CN1607414A - Double telecentric imaging objective for biochip detection - Google Patents
Double telecentric imaging objective for biochip detection Download PDFInfo
- Publication number
- CN1607414A CN1607414A CN 200310100169 CN200310100169A CN1607414A CN 1607414 A CN1607414 A CN 1607414A CN 200310100169 CN200310100169 CN 200310100169 CN 200310100169 A CN200310100169 A CN 200310100169A CN 1607414 A CN1607414 A CN 1607414A
- Authority
- CN
- China
- Prior art keywords
- lens
- telecentric imaging
- imaging object
- test according
- biochip test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Lenses (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A double telecentric imaging objective lens used in biochip test is composed of a front lens set with imaging telecenter and a rear lens set with an objective telecenter, the diaphragm face of a diaphragm unit between the front and rear lens sets is the imaging focal surface of the front lens and the objective focal surface of the rear lens. The front set is composed of five lenses and the rear is composed of four, the Na is 0.28, enhanced is 10, R is 60, the visual field is 100X100 sq mm, front work distance is 50mm, rear work distance is 17.78mn, the conjugate distance is 380mm, which overcomes the shortcoming of poor imaging quality, short front work distance and small imaging visual field.
Description
Technical field
The present invention relates to a kind of two telecentric imaging object lens that are used for biochip test, belong to the image-forming objective lens technical field of bio-chip test device high imaging quality.
Background technology
Genetic chip and protein chip are the biochips that is used for detecting simultaneously the expression of several genes or multiple protein, and it will become topmost molecular diagnosis means in this century.It will not only be used for medical diagnosis, also will be used for fields such as health care, drug screening, environmental protection, agricultural, public security, solution civil disputation and prevention epidemic prevention.
The Mk system of biochip is except that with the fluorescence labeling system, also available chemiluminescent labeling system (Chemi Iuminescence).If adopt the chemiluminescent labeling system, detect with CCD type pick-up unit only.Though existing various chemiluminescence detecting (Chemi Imager) (AlphaInnotech, Kodak, Perkin Elmer) can be used for detecting chemiluminescence type biochip.But because of these pick-up units are not to be specifically designed as biochip test, visual field, the resolution of its detection sensitivity, image quality, imaging multiplying power and object lens are all undesirable, particularly have image defects at 48 holes, 96 holes, 384 holes and 1536 hole biochips, its sensing range is greatly limited.
Summary of the invention
The technical issues that need to address of the present invention are: overcome the deficiencies in the prior art, and a kind of image quality height is provided, simple in structure, bio-chip test device is made the two telecentric imaging object lens that are easy to be used for biochip test.
Technical solution of the present invention is: the two telecentric imaging object lens that are used for biochip test, its characteristics are: be made of with the rear lens group with object space heart far away the front lens group that has as Fang Yuanxin, the light hurdle face of light hurdle spare is picture side's focal plane of front lens between the lens combination of front and back, also is the object space focal plane of rear lens.
Also have outer lens barrel at described front lens group and rear lens group outside, outer lens barrel is formed by connecting with web member by first lens barrel and second lens barrel.
Front lens group is made up of five lens, is added with spacer ring or trim ring between each lens, and rear lens group is made up of four lens, is added with spacer ring or trim ring between each lens.
The numerical aperture of two telecentric imaging object lens (NA)=0.28, multiplying power=10, resolution (R)=60, visual field=100 * 100mm
2, preceding operating distance=50mm, back work distance is from 17.78mm conjugate distance=380mm.
The present invention compared with prior art has the following advantages:
1, be used for the numerical aperture (NA)=0.28 of two telecentric imaging object lens of biochip test, bigger; Imaging resolution (R)=60; The multiplying power of optical system is 10 times.
2, the two telecentric imaging object lens that are used for biochip test are made of 9 lens, and the lens number is few, no a slice gummed part, so objective lens arrangement is simple, has simplified the object lens manufacture craft, has reduced cost of manufacture, has improved the object lens image quality simultaneously.
3, under the wavelength of 460 nanometers, the two telecentric systems of image-forming objective lens optical system have been guaranteed for picture Fang Yuanxin, the object space heart far away.Owing to be two telecentric systems, even therefore biochip and CCD image planes have and depart from and tilt, also can not change the imaging multiplying power of object lens, improve the accuracy of detection of biochip.
4, the two telecentric imaging object lens multiplying powers that are used for biochip test reach 10 times, 5 times of multiplying powers than general image-forming objective lens have improved 2 times, therefore under the situation of same CCD, can detect more large-area biochip, also just reduce the cost of detection of biological chip simultaneously.
In a word, the present invention has overcome that poor, the preceding operating distance of object lens image quality is little in the existing bio-chip test device, imaging viewing field is little, can not become the deficiency of high-quality picture to 48 holes, 96 holes, 384 holes and 1536 hole biochips, have imaging resolution height, the optical system multiplying power is big, simple in structure, cost is low, reduced the characteristics of making the bio-chip test device difficulty significantly.
Description of drawings
Fig. 1 is optics of the present invention and mechanical general construction synoptic diagram.
Embodiment
As shown in Figure 1, the present invention includes optical lens components, spacer ring, the trim ring of outer field lens barrel and internal layer.Skin is a lens barrel, and it is formed by connecting by first lens barrel 3 and second lens barrel, 13 usefulness web members, has high precision to support the flange of all optical lens components of object lens inside and spacer ring thereof, trim ring on second lens barrel 13.Internal layer is nine optical lens components in two lens barrels and spacer ring, the trim ring of fixed optics lens element.From biochip 1 one sides, be first trim ring 2, first lens 23, second lens 22, first spacer ring 4, the 3rd lens 21, second trim ring 5, the 3rd trim ring 7, the 4th lens 20, second spacer ring 8, the 5th lens 19, light hurdle spare 9, the 6th lens 18, the 3rd spacer ring 10, the 7th lens 17, the 4th spacer ring 11, the 8th lens 16, the 5th spacer ring 12, the 9th lens 15 successively.None gummed part of nine optical lens components that this image-forming objective lens comprises.
The optical system that nine optical lens components are formed is two telecentric systems of the picture side heart far away and the object space heart far away, even so biochip and CCD image planes depart from and inclination can not change the image-forming objective lens multiplying power yet.Two groups of lens combination before and after light hurdle spare 9 is divided into nine optical lens components, front lens group is made of first lens 23, second lens 22, the 3rd lens 21, the 4th lens 20, the 5th lens 19; Rear lens group is made of the 6th lens 18, the 7th lens 17, the 8th lens 16, the 9th lens 15 etc.; The light hurdle face of light hurdle spare is picture side's focal plane of front lens group, also is the object space focal plane of rear lens group, also is the pupil plane of optical system simultaneously.Autoluminescence information on the biochip 1 is that two telecentric imaging object lens of 10 times image on the CCD image planes 14 by multiplying power.
Claims (9)
1, the two telecentric imaging object lens that are used for biochip test, it is characterized in that: constitute with rear lens group with object space heart far away by the front lens group that has as Fang Yuanxin, the light hurdle face of light hurdle spare is picture side's focal plane of front lens between the lens combination of front and back, also is the object space focal plane of rear lens.
2, the two telecentric imaging object lens that are used for biochip test according to claim 1 is characterized in that: also have outer lens barrel at described front lens group and rear lens group outside.
3, the two telecentric imaging object lens that are used for biochip test according to claim 2, it is characterized in that: described outer lens barrel is formed by connecting with web member by first lens barrel and second lens barrel.
4, the two telecentric imaging object lens that are used for biochip test according to claim 1 and 2, it is characterized in that: described front lens group is made up of five lens.
5, the two telecentric imaging object lens that are used for biochip test according to claim 4 is characterized in that: can also be added with spacer ring or trim ring between described each lens.
6, the two telecentric imaging object lens that are used for biochip test according to claim 1 and 2, it is characterized in that: described rear lens group is made up of four lens.
7, the two telecentric imaging object lens that are used for biochip test according to claim 5 is characterized in that: can also be added with spacer ring or trim ring between described each lens.
8, the two telecentric imaging object lens that are used for biochip test according to claim 1 and 2, it is characterized in that: the numerical aperture of described pair of telecentric imaging object lens (NA)=0.28, resolution (R)=60, visual field=100 * 100mm2, preceding operating distance=50mm, conjugate distance=380mm.
