CN1644112A - Production of pure soya-bean lactacidase fermenting beverage - Google Patents

Production of pure soya-bean lactacidase fermenting beverage Download PDF

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CN1644112A
CN1644112A CNA2005100382986A CN200510038298A CN1644112A CN 1644112 A CN1644112 A CN 1644112A CN A2005100382986 A CNA2005100382986 A CN A2005100382986A CN 200510038298 A CN200510038298 A CN 200510038298A CN 1644112 A CN1644112 A CN 1644112A
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soya
nutrient solution
bean milk
bean
preparation
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CN1317985C (en
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周惠明
钱海峰
赵宇星
孙欣
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Jiangnan University
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Jiangnan University
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Abstract

A fermented lactobacillus beverage is prepared from soybean through preparing soybean milk, inoculating thermophilic streptococcus and lactobacillus, fermenting, dispensing, homogenizing and sterilizing. Its advantages are agreeable taste, not containing cholesterine and lactose, and high nutritive value.

Description

A kind of preparation method of pure soya-bean lactacidase fermenting beverage
Technical field
The present invention relates to a kind of is the lactacidase fermenting beverage of raw material with pure soybean, relate in particular to the pure soy yogurt that uses special streptococcus thermophilus of taming of warp and bulgaria lactobacillus fermentation to obtain, again through allotment, homogeneous, technologies such as sterilization make, and soy yogurt beverage with long preservation period and preparation method thereof under the normal temperature.
Background technology
Acidified milk has higher nutritive value.At present modal is cultured milk, but this be that its cholesterol level of yogurt of base-material is higher with the animal breast.Because soy protein content height, and in phytoprotein, have soybean protein only and just contain necessary each seed amino acid of human body fully, the soybean price is lower in addition, so the someone utilizes big soya-bean milk to produce soy yogurt at present, by the lactobacillus-fermented soya-bean milk, can remove the intrinsic beany flavor of soybean, reduce the oligosaccharides component content that causes flatulence easily, and can produce organic acid, and suppress the growth of enteron aisle saprophytic bacteria, have anticancer, improve the human body functions of intestines and stomach, improve effects such as immunity function and reduction serum cholesterol,, be subjected to liking of consumer day by day so prepare lactic acid bacteria soya-bean milk drink as fermented bacterium with lactic acid bacteria.
Before the present invention, there have been relevant report and product.But soya-bean milk is significantly different with the yeasting of cow's milk, lactic acid bacteria grows relatively poor in pure soya-bean milk, produce subacidity, so existed the fermentation substrate of report or product to be generally the mixture of soya-bean milk and cow's milk or milk powder, cow's milk and milk powder contain cholesterol and lactose, long-term drinking can make the human cholesterol content raise, and especially is not suitable for the elderly's long-term drinking.The present invention tames especially by to key factor---the bacterial classification in the fermentation, makes its fermentation system that adapts to pure soya-bean milk, produces not contain cow's milk but the soy yogurt of quality better.
Summary of the invention
The objective of the invention is to seek a kind of preparation method of pure soya-bean lactacidase fermenting beverage, is that raw material makes soya-bean milk with pure soybean, by inoculation fermentation, and allotment, homogeneous, sterilization makes pure soya-bean lactacidase fermenting beverage.Wherein the bacterial classification of fermentation employing is streptococcus thermophilus and lactobacillus bulgaricus, has passed through special domestication process, is fit to the fermentation system of pure soya-bean milk.
The fermentation base-material of soy yogurt is pure soya-bean milk, sucrose and glucose among the present invention, and its solid content is 14~16%, also comprises stabilizing agent, emulsifying agent and essence in its raw material.According to the beverage that the present invention finally makes, tissue is fine and smooth, and mouthfeel is good, and is sour-sweet tasty and refreshing, and pure bean-fragrance is more reasonable than cultured milk nutrition.
The manufacture craft of pure soya-bean milk fermentation drink of the present invention is as follows:
The preparation of a, soya-bean milk fermentation base-material: soybean is after the peeling machine decortication, with the 0.25%NaHCO of 3~4 times of quality 3Solution soaks, clear water is cleaned, with 90~95 ℃ of hot water blanchings 5~8 minutes, be 1: 6~1: 8 hot water defibrination at 90~95 ℃ by material-water ratio again, with obtaining soya-bean milk behind 120~150 order filter cloth filter and remove residues, add the sugar that accounts for soya-bean milk weight 6~8%, sterilized 10~15 minutes for 115~120 ℃, the fermentation base-material is made in cooling;
The preparation of b, strain domestication nutrient solution: part fermentation base-material and the skimmed milk of getting process a are 0: 10 by volume, 2: 8,4: 6,6: 4,8: 2, be mixed into 6 kind at 10: 0, be loaded on respectively then in the test tube separately, 115~120 ℃ of sterilizations 10~15 minutes, cooling obtains the strain domestication nutrient solution, and is designated as 1. number nutrient solution, 2. number nutrient solution, 3. number nutrient solution, 4. number nutrient solution, 5. number nutrient solution and 6. number nutrient solution successively;
The domestication of c, bacterial classification: 1. streptococcus thermophilus is inoculated in number nutrient solution, addition is 3~6% of a nutrient solution volume, under 37~45 ℃ of temperature conditions, cultivate, treat to be inoculated into after nutrient solution solidifies 2. in number nutrient solution, be inoculated into 3. number nutrient solution, 4. number nutrient solution, 5. number nutrient solution and 6. number nutrient solution more successively, each addition is identical with cultivation temperature, progressively improves soya-bean milk concentration, obtains being fit to the domestication streptococcus thermophilus of soya-bean milk fermentation system at last; Utilization obtains taming lactobacillus bulgaricus with method;
D, the preparation of producing leavening: the part of getting process a fermentation base-material, 115~120 ℃ of sterilizations are after 10~15 minutes, be cooled to 37~45 ℃, the domestication streptococcus thermophilus and the domestication lactobacillus bulgaricus that add its volume 3~6% respectively, under 37~45 ℃ of conditions, cultivated 6~8 hours, and obtained producing leavening;
E, fermentation: in the fermentation base-material for preparing by process a, add the production leavening that obtains by process d, addition is 3~6% of fermentation base-material cumulative volume, 37~45 ℃ of bottom fermentations 6~8 hours, then 0~4 ℃ down slaking overnight once obtain fermented soybean milk;
F, allotment homogeneous: with emulsifying agent, stabilizing agent, essence and sucrose are dissolved in 40~50 ℃ the warm water, and the fermented soybean milk that obtains with process e mixes again, and again 60~70 ℃ of temperature, homogeneous handles twice under pressure 20~30Mpa, obtains soy yogurt;
G, sterilization packaging: with above-mentioned soy yogurt 80~90 ℃ of down sterilization cans in 30~45 minutes, overnight more once with the same terms sterilization; Perhaps with above-mentioned soy yogurt under 137~140 ℃ through 4~5 seconds ultra high temperature short time sterilization, cooling back packing.
In the preparation process of soya-bean milk fermentation base-material, the sugar of adding is sucrose or/and glucose, if when being the compound of sucrose and glucose, both ratios are 1: 1.
In the process of preparation leavening, adding the domestication streptococcus thermophilus is 1: 1~1: 2 with the ratio of taming lactobacillus bulgaricus.
In the allotment homogenizing process, dissolved emulsifier, stabilizing agent, the amount of the warm water of essence and sucrose obtains 1~2 times of soya-bean milk for fermenting, and the emulsifying agent of adding is a sucrose ester, and adding proportion is 0.05~0.15% of a soya-bean milk weight; Stabilizing agent is propylene glycol alginate or sodium carboxymethylcellulose, adding proportion is 0.3~0.5% of a soya-bean milk weight, adding the sucrose ratio is 8~10% of soya-bean milk weight, and the essence of interpolation is water-soluble soymilk or fruit essence, and addition is 0.05~0.2% of a soya-bean milk weight.
Soak time can be grasped flexibly according to season in the process of preparation soya-bean milk fermentation base-material, adjusted in good time; Use NaHCO 3Solution soaks, and can eliminate the part beany flavor; By blanching and hot water defibrination can the passivation part ANFs, reduce the beany flavor that makes soya-bean milk.The solid content that makes base-material according to the present invention is 14~16%, and wherein sucrose of Jia Ruing and glucose account for 6~8%.All the other are demineralized water.
When the allotment homogeneous, emulsifying agent, stabilizing agent and essence mix with sugar earlier, again with 1~2 times of warm water dissolving of 40~50 ℃ that soya-bean milk is heavy, add the soya-bean milk of fermentation again, stir.
When sterilization packaging, can adopt vinyon Bottle ﹠ Can dress, then 80~90 ℃ of sterilizations 30~45 minutes down, overnight more once with the same terms sterilization; Perhaps the soy yogurt behind the homogeneous is carried out 4~5 seconds ultra high temperature short time sterilization under 137~140 ℃, the aseptic packaging of Tetra Pak flexible packaging box is adopted in the cooling back.
The relative prior art of the present invention, main inventive point is at pure soybean system, carry out suitable strain domestication and pure soybean system is fermented, the vigor maintenance of repeatedly taming bacterial classification is necessary, bacterial classification through domestication can long preservation, the preceding strain domestication process of each fermentation can omit after.Mostly need add an amount of cow's milk in fermentation system both at home and abroad, the strain domestication method is also not quite alike.The present invention describes for convenient, will tame number of times to be defined as 6 times, but the present invention claimed be a kind of utilization repeatedly acclimation method keep the vigor of bacterial classification, can be 6 times as for concrete domestication number of times, also can slightly do increase and decrease on this basis.
The lactacidase fermenting beverage that makes according to the present invention, tissue is fine and smooth, and sour-sweet tasty and refreshing, pure bean-fragrance does not contain cholesterol and lactose, has higher nutritive value.Can preserve more than 3 months at normal temperatures, help carrying and drinking when the way far away transportation of product and consumer go out.
A kind of is the physical and chemical index of lactacidase fermenting beverage after slaking of raw material with pure soybean:
Project Index
Fat (%) ????>1.5
Protein (%) ????>3
Non-fat solid (%) ????>10
Acidity (° T) ????>70
pH ????<4.5
Urease (mg/g) ????0
Trypsin inhibitor (TIU/ml) ????<500
Lactobacillus (cfu/mL) ????>1.5×10 8
A kind of index that with pure soybean is the lactacidase fermenting beverage of raw material at homogeneous sterilization back product
Project Index
The organoleptic indicator
Color and luster Even milky
Flavour and smell Sour and sweet palatability, good mouthfeel, pure bean-fragrance
Structural state Be the milkiness shape, tissue is fine and smooth, uniformity, no lamination, no bubble, no foreign matter
Physical and chemical index
Total solids content (%) >12
Protein (%) >1.0
The pH value <4.5
Acidity (° T) >40
Urase (mg/g) 0
Trypsin inhibitor (TIU/ml) <200
Centrifugation rate (%) <1.5
Centrifugal come-up layer (%) <1
Coefficient of stability R (%) >90
Microbiological indicator
Total plate count (individual/ml) <30
Coliform (individual/100ml) <3
Pathogenic bacteria (duodenum 12 road pathogenic bacteria and pathogenic coccus) Must not detect
The specific embodiment
Manufacture craft of the present invention is as follows:
The preparation of a, soya-bean milk fermentation base-material: soybean is after the peeling machine decortication, with the 0.25%NaHCO of 3~4 times of quality 3Solution soaks, clear water is cleaned, with 90~95 ℃ of hot water blanchings 5~8 minutes, be 1: 6~1: 8 hot water defibrination at 90~95 ℃ by material-water ratio again, with obtaining soya-bean milk behind 120~150 order filter cloth filter and remove residues, add the sugar that accounts for soya-bean milk weight 6~8%, sterilized 10~15 minutes for 115~120 ℃, the fermentation base-material is made in cooling, and sugar wherein can be for sucrose or/and glucose, if when being the compound of sucrose and glucose, both ratios are 1: 1;
The preparation of b, strain domestication nutrient solution: part fermentation base-material and the skimmed milk of getting process a are 0: 10 by volume, 2: 8,4: 6,6: 4,8: 2, be mixed into 6 kind at 10: 0, be loaded on respectively then in the test tube separately, 115~120 ℃ of sterilizations 10~15 minutes, cooling obtains the strain domestication nutrient solution, and is designated as 1. number nutrient solution, 2. number nutrient solution, 3. number nutrient solution, 4. number nutrient solution, 5. number nutrient solution and 6. number nutrient solution successively;
