, silica gel column and Sephadex LH-20 gel column chromatographing the part of acetic ether, separating to obtain monomer compound, analyzing by wave spectrum, determining 1,3-dihydroxy-5-alkylamine compound, acetylating and methylating reacting, and obtaining acetylate and methylate. It has PTP1B inhibiting activity and can be used for diabetes mellitus and obesity.
Description
Technical field
The present invention relates to medical technical field, specifically is a kind of separate from mangrove plant olive Lee and derivatize obtains that to have an arrestin tyrosine-phosphatase 1B (PTP1B) active 1, the preparation method and its usage of 3-dihydroxyl-5-benzene compound.This compounds can be used as inhibitor and the euglycemic agent of protein-tyrosine-phosphatase 1B, can be used for treating various diabetes, obesity and other complication that causes thus.
Technical background
Diabetes (diabetes mellitus) are one group of clinical syndromes that is caused by the h and E factor interaction, absolute or relative deficiency and target tissue cell reduce insulin sensitivity because of insulin secretion, cause a series of metabolism disorders such as sugar, albumen, fat, power and water Xie Zhi.Clinical is main common sign with the hyperglycemia, and prolonged illness can cause a plurality of system damages, be in a bad way and stress the time acute metabolism disorder such as ketoacidosis etc. can take place.Severe complications such as coronary heart disease, iron deficiency or hemorrhagic cerebrovascular disease, blind, acromelic gangrene take place in the diabetics all apparently higher than non-diabetic people.Therefore, diabetes and complication thereof have become the worldwide public health problem of serious threat human health.
Generally diabetes are divided into two classes at present, type i diabetes (insulin-dependent diabetes mellitus, IDDM) with type ii diabetes (non insulin dependent diabetes, NIDDM).It in the diabetes type ii diabetes.WHO estimates that because the mode of life of aging population, obesity, unsound diet and shortage motion, by 2025, diabetic subject's number will rise to 300,000,000 by 1.35 hundred million of nineteen ninety-five.
The type i diabetes people is owing to the HLA-D gene on the 6th pair of the short arm of a chromosome has determined genetic predisposition, to environmental factors, particularly the abnormal reaction of virus infection or chemical toxicity material incentive directly or indirectly passes through autoimmune response, cause that B cell destroys, so that insufficient insulin.Clinical characters is to cause disease urgency, many foods, diuresis, drink more, lose weight etc. symptom is more obvious, has the tendency that ketosis is poisoned takes place, and must rely on insulinize and earn a bare living.
Type ii diabetes also has very strong heredity and environmental factors, and is significant heterogeneity, and pathogenesis is various and complicated, exists than big-difference between each patient.Generally speaking can be summarized as the relative deficiency and the insulin resistant of insulin secretion.To the type ii diabetes people, especially a series of of adiposis patient studies confirm that, insulin resistant is the key factor in type ii diabetes generation, the evolution.On the basis of insulin signaling pathway, designing and developing euglycemic agent in research adipocyte and muscle cell, to improve the insulin resistant state, is the emphasis of present type ii diabetes new drug research, also is one of its main direction.
The characteristics of type ii diabetes are the opposings to insulin action of insulin sensitivity tissue such as skeletal muscle, liver, fatty tissue.Though its concrete mechanism it be unclear that, insulin signaling weakening even blocking in its conduction path must be direct factor.Regular Insulin is by combining β subunit intrinsic tyrosine kinase activity in the activated receptor born of the same parents with the outer alpha subunit of its acceptor born of the same parents, cause tyrosine residues autophosphorylation crucial in the adjustment structure territory, thereby activate the insulin receptor tyrosine kinase activity fully, insulin receptor tyrosine kinase hands on signal by its substrate of phosphorylation again.Along with the intensification of reversibility tyrosine phosphorylation understanding in the insulin action path in the pair cell, protein-tyrosine-phosphatase (PTPases) is the more importance attached in the associated protein tyrosine phosphorylation level in this path of balance.PTPases may act on a plurality of links in this path, for example with autophosphorylation activatory insulin receptor (IR) dephosphorylation, thereby reduces kinase activation; Or will be, thereby path behind the negative regulation insulin action acceptor such as the phosphorylation of causing a disease of protein-tyrosine residue in the substrate of insulin receptors such as substrate 1 (IRS-1), IRS 2 (IRS-2), Shc.The active imbalance of Tyrosylprotein kinase glucose-6-phosphate dehydrogenase may be the reason that causes the type ii diabetes insulin resistant in specific PTPases and the Regular Insulin path.Therefore, suppress its activity, strengthen and the prolongation insulin signaling, become the new way of more and more valued treatment type ii diabetes by the inhibitor of seeking selectively acting PTPases in this path.