9, the two telecentric imaging object lens that are used for biochip test according to claim 1 and 2, it is characterized in that: the optical system multiplying power of two telecentric imaging object lens is 10 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310100169 CN1607414A (en) | 2003-10-15 | 2003-10-15 | Double telecentric imaging objective for biochip detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310100169 CN1607414A (en) | 2003-10-15 | 2003-10-15 | Double telecentric imaging objective for biochip detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1607414A true CN1607414A (en) | 2005-04-20 |
Family
ID=34755856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200310100169 Pending CN1607414A (en) | 2003-10-15 | 2003-10-15 | Double telecentric imaging objective for biochip detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1607414A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105403543A (en) * | 2014-09-08 | 2016-03-16 | 立科有限公司 | Fluorescence Imaging Autofocus Systems And Methods |
| CN105954860A (en) * | 2016-05-13 | 2016-09-21 | 西安远心光学系统有限公司 | One-key measurement bi-telecentric lens |
| CN106017540A (en) * | 2016-06-29 | 2016-10-12 | 梧州奥卡光学仪器有限公司 | Micropore detection method based on luminous flux |
| CN106017539A (en) * | 2016-06-29 | 2016-10-12 | 梧州奥卡光学仪器有限公司 | Micropore detection instrument |
| CN113866966A (en) * | 2021-10-11 | 2021-12-31 | 深圳赛陆医疗科技有限公司 | Imaging lens of gene sequencer, gene sequencer and gene sequencing system |
-
2003
- 2003-10-15 CN CN 200310100169 patent/CN1607414A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105403543A (en) * | 2014-09-08 | 2016-03-16 | 立科有限公司 | Fluorescence Imaging Autofocus Systems And Methods |
| CN105954860A (en) * | 2016-05-13 | 2016-09-21 | 西安远心光学系统有限公司 | One-key measurement bi-telecentric lens |
| CN106017540A (en) * | 2016-06-29 | 2016-10-12 | 梧州奥卡光学仪器有限公司 | Micropore detection method based on luminous flux |
| CN106017539A (en) * | 2016-06-29 | 2016-10-12 | 梧州奥卡光学仪器有限公司 | Micropore detection instrument |
| CN113866966A (en) * | 2021-10-11 | 2021-12-31 | 深圳赛陆医疗科技有限公司 | Imaging lens of gene sequencer, gene sequencer and gene sequencing system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103782155B (en) | There is the optical biosensor of multiple sensor region | |
| ES2407968T3 (en) | Procedures and apparatus for confocal imaging | |
| US10352860B2 (en) | Super resolution microscopy | |
| WO2007056490A2 (en) | Fiber optic interrogated microslide, microslide kits and uses thereof | |
| CN1657913A (en) | Microarray chip detection system | |
| CN108577791B (en) | Fluorescence navigation endoscope system and method for enhancing fluorescence imaging sensitivity thereof | |
| JP2013535687A (en) | Cytometry system with a solid numerical aperture increasing lens | |
| CN106248684A (en) | For detecting Optical devices and the method for the internal flaw of transparent substrates | |
| EP3486704A1 (en) | Optical system, image capturing apparatus, distance measuring apparatus, and vehicle-mounted system | |
| JP2018151625A (en) | Tube lens for correcting aberration of continuous spherical surface | |
| CN109406479A (en) | A kind of the real-time fluorescence detection device and its operating method of microreactor | |
| CN1607414A (en) | Double telecentric imaging objective for biochip detection | |
| US20240179427A1 (en) | Photoelectric conversion device and photoelectric conversion system | |
| US6525828B1 (en) | Confocal color | |
| US11262570B2 (en) | Mirror image microscopy for increased collection | |
| US10694944B2 (en) | System and method for enhanced contrast imaging based on detection of different portions of a lateral point-spread of light pattern | |
| US11067781B2 (en) | Microscope and method for localizing fluorescent molecules in three spatial dimensions | |
| KR102644919B1 (en) | Display Panel Inspection System and inspection method thereof | |
| CN211179535U (en) | Micro-droplet double-fluorescence signal detection device | |
| CN209296581U (en) | A kind of real-time fluorescence detection device of microreactor | |
| CN113296253A (en) | Multi-light-sheet light field fluorescence microscopic imaging device | |
| CN109672816A (en) | Image capturing system, matrix image acquisition system and matrix image optical projection system | |
| JPWO2021015838A5 (en) | ||
| JP3959041B2 (en) | Imaging device and luminance distribution measuring device | |
| Otuboah et al. | High-throughput and uniform large field-of-view multichannel fluorescence microscopy with super-thin dichroism for a dPCR gene chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |