CN1605849A - Special equipment for fabricating frozen tissue micro array carrier - Google Patents
Special equipment for fabricating frozen tissue micro array carrier Download PDFInfo
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- CN1605849A CN1605849A CN 200410079248 CN200410079248A CN1605849A CN 1605849 A CN1605849 A CN 1605849A CN 200410079248 CN200410079248 CN 200410079248 CN 200410079248 A CN200410079248 A CN 200410079248A CN 1605849 A CN1605849 A CN 1605849A
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- hand drill
- electronic hand
- frozen tissue
- micro array
- tissue micro
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Abstract
The present invention is apparatus specially for making frozen tissue micro array carrier, which has base body of freeze treated chicken gizzard with holes. The making process includes ultra-low temperature fast freezing fresh chicken gizzard in liquid nitrogen; shaping the fast frozen chicken gizzard in -35~-20 deg.c to form the base body; and drilling holes in the base body at -85~-75 deg.c. The special drilling apparatus may be a reformed drilling machine with base body fixture with coordinates and capable of moving horizontally. The present invention has frozen tissue micro array carrier with low cost, high density of holes and other advantages, and can produce great amount of detected specimens.
Description
The application is that number of patent application is 02109826.3, the applying date is on May 24th, 2002, denomination of invention is divided an application for " frozen tissue micro array carrier, method for making and make specialized equipment ".
Technical field:
The present invention relates to a kind of product in biomedical research, teaching and the clinical analysis, the method for making and special-purpose making apparatus of product of being applied in.Especially a kind of frozen tissue micro array carrier in the cancer genomics, the method for making and special-purpose making apparatus of frozen tissue micro array carrier of being applied in.
Background technology:
At present, cancer has become one of several big killers that threaten the human life, and people are actively seeking to capture method for cancer hardy.The research of cancer genomics promptly is by the gene structure that compares human normal cell, precancerous lesion and malignant cell and the similarities and differences of function, biological function, the science of heredity of examination in tumour generation, evolution changes and development law, in the hope of gene protection and the effective treatment that realizes cancer.Cancer genomics research process then needs utilize hundreds of cancer sample again by genetic chip and biology information technology screening and the closely-related candidate gene of pathogenesis of cancer, and the variation law of each gene of screening is examined.Therefore, press for a kind of under same experiment condition, the experimental technique of each science of heredity index of express-analysis/detections variation situation in a large amount of clinical samples, also tested sample that just need be a large amount of.For addressing this problem, past people has adopted paraffin embedding sample tissue array approach, i.e. purpose zone of under light microscopic, using the microsampling device to obtain all kinds of paraffin-embedded tissues, and it is inserted in another paraffin carrier with the form of array, after the serial section, can obtain more tested sample, to adapt to the detection needs of disparity items.Though the method operation is fairly simple, how the RNA of sample has degraded and only remaining DNA in the paraffin-embedded tissue, and antigenicity also obviously reduces, and easily causes the false negative of experiment, has influenced the accuracy of experiment.For overcoming the deficiency of paraffin embedding sample tissue array approach, someone has adopted another kind of method, be matrix promptly, on matrix, be formed for placing the hole array of sample, form frozen tissue array carrier with OCT material (being recorded on " American Journal of Pathology " delivered Dec calendar year 2001).During use, the specific region in microscopically is selected histotomy separates itself and body portion, inserts in the hole array of carrier, cuts into slices under freezing state, to form a large amount of tested samples.OCT material support method has overcome the deficiency of paraffin embedding sample tissue array approach really, but, since the OCT material when being lower than-20 ℃ fragility higher, and matrix deliquescing when being higher than-10 ℃, be difficult to make the high density arrays carrier, high density can only reach 30 holes/cm.In addition, it is irregular that the OCT material also exists section back array, in the experimentation sample tissue point come off easily, distortion, problem such as overlapping, can not satisfy high-level efficiency, the high accuracy requirement of medical research.
Summary of the invention:
The objective of the invention is in order to solve the existing in prior technology problem, cheap, the highdensity frozen tissue micro array carrier of a kind of cost of manufacture and method, the specialized equipment of making this carrier are provided.
Technical solution of the present invention is: a kind of frozen tissue micro array carrier, and matrix 1 is arranged and be arranged on hole 2 on the matrix 1, described matrix 1 material is fresh chicken's gizzard.
Described matrix 1 is of a size of 0.8cm * 0.8cm * 0.5cm or 0.6cm * 0.6cm * 0.5cm.
Described hole 2 spacings are 0.7mm, and hole 2 diameters are 0.6mm, and the degree of depth is 3～5mm.
Method for making is:
A. with liquid nitrogen fresh chicken's gizzard is carried out super low temperature quick frozen;
B. under-20～-35 ℃ of freezing states, the chicken's gizzard of quick-frozen is shaped to matrix 1;
C. under-75～-85 ℃ of freezing states on matrix 1 drilling bore hole 2.
Special-purpose making apparatus is: be provided with objective table 3, on objective table 3, be fixed with elevating lever 4, elevating lever 4 is provided with screens folder 5, spring 6 and electronic hand drill geometrical clamp 7, electronic hand drill circle presss from both sides surely and is fixed with electronic hand drill 8 and electronic hand drill depression bar 9 on 7, the other end of electronic hand drill depression bar 9 flexibly connects on the screens folder 5, and objective table 3 is provided with the matrix geometrical clamp 10 that indicates coordinate and can move all around.
Be provided with spacing chi 11 between described screens folder 5 and the electronic hand drill geometrical clamp 7.
Also be provided with liquid nitrogen storage poor 12 on the described objective table 3.
Characteristics of the present invention are to be the matrix of frozen tissue micro array carrier with fresh chicken's gizzard, not only cost of manufacture is low, and owing to the densification of chicken's gizzard institutional framework, flexible, fragility is little under cryogenic conditions, therefore can make the high density carrier, high density can reach 64 holes/cm.During use, the experimenter can carry out same index to 64 sample tissue points simultaneously and detect, and has reduced experimenter's working strength, has improved work efficiency; Again because chicken's gizzard is little to the susceptibility that temperature raises, can be at-10 ℃--the carrier that will be equipped with sample in 35 ℃ the temperature range is cut into as thin as a wafer the section of (4 μ m), to produce a large amount of tested samples, satisfy many indexs detections of sample and the many people in the teaching research are used; Chicken's gizzard frozen tissue micro array carrier array after section is very complete, neat, being easy to common microslide adhesion and sample tissue point seldom comes off, can avoid the sample tissue point of prior art to lose, phenomenon such as displacement or crimping, guaranteed the science and the comparability of every testing result.The available dedicated making apparatus is made, and whole manufacture craft is simple.
Description of drawings:
Fig. 1 is the structure cut-away view of embodiment of the invention carrier.
Fig. 2 is the stereographic map of the special-purpose making apparatus of the embodiment of the invention.
Embodiment:
Below in conjunction with description of drawings the specific embodiment of the present invention: get fresh chicken's gizzard, preferably kill and give birth to back about 1 hour chicken's gizzard, place liquid nitrogen to carry out super low temperature quick frozen, then the chicken's gizzard after the quick-frozen is carried out shaping under-20～-35 ℃ freezing state, the chicken's gizzard that is about to quick-frozen is cut into the fritter that is of a size of 0.8cm * 0.8cm * 0.5cm or 0.6cm * 0.6cm * 0.5cm, forms matrix 1.Under-75～-80 ℃ freezing state, getting out diameter on matrix 1 is 0.6mm again, and the degree of depth is the hole array 2 of 3～5mm, and the spacing of aperture 2 is 0.7mm, has so promptly formed frozen tissue micro array carrier as shown in Figure 2.Need use special-purpose making apparatus during boring, the structure of specialized equipment is as shown in Figure 2: promptly be provided with objective table 3, on objective table 3, be fixed with elevating lever 4, elevating lever 4 is provided with screens folder 5, spring 6 and electronic hand drill geometrical clamp 7, be fixed with electronic hand drill 8 and electronic hand drill depression bar 9 on the electronic hand drill geometrical clamp 7, the other end of electronic hand drill depression bar 9 flexibly connects on the screens folder 5, objective table 3 is provided with the matrix geometrical clamp 10 that indicates coordinate and can move all around, can adopt the mobile folder form that indicates scale of microscopically, be provided with spacing chi 11 between screens folder 5 and the electronic hand drill geometrical clamp 7, also be provided with liquid nitrogen storage 12 on the objective table 3.
Concrete operations are adjusted horizontal, vertical adjusting knob of geometrical clamp for matrix 1 is fixed on the matrix geometrical clamp 10, and matrix 1 is fixed on the desired location, adjust the degree of depth that spacing chi 11 is holed with control.Pedal and electric hand drill switch 13 is pressed electronic hand drill depression bar 9, and electronic hand drill 8 can get out satisfactory aperture 2 on matrix 1.Liquid nitrogen storage 12 on the objective table 3 can be placed liquid nitrogen, and potting syrup nitrogen on matrix 1 in good time during boring guarantees that matrix always is in freezing state.
Frozen tissue micro array carrier should be preserved under freezing state.During use, the same with existing OCT material support, sample is inserted in the hole array of carrier, under freezing state, cut into slices, can form a large amount of tested samples.
Claims (1)
1. specialized equipment of making frozen tissue micro array carrier, be provided with objective table (3), on objective table (3), be fixed with elevating lever (4), elevating lever (4) is provided with screens folder (5), spring (6) and electronic hand drill geometrical clamp (7), be fixed with electronic hand drill (8) and electronic hand drill depression bar (9) on the electronic hand drill fixed folder of circle (7), the other end of electronic hand drill depression bar (9) is movably connected on the screens folder (5), it is characterized in that: objective table (3) is provided with the matrix geometrical clamp (10) that indicates coordinate and can move all around, be provided with spacing chi (11) between described screens folder (5) and the electronic hand drill geometrical clamp (7), on objective table (3), also be provided with liquid nitrogen storage (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410079248 CN1605849A (en) | 2002-05-24 | 2002-05-24 | Special equipment for fabricating frozen tissue micro array carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410079248 CN1605849A (en) | 2002-05-24 | 2002-05-24 | Special equipment for fabricating frozen tissue micro array carrier |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021098263A Division CN1187593C (en) | 2002-05-24 | 2002-05-24 | Microarray carrier of frozen tissue and its preparing process and special equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1605849A true CN1605849A (en) | 2005-04-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410079248 Pending CN1605849A (en) | 2002-05-24 | 2002-05-24 | Special equipment for fabricating frozen tissue micro array carrier |
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| Country | Link |
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| CN (1) | CN1605849A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102519773A (en) * | 2012-01-12 | 2012-06-27 | 中国人民解放军第二军医大学 | Liquid nitrogen medium/low-temperature crusher for biological sample |
-
2002
- 2002-05-24 CN CN 200410079248 patent/CN1605849A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102519773A (en) * | 2012-01-12 | 2012-06-27 | 中国人民解放军第二军医大学 | Liquid nitrogen medium/low-temperature crusher for biological sample |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |