CN1613862A - Adriacin derivative with anti-cancer activity - Google Patents
Adriacin derivative with anti-cancer activity Download PDFInfo
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- CN1613862A CN1613862A CN 200310106919 CN200310106919A CN1613862A CN 1613862 A CN1613862 A CN 1613862A CN 200310106919 CN200310106919 CN 200310106919 CN 200310106919 A CN200310106919 A CN 200310106919A CN 1613862 A CN1613862 A CN 1613862A
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- 201000008261 skin carcinoma Diseases 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 125000005931 tert-butyloxycarbonyl group Chemical group [H]C([H])([H])C(OC(*)=O)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 201000002510 thyroid cancer Diseases 0.000 description 1
- 208000013077 thyroid gland carcinoma Diseases 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 125000004417 unsaturated alkyl group Chemical group 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Abstract
This invention relates to farmiblastina derivatives, shown as formula, wherein: R1=H or OH, R2 and R3= H or C1-4 hydrocarbon, R4=H, C1-40 saturated or unsaturated hydrocarbon or XR5, wherein X=NH or O, R5=C1-40 saturated or unsaturated hydrocarbon, W=O or NH, R6=H, OH, or O(C1-4 hydrocarbon), R7=H or OH. The invention relates to its preparation and use as medicinal active component thereof.
Description
One, technical field
The present invention relates to have the compound shown in the formula (I) of antitumour activity, their preparation method and they are as the application of active constituents of medicine.
Two, technical background
Zorubicin (Doxorubicin) and gentle red Zorubicin Fourth Ring anthraquinone analog compounds such as (Daunorubicin) are to use anticarcinogen very widely, gentle red Zorubicin is to be used for the treatment of one of the most effective medicine of leukemia, Zorubicin is evident in efficacy in the treatment of many solid tumors, comprising: liver cancer, cancer of the stomach, mammary cancer, lung cancer, ovarian cancer and multiple leukemia.Yet, the potential myocardium toxicity make their clinical use be subjected to certain limitation (Doroshow J.H.N.Engl.J.Med.1991,324:843).So far, isolate or synthetic goes out hundreds of their derivatives from nature, attempt to find toxicity low, active high anticarcinogen of new generation, but effect is little.Subsequently, these medicines are connected in monoclonal antibody, thereby reach the purpose of target administration, reduction myocardium toxicity.Such as, Xu Wang etc. are McAb against gastric carcinoma MGb
2Prepare cancer of the stomach specificity IL, insert Zorubicin then, proof IL-Zorubicin can specific identification stomach cancer cell SGL-7901, and with its drug release of carrying to gastric carcinoma cells SGL-7901, thereby improve kill capability (Xu Wang etc., The Fourth Military Medical University's journal, 1992 of its stomach cancer cell SGL-7901 greatly, 13,63-69).Yet monoclonal antibody easily causes immunotoxicity in human body, and its complex manufacturing, and cost is higher.
Three, summary of the invention
The present invention is intended to introduce and can be connected Fourth Ring anthraquinone analog compound and saturated and undersaturated lipid acid by the polypeptide or derivatives thereof of tumour cell and excessive tissue expressed proteins lytic enzyme selective hydrolysis.Thereby the small molecules anticarcinogen of new generation that the present invention developed has the characteristics of two aspects: on the one hand these compounds since tumor tissues to the superabsorbent ability of lipid acid and by the tumor tissues enrichment.On the other hand, these are discharged anticarcinogen by the molecule of enrichment under the effect of aforesaid proteolytic ferment.Thereby reach the dual enrichment function that anticarcinogen is absorbed (target administration) and discharged by selectivity by selectivity, and give the anticancer effect of the small molecules high-efficiency low-toxicity of being invented.
Correspondingly, the present invention relates to the neutrality of following formula (I) expression or the compound of physiology salt form:
R wherein
1Expression H, OH, R
2, R
3Represent H, C respectively
1-4Alkyl, R
4Expression H or C
1-40Saturated or undersaturated alkyl, XR
5, wherein X represents NH or O and R
5Expression C
1-40Saturated or undersaturated alkyl, R
6Expression H, OH, O (C
1-4Alkyl), R
7Expression H, hydroxyl, W represents O, NH.
R
2And R
3Expression C
1-4The alkyl that comprises straight or branched during alkyl, and can be by hydroxyl, COOH, C (O) NH
2, NH
2, NH (C
1-4Alkyl), NHC (O) H, NHC (O) (C
1-4Alkyl), NHC (O) NH
2, NHC (NH) NH
2, O (C
1-4Alkyl) or S (C
1-4Alkyl) replaces.
R
4, R
5Comprise the alkyl of straight or branched when representing saturated or undersaturated alkyl, and can be by OH, COOH, C (O) NH
2, NH
2, NH (C
1-4Alkyl), N (C
1-4Alkyl)
2, NHC (O) H, NHC (O) (C
1-4Alkyl), NHC (O) NH
2, NHC (NH) NH
2, O (C
1-4Alkyl) or S (C
1-4Alkyl) replaces.
