CN1647651A - Natural preservative and its preparing method - Google Patents
Natural preservative and its preparing method Download PDFInfo
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- CN1647651A CN1647651A CN 200410001143 CN200410001143A CN1647651A CN 1647651 A CN1647651 A CN 1647651A CN 200410001143 CN200410001143 CN 200410001143 CN 200410001143 A CN200410001143 A CN 200410001143A CN 1647651 A CN1647651 A CN 1647651A
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Abstract
The present invention relates to biological preservative with propolis, and is a kind of natural biological preservative with emulsified propolis after superfine treatment as main components and its preparation process. Propolis is crushed into powder, dissolved in medium, dissolved with edible surfactant solution and pumped into deionized water while stirring, and after maturing and filtering, nanometer level emulsified liquid as natural biological preservative is prepared. The natural biological preservative may be used for preservation of food, medicine and biological preparation, and has high preservative effect.
Description
FIELD OF THE INVENTION
The present invention relates to a kind of is the biological preservative and preparation method thereof of main component with the propolis, and particularly a kind of propolis through ultra fine is the natural biological antiseptic agent of main component.
Background technology
The basis of propolis is tree face cream compound, polyphenols, polysaccharose substance etc.Propolis is the material that honeybee is used for protecting, resisting damage by disease and insect and pathogenic microorganism, also is that repairing lair and interior environment disinfected sterilization get a kind of particular matter.Propolis can be prepared into antibiotic anticorrosion, the antistaling agent of wide spectrum, can suppress the invasion and attack of bacterium, mould etc. effectively, regulates by the metabolism of fresh-keeping thing, prevents moisture loss, prolongs anticorrosion, freshness date.
The propolis preservative of traditional handicraft preparation is to the influence of the color and luster and the local flavor of anticorrosion object, and range of application is very narrow.
It all is to adopt propolis is dissolved in the alcohol to be added drop-wise to then in the aqueous solution that common process prepares propolis emulsification fluid, and the emulsion that obtains not only particle is thick, and antiseptic effect is far below alcoholic solution, and emulsification times is long, quantitatively property is poor, can't large-scale industrial production, and poor repeatability between batch.
The propolis preservative of the present invention's preparation is not only carried the antiseptic effect (with respect to common emulsion) of emulsion significantly, and can be made the color and luster of anticorrosion object and local flavor obtain good modification.
Summary of the invention
The objective of the invention is, a kind of is the biological preservative and preparation method thereof of main component with the propolis, and particularly a kind of propolis emulsification fluid through ultra fine is the natural biological antiseptic agent of main component.Adopt the propolis preservative technology of this method preparation simple, preparation time is short, industrialization easily, and the emulsion particle is little, and favorable anti-corrosion effect is widely used in the anticorrosion of food, medicine, biochemical product.
A kind of is the biodegradable preparation method of main component with the propolis, it may further comprise the steps: propolis is ground into powder, after medium dissolves, add the edible surface activator solution, dissolving quantitatively joins with pump in the deionized water of stirring fully, stir ripe down then, filter, obtain nano level natural biological antiseptic agent propolis emulsification fluid, can be applied to the anticorrosion of food, medicine, biochemical product.It is characterized in that, adopt the edible surface activating agent to make emulsifier, add, obtain the emulsion of average grain diameter less than 250 nanometers with the pump fast quantification.
A kind of preferred version, wherein said medium are the 70%-100% edible ethanol, and wherein the best is 95% ethanol.
A kind of preferred version, the quality (gram) of wherein said medium consumption (volume, milliliter) and propolis is than being 2.5-1000, and preferable range is 3.3-20, and the best is 10.
A kind of preferred version, wherein said solution temperature are less than 100 ℃, and optimum condition is a room temperature.
A kind of preferred version, wherein said edible surface activating agent comprises Tween-20, Tween-40, Tween-60, Tween-80, Si Pan-20, Si Pan-40, Si Pan-60, Si Pan-80, dolomol and combination thereof etc., optimum condition is a Tween-80, addition (quality, gram) be propolis (quality, gram) 1/100-1/2, preferred version is 1/10.
