CN1632114A - Process for producing high active pancreatin - Google Patents
Process for producing high active pancreatin Download PDFInfo
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- CN1632114A CN1632114A CN 200410081389 CN200410081389A CN1632114A CN 1632114 A CN1632114 A CN 1632114A CN 200410081389 CN200410081389 CN 200410081389 CN 200410081389 A CN200410081389 A CN 200410081389A CN 1632114 A CN1632114 A CN 1632114A
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Abstract
The invention relates to a new technique to prepare high activity pancreatin grind refrigerated or fresh pancreas of pig to be plasm and activate, adjust the PH value to be 6.0-8.0, add enzyme deposit protector; agitate it completely, deposit for 30-120min in 2-8deg.C, then add into little precipitant accelerator, agitate completely, continue to deposit for 3-7h in 2-8deg.C, centrifuge, accumulate the deposits, make the product after degreasing and drying. Using the technique to prepare pancreatin, has good safety, simple operation, short productive period, high productivity, high activity of enzyme, low energy cost, and needs no other special equipment.
Description
What technical field the present invention relates to is a kind of novel process that is used to prepare high vigor pancreatin, particularly is exactly to carry out sedimentary method with PEG (polyoxyethylene glycol) to activating the pig pancreas.
The background technology pancreatin is the digestants that various countries' pharmacopeia is recorded, it is the mixture of the plurality of enzymes from animal pancreas, extracted, main component is trypsinase, lipase and amylase, and it can be used for treating the gastricism that maldigestion, poor appetite and liver, pancreas illness cause.Maldigestion is human common disease, frequently-occurring disease, and especially the elderly's digestive functions weakens, and becomes to follow lifelong chronic illness.For dyspeptic treatment, reasonable way be exactly give can digest food in the various enzymes of protein, starch, fat, help human gastrointestinal tract to finish the digestion task, assist the conditioning health to increase the absorption of food nutrition.As the digestive ferment complementary medicine, high-quality pancreatin is widespread use abroad.In addition, owing to contain the various active material in the pancreatin, can be used as basic material and therefrom extract multiple biochemical drug, as pancreokinin proenzyme, elastoser, Asparaginase, trypsinase, Chymotrypsin, carboxypeptidase or the like.
Whether pancreatin has the characteristic of the enzyme of natural ratio in the pancreas, it is the key factor that influences the pancreatin quality, and three enzymes (trypsinase, lipase and amylase) content ratio is widely different in the pancreatin product of various processes preparation, therefore USP24 has clear and definite regulation to the content of three enzymes in the high standard pancreatin, and 2000 editions CP also have corresponding requirement to three enzyme content of pancreatin.
What the traditional technology of the medicinal pancreatin of production of bibliographical information generally adopted at present is the method for organic solvent deposit.For example, the optimization technology of from Pancreas Sus domestica, extracting pancreatin of report in " Medical University Of Chongqing's journal " 1995 (4), 305, the precipitation agent that uses in the technical process is an ethanol, three enzyme activities are respectively 4 in the product, 200U/g, 3,600U/g, 5,000U/g, yield 10～13%.The extraction agent that uses in the high vigor pancreatin production technique of " Chinese biochemical drug magazine " 1994 (3), 190 reports is the ethanol of lower concentration, and precipitation agent is the cold ethanol of high density, and three enzyme activities are respectively 4,500U/g, 3,500U/g, 7,000U/g, the pancreatin total recovery is 7.9～10.8%.Qianhong Biochemical Pharmaceutical Co., Ltd., Changzhou is at " Chinese biochemical drug magazine " 1993 (2), the precipitation agent that the pancreatin technology of report is used on 40 is an ethanol, three enzyme activities ratio is that (trypsinase: steapsase: pancreatic amylase), yield was 9～11% in 1: 16: 30 in the pancreatin product that obtains.Tsing-Hua University is at " Chinese biochemical drug magazine " 1994 (4), the high vigor pancreatin preparation technology of report uses in 283 extraction agent and precipitation agent all are ethanol, press dry product and calculate, three enzyme activities of every gram pancreas enzyme powder are respectively 281,000U, 18,300U, 37,400U, wherein lipase is relative with diastatic vigor on the low side, and yield is 10.47%.The Changsha biochemical-pharmaceutical factory is at " Qinghai medical magazine " 2000 (4), the precipitation agent that the pancreatin new preparation process of report uses on 56 is an acetone, the enzyme activity of every gram pancreas enzyme powder on average contains trypsinase 211,355U, lipase 9,957U, amylase 22,141U, wherein lipase is still relative with diastatic content on the low side, yield average out to 12.8%.In sum, all used a large amount of organic solvents in these technologies, because the inflammableness of organic reagent and to workman's chronic toxicity makes to have great potential safety hazard in the production; And because ethanol and acetone may make protein generation sex change, in the production process enzyme activity loss more, especially the loss of lipase and diastatic vigor is serious, causes three enzyme ratios unreasonable, yield is also unsatisfactory.
The comprehensive above operational path of summary of the invention the present invention proposes and a kind ofly precipitates activated pig pancreas with enzyme precipitation protective material PEG and starch the concrete grammar for preparing pancreatin.It is good, easy and simple to handle, with short production cycle that this method has a security, the yield height, and three enzyme activity height, energy consumption is low and do not need to increase characteristics such as special instruments and equipment, and application value is arranged.
Pancreatin new preparation process of the present invention is to precipitate activating the pig pancreas with PEG, to freeze pig pancreas or fresh pig pancreas and rub into the back activation of pancreas slurry, transferring pH is 6.0～8.0, adds PEG 10～50%, fully stir, precipitate 30～120min under 2～8 ℃ of conditions, add a spot of short precipitation agent then, fully stir, continue precipitation 3～7h under 2～8 ℃ of conditions, centrifugal, collecting precipitation gets the pancreatin finished product behind the degreaser drying.Supernatant liquor is for reclaiming PEG.
That aforesaid method of the present invention uses when activation pancreas slurry is precipitated the preparation pancreatin is enzyme precipitation protective material PEG.PEG is a kind of non-ionic water-soluble polymer, and as protein precipitant, PEG only just makes protein that sex change is arranged slightly under individual concentrations.Because PEG when dissolving heat radiation is low, precipitation usefulness is high and form characteristics such as sedimentary starting time is short, makes PEG become a useful reagent in proteinic component is separated.In the pancreatin new preparation process of the present invention, PEG has precipitating action preferably to pancreatin, and three enzyme activities obtain better protecting.Lot of experiment results confirms that the weight recovery of pancreatin can reach 14.3～18.0%, surpasses the highest level of bibliographical information; Three enzyme activities can reach 3 respectively in the pancreatin, 760U/g, and 28,000U/g, 53,000U/g reaches the Chinese Pharmacopoeia standard more than six times, and near the natural ratio in the pancreas.
The enzyme precipitation protective material PEG safety non-toxic that aforesaid method of the present invention uses.Ethanol, acetone etc. are inflammable, explosive, the usage quantity of toxic reagent owing to significantly reduced, and the security of production is improved greatly.
Aforesaid method of the present invention shortens the production cycle owing to be used in combination enzyme precipitation protective material and short precipitation agent greatly, and whole precipitation process at most only needs 8h.
The PEG that aforesaid method of the present invention uses can use for several times repeatedly, reclaims PEG from supernatant liquor, and yield on average can reach 95%.After PEG utilized 3 times continuously, the pancreatin rate of recovery still can reach 13.0%, three enzyme content did not have considerable change.The utilization again of PEG has reduced production cost to a certain extent.
