CN1660154A - Extractive and its application in medicine - Google Patents
Extractive and its application in medicine Download PDFInfo
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- CN1660154A CN1660154A CN 200410006049 CN200410006049A CN1660154A CN 1660154 A CN1660154 A CN 1660154A CN 200410006049 CN200410006049 CN 200410006049 CN 200410006049 A CN200410006049 A CN 200410006049A CN 1660154 A CN1660154 A CN 1660154A
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- haematitum
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- 239000003814 drug Substances 0.000 title claims description 9
- 229930003944 flavone Natural products 0.000 claims description 22
- 235000011949 flavones Nutrition 0.000 claims description 22
- 150000002213 flavones Chemical class 0.000 claims description 18
- 241000628997 Flos Species 0.000 claims description 13
- 210000000582 semen Anatomy 0.000 claims description 13
- 239000008194 pharmaceutical composition Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000003937 drug carrier Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 abstract 1
- 235000017491 Bambusa tulda Nutrition 0.000 abstract 1
- 241001330002 Bambuseae Species 0.000 abstract 1
- 241000132446 Inula Species 0.000 abstract 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 abstract 1
- 239000011425 bamboo Substances 0.000 abstract 1
- 229910052595 hematite Inorganic materials 0.000 abstract 1
- 239000011019 hematite Substances 0.000 abstract 1
- LIKBJVNGSGBSGK-UHFFFAOYSA-N iron(3+);oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[Fe+3].[Fe+3] LIKBJVNGSGBSGK-UHFFFAOYSA-N 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 238000012360 testing method Methods 0.000 description 13
- 241000699670 Mus sp. Species 0.000 description 11
- 241001465754 Metazoa Species 0.000 description 9
- 241000700159 Rattus Species 0.000 description 8
- 230000009182 swimming Effects 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 230000001430 anti-depressive effect Effects 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 4
- 241000581650 Ivesia Species 0.000 description 4
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 4
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 4
- 150000002212 flavone derivatives Chemical class 0.000 description 4
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 4
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 4
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 4
- 235000005493 rutin Nutrition 0.000 description 4
- 229960004555 rutoside Drugs 0.000 description 4
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 4
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 description 3
- LDCYZAJDBXYCGN-VIFPVBQESA-N 5-hydroxy-L-tryptophan Chemical compound C1=C(O)C=C2C(C[C@H](N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-VIFPVBQESA-N 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical compound CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000003208 petroleum Substances 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 239000000935 antidepressant agent Substances 0.000 description 2
- 229940005513 antidepressants Drugs 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229960004801 imipramine Drugs 0.000 description 2
- BCGWQEUPMDMJNV-UHFFFAOYSA-N imipramine Chemical compound C1CC2=CC=CC=C2N(CCCN(C)C)C2=CC=CC=C21 BCGWQEUPMDMJNV-UHFFFAOYSA-N 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 239000012567 medical material Substances 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 1
- YEDFEBOUHSBQBT-UHFFFAOYSA-N 2,3-dihydroflavon-3-ol Chemical compound O1C2=CC=CC=C2C(=O)C(O)C1C1=CC=CC=C1 YEDFEBOUHSBQBT-UHFFFAOYSA-N 0.000 description 1
- HCYAFALTSJYZDH-UHFFFAOYSA-N Desimpramine Chemical compound C1CC2=CC=CC=C2N(CCCNC)C2=CC=CC=C21 HCYAFALTSJYZDH-UHFFFAOYSA-N 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- FOGVNFMUZXDMTR-UHFFFAOYSA-N [Mg].Cl Chemical compound [Mg].Cl FOGVNFMUZXDMTR-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 229960003914 desipramine Drugs 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003939 flavanonol Natural products 0.000 description 1
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 150000007946 flavonol Chemical class 0.000 description 1
- 235000011957 flavonols Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 229940124531 pharmaceutical excipient Drugs 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 238000012205 qualitative assay Methods 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
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- 210000003462 vein Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
An extract for preventing and treating depression is extracted from 4 Chinese-medicinal materials including inula flower, hematite, bamboo leaf, etc.
Description
Invention field
The present invention relates to the extract that obtained by Flos Inulae, Dai Heshi, Folium Bambusae and Semen Sojae Preparatum, it contains 50-90 weight % total flavones, and in the purposes of anti-depression aspect.
Background technology
Extract of the present invention is by Flos Inulae, Haematitum, and Folium Bambusae and Semen Sojae Preparatum obtain, and wherein contained component Flos Inulae, Haematitum, Folium Bambusae and Semen Sojae Preparatum are the nontoxic Chinese crude drugs of pharmacopeia collection.Do not see above-mentioned four kinds of components up till now and contain of the report of the extract of 50-90 weight % total flavones at anti-depression aspect.