The Zorubicin derivative that is suitable in the formula (I) as cancer therapy drug is R
2, R
3Represent H, CH respectively
3, CH (CH
3)
2, CH
2CH
2CH
2CH
3, CH (CH
3) CH
2CH
3, CH
2CH (CH
3)
2Or phenmethyl, be suitable as cancer therapy drug and be wherein R
2Or R
3Represent CH respectively
3, CH (CH
3)
2, CH (CH
3) CH
2CH
3, CH
2CH (CH
3)
2Or those compounds of phenmethyl, be suitable as cancer therapy drug most and be wherein R
2Or R
3Expression CH
3, CH (CH
3)
2, CH (CH
3) CH
2CH
3, CH
2CH (CH
3)
2Those compounds.
Being suitable as the Zorubicin derivative shown in the formula (I) of active constituents of medicine is R
9Those compounds of expression H or hydroxyl, wherein R
9Can be during the expression hydroxyl in cis or trans-isomerism body position.
Being suitable as the compound shown in the formula (I) of active constituents of medicine is R
4Expression C
8-30Those compounds of saturated or undersaturated alkyl, particularly R wherein
4Expression C
12-30Those compounds of saturated or undersaturated alkyl.
Being suitable as the compound shown in the formula (I) of active constituents of medicine is R
4Expression XR
5Shown in the compound those X represent NH or O, and R
5Expression C
8-30Saturated or undersaturated alkyl, being particularly suitable for is R wherein as cancer therapy drug
5Expression C
12-30Those compounds of saturated or unsaturated alkyl.
Be suitable as the compound shown in the formula (I) of active constituents of medicine and be being configured as of dipeptide chain (L, L), (L, D), (D, L), (D, D) those compounds, be suitable as active constituents of medicine most be wherein those dipeptide chains be configured as (L, compound L).
Be suitable as most the compound shown in the formula (I) of active constituents of medicine and be wherein R
1Expression H or OH, R
2Expression 2-propyl group, R
3The expression methyl, R
4Expression C
12-30Saturated or undersaturated alkyl, W represents O or NH, R
6Expression H, OH or OCH
3And R
7Those compounds of expression H or OH.
The method of the compound of preparation claim 1 Chinese style (I) expression is characterized in that acyl chlorides (R
4C (O) Cl) or R
4CO
2The Acibenzolar of H and polypeptide are as dipeptides (H
2NCHR
3C (O) NHCHR
2CO
2H), reaction makes acidylate dipeptides (R
4C (O) HNCHR
3C (O) NHCHR
2CO
2H), be reflected at organic solvent such as methylene dichloride, chloroform, acetone, N, in dinethylformamide, methyl-sulphoxide, glycol dimethyl ether, Virahol, tetrahydrofuran (THF) or the acetonitrile or in the water, or get in the mixture at water and organic solvent and to carry out.Available organic bases or mineral alkali are acid binding agent, available (also can), and pyridines organic bases such as 4-dimethylaminopyridine or 4-(1-pyrryl) pyridine are made catalyzer, and catalyst consumption is between the 1%-20% mole.
Under above-mentioned reaction conditions, the acidylate dipeptides also can be by acyl chlorides (R
4C (O) Cl) or R
4CO
2The Acibenzolar of H and amino acid (H
2NCHR
3CO
2H) reaction makes acylated amino (R
4C (O) HNCHR
3CO
2H), this acylated amino is converted into corresponding acyl chlorides (H then
2NCHR
3COCl) or behind the Acibenzolar again with amino acid (H
2NCHR
2CO
2H) reaction makes acidylate dipeptides (R
4C (O) HNCHR
3C (O) NHCHR
2CO
2H).
This acidylate dipeptides is in the presence of condensing agent (such as carbodiimide or carbonyl dimidazoles etc.); obtain corresponding Acibenzolar with the N-hydroxy-succinamide condensation; this Acibenzolar and Fourth Ring anthraquinone kind anti-cancer drugs thing; it is the compound shown in the formula (II); such as Zorubicin, gentle red Zorubicin, pidorubicin (Epirubicin), Esorubicin, Idarubicin, 5-Iminodaunomycin, reaction obtains the compound of required formula (I) expression.Be reflected at organic solvent such as methylene dichloride, chloroform, acetone, N, carry out in dinethylformamide, methyl-sulphoxide, glycol dimethyl ether, Virahol, tetrahydrofuran (THF) or the acetonitrile.Available (also can) organic bases, especially pyridines organic bases such as 4-dimethylaminopyridine or 4-(1-pyrryl) pyridine is made catalyzer, and catalyst consumption is between the 1%-20% mole.
The compound of formula (I) expression effectively is suitable for disease applicable to all Fourth Ring anthraquinone analog compounds, includes but not limited to intestinal cancer, liver cancer, cancer of the stomach, mammary cancer, lung cancer, ovarian cancer, prostate cancer, cerebral glioma, lymphatic cancer, skin carcinoma, melanoma, thyroid carcinoma, multiple marrow cancer and leukemia.
The compound of formula (I) expression can share with suitable other kind of anticarcinogen, immunostimulant, hormone.
Except the compound that contains at least a formula (I) expression, medicine of the present invention is suitable in the formulation also can contain carrier substance, weighting agent, solvent, thinner, tinting material and/or tamanori.The selection of these assistant agent materials and consumption thereof depends on that medicine is by in gi tract, intravenously, intraperitoneal, intradermal, intramuscular, the nose or mode administration such as topical.
The result who measures with the cell toxicant screening method MTS Assay of the current international practice proves that the compound shown in the Chinese style of the present invention (I) has than highly selective the target cancer, the some of them compound to the activity of human colon's rectal adenocarcinoma cell strain (LoVo Cell) and Zorubicin quite or slightly strong, but different with Zorubicin, the compound shown in the formula (I) is lower to the toxicity of human fibroblast (HFL Cell) and human adenocarcinoma's cell (HeLa Cell).Enforcement of the present invention will provide a kind of PTS of high-efficiency low-toxicity to cancer patient, thereby have high social and economic benefit.
Four, specific embodiments
The abbreviation language
DCC: dicyclohexyl carbodiimide; DCM: methylene dichloride; TFA: trifluoroacetic acid; CDCl
3: deuterochloroform; HCl: hydrogenchloride, Boc: tertbutyloxycarbonyl.
Preparation one: the preparation of oleoyl chloride
Add 0.47mol (150mL) oleic acid in the 500mL there-necked flask, 50 ℃ of heated constant temperature dropwise add phosphorus trichloride 0.69mol (60mL) under the vigorous stirring, be warming up to 80-90 ℃ after adding, and continue reaction 3-5h, have a large amount of yellow dopes to generate.Then reaction mixture being poured into reduces pressure in the 250mL single port flask removes excessive phosphorus trichloride, the debris normal pressure is filtered promptly get oleoyl chloride at last.Method with preparation one can make triacontane acyl chlorides, inferior oleoyl chloride, stearyl chloride, palmityl chloride and lauroyl chloride.
Preparation two: preparation dipeptides L-L-Ala-a word used in person's names propylhomoserin
With Boc-L-L-Ala 9.45g (50mmol), N-hydroxy-succinamide 5.75g (50mmol) with anhydrous glycol dimethyl ether 150ml dissolving after, be cooled to 0 ℃.Add DCC10.3g (50mmol), 0 ℃ of following stirring reaction 1h, at room temperature reaction is spent the night then.Remove by filter the hexamethylene urea, subtract to steam and desolventize glycol dimethyl ether and get thick product.Thick product obtains needle-like crystal Boc-L-L-Ala N-hydroxy-succinamide ester (Boc-L-L-Ala active ester) 10.21g with Virahol 110ml recrystallization twice.Productive rate: 71%, m.p.143-144 ℃.
After a word used in person's names propylhomoserin 2.1g, water 50ml, the abundant stirring and dissolving of sodium bicarbonate 1.5g, the ethylene glycol dimethyl ether solution that adds Boc-L-L-Ala active ester 5g (gained in 1) under the room temperature, behind the about 5h of stirring reaction, subtract to steam and remove glycol dimethyl ether, it is 2 that the aqueous solution is neutralized to the pH value with the 2N aqueous hydrochloric acid, chloroform extraction three times of this aqueous solution, each 50mL, the combined chloroform layer with the saturated sodium-chloride water solution washing once, is used anhydrous sodium sulfate drying more at last.Subtract to steam and remove chloroform, get thick product 3.72g,, get Boc-L-L-Ala-a word used in person's names propylhomoserin 2.23g this thick product re-crystallizing in ethyl acetate.Productive rate: 60%.
Boc-L-L-Ala-a word used in person's names propylhomoserin the 3.6g that adds gained in 2 in the 100mL Erlenmeyer flask, the dichloromethane solution of 25% trifluoroacetic acid, stirring down, room temperature reaction spends the night.Subtract to steam and remove methylene dichloride, get dipeptides L-L-Ala-about 5.1g of L-a word used in person's names propylhomoserin trifluoroacetate.
Preparation three: preparation stearyl dipeptides (stearyl-L-L-Ala-L-a word used in person's names propylhomoserin) (method one)
Add 1.28g dipeptides trifluoroacetate, water 20mL in the 150mL reaction flask, after the stirring and dissolving with the pH value of about 10mL2N aqueous sodium hydroxide solution conditioned reaction system to 9-10.The tetrahydrofuran (THF) 30mL of adding and water equivalent is cooled to 0-5 ℃, adds stearyl chloride 3g, and 0-5 ℃ is reacted 1h under stirring, and reacts under the room temperature and spends the night.Add the 2N aqueous sodium hydroxide solution in the reaction process, the pH value that remains reaction system is 9-10.After reaction finishes, subtract to steam and remove tetrahydrofuran (THF), remainder water solution is regulated pH value to 2 with the 2N aqueous hydrochloric acid, a large amount of white precipitates are separated out.The milk sap that this is white chloroform extraction three times, each 80mL.The combined chloroform layer with saturated sodium chloride aqueous solution washing once, is used anhydrous sodium sulfate drying again.Filter then, subtract to steam and remove chloroform, obtain thick product.Thick product is crossed post.The column chromatography condition is as follows: elutriant is a chloroform: acetate=95: 5, silica gel 100ml. obtains pure product 275mg.
