CN1669625A - Process for preparing protein contamination resistant lecithin-polyethersulfone blended ultrafiltration membrane - Google Patents
Process for preparing protein contamination resistant lecithin-polyethersulfone blended ultrafiltration membrane Download PDFInfo
- Publication number
- CN1669625A CN1669625A CN 200510013005 CN200510013005A CN1669625A CN 1669625 A CN1669625 A CN 1669625A CN 200510013005 CN200510013005 CN 200510013005 CN 200510013005 A CN200510013005 A CN 200510013005A CN 1669625 A CN1669625 A CN 1669625A
- Authority
- CN
- China
- Prior art keywords
- lecithin
- film
- polyethersulfone
- ultrafiltration membrane
- polyether sulfone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention discloses a method for preparing the lecithin-polyethersulfone blending hyperfiltration membrane which can resist the protein pollution. The procedures are: a) drying the polyethersulfone in a certain temperature, and drying the lecithin in the low temperature, b) dissolving the polyethersulfone to the N,N-dimethyl formamide, adding the carbowax to produce holes and mixing them evenly, c) adding certain quantity of lecithin to the solution and mixing evenly, and getting the casting film liquid, d) stewing and debubbling the prepared liquid, scraping the glass plate by the razor blade, setting it in the air for some time, then putting it to the water and solidifying to the film, e) immersing the film in the pure water for some time, uncovering and getting the lecithin-polyethersulfone blending hyperfiltration membrane.
Description
Technical field
The present invention relates to a kind of preparation method of lecithin-polyethersulfone blended ultrafiltration membrane of anti-protein-contamination, belong to the technology of preparing of anti-protein-contamination film.
Background technology
The separation and purification of biological products is the key issues in the biotechnology industry.Because the main component of most biological products is a protein, so the core of biological products separation and purification is the separation and purification of protein.
That the isolation and purification method of protein molecule mainly comprises is centrifugal, precipitation, chromatography, ultrafiltration etc.Purification process such as gradient ultracentrifugation and precipitation can't satisfy the requirement of protein mass preparation, and also there are some problems in its security.Chromatography method specificity height, but the separation capacity is low, and separating medium needs frequent regeneration.By contrast, the milipore filter isolation technics has tangible technical advantage: ultra-filtration process does not have phase transformation, can carry out under normal temperature and low pressure, helps keeping the physiologically active of protein; Treating capacity is big, is fit to the recovery of trace protein in the weak solution and concentrating of low concentration protein; Milipore filter is the even non-individual body of being made by high molecular polymer, in use can not introduce impurity, can guarantee the security of ultra-filtration process; The ultra-filtration process energy consumption is low, and equipment volume is little, compact conformation, and technological process is simple, is easy to continuous operation and automation, also is easy to amplify.
Poly (ether-sulfone) ultrafiltration membrane is used widely with advantage such as its good mechanical property, materialization stability height, price economy.But the hydrophobic property that poly (ether sulfone) film is stronger makes the ability of anti-protein-contamination relatively poor, and protein causes separative efficiency to descend easily in the absorption of film surface.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of lecithin-polyethersulfone blended ultrafiltration membrane of anti-protein-contamination.Can be used to contain the separation of protein system effectively with the milipore filter of the method preparation, its antifouling property obviously is better than poly (ether sulfone) film.
The present invention is achieved through the following technical solutions, and a kind of preparation method of lecithin-polyethersulfone blended ultrafiltration membrane of anti-protein-contamination is characterized in that comprising following process:
(1) reagent preliminary treatment: polyether sulfone was descended dry 12～24 hours at 110～150 ℃, and lecithin was-50～-60 ℃ of following freeze dryings 1～4 hour;
(2) preparation of casting solution: polyether sulfone is dissolved in N under 60～80 ℃, in the dinethylformamide, be made into mass concentration and be 10～20% solution, the polyethylene glycol that adds the molecular weight 2000～3000 that is equivalent to polyether sulfone quality 40～90%, mix, add the lecithin that is equivalent to polyether sulfone quality 5～20%, mix;
(3) preparation of film: a certain amount of casting solution is poured on the glass plate,, puts into the water-bath freezing film with scraper striking film forming;
(4) film is soaked 24～36 hours in pure water after, film is taken off from glass plate, obtain the lecithin-polyethersulfone milipore filter.