The domestication of c, bacterial classification: 1. streptococcus thermophilus is inoculated in number nutrient solution, addition is 3~6% of a nutrient solution volume, temperature is cultivated under 37~45 ℃ of conditions, treat to be inoculated into after nutrient solution solidifies 2. in number nutrient solution, be inoculated into 3. number nutrient solution, 4. number nutrient solution, 5. number nutrient solution and 6. number nutrient solution more successively, each addition is identical with temperature, progressively improves soya-bean milk concentration, obtains being fit to the domestication streptococcus thermophilus of soya-bean milk fermentation system at last; The lactobacillus bulgaricus that utilization obtains taming with method;
D, the preparation of producing leavening: the part of getting process a fermentation base-material, 115~120 ℃ of sterilizations were cooled to 37~45 ℃ after 10~15 minutes, the domestication streptococcus thermophilus and the domestication lactobacillus bulgaricus that add its volume 3~6% respectively, under 37~45 ℃ of conditions, cultivated 6~8 hours, obtain producing leavening, wherein, adding the domestication streptococcus thermophilus is 1: 1~1: 2 with the ratio of taming lactobacillus bulgaricus;
E, fermentation: in the fermentation base-material for preparing by process a, add the production leavening that obtains by process d, addition is 3~6% of fermentation base-material cumulative volume, 37~45 ℃ of bottom fermentations 6~8 hours, then 0~4 ℃ down slaking overnight once obtain fermented soybean milk;
F, allotment homogeneous: with emulsifying agent, stabilizing agent, essence and sucrose are dissolved in 40~50 ℃ the warm water, the fermented soybean milk that obtains with process e mixes again, 60~70 ℃ of temperature, homogeneous handles twice under pressure 20~30Mpa, obtains soy yogurt again, wherein, dissolved emulsifier, stabilizing agent, the amount of the warm water of essence and sucrose is 1~2 times of fermented soybean milk, the emulsifying agent that adds is a sucrose ester, and adding proportion is 0.05~0.15% of a fermented soybean milk weight; Stabilizing agent is propylene glycol alginate or sodium carboxymethylcellulose, adding proportion is 0.3~0.5% of a fermented soybean milk weight, adding the sucrose ratio is 8~10% of fermented soybean milk weight, and the essence of interpolation is water-soluble soymilk or fruit essence, and addition is 0.05~0.2% of a fermented soybean milk weight;
G, sterilization packaging: with above-mentioned soy yogurt 80~90 ℃ of down sterilization cans in 30~45 minutes, overnight more once with the same terms sterilization; Perhaps with above-mentioned soy yogurt under 137~140 ℃ through 4~5 seconds ultra high temperature short time sterilization, cooling back packing.
Lift two examples below
Example 1
10 kilograms of soybean after the screening and removing impurities of learning from else's experience, under the room temperature with 30 liter 0.25% NaHCO 3Solution soaks 10~16 hours (the concrete time is with deciding season), clean the back earlier with 95 ℃ of hot water blanchings 5 minutes, use 95 ℃ of hot water defibrinations again, slurry obtains pure soya-bean milk through 150 order filter and remove residues, adds 4.8 kilograms of sucrose and 1.6 kilograms of glucose again, add water to 80 liters, sterilized 10 minutes for 118 ℃, be cooled to 43 ℃, obtain the soya-bean milk fermentation base-material.
Get 1 and go up and state the culture medium that base-material and cow's milk are made into different proportion, the volume ratio of soya-bean milk and cow's milk was respectively 0: 10,2: 8,4: 6,6: 4,8: 2,10: 0, the test tube of packing into, every pipe 100ml is 118 ℃ of sterilizations 15 minutes down, be cooled to 43 ℃, volume ratio by 3% adds streptococcus thermophilus and lactobacillus bulgaricus respectively, cultivates curdled milk, obtains domesticated strain.
Get 3 liters of soya-bean milk fermentation base-materials, add the streptococcus thermophilus of 30ml domestication and the lactobacillus bulgaricus of 60ml domestication respectively, then this zymotic fluid is put into fermenting cellar, obtain producing leavening in 43 ℃ of fermentations 6 hours.
In 76 liters of soya-bean milk base-materials of remainder, add 2.3 liters and produce leavenings, then this zymotic fluid is put into fermenting cellar, then 4 ℃ of following slakings overnight 16 hours in 43 ℃ of fermentations 6 hours.
Get 12.8 kilograms of sucrose, sucrose ester 240 grams, sodium carboxymethylcellulose 960 grams, 120 gram soymilk essence mix, and add 160 kilograms of drinking water dissolvings, with the fermented soybean milk mixing and stirring, at 60 ℃, twice of the last homogeneous of 30MPa.
It is canned in the vinyon bottle that soy yogurt behind this homogeneous (is not less than 50 ℃) while hot, packing had the plastic bottle of soy yogurt to insert 80 ℃ of water dip sterilizations 30 minutes again, overnight after again the same terms sterilization once, but normal temperature was preserved 3 months.
Example 2
10 kilograms of soybean after the screening and removing impurities of learning from else's experience, under the room temperature with 30 liter 0.25% NaHCO 3Solution soaks 10~16 hours (the concrete time is with deciding season), clean the back earlier with 95 ℃ of hot water blanchings 5 minutes, use 95 ℃ of hot water defibrinations again, slurry obtains pure soya-bean milk through 150 order filter and remove residues, adds 4.8 kilograms of sucrose and 1.6 kilograms of glucose again, add water to 80 liters, sterilized 10 minutes for 118 ℃, be cooled to 43 ℃, obtain the soya-bean milk fermentation base-material.
Get 1 and go up and state the culture medium that base-material and cow's milk are made into different proportion, the volume ratio of soya-bean milk and cow's milk was respectively 0: 10,2: 8,4: 6,6: 4,8: 2,10: 0, the test tube of packing into, every pipe 100ml is 118 ℃ of sterilizations 15 minutes down, be cooled to 43 ℃, volume ratio by 3% adds streptococcus thermophilus and lactobacillus bulgaricus respectively, cultivates curdled milk, obtains domesticated strain.
Get 3 liters of soya-bean milk fermentation base-materials, add the streptococcus thermophilus of 45ml domestication and the lactobacillus bulgaricus of 45ml domestication respectively, then this zymotic fluid is put into fermenting cellar, obtain producing leavening in 37 ℃ of fermentations 10 hours.
In 76 liters of soya-bean milk base-materials of remainder, add 3 liters and produce leavenings, then this zymotic fluid is put into fermenting cellar, then 4 ℃ of following slakings overnight 12 hours in 37 ℃ of fermentations 10 hours.
12.8 kilograms of sucrose, sucrose ester 240 grams, sodium carboxymethylcellulose 960 grams, propylene glycol alginate 240 grams, 120 gram soymilk essence mix, and add 160 kilograms of drinking water dissolvings, with the fermented soybean milk mixing and stirring, at 60 ℃, twice of the last homogeneous of 30MPa.
Soy yogurt behind this homogeneous is carried out 4~5 seconds ultra high temperature short time sterilization under 137~140 ℃, the aseptic packaging of Tetra Pak flexible packaging box is adopted in the cooling back, but normal temperature was preserved 3 months.