PTPases comprises that a big nation strides (non-receptor type) enzyme in film (receptor type) and the born of the same parents, participates in a series of important vital processes of regulation and control.Though multiple PTPases has expression in the tissue of insulin sensitivity, as stride the CD45 of film and LAR-PTPase etc.; SHPTP-1, SHPTP-2, PTP1B, PTP1C etc. in the born of the same parents, but have only several PTPases may be in the Regular Insulin path acceptor or acceptor metasomite influence normal insulin action.Present research mainly concentrates on LAR-PTPase, SHPTP-2, PTP1B.
PTP1B is purified and the PTPase of definite biological characteristics the about 50KD of total length the earliest.Studies have shown that in early days can be external effectively with the insulin receptor dephosphorylation; The PTP1B microinjection that derives from people's placenta is gone in the African toad ovocyte, will reduce insulin-induced oocyte maturation and S6 peptide phosphorylation level.Find PTP1B high expression level in all insulin sensitivity tissues subsequently; Behind the method afford PTP1B antibody with osmotic shock, mouse KRC-7 liver cell DNA when insulin stimulating is synthetic significantly to raise with PI3 kinase activity level, and IR autophosphorylation level, IR kinase activity level and IRS-1 tyrosine phosphorylation level also significantly raise.There are some researches show that recently PTP1B IR direct and state of activation interacts; In experiment in vitro, also IRS-1 is shown the highest selective active; The high expression level of PTP1B can obviously reduce part inductive IR phosphorylation level in the rat fibroblast; Method with adenovirus mediated gene transfection, high expression level PTP1B in the model cell L6 myocyte of Regular Insulin target tissue skeletal muscle and hepatic tissue and Fao cell, IR that obvious inhibition is insulin-induced and the tyrosine phosphorylation of IRS-1, thereby and significantly suppress IRS-1 and the formation of PI3 kinases P85 subunit mixture and the phosphorylation level of Akt, MAPK, and synthetic also be suppressed [the Egawa K.et al.J.Biol.Chem.276 (13): 10207-10211] of insulin-induced glycogen.High expression level PTP1B in the model cell 3T3-L1 of another Regular Insulin target tissue fatty tissue cell uses the same method, insulin-induced IR, IRS-1 and the kinase whose tyrosine phosphorylation of PI3 of same obviously inhibition, P42 and P44 MAPK phosphorylation level also obviously reduce, and Akt phosphorylation level and active unaffected [Venable C.L.et al.J.Biol.Chem.275 (24): 18318-18326].The high expression level of PTP1B does not have influence to basic, medium and the insulin-induced glucose transport of maximum, to the EC of transhipment
50Insulin concentration does not have influence.These studies have shown that PTP1B can also mainly act on insulin receptor by negative regulation insulin signaling transduction path.Prior experimental evidence is from the PTP1B knock out mice.Reports such as Elchebly, the mouse growth of the PTP1B gene knockout that the method for utilization homologous recombination produces is normal, fecundity is arranged, insulin sensitivity is significantly strengthened, and relevant [the Elchebly M. of enhancing of insulin receptor and substrate 1 phosphorylation level in this enhancement and liver and the skeletal muscle, et al.Science, 283,1544-1548].Surprisingly, the mouse of PTP1B gene knockout also has resistant function to food-induced weight increase and insulin resistant.The mouse of the PTP1B gene knockout that the roughly the same method of utilizations such as Klaman produces also obtains same result, and why the mouse of finding the PTP1B gene knockout has resistant function to food-induced weight increase, be minimizing, and the quantity of adipocyte does not change owing to the adipocyte volume.The mouse analytic metabolism level of PTP1B gene knockout and overall energy consumption rising [Klaman L.D., et al.Molecular and Cellular Biology, 20 (15): 5479-5489].These experiments have convincingly demonstrated the vital role of PTP1B aspect insulin sensitivity, energy expenditure and fat stores more, thus clearer and more definite it be a potential drug action target spot of treatment type-II diabetes and obesity.
