CN1648252A - Wheat TaGI1 gene and its cloning and use - Google Patents

Wheat TaGI1 gene and its cloning and use Download PDF

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Publication number
CN1648252A
CN1648252A CN 200410035916 CN200410035916A CN1648252A CN 1648252 A CN1648252 A CN 1648252A CN 200410035916 CN200410035916 CN 200410035916 CN 200410035916 A CN200410035916 A CN 200410035916A CN 1648252 A CN1648252 A CN 1648252A
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gene
tagi1
wheat
cdna
plant
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CN1291020C (en
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张宪省
赵翔宇
李祥
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Shandong Agricultural University
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Shandong Agricultural University
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Abstract

The present invention relates to the cloning, recombination, function analysis and application of wheat TaGI1 gene, and belongs to the field of molecular biology and biotechnology. Through extracting total RNA from ear of wheat, inversely transcribing 2 microgram of total RNA into cDNA; designing one pair of amphoteric primers based on conservative G1 (Arabidopsis thalianum) and OsGI (rice) nucleotide sequence for conventional PCR, connecting PCR product to pGEM-T vector, converting DH5alpha cell, determining sequence; and fast amplifying in 3' end and 5' end, whole length cDNA is obtained. Three sequences with very high homogeny are obtained and named as TaGI1, TaGI2 and TaGI3 separately. Positive sense expression vector is further constituted with whole length TaGI1 cDNA and used in transforming Arabidopsis thalianum to result in antecedent florescence. Therefore, the gene may be used to regulate the growth period of other crops effectively.

Description

Wheat TaGI1 gene and clone thereof and application
(1) technical field:
The present invention relates to clone, reorganization, functional analysis and the application thereof of TaGI1 gene in the wheat, belong to molecular biology and biological technical field.
(2) background technology:
Plant is by the transformation of vegetative growth phase to generative growth phase, flower change, be the committed step that plant is finished whole life cycle.The flowering of plant time is controlled by inherent ambient signal of growing signal and outside jointly.Studies show that in the last few years mainly contains four approach controls and blooms, and is respectively photoperiod promotion approach, independently promotes approach, vernalization to promote approach and GA to promote approach.With Arabidopis thaliana and paddy rice isotype plant is object, participates in some important gene that the photoperiod promotes approach, as GI, CO, is come out by the clone in succession.Caused after the Arabidopis thaliana GI transgenation its mutant under the long day condition late blooming (Koomneef et al., Mol.Gen.Genet.1991,229:57-66).People such as Curtis oppositely change the segment of Arabidopis thaliana GI gene in the radish, the result caused transgenosis radish bolting and bloom all and to postpone (Curtis et al., Transgenic Res.2002,11:249-256).The GI gene (OsGI) of paddy rice change in the paddy rice after the overexpression render transgenic paddy rice still under the short day condition, all bloom evening under the long day condition (Hayama et al.Nature 2003,422:719-22).Studies have shown that the GI gene directly affects the flowering time of plant at the intravital expression level of plant, and then influence the breeding cycle of plant.Therefore, can utilize plant transgenic technology to regulate the growth and development of plant cycle.
The long day plant wheat is one of important crops in the world.One of traditional main breeding technique is to introduce a fine variety, and just the good crop varieties in other places or foreign country is introduced the locality, uses as commercial variety or breeding material.But of will finish from the seed to the seed of wheat grows the cycle, must be by vernalization stage and photostage, otherwise just can not the normally solid output of obtaining.But because weather condition such as the illumination of all parts of the world, temperature are widely different, so the work of introducing a fine variety is very restricted.If can utilize the biotechnology means to regulate growing the cycle of wheat, the scope of introducing a fine variety so just can greatly enlarge.
In view of the above, the present invention utilizes reverse transcription-polymerase chain reaction to isolate the homologous gene of three GI genes from wheat.Further make up the sense expression vector of TaGI1, transformation mode plant Arabidopis thaliana, transfer-gen plant is carried about the last week than wild-type contrast flowering time.This gene can be used for plant genetic and transform, and regulates the growth and development of plant cycle.
(3) summary of the invention:
The present invention isolates the full-length cDNA of three TaGI genes first from wheat, difference called after TaGI1, TaGI2, TaGI3, and the TaGI1 full-length cDNA is connected on the expression vector, utilize the During Agrobacterium arabidopsis thaliana transformation, the flowering time of the transfer-gen plant of acquisition is carried about the last week than the wild-type contrast.
