CN1615436A - Water monitoring method using algae - Google Patents
Water monitoring method using algae Download PDFInfo
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- CN1615436A CN1615436A CNA028273125A CN02827312A CN1615436A CN 1615436 A CN1615436 A CN 1615436A CN A028273125 A CNA028273125 A CN A028273125A CN 02827312 A CN02827312 A CN 02827312A CN 1615436 A CN1615436 A CN 1615436A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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Abstract
The present invention relates to a water monitoring method using algae, wherein the degree of pollution in a river can be monitored by using the algae, particularly, closterium ehrenbergii. The water monitoring method using the algae according to the present invention comprises the steps of taking raw water from a water system, adding a culture solution in which the closterium ehrenbergii has been cultivated to the taken raw water, measuring the respective amounts of fluorescence in the raw water with the culture solution added thereto and in the culture solution and comparing the amounts of fluorescence with each other, and analyzing the compared amounts of fluorescence and outputting the presence of water pollution as quantified data. Therefore, according to the present invention, there is an advantage in that the presence of the water pollution can be measured and monitored in real time. In addition, since the quantitative analysis of the degree of water pollution can be made, there is another advantage in that the degree of water pollution can be precisely expressed as numerical values. Moreover, there is a further advantage in that the number of relevant equipment and manpower required for the water monitoring can be remarkably reduced.
Description
Technical field
The present invention relates to a kind of method of utilizing algae to carry out aquatic monitoring, more particularly, relate to a kind of by observing the photosynthesis that can carry out photosynthetic algae such as green alga come the particularly method of water body environment of monitoring of environmental.
Background technology
Usually, utilize biologies such as fish, shellfish the method as area monitoring nuisance incidents of leakage such as rivers to be called as the method for biological monitoring.Since in the later stage seventies, whether Germany utilizes the method for fish or water flea etc. to be determined to have in the water system that comprises lake, river noxious material to invade, and comprises the biological monitoring system that begins one's study of the many countries of New Zealand, Belgium, France, Switzerland, Austria, Britain or the like.So far, be suitable for measuring the different aquatic organisms whether water system has noxious material to invade, carried out various research at those.Also therefore proposing many abnormal behaviour pattern analysis of aquatic organism or biological natures utilized monitors the method whether water body pollutes.
As in the biological monitoring that utilizes fish to carry out, people are to the behavior pattern of fish, as swim, Ambient, bunch, scatter and move about and carry out observation analysis.If the abnormal behaviour pattern appears in the fish of observing, just indicate the pollution of water system, as being arranged in the water system, noxious material invades.
Yet, influence the environmental factor that not only comprises noxious material of fish behavior pattern, and also have internal factors such as other healthy fish situations or other instinct requirements.Therefore, caused such problem:, the error of measuring usually occurs because the causal relation between behavior pattern of fish and the water pollution is not tight.
Furtherly, only when pollution level exceeded predetermined extent, water polluted the behavior pattern that just can influence fish.Owing to do not reaching under the situation of predetermined extent, the behavior pattern of fish will can not change, and whether this just can not mensuration exist water to pollute.And, even polluting, water goes beyond the limit of degree, and fish can not show the abnormal behaviour pattern immediately yet, and kind and degree according to polluting have certain preclinical delay, just can show unusual.That is to say that fish are less relatively to the susceptibility that water pollutes.
A major subjects of research biological monitoring improves this causal relation and susceptibility exactly.
Although water flea shows stronger this causal relation and susceptibility, but still abundant inadequately.
Effective method is to utilize luminescent microorganism recently.
Korea S 10-0262681 number, of E.I.Du Pont Company (E.I.Du Pont De Nemours and Company) is called the patent of " monitoring of environmental is polluted high responsive method "; Korea S 10-0300445 number, of Ou Shengken (Seong-Keun Oh) is called the patent of " utilizing luminescent microorganism to continue to monitor the Method and kit for of noxious material in the water system "; Ou Shengken (Seong-KeunOh) Korea S 10-0305218 name is called the patent of " utilizing fixing luminescent microorganism to measure the equipment of water toxicity automatically "; The LG company of Korea S's publication publication (Korean Laid-Open PatentPublication) number of the edition 2000-0024847 record (LG Industrial SystemsCo., Ltd.), name is called the document of " utilizing the fixedly method of luminescent microorganism and bio-sensing instrument mensuration noxious material "; Hundred O 'Neills (Bioneer) company of Korea S publication publication number of the edition 2001-0086342 record, name is called the document of " equipment that continues test water toxicity ", and other patent documents all disclose the technology of utilizing the luminescent microorganism monitoring of environmental to pollute.
