CN1657634A - Process for preparing active lactoferritin - Google Patents
Process for preparing active lactoferritin Download PDFInfo
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- CN1657634A CN1657634A CN 200410004604 CN200410004604A CN1657634A CN 1657634 A CN1657634 A CN 1657634A CN 200410004604 CN200410004604 CN 200410004604 CN 200410004604 A CN200410004604 A CN 200410004604A CN 1657634 A CN1657634 A CN 1657634A
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- lactoferrin
- acetic acid
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Abstract
A process for preparing the active lactoferritin used for food to be adsorbed easily includes such steps as dissolving lactoferritin powder in acetic acid, regulating pH value, membrane filter for removing Fe, and enzymolyzing by pepsin.
Description
Technical field
The relevant a kind of manufacture method that activates lactoferrin of the present invention refers in particular to and a kind ofly can effectively remove iron part contained in the lactoferrin, makes biological activity remarkable, is more conducive to the edible activation lactoferrin manufacture method that absorbs of human body.
Background technology
Mammals must be drunk dairy products just can make the life growth continue, the cow's milk the inside is slowly understood in dairy science research has many kinds to differ from the specific proteins and the special digestion form of other food, the absorption of general food, protein must resolve into amino acid and just can absorb, but the cow's milk protein texture ratio is more special, and the number chain amino acid of joint (victory peptide) can directly be absorbed in the human body.After so-called specific proteins is meant that milk-protein is resolved into inferior little protein by ferment, had the similar atypical hormone of special physiological function, as bactericidal properties, immunity, antioxygenation, make the growth of bone sprout cell, brain cell is grown up, neural system and recycle system normal development ... Deng, more than all separated evaluation of various victory peptide component confirm out, and be applied to medical treatment, heath food and infant formula additive, some composition such as beta lactoglobulin can stop oily matter absorption in the intestines, its resolvent small protein can suppress low density cholesterol in liver synthetic, so the cow's milk protein characteristic is that other food is unexistent, decompose its function of competence exertion but these function albumen are essential through ferment, the nearest 10 years beginnings of the research of these function small protein matter are much accounted of, and what wherein be expected most is lactoferrin.
Lactoferrin and DNA associativity are very strong in the cow's milk, and its associativity is relevant with dna sequence dna, and be favourable in addition with the research of de-iron-lactoferrin (apo-lactoferrin) discussion to the tumour cell inhibition; The lactoferrin effect of transcribing (transcription) that when handling DNA play; RNA produces beneficial effect (Syngistic stimulation) relatively; as the mammalian cell IL-1 β factor (IL-1 β gene); lactoferrin has provide protection to normal dna sequence dna cell; therefore in chemotherapy, add lactoferrin; but the normal cell eubolism also reduces side effect, and the mouse test-results confirms to stop tumour cell to shift or diffusion.Lactoferrin molecular weight about 78; 000; interior iron content; multiple function victory peptide (bioactive peptides) is hidden in the inside; this function composition is activated after ferment decomposes; common people have eaten lactoferrin can drive anti-all kinds of diseases and ailments; differ from microbiotic and antiinflammation is arranged; can make white cell improve the phagolysis strengthening immunity; and containing oxidation-resistance, hypertension and analgesic effect (effect of opoid narcoticness) or anti-adenovirus, mainly is DNA metabolism and the DNA that suppresses tool virus in the normal cell in the protective.
Yet, though above-mentioned lactoferrin can reach the expection effect that human body is had many benefits, but in implementing to use, reality finds, because this lactoferrin contains iron part, and be unfavorable for the active stability of function, make for the bad user of digestive function or young child when edible, can't bring into play the effect of the composition of useful physical function in the lactoferrin.
Summary of the invention
In sum, the present invention adjusts pH value by the lactoferrin powder is dissolved in acetic acid, and the filtering membrane that utilizes molecular weight 10,000 following particles to pass through has been finished iron removal, and by pepsic decomposition, has solved the problem that prior art exists.
The object of the present invention is to provide a kind of method of making the activation lactoferrin,, adjust the pH value by the lactoferrin powder is dissolved in acetic acid, membrane filtration, remove the iron in the lactoferrin, made this apo-lactoferrin more help the active stability of function, thereby the absorption of human body of being more convenient for.
A kind of method of making the activation lactoferrin provided by the invention, this method comprises the following steps:
Dissolving: the lactoferrin powder is dissolved in 25% acetic acid with 5% concentration, adjusts pH to 2.25;
De-iron: the lactoferrin powder aqueous acetic acid that will dissolve and adjust after the pH value carries out membrane filtration, and described filtering membrane tolerable molecular weight 10,000 following particles pass through, and obtain apo-lactoferrin;
Decompose: the apo-lactoferrin of gained is added 0.5% stomach en-(800unit/mg),, transfer pH to 2.25, add 0.5% stomach en-again and placed 1 hour with acetic acid in 35 ℃ of placements 1 hour;
Dry: the apo-lactoferrin liquid after will decomposing is through concentrating also lyophilize to moisture content below 4%.