The open reading frame of wheat TaGI1 (AF543844) gene is 3465bp, coding 1155aa.The aminoacid sequence of this genes encoding is retrieved discovery in GeneBank, this gene and GI (Arabidopis thaliana, AY682088) and OsGIP (paddy rice AJ133787) has higher homology (seeing accompanying drawing 1), and its numerical value is 63% and 87% amino acid identity.This result shows that this gene is the homologous gene of Arabidopis thaliana GI gene in wheat.This gene order is as follows:
Sequence table
(1) information of SEQ ID NO.1
(a) sequence signature
*Length: 4012 base pairs
*Type: nucleic acid
*Chain: two strands
*Topological framework: linearity
(b) molecule type: cDNA
(c) suppose: not
(d) antisense: not
(e) initial source: wheat
(f) sequence description: SEQ ID NO.1
1?ctccgtattg?gagcgctcct?gaccgagaga?cgttaagcgc?ctatggttgg?aactggttga 60
61?tatgatatct?ggtcacattg?tgggatgcaa?tcccccctta?gtggggggtg?attgaagaag 120
121?tcaacaatag?gaggacttgc?atcttgctga?agttttctgc?ttctgagttt?cttgagcagg 180
181?ctcttaaact?accaagtatg?tcagtgtcaa?atgggaagtg?gattgatggg?ctccagttct 240
241?catcactgtt?ctggcccccg?ccacacgatg?tgcagcagaa?acaggcacaa?attttagcct 300
301?atgtcgagta?ctttgggcag?ttcacatctg?acagcgagca?attcccggag?gatgtagctc 360
361?agctaattca?aagttgctat?ccatcaaaag?aaaagcggtt?ggtagatgaa?gtattagcaa 420
421?cttttgttct?ccatcaccct?gaacatggtc?atgcagttgt?acatccaatt?ctttcacgca 480
481?tcatagatgg?gacactgagt?tatgatagtc?atggttcccc?attcaattcc?ttcatctctt 540
541?tatttaccca?aagttctgag?aaagagtact?cagagcagtg?ggccttggcc?tgtggagaaa 600
601?ttcttagagt?tctaactcac?tacaacaggc?caatctttaa?agttgcagac?tgtaaccacc 660
661?agatcagacc?aggccacagc?aagttgtttt?gcacagagaa?agcaattact?cttccaggaa 720
721?atgaacccga?agggaagcca?ttgaggccat?tatctccttg?gatcacagat?attgtgctaa 780
781?ctgcaccttt?gggcattaga?agcgactatt?ttagatggtg?tggtggagtt?atgggaaaat 840
841?acgcagctgg?tggagaattg?aagcctccaa?caactgctta?cagccgagga?gctggcaagc 900
901?acccacaact?catgccatcc?accccaagat?gggctgttgc?caatggagct?ggagttattt 960
961?taagtgtctg?tgatgaggaa?gtagctcgtt?atgagacagc?aaacttaacc?gcggcagctg?1020
1021?ttcccgccct?tctgctacct?ccaccgacaa?cacccttgga?tgagcatttg?gtggcagggc?1080
1081?taccccctct?cgaaccatac?gctcgcttgt?ttcatagata?ctacgcaatt?gccacaccaa?1140
1141?gtgctacgca?aagattgctt?tttggtcttc?ttgaagcacc?accgtcatgg?gctccagatg?1200
1201?cacttgatgc?agcagttcag?cttgttgaac?tccttcgggc?agctgaagat?tatgctactg?1260
1261?gcatgcggct?tccaaaaaat?tggttgcatc?ttcatttctt?gcgtgcgatt?ggaactgcaa?1320
1321?tgtctatgag?ggctggtatt?gctgctgata?cagctgctgc?gttgcttttt?cgcatactat?1380
1381?cccaaccaat?gttgcttttt?cctccactaa?ggcatgctga?aggagttgaa?gtgcaacatg?1440
1441?aaccactggg?tggctatgta?tcatcataca?aaagacagct?ggaagttcct?gcatctgaaa?1500
1501?ccacaattga?tgccactgca?caaggcattg?cttccttgct?gtgtgctcat?ggtcctgatg?1560
1561?ttgagtggag?aatatgtacc?atctgggaag?ctgcctatgg?tttgttacct?ctgaattcat?1620
1621?cagcagttga?tttacccgaa?atcgttgtag?ctgctccgct?tcagccacct?actttgtcat?1680
1681?ggagcctata?cttgccactg?ttgaaagtat?ttgagtatct?acctcgtgga?agtccatctg?1740
1741?aagcatgcct?tatgagaata?tttgtggcaa?cagttgaagc?tatactcaga?agaactttcc?1800
1801?cttcggaaac?ctctgaatca?tctaaaagac?caagaagtca?atccaagaac?cttgctgttg?1860
1861?ctgaactccg?tacgatgata?cattcactct?ttgttgaatc?atgtgcttca?atgaaccttg?1920
1921?cttcccggtt?gttgtttgta?gtattaactg?tttgcgtcag?tcatcaagct?ttgccagggg?1980
1981?gcagcaaaag?accaactggt?agtgaaaacc?attcttctga?ggaggccact?gaggacccaa?2040
2041?gattaaccaa?tggaagaaat?agggtcaaga?agaaacaagg?gcctgttggt?acatttgact?2100
2101?cgtatgtgct?ggccgctgtt?tgtgccttat?cttgcgagct?tcagctgttc?cctatccttt?2160
2161?gcaagagcgc?aacaaactca?aatgtaaaag?actctataaa?gatcctgaag?cctggaaaaa?2220
2221?acaatgggat?cagtaatgag?ctacagaaca?gcattagctc?agcaattctc?catactcgta?2280
2281?gaattcttgg?catcctggaa?gctcttttct?ccttgaagcc?atcatcagtt?ggtacctcct?2340
2341?ggaactatag?ttcaaatgag?atagttgcag?cggctatggt?tgccgctcat?gcttctgagt?2400
2401?tatttcgccg?gtcgaaggca?tgcctaaatg?cactatcttc?cctgaagcga?tgtaagtggg?2460
2461?atgctgagat?ttctaccagg?gcatcatccc?tctaccattt?gatcgacttg?catggtaaaa?2520
2521?ctgtgtcctc?catcgtgaac?aaagctgagc?ctctagaagc?tcacctgact?tttacatcag?2580
2581?taaagagaga?tgatgaacaa?cacattgagg?aaaatggcac?cagctcatcg?ggtagtggca?2640