The invention has the advantages that: the preparation method is simple, cost is low, and the hydrophily of the blended ultrafiltration membrane behind the adding lecithin obviously increases, and the absorption of proteins amount is significantly reduced, contamination resistance increases substantially, and protein solution is had good separating effect.
The specific embodiment
Embodiment one, the preparation of lecithin-polyethersulfone blended ultrafiltration membrane (film 1):
Polyether sulfone was descended dry 12 hours at 130 ℃, and lecithin was-50 ℃ of following freeze dryings 1 hour.Take by weighing polyether sulfone, N in 1: 3.6: 0.8 ratio, the polyethylene glycol of dinethylformamide and molecular weight 2000, mixing, stirring and dissolving under 60 ℃.All after the dissolving, stir the lecithin that adding down is equivalent to polyether sulfone quality 8.3%, mix.Casting solution is poured on the glass plate, and the striking film forming is soaked in the water, and film-forming after 24 hours, is taken off, obtains lecithin-polyethersulfone blended ultrafiltration membrane (film 1).
Prepared lecithin-polyethersulfone blended ultrafiltration membrane pore size distribution and even aperture distribution, lecithin is even in the film surface distributed, good hydrophilic property.
Embodiment two, the preparation of lecithin-polyethersulfone blended ultrafiltration membrane (film 2):
Polyether sulfone was descended dry 12 hours at 130 ℃, and lecithin was-50 ℃ of following freeze dryings 1 hour.Take by weighing polyether sulfone, N in 1: 3.5: 0.9 ratio, the polyethylene glycol of dinethylformamide and molecular weight 2500, mixing, stirring and dissolving under 60 ℃.All after the dissolving, stir the lecithin that adding down is equivalent to polyether sulfone quality 16.6%, mix.Casting solution is poured on the glass plate, and the striking film forming is soaked in the water, and film-forming after 24 hours, is taken off, obtains lecithin-polyethersulfone blended ultrafiltration membrane (film 2).
Prepared lecithin-polyethersulfone blended ultrafiltration membrane pore size distribution and even aperture distribution, lecithin is even in the film surface distributed, good hydrophilic property.
Embodiment three, the preparation of lecithin-polyethersulfone blended ultrafiltration membrane (film 3):
Polyether sulfone was descended dry 12 hours at 130 ℃, and lecithin was-50 ℃ of following freeze dryings 1 hour.Take by weighing polyether sulfone, N in 1: 5.1: 0.5 ratio, the polyethylene glycol of dinethylformamide and molecular weight 3000, mixing, stirring and dissolving under 60 ℃.All after the dissolving, stir the lecithin that adding down is equivalent to polyether sulfone quality 8.3%, mix.Casting solution is poured on the glass plate, and the striking film forming is soaked in the water, and film-forming after 24 hours, is taken off, obtains lecithin-polyethersulfone blended ultrafiltration membrane (film 3).
Prepared lecithin-polyethersulfone blended ultrafiltration membrane pore size distribution and even aperture distribution, lecithin is even in the film surface distributed, good hydrophilic property.
Comparative Examples one, the preparation of poly (ether-sulfone) ultrafiltration membrane (film 4):
Polyether sulfone is following dry 12 hours at 130 ℃.Take by weighing polyether sulfone, N in 1: 3.6: 0.8 ratio, the polyethylene glycol of dinethylformamide and molecular weight 2000, mixing, stirring and dissolving under 60 ℃.Casting solution is poured on the glass plate, and the striking film forming is soaked in the water, and film-forming after 24 hours, is taken off, obtains poly (ether-sulfone) ultrafiltration membrane (film 4).
Prepared poly (ether-sulfone) ultrafiltration membrane pore size distribution and even aperture distribution.
Comparative Examples two, the preparation of lecithin-polyethersulfone blended ultrafiltration membrane (film 5):
Polyether sulfone was descended dry 12 hours at 130 ℃, and lecithin was-50 ℃ of following freeze dryings 1 hour.Take by weighing polyether sulfone and N in 1: 4.5 ratio, dinethylformamide, mixing, stirring and dissolving under 60 ℃.All after the dissolving, stir the lecithin that adding down is equivalent to polyether sulfone quality 7%, mix.Casting solution is poured on the glass plate, and the striking film forming is soaked in the water, and film-forming after 24 hours, is taken off, obtains lecithin-polyethersulfone blended ultrafiltration membrane (film 5).
Prepared lecithin-polyethersulfone blended ultrafiltration membrane pore-creating character is poor, and hole and pore-size distribution are inhomogeneous.
Comparative Examples three, the preparation of poly (ether-sulfone) ultrafiltration membrane (film 6):
Polyether sulfone is following dry 12 hours at 130 ℃.Take by weighing polyether sulfone and N in 1: 3.7 ratio, dinethylformamide, mixing, stirring and dissolving under 60 ℃.Casting solution is poured on the glass plate, and the striking film forming is soaked in the water, and film-forming after 24 hours, is taken off, obtains poly (ether-sulfone) ultrafiltration membrane (film 6).
Prepared poly (ether-sulfone) ultrafiltration membrane pore-creating character is poor, and the fenestra number is few, and the aperture is little.
Table 1 has been listed the pure water flux of above-mentioned milipore filter (film 1, film 2, film 3, film 4, film 5 and film 6) and to the separating property of bovine serum albumin(BSA) (BSA) solution of 1g/L and to the adsorbance of BSA.
Table 1
The initial flux flux of film pure water flux BSA rejection attenuation rate BSA adsorbance
(L/ (m
2H)) (%) (L/ (m
2H)) (after 30 minutes, %) (μ g/cm
2)
Film 1 190 99 124 15 52
Film 2 175 98 114 12 34
Film 3 145 95 90 20 61
Film 4 205 99 120 40 92
Film 5 40 95 10 10 73
Film 6 10 100 2 50 110
Claims (1)
1, a kind of preparation method of lecithin-polyethersulfone blended ultrafiltration membrane of anti-protein-contamination is characterized in that comprising following process:
(1) reagent preliminary treatment: polyether sulfone was descended dry 12～24 hours at 110～150 ℃, and lecithin was-50～-60 ℃ of following freeze dryings 1～4 hour;
(2) preparation of casting solution: polyether sulfone is dissolved in N under 60～80 ℃, in the dinethylformamide, be made into mass concentration and be 10～20% solution, the polyethylene glycol that adds the molecular weight 2000～3000 that is equivalent to polyether sulfone quality 40～90%, mix, add the lecithin that is equivalent to polyether sulfone quality 5～20%, mix;
(3) preparation of film: a certain amount of casting solution is poured on the glass plate,, puts into the water-bath freezing film with scraper striking film forming;
(4) film is soaked 24～36 hours in pure water after, take off from glass plate, obtain the lecithin-polyethersulfone milipore filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510013005 CN1278764C (en) | 2005-01-06 | 2005-01-06 | Process for preparing protein contamination resistant lecithin-polyethersulfone blended ultrafiltration membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510013005 CN1278764C (en) | 2005-01-06 | 2005-01-06 | Process for preparing protein contamination resistant lecithin-polyethersulfone blended ultrafiltration membrane |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1669625A true CN1669625A (en) | 2005-09-21 |
| CN1278764C CN1278764C (en) | 2006-10-11 |
Family
ID=35041192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510013005 Expired - Fee Related CN1278764C (en) | 2005-01-06 | 2005-01-06 | Process for preparing protein contamination resistant lecithin-polyethersulfone blended ultrafiltration membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1278764C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100435922C (en) * | 2006-10-23 | 2008-11-26 | 天津大学 | Method for preparing a branched block polymer ultrafiltration membrane of polyethersulfone for resisting protein pollution |
| CN101862505A (en) * | 2010-07-15 | 2010-10-20 | 四川大学 | Self-electroosmosis hydrogel adhesive film |
| CN104888629A (en) * | 2015-05-20 | 2015-09-09 | 苏州市贝克生物科技有限公司 | Polysulfone/phosphatide hemodialysis membrane and preparation method thereof |
-
2005
- 2005-01-06 CN CN 200510013005 patent/CN1278764C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100435922C (en) * | 2006-10-23 | 2008-11-26 | 天津大学 | Method for preparing a branched block polymer ultrafiltration membrane of polyethersulfone for resisting protein pollution |
| CN101862505A (en) * | 2010-07-15 | 2010-10-20 | 四川大学 | Self-electroosmosis hydrogel adhesive film |
| CN101862505B (en) * | 2010-07-15 | 2014-07-30 | 四川大学 | Self-electroosmosis hydrogel adhesive film |
| CN104888629A (en) * | 2015-05-20 | 2015-09-09 | 苏州市贝克生物科技有限公司 | Polysulfone/phosphatide hemodialysis membrane and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1278764C (en) | 2006-10-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104607068B (en) | Bio-adhesion-resistant porous separation membrane as well as preparation method and application thereof | |
| CN1195577C (en) | Filter membranes for physiologically active substances | |
| CN103464006B (en) | Preparation of PDMS/PVDF (polydimethylsiloxane/polyvinylidene fluoride) blended microporous film | |
| JPH06166116A (en) | Porous polymer structure and production of said structure by thermally-induced phase separation | |
| CN103055726B (en) | Preparation method of low-pressure high-flux nanofiltration membrane | |
| CN105214511A (en) | A kind of Nano Silver/Graphene/Kynoar hybrid membranes and preparation method thereof | |
| CN110479109B (en) | Preparation method of polyvinylidene fluoride mixed matrix membrane with high flux and strong pollution resistance | |
| CN102430343A (en) | Preparation method of flat polyvinylidene fluoride micro-filtration membrane | |
| CN1748847A (en) | Process for preparing structure symmetric polyether sulfone hydrophilic porous film | |
| CN103394295A (en) | Hydrophilic PVDF (Polyvinylidene Fluoride) composite ultrafiltration membrane and preparation method thereof | |
| CN100435920C (en) | Method for preparing ultrafiltration film of poly-ether-sulfone and anti-protein-contamination type | |
| CN101073750A (en) | Method for producing density and gradient sectional porous ultrafiltration membrane | |
| CN1669625A (en) | Process for preparing protein contamination resistant lecithin-polyethersulfone blended ultrafiltration membrane | |
| CN104857858A (en) | Anti-polluting membrane material preparation method | |
| CN102512997B (en) | Hydrophilic polyethersulfone with cardo alloy ultrafiltration membrane and preparation method thereof | |
| CN1673271A (en) | The pH sensitive film with polyvinylidene fluoride/polyurethane mixture film as base film and its prepn process | |
| Hasheminasab et al. | High-performance hemodialysis membrane: Influence of polyethylene glycol and polyvinylpyrrolidone in the polyethersulfone membrane | |
| CN114471199B (en) | Polyether sulfone membrane with separation/adsorption dual functions and preparation method thereof | |
| CN110743395A (en) | Efficient antifouling hydrophilic polyethersulfone ultrafiltration membrane and preparation method thereof | |
| CN107486025B (en) | Preparation method of modified activated carbon fiber composite polyether sulfone ultrafiltration membrane, ultrafiltration membrane obtained by preparation method and application of ultrafiltration membrane | |
| CN101850217A (en) | Method for preparing skin-free homogeneous structural polyvinylidene fluoride transfer film | |
| CN101003003A (en) | Method for preparing anti-protein-contamination type acrylonitrile-sulfonamide polymer ultrafitration film | |
| CN109433031B (en) | Polyether sulfone ultrafiltration membrane and application thereof in field of hemodialysis | |
| CN106422787A (en) | Membrane-method integrated technology for traditional Chinese medicine deep processing production | |
| CN113244901A (en) | Adsorption resin-polymer porous membrane and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |