CN1629618A - Multifunctional photoexcited surface plasma resonant imager - Google Patents
Multifunctional photoexcited surface plasma resonant imager Download PDFInfo
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Abstract
This invention multifunction imager is made based on the surface plasma resonance phenomenon composed of p-polarized parallel light sources, a light incident angle modulation system, a sample transport and analysis system, a reflection light cut-away and modulation system, a detector, and a data register and process system. Said device utilizes the dispersion of P-polarized light and a prism to activate the resonance of sensing film surface plasma to test resonance signals. The resonance image signal or wave length scan resonance signal can be tested by conversion of light sources, the chip research result can be reached by micro-lattice process to sense film to realize spectrum conversion of image and resonance wavelength.
Description
Technical field
The present invention relates to the detection technique field, particularly a kind of light excitation surface plasma resonance instrument system.Specifically light activated surface plasma body resonant vibration signal transition is become intuitive image, also can convert the resonant wavelength detection system to.
Background technology
The resonance that surface plasma body resonant vibration (Surface Plasmon Resonance is called for short SPR) causes based on P polarized light excitating surface plasma.When the free electron thin layer at its interface such as silver or gold thin film are propagated and be incident upon to the P polarized light in high refracting medium such as glass, can form resonance at certain angle or wavelength place, produce reflection loss, promptly locate to record the intensity of reflected light minimum, form negative peak in a certain angle (resonance angle) or wavelength (resonant wavelength).Resonance angle or resonant wavelength are very responsive to medium (the being sample) change of refractive near metal film, can reflect the composition and the character of sample thus, realize the real-time and harmless detection to sample.In principle, utilize the SPR principle, can set up methods such as SPR spectrum analysis, hyperchannel spectrum analysis and imaging analysis.Over past ten years, international and domestic a lot of to single channel and hyperchannel SPR spectrum analysis (also claiming sensing) method research, listing along with BiocoreAB company commercial apparatus, its application aspect bio-molecular interaction, carry out rapidly, promoted the development of instruments design research thus conversely, domestic existing SPR spectrum analysis instrument patent about mensuration angle (CN1364233A) and wavelength (CN1203382A), but belong to the single channel category.The latest model instrument is that (Uppsala Sweden), can carry out the four-way test to BIAcore 3000 simultaneously at present.For improving mensuration capacity and the finding speed of unit interval, the U.S. has developed the interactional intersection microchannel SPR imaging system of mensuration DNA, RNA and has applied for relevant patent (PUB.APP.NO.20030017579), this patent utilization silicon rubber is made the intersection microchannel, but complex manufacturing process, practicality is relatively poor.It is system about the SPR two-dimensional imaging that the U.S. also has a up-to-date patent (PUB.APP.NO.20030048452), but this patent still belongs to single function instrument, promptly only relate to two-dimensional imaging, extensibility is little, can not realize the related or function conversion between SPR spectrogram and the imaging.
Summary of the invention
The objective of the invention is, a kind of multifunctional light excitating surface plasma resonance imager is provided, the range of application that can extended surface plasma resonant imaging instrument and the phase co-conversion of resonance signal.
For achieving the above object, technical solution of the present invention provides a kind of multifunctional light excitating surface plasma resonance imager, by P-polarization parallel radiant, the angle of light modulating system, sample delivery and analytic system, reflected light correction and modulating system, detecting device, data recordin and procesin system is formed, it is on a platform, P-polarization parallel radiant is set, penetrate at P-polarization parallel radiant on the light path of light, be provided with the angle of light modulating system in regular turn, sample delivery and analytic system, reflected light correction and modulating system and detecting device, detecting device is electrically connected with data recordin and procesin system.
Described multifunctional light excitating surface plasma resonance imager, its described light source comprises the continuous wavelength light source, laser instrument or light emitting diode.
Described multifunctional light excitating surface plasma resonance imager, its described P-polarization parallel radiant, comprise laser instrument, optical beam expander, polarizer, wherein polarizer can be crystal or many interfaces transmission-reflection configuration, desirable polarizer energy polarization light output; Polarizer can be placed on signal and receive in the structure.
Described multifunctional light excitating surface plasma resonance imager, its described P-polarization parallel radiant, be made up of white light source, slit, monochromator, expansion bundle parallel optical structure, P-polaroid, wherein monochromator and polarizer can be placed in the receiving system.
Described multifunctional light excitating surface plasma resonance imager when it is basic light source with light emitting diode, does not need monochromator.
Described multifunctional light excitating surface plasma resonance imager, its described monochromator is optical filter or grating.
Described multifunctional light excitating surface plasma resonance imager, the laser instrument of its described P-polarization parallel radiant is single wavelength or multiple-wavelength laser.
Described multifunctional light excitating surface plasma resonance imager, its described laser instrument is He-Ne laser instrument, dye laser or semiconductor laser.
Described multifunctional light excitating surface plasma resonance imager, its described angle of light modulating system comprises incident light arm, input arm, prism, sensing tongue piece, accurate lead screw, motor, angular transducer, guide rail; Wherein, prism is fixed between incident light arm and the input arm, and the incident light arm is connected by screw with input arm top, but following can freely opening or shrink, incident light arm bottom is connected on the guide rail; Input arm bottom links to each other with the sensing tongue piece, drive the sensing tongue piece by angular transducer control motor by accurate lead screw and on guide rail, move around, thereby make incident light arm and input arm freely open or shrink the angle that reaches accurate control prism incident light.
Described multifunctional light excitating surface plasma resonance imager, adjusting degree of accuracy≤10 of its described angle of light modulating system
-3Degree.
Described multifunctional light excitating surface plasma resonance imager, its described sample delivery and analytic system, form by prism, sensing membrane, micro updating sample cell, injection port, outlet, micro pump and associated pipe, sensing membrane is the high reflecting metal film, prism and sensing membrane are used and the liquid medium adhesion that is complementary of prismatic refraction rate, use washer sealing between sensing membrane and the sample cell, the input and output of sample are controlled by micro pump.
Described multifunctional light excitating surface plasma resonance imager on its described sensing membrane, forms addressing sample microarray by the control chemical modification, is used for the identification and the detection of each sample molecule species, and this method is particularly suitable for the detection of biomacromolecule and cell.
Described multifunctional light excitating surface plasma resonance imager, its correction of described reflected light and modulating system, be with reflected light via cylinder or spherical reflector and the distortion of lens combination correcting image, and image is correctly focused on the detecting device.
Described multifunctional light excitating surface plasma resonance imager, its described detecting device is CCD camera, CMOS sensor, charge-injection detector(CID), normal optical fulgurite or photomultiplier.
Described multifunctional light excitating surface plasma resonance imager, it connects grating before detecting device when making light source with continuous wavelength light, promptly convert the surface plasma body resonant vibration instrument of measuring resonant wavelength to.
Described multifunctional light excitating surface plasma resonance imager, its described data recordin and procesin system is provided with computing machine, and detection data machine as calculated receive, store, handle and show or export.
Described multifunctional light excitating surface plasma resonance imager, its described data recordin and procesin system, the view data of its reception, available business graphics software processed offline, demonstration and output, or availablely show in real time, handle, show and export from working out software; Provide choosing at random and the real-time optimization function of the overall situation, topography's collection from establishment software, can be output as one dimension, two dimension or 3-D view to the surface plasma body resonant vibration signal in real time; Regulate and control arbitrarily but image shows local, and monitor the dynamic changing process of different sample spot signals simultaneously; Software possesses the qualitative, quantitative function.
Described multifunctional light excitating surface plasma resonance imager, its described metal film is gold or silverskin, thickness is between 20-80nm.
The characteristics of instrument of the present invention are:
1, instrument multifunction:
A) can be chosen to the control of picture scheme and image according to the experiment needs, can show one dimension, two and three dimensions image in real time, image show can the overall situation or local select arbitrarily;
B) can carry out dynamic process analysis simultaneously to each sample spot, form the high flux analytical instrument new technology;
C) can directly expand to the chip analysis technology;
D) can be embodied as picture and resonance wavelength spectrum map analysis conversion.