2641?acttggaaaa?gaagaatggt?tcagcctcac?acatgaaaaa?tggtttgtca?agaccactct?2700
2701?tgaaatgctc?agaagaggct?aggcgaaatg?gtaatgttgc?aagtacatcc?gggaaagttc?2760
2761?ctgcaacttt?acaggctgaa?gcttctgatt?tggcgaactt?ccttaccatg?gataggaatg?2820
2821?gcggttatcg?aggctctcag?actctcctaa?gatctgttat?ctcagaaaaa?caggaactaa?2880
2881?gcttctctgt?tgtctcattg?ctctggcata?agcttattgc?atctcctgaa?acgcagatgt?2940
2941?ctgcagaaag?tacatcagct?catcaaggtt?ggagaaaggt?tgtagatgct?ctttgtgatg?3000
3001?ttgtttcagc?ctcaccagcc?aaggcttcaa?ctgctattgt?tctgcaggct?gagaaggacc?3060
3061?tacagccctg?gattgctcga?gatgatgagg?aaggtcagaa?gatgtggaga?gtcaaccagc?3120
3121?gaatagttaa?actgatagct?gagcttatga?ggaaccatga?tagcccagaa?gcattgataa?3180
3181?ttcttgctag?tgcttcagac?cttctgcttc?gtgctacaga?tgggatgctt?gttgatggtg?3240
3241?aagcttgtac?cttgcctcag?ttagagctcc?tggaagtaac?tgctagagct?attcatctca?3300
3301?tcgttgaatg?gggagaccca?ggtgtagcag?ttgctgatgg?cctctcaaat?ctgttaaagt?3360
3361?gccgtctatc?gcctaccatc?cgctgcctct?cccacgctag?cgcacacgta?cgtgcgctca?3420
3421?gcatgtccgt?cctccgtgac?atcttgaaca?gtggaccact?aggttccact?aagatcattc?3480
3481?aaggcgagca?acggaatggc?atccaaagcc?cgacctacca?atgcgcggca?gcaaacacgg?3540
3541?tgaactggca?agcggatgtc?gagagatgta?tagactggga?agcccgcagc?cgccgcgcca?3600
3601?ccgggatgac?cctcgccttc?ctcaccgctg?cggctaacga?gctcggctgc?ccccttcctt?3660
3661?gctgacaagg?ccatatttgg?agctgacaat?cagcgacact?tgacagttgg?cgcgagcagt?3720
3721?tgctgcatgg?tcagcgagca?ggatggctaa?ttaatccctt?gctcaaggat?ggcttcccag?3780
3781?tctgccctgc?ccccatgatg?tgattcaaaa?ctgatgtata?ttagctgatg?ttcccaatca?3840
3841?tacggagctt?tgctcccccg?gtgtgatttt?aacttgcaat?ctgacgttag?atgttcaagc?3900
3901?atattgaaac?tgcttgtgct?gtaacttgta?ttcatgatgt?aaccgaaaga?tgtccgctat?3960
3961?ggtaaatgaa?gacatgtcat?ttttcgttct?ccaaaaaaaa?aaaaaaaaaa?aa 4012
(2) information of SEQ ID NO.2
(a) sequence signature
*Length: 1155 amino acid
*Type: amino acid
*Chain: strand
*Topological framework: linearity
(b) molecule type: protein
(c) sequence description
0001 MSVSNGKWID GLQFSSLFWP PPHDVQQKQA QILAYVEYFG QFTSDSEQFP EDVAQLIQSC 60
0061 YPSKEKRLVD EVLATFVLHH PEHGHAVVHP ILSRIIDGTL SYDSHGSPFN SFISLFTQSS 120
0121 EKEYSEQWAL ACGEILRVLT HYNRPIFKVA DCNHQIRPGH SKLFCTEKAI TLPGNEPEGK 180
0181 PLRPLSPWIT DIVLTAPLGI RSDYFRWCGG VMGKYAAGGE LKPPTTAYSR GAGKHPQLMP 240
0241 STPRWAVANG AGVILSVCDE EVARYETANL TAAAVPALLL PPPTTPLDEH LVAGLPPLEP 300
0301 YARLFHRYYA IATPSATQRL LFGLLEAPPS WAPDALDAAV QLVELLRAAE DYATGMRLPK 360
0361 NWLHLHFLRA IGTAMSMRAG IAADTAAALL FRILSQPMLL FPPLRHAEGV EVQHEPLGGY 420
0421 VSSYKRQLEV PASETTIDAT AQGIASLLCA HGPDVEWRIC TIWEAAYGLL PLNSSAVDLP 480
0481 EIVVAAPLQP PTLSWSLYLP LLKVFEYLPR GSPSEACLMR IFVATVEAIL RRTFPSETSE 540
0541 SSKRPRSQSK NLAVAELRTM IHSLFVESCA SMNLASRLLF VVLTVCVSHQ ALPGGSKRPT 600
0601 GSENHSSEEA TEDPRLTNGR NRVKKKQGPV GTFDSYVLAA VCALSCELQL FPILCKSATN 660
0661 SNVKDSIKIL KPGKNNGISN ELQNSISSAI LHTRRILGIL EALFSLKPSS VGTSWNYSSN 720
0721 EIVAAAMVAA HASELFRRSK ACLNALSSLK RCKWDAEIST RASSLYHLID LHGKTVSSIV 780
0781 NKAEPLEAHL TFTSVKRDDE QHIEENGTSS SGSGNLEKKN GSASHMKNGL SRPLLKCSEE 840
0841 ARRNGNVAST SGKVPATLQA EASDLANFLT MDRNGGYRGS QTLLRSVISE KQELSFSVVS 900
0901 LLWHKLIASP ETQMSAESTS AHQGWRKVVD ALCDVVSASP AKASTAIVLQ AEKDLQPWIA 960
0961 RDDEEGQKMW RVNQRIVKLI AELMRNHDSP EALIILASAS DLLLRATDGM LVDGEACTLP 1020
1021 QLELLEVTAR AIHLIVEWGD PGVAVADGLS NLLKCRLSPT IRCLSHASAH VRALSMSVLR 1080
1081 DILNSGPLGS TKIIQGEQRN GIQSPTYQCA AANTVNWQAD VERCIDWEAR SRRATGMTLA 1140
1141 FLTAAANELG CPLPC 1155
Extracting genomic dna from the wheat spire, carry out enzyme with different restriction enzymes respectively then and cut, is that the template synthesising probing needle carries out the Southern hybridization analysis with one section sequence of TaGI1.The result is presented at the homologous gene that has three GI genes in the wheat cdna group, and these three genes are TaGI1, TaGI2 and TaGI3 (accompanying drawing 3) just.In addition, show that the TaGI1 gene all has expression (seeing accompanying drawing 2) in each histoorgan except that endosperm by the Northen hybridization analysis that carries out for different wheat histoorgans.