The research of PTP1B selective depressant has obtained certain progress, but be confined to some peptide class or non-peptide compounds mostly, for example based on inhibitor EEDE (F2PMP) M (Ki=7.2nM), the Glu-F2PMP-F2PMP (IC of the dephosphorylized substrate sequences Design of PTP1B
50=40nM), though these peptide inhibitors have the active and higher selectivity of stronger inhibition, they are that the fact of peptide class phosphate cpd makes it be difficult to become drug candidate compound.Recently, a series of non-peptide class non-phosphorylating compound class PTP1B inhibitor are in the news, and they have certain selectivity, the more important thing is, the some of them compound has remarkable effect to glucose and insulin level in the reduction ob/ob mice plasma.This is the first routine pharmacological direct evidence, proves that the PTP1B inhibitor has anti-diabetic activity [Malamas, M.S., et al.J.Med.Chem., 2000,43,1293-1310].These provide opportunity for we seek the non-peptide class of new small molecules organic compound as efficient, highly selective PTP1B inhibitor undoubtedly.
Olive Lee (Lumnitzera racemosa Willd) is mangrove plant, and the single-minded torrid zone or the seashore tideland, subtropics of growing in belongs to Combretum Racemosum." China book on Chinese herbal medicine " record, olive Lee cure mainly diseases such as white mouth, eczema, skin pruritus [editorial committee of State Administration of Traditional Chinese Medicine's " China's book on Chinese herbal medicine ". China's book on Chinese herbal medicine, Shanghai: Shanghai science tech publishing house, 1999,5,615].
The present invention separates to obtain 1 first from mangrove plant olive Lee; 3-dihydroxyl-5-benzene compound; and the process derivative reaction has obtained their methide and acetylate; studies show that through pharmacological testing repeatedly; this compounds has the remarkable activity of arrestin tyrosine-phosphatase 1B; by literature search, do not see that this compounds has active in this respect report.
Summary of the invention
The purpose of this invention is to provide a kind of from mangrove plant olive Lee extraction separation 1,3-dihydroxyl-5-benzene compound and prepare the method for its derivative;
Another object of the present invention provides a kind of above-mentioned 1, the purposes of 3-dihydroxyl-5-benzene compound.
Of the present invention 1,3-dihydroxyl-5-benzene compound has following chemical structural formula:
Wherein work as n=10, R
3=CH
3The time, R
1, R
2Be H, CH simultaneously
3Or Ac;
Work as n=8, R
3During=H, R
1, R
2Be H, CH simultaneously
3Or Ac.
The present invention is extraction separation 1 from mangrove plant olive Lee, the method for 3-dihydroxyl-5-benzene compound, and its step is as follows:
With olive Lee branches and leaves methyl alcohol lixiviate three times of drying and crushing, each week, merge methanol extract liquid, pressure reducing and steaming methyl alcohol gets methanol extract, methanol extract H
2O dissolving is used sherwood oil, ethyl acetate, n-butanol extraction respectively three times again, and solvent evaporated gets sherwood oil part, ethyl acetate part, propyl carbinol part and water-soluble portion respectively.Ethyl acetate is partly passed through silica gel (100-200 order) column chromatography, uses petrol ether/ethyl acetate 95: 5-50: 50 wash-outs, pass through Sephadex LH-20 gel filtration chromatography again, and use CHCl
3/ MeOH (1: 1) wash-out obtains 1,3-dihydroxyl-5-benzene compound.
1,3-dihydroxyl-5-benzene compound and aceticanhydride and pyridine reaction obtain its acetylate; 1,3-dihydroxyl-5-benzene compound and methylating reagent CH
2N
2Reaction obtains its methide.
The present invention is to gained 1, and 3-dihydroxyl-5-benzene compound has carried out protein-tyrosine-phosphatase 1B and suppressed activity experiment, shows that it has obvious inhibiting activity.
Test philosophy: utilize molecular biology method at intestinal bacteria system expression people source protein matter tyrosine-phosphatase 1B (hPTP1B) catalyst structure domain, the phosphatide key of hPTP1B recombinant protein energy hydrolysis substrate pNPP after purified, the product that obtains has very strong photoabsorption at the 410nm place, and variation that therefore can be by directly detecting 410nm place's photoabsorption is with the activity change of observation enzyme and the compound inhibition situation to enzymic activity.The survey live body of standard is as follows: 10mM Tris.Cl, pH 7.6,10mM pNPP, 2%DMSO, 100nM hPTP1B.
pNPP??????????????????????????????????????????????????410nm
Observation index: the dynamic measurement wavelength is the photoabsorption at 410nm place, and the time is 3 minutes, and the slope of its kinetic curve first order reaction is as the activity index of enzyme.
Inhibiting rate (%)=[1-Vmax (testing sample)/Vmax (blank)] * 100%
The judge of experimental result and explanation: The selection result be when compound concentrations is 10 μ g/ml to the percent inhibition of enzymic activity, suppress activity and be higher than at 50% o'clock, screening draws IC routinely
50, the IC of positive control sodium vanadate
50Be 2 μ M.
| 1,3-dihydroxyl-5-benzene compound n=10, R 3＝CH 3 | ??IC 50(uM) | 1,3-dihydroxyl-5-benzene compound n=8, R 3＝H | ?IC 50(uM) |
| ??R 1、R 2＝H ??R 1、R 2＝CH 3??R 1、R 2＝Ac | ??10.40±0.88 ??10.55±1.65 ??14.37±1.20 | ??R 1、R 2＝H ??R 1、R 2＝CH 3??R 1、R 2＝Ac | ?13.38±1.98 ?11.57±0.76 ?12.48±1.32 |
Embodiment
The present invention is further elaborated below in conjunction with embodiment, but do not limit the present invention.
NMR Bruker-DRX400 nmr determination.EI-MS measures with the Finnigan-MAT-95 mass spectrograph.Column chromatography silica gel, thin-layer silicon offset plate are Qingdao Marine Chemical Co., Ltd. and produce.Employed Sephadex LH-20 produces for E.Merk company, and reagent is Shanghai development chemical industry one factory's product.
PNPP is that Sigma company produces, and TrisCl and the DMSO worker that makes a living produces, and 96 orifice plates are that Abgene company produces, PTP1B by The National Center for Drug Screening express, partial purification, microplate reader is VERSAmax.
Embodiment one: compound 2-methyl-5-tridecyl-1,3-Benzenediol (2-methyl-5-tridecyl-1, preparation 3-benzendiol)
Olive Lee 3.0kg is with methyl alcohol lixiviate three times, each week, merge methanol extract liquid, and decompression is removed methyl alcohol, is used H again
2O dissolving is used sherwood oil, ethyl acetate, n-butanol extraction respectively three times then, merge the evaporate to dryness ethyl acetate partly medicinal extract 28g.
Silica gel (100-200 order) post on the ethyl acetate part 28g medicinal extract, with petrol ether/ethyl acetate 95: 5-50: 50 wash-outs, 500ml/ part, totally 100 parts, after detecting, thin-layer chromatography merges into 15 parts, and the 4th part is again through silica gel (200-300 order) column chromatography, with petroleum ether/ethyl ether 90: 10-50: 50 wash-outs, be divided into F41 (500mg), F42 (850mg), three parts of F43 (350mg), F41 passes through Sephadex LH-20 gel filtration chromatography again, uses CHCl
3/ MeOH (1: 1) wash-out, 5ml/ part, totally 30 parts, thin-layer chromatography detects, and developping agent petroleum ether/ethyl ether (1: 1) merges 13-18 part, gets compound 1 (25mg).
Its
1HNMR (TMS/CD
3OD) δ: 6.14 (2H, s, H-4,6), 2.39 (2H, t, J=7.24Hz, H-1 '), 1.99 (3H, s, 2-Me), 1.53 (2H, m, H-2 '), 1.26 (20H, m, H-3 '-H-12 '), 0.88 (3H, t, J=7.15 Hz, H-13 '); EIMS:m/z 306[M]
+Reference literature [Manju, M.R.Parthasarathy.Indian J.of Chemistry, 1977,15 (sect B), 1090-1093], authenticating compound 1 is 2-methyl-5-tridecyl-1, the 3-Benzenediol.(2-methyl-5-tridecyl-1，3-benzendiol)。
Embodiment two: compound 1,3-dihydroxyl-5-undecyl benzene (1, preparation 3-dihydroxy-5-undecylbenzene)
Silica gel (100-200 order) post on the ethyl acetate part 28g medicinal extract, with petrol ether/ethyl acetate 95: 5-50: 50 wash-outs, 500ml/ part, totally 100 parts, merge into 15 parts after thin-layer chromatography detects, the 5th part is passed through silica gel (200-300 order) column chromatography again, uses petrol ether/ethyl acetate 90: 10-50: 50 wash-outs, 100ml/ part, totally 90 parts.Thin-layer chromatography detects, and developping agent petroleum ether/ethyl ether (1: 1) is merged into three parts, and F51 (29-36,2.0g), F52 (40-55,500mg), F53 (65-85,200mg); F52 passes through Sephadex LH-20 gel filtration chromatography again, uses CHCl
3/ MeOH (1: 1) wash-out, 5ml/ part, totally 30 parts, thin-layer chromatography detects, developping agent petroleum ether/ethyl ether (1: 1), merge into F521 (10-19,300mg) and F522 (25-29,90mg) two parts; F522 passes through Sephadex LH-20 gel filtration chromatography again, uses the MeOH wash-out, 5ml/ part, and totally 30 parts, thin-layer chromatography detects, and developping agent petroleum ether/ethyl ether (1: 1) merges 18-20 part and gets compound 2 (20mg).
Its
1HNMR (TMS/CDCl
3) δ: 6.25 (2H, d, J=2.01Hz, H-1,3), 6.18 (1H, d, J=1.83Hz, H-2), 2.44 (2H, t, J=7.33Hz, H-1 '), 1.54 (2H, m, H-2 '), (1.25 16H, m, H-3 '-H-10 '), 0.88 (3H, t, J=6.60Hz, H-11 ');
13CNMR (TMS/CDCl
3) δ: 156.6 (C-1,3), 146.6 (C-5), (108.4 C-4,6), 100.5 (C-2), 36.2 (C-1 '), (23.0-32.2 C-2 '-10 '), 14.4 (C-11 '). reference literature [Marmor R.S.J.O.C, 1972,37 (18): 2901-2904], authenticating compound 2 is 1, and 3-dihydroxyl-5-undecyl benzene (1,3-dihydroxy-5-undecylbenzene).
Embodiment three: the preparation of compound 1 and compound 2 derivatives
(1) preparation of compound 1 and compound 2 methides
Take by weighing compound 1 and compound 2 sample 5.0mg respectively in the 10mL round-bottomed flask, adding is dissolved with CH
2N
2Diethyl ether solution 2mL, fling to ether and CH
2N
2After, methide (n=10, the R of compound 1
3=CH
3, R
1, R
2=CH
3) and methide (n=8, the R of compound 2
3=H, R
1, R
2=CH
3)
(2) preparation of compound 1 and compound 2 acetylates
Take by weighing compound 1 and compound 2 sample 5.0mg respectively in the 25mL round-bottomed flask, add anhydrous pyridine and each 1.5mL of aceticanhydride, stir 24h on magnetic stirring apparatus, pyridine and aceticanhydride are removed in decompression, get acetylate (n=10, the R of compound 1
3=CH
3, R
1, R
2=Ac) and acetylate (n=8, the R of compound 2
3=H, R
1, R
2=Ac).