Claims (4)

1, a kind of preparation method of pure soya-bean lactacidase fermenting beverage is characterized in that:
The preparation of a, soya-bean milk fermentation base-material: soybean is after the peeling machine decortication, with the 0.25%NaHCO of 3~4 times of quality 3Solution soaks, clear water is cleaned, with 90~95 ℃ of hot water blanchings 5~8 minutes, be 1: 6~1: 8 hot water defibrination at 90~95 ℃ by material-water ratio again, with obtaining soya-bean milk behind 120~150 order filter cloth filter and remove residues, add the sugar that accounts for soya-bean milk weight 6~8%, sterilized 10~15 minutes for 115~120 ℃, the fermentation base-material is made in cooling;
The preparation of b, strain domestication nutrient solution: part fermentation base-material and the skimmed milk of getting process a are 0: 10 by volume, 2: 8,4: 6,6: 4,8: 2, be mixed into 6 kind at 10: 0, be loaded on respectively then in the test tube separately, 115~120 ℃ of sterilizations 10~15 minutes, cooling obtains the strain domestication nutrient solution, and is designated as 1. number nutrient solution, 2. number nutrient solution, 3. number nutrient solution, 4. number nutrient solution, 5. number nutrient solution and 6. number nutrient solution successively;
The domestication of c, bacterial classification: 1. streptococcus thermophilus is inoculated in number nutrient solution, addition is 3~6% of a nutrient solution volume, under 37~45 ℃ of temperature conditions, cultivate, treat to be inoculated into after nutrient solution solidifies 2. in number nutrient solution, be inoculated into 3. number nutrient solution, 4. number nutrient solution, 5. number nutrient solution and 6. number nutrient solution more successively, each addition is identical with cultivation temperature, progressively improves soya-bean milk concentration, obtains being fit to the domestication streptococcus thermophilus of soya-bean milk fermentation system at last; Utilization obtains taming lactobacillus bulgaricus with method;
D, the preparation of producing leavening: the part of getting process a fermentation base-material, 115~120 ℃ of sterilizations are after 10~15 minutes, be cooled to 37~45 ℃, the domestication streptococcus thermophilus and the domestication lactobacillus bulgaricus that add its volume 3~6% respectively, under 37~45 ℃ of conditions, cultivated 6~8 hours, and obtained producing leavening;
E, fermentation: in the fermentation base-material for preparing by process a, add the production leavening that obtains by process d, addition is 3~6% of fermentation base-material cumulative volume, 37~45 ℃ of bottom fermentations 6~8 hours, then 0~4 ℃ down slaking overnight once obtain fermented soybean milk;
F, allotment homogeneous: with emulsifying agent, stabilizing agent, essence and sucrose are dissolved in 40~50 ℃ the warm water, and the fermented soybean milk that obtains with process e mixes, and then 60~70 ℃ of temperature, homogeneous handles twice under pressure 20~30Mpa, obtains soy yogurt;
G, sterilization packaging: the soy yogurt that process f is obtained is 80~90 ℃ of sterilizations 30~45 minutes down, can, overnight more once with the same terms sterilization; Perhaps with above-mentioned soy yogurt under 137~140 ℃ through 4~5 seconds ultra high temperature short time sterilization, cooling back packing.
2, the preparation method of pure soya-bean lactacidase fermenting beverage according to claim 1, it is characterized in that: in the preparation process of soya-bean milk fermentation base-material, the sugar that adds is sucrose or/and glucose, if when being the compound of sucrose and glucose, both ratios are 1: 1.
3, the preparation method of pure soya-bean lactacidase fermenting beverage according to claim 1 is characterized in that: in the process of preparation leavening, adding the domestication streptococcus thermophilus is 1: 1~1: 2 with the ratio of taming lactobacillus bulgaricus.
4, the preparation method of pure soya-bean lactacidase fermenting beverage according to claim 1, it is characterized in that: in the allotment homogenizing process, dissolved emulsifier, stabilizing agent, the amount of the warm water of essence and sucrose obtains 1~2 times of soya-bean milk for fermentation, the emulsifying agent that adds is a sucrose ester, and adding proportion is 0.05~0.15% of a soya-bean milk weight; Stabilizing agent is propylene glycol alginate or sodium carboxymethylcellulose, adding proportion is 0.3~0.5% of a soya-bean milk weight, adding the sucrose ratio is 8~10% of soya-bean milk weight, and the essence of interpolation is water-soluble soymilk or fruit essence, and addition is 0.05~0.2% of a soya-bean milk weight.
CNB2005100382986A 2005-01-26 2005-01-26 Production of pure soya-bean lactacidase fermenting beverage Expired - Fee Related CN1317985C (en)

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