Below by the embodiment of some examples, again foregoing of the present invention is described in further detail, but this should be interpreted as that the scope of the above-mentioned theme of the present invention only is confined to following example.All technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
Embodiment example 1:(1) gets freezing pig pancreas or fresh pig pancreas and rub into the back activation of pancreas slurry.(2) get activation pancreas slurry 10g (being equivalent to pig pancreas 4g), add water to 50mL, transferring pH is 7.0, adds PEG 15g, fully stir, precipitate 60min under 4 ℃ of conditions, add a spot of short precipitation agent then, fully stir, continue precipitation 5h under 4 ℃ of conditions, the centrifugal 10min of 3000rpm, collecting precipitation gets pancreatin finished product 0.67g behind the degreaser drying, weight recovery is 16.8%, trypsinase, lipase, diastatic content are respectively 3 in the product, 760U/g, 28,000U/g, 53,000U/g.Supernatant liquor is for reclaiming PEG.
Example 2:(1) gets freezing pig pancreas or fresh pig pancreas and rub into the back activation of pancreas slurry.(2) get activation pancreas slurry 10g (being equivalent to pig pancreas 4g), add water to 50mL, transferring pH is 6.5, adds PEG 15g, fully stir, precipitate 60min under 6 ℃ of conditions, add a spot of short precipitation agent then, fully stir, continue precipitation 6h under 6 ℃ of conditions, the centrifugal 10min of 3000rpm, collecting precipitation gets pancreatin finished product 0.63g behind the degreaser drying, weight recovery is 15.8%, trypsinase, lipase, diastatic content are respectively 3 in the product, 900U/g, 28,670U/g, 55,000U/g.Supernatant liquor is for reclaiming PEG.
Example 3:(1) gets freezing pig pancreas or fresh pig pancreas and rub into the back activation of pancreas slurry.(2) get activation pancreas slurry 50g (being equivalent to pig pancreas 20g), add water to 200mL, transferring pH is 7.5, adds PEG 50g, fully stir, precipitate 30min under 3 ℃ of conditions, add a spot of short precipitation agent then, fully stir, continue precipitation 7h under 3 ℃ of conditions, the centrifugal 10min of 3000rpm, collecting precipitation gets pancreatin finished product 3.40g behind the degreaser drying, weight recovery is 17.0%, trypsinase, lipase, diastatic content are respectively 4 in the product, 100U/g, 26,000U/g, 50,000U/g.Supernatant liquor is for reclaiming PEG.
Example 4:(1) gets freezing pig pancreas or fresh pig pancreas and rub into the back activation of pancreas slurry.(2) get activation pancreas slurry 200g (being equivalent to pig pancreas 80g), add water to 1000mL, transferring pH is 7.0, adds PEG 150g, fully stir, precipitate 60min under 8 ℃ of conditions, add a spot of short precipitation agent then, fully stir, continue precipitation 6h under 8 ℃ of conditions, the centrifugal 10min of 3000rpm, collecting precipitation gets pancreatin finished product 14.4g behind the degreaser drying, weight recovery is 18.0%, trypsinase, lipase, diastatic content are respectively 2 in the product, 687U/g, 20,500U/g, 46,000U/g.Supernatant liquor is for reclaiming PEG.
Example 5:(1) gets freezing pig pancreas or fresh pig pancreas and rub into the back activation of pancreas slurry.(2) get activation pancreas slurry 200g (being equivalent to pig pancreas 80g), add water to 1000mL, transferring pH is 7.8, adds PEG 250g, fully stir, precipitate 90min under 4 ℃ of conditions, add a spot of short precipitation agent then, fully stir, continue precipitation 5h under 4 ℃ of conditions, the centrifugal 10min of 3000rpm, collecting precipitation gets pancreatin finished product 11.4g behind the degreaser drying, weight recovery is 14.3%, trypsinase, lipase, diastatic content are respectively 3 in the product, 400U/g, 27,500U/g, 57,000U/g.Supernatant liquor is for reclaiming PEG.
The PEG that reclaims from supernatant liquor can continue on for preparing pancreatin.
Example 6:(1) gets freezing pig pancreas or fresh pig pancreas and rub into the back activation of pancreas slurry.(2) get activation pancreas slurry 200g (being equivalent to pig pancreas 80g), add water to 1000mL, transferring pH is 7.8, adds to reclaim PEG 250g (using continuously 3 times), fully stirs, precipitate 90min under 4 ℃ of conditions, add a spot of short precipitation agent then, fully stir, continue precipitation 5h under 4 ℃ of conditions, the centrifugal 10min of 3000rpm, collecting precipitation gets pancreatin finished product 10.4g behind the degreaser drying, weight recovery is 13.0%, trypsinase, lipase, diastatic content are respectively 3 in the product, 600U/g, 24,000U/g, 50,000U/g.
Trypsinase, lipase, diastatic vitality test: with reference to 2000 editions CP.
Claims (7)
1. the new preparation process of high vigor pancreatin; precipitate activating the pancreas slurry with enzyme precipitation protective material; it is characterized in that to freeze pig pancreas or fresh pig pancreas and rub into the back activation of pancreas slurry, transfer to certain pH, add PEG (polyoxyethylene glycol) and fully stir; after precipitating for some time under the certain temperature condition; add a spot of short precipitation agent and fully stir, continue precipitation for some time, centrifugal; collecting precipitation gets the pancreatin finished product behind the degreaser drying.Supernatant liquor is for reclaiming PEG.
2. high vigor pancreatin new preparation process as claimed in claim 1 is characterized in that the enzyme precipitation protective material that uses is PEG.
3. high vigor pancreatin new preparation process as claimed in claim 1, the consumption that it is characterized in that PEG is 10～50%.
4. high vigor pancreatin new preparation process as claimed in claim 1, the pH when it is characterized in that precipitating is 6.0～8.0.
5. high vigor pancreatin new preparation process as claimed in claim 1 is characterized in that the independent sedimentary time of PEG is 30～120min.
6. high vigor pancreatin new preparation process as claimed in claim 1 is characterized in that adding that to continue the sedimentary time behind the short precipitation agent be 3～7 h.
7. high vigor pancreatin new preparation process as claimed in claim 1, the temperature when it is characterized in that precipitating is 2～8 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200410081389 CN1632114A (en) | 2004-12-03 | 2004-12-03 | Process for producing high active pancreatin |
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| CN 200410081389 CN1632114A (en) | 2004-12-03 | 2004-12-03 | Process for producing high active pancreatin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103343114A (en) * | 2013-07-22 | 2013-10-09 | 四川大学 | Method for preparing high-activity pancreatin |
| CN103571817A (en) * | 2013-11-05 | 2014-02-12 | 马忠仁 | Preparation method of pancreatic enzyme powder |
| CN107502349A (en) * | 2017-10-10 | 2017-12-22 | 广西师范学院 | The preparation method of water-soluble yellow fluorescence carbon quantum dot |
-
2004
- 2004-12-03 CN CN 200410081389 patent/CN1632114A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103343114A (en) * | 2013-07-22 | 2013-10-09 | 四川大学 | Method for preparing high-activity pancreatin |
| CN103343114B (en) * | 2013-07-22 | 2015-10-28 | 四川大学 | A kind of method preparing high vigor pancreatin |
| CN103571817A (en) * | 2013-11-05 | 2014-02-12 | 马忠仁 | Preparation method of pancreatic enzyme powder |
| CN107502349A (en) * | 2017-10-10 | 2017-12-22 | 广西师范学院 | The preparation method of water-soluble yellow fluorescence carbon quantum dot |
| CN107502349B (en) * | 2017-10-10 | 2021-01-22 | 苏州诺维康生物科技有限公司 | Preparation method of water-soluble bluish violet light carbon quantum dots |
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Open date: 20050629 |