Embodiment four: 1, the PTP1B of 3-dihydroxyl-5-benzene compound suppresses activity experiment
Experimental technique: the protein-tyrosine-phosphatase PTP1B that is used to screen is the gst fusion protein from expression in escherichia coli and purifying.Adopt ultraviolet substrate pNPP, observe of the active inhibition of different compounds, with the medicinal effect of preliminary assessment compound to recombinase.The product that the phosphatide of PTP1B hydrolysis substrate pNPP obtains has very strong photoabsorption at the 410nm place.Therefore the variation that can directly monitor the photoabsorption of 410nm place is with the activity change of observation enzyme and the compound inhibition situation to it.Sample faces with before being dissolved in DMSO and is made into proper concn, 3 times of dilutions, and 7 extent of dilution, three multiple holes are got 2 μ l sample solutions and are added 96 orifice plates, add 88 μ l assay mix (Assay buffer, pNPP, H
2O), add 10 μ lPTP1B again, the Vmax value is detected at the 410nm place on VERSAmax.Positive control sodium vanadate IC
50Be 2 μ M.Experimental result is as follows:
| 1,3-dihydroxyl-5-benzene compound n=10, R 3＝CH 3 | ??IC 50(uM) | 1,3-dihydroxyl-5-benzene compound n=8, R 3＝H | ?IC 50(uM) |
| ??R 1、R 2＝H ??R 1、R 2＝CH 3??R 1、R 2＝Ac | ??10.40±0.88 ??10.55±1.65 ??14.37±1.20 | ??R 1、R 2＝H ??R 1、R 2＝CH 3??R 1、R 2＝Ac | ?13.38±1.98 ?11.57±0.76 ?12.48±1.32 |
Claims (7)
1, a class formation following 1, the preparation method of 3-dihydroxyl-5-benzene compound
Wherein work as n=10, R
3=CH
3The time, R
1, R
2Be H, CH simultaneously
3Or Ac;
Work as n=8, R
3During=H, R
1, R
2Be H, CH simultaneously
3Or Ac.
It is characterized in that by extraction among mangrove plant olive Lee, separation and preparation process as follows:
A. olive Lee plant leaf is used sherwood oil, ethyl acetate and n-butanol extraction respectively with methyl alcohol lixiviate, united extraction liquid, and concentrating under reduced pressure is divided into ligroin extraction, ethyl acetate extract, n-butanol extract respectively;
B. with ethyl acetate extract process silica gel (100-200 order) column chromatography, use sherwood oil: ethyl acetate or sherwood oil: the ether gradient Xian take off;
C. component is passed through silica gel (200-300 order) column chromatography again among the b after merging, and use sherwood oil: ethyl acetate or sherwood oil: the ether gradient Xian take off;
D. with the extract of above-mentioned gained again at Sephadex LH-20 gel filtration chromatography, moving phase is chloroform: methyl alcohol (1: 1) or methyl alcohol Xian take off;
E. collect each Xian of take off the liquid concentrating under reduced pressure respectively required compound.The gained compound respectively with aceticanhydride pyridine and CH
2N
2Reaction obtains its acetylate and methide.
2, according to claim 11, the preparation method of 3-dihydroxyl-5-benzene compound is characterized in that extracting 1 among the step a, and what use during 3-dihydroxyl-5-benzene compound is methyl alcohol.
3, according to claim 11, the preparation method of 3-dihydroxyl-5-benzene compound, it is characterized in that moving phase is sherwood oil among the step b: ethyl acetate=95: 5-50: gradient Xian of 50 takes off.
4, according to claim 11, the preparation method of 3-dihydroxyl-5-benzene compound, it is characterized in that moving phase is sherwood oil among the step c: ether=90: 10-50: gradient Xian of 50 takes off.
5, according to claim 11, the preparation method of 3-dihydroxyl-5-benzene compound is characterized in that moving phase is chloroform in the steps d: methyl alcohol=1: 1 or methyl alcohol Xian take off.
6, as claimed in claim 11,3-dihydroxyl-5-benzene compound has the PTP1B of inhibition activity, uses in preparation treatment various diabetes, obesity and complication medicine thereof;
7, as claimed in claim 11,3-dihydroxyl-5-benzene compound is as euglycemic agent.
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