Utilize the osmose process arabidopsis thaliana transformation, the seed of results screens containing on the LB solid medium of kantlex, with the resistance seedling that filters out on the GM substratum with the wild-type co-cultivation.The result shows, compares with wild-type, and transfer-gen plant is equal early flowering (seeing accompanying drawing 4) under long day and short day condition.
According to above-mentioned technology, from wheat, be separated to TaGI1, TaGI2, TaGI3 gene, the overexpression of TaGI1 gene can cause transgenic arabidopsis to be carried about the last week blooming.Wheat is global important food crop, changes this gene over to wheat and makes its expression can regulate the cycle of growing and the flowering time of wheat, has very important economic benefit and social benefit.
(4) description of drawings:
The amino acid identity of OsGIP is relatively in GI and the paddy rice in TaGI1, TaGI2, TaGI3 and the Arabidopis thaliana in Fig. 1 wheat.
The Northern hybridization of Fig. 2 TaGI1 gene in the different tissues organ.R (root), S (stem), L (leaf), SP (little spike primordium), Spi (small ear), Em (embryo), En (endosperm).
Fig. 3 TaGI gene Southem hybridization.X，Xba?I；B，BamH?I；N，NdeI。
Fig. 4 transgenic arabidopsis is equal early flowering under long day and short day condition.
(5) concrete invention embodiment:
Embodiment 1: the cloning process of wheat TaGI1 gene
(1) extraction of RNA: adopt single stage method or RNAkit to extract total RNA
(2) A article one chain is synthetic: get the total RNA of 2 μ g, add 5 * reaction buffer, 4 μ l, 10mM thymus nucleic acid (dNTP) 2 μ l, ribonuclease inhibitor (40-200u/ μ l) 0.5 μ l, primer oligodT (1 μ g/ μ l) 1 μ l, ThermoScript II (10u/ μ l) 2 μ l, 42 ℃ were reacted 60 minutes, place 10 minutes termination reactions, be diluted to 200 μ l for 85 ℃.
(3) PCR reaction: polymerase chain reaction (PCR) reagent and condition are:
At first following reagent is mixed:
10 * reaction buffer, 5 μ l
Deoxynucleoside acid mixture (dNTP) 4 μ l
Forward primer (5 μ M) 4 μ l
Reverse primer (5 μ M) 4 μ l
Template cDNA 4 μ l
TaqDNA polysaccharase 0.5 μ l
Cumulative volume 50 μ l
The PCR reaction conditions is: 94 ℃ 3 minutes; Enter following circulation then: 94 ℃ 1 minute, 56 ℃ 1 minute, 72 ℃ 1 minute, totally 35 circulations; Last 72 ℃ were extended 10 minutes.
(4) gene clone: get 2 μ lPCR products and be connected with the pGEM-T carrier, operation steps is undertaken by Promega company product pGEM-T and pGEM-T easy Vector system specification sheets.Connect product transformed into escherichia coli DH5 α bacterial strain then, be coated with grow overnight on the LB flat board that contains penbritin (100 μ g/ml) of 5-bromo-4-chloro-3-indoles-β-D-galactoside and X-gal on the surface.The picking white colony, overnight incubation in the LB liquid nutrient medium.
(5) extraction of plasmid DNA: alkaline process extracts plasmid DNA.
(6) sequencing: originally be operated in Dalian Bao Bio-Engineering Company and carry out.
The separation of (7) 5 ' sequences: the SMAR TRACE cDNA AmplificationKit specification sheets by Clontech company carries out
(8) homology retrieval: utilize BLAST software that the sequence in isolated sequence and the gene library is compared.
Embodiment 2: the nucleotide sequence and the aminoacid sequence of wheat TaGI1 gene seen " summary of the invention " part respectively.
Embodiment 3: the structure of expression vector
(1) according to the nucleotide sequence of isolated TaGI1 gene, design primer:
Forward primer: 5 '-TATTCTAGACGAGGTGGCGACCGGCATGG-3 '
Reverse primer: 5 '-GACGAGCTCCTTAACTACGGTCTTCTGC-3 '
CDNA with total RNA reverse transcription of root is a template, carries out the polymerase chain reaction.
(2) get 2 μ l PCR and be connected with the pGEM-T carrier, operation steps is undertaken by Promega company product pGEM-T and pGEM-T easy Vector system specification sheets.Transformed into escherichia coli DH5 α bacterial strain then is coated with grow overnight on the LB flat board that contains penbritin (100 μ g/ml) of 5-bromo-4-chloro-3-indoles-β-D-galactoside and X-gal on the surface.The picking white colony, overnight incubation in the LB liquid nutrient medium.Alkaline process extracts plasmid DNA, carries out sequencing.
(3) with the BamH1 restriction enzyme this gene is downcut from the pGEM-T carrier, be connected with the pROK II that the same enzyme enzyme is cut.Connect product and transform DH5 α cell, cultivate containing on the LB solid plate of penbritin then, bacterium colony is carried out PCR identifies and the restriction analysis of plasmid DNA.
(4) expression vector that builds is transformed Agrobacterium GV3101.
Embodiment 4: gene function analysis
(1) plantation Arabidopis thaliana.
(2) the Agrobacterium mono-clonal identified of picking is in the LB liquid nutrient medium that contains 50 μ g/ml kantlex, 28 ℃ of shaking culture.
(3) centrifugal collection thalline, precipitation is with permeating substratum (5% sucrose, 0.1M MgCl 2, 0.5%SilwetL-77) suspend bacterium liquid OD 600About 0.8.
(4) the Arabidopis thaliana inflorescence is immersed in the penetrating fluid, soaked 5 minutes.
(5) Shou Huo seed obtains resistant plant in screening culture medium (1 * MS salt, 1% sucrose, pH5.7,0.8% agar, kantlex 30 μ g/ml) screening.
(6) T3 is cultivated on the GM substratum for homozygous lines seed kind, plant wild-type simultaneously and compare, the number of statistics lotus throne leaf was to measure flowering time when plant was taken out inflorescence.The results are shown in accompanying drawing 4.
Sequence table
＜110〉Shandong Agricultural University
＜120〉wheat TaGI1 gene and clone thereof and application
<160>1
<170>patent?In?3.1
<210>1
<211>4012
<212>cDNA
＜213〉wheat (Triticum aestivum L.)
<221>1-4012
<400>1
1?CTCCGTATTG?GAGCGCTCCT?GACCGAGAGA?CGTTAAGCGC?CTATGGTTGG?AACTGGTTGA 60
61?TATGATATCT?GGTCACATTG?TGGGATGCAA?TCCCCCCTTA?GTGGGGGGTG?ATTGAAGAAG 120
121?TCAACAATAG?GAGGACTTGC?ATCTTGCTGA?AGTTTTCTGC?TTCTGAGTTT?CTTGAGCAGG 180
181?CTCTTAAACT?ACCAAGTATG?TCAGTGTCAA?ATGGGAAGTG?GATTGATGGG?CTCCAGTTCT 240
241?CATCACTGTT?CTGGCCCCCG?CCACACGATG?TGCAGCAGAA?ACAGGCACAA?ATTTTAGCCT 300
301?ATGTCGAGTA?CTTTGGGCAG?TTCACATCTG?ACAGCGAGCA?ATTCCCGGAG?GATGTAGCTC 360
361?AGCTAATTCA?AAGTTGCTAT?CCATCAAAAG?AAAAGCGGTT?GGTAGATGAA?GTATTAGCAA 420
421?CTTTTGTTCT?CCATCACCCT?GAACATGGTC?ATGCAGTTGT?ACATCCAATT?CTTTCACGCA 480
481?TCATAGATGG?GACACTGAGT?TATGATAGTC?ATGGTTCCCC?ATTCAATTCC?TTCATCTCTT 540
541?TATTTACCCA?AAGTTCTGAG?AAAGAGTACT?CAGAGCAGTG?GGCCTTGGCC?TGTGGAGAAA 600
601?TTCTTAGAGT?TCTAACTCAC?TACAACAGGC?CAATCTTTAA?AGTTGCAGAC?TGTAACCACC 660
661?AGATCAGACC?AGGCCACAGC?AAGTTGTTTT?GCACAGAGAA?AGCAATTACT?CTTCCAGGAA 720
721?ATGAACCCGA?AGGGAAGCCA?TTGAGGCCAT?TATCTCCTTG?GATCACAGAT?ATTGTGCTAA 780
781?CTGCACCTTT?GGGCATTAGA?AGCGACTATT?TTAGATGGTG?TGGTGGAGTT?ATGGGAAAAT 840
841?ACGCAGCTGG?TGGAGAATTG?AAGCCTCCAA?CAACTGCTTA?CAGCCGAGGA?GCTGGCAAGC 900
901?ACCCACAACT?CATGCCATCC?ACCCCAAGAT?GGGCTGTTGC?CAATGGAGCT?GGAGTTATTT 960
961?TAAGTGTCTG?TGATGAGGAA?GTAGCTCGTT?ATGAGACAGC?AAACTTAACC?GCGGCAGCTG?1020
1021?TTCCCGCCCT?TCTGCTACCT?CCACCGACAA?CACCCTTGGA?TGAGCATTTG?GTGGCAGGGC?1080
1081?TACCCCCTCT?CGAACCATAC?GCTCGCTTGT?TTCATAGATA?CTACGCAATT?GCCACACCAA?1140
1141?GTGCTACGCA?AAGATTGCTT?TTTGGTCTTC?TTGAAGCACC?ACCGTCATGG?GCTCCAGATG?1200
1201?CACTTGATGC?AGCAGTTCAG?CTTGTTGAAC?TCCTTCGGGC?AGCTGAAGAT?TATGCTACTG?1260
1261?GCATGCGGCT?TCCAAAAAAT?TGGTTGCATC?TTCATTTCTT?GCGTGCGATT?GGAACTGCAA?1320
1321?TGTCTATGAG?GGCTGGTATT?GCTGCTGATA?CAGCTGCTGC?GTTGCTTTTT?CGCATACTAT?1380
1381?CCCAACCAAT?GTTGCTTTTT?CCTCCACTAA?GGCATGCTGA?AGGAGTTGAA?GTGCAACATG?1440
1441?AACCACTGGG?TGGCTATGTA?TCATCATACA?AAAGACAGCT?GGAAGTTCCT?GCATCTGAAA?1500
1501?CCACAATTGA?TGCCACTGCA?CAAGGCATTG?CTTCCTTGCT?GTGTGCTCAT?GGTCCTGATG?1560
1561?TTGAGTGGAG?AATATGTACC?ATCTGGGAAG?CTGCCTATGG?TTTGTTACCT?CTGAATTCAT?1620
1621?CAGCAGTTGA?TTTACCCGAA?ATCGTTGTAG?CTGCTCCGCT?TCAGCCACCT?ACTTTGTCAT?1680
1681?GGAGCCTATA?CTTGCCACTG?TTGAAAGTAT?TTGAGTATCT?ACCTCGTGGA?AGTCCATCTG?1740
1741?AAGCATGCCT?TATGAGAATA?TTTGTGGCAA?CAGTTGAAGC?TATACTCAGA?AGAACTTTCC?1800
1801?CTTCGGAAAC?CTCTGAATCA?TCTAAAAGAC?CAAGAAGTCA?ATCCAAGAAC?CTTGCTGTTG?1860
1861?CTGAACTCCG?TACGATGATA?CATTCACTCT?TTGTTGAATC?ATGTGCTTCA?ATGAACCTTG?1920
1921?CTTCCCGGTT?GTTGTTTGTA?GTATTAACTG?TTTGCGTCAG?TCATCAAGCT?TTGCCAGGGG?1980
1981?GCAGCAAAAG?ACCAACTGGT?AGTGAAAACC?ATTCTTCTGA?GGAGGCCACT?GAGGACCCAA?2040
2041?GATTAACCAA?TGGAAGAAAT?AGGGTCAAGA?AGAAACAAGG?GCCTGTTGGT?ACATTTGACT?2100
2101?CGTATGTGCT?GGCCGCTGTT?TGTGCCTTAT?CTTGCGAGCT?TCAGCTGTTC?CCTATCCTTT?2160
2161?GCAAGAGCGC?AACAAACTCA?AATGTAAAAG?ACTCTATAAA?GATCCTGAAG?CCTGGAAAAA?2220
2221?ACAATGGGAT?CAGTAATGAG?CTACAGAACA?GCATTAGCTC?AGCAATTCTC?CATACTCGTA?2280
2281?GAATTCTTGG?CATCCTGGAA?GCTCTTTTCT?CCTTGAAGCC?ATCATCAGTT?GGTACCTCCT?2340
2341?GGAACTATAG?TTCAAATGAG?ATAGTTGCAG?CGGCTATGGT?TGCCGCTCAT?GCTTCTGAGT?2400
2401?TATTTCGCCG?GTCGAAGGCA?TGCCTAAATG?CACTATCTTC?CCTGAAGCGA?TGTAAGTGGG?2460
2461?ATGCTGAGAT?TTCTACCAGG?GCATCATCCC?TCTACCATTT?GATCGACTTG?CATGGTAAAA?2520
2521?CTGTGTCCTC?CATCGTGAAC?AAAGCTGAGC?CTCTAGAAGC?TCACCTGACT?TTTACATCAG?2580
2581?TAAAGAGAGA?TGATGAACAA?CACATTGAGG?AAAATGGCAC?CAGCTCATCG?GGTAGTGGCA?2640
2641?ACTTGGAAAA?GAAGAATGGT?TCAGCCTCAC?ACATGAAAAA?TGGTTTGTCA?AGACCACTCT?2700
2701?TGAAATGCTC?AGAAGAGGCT?AGGCGAAATG?GTAATGTTGC?AAGTACATCC?GGGAAAGTTC?2760
2761?CTGCAACTTT?ACAGGCTGAA?GCTTCTGATT?TGGCGAACTT?CCTTACCATG?GATAGGAATG?2820
2821?GCGGTTATCG?AGGCTCTCAG?ACTCTCCTAA?GATCTGTTAT?CTCAGAAAAA?CAGGAACTAA?2880
2881?GCTTCTCTGT?TGTCTCATTG?CTCTGGCATA?AGCTTATTGC?ATCTCCTGAA?ACGCAGATGT?2940
2941?CTGCAGAAAG?TACATCAGCT?CATCAAGGTT?GGAGAAAGGT?TGTAGATGCT?CTTTGTGATG?3000
3001?TTGTTTCAGC?CTCACCAGCC?AAGGCTTCAA?CTGCTATTGT?TCTGCAGGCT?GAGAAGGACC?3060
3061?TACAGCCCTG?GATTGCTCGA?GATGATGAGG?AAGGTCAGAA?GATGTGGAGA?GTCAACCAGC?3120
3121?GAATAGTTAA?ACTGATAGCT?GAGCTTATGA?GGAACCATGA?TAGCCCAGAA?GCATTGATAA?3180
3181?TTCTTGCTAG?TGCTTCAGAC?CTTCTGCTTC?GTGCTACAGA?TGGGATGCTT?GTTGATGGTG?3240
3241?AAGCTTGTAC?CTTGCCTCAG?TTAGAGCTCC?TGGAAGTAAC?TGCTAGAGCT?ATTCATCTCA?3300
3301?TCGTTGAATG?GGGAGACCCA?GGTGTAGCAG?TTGCTGATGG?CCTCTCAAAT?CTGTTAAAGT?3360
3361?GCCGTCTATC?GCCTACCATC?CGCTGCCTCT?CCCACGCTAG?CGCACACGTA?CGTGCGCTCA?3420
3421?GCATGTCCGT?CCTCCGTGAC?ATCTTGAACA?GTGGACCACT?AGGTTCCACT?AAGATCATTC?3480
3481?AAGGCGAGCA?ACGGAATGGC?ATCCAAAGCC?CGACCTACCA?ATGCGCGGCA?GCAAACACGG?3540
3541?TGAACTGGCA?AGCGGATGTC?GAGAGATGTA?TAGACTGGGA?AGCCCGCAGC?CGCCGCGCCA?3600
3601?CCGGGATGAC?CCTCGCCTTC?CTCACCGCTG?CGGCTAACGA?GCTCGGCTGC?CCCCTTCCTT?3660
3661?GCTGACAAGG?CCATATTTGG?AGCTGACAAT?CAGCGACACT?TGACAGTTGG?CGCGAGCAGT?3720
3721?TGCTGCATGG?TCAGCGAGCA?GGATGGCTAA?TTAATCCCTT?GCTCAAGGAT?GGCTTCCCAG?3780
3781?TCTGCCCTGC?CCCCATGATG?TGATTCAAAA?CTGATGTATA?TTAGCTGATG?TTCCCAATCA?3840
3841?TACGGAGCTT?TGCTCCCCCG?GTGTGATTTT?AACTTGCAAT?CTGACGTTAG?ATGTTCAAGC?3900
3901?ATATTGAAAC?TGCTTGTGCT?GTAACTTGTA?TTCATGATGT?AACCGAAAGA?TGTCCGCTAT?3960
3961?GGTAAATGAA?GACATGTCAT?TTTTCGTTCT?CCAAAAAAAA?AAAAAAAAAA?AA 4012

Claims (2)

1. the gene TaGI1 of a clone control wheat flower development is characterized in that it has the sequence shown in following:
01?CTCCGTATTG?GAGCGCTCCT?GACCGAGAGA?CGTTAAGCGC?CTATGGTTGG?AACTGGTTGA 60
61?TATGATATCT?GGTCACATTG?TGGGATGCAA?TCCCCCCTTA?GTGGGGGGTG?ATTGAAGAAG 120
121?TCAACAATAG?GAGGACTTGC?ATCTTGCTGA?AGTTTTCTGC?TTCTGAGTTT?CTTGAGCAGG 180
181?CTCTTAAACT?ACCAAGTATG?TCAGTGTCAA?ATGGGAAGTG?GATTGATGGG?CTCCAGTTCT 240
241?CATCACTGTT?CTGGCCCCCG?CCACACGATG?TGCAGCAGAA?ACAGGCACAA?ATTTTAGCCT 300
301?ATGTCGAGTA?CTTTGGGCAG?TTCACATCTG?ACAGCGAGCA?ATTCCCGGAG?GATGTAGCTC 360
361?AGCTAATTCA?AAGTTGCTAT?CCATCAAAAG?AAAAGCGGTT?GGTAGATGAA?GTATTAGCAA 420
421?CTTTTGTTCT?CCATCACCCT?GAACATGGTC?ATGCAGTTGT?ACATCCAATT?CTTTCACGCA 480
481?TCATAGATGG?GACACTGAGT?TATGATAGTC?ATGGTTCCCC?ATTCAATTCC?TTCATCTCTT 540
541?TATTTACCCA?AAGTTCTGAG?AAAGAGTACT?CAGAGCAGTG?GGCCTTGGCC?TGTGGAGAAA 600
601?TTCTTAGAGT?TCTAACTCAC?TACAACAGGC?CAATCTTTAA?AGTTGCAGAC?TGTAACCACC 660
661?AGATCAGACC?AGGCCACAGC?AAGTTGTTTT?GCACAGAGAA?AGCAATTACT?CTTCCAGGAA 720
721?ATGAACCCGA?AGGGAAGCCA?TTGAGGCCAT?TATCTCCTTG?GATCACAGAT?ATTGTGCTAA 780
781?CTGCACCTTT?GGGCATTAGA?AGCGACTATT?TTAGATGGTG?TGGTGGAGTT?ATGGGAAAAT 840
841?ACGCAGCTGG?TGGAGAATTG?AAGCCTCCAA?CAACTGCTTA?CAGCCGAGGA?GCTGGCAAGC 900
901?ACCCACAACT?CATGCCATCC?ACCCCAAGAT?GGGCTGTTGC?CAATGGAGCT?GGAGTTATTT 960
961?TAAGTGTCTG?TGATGAGGAA?GTAGCTCGTT?ATGAGACAGC?AAACTTAACC?GCGGCAGCTG?1020
1021?TTCCCGCCCT?TCTGCTACCT?CCACCGACAA?CACCCTTGGA?TGAGCATTTG?GTGGCAGGGC?1080
1081?TACCCCCTCT?CGAACCATAC?GCTCGCTTGT?TTCATAGATA?CTACGCAATT?GCCACACCAA?1140
1141?GTGCTACGCA?AAGATTGCTT?TTTGGTCTTC?TTGAAGCACC?ACCGTCATGG?GCTCCAGATG?1200
1201?CACTTGATGC?AGCAGTTCAG?CTTGTTGAAC?TCCTTCGGGC?AGCTGAAGAT?TATGCTACTG?1260
1261?GCATGCGGCT?TCCAAAAAAT?TGGTTGCATC?TTCATTTCTT?GCGTGCGATT?GGAACTGCAA?1320
1321?TGTCTATGAG?GGCTGGTATT?GCTGCTGATA?CAGCTGCTGC?GTTGCTTTTT?CGCATACTAT?1380
1381?CCCAACCAAT?GTTGCTTTTT?CCTCCACTAA?GGCATGCTGA?AGGAGTTGAA?GTGCAACATG?1440
1441?AACCACTGGG?TGGCTATGTA?TCATCATACA?AAAGACAGCT?GGAAGTTCCT?GCATCTGAAA?1500
1501?CCACAATTGA?TGCCACTGCA?CAAGGCATTG?CTTCCTTGCT?GTGTGCTCAT?GGTCCTGATG?1560
1561?TTGAGTGGAG?AATATGTACC?ATCTGGGAAG?CTGCCTATGG?TTTGTTACCT?CTGAATTCAT?1620
1621?CAGCAGTTGA?TTTACCCGAA?ATCGTTGTAG?CTGCTCCGCT?TCAGCCACCT?ACTTTGTCAT?1680
1681?GGAGCCTATA?CTTGCCACTG?TTGAAAGTAT?TTGAGTATCT?ACCTCGTGGA?AGTCCATCTG?1740
1741?AAGCATGCCT?TATGAGAATA?TTTGTGGCAA?CAGTTGAAGC?TATACTCAGA?AGAACTTTCC?1800
1801?CTTCGGAAAC?CTCTGAATCA?TCTAAAAGAC?CAAGAAGTCA?ATCCAAGAAC?CTTGCTGTTG?1860
1861?CTGAACTCCG?TACGATGATA?CATTCACTCT?TTGTTGAATC?ATGTGCTTCA?ATGAACCTTG?1920
1921?CTTCCCGGTT?GTTGTTTGTA?GTATTAACTG?TTTGCGTCAG?TCATCAAGCT?TTGCCAGGGG?1980
1981?GCAGCAAAAG?ACCAACTGGT?AGTGAAAACC?ATTCTTCTGA?GGAGGCCACT?GAGGACCCAA?2040
2041?GATTAACCAA?TGGAAGAAAT?AGGGTCAAGA?AGAAACAAGG?GCCTGTTGGT?ACATTTGACT?2100
2101?CGTATGTGCT?GGCCGCTGTT?TGTGCCTTAT?CTTGCGAGCT?TCAGCTGTTC?CCTATCCTTT?2160
2161?GCAAGAGCGC?AACAAACTCA?AATGTAAAAG?ACTCTATAAA?GATCCTGAAG?CCTGGAAAAA?2220
2221?ACAATGGGAT?CAGTAATGAG?CTACAGAACA?GCATTAGCTC?AGCAATTCTC?CATACTCGTA?2280
2281?GAATTCTTGG?CATCCTGGAA?GCTCTTTTCT?CCTTGAAGCC?ATCATCAGTT?GGTACCTCCT?2340
2341?GGAACTATAG?TTCAAATGAG?ATAGTTGCAG?CGGCTATGGT?TGCCGCTCAT?GCTTCTGAGT?2400
2401?TATTTCGCCG?GTCGAAGGCA?TGCCTAAATG?CACTATCTTC?CCTGAAGCGA?TGTAAGTGGG?2460
2461?ATGCTGAGAT?TTCTACCAGG?GCATCATCCC?TCTACCATTT?GATCGACTTG?CATGGTAAAA?2520
2521?CTGTGTCCTC?CATCGTGAAC?AAAGCTGAGC?CTCTAGAAGC?TCACCTGACT?TTTACATCAG?2580
2581?TAAAGAGAGA?TGATGAACAA?CACATTGAGG?AAAATGGCAC?CAGCTCATCG?GGTAGTGGCA?2640
2641?ACTTGGAAAA?GAAGAATGGT?TCAGCCTCAC?ACATGAAAAA?TGGTTTGTCA?AGACCACTCT?2700
2701?TGAAATGCTC?AGAAGAGGCT?AGGCGAAATG?GTAATGTTGC?AAGTACATCC?GGGAAAGTTC?2760
2761?CTGCAACTTT?ACAGGCTGAA?GCTTCTGATT?TGGCGAACTT?CCTTACCATG?GATAGGAATG?2820
2821?GCGGTTATCG?AGGCTCTCAG?ACTCTCCTAA?GATCTGTTAT?CTCAGAAAAA?CAGGAACTAA?2880
2881?GCTTCTCTGT?TGTCTCATTG?CTCTGGCATA?AGCTTATTGC?ATCTCCTGAA?ACGCAGATGT?2940
2941?CTGCAGAAAG?TACATCAGCT?CATCAAGGTT?GGAGAAAGGT?TGTAGATGCT?CTTTGTGATG?3000
3001?TTGTTTCAGC?CTCACCAGCC?AAGGCTTCAA?CTGCTATTGT?TCTGCAGGCT?GAGAAGGACC?3060
3061?TACAGCCCTG?GATTGCTCGA?GATGATGAGG?AAGGTCAGAA?GATGTGGAGA?GTCAACCAGC?3120
3121?GAATAGTTAA?ACTGATAGCT?GAGCTTATGA?GGAACCATGA?TAGCCCAGAA?GCATTGATAA?3180
3181?TTCTTGCTAG?TGCTTCAGAC?CTTCTGCTTC?GTGCTACAGA?TGGGATGCTT?GTTGATGGTG?3240
3241?AAGCTTGTAC?CTTGCCTCAG?TTAGAGCTCC?TGGAAGTAAC?TGCTAGAGCT?ATTCATCTCA?3300
3301?TCGTTGAATG?GGGAGACCCA?GGTGTAGCAG?TTGCTGATGG?CCTCTCAAAT?CTGTTAAAGT?3360
3361?GCCGTCTATC?GCCTACCATC?CGCTGCCTCT?CCCACGCTAG?CGCACACGTA?CGTGCGCTCA?3420
3421?GCATGTCCGT?CCTCCGTGAC?ATCTTGAACA?GTGGACCACT?AGGTTCCACT?AAGATCATTC?3480
3481?AAGGCGAGCA?ACGGAATGGC?ATCCAAAGCC?CGACCTACCA?ATGCGCGGCA?GCAAACACGG?3540
3541?TGAACTGGCA?AGCGGATGTC?GAGAGATGTA?TAGACTGGGA?AGCCCGCAGC?CGCCGCGCCA?3600
3601?CCGGGATGAC?CCTCGCCTTC?CTCACCGCTG?CGGCTAACGA?GCTCGGCTGC?CCCCTTCCTT?3660
3661?GCTGACAAGG?CCATATTTGG?AGCTGACAAT?CAGCGACACT?TGACAGTTGG?CGCGAGCAGT?3720
3721?TGCTGCATGG?TCAGCGAGCA?GGATGGCTAA?TTAATCCCTT?GCTCAAGGAT?GGCTTCCCAG?3780
3781?TCTGCCCTGC?CCCCATGATG?TGATTCAAAA?CTGATGTATA?TTAGCTGATG?TTCCCAATCA?3840
3841?TACGGAGCTT?TGCTCCCCCG?GTGTGATTTT?AACTTGCAAT?CTGACGTTAG?ATGTTCAAGC?3900
3901?ATATTGAAAC?TGCTTGTGCT?GTAACTTGTA?TTCATGATGT?AACCGAAAGA?TGTCCGCTAT?3960
3961?GGTAAATGAA?GACATGTCAT?TTTTCGTTCT?CCAAAAAAAA?AAAAAAAAAA?AA 4012
2. the function of a grow wheat TaGI1 gene according to claim 1 is characterized in that this gene overexpression in Arabidopis thaliana, can promote the transgenic arabidopsis early flowering.
CN 200410035916 2004-10-13 2004-10-13 Wheat TaGI1 gene and its cloning and use Expired - Fee Related CN1291020C (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102220347A (en) * 2011-04-14 2011-10-19 山东农业大学 Wheat TaTOC1 genes, cloning method and use thereof
CN101376673B (en) * 2007-08-29 2013-11-20 中国科学院上海生命科学研究院 Rice fat synthesis related gene and use thereof
CN109402141A (en) * 2015-06-04 2019-03-01 山东农业大学 The application of wheat male sterility gene WMS and its anther specific promoter

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101376673B (en) * 2007-08-29 2013-11-20 中国科学院上海生命科学研究院 Rice fat synthesis related gene and use thereof
CN102220347A (en) * 2011-04-14 2011-10-19 山东农业大学 Wheat TaTOC1 genes, cloning method and use thereof
CN102220347B (en) * 2011-04-14 2013-07-03 山东农业大学 Wheat TaTOC1 genes, cloning method and use thereof
CN109402141A (en) * 2015-06-04 2019-03-01 山东农业大学 The application of wheat male sterility gene WMS and its anther specific promoter

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