2, apparatus structure is flexible, convertible and can expand:
A) light-source system can be expanded and flexibly, available laser, white light, light emitting diode wait and produce the P-polarization, and polarization or unpolarized luminescence body can use;
B) image processing software can business software, also can be earlier with the multi-functional real-time processing software of the special configuration of native system;
C) can be according to actual sample needs conversion sensing membrane, and the production program of sensing membrane is simple, can adopt commercial Linomat, also can adopt self-control single head sample applicator to realize dot matrixization or chip processing to sensing membrane comprising addressing to sample;
D) there is multiple detecting device to select altogether, as CCD camera, scanning diode, photoelectric tube, photomultiplier, charge-injection detector(CID) and CMOS etc.
3, the incident angle that adopts optics and mechanical compound system to regulate light accurately;
4, analysis cell is simple in structure, makes easily, does not have Micro Channel Architecture, is suitable for the quick replacing of sensing membrane.
5, analytic target is wide, can be used for biology, biological chemistry, clinical medicine and relevant chemistry and physical detection; Can carry out interactional mensuration such as carbohydrate, DNA, RNA, protein and cell easily; Can carry out qualitative and quantitative analysis.Therefore can be the hybridization of DNA and the research of order-checking, Proteomic analysis and association area provides a kind of high flux or quick, cold check and analysis method.
Description of drawings
Fig. 1 is the basic structure synoptic diagram of light excitation surface plasma resonance imager of the present invention;
Fig. 2 is the polarization parallel radiant structural representation based on laser of light excitation surface plasma resonance imager of the present invention;
Fig. 3 is the light beam of light source collimating structure synoptic diagram of light excitation surface plasma resonance imager of the present invention;
Fig. 4 is the angle of light modulating system expansion structure of light excitation surface plasma resonance imager of the present invention;
Fig. 5 is sample delivery and analytic system expanded structure synoptic diagram in the light excitation surface plasma resonance imager of the present invention;
Fig. 6 is the resonance image-forming figure of bovine serum albumin white point in imager of the present invention;
Fig. 7 is the three-dimensional plot of the resonance image-forming of bovine serum albumin white point in imager of the present invention;
Fig. 8 is the linear superposition figure of the resonance figure of bovine serum albumin white point in imager of the present invention;
Fig. 9 is bovine serum albumin(BSA) and the resonance image-forming figure of polylysine in imager of the present invention;
Figure 10 is the three-dimensional plot of bovine serum albumin(BSA) and the resonance image-forming of polylysine in imager of the present invention;
Figure 11 is resonant wavelength is measured in bovine serum albumin(BSA) and glucose interaction in instrument of the present invention surface plasma body resonant vibration figure;
Figure 12～Figure 17 is a SPR software flow pattern of the present invention.
Embodiment
The present invention is a multifunctional light excitating surface plasma resonance imager, mainly be made up of with six parts such as modulating system D, detecting device E, data recordin and procesin system F P-polarization parallel radiant A, angle of light modulating system B, sample delivery and analytic system C, reflected light correction, basic structure as shown in Figure 1.On a platform, P-polarization parallel radiant A is set, penetrate at P-polarization parallel radiant A on the light path of light, be provided with angle of light modulating system B, sample delivery and analytic system C, reflected light correction and modulating system D and detecting device E in regular turn, detecting device E is electrically connected with data recordin and procesin system F.
1.P-polarization parallel radiant A: basic structure sees that accompanying drawing 2 comprises that (be made up of lens combination, lens combination comprises lens 2a, lens 2b, lens 2c, lens 2d for laser instrument 1, optical beam expander 2.), polarizer 3, wherein polarizer 3 can be crystal or many interfaces transmission-reflection configuration, desirable polarizer 3 can polarization light output.Polarizer 3 can be placed on signal and receive in the structure.Laser instrument 1 is changed according to the required wavelength of experiment, adopts red light semiconductor laser usually.