1H?NMR(CDCl
3，ppm)δ7.00(d，1H，J＝8.8Hz)，6.35(d，1H，J＝8.0Hz)，4.629(dq，1H，J＝6.4，8.0Hz)，4.530(dd，1H，J＝4.4，8.8Hz)，2.18-2.28(m，1H)，1.90-2.08(m，2H)，1.55-1.65(m，2H)，1.363(d，3H，J＝6.4Hz)，1.200-1.340(m，28H)，0.915-0.952(m，6H)，0.820-0.910(m，3H)。
Preparation four: preparation oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin
Method with preparation three replaces stearyl chloride can make oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin by oleoyl chloride.
1HNMR(CDCl
3，ppm)δ7.00(d，1H，J＝8.8Hz)，6.35(d，1H，J＝8.0Hz)，5.30-5.45(m，2H)，4.629(dq，1H，J＝6.4，8.0Hz)，4.530(dd，1H，J＝4.4，8.8Hz)，2.18-2.28(m，3H)，1.90-2.08(m，4H)，1.55-1.65(m，2H)，1.363(d，3H，J＝6.4Hz)，1.200-1.340(m，20H)，0.915-0.952(m，6H)，0.820-0.910(m，3H)。
Preparation five: prepare inferior oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin
Method with preparation three replaces stearyl chloride can make inferior oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin by inferior oleoyl chloride.
1H?NMR：(CDCl
3，ppm)δ6.948(d，1H，J＝8.8Hz)，6.122(d，1H，J＝8.0Hz)，5.25-5.42(m，4H)，4.611(dq，1H，J＝6.4，8.0Hz)，4.525(dd，1H，J＝4.6，8.8Hz)，2.740-2.820(m，2H)，2.18-2.28(m，3H)，1.90-2.08(m，4H)，1.55-1.65(m，2H)，1.367(d，3H，J＝6.4Hz)，1.200-1.350(m，14H)，0.915-0.952(m，6H)，0.820-0.910(m，3H)。
Preparation six: preparation palmityl-L-L-Ala-L-a word used in person's names propylhomoserin
Method with preparation three replaces stearyl chloride can make palmityl-L-L-Ala-L-a word used in person's names propylhomoserin by palmityl chloride.
1H?NMR(CDCl
3，ppm)δ7.00(d，1H，J＝8.8Hz)，6.35(d，1H，J＝8.0Hz)，4.629(dq，1H，J＝6.4，8.0Hz)，4.530(dd，1H，J＝4.4，8.8Hz)，2.18-2.28(m，1H)，1.90-2.08(m，2H)，1.55-1.65(m，2H)，1.363(d，3H，J＝6.4Hz)，1.200-1.340(m，24H)，0.915-0.952(m，6H)，0.820-0.910(m，3H)。
Preparation seven: preparation lauroyl-L-L-Ala-L-a word used in person's names propylhomoserin
Method with preparation three replaces stearyl chloride can make lauroyl-L-L-Ala-L-a word used in person's names propylhomoserin by lauroyl chloride.
1H?NMR(CDCl
3，ppm)δ7.00(d，1H，J＝8.8Hz)，6.35(d，1H，J＝8.0Hz)，4.629(dq，1H，J＝6.4，8.0Hz)，4.530(dd，1H，J＝4.4，8.8Hz)，2.18-2.28(m，1H)，1.90-2.08(m，2H)，1.55-1.65(m，2H)，1.363(d，3H，J＝6.4Hz)，1.200-1.340(m，16H)，0.915-0.952(m，6H)，0.820-0.910(m，3H)。
Preparation eight: preparation triacontane acyl-L-L-Ala-L-a word used in person's names propylhomoserin
A, preparation triacontane acyl chlorides
Evaporate to dryness after triacontanoic acid (1 gram) refluxed 6 hours in the 10mL thionyl chloride, vacuum-drying gets waxy solid 1.21 grams after 24 hours.These product will be directly used in the next step.
B, preparation triacontane acyl-L-L-Ala-L-a word used in person's names propylhomoserin
Method with preparation three replaces stearyl chloride can make triacontane acyl-L-L-Ala-L-a word used in person's names propylhomoserin by the triacontane acyl chlorides.
1H?NMR(CDCl
3，ppm)δ7.02(d，1H，J＝8.8Hz)，6.37(d，1H，J＝8.0Hz)，4.63(dq，1H，J＝6.4，8.0Hz)，4.55(dd，1H，J＝4.4，8.8Hz)，2.18-2.30(m，1H)，1.90-2.10(m，2H)，1.55-1.67(m，2H)，1.37(d，3H，J＝6.4Hz)，1.20-1.40(m，52H)，0.91-0.96(m，6H)，0.82-0.93(m，3H)。
Preparation nine: preparation oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin (method two)
Method with preparation three is replaced stearyl chloride and is replaced L-L-Ala-L-a word used in person's names propylhomoserin can make oleoyl-L-L-Ala by the L-L-Ala by oleoyl chloride.