A kind of preferred version, wherein said preparation temperature scope is 0-90 ℃, and preferred version is 10-40 ℃, and optimal case is a room temperature.
A kind of preferred version, wherein said alr mode are that high shear rotor mixing speed is 100-10000 rev/min, and preferred version is 3000-5000 rev/min; It is 100-3000 rev/min that the oar formula stirs mixing speed, and preferred version is 800-1000 rev/min; Or use ultrasonic oscillation.
A kind of preferred version, wherein said flow are that whole propolis solution added at 0.1-60 minute, and preferred version is 0.5-2 minute, and preferred plan is 1 minute.
A kind of preferred version, wherein said maturation time are 0-30 minute, and preferred version is 0.1-2 minute, and preferred plan is 0.5 minute.
The propolis of indication of the present invention can be the solid-liquid two-phase not bee glue purifying, bee glue purifying, contain other products of propolis.
The medium of indication of the present invention comprises but not only refers to ethanol, sodium hydrate aqueous solution.
The edible surface activating agent of indication of the present invention comprises but not only refers to Tween-20, Tween-40, Tween-60, Tween-80, Si Pan-20, Si Pan-40, Si Pan-60, Si Pan-80, dolomol and combination thereof etc.
The stirring of indication of the present invention comprises but not only refers to high shear rotor emulsifier, dasher, hypergravity emulsification pump, artificial stirring, ultrasonic stirring etc.
The pump fast quantification of indication of the present invention adds, and comprises but refers to that not only peristaltic pump, gear pump, centrifugal pump, screw pump, plunger pump quantitatively add.
The present invention adopts surfactant to join in the medium solution, quantitatively adds with pump, adds accurately, and good reproducibility is easy to suitability for industrialized production, and the emulsion particle is little, and antiseptic effect is obvious.
Embodiment
The present invention will be further described below by embodiment.
Embodiment 1
Ultra-fineization propolis preservative process is: 100g is ground into powder with propolis, join in 95% the 1000ml edible ethanol, wait to dissolve the 100ml Tween 80 aqueous solution of back adding 10%, in container, add the 9kg deionized water, stir with the high shear rotor, 3000 rev/mins of mixing speeds, the temperature room temperature.At 1 minute propolis solution is all added, ripe 0.5 minute, filter then, obtain propolis emulsification fluid, the average upright footpath that records emulsion with the dynamic light scattering particle size distribution instrument is 195 nanometers.
Embodiment 2
Ultra-fineization propolis preservative process is: 5g is ground into powder with propolis, joins in 95% the 50ml edible ethanol, waits to dissolve the back and adds 10% the 5ml Tween 80 aqueous solution, in container, add the 9.95kg deionized water, stir 2500 rev/mins of mixing speeds, temperature room temperature with the high shear rotor.At 1 minute propolis solution is all added, ripe 1.0 minutes, filter then, obtain propolis emulsification fluid, the average upright footpath that records emulsion with the dynamic light scattering particle size distribution instrument is 128 nanometers.
Embodiment 3
Ultra-fineization propolis preservative process is: 1 5g is ground into powder with propolis, joins in 70% the 250ml edible ethanol, waits to dissolve the back and adds 10% 10ml Si Pan-60 aqueous solution, in container, add the 8kg deionized water, stir 800 rev/mins of mixing speeds, temperature room temperature with the oar formula.At 2 minutes propolis solution is all added, ripe 1.5 minutes, filter then, obtain propolis emulsification fluid, the average upright footpath that records emulsion with the dynamic light scattering particle size distribution instrument is 227 nanometers.
Embodiment 4
Ultra-fineization propolis preservative process is: 50g is ground into powder with propolis, join in 80% the 500ml edible ethanol, wait to dissolve the 50ml polysorbate40 aqueous solution of back adding 10%, in container, add the 9.4kg deionized water, stir with the high shear rotor, 4000 rev/mins of mixing speeds, the temperature room temperature.At 1 minute propolis solution is all added, ripe 0.5 minute, filter then, obtain propolis emulsification fluid, the average upright footpath that records emulsion with the dynamic light scattering particle size distribution instrument is 156 nanometers.