Expansion structure one: based on the light source of white light, its structure is seen accompanying drawing 3, be made up of white light source 4, slit 5, monochromator 6 (as optical filter or grating etc.), expansion bundle parallel optical structure 7 (being made up of 7a, 7b lens combination), P-polaroid 8 etc., wherein monochromator and polarizer can be placed in the receiving system.
Expansion structure two: based on the light source of light emitting diode, do not need monochromator, other structure is identical with white light source.
Expansion structure three: integrated light source, respectively various light sources are designed to one-piece construction with expanding bundle parallel optical structure and polarizer and monochromator, form independently integrated light source.
2. angle of light modulating system B: amplify adjusting portion by optical reflection part and machinery and be grouped into.Wherein optical mirror places on the mechanic adjustment unit, and mechanic adjustment unit can realize the meticulous angular adjustment of prism incident angle of light, regulates degree of accuracy and reaches 10
-3Degree or littler.
Expansion structure: structure sees that accompanying drawing 4 comprises incident light arm 9, input arm 10, prism 11, sensing tongue piece 12, accurate lead screw 13, motor 14, angular transducer 15, guide rail 16.
Prism 11 is fixed between incident light arm 9 and the input arm 10, and incident light arm 9 is connected by screw with input arm 10 tops, but following can freely opening or shrink, incident light arm 9 bottoms are connected on the guide rail 16; Input arm 10 bottoms link to each other with sensing tongue piece 12, drive sensing tongue piece 12 by angular transducer control motor 14 by accurate lead screw 13 and on guide rail 16, move around, thereby make incident light arm 9 and input arm 10 freely open or shrink the angle that reaches accurate control prism 11 incident lights.
3. sample delivery and analytic system C: mainly become with prism and relevant stream set of systems by micro pump, the flow rate of sample is controlled by micro pump, and sensing membrane sticks to the prism bottom, and finishes sample analysis.Its basic structure is seen accompanying drawing 5, comprises sample storage bottle 17, O-ring seal 18, pump 19, sample cell 20, sensing prism 21, sample flow direction 22, waste liquid bottle 23.
Prism 21 in the analytic system is made by optical glass usually, also can adopt other high index of refraction light transmissive material to manufacture.Sensing membrane is chemical modification gold or the silverskin that is plated on the prism surface, and the THICKNESS CONTROL of metal film is between 20-80nm.Sample is introduced sample cell 20 by the micro pump 19 of scalable flow velocity, by O-ring seal 18 sensing membrane is contacted sealing-in with prism 21.
Expansion structure: sample delivery and purging system automatic program control; Sensing prism 21 is made up of separate prism and vane, and middle sticking and medium by refractive index match is bonding; Sample analysis pond 20 bodies can design and change as required.
4. reflected light correction and modulating system D: mainly form, be used to proofread and correct the anamorphose and the deviation that are produced after by prism because of light by post lens or spherical reflector and lens.Utilize the post lens can elongate or shorten the rectification of image.The adjustment of scioptics group need make the image after the rectification just focus on the CCD detecting device E sensing surface.
5. detecting device E: select detecting device E and relevant configuration according to the experiment needs, in imaging arrangement, can from CCD camera, CMOS, charge-injection detector(CID) E, normal optical fulgurite or photomultiplier, select for use a kind of, detecting device E can receive the whole light beams that reflect from sensing membrane, shows resonance image-forming figure in real time.
Expansion structure one: detecting device E can select dot matrix or line array CCD and scanning photomultiplier, the imaging by quick scanning for use.
Expansion structure two: convert the surface plasma body resonant vibration instrument of measuring resonant wavelength as need to, use the continuous wavelength light source, and select for use and imaging arrangement similar detection device E, but should connect grating before the detecting device E, the light that reception reflects from sensing membrane (also available fiber receives and it is transferred to the grating) is by detecting device E disclosing solution long scan resonance signal.
