CN1676599A - Crystal purple salmonella culture medium - Google Patents
Crystal purple salmonella culture medium Download PDFInfo
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- CN1676599A CN1676599A CN 200510037638 CN200510037638A CN1676599A CN 1676599 A CN1676599 A CN 1676599A CN 200510037638 CN200510037638 CN 200510037638 CN 200510037638 A CN200510037638 A CN 200510037638A CN 1676599 A CN1676599 A CN 1676599A
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- salmonella
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- culture medium
- intestinal bacteria
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Abstract
This invention is 'YS salmonella culture medium' applied to 'fatal bacterial' detecting of 'medicinal microbiology' and is a solid culture medium. It is collocated by peptone, cystinol multivalent colibacillus Crystal Violet and bacillin. The peptone is the basic nutritional component, the Crystal Violet, multivalent colibacillus and bacillin can prevent the breeding of non-target bacterial, DL-cystinol promotes the growing of salmonella, and solves the problem of too many and too fast growing of non-target bacterial, and improves the testing ratio of the target bacterial and decrease the labor intensity.
Description
Technical field
The present invention relates to the substratum of " Micro biological Tests " aspect in a kind of being applied to " medical microbiology " " pathogenic bacteria (salmonella) " check.
Background technology
Before this technological invention, the check of salmonella is an ight soil of gathering patient, patient suspected or carrier, it is inoculated in Agar Platings such as SS, WS.SS agar, WS agar are the flat substratum that basic nutrition composition (peptone etc.), selectivity fungistat (toluylene red, brilliant green etc.), biochemical written instructions systems (lactose etc.), pH written instructions agent and other ancillary component with bacterium and peptizer (agar powder) are made.There are some shortcomings in these substratum: the one, and not enough to the inhibition of non-purpose bacterium, it is too many that the inoculation fecal sample is cultivated the back varied bacteria growing, both had been unfavorable for the growth of purpose bacterium, also is unfavorable for finding out the bacterium colony of purpose bacterium; The 2nd, the specific aim of selectivity fungistat is not strong, and the purpose bacterium is had strong restraining effect equally, and some purpose bacterium can not grow, and during particularly first separation and Culture, usually can not grow.
Summary of the invention
For overcoming the deficiencies in the prior art part, the object of the present invention is to provide a kind of make simple, easy to use, easily obtain result's novel culture medium.
For achieving the above object, the technical scheme that the present invention takes is: utilization intestinal bacteria and rafter acidfast bacilli (these two kinds is the maximum bacterium of quantity in the ight soil) specific phage are as the specificity fungistat of these assorted bacterium, with to the little chemicals of purpose mushroom toxin as auxiliary fungistat, the growth of control gram positive organism, add purpose bacteria growing promotor, so just can control the growth of non-purpose bacterium to a greater extent, help the growth of purpose bacterium more, improve the recall rate of purpose bacterium.Through experimental study, we have determined that the prescription of crystal purple salmonella culture medium is: peptone 10g, lactose 10g, Sodium Citrate 10g, sodium-chlor 5g, Ferric Ammonium Citrate 1.0g, DL-Gelucystine 0.5g, agar powder 16-25g, water 1000ml, 0.2% Viola crystallina aqueous solution 2.5ml, 0.4% bromothymol blue ethanolic soln 16ml, compound multivalence intestinal bacteria and citrobacter phage 50ml.
Because this substratum has the strong selectivity effect to the purpose bacterium, has obtained some beneficial effects: colibacillary inhibiting rate is reached 94.74%; Inhibiting rate to citrobacter reaches 46.43%.Recall rate to known purpose bacterium reaches 100%; To the detection of unknown sample, the recall rate of purpose bacterium is 2.42% (detect simultaneously with the SS nutrient agar, its recall rate is 0.95%).
Embodiment
Take by weighing peptone 10g, lactose 10g, Sodium Citrate 10g, sodium-chlor 5g, Ferric Ammonium Citrate 1.0g, DL-Gelucystine 0.5g, agar powder 16-25g, add water 1000ml, after heating is dissolved, transfer pH to 7.4-7.6, the Viola crystallina aqueous solution 2.5ml of adding 0.2%, add 0.4% bromothymol blue ethanolic soln 16ml, seal, boil, when waiting to be chilled to 40-50 ℃, add compound multivalence intestinal bacteria and citrobacter phage 50ml, shake up, pour plate is made the crystal purple salmonella plate culture medium.
Claims (1)
1. " YS salmonella substratum " is to use for reference SS, development such as substratum such as WS, do not contain Viola crystallina in original substratum, intestinal bacteria and citrobacter phage, compositions such as DL Gelucystine, contain the toluylene red strong excessively to the salmonella inhibition, the brilliant green composition that waits, this invention has increased Viola crystallina in original substratum, intestinal bacteria and citrobacter phage etc. are at the selectivity fungistat of non-salmonella, and salmonella growth stimulant, deducted toluylene red, brilliant green, its prescription and compound method are: take by weighing peptone 10g, lactose 10g, Sodium Citrate 10g, sodium-chlor 5g, Ferric Ammonium Citrate 1.0g, DL-Gelucystine 0.5g, agar powder 16-25g, add water 1000ml, after heating is dissolved, transfer pH to 7.4-7.6, the Viola crystallina aqueous solution 2.5ml of adding 0.2%, add 0.4% bromothymol blue ethanolic soln 16ml, seal, boil, when waiting to be chilled to 40-50 ℃, add compound multivalence intestinal bacteria and citrobacter phage 50ml, shake up, pour plate is made the YS plate culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510037638 CN1676599A (en) | 2005-01-07 | 2005-01-07 | Crystal purple salmonella culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510037638 CN1676599A (en) | 2005-01-07 | 2005-01-07 | Crystal purple salmonella culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1676599A true CN1676599A (en) | 2005-10-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510037638 Pending CN1676599A (en) | 2005-01-07 | 2005-01-07 | Crystal purple salmonella culture medium |
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| CN (1) | CN1676599A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102605039A (en) * | 2012-03-27 | 2012-07-25 | 绍兴天恒生物科技有限公司 | Salmonella culture medium containing crystal violet and production process of the culture medium |
| CN104195216A (en) * | 2014-09-09 | 2014-12-10 | 青岛润鑫伟业科贸有限公司 | KF streptococcus agar medium and application thereof |
| CN106282298A (en) * | 2015-05-13 | 2017-01-04 | 北京良润生物科技有限公司 | A kind of preparation method of selective medium |
-
2005
- 2005-01-07 CN CN 200510037638 patent/CN1676599A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102605039A (en) * | 2012-03-27 | 2012-07-25 | 绍兴天恒生物科技有限公司 | Salmonella culture medium containing crystal violet and production process of the culture medium |
| CN104195216A (en) * | 2014-09-09 | 2014-12-10 | 青岛润鑫伟业科贸有限公司 | KF streptococcus agar medium and application thereof |
| CN106282298A (en) * | 2015-05-13 | 2017-01-04 | 北京良润生物科技有限公司 | A kind of preparation method of selective medium |
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