Claims (6)
1. one kind is biological preservative of main component and preparation method thereof with the propolis, and particularly a kind of propolis through ultra fine is the natural biological antiseptic agent of main component.This method is that propolis is ground into powder, after medium dissolves, add the edible surface activator solution, dissolving fully, quantitatively join with pump in the deionized water of high shear stirring, stir maturation then, filter, obtain nano level emulsion, can be applied to the anticorrosion of food, medicine, biochemical product.It is characterized in that, adopt the edible surface activating agent to make emulsifier, add, obtain the emulsion of average grain diameter less than 200 nanometers with the pump fast quantification.Adopt the propolis preservative technology of this method preparation simple, industrialization easily, the emulsion particle is little, and favorable anti-corrosion effect is widely used in the anticorrosion of food, medicine, biochemical product.
2. biological preservative according to claim 1 and preparation method thereof is characterized in that, described medium is the edible ethanol of 70-100%.Wherein the best is 95% ethanol.The mass ratio of medium consumption and propolis is 2.5-1000, and preferable range is 3.3-20, and solution temperature is less than 100 ℃, and optimum condition is a room temperature.
3. biological preservative according to claim 1 and preparation method thereof, described edible surface activating agent comprises Tween-20, Tween-40 Tween-60 Tween-80, Si Pan-20 Si Pan-40 Si Pan-60 Si Pan-80, dolomol and combination etc. thereof, optimum condition is a Tween-80, and addition is the 1/100-1/2 of propolis, and preferred version is 1/10.
4. biological preservative according to claim 1 and preparation method thereof, its described preparation temperature scope is 0-90 ℃, and preferred version is 10-40 ℃, and optimal case is a room temperature.Alr mode is that high shear rotor mixing speed is 100-10000 rev/min, and preferred version is 3000-5000 rev/min; It is 100-3000 rev/min that the oar formula stirs mixing speed, and preferred version is 800-1000 rev/min; Use ultrasonic oscillation.
5. biological preservative according to claim 1 and preparation method thereof, its described flow are that whole propolis solution added at 0.1-60 minute, and preferred version is 0.5-2 minute, and preferred plan is 1 minute.
6. biological preservative according to claim 1 and preparation method thereof, its described maturation time is 0-30 minute, and preferred version is 0.1-2 minute, and preferred plan is 0.5 minute.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410001143 CN1647651A (en) | 2004-01-30 | 2004-01-30 | Natural preservative and its preparing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410001143 CN1647651A (en) | 2004-01-30 | 2004-01-30 | Natural preservative and its preparing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1647651A true CN1647651A (en) | 2005-08-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200410001143 Pending CN1647651A (en) | 2004-01-30 | 2004-01-30 | Natural preservative and its preparing method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009111848A1 (en) * | 2008-03-10 | 2009-09-17 | Universidade De São Paulo - Usp | Extracts of baccharis sp. or green propolis for use as sanitizer |
| CN101011419B (en) * | 2007-02-06 | 2011-01-19 | 傅金龙 | Propolis preparation and its preparation method |
| CN102715624A (en) * | 2012-06-07 | 2012-10-10 | 南通市海顺化工有限公司 | Natural preservative |
| CN105778597A (en) * | 2016-05-19 | 2016-07-20 | 陆玉如 | Natural preservative for insulation coating |
-
2004
- 2004-01-30 CN CN 200410001143 patent/CN1647651A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011419B (en) * | 2007-02-06 | 2011-01-19 | 傅金龙 | Propolis preparation and its preparation method |
| WO2009111848A1 (en) * | 2008-03-10 | 2009-09-17 | Universidade De São Paulo - Usp | Extracts of baccharis sp. or green propolis for use as sanitizer |
| CN102715624A (en) * | 2012-06-07 | 2012-10-10 | 南通市海顺化工有限公司 | Natural preservative |
| CN105778597A (en) * | 2016-05-19 | 2016-07-20 | 陆玉如 | Natural preservative for insulation coating |
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