6. control and the F of data recording and processing system: this part mainly is made up of computing machine and software, finishes tasks such as system's control and signal record, processing and output.It both can be business graphics software that image recording is handled used software, also available self-programmed software.Utilize business software generally can only gather two dimensional image, related data needs off-line analysis; Utilize software provided by the invention can the realization system control, demonstrations in real time, off-line demonstrations, data subsequent treatment, figure and operations such as selection of resonance line signal and conversion.Self-programmed software designs according to the experiment needs, the signal that can carry out same image in the two dimensional image and different images relatively, any regulation and control of realization image also can be monitored the dynamic process of a plurality of sample spot simultaneously, spr signal can be output as the conversion of one dimension, two dimension and three-dimensional collection of illustrative plates and the spectrogram of being correlated with in real time, realizes qualitative and quantitative analysis.
Self-programmed software comprises: data acquisition, performance analysis, static analysis, control system and data access.Part of data acquisition is to select suitable collection form and acquisition window number according to the experiment needs, and can carry out the conversion of picture format, realizes and other business software compatibility.Performance analysis is in order to obtain the Dynamic Signal of SPR, and this part can strengthen signal to noise ratio (S/N ratio), can carry out the spectrum analysis of monocyte sample point and many sample spot, observes the interactional dynamic process of different sample spot in real time.Static analysis mainly is to finish quantitative test and 3-D view processing, obtains the abundant more interactional qualitative, quantitative information of sample spot.Various piece is in optimized state to the control system part in the instrument in order to make, and can realize the regulation and control of flow, speed and the acquisition controlling of light path system, sample analysis system, can accurately regulate the incident angle of light, and instrument is worked under top condition.Data access comprises data read, format conversion and data preservation, realizes the compatibility with other relative commercial software.
The total system principle of work: P polarization parallel light A enters angle of light modulating system B, the B of this system enters sample delivery and analytic system C with optical modulation after required angle, excite prism bottom sensing membrane surface plasma and produce resonance, directly cause the variation of intensity of reflected light.The reflected light that penetrates from prism enters reflected light correction and modulating system D, and reflected light correction and the distortion of modulating system D correcting image correctly focus on the detecting device E image after the rectification.Detecting device E voltage signal is by high-speed a/d interface such as image pick-up card, convert digital signal to, send into data recordin and procesin system F computing machine, computing machine reaches the qualitative and quantitative analysis purpose with the gained data by software processes, and realizes the real-time screen display of image.
Embodiment one:
The prism bottom is coated with the sensing membrane of the thick golden film of one deck 50nm as the height reflection, and sensing membrane is at first handled with the sulfydryl undecanoic acid, goes up the bovine serum albumin white point by the chemical modification assembling.Instrument adopts CCD to make detecting device, and the surface plasma resonance image-forming figure in solution sees Fig. 6, Fig. 7, and Fig. 8 is respectively three-dimensional plot and linear superposition figure.
Embodiment two:
The method identical with embodiment one usefulness modified sensing membrane, modifies bovine serum albumin(BSA) and polylysine respectively.Instrument adopts CCD to make detecting device, and the surface plasma resonance image-forming figure in solution sees Fig. 9 (bovine serum albumin(BSA) is classified on a left side three as, and right three classify polylysine as), and Figure 10 is its three-dimensional plot.
Embodiment three:
Be fixed into shooting angle and adopt CCD to make detecting device and connect grating, modify sensing membrane, be connected to bovine serum albumin(BSA) on the sensing membrane with embodiment one usefulness similar methods.At first feed buffer solution toward sample cell, and then feed the glucose sample that is assigned in the identical buffer solution, start real-time detecting operation simultaneously, respectively the variation of record buffer solution and glucose solution resonant wavelength, see Figure 11, realize real-time and performance analysis thus glucose.
SPR software flow of the present invention is asked for an interview Figure 12～Figure 17.
Claims (18)
1. multifunctional light excitating surface plasma resonance imager, form by P-polarization parallel radiant, angle of light modulating system, sample delivery and analytic system, reflected light correction and modulating system, detecting device, data recordin and procesin system, it is characterized in that, on a platform, P-polarization parallel radiant is set, penetrate at P-polarization parallel radiant on the light path of light, be provided with angle of light modulating system, sample delivery and analytic system, reflected light correction and modulating system and detecting device in regular turn, detecting device is electrically connected with data recordin and procesin system.
2. multifunctional light excitating surface plasma resonance imager as claimed in claim 1 is characterized in that described light source comprises the continuous wavelength light source, laser instrument or light emitting diode.
3. multifunctional light excitating surface plasma resonance imager as claimed in claim 1, it is characterized in that, described P-polarization parallel radiant, comprise laser instrument, optical beam expander, polarizer, wherein polarizer can be crystal or many interfaces transmission-reflection configuration, the energy polarization light output; Polarizer can be placed on before signal receives detecting device in the structure.
4. multifunctional light excitating surface plasma resonance imager as claimed in claim 1, it is characterized in that, described P-polarization parallel radiant, form by white light source, slit, monochromator, expansion bundle parallel optical structure, P-polaroid, before wherein monochromator and polarizer can be placed on detecting device in the receiving system.
5. multifunctional light excitating surface plasma resonance imager as claimed in claim 4 is characterized in that, when being basic light source with light emitting diode, does not need monochromator.
6. as claim 4 or 5 described multifunctional light excitating surface plasma resonance imagers, it is characterized in that described monochromator is optical filter or grating.
7. multifunctional light excitating surface plasma resonance imager as claimed in claim 1 is characterized in that, the laser instrument of described P-polarization parallel radiant is single wavelength or multiple-wavelength laser.
8. as claim 2,3 or 7 described multifunctional light excitating surface plasma resonance imagers, it is characterized in that described laser instrument is He-Ne laser instrument, dye laser or semiconductor laser.
9. multifunctional light excitating surface plasma resonance imager as claimed in claim 1, it is characterized in that, described angle of light modulating system comprises incident light arm, input arm, prism, sensing tongue piece, accurate lead screw, motor, angular transducer, guide rail; Wherein, prism is fixed between incident light arm and the input arm, and the incident light arm is connected by screw with input arm top, but following can freely opening or shrink, incident light arm bottom is connected on the guide rail; Input arm bottom links to each other with the sensing tongue piece, drive the sensing tongue piece by angular transducer control motor by accurate lead screw and on guide rail, move around, thereby make incident light arm and input arm freely open or shrink the angle that reaches accurate control prism incident light.
10. as claim 1 or 9 described multifunctional light excitating surface plasma resonance imagers, it is characterized in that adjusting degree of accuracy≤10 of described angle of light modulating system
-3Degree.
11. multifunctional light excitating surface plasma resonance imager as claimed in claim 1, it is characterized in that, described sample delivery and analytic system, form by prism, sensing membrane, micro updating sample cell, injection port, outlet, micro pump and associated pipe, sensing membrane is the high reflecting metal film, prism and sensing membrane are used and the liquid medium adhesion that is complementary of prismatic refraction rate, use washer sealing between sensing membrane and the sample cell, and the input and output of sample are controlled by micro pump.
12. multifunctional light excitating surface plasma resonance imager as claimed in claim 11, it is characterized in that, on the described sensing membrane, form addressing sample microarray by the control chemical modification, be used for the identification and the detection of each sample molecule species, this method is particularly suitable for the detection of biomacromolecule and cell.
13. multifunctional light excitating surface plasma resonance imager as claimed in claim 1, it is characterized in that, correction of described reflected light and modulating system, be with reflected light via cylinder or spherical reflector and the distortion of lens combination correcting image, and image is correctly focused on the detecting device.
14. multifunctional light excitating surface plasma resonance imager as claimed in claim 1 is characterized in that, described detecting device is CCD camera, CMOS sensor, charge-injection detector(CID), normal optical fulgurite or photomultiplier.
15. multifunctional light excitating surface plasma resonance imager as claimed in claim 2 is characterized in that, when making light source with continuous wavelength light, connects grating before the detecting device, promptly converts the surface plasma body resonant vibration instrument of measuring resonant wavelength to.
16. multifunctional light excitating surface plasma resonance imager as claimed in claim 1 is characterized in that described data recordin and procesin system is provided with computing machine, detection data machine as calculated receive, store, handle and show or export.
17. as claim 1 or 16 described multifunctional light excitating surface plasma resonance imagers, it is characterized in that, described data recordin and procesin system, the view data of its reception, available business graphics software processed offline, demonstration and output, or with showing in real time, handle, show and export from working out software; Provide choosing at random and the real-time optimization function of the overall situation, topography's collection from establishment software, can be output as one dimension, two dimension or 3-D view to the surface plasma body resonant vibration signal in real time; Regulate and control arbitrarily but image shows local, and monitor the dynamic changing process of different sample spot signals simultaneously; Software possesses the qualitative, quantitative function.
18. as claim 11 or 12 described multifunctional light excitating surface plasma resonance imagers, it is characterized in that described metal film is gold or silverskin, thickness is between 20-80nm.
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| CN100545631C (en) * | 2006-05-18 | 2009-09-30 | 中国科学院化学研究所 | Multifunctional light absorption, scattering and emission spectrometer based on surface plasma-wave |
| CN100575927C (en) * | 2006-05-18 | 2009-12-30 | 中国科学院化学研究所 | Resonance imager of light-exciting color surface piasma |
| CN102419316A (en) * | 2010-09-28 | 2012-04-18 | 北京金菩嘉医疗科技有限公司 | Microbeam multi-channel parallel surface plasmon resonance detection system |
| CN102460120A (en) * | 2009-04-07 | 2012-05-16 | 莱尔照明公司 | Peri-critical reflection spectroscopy devices, systems, and methods |
| CN102519914A (en) * | 2011-12-22 | 2012-06-27 | 中国科学院理化技术研究所 | Wavelength modulation surface plasma resonance detection device based on laser confocal imaging |
| CN103080726A (en) * | 2010-09-13 | 2013-05-01 | 松下电器产业株式会社 | Method for measuring concentration of antigen contained in test solution |
| CN103472038A (en) * | 2013-09-16 | 2013-12-25 | 浙江大学 | Optimized area-array type SPR (Surface Plasmon Resonance) sensor |
| CN103674902A (en) * | 2013-12-12 | 2014-03-26 | 中国科学院半导体研究所 | LSPR (Localized Surface Plasmon Resonance) portable biochemical detector based on mobile phone platform |
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| CN103698304A (en) * | 2014-01-15 | 2014-04-02 | 中国科学院化学研究所 | Shear type liquid core coupled surface plasma resonance imaging analyzer |
| US8730468B2 (en) | 2008-02-01 | 2014-05-20 | Rare Light, Inc. | Methods, devices and kits for peri-critical reflectance spectroscopy |
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| CN107219199A (en) * | 2017-07-24 | 2017-09-29 | 深圳大学 | Novel angle modulation SPR imaging systems based on 4F systems |
| CN107764776A (en) * | 2017-04-18 | 2018-03-06 | 南京大学 | Multi-wavelength adjustable meter surface plasma resonance imaging device and its application |
| CN108700775A (en) * | 2016-01-27 | 2018-10-23 | 夏普株式会社 | Wavelength conversion substrate, liquid crystal cell, Liquid Crystal Module and liquid crystal display device |
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| CN100575927C (en) * | 2006-05-18 | 2009-12-30 | 中国科学院化学研究所 | Resonance imager of light-exciting color surface piasma |
| CN100545631C (en) * | 2006-05-18 | 2009-09-30 | 中国科学院化学研究所 | Multifunctional light absorption, scattering and emission spectrometer based on surface plasma-wave |
| US8730468B2 (en) | 2008-02-01 | 2014-05-20 | Rare Light, Inc. | Methods, devices and kits for peri-critical reflectance spectroscopy |
| CN102460120A (en) * | 2009-04-07 | 2012-05-16 | 莱尔照明公司 | Peri-critical reflection spectroscopy devices, systems, and methods |
| US9041923B2 (en) | 2009-04-07 | 2015-05-26 | Rare Light, Inc. | Peri-critical reflection spectroscopy devices, systems, and methods |
| CN102460120B (en) * | 2009-04-07 | 2014-07-30 | 莱尔照明公司 | Peri-critical reflection spectroscopy devices, systems, and methods |
| CN103080726A (en) * | 2010-09-13 | 2013-05-01 | 松下电器产业株式会社 | Method for measuring concentration of antigen contained in test solution |
| CN103080726B (en) * | 2010-09-13 | 2015-07-29 | 松下电器产业株式会社 | For measuring the method for the concentration of the antigen be contained in test solution |
| CN102419316A (en) * | 2010-09-28 | 2012-04-18 | 北京金菩嘉医疗科技有限公司 | Microbeam multi-channel parallel surface plasmon resonance detection system |
| US8970838B2 (en) | 2011-04-29 | 2015-03-03 | Avolonte Health LLC | Method and apparatus for evaluating a sample through variable angle Raman spectroscopy |
| CN102519914B (en) * | 2011-12-22 | 2014-11-26 | 中国科学院理化技术研究所 | Wavelength modulation surface plasma resonance detection device based on laser confocal imaging |
| CN102519914A (en) * | 2011-12-22 | 2012-06-27 | 中国科学院理化技术研究所 | Wavelength modulation surface plasma resonance detection device based on laser confocal imaging |
| CN103472038A (en) * | 2013-09-16 | 2013-12-25 | 浙江大学 | Optimized area-array type SPR (Surface Plasmon Resonance) sensor |
| CN103674902A (en) * | 2013-12-12 | 2014-03-26 | 中国科学院半导体研究所 | LSPR (Localized Surface Plasmon Resonance) portable biochemical detector based on mobile phone platform |
| CN103698304A (en) * | 2014-01-15 | 2014-04-02 | 中国科学院化学研究所 | Shear type liquid core coupled surface plasma resonance imaging analyzer |
| CN103698306A (en) * | 2014-01-15 | 2014-04-02 | 中国科学院化学研究所 | Liquid core coupling surface plasma resonance imaging analyzer |
| CN103698304B (en) * | 2014-01-15 | 2016-04-13 | 中国科学院化学研究所 | A kind of scissors liquid core coupled surface plasmons resonance imaging analyzer |
| CN103698306B (en) * | 2014-01-15 | 2017-02-01 | 中国科学院化学研究所 | Liquid core coupling surface plasma resonance imaging analyzer |
| CN104849237A (en) * | 2015-05-25 | 2015-08-19 | 黑龙江大学 | Refractive index measuring device based on wavelength modulation SPR (surface plasmon resonance) |
| CN108700775A (en) * | 2016-01-27 | 2018-10-23 | 夏普株式会社 | Wavelength conversion substrate, liquid crystal cell, Liquid Crystal Module and liquid crystal display device |
| CN107764776A (en) * | 2017-04-18 | 2018-03-06 | 南京大学 | Multi-wavelength adjustable meter surface plasma resonance imaging device and its application |
| CN107764776B (en) * | 2017-04-18 | 2020-05-05 | 南京大学 | Multi-wavelength adjustable surface plasma resonance imaging device and application thereof |
| CN107219199A (en) * | 2017-07-24 | 2017-09-29 | 深圳大学 | Novel angle modulation SPR imaging systems based on 4F systems |
| CN110887815A (en) * | 2019-12-26 | 2020-03-17 | 朱先伟 | Optical detection system adopting multi-lens switching structure |
| CN112858226A (en) * | 2021-03-01 | 2021-05-28 | 江苏科技大学 | Dynamic visible spectrometer surface plasma resonance measuring device |
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