Utilize in the biological monitoring of luminescent microorganism, measure the microbial luminescence degree change and come the prediction loop environment pollution.Yet the microbial luminescence degree change not only depends on environmental factor, and owing to various internal factors also change.That is to say, utilize the also not causal relation closely of biological monitoring environmental pollution of luminescent microorganism.
Summary of the invention
The purpose of this invention is to provide a kind of environmental pressure is had be closely connected and high sensitive method of environmental monitoring.
Purpose of the present invention also will provide the method for utilizing algae to carry out water monitoring, algae is sensitive to the intrusion of the noxious material in the water system, can and be expressed as digital quantity with the degree quantification of noxious material, can also realize monitoring in real time the situation that water pollutes, realize the quantitative test of water levels of contamination, and can obviously reduce material resources, manpower requirement that water detects.
Influence photosynthetic factor light intensity, gas concentration lwevel and temperature are arranged.That is to say that the photosynthetic total amount of healthy photosynthetic vegetable cell only is subjected to the influence of said external factor, is affected by other factors hardly under the situation of getting rid of the cell health condition.
According to the difference of influence factor, the variation of concrete vegetable cell photosynthesis quantity can be obtained by experiment in advance, and if the quantity of cell enough, then the probability that exists of healthy cell descends to some extent according to the difference of environmental baseline separately under normal distribution.
That is to say, can estimate that under varying environment condition separately, if award compensation according to the variation of influence factor, the photosynthetic total amount of capacity vegetable cell is similar to a steady state value.
Simultaneously, the healthiness condition of metaphyte needs the plenty of time with the variation of environmental factor, and on the contrary, healthiness condition and the metabolism of one-celled plants almost change immediately with environmental factor.
When considering these factors, can expect, utilize the photosynthetic total amount of enough one-celled plants to realize being closely connected and the environmental monitoring system of hypersensitivity at environmental pressure.
Another object of the present invention provides a kind of method of environmental monitoring of monitoring of environmental pressure of the photosynthesis total amount by observation photosynthesis algae.
In addition, consider each luminous energy that can realize photosynthetic surperficial incident stream, the part of luminous energy at first by each surperficial reflex as catoptrical energy to external radiation, the luminous energy that only retains is absorbed for each individuality.Be the energy of individual bulk absorption, major part is used for photosynthesis; The energy part that is not used for photosynthetic absorption then is converted into heat energy, and to each individual external release successively, energy remaining is converted into light wave to each external body radiation successively.
For body absorb to the light wave of external radiation and incident light or reflected light without any getting in touch, have single-frequency according to the difference of species.This light wave is also referred to as fluorescence.
In luminescent microorganism routine luminous, light wave is in the certain frequency scope, and this scope comprises visible light; This is luminous can not to have spontaneous generation under the situation of extraneous incident light, and to extraneous radiation.Opposite, when incident light disappeared, fluorescence mentioned above disappeared immediately or after the lasting short period, and had a wavelength that exceeds visible-range usually.
Experiment finds that intensity of fluorescence is inversely proportional to photosynthetic total amount.That is to say that when individuality was finished photosynthetic the work, fluorescence intensity died down; Otherwise, when because metabolism and healthiness condition unusual, when the photosynthetic activity of individuality weakens, the fluorescent brightness grow.
Therefore, the method for environmental monitoring according to the present invention utilizes photosynthetic algae, by measuring the fluorescence total amount, observing photosynthetic total amount, and can monitoring of environmental pressure.
Method of environmental monitoring of the present invention can be used for water system and monitor water pollution.
The method of this monitoring water pollution may further comprise the steps: the step of extracting the water body sample from the water system of needs monitoring water pollution; Preparation includes the step of the liquid reactants of photosynthesis algae; Preparation comprises the step of the mixing material of liquid reactants and water body sample; The step that liquid reactants and mixing material are carried out illumination; Measure the step of the fluorescent brightness that liquid reactants and mixing material send respectively; The step that the fluorescent brightness that will measure from liquid reactants and measure from mixing material compares; And the step of estimating the degree of water pollution according to the comparative result that obtains in the comparison step.
Another aspect of the present invention by the valuation to this monitored water pollution, can be realized the method for monitoring water pollution in the water system.This method is further comprising the steps of: the reference value of preliminary election water pollution warning; Compare the estimated value and the reference value that obtain based on the valuation step; And the result that reference value and estimated value obtained based on the comparison carries out early warning.
The more effective mode of this method can also may further comprise the steps: the measured value of measuring process gained is converted to digital value; And the step of in database, storing digital value.At this moment, in estimating step, can estimate the degree that water pollutes, from relevant water system sampled point numeral that obtain, that be stored in database value, estimate the degree that water pollutes in writing down by reference measure.
Furtherly, this method can also comprise the step of in the illumination step behavior pattern that has the photosynthesis algae in liquid reactants and the mixing material being observed respectively.At this moment, the valuation operation can realize based on the behavior pattern of observing photosynthesis algae in the step in the valuation step.
The mode that is more suitable for, the present invention also is included in the image-forming step of respectively behavior pattern that has the photosynthesis algae in liquid reactants and the mixing material being distinguished imaging in the illumination step; And the image that obtains in the image-forming step outputed on the display device.At this moment, in observing step, can have the behavior pattern of photosynthesis algae by the image viewing that outputs to imaging device.
Description of drawings
In conjunction with the accompanying drawings, by reading the hereinafter explanation of preferred embodiment, can more be expressly understood the present invention's characteristics mentioned above and other characteristics, advantage and other aspects.Description of drawings is as follows:
Fig. 1 utilizes algae to realize the description of flow diagram of the preferred embodiment of aquatic monitoring method according to the present invention
Fig. 2 is for carrying out in the method shown in Figure 1, when finishing photosynthetic illumination, in order to measure fluorescent brightness, and drips to synoptic diagram on the palette with liquid reactants with by the mixing material point that liquid reactants and sample liquid constitute.
Embodiment
The preferred embodiment of the present invention is to utilize a kind of Closterium Alan algae (closterium ehrenbergii) that belongs to green alga, monitors water pollution in water system, and Fig. 1 has provided the schematic flow sheet of the aquatic monitoring method of describing according to present embodiment.
As shown in Figure 1, the method according to the present embodiment aquatic monitoring comprises: the step S80 and the early warning step S90 that prepare the step S60 of step S00, the sampling step S10 of reference value, the step S20 for preparing liquid reactants, blend step S30, illumination step S40, the step S50 that measures amount of fluorescence, comparison fluorescence total amount, the step S70 that estimates the water pollution degree, contrast estimated value.
In the step S00 for preparing reference value, predetermined a plurality of reference values.These reference values are represented the degree of water pollution; According to the operating position of this value and water system and the kind of pollutant, to keeper's early warning.
In sampling step S10, need from the water system of water pollution to be monitored, extract the water body sample that uses, then it is stored in the cistern.
In the step S20 for preparing liquid reactants, ready Closterium Alan algae culturing liquid is used to monitor water pollution as liquid reactants.Closterium Alan algae quantity was preferably in nutrient solution and kept constant in the debita spissitudo scope this moment.And if a kind of method that is used to compensate according to the measured value of the variation of sum is provided, the nutrient solution of metering Closterium Alan algae quantity can replace constant number to use.The nutrient solution of preparing, as shown in Figure 2, point drips on two positions of palette 10.In the present embodiment, at the nutrient solution of first place value, 11 about drop 1ml of palette 10, at the nutrient solution of the second place 12 about drop 2ml of palette 10 as liquid reactants.
In blend step S30, extract about 1ml water body and drip on the palette 10 in the primary importance 11 of drop liquid reactants, and mix with it as the sample liquids point.
In illumination step S40, be used to realize the photosynthesis of Closterium Alan algae with liquid reactants on the rayed palette 10 and mixing material.
In the step S50 that measures amount of fluorescence, the amount of fluorescence that amount of fluorescence that the mixing material of the primary importance 11 of measurement palette 10 sends and the liquid reactants of measuring the second place 12 of palette 10 send.At this moment, the photofluorometer that can buy on market of utilization is measured amount of fluorescence.
In the step S60 that compares amount of fluorescence, will compare from liquid reactants amount of fluorescence that records and the amount of fluorescence that from mixing material, records.
In the step S70 that estimates the water pollution degree, estimate the water pollution degree according to the comparative result that in the S60 step, obtains.
Simultaneously, not having Closterium Alan algae in the assumes samples water body, approximately is in the second place 12 liquid reactants half of Closterium Alan algae sum according to certain mixture ratio by mixing the contained Closterium Alan algae sum in 11 the mixing material of being in the first position that obtains according to present embodiment then.Therefore, the amount of fluorescence of measuring in step S50 also will be only about half of degree.If there is Closterium Alan algae in the sample water body, then Zong Shuo ratio and the ratio that therefore obtains amount of fluorescence will increase.And if the result is tested and write down to the sample that extracts from same water system in advance, test result will be reflected on the estimated value of step S70, and the difference of this sum is sure.
In the step S80 of contrast estimated value, the reference value in step S70 gained estimated value and the S00 step compares.
In early warning step S90, carry out early warning to the water system keeper according to the result who contrasts the acquisition of reference value and estimated value.
In the example that present embodiment is described, the nutrient solution that contains Closterium Alan algae is as liquid reactants.Yet if exist a kind of algae culture liquid can monitor out the more polluter of high probability as more suitable liquid reactants in water system, this cultivation also can be used as liquid reactants and uses.If desired, the mixture of two or more algae culture liquid also can be used as liquid reactants.
In addition, according to present embodiment, this method can also comprise the step S51 of digitized measured value, step S52, the step S41 of imaging behavior pattern, the step S42 of display image and the step S43 of observed behavior pattern of store measurement values.
In the step S51 of digitized measured value, the measured value that will obtain in measuring process changes into digital value.The digital value that has transformed stores in the database in the step S52 of store measurement values.In this manner, estimate that the step S70 of water pollution degree can be in considering historical record from relevant water system sampling, be stored under the situation of the sample of digital value the database, estimate the water pollution degree.
In illumination step S40, finish the step S41 of imaging behavior pattern, to the behavior pattern imaging respectively that is present in algae photosynthesis in liquid reactants and the mixing material.In being shown as the step S42 of picture, the image of S41 gained outputs to display device.In the step S43 of observed behavior pattern, the behavior pattern of the image viewing photosynthesis algae by outputing to display device.Under this mode, observe the behavior pattern of photosynthesis algae according to the observation in the step, finish estimating of estimated value in the estimating step.
In the step S43 of observed behavior pattern, by the behavior pattern of the photosynthesis algae in microscope, endoscope or other device difference Direct observation liquid reactants and the mixing material.Because the Closterium Alan algae in the present embodiment has the cell bigger than other algae, therefore individual behavior pattern also facilitates observation.
The measured value that in step S50, obtains can be quantitative be expressed as the digital value of storage successively, therefore more suitable in the analytical work of research correlation water.
According to the aquatic monitoring system that present embodiment is realized, consider that its operation steps can partly or entirely be finished by computer control automatically.In this case, though can utilize numerical analysis, digital management and other operations of program realization measured value, the details description also can be left in the basket.
The method according to this invention is carried out in an output step and the described data interlock to of the image display step output unit.Therefore, the user promptly can obtain the quantitative data of water pollution degree by the output step, and can export step by imaging again and obtain image information, even at for example breadboard given position.Therefore the pollution level of water body in can the monitoring water system of multi-angle.Furtherly, according to the inventive method, owing to do not need physical chemistry method consuming time to measure the pollution of water body in the water system, its advantage is, can measure and monitor water pollution in real time.In addition, finish the quantitative test of water pollution owing to rely on environmental stimuli by the measurement of algae fluorescence, its another advantage is that water pollution degree can accurately be expressed as digital form.And, because water quality measurement only needs step simply and easily, from water system, to extract the sample water body and measure the algae amount of fluorescence by photofluorometer, its further advantage is, no longer need a large amount of devices and apparatus to be used for water quality measurement, therefore save the required man power and material of a large amount of aquatic monitorings.
Though the present invention has provided description in conjunction with the preferred embodiments, describe and be not used in qualification the present invention, but schematically explanation.Obviously, those skilled in the art can realize the method for various modifications, variation or adjustment under the situation that does not break away from the technology of the present invention essence and scope.The scope of the claim that therefore, depends on also comprises the method for various modifications, variation or adjustment.
Claims (7)
1, a kind of method of monitoring water pollution may further comprise the steps:
From the water system of water pollution to be monitored, extract the sample water body;
Preparation comprises the liquid reactants of photosynthesis algae;
Prepare the mixing material of liquid reactants and sample water body;
Illumination liquid reactants and mixing material;
The quantity of the fluorescence of measuring liquid reactants and mixing material respectively and being sent;
The liquid reactants of having measured more and the quantity of fluorescence that mixing material sends;
Estimate the degree of water pollution according to the comparing result that obtains in the comparison step.
2, according to the described method of claim 1, further comprising the steps of:
The reference value of water pollution alarm is sent in preparation;
The estimated value and the reference value that obtain in the estimating step are compared;
Result according to comparison reference and estimated value gives the alarm.
3, according to the described method of claim 1, further comprising the steps of:
The measured value that measuring process is obtained is converted into digital value,
In database, store digital value;
Wherein in estimating step, estimation water pollution degree consideration sample is measured historical record and is made, and this historical record derives from the digital value that is stored in relevant water system in the database.
4, according to the described method of claim 1, further comprising the steps of:
In illumination step, observe the behavior pattern of photosynthesis algae in liquid reactants and the mixing material respectively;
Wherein the behavior pattern of photosynthesis algae in the step is according to the observation made estimation in estimating step.
5, according to the described method of claim 4, further comprising the steps of:
In illumination step respectively to the behavior pattern imaging of the photosynthesis algae of liquid reactants and mixing material;
The image that will obtain in image-forming step outputs in the display device;
Wherein in observing step, the behavior pattern of the image viewing photosynthesis algae by outputing to display device.
According to arbitrary described method among the claim 1-5, it is characterized in that 6, according to the kind of pollutant, photosynthetic algae comprises two kinds of algae with different causal relations and susceptibility at least
According to arbitrary described method among the claim 1-5, it is characterized in that 7, photosynthetic algae comprises Closterium Alan algae.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020010079492A KR20020023747A (en) | 2001-12-14 | 2001-12-14 | Water monitoring method using algae |
| KR2001/0079492 | 2001-12-14 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1615436A true CN1615436A (en) | 2005-05-11 |
| CN1268926C CN1268926C (en) | 2006-08-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB028273125A Expired - Fee Related CN1268926C (en) | 2001-12-14 | 2002-04-03 | Water monitoring method using algae |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP2005513438A (en) |
| KR (2) | KR20020023747A (en) |
| CN (1) | CN1268926C (en) |
| AU (1) | AU2002246412A1 (en) |
| WO (1) | WO2003052409A1 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100520365C (en) * | 2006-09-01 | 2009-07-29 | 中国科学院安徽光学精密机械研究所 | Method and device for classified detecting density of phytoplankton under water in site |
| CN102608085A (en) * | 2012-01-09 | 2012-07-25 | 暨南大学 | Method for detecting activity of alga hematoxin and application of method |
| CN103018419A (en) * | 2012-12-18 | 2013-04-03 | 南京大学 | Method for determining risk of toxic pollutants in river water of high content of sand |
| CN103487561A (en) * | 2013-09-17 | 2014-01-01 | 河海大学 | Early warning device and method for identifying sudden water pollution based on biotic population module |
| CN111537484A (en) * | 2020-05-12 | 2020-08-14 | 山东澳联新材料有限公司 | Method for detecting water body pollutants based on fluorescent MOF-plant hybrid |
| CN115691670A (en) * | 2022-08-16 | 2023-02-03 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | River ecosystem health evaluation method based on microbial community specific response |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020023747A (en) * | 2001-12-14 | 2002-03-29 | 김상길 | Water monitoring method using algae |
| KR100734477B1 (en) * | 2006-03-02 | 2007-07-03 | 전북대학교산학협력단 | Toxicity valuation device and method |
| KR100883035B1 (en) | 2007-09-10 | 2009-02-09 | 한국건설기술연구원 | Monitoring device of taste and odor matters(2-mib) in drinking water treatment and method thereof |
| KR101084760B1 (en) | 2009-08-19 | 2011-11-22 | 인천대학교 산학협력단 | Method and kit for evaluating water toxicity using the germination percentage of the spores of marine algae which belongs to Laminariales |
| KR101108614B1 (en) * | 2009-11-06 | 2012-01-31 | 주식회사 파나시아 | A Ballast Water Realtime Monitering Device and Monitering Method using thereof |
| KR101106616B1 (en) * | 2009-12-04 | 2012-01-20 | 한국과학기술연구원 | Method and apparatus for quantitative analysis of the extent of membrane fouling by using fluorescence microorganisms |
| KR20110107652A (en) * | 2010-03-25 | 2011-10-04 | 주식회사 환경바이오 | Portable apparatus for measuring algae |
| KR101224855B1 (en) | 2011-05-31 | 2013-01-22 | (주)제이엠이엔비 | Apparatus for measuring algae using multi wavelength source of light |
| KR101523638B1 (en) * | 2011-11-11 | 2015-05-28 | 인천대학교 산학협력단 | Method for evaluating aquatic ecotoxicity using the growth area change rate of Lemna paucicostata |
| KR101436921B1 (en) * | 2013-02-21 | 2014-09-02 | 주식회사 그린파이오니아 | Method for evaluatig aquatic ecotoxicity usig euglena |
| WO2016208466A1 (en) * | 2015-06-22 | 2016-12-29 | ウシオ電機株式会社 | Method for detecting substance of interest |
| KR101698000B1 (en) | 2015-07-09 | 2017-01-20 | 한국수자원공사 | Online and real time measurement for cyanotoxin and off flavour compounds in water by multipurpose sample injection apparatus |
| CN109270032B (en) * | 2018-08-20 | 2021-08-20 | 杭州腾海科技有限公司 | Water alga on-line monitoring device |
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| JPH03110451A (en) * | 1989-09-25 | 1991-05-10 | Hitachi Elevator Eng & Service Co Ltd | Detecting device for pollution in water storage tank |
| US5130545A (en) * | 1991-04-05 | 1992-07-14 | Lussier Robert R | Video imaging plant management system |
| JP2000241335A (en) * | 1998-12-24 | 2000-09-08 | Fuji Electric Co Ltd | Method and device for counting algae and fine particle |
| JP2000338030A (en) * | 1999-05-27 | 2000-12-08 | Fuji Electric Co Ltd | Method and apparatus for counting blue-green algae, algae and fine particle |
| KR20030003800A (en) * | 2001-07-02 | 2003-01-14 | 정천희 | Apparatus for checking water contamination using water creatures, bacteria and network |
| KR20020023747A (en) * | 2001-12-14 | 2002-03-29 | 김상길 | Water monitoring method using algae |
-
2001
- 2001-12-14 KR KR1020010079492A patent/KR20020023747A/en active Search and Examination
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2002
- 2002-03-21 KR KR10-2002-0015387A patent/KR100466305B1/en not_active IP Right Cessation
- 2002-04-03 JP JP2003553247A patent/JP2005513438A/en active Pending
- 2002-04-03 WO PCT/KR2002/000584 patent/WO2003052409A1/en active Application Filing
- 2002-04-03 AU AU2002246412A patent/AU2002246412A1/en not_active Abandoned
- 2002-04-03 CN CNB028273125A patent/CN1268926C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100520365C (en) * | 2006-09-01 | 2009-07-29 | 中国科学院安徽光学精密机械研究所 | Method and device for classified detecting density of phytoplankton under water in site |
| CN102608085A (en) * | 2012-01-09 | 2012-07-25 | 暨南大学 | Method for detecting activity of alga hematoxin and application of method |
| CN103018419A (en) * | 2012-12-18 | 2013-04-03 | 南京大学 | Method for determining risk of toxic pollutants in river water of high content of sand |
| CN103018419B (en) * | 2012-12-18 | 2015-04-08 | 南京大学 | Method for determining risk of toxic pollutants in river water of high content of sand |
| CN103487561A (en) * | 2013-09-17 | 2014-01-01 | 河海大学 | Early warning device and method for identifying sudden water pollution based on biotic population module |
| CN103487561B (en) * | 2013-09-17 | 2014-07-09 | 河海大学 | Early warning device and method for identifying sudden water pollution based on biotic population module |
| CN111537484A (en) * | 2020-05-12 | 2020-08-14 | 山东澳联新材料有限公司 | Method for detecting water body pollutants based on fluorescent MOF-plant hybrid |
| CN115691670A (en) * | 2022-08-16 | 2023-02-03 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | River ecosystem health evaluation method based on microbial community specific response |
| CN115691670B (en) * | 2022-08-16 | 2023-06-30 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | River ecosystem health evaluation method based on microbial community specific response |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003052409A1 (en) | 2003-06-26 |
| JP2005513438A (en) | 2005-05-12 |
| CN1268926C (en) | 2006-08-09 |
| KR20030051131A (en) | 2003-06-25 |
| KR100466305B1 (en) | 2005-01-14 |
| KR20020023747A (en) | 2002-03-29 |
| AU2002246412A1 (en) | 2003-06-30 |
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