Summary of the invention
The inventor has now found that the extract of forming and contain 50-90 weight % total flavones by Flos Inulae, Haematitum, Folium Bambusae and Semen Sojae Preparatum has beat all antidepressant effect, the present invention is based on above-mentioned discovery and now finishes.
Therefore, the present invention relates to a kind of extract, it is a Flos Inulae, Haematitum, the extract of Folium Bambusae and Semen Sojae Preparatum and contain the total flavones of 50-90 weight %.
The invention further relates to and be used for antidepressant product or pharmaceutical composition, it contains Flos Inulae, and Haematitum, the extract of Folium Bambusae and Semen Sojae Preparatum and pharmaceutical carrier, wherein said extract contain 50-90 weight % total flavones.
The invention still further relates to by Flos Inulae, the extract that 50-90 weight % total flavones is formed and contained to Haematitum, Folium Bambusae and Semen Sojae Preparatum is used for antidepressant product or medicine purposes in preparation.
According to the present invention, extract of the present invention is by water, C
1-4Alkylol or their mixture extract Flos Inulae, Haematitum, and the mixture of Folium Bambusae and Semen Sojae Preparatum obtains.
According to the present invention, term C
1-4Alkylol is said methanol, ethanol, propanol, isopropyl alcohol, butanols, isobutanol or the tert-butyl alcohol for example.
According to the present invention, carrier is pharmaceutical field pharmaceutical excipient or a pharmaceutic adjuvant commonly used in the pharmaceutical composition of the present invention, as medical additive, and diluent or disintegrating agent etc.
According to the present invention, product described in the present invention is meant not the product as medicine, as functional food or health promoting product.
According to the present invention, total flavones can the qualitative and quantitative assay by following method in the extract of the present invention:
Qualitative
Adopt hydrochloric acid-magnesium powder reduction reaction: the sample that takes a morsel is dissolved in the hot methanol, adds after a little magnesium powder jolting drips concentrated hydrochloric acid, and the bubble end of rise takes on a red color.Contain flavone, flavonol or flavanonol in the show sample.
Assay
With the rutin is standard substance, and concrete grammar is as follows:
1. the configuration of rutin titer: accurately take by weighing the rutin standard substance 0.0752g of dry constant weight, with 30% dissolve with ethanol and be settled to 250ml, shake up to such an extent that concentration is the titer of 0.3008g/L.
2. the mensuration of working curve: get above-mentioned rutin standard solution 0.4,0.8,1.6,2.4,3.2ml respectively in 5 10ml volumetric flasks, be supplemented to 5ml with 30% ethanol, add 0.28ml 5% sodium nitrite, shake up, behind the placement 5min, add 0.28ml 10% aluminum nitrate, add 2ml 1mol/L NaOH behind the 6min, mixing, with 30% ethanol dilution to scale, in the colorimetric determination of 510nm place, reagent is blank reference behind the 10min.
3. flavones content is measured: take by weighing the extract 0.1000g that contains total flavones, with 30% dissolve with ethanol solution and be settled to 100ml, shake up to such an extent that concentration is the solution of 1.000g/L, pipette 1ml solution in the 10ml volumetric flask, by top 2 methods operation, measure absorbance, calculate flavones content with working curve gained normal equation.
According to the present invention, pharmaceutical composition of the present invention or extract can be by oral or parenteral route administrations.The dosage form that is suitable for oral administration has been said for example: tablet, capsule, solution, suspension, granule or pill etc.Be suitable for parenteral route such as vein, dosage form subcutaneous or intramuscular administration has been said injection for example.
The following examples are used for further specifying the present invention, but it does not mean that any limitation of the invention.
Embodiment 1
Flos Inulae, Haematitum, the extractive of general flavone preparation of Folium Bambusae and Semen Sojae Preparatum mixture
Preparation method
Flos Inulae 2000g, Haematitum 2000g, Folium Bambusae 1000g, Semen Sojae Preparatum 1000g, add 70% alcohol reflux 3 times (V/W=6: 1), filtration, concentrating under reduced pressure, lyophilization obtains extract 784g.Water is dispersed into suspension solution (weight ratio of extract and water is 1: 1.5), petroleum ether extraction 4 times (volume ratio of petroleum ether and aaerosol solution is 1: 1) discards petroleum ether layer, and the water layer water further disperses (medical material: water (1: 1)), centrifugal, discard precipitation; Water layer adopts two column volumes of AB-8 macroporous resin (applied sample amount of every 4L resin is equivalent to the 3kg medical material) washing, discard water elution liquid, 70% ethanol elution to eluent is faint yellow, 70% ethanol elution concentrating under reduced pressure oven dry (50 ℃), obtain containing the extract 133g of total flavones, productive rate is 2.22%.Record with top described total flavones assay method and/or colorimetry that general flavone content is 76.03 weight % in the embodiment 1 prepared extract.