1H?NMR(CDCl
3，ppm)δ6.10(d，1H，J＝6.8Hz)，4.58(dt，1H，J＝6.9，14.0Hz)，2.24(t，2H，J＝7.5Hz)，1.95-2.08(m，4H)，1.55-1.70(m，2H)，1.46(d，2H，7.2Hz)，1.20-1.40(m，20H)，0.820-0.910(m，3H)。
Oleoyl-L-L-Ala (1.1g), N-hydroxy-succinamide (0.33g) are dissolved in the glycol dimethyl ether (35mL), cooling solution is to 0-5 ℃, disposable adding DCC (0.6g), at room temperature reacting 5h. then subtracts to steam and removes glycol dimethyl ether, add ethyl acetate (50mL) in residue, remove by filter the hexamethylene urea, ethyl acetate solution with saturated sodium bicarbonate aqueous solution washing once, the saturated sodium-chloride water solution washing is once used anhydrous sodium sulfate drying.Filter, subtract to steam and remove ethyl acetate, the gained crude product is purified with silicagel column, and (elutriant is a chloroform: acetone=9: 1), get oleoyl-L-L-Ala N-hydroxy-succinamide ester (oleoyl-L-L-Ala Acibenzolar) 1.16g.
Method with preparation three replaces stearyl chloride can make oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin by oleoyl-L-L-Ala Acibenzolar.
The preparation of embodiment one, stearyl-L-L-Ala-L-a word used in person's names propylhomoserin-Zorubicin (compound one)
With the stearyl dipeptides (50mg, 110nmol), (13mg 110nmol) is dissolved among the glycol dimethyl ether 15ml N-hydroxy-succinamide, and cooling solution is to 0-5 ℃, and (23mg 120nmol), at room temperature reacts 5h to disposable adding DCC then.Add in the reaction system Lipodox (58mg, 100nmol), triethylamine 0.2ml, DMF2ml.Reaction is spent the night under the room temperature.Subtract to steam and remove glycol dimethyl ether, in residue, add the 50ml ethyl acetate, remove by filter the hexamethylene urea, ethyl acetate solution washs once with the aqueous hydrochloric acid of 1N, saturated sodium-chloride water solution washs once, combining water layer, with ethyl acetate 30ml back extraction once, the combined ethyl acetate layer is used anhydrous sodium sulfate drying.Filter, subtract to steam and remove ethyl acetate.Separate with TLC at last.Developping agent is a chloroform: acetate: methyl alcohol=96: 2: 2, twice expansion.The colour band of product is taken off, used ethyl acetate: the mixed solvent 30ml of methyl alcohol=1: 1 soaks product under the wash-out.Filter, subtract to steam and remove ethyl acetate and methyl alcohol obtains pure product.
1H?NMR(CDCl
3，ppm)δ13.95(s，1H)，13.24(s，1H)，8.02(d，1H，J＝8.0Hz)，7.77(dd，1H，J＝8.0，8.0Hz)，7.37(d，1H，J＝8.0Hz)，6.93(d，1H，J＝8.4Hz)，6.63(d，1H，J＝8.0Hz)，6.32(d，1H，J＝6.0Hz)，5.49(s，1H)，5.24(s，1H)，4.76(s，2H)，4.629(dq，1H，J＝6.4，8.0Hz)，4.09-4.18(m，3H)，4.05(s，3H)，3.66(s，1H)，3.27(d，1H，J＝18.8Hz)，3.01(d，1H，J＝18.8Hz)，2.33(d，1H，J＝14.4Hz)，2.10-2.23(m，5H)，1.75-1.92(m，2H)，1.50-1.65(m，2H)，1.20-1.40(m，34H)，0.80-0.95(m，9H)。
The preparation of embodiment two, oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin-Zorubicin (compound two)
Preparation method with embodiment one replaces stearyl-L-L-Ala-L-a word used in person's names propylhomoserin to make by oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin.
1H?NMR(CDCl
3，ppm)δ13.94(s，1H)，13.22(s，1H)，8.02(d，1H，J＝7.6Hz)，7.77(dd，1H，J＝7.6，8.4Hz)，7.37(d，1H，J＝8.4Hz)，6.94(d，1H，J＝7.2Hz)，6.62(d，1H，J＝8.0Hz)，6.33(brs，1H)，5.48(s，1H)，5.24-5.42(m，2H)，5.24(s，1H)，4.76(s，2H)，4.62(brs，1H)，4.40-4.50(m，1H)，4.10-4.18(m，3H)，4.05(s，3H)，3.66(s，1H)，3.26(d，1H，J＝18.8Hz)，3.00(d，1H，J＝18.8Hz)，2.75(d，1H，J＝6.4Hz)，2.74(d，1H，J＝6.4Hz)，2.33(d，1H，J＝14.48Hz)，2.10-2.23(m，6H)，1.75-1.92(m，2H)，1.52-1.62(m，2H)，1.44(d，3H，J＝7.2Hz)，1.20-1.40(m，23H)，0.80-0.95(m，9H)。
The preparation of embodiment three, inferior oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin-Zorubicin (compound three)
Preparation method with embodiment one replaces stearyl-L-L-Ala-L-a word used in person's names propylhomoserin to make by inferior oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin.
1H?NMR(CDCl
3，ppm)δ13.95(s，1H)，13.23(s，1H)，8.02(d，1H，J＝7.6Hz)，7.77(dd，1H，J＝7.6，8.4Hz)，7.37(d，1H，J＝8.4Hz)，6.94(d，1H，J＝7.2Hz)，6.62(d，1H，J＝8.0Hz)，6.33(brs，1H)，5.48(s，1H)，5.24-5.42(m，4H)，5.24(s，1H)，4.76(s，2H)，4.62(brs，1H)，4.40-4.50(m，1H)，4.10-4.18(m，3H)，4.05(s，3H)，3.66(s，1H)，3.26(d，1H，J＝18.8Hz)，3.00(d，1H，J＝18.8Hz)，2.75(d，1H，J＝6.4Hz)，2.74(d，1H，J＝6.4Hz)，2.33(d，1H，J＝14.48Hz)，2.10-2.23(m，6H)，1.75-1.92(m，2H)，1.52-1.62(m，2H)，1.20-1.40(m，22H)，0.80-0.95(m，9H)。
The preparation of embodiment four, oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin-gentle red Zorubicin (compound four)
Preparation method with embodiment one is replaced Zorubicin and is replaced stearyl-L-L-Ala-L-a word used in person's names propylhomoserin to make by oleoyl-L-L-Ala-L-a word used in person's names propylhomoserin by gentle red Zorubicin.
1H?NMR(CDCl
3，ppm)δ13.94(s，1H)，13.22(s，1H)，8.02(d，1H，J＝7.6Hz)，7.77(dd，1H，J＝7.6，8.4Hz)，7.37(d，1H，J＝8.4Hz)，6.94(d，1H，J＝7.2Hz)，6.62(d，1H，J＝8.0Hz)，6.33(brs，1H)，5.48(s，1H)，5.24-5.42(m，2H)，5.24(s，1H)，4.62(brs，1H)，4.40-4.50(m，1H)，4.10-4.18(m，3H)，4.05(s，3H)，3.66(s，1H)，3.26(d，1H，J＝18.8Hz)，3.00(d，1H，J＝18.8Hz)，2.75(d，1H，J＝6.4Hz)，2.74(d，1H，J＝6.4Hz)，2.33(d，1H，J＝14.48Hz)，2.10-2.23(m，9H)，1.75-1.92(m，2H)，1.52-1.62(m，2H)，1.44(d，3H，J＝7.2Hz)，1.20-1.40(m，23H)，0.80-0.95(m，9H)
The preparation of embodiment five, palmityl-L-L-Ala-L-a word used in person's names propylhomoserin-Zorubicin (compound five)
Preparation method with embodiment one replaces stearyl-L-L-Ala-L-a word used in person's names propylhomoserin to make by palmityl-L-L-Ala-L-a word used in person's names propylhomoserin.
1H?NMR(CDCl
3，ppm)δ13.95(s，1H)，13.24(s，1H)，8.02(d，1H，J＝8.0Hz)，7.77(dd，1H，J＝8.0，8.0Hz)，7.37(d，1H，J＝8.0Hz)，6.92(d，1H，J＝8.4Hz)，6.63(d，1H，J＝8.0Hz)，6.31(d，1H，J＝6.0Hz)，5.49(s，1H)，5.24(s，1H)，4.76(s，2H)，4.43(dq，1H，J＝6.4，8.0Hz)，4.09-4.18(m，3H)，4.05(s，3H)，3.66(s，1H)，3.26(d，1H，J＝18.8Hz)，3.01(d，1H，J＝18.8Hz)，2.33(d，1H，J＝12.8Hz)，2.05-2.23(m，5H)，1.75-1.92(m，2H)，1.50-1.65(m，2H)，1.20-1.40(m，30H)，0.80-0.95(m，9H)。
The preparation of embodiment six, lauroyl-L-L-Ala-L-a word used in person's names propylhomoserin-Zorubicin (compound six)
Preparation method with embodiment one replaces stearyl-L-L-Ala-L-a word used in person's names propylhomoserin to make by lauroyl-L-L-Ala-L-a word used in person's names propylhomoserin.
1H?NMR(CDCl
3，ppm)δ13.96(s，1H)，13.26(s，1H)，8.04(d，1H，J＝7.2Hz)，7.78(dd，1H，J＝7.8，8.8Hz)，7.38(d，1H，J＝8.8Hz)，6.87(d，1H，J＝8.0Hz)，6.57(d，1H，J＝8.0Hz)，6.24(d，1H，J＝6.0Hz)，5.50(s，1H)，5.27(s，1H)，4.77(s，2H)，4.38-4.45(m，1H)，4.09-4.18(m，3H)，4.07(s，3H)，3.67(s，1H)，3.28(d，1H，J＝18.8Hz)，3.03(d，1H，J＝18.8Hz)，2.34(d，1H，J＝14.0Hz)，2.05-2.25(m，5H)，1.75-1.90(m，2H)，1.52-1.65(m，2H)，1.20-1.40(m，22H)，0.80-0.95(m，9H)。
The preparation of embodiment seven, triacontane acyl-L-L-Ala-L-a word used in person's names propylhomoserin-Zorubicin (compound seven)
Preparation method with embodiment one replaces stearyl-L-L-Ala-L-a word used in person's names propylhomoserin to make by triacontane acyl-L-L-Ala-L-a word used in person's names propylhomoserin.
1H?NMR(CDCl
3，ppm)δ13.95(s，1H)，13.24(s，1H)，8.02(d，1H，J＝8.0Hz)，7.77(dd，1H，J＝8.0，8.0Hz)，7.37(d，1H，J＝8.0Hz)，6.93(d，1H，J＝8.4Hz)，6.63(d，1H，J＝8.0Hz)，6.32(d，1H，J＝6.0Hz)，5.49(s，1H)，5.24(s，1H)，4.76(s，2H)，4.629(dq，1H，J＝6.4，8.0Hz)，4.09-4.18(m，3H)，4.05(s，3H)，3.66(s，1H)，3.27(d，1H，J＝18.8Hz)，3.01(d，1H，J＝18.8Hz)，2.33(d，1H，J＝14.4Hz)，2.10-2.23(m，5H)，1.75-1.92(m，2H)，1.50-1.65(m，2H)，1.16-1.42(m，58H)，0.80-0.95(m，9H)。
Cell toxicant test (MTS Assay)
One, cell strain and reagent
HFL: human fibroblast strain, LoVo: human colon's rectal adenocarcinoma cell strain (ATCC catalog #CCL-229), HeLa: human adenocarcinoma's cell strain (ATCC catalog # CCL-2), SIT solution (SIGMA), RPMI RPMI-1640, phosphoric acid buffer, methyl-sulphoxide (DMSO), MTS solution (Promega), 96 porocyte culture plates, antineoplastic compound.
Two, detect
Above-mentioned cell cultures (RPMI 1640, percent ten bovine serums) a couple of days.Collecting cell, again with cell suspension in RPMI 1640-SIT serum-free medium, grow into 96 orifice plates 20,000 cells in every hole/100 microlitres.Overnight incubation under 5% carbonic acid gas, 37 ℃ of conditions.Second day, methyl-sulphoxide (DMSO) dissolving antineoplastic compound (3-10mM) was as mother liquor.Make positive control with Zorubicin, make negative control with methyl-sulphoxide.By experimental design, the mother liquor dilution is added 96 porocyte culture plates, under 5% carbonic acid gas, 37 ℃ of conditions, cultivated 48 hours.Each hole that adds 20 microlitre MTS solution to 96 porocyte culture plates continues to cultivate 2--4 hour under 5% carbonic acid gas, 37 ℃ of conditions.Read absorbancy in the 490nm wavelength, and be converted to cell survival rate.
Three, result
| Compound | Cell growth half-inhibition concentration (EC 50)(μM) | ||
| The LoVo cell | The HeLa cell | The HFL cell | |
| Methyl-sulphoxide | NA | ?NA | ?NA |
| Zorubicin | 1.0 | ?1.0 | ?1.0 |
| Compound one | ＞10 | ?＞10 | ?NA |
| Compound two | 1.0 | ?＞10 | ?NA |
| Compound three | 1.0 | ?＞10 | ?NA |
| Compound four | 0.9 | ?＞10 | ?NA |
| Compound five | 10 | ?＞10 | ?NA |
| Compound six | ＞10 | ?NA | ?NA |
Annotate: NA-is at 10 μ M non-activities.
Claims (9)
1. the present invention relates to the compound shown in the formula (I) of neutrality or physiology salt form:
R wherein
1Expression H or OH, R
2And R
3Represent H, C respectively
1-4Alkyl and phenmethyl, R
4Expression H, C
1-40Saturated or undersaturated alkyl or XR
5, wherein X represents NH or O, R
5Expression C
1-40Saturated or undersaturated alkyl, W represents O or NH, R
6Expression H, OH or O (C
1-4Alkyl), R
7Expression H or OH.The invention still further relates to the method for compound shown in the preparation formula (I) and they application as active constituents of medicine.
2. the compound shown in claim 1 Chinese style (I) is characterized in that R
1Expression H or OH, R
2And R
3Represent hydrogen atom, methyl, ethyl, propyl group, sec.-propyl, normal-butyl, 2-methyl-propyl, 2-butyl and phenmethyl respectively, R
4Expression C
4-30Saturated or undersaturated alkyl or XR
5, wherein X represents NH or O and R
5Expression C
4-30Saturated or undersaturated alkyl, R
6Expression H, OH or OCH
3, R
7Expression H or OH, W represents O or NH.
3. the compound shown in claim 1 and 2 Chinese styles (I) is characterized in that R
1Expression H or OH, R
2And R
3Represent methyl, sec.-propyl, 2-methyl-propyl, 2-butyl and phenmethyl respectively, R
4Expression C
8-30Saturated or undersaturated alkyl or the expression XR
5, wherein X represents NH or O and R
5Expression C
8-30Saturated or undersaturated alkyl, R
6Expression H, OH or OCH
3, R
7Expression H or OH, W represents O or NH.
4. the compound shown in claim 1,2 and 3 Chinese styles (I) is characterized in that R
1Expression H or OH, R
2Be 2-propyl group, R
3The expression methyl, R
4Expression C
12-30Saturated or undersaturated alkyl, W represents O or NH, R
6Expression H, OH or OCH
3, R
7Expression H or OH.
5. the compound shown in claim 1,2,3 and 4 Chinese styles (I) is characterized in that R
1Expression H or OH, R
2Be 2-propyl group, R
3The expression methyl, R
4Expression C
12-30Saturated or undersaturated alkyl, W represents O, R
6Expression OCH
3, R
7Expression H.
6. prepare the method for compound shown in claim 1,2,3,4 and 5 Chinese styles (I), it is characterized in that acyl chlorides (R
4C (O) Cl) or R
4CO
2The Acibenzolar of H and dipeptides (H
2NCHR
3C (O) NHCHR
2CO
2H) reaction makes acidylate dipeptides (R
4C (O) HNCHR
3C (O) NHCHR
2CO
2H), this acidylate dipeptides and N-hydroxy-succinamide condensation obtain corresponding Acibenzolar, and the compound reaction shown in this Acibenzolar and the formula (II) obtains the compound shown in the required formula (I).
7. prepare the method for compound shown in claim 1,2,3,4 and 5 Chinese styles (I), it is characterized in that acyl chlorides (R
4C (O) Cl) or R
4CO
2The Acibenzolar of H and amino acid (H
2NCHR
3CO
2H) reaction makes acylated amino (R
4C (O) HNCHR
3CO
2H), this acylated amino is converted into corresponding acyl chlorides (H then
2NCHR
3COCl) or behind the Acibenzolar again with amino acid (H
2NCHR
2CO
2H) reaction makes acidylate dipeptides (R
4C (O) HNCHR
3C (O) NHCHR
2CO
2H), this acidylate dipeptides and N-hydroxy-succinamide condensation obtain corresponding Acibenzolar, and the compound reaction shown in this Acibenzolar and the formula (II) obtains the compound shown in the required formula (I).
8. the compound shown in claim 1,2,3,4 and 5 Chinese styles (I) is characterized in that the compound useful as anti-cancer agents thing shown in the formula (I) as the application of active constituents of medicine.
9. the compound shown in claim 1,2,3,4 and 5 Chinese styles (I) is characterized in that as the application of active constituents of medicine the compound shown in the formula (I) can be through stomach by way of reaching through parenteral by way of administration.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101069190A CN100372862C (en) | 2003-11-05 | 2003-11-05 | Adriacin derivative with anti-cancer activity |
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|---|---|
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| CN100372862C CN100372862C (en) | 2008-03-05 |
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ID=34757874
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|---|---|---|---|
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Cited By (8)
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| WO2008089602A1 (en) | 2007-01-18 | 2008-07-31 | Tianjin Hemay Bio-Tech Co. Ltd | The tetracyclic anthraquinones possessing anti-cancer effect |
| CN101353361B (en) * | 2008-08-08 | 2011-06-01 | 中国人民解放军第四军医大学 | Preparation of adriablastina prodrug and use thereof |
| US20120308646A1 (en) * | 2007-01-18 | 2012-12-06 | Hesheng Zhang | Tetracyclic anthraquinones possessing anti-cancer properties |
| CN102875651A (en) * | 2012-09-27 | 2013-01-16 | 亚飞（上海）生物医药科技有限公司 | Anti-tumor target-activated polypeptide doxorubicin and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998010651A1 (en) * | 1996-09-12 | 1998-03-19 | Merck & Co., Inc. | Conjugates useful in the treatment of prostate cancer |
| HRP970566A2 (en) * | 1996-10-30 | 1998-08-31 | Jones Deborah Defeo | Conjugates useful in the treatment of prostate canser |
| JP2004510702A (en) * | 2000-06-14 | 2004-04-08 | メダレックス，インコーポレイティド | Prodrug compounds having isoleucine |
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| JP2010516633A (en) * | 2007-01-18 | 2010-05-20 | 天津和美生物技▲術▼有限公司 | Tetracycline anthraquinone compounds with anticancer activity |
| US20120308646A1 (en) * | 2007-01-18 | 2012-12-06 | Hesheng Zhang | Tetracyclic anthraquinones possessing anti-cancer properties |
| AU2007345099B2 (en) * | 2007-01-18 | 2013-06-13 | Tianjin Hemay Bio-Tech Co. Ltd | The tetracyclic anthraquinones possessing anti-cancer effect |
| CN101353361B (en) * | 2008-08-08 | 2011-06-01 | 中国人民解放军第四军医大学 | Preparation of adriablastina prodrug and use thereof |
| CN102875651A (en) * | 2012-09-27 | 2013-01-16 | 亚飞（上海）生物医药科技有限公司 | Anti-tumor target-activated polypeptide doxorubicin and preparation method and application thereof |
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| US10583175B2 (en) | 2012-11-13 | 2020-03-10 | Adocia | Rapid-acting insulin formulation comprising a substituted anionic compound |
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| US10881716B2 (en) | 2012-11-13 | 2021-01-05 | Adocia | Rapid-acting insulin formulation comprising a substituted anionic compound |
| US11324808B2 (en) | 2012-11-13 | 2022-05-10 | Adocia | Rapid-acting insulin formulation comprising a substituted anionic compound |
| US9795678B2 (en) | 2014-05-14 | 2017-10-24 | Adocia | Fast-acting insulin composition comprising a substituted anionic compound and a polyanionic compound |
| US10525133B2 (en) | 2014-05-14 | 2020-01-07 | Adocia | Aqueous composition comprising at least one protein and one solubilizing agent, preparation thereof and uses thereof |
| US10792335B2 (en) | 2015-11-16 | 2020-10-06 | Adocia | Rapid-acting insulin composition comprising a substituted citrate |
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