The apo-lactoferrin that obtains through this method more helps the active stability of function, thereby the absorption of human body of being more convenient for.
The method of above-mentioned manufacturing activation lactoferrin, comprising the following step:
Dissolving: the lactoferrin powder is dissolved in 25% acetic acid with 5% concentration, adjusts pH to 2.25;
De-iron: will dissolve and adjust lactoferrin powder aqueous acetic acid after the pH value withstand voltage tubing string of packing into, at the bottom of this tubing string filtering membrane is arranged, this film tolerable molecular weight 10,000 following particle passes through, the tubing string top is injected with the acetate solution of pH2.25, stir with the pressurization of nitrogen tube and with bar magnet, increase nitrogen pressure gradually so that iron ion flows out, acetic acid and iron ion flow out from the tubing string below, stop when lactoferrin liquid is transformed into pale by pink in the tubing string, finish the operating process of de-iron, promptly got apo-lactoferrin;
Decompose: add 0.5% stomach en-(800unit/mg) in the gained apo-lactoferrin in 35 ℃ of placements 1 hour, transfer pH to 2.25, add 0.5% stomach en-again and placed 1 hour with acetic acid;
Dry: the apo-lactoferrin liquid after will decomposing is behind concentrated original volume 1/3rd, and lyophilize gets final product to moisture content below 4%.
This apo-lactoferrin can be worn into powder as edible additive or capsule etc.
The method of above-mentioned manufacturing activation lactoferrin can also comprise the following steps:
Dissolving: the lactoferrin powder is dissolved in 25% acetic acid with 5% concentration, adjusts pH to 2.25;
De-iron: will dissolve and adjust lactoferrin powder aqueous acetic acid after the pH value and pack in the dialysis membrane, this dialysis membrane specification tolerable molecular weight 10,000 following particle passes through, outer liquid stirs with 2M acetic acid and with bar magnet in 4 ℃ of refrigerator chambers, every day, secondary was changed outer liquid, dialysed altogether three, so that iron ion is given, thereby finish the de-iron program, promptly get apo-lactoferrin;
Decompose: the apo-lactoferrin of gained is added 0.5% stomach en-(800unit/mg),, transfer pH to 2.25, add 0.5% stomach en-and placed 1 hour with acetic acid in 35 ℃ of placements 1 hour;
Dry: the apo-lactoferrin liquid after will decomposing is behind concentrated original volume 1/3rd, and lyophilize is to moisture content below 4%, and abrasive dust gets final product.
This apo-lactoferrin can be worn into powder and be used as edible additive or make capsule etc.
The present invention adjusts the pH value by the lactoferrin powder is dissolved in acetic acid, utilizes membrane filtration to remove molecular weight 10,000 following particle has been finished iron removal, and by pepsic decomposition, obtained helping the active stability of function, thereby the apo-lactoferrin of the absorption of human body of being more convenient for.
Description of drawings
Fig. 1: schema of the present invention.
Embodiment
Describe the present invention in detail below in conjunction with specific embodiment, but do not limit practical range of the present invention.
At first, see also shown in Figure 1ly, activation lactoferrin manufacture method of the present invention is:
(1) dissolving: the lactoferrin powder is dissolved in 25% acetic acid with 5% concentration, adjusts pH to 2.25.
(2) de-iron: will dissolve and adjust lactoferrin powder aqueous acetic acid behind the pH withstand voltage tubing string of packing into, filtering membrane (ultrafiltration membrance ultrafilter membrane) is arranged at the bottom of the tubing string, the specification of film is only allowed molecular weight 10, particle below 000 can pass through, the tubing string top is injected with the pH2.25 acetate solution, stir with the pressurization of nitrogen tube and with bar magnet, improve nitrogen pressure gradually and then slowly flow out acetic acid and iron ion under the tubing string, lactoferrin liquid is transformed into pale by pink in the tubing string, then finish the de-iron program, the separated apo-lactoferrin (apo-lactoferrin) that promptly gets of iron part in the lactoferrin;
Maybe will dissolve and adjust lactoferrin powder aqueous acetic acid after the pH value packs in the dialysis membrane tripping device, the film size is only allowed molecular weight 10,000 following particle passes through, outer liquid stirs with 8 liters of 2M acetic acid and with bar magnet in 4 ℃ of refrigerator chambers, every day, secondary was changed outer liquid, dialysed altogether three, and then finished the de-iron program, the separated apo-lactoferrin (apo-lactoferrin) that promptly gets of iron part in the lactoferrin.
(3) decompose: this apo-lactoferrin is added 0.5% stomach en-(pepsin:800unit/mg) in 35 ℃ of placements reaction in 1 hour, transfer pH to 2.25 to add 0.5% stomach en-again with acetic acid again and placed 1 hour.
(4) drying: directly lyophilize (moisture content is 4% below) after being concentrated into original volume 1/3rd, it is interior or make the capsule use for the bottle of packing into it can be worn into powder.
By the above, the present invention is owing to removed the iron in the lactoferrin, and it is active stable to make that this lactoferrin is more conducive to function, thereby the human body of being more convenient for is edible absorbs.
More than described the preferred embodiments of the present invention, so it is not in order to limit the present invention.Those skilled in the art can not depart from the improvement and the variation of category of the present invention and spirit to embodiment disclosed herein.
Claims (3)
1, a kind of method of making the activation lactoferrin comprises the following steps:
Dissolving: the lactoferrin powder is dissolved in 25% acetic acid with 5% concentration, adjusts pH to 2.25;
De-iron: the lactoferrin powder aqueous acetic acid that will dissolve and adjust after the pH value carries out membrane filtration, and described filtering membrane tolerable molecular weight 10,000 following particles pass through, and obtain apo-lactoferrin;
Decompose: the apo-lactoferrin of gained is added 0.5% stomach en-(800unit/mg),, transfer pH to 2.25, add 0.5% stomach en-again and placed 1 hour with acetic acid in 35 ℃ of placements 1 hour;
Dry: the apo-lactoferrin liquid after will decomposing is through concentrating also lyophilize to moisture content below 4%.
2, the method for manufacturing activation lactoferrin as claimed in claim 1 comprises the following steps:
Dissolving: the lactoferrin powder is dissolved in 25% acetic acid with 5% concentration, adjusts pH to 2.25;
De-iron: will dissolve and adjust lactoferrin powder aqueous acetic acid after the pH value withstand voltage tubing string of packing into, at the bottom of this tubing string filtering membrane is arranged, this film tolerable molecular weight 10,000 following particle passes through, and the tubing string top is injected with the acetate solution of pH2.25, stirs with the pressurization of nitrogen tube and with bar magnet, increase nitrogen pressure gradually, acetic acid and iron ion flow out from the tubing string below, stop when lactoferrin liquid is transformed into pale by pink in the tubing string, promptly get apo-lactoferrin;
Decompose: the gained apo-lactoferrin is added 0.5% stomach en-in 35 ℃ of placements 1 hour, transfer pH to 2.25, add 0.5% stomach en-again and placed 1 hour with acetic acid;
Dry: the apo-lactoferrin liquid after will decomposing is behind concentrated original volume 1/3rd, and lyophilize is to moisture content below 4%, and abrasive dust gets final product.
3, the method for manufacturing activation lactoferrin as claimed in claim 1 comprises the following steps:
Dissolving: the lactoferrin powder is dissolved in 25% acetic acid with 5% concentration, adjusts pH to 2.25;
De-iron: will dissolve and adjust lactoferrin powder aqueous acetic acid behind the pH value and pack in the dialysis membrane, film size tolerable molecular weight 10,000 following particle passes through, outer liquid stirs with 2M acetic acid and with bar magnet in 4 ℃ of refrigerator chambers, every day, secondary was changed outer liquid, dialysed the separated apo-lactoferrin that promptly gets of iron part in the lactoferrin altogether three;
Decompose: the gained apo-lactoferrin is added 0.5% stomach en-,, transfer PH to 2.25, add 0.5% stomach en-and placed 1 hour with acetic acid in 35 ℃ of placements 1 hour;
Dry: the apo-lactoferrin liquid after will decomposing is behind concentrated original volume 1/3rd, and lyophilize is to moisture content below 4%, and abrasive dust gets final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410004604 CN1657634A (en) | 2004-02-18 | 2004-02-18 | Process for preparing active lactoferritin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410004604 CN1657634A (en) | 2004-02-18 | 2004-02-18 | Process for preparing active lactoferritin |
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| CN1657634A true CN1657634A (en) | 2005-08-24 |
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| CN 200410004604 Pending CN1657634A (en) | 2004-02-18 | 2004-02-18 | Process for preparing active lactoferritin |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101235408B (en) * | 2007-11-27 | 2011-01-05 | 江南大学 | Method for preparing Lactoferrins antibiotic peptide by enzyme method |
| CN110237272A (en) * | 2019-06-25 | 2019-09-17 | 武汉大学苏州研究院 | Suitable for the bimodal tumor imaging nano-probe of MRI/NIR-II, preparation method and application |
-
2004
- 2004-02-18 CN CN 200410004604 patent/CN1657634A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101235408B (en) * | 2007-11-27 | 2011-01-05 | 江南大学 | Method for preparing Lactoferrins antibiotic peptide by enzyme method |
| CN110237272A (en) * | 2019-06-25 | 2019-09-17 | 武汉大学苏州研究院 | Suitable for the bimodal tumor imaging nano-probe of MRI/NIR-II, preparation method and application |
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