CN1583801A - Extracting process for microbiological polysaccharide-hot gel - Google Patents
Extracting process for microbiological polysaccharide-hot gel Download PDFInfo
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- CN1583801A CN1583801A CN 200410041271 CN200410041271A CN1583801A CN 1583801 A CN1583801 A CN 1583801A CN 200410041271 CN200410041271 CN 200410041271 CN 200410041271 A CN200410041271 A CN 200410041271A CN 1583801 A CN1583801 A CN 1583801A
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Abstract
A microbial glycan - thermal gelatin is extracted by: taking yeast liquid of a bacillus foecalis alkaligenes thermal gelatin, centrifuging to get deposition, solving deposition with alkali solution, centrifuging to remove thalli, neutralizing supernatant fluid with hydrochloric acid, washing the neutralized colloidal deposition 1-4 times with deionized water, then centrifugalizing or squeezing to dewater, washing in a little alcohol, drying, pulverizing to obtain crude thermal gelatin. This invention is different from original process for thermal gelatin alkali solution that was deposited extracting by alcohol. It costs low due to decreasing alcohol quantity and gives the different appropriate processing of thermal geletin extracting for crude products and food level products to lower the cost.
Description
Technical field
The extraction process of a kind of microbial polysaccharide-Re gel belongs to technical field of bioengineering.
Background technology
Hot gel claim again to condense polysaccharide, heat coagulates polysaccharide, English Curdlan by name, but when the microorganism of the T.Harada by Osaka, Japan university in 1964, find and studied at the various petroleum components of screening metabolism.Hot gel is a kind of new microbial exocellular polysaccharide, is that main raw material produces through fermentation with the carbohydrate by Bacillus foecalis alkaligenes (Alcaligenes faecalis var.myxogene), and its molecular formula is (C
6H
10O
5)
n, intramolecularly is connected with β-1,3 key by the glucose structural unit.Its chemical structure is as follows:
Hot gel be a kind of under neutrallty condition water-fast polysaccharide.The hot gel of exsiccant is that the fabulous nothing of a kind of flowability is smelt, tasteless white or pale powder solid, and sealing can be stablized preservation for a long time in polyethylene bag, can not lose gelling characteristics.Water insoluble, the pure and mild most of organic solvents of hot gel, but swelling can take place in it in water, can be dissolved in the basic solutions such as certain density NaOH, tertiary sodium phosphate, also be soluble in formic acid, dimethyl sulfoxide (DMSO), saturated urea soln or thiocarbamide and 25% potassiumiodide.The water suspension that heating contains hot gel forms two types gel, a kind ofly be heated to 60 ℃ and be cooled to below 40 ℃ again and form, this gel is hot reversible, and its intensity is lower, therefore character be called as low fixing glue (low-set gel) between the elasticity of the fragility of agar and gelatin; Another is heated to more than 85 ℃ and forms, and is heat irreversible, is heated to 120 ℃ and does not also melt, and this gel structure is solid and have snappiness, and it is called as high fixing glue (high-set gel).Hot gel mainly is based on the characteristic of its second kind of gel in the application of field of food.
Extracting polysaccharide from fermented liquid has a variety of methods, under lab, can remove cell and other insolubles from the fermented liquid of dilution through high speed centrifugation, through dialysis polysaccharide macro-molecular and low-molecular-weight carbohydrate and inorganic salts is separated then.As the needs higher purity, then the ion-exchange of available some form and affinity chromatography are separated polysaccharide and other micro-biomacromolecule.But in industrial production, these methods are unpractical.The research of the feasibility of carrying out from economic viewpoint points out that the cost recovery of polysaccharide product often accounts for the integral part of total cost of production.
Summary of the invention
The extraction process that the purpose of this invention is to provide a kind of microbial polysaccharide-Re gel to reduce the production cost of microbial polysaccharide, makes its market competitiveness stronger.
Technical scheme of the present invention: the fermented liquid of a strain Bacillus foecalis alkaligenes (Alcaligenes faecalis) WX-C12 heat production gel, must precipitate through centrifugal, the gained precipitation is dissolved with alkaline solution, the centrifugal thalline of removing, supernatant liquor neutralizes with hydrochloric acid, in and the gained gelatinous precipitate with deionized water wash 1～2 time, centrifugal then or press dewatering, again with the small amount of ethanol washing, dry, pulverize and promptly get the hot gel product of food grade; Or gained precipitate with deionized water washing 4 times, then with the small amount of ethanol washing, dry, pulverize and promptly get hot gel raw product.Fermented liquid must precipitate through centrifugal, and its technology is 3000～8000rpm, centrifugal 10～30min.The centrifugal gained precipitation of fermented liquid is dissolved with 0.2～0.5N NaOH solution, and used alkali lye volume is 2～5 times of fermented liquid centrifugal sediment volume.The alkaline solution of the hot gel of gained neutralizes with hydrochloric acid, used hydrochloric acid content be in and the required amount of alkali lye, with small amount of ethanol washing, used amount of alcohol is 1～3 times of fermented liquid centrifugal sediment volume.Comprise that drying, all operations temperature of pulverizing are no more than 40 ℃.With the deionized water wash temperature is 0～10 ℃.
Beneficial effect of the present invention: the present invention is divided into raw product and food grade with hot gel product according to different purposes, adopts different process to extract respectively.Therefore raw product extraction process process only washes processing with water without alkali dissolution, and hot gel-forming property is good, but thalline is not removed, somewhat lower purity, but can satisfy extensive use at aspects such as feed, building materials, chemical industry.The method of acid-base neutralisation, washing with alcohol is adopted in the extraction of the hot gel of food grade.The gel-forming property that extracts the hot gel of gained is subjected to the remarkably influenced of alkali lye NaOH concentration, increase along with NaOH concentration, the gel-forming property that extracts the hot gel of gained descends, the present invention is optimized alkali pretreatment, it is proper to determine that the NaOH strength of solution is got 0.2～0.5N, used alkali lye volume is 2～5 times of fermented liquid centrifugal sediment volume, adopts the NaOH solution of this concentration can obtain into that colloidality is good, the purity high product.The hot gel of food grade can be used as the additive of jelly, frozen product, diet food and fast food, beverage, wheaten food and meat product, to improve the characteristic of these food, has been widely used in foodstuffs industry.
The present invention is different from original alkaline solution to hot gel and extracts the technology of hot gel with ethanol sedimentation, can save the ethanol consumption greatly and reduce cost, and can reduce cost more than 50%.Also provided the corresponding extraction process of raw product and the hot gel of food grade according to different purposes, purpose also is in order to reduce cost to greatest extent.
Description of drawings
The extraction schematic flow sheet of the hot gel of Fig. 1 raw product.
The extraction schematic flow sheet of the hot gel of Fig. 2 food grade.
Embodiment
Embodiment 1. hot gel preparation of fermentation liquid
Bacterial strain Bacillus foecalis alkaligenes (Alcaligenes faecalis) WX-C12 Southern Yangtze University biotechnology institute biochemical engineering laboratory preservation and providing, this bacterial strain are open at " Wuxi Light Industry Univ.'s journal " 2001,20 (4), 347～350.
Fermention medium (g/L) glucose 50, yeast extract paste 2, NH
4Cl 3.6, KH
2PO
42, MgSO
47H
2O 0.5, inorganic salt concentrated solution 10mL, pH 7.0 (inorganic salt concentrated solution: FeCl wherein
31g, NaCl 1g, CaCl
21g, MnCl
21g, the 1L deionized water).
Utilize the 9L fermention medium, on 15L Biostat C10-3 mechanical agitator tank, adopt two-stage method (thalli growth is controlled different pH values with the product glue stage) to ferment inoculum size 5%, fermentor tank mixing speed 500rpm, ventilation is 13.5L/min. gauge pressure 0.05MPa, 30 ℃ of leavening temperatures.The NaOH strength of solution of regulating the pH value is 4N, and concentration of hydrochloric acid is 3N.Thalli growth stage pH value is controlled at about 7.0, behind the 18h pH value is transferred to about 5.6, until fermentation ends.
Fermentation initial glucose concentration 50g/L, 40h left and right sides fermented liquid glucose concn drops to 9.7g/L, adds 360g glucose during 44h, and following jar of fermentation culture 90h measures hot gel output 35.9g/L.
Embodiment 2. hot gel preparation of fermentation liquid
Bacterial strain and fermention medium are with embodiment 1.Utilize the 18L fermention medium, inoculum size 5% adopts two-stage method (thalli growth is controlled different pH values with the product glue stage) to ferment on the 25L mechanical agitator tank, fermentor tank mixing speed 300rpm, ventilation is 27L/min, gauge pressure 0.05MPa, 30 ℃ of leavening temperatures.Thalli growth stage pH value is controlled at about 7.0, behind the 21h pH value is transferred to about 5.6, until fermentation ends.
Fermentation initial glucose concentration 50g/L, glucose concn drops to below the 10g/L in the fermentation culture 43h fermented liquid, adds 720g glucose, glucose concn drops to below the 10g/L once more in the 79h fermented liquid, add 720g glucose once more, fermentation culture 110h, hot gel output 43g/L.
The extraction of the hot gel of embodiment 3. food grade
Get the 2L fermented liquid, centrifugal abandoning supernatant, precipitation is dissolved with different N aOH concentration solution respectively, the centrifugal thalline of removing, the alkaline solution of the hot gel of gained neutralizes with hydrochloric acid, in and the gained gelatinous precipitate with deionized water wash 2 times, press dewatering then, again with the small amount of ethanol washing, dry, pulverize and promptly get the hot gel product of food grade.
Drying products is made into 2% suspension liquid, and behind homogeneous, heating 10min becomes glue in 90 ℃ of water-baths, adopts the Texture Analyzer texture analyser of the Micro Stable System of Britain to measure gel-strength.Hot curdlan purity is measured with sulfuric acid-anthrone method.The character of extracting the hot gel of gained under the Different Alkali concentration is as shown in table 1:
Extract the character of the hot gel of gained under the table 1 Different Alkali concentration
| Fermentating liquid volume (L) | Alkali lye NaOH concentration | Dissolve used alkali lye volume (mL) | Ethanol consumption (mL) | Gel-strength (g/cm 2) (2% aqeous suspension) | Purity (in dextrose anhydrous) |
| 2 | 0.2N | 1500 | 500 | 980 | 83％ |
| 2 | 0.3N | 1350 | 500 | 900 | 85％ |
| 2 | 0.5N | 1100 | 500 | 870 | 86％ |
Lower concentration NaOH solution (＜0.2N) dissolving fermented liquid centrifugal sediment, the hot gel purity that extraction obtains lower (unlisted in the table), this is the NaOH solution solution heat gel because of lower concentration, the lysate viscosity ratio is bigger, thalline is not easy centrifugal removing, and it is just lower to extract the hot gel purity of gained.Comprehensive gel-strength and two kinds of indexs of purity, it is proper that the NaOH strength of solution of dissolving fermented liquid centrifugal sediment is got 0.2～0.5N, and used alkali lye volume is 2～5 times of fermented liquid centrifugal sediment volume.Adopt the NaOH solution of this concentration can obtain into that colloidality is good, the purity high product.
The extraction of the hot gel of embodiment 4. raw product
Get the 2L fermented liquid, in 5000rpm, centrifugal 15min obtains precipitation with 4 ℃ of deionized water centrifuge washings four times, and washing with alcohol is used in centrifuge dehydration then, centrifuge dehydration, and 37 ℃ of vacuum-dryings, pulverizing obtains hot gel raw product.Product property is as shown in table 2:
The hot gel raw product of table 2 character
| Fermentating liquid volume (L) | The hot gel output of fermented liquid (g) | Extract dry weight (g) | Gel-strength (g/cm 2) (2% aqeous suspension) | Purity (in dextrose anhydrous) |
| 2 | 71.8 | 72.8 | 1200 | 66.7％ |
This extraction process process is without alkali dissolution, and hot gel molecular triple helical conformation is not destroyed, and therefore hot gel-forming property is good, but thalline is not removed somewhat lower purity.
Claims (6)
1. the extraction process of a microbiological polysaccharide-hot gel is characterized in that precipitating the fermented liquid of a strain Bacillus foecalis alkaligenes (Alcaligenes faecalis) WX-C12 heat production gel through centrifugal,
(a) the gained precipitation is dissolved with alkaline solution, the centrifugal thalline of removing, supernatant liquor neutralizes with hydrochloric acid, in and the gained gelatinous precipitate with deionized water wash 1～2 time, centrifugal then or press dewatering, again with the small amount of ethanol washing, dry, pulverize and promptly get the hot gel product of food grade;
(b) or gained precipitate with deionized water washing 4 times, then with the small amount of ethanol washing, dry, pulverize and promptly get hot gel raw product.
2. extraction process according to claim 1 is characterized in that fermented liquid must precipitate through centrifugal, and its technology is 3000～8000rpm, centrifugal 10～30min.
3. extraction process according to claim 1 is characterized in that (a) described gained wherein precipitates with the dissolving of 0.2～0.5N NaOH solution, and used alkali lye volume is 2～5 times of fermented liquid centrifugal sediment volume.
4. extraction process according to claim 1, the alkaline solution that it is characterized in that the hot gel of (a) described gained wherein neutralizes with hydrochloric acid, used hydrochloric acid content be in and the required amount of alkali lye, with small amount of ethanol washing, used amount of alcohol is 1～3 times of fermented liquid centrifugal sediment volume.
5. extraction process according to claim 1 is characterized in that comprising that drying, all operations temperature of pulverizing are no more than 40 ℃.
6. extraction process according to claim 1 is characterized in that the deionized water wash temperature is 0～10 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410041271 CN1282660C (en) | 2004-06-11 | 2004-06-11 | Extracting process for microbiological polysaccharide-hot gel |
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|---|---|---|---|
| CN 200410041271 CN1282660C (en) | 2004-06-11 | 2004-06-11 | Extracting process for microbiological polysaccharide-hot gel |
Related Child Applications (1)
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|---|---|---|---|
| CNB2006101519266A Division CN100455604C (en) | 2004-06-11 | 2004-06-11 | Extraction of microbiological polysaccharide-hot gel |
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| Publication Number | Publication Date |
|---|---|
| CN1583801A true CN1583801A (en) | 2005-02-23 |
| CN1282660C CN1282660C (en) | 2006-11-01 |
Family
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897073A (en) * | 2014-03-12 | 2014-07-02 | 山东省食品发酵工业研究设计院 | Method for separating and purifying curdlan by use of flocculant |
| CN103897074A (en) * | 2014-03-12 | 2014-07-02 | 山东省食品发酵工业研究设计院 | Method for separating and purifying curdlan by use of membrane process |
| CN104628877A (en) * | 2015-01-20 | 2015-05-20 | 华东师范大学 | Method for crushing and removing microbial cells in curdlan production process |
| CN104745656A (en) * | 2015-03-20 | 2015-07-01 | 江南大学 | Method for directly producing beta-1,3-glucooligosaccharides by virtue of thermal gel fermentation liquor |
| CN106282292A (en) * | 2016-09-20 | 2017-01-04 | 江南大学 | A kind of sevage reagent and the method for ethanol combined extracting water solublity heat setting glue |
| CN108752499A (en) * | 2018-05-08 | 2018-11-06 | 南京农业大学 | A method of separating-purifying can obtain right polysaccharide from microbial fermentation solution |
| CN109517088A (en) * | 2018-11-08 | 2019-03-26 | 东华大学 | A method of curdlan is prepared by raw material of sugar-cane juice |
| CN110183547A (en) * | 2019-06-06 | 2019-08-30 | 泰兴市东圣生物科技有限公司 | One kind can obtain the efficient method for post extraction of right polysaccharide |
| CN113142547A (en) * | 2021-04-20 | 2021-07-23 | 广东药科大学 | Gellan gum/curdlan compound microbial food gum and preparation method thereof |
-
2004
- 2004-06-11 CN CN 200410041271 patent/CN1282660C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897073A (en) * | 2014-03-12 | 2014-07-02 | 山东省食品发酵工业研究设计院 | Method for separating and purifying curdlan by use of flocculant |
| CN103897074A (en) * | 2014-03-12 | 2014-07-02 | 山东省食品发酵工业研究设计院 | Method for separating and purifying curdlan by use of membrane process |
| CN103897074B (en) * | 2014-03-12 | 2016-03-02 | 山东省食品发酵工业研究设计院 | A kind of method utilizing membrane process separation and purification curdlan |
| CN104628877A (en) * | 2015-01-20 | 2015-05-20 | 华东师范大学 | Method for crushing and removing microbial cells in curdlan production process |
| CN104628877B (en) * | 2015-01-20 | 2017-09-19 | 华东师范大学 | The method for removing microbial cell is crushed in a kind of process of producing curdlan |
| CN104745656A (en) * | 2015-03-20 | 2015-07-01 | 江南大学 | Method for directly producing beta-1,3-glucooligosaccharides by virtue of thermal gel fermentation liquor |
| CN104745656B (en) * | 2015-03-20 | 2017-12-26 | 江南大学 | A kind of method that the Portugal's oligosaccharides of β 1,3 is directly produced using curdlan fermentation liquid |
| CN106282292A (en) * | 2016-09-20 | 2017-01-04 | 江南大学 | A kind of sevage reagent and the method for ethanol combined extracting water solublity heat setting glue |
| CN108752499A (en) * | 2018-05-08 | 2018-11-06 | 南京农业大学 | A method of separating-purifying can obtain right polysaccharide from microbial fermentation solution |
| CN109517088A (en) * | 2018-11-08 | 2019-03-26 | 东华大学 | A method of curdlan is prepared by raw material of sugar-cane juice |
| CN110183547A (en) * | 2019-06-06 | 2019-08-30 | 泰兴市东圣生物科技有限公司 | One kind can obtain the efficient method for post extraction of right polysaccharide |
| CN113142547A (en) * | 2021-04-20 | 2021-07-23 | 广东药科大学 | Gellan gum/curdlan compound microbial food gum and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN1282660C (en) | 2006-11-01 |
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