Embodiment 2
The antidepressant effect evaluation of embodiment 1 extract
Experiment material
Laboratory animal: male kunming mouse, body weight 18-22g is available from the court's Experimental Animal Center; Male SPF level CD-1 mice is available from Beijing Vital River Experimental Animals Technology Co., Ltd..Male SPF level Wistar rat, body weight 160-180g is available from Beijing Vital River Experimental Animals Technology Co., Ltd..All raise Experimental Animal Center in the court.
Given the test agent: embodiment 1 extract, Desipramine (DIM, SIGMA company), imipramine (IMI, SIGMA company).
Experimental apparatus: glass cylinder, self-control mouse tail suspension laboratory observation case, stopwatch etc.
Experimental technique and result
The mice medication
50 of mices are divided into five groups at random, are respectively blank group, DIM group, the large, medium and small dosage group of embodiment 1 extract, 10 every group (or 12).Test after the once acute administration, wherein DIM group or IMI treated animal lumbar injection were tested after 0.5 hour, and other four groups equal gastric infusions are tested (the blank group is given the clothes distilled water) after 1 hour.
The rat medication
40 of rats are divided into five groups at random, are respectively blank group, DIM group, the large, medium and small dosage group of embodiment 1 extract, 8 every group.Test after the once acute administration, wherein DIM treated animal lumbar injection was tested after 0.5 hour, and other four groups equal gastric infusions are tested (the blank group is given the clothes distilled water) after 1 hour.
The mice forced swimming test
According to the method that Porsolt sets up, animal is put into high 20cm, diameter 12cm, the graduated cylinder of depth of water 10cm was observed 6 minutes, write down the back 4 minutes dead time.The results are shown in Table 1.
The mouse tail suspension test
Improve this chamber of method according to foundation such as Lucien Steru, with clip that mice tail end 20mm place is fixing, makes it be the state of hanging by the feet, observed 6 minutes, writes down the back 4 minutes dead time.The results are shown in Table 2.
The rat forced swimming test
Method according to Porsolt foundation.Rat is placed in the glass jar (diameter 18cm, high 40cm) of the tap water that fills, depth of water 23cm, 28 ℃ of water temperatures, swimming after 15 minutes to take out places the infrared ray electric heating stokehold, dries after with the moisture wiping on the animal health with dried cloth.After the administration 1 hour, once more rat is put into the water vat of above-mentioned equal conditions, the dead time of cumulative observation rat in 5 minutes.The positive control drug imipramine is at preceding 30 minutes lumbar injections of experiment.The results are shown in Table 3.
Mice 5-HTP gets rid of the head test
After the administration after 1 hour (wherein positive control drug IMI30 minute), lumbar injection 5-HTP120mg/kg, mice gets rid of a number of times in the opening entry 20min at once.The results are shown in Table 4.
Date processing: all data represent that with X ± SD group difference is checked with t.
Table 1 embodiment 1 extract is to the influence of experiment dead time of mice forced swimming
| Group | Dosage | Number of animals (only) | Non-swimming time (s) |
| Embodiment 1 extract is little in big embodiment 1 extract of blank group DIM group embodiment 1 extract | ? ????30mg/kg ????200mg/kg ????100mg/kg ????50mg/kg | ????10 ????10 ????10 ????10 ????10 | ????95.6±31.8 ????29.4±25.3** ????61.0±25.1* ????49.4±37.8** ????69.5±28.2 |
Annotate: compare * P＜0.05 * * P＜0.01 with the blank group
Table 2 embodiment 1 extract is to the mouse tail suspension influence of experiment dead time
| Group | Dosage | Number of animals (only) | The outstanding tail dead time (s) |
| Embodiment 1 extract is little in big embodiment 1 extract of blank group DIM group embodiment 1 extract | ? ??30mg/kg ??200mg/kg ??100mg/kg ??50mg/kg | ????10 ????10 ????10 ????10 ????10 | ????93.9±47.2 ????27.8±29.9** ????51.0±37.8* ????54.0±33.7* ????44.4±27.2** |
Annotate: compare * P＜0.05 * * P＜0.01 with the blank group
Table 3 embodiment 1 extract is to the influence of rat forced swimming test dead time
| Group | Dosage | Number of animals (only) | Non-swimming time (s) |
| Embodiment 1 extract is little in big embodiment 1 extract of blank group DIM group embodiment 1 extract | ? ??30mg/kg ??200mg/kg ??100mg/kg ??50mg/kg | ????8 ????8 ????8 ????8 ????8 | ????106.3±37.4 ????48.6±39.8* ????65.1±36.1* ????61.3±35.8* ????73.3±27.6 |
Annotate: compare * P＜0.05 with the blank group
Table 4 embodiment 1 extract gets rid of the influence of head test to mice 5-HTP
| Group | Dosage | Number of animals (only) | Get rid of a number of times |
| Big embodiment 1 extract of blank group IM group embodiment 1 extract is little | ? ?30mg/kg ?100mg/kg ?25mg/kg | ????12 ????12 ????12 ????12 | ????6.7±4.5 ????21.0±11.7** ????14.9±13.5* ????18.4±9.9** |
Annotate: compare * P＜0.05 * * P＜0.01 with the blank group
Conclusion
Studies show that the extractive of general flavone of embodiment 1 can significantly shorten the dead time of mice forced swimming test, mouse tail suspension test, rat forced swimming test, significantly increase a number of times that gets rid of of mice 5-HTP test.Show that it has antidepressant effect.
Claims (6)
1. Flos Inulae, Haematitum, the extract of Folium Bambusae and Semen Sojae Preparatum, it contains 50-90 weight % total flavones.
2. product or pharmaceutical composition, it comprises by Flos Inulae, Haematitum, extract that Folium Bambusae and Semen Sojae Preparatum obtain and pharmaceutical carrier, wherein said extract contains the total flavones of 50-90 weight %.
3. by Flos Inulae, Haematitum, the extract that Folium Bambusae and Semen Sojae Preparatum obtain is used for the product or the medicine purposes of extract in preparation, and wherein said extract contains 50-90 weight % total flavones.
4. the extract of claim 1, it contains 76.03 weight % total flavones.
5. the product of claim 2 or pharmaceutical composition, wherein said extract contains 76.03 weight % total flavones.
6. the product of claim 3 or pharmaceutical composition, wherein said extract contains 76.03 weight % total flavones.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100060494A CN100335110C (en) | 2004-02-27 | 2004-02-27 | Extractive and its application in medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100060494A CN100335110C (en) | 2004-02-27 | 2004-02-27 | Extractive and its application in medicine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1660154A true CN1660154A (en) | 2005-08-31 |
| CN100335110C CN100335110C (en) | 2007-09-05 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101531904B (en) * | 2009-05-05 | 2012-06-20 | 国际竹藤网络中心 | Bamboo leaves extract, preparing method and purpose thereof |
| CN102552374A (en) * | 2010-12-07 | 2012-07-11 | 北京联合大学生物化学工程学院 | Fermented soybean total flavonoids dripping pills and preparation method thereof |
| CN101780224B (en) * | 2009-01-16 | 2012-11-07 | 中国人民解放军军事医学科学院毒物药物研究所 | New application of composition containing inula flower |
| CN112807389A (en) * | 2021-01-22 | 2021-05-18 | 中国人民解放军军事科学院军事医学研究院 | Anti-fatigue pharmaceutical application of traditional Chinese medicine composition containing inula flower |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1194745C (en) * | 2002-12-31 | 2005-03-30 | 刘殿池 | Medicinal composition for high examination syndromes |
-
2004
- 2004-02-27 CN CNB2004100060494A patent/CN100335110C/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101780224B (en) * | 2009-01-16 | 2012-11-07 | 中国人民解放军军事医学科学院毒物药物研究所 | New application of composition containing inula flower |
| CN101531904B (en) * | 2009-05-05 | 2012-06-20 | 国际竹藤网络中心 | Bamboo leaves extract, preparing method and purpose thereof |
| CN102552374A (en) * | 2010-12-07 | 2012-07-11 | 北京联合大学生物化学工程学院 | Fermented soybean total flavonoids dripping pills and preparation method thereof |
| CN112807389A (en) * | 2021-01-22 | 2021-05-18 | 中国人民解放军军事科学院军事医学研究院 | Anti-fatigue pharmaceutical application of traditional Chinese medicine composition containing inula flower |
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| CN100335110C (en) | 2007-09-05 |
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