CN1569119A - Dogwood extraction and its preparation method and usage - Google Patents
Dogwood extraction and its preparation method and usage Download PDFInfo
- Publication number
- CN1569119A CN1569119A CN 200410014769 CN200410014769A CN1569119A CN 1569119 A CN1569119 A CN 1569119A CN 200410014769 CN200410014769 CN 200410014769 CN 200410014769 A CN200410014769 A CN 200410014769A CN 1569119 A CN1569119 A CN 1569119A
- Authority
- CN
- China
- Prior art keywords
- extract
- fructus corni
- ethanol
- alcohol
- reduced pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 14
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 240000006766 Cornus mas Species 0.000 title 1
- 239000000284 extract Substances 0.000 claims abstract description 165
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 72
- 210000004369 blood Anatomy 0.000 claims abstract description 58
- 239000008280 blood Substances 0.000 claims abstract description 58
- 206010012601 diabetes mellitus Diseases 0.000 claims abstract description 45
- 238000010828 elution Methods 0.000 claims abstract description 25
- 238000001035 drying Methods 0.000 claims abstract description 20
- 239000003814 drug Substances 0.000 claims abstract description 18
- 239000011347 resin Substances 0.000 claims abstract description 16
- 229920005989 resin Polymers 0.000 claims abstract description 16
- 238000002156 mixing Methods 0.000 claims abstract description 13
- 201000009101 diabetic angiopathy Diseases 0.000 claims description 37
- 230000002829 reductive effect Effects 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 238000010298 pulverizing process Methods 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 16
- 239000012567 medical material Substances 0.000 claims description 15
- 238000001556 precipitation Methods 0.000 claims description 12
- 239000008367 deionised water Substances 0.000 claims description 9
- 229910021641 deionized water Inorganic materials 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 208000007342 Diabetic Nephropathies Diseases 0.000 claims description 7
- 208000033679 diabetic kidney disease Diseases 0.000 claims description 6
- 238000007598 dipping method Methods 0.000 claims description 6
- 238000002481 ethanol extraction Methods 0.000 claims description 6
- 238000005325 percolation Methods 0.000 claims description 6
- 238000010992 reflux Methods 0.000 claims description 5
- 201000002249 diabetic peripheral angiopathy Diseases 0.000 claims description 4
- 206010038491 Renal papillary necrosis Diseases 0.000 claims description 3
- 230000000274 adsorptive effect Effects 0.000 claims description 3
- 239000000469 ethanolic extract Substances 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 230000036285 pathological change Effects 0.000 abstract description 10
- 231100000915 pathological change Toxicity 0.000 abstract description 10
- 210000004204 blood vessel Anatomy 0.000 abstract description 3
- 230000008021 deposition Effects 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 3
- 241000209020 Cornus Species 0.000 abstract 3
- 239000003480 eluent Substances 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 241000700159 Rattus Species 0.000 description 51
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 41
- 239000008103 glucose Substances 0.000 description 41
- 210000002966 serum Anatomy 0.000 description 29
- 238000002474 experimental method Methods 0.000 description 27
- HAMNKKUPIHEESI-UHFFFAOYSA-N aminoguanidine Chemical group NNC(N)=N HAMNKKUPIHEESI-UHFFFAOYSA-N 0.000 description 24
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 17
- 230000000694 effects Effects 0.000 description 17
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 17
- 229960001052 streptozocin Drugs 0.000 description 17
- YTZSBJLNMIQROD-SFBCHFHNSA-N Morroniside Chemical compound O([C@@H]1OC=C([C@H]2C[C@H](O)O[C@@H](C)[C@H]21)C(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YTZSBJLNMIQROD-SFBCHFHNSA-N 0.000 description 16
- 239000000243 solution Substances 0.000 description 16
- 238000011552 rat model Methods 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 241001465754 Metazoa Species 0.000 description 9
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 9
- 229940079593 drug Drugs 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- PLSAJKYPRJGMHO-UHFFFAOYSA-N ursolic acid Natural products CC1CCC2(CCC3(C)C(C=CC4C5(C)CCC(O)C(C)(C)C5CCC34C)C2C1C)C(=O)O PLSAJKYPRJGMHO-UHFFFAOYSA-N 0.000 description 9
- 210000003462 vein Anatomy 0.000 description 9
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 8
- YTZSBJLNMIQROD-UHFFFAOYSA-N (4aS)-1c-beta-D-glucopyranosyloxy-6xi-hydroxy-8t-methyl-(4ar,8ac)-5,6,8,8a-tetrahydro-1H,4aH-pyrano[3,4-c]pyran-4-carboxylic acid methyl ester Natural products C12C(C)OC(O)CC2C(C(=O)OC)=COC1OC1OC(CO)C(O)C(O)C1O YTZSBJLNMIQROD-UHFFFAOYSA-N 0.000 description 8
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 230000001225 therapeutic effect Effects 0.000 description 8
- 210000002700 urine Anatomy 0.000 description 8
- IXZISFNWUWKBOM-ARQDHWQXSA-N fructosamine Chemical compound NC[C@@]1(O)OC[C@@H](O)[C@@H](O)[C@@H]1O IXZISFNWUWKBOM-ARQDHWQXSA-N 0.000 description 7
- 239000007924 injection Substances 0.000 description 7
- 238000002347 injection Methods 0.000 description 7
- 230000002262 irrigation Effects 0.000 description 7
- 238000003973 irrigation Methods 0.000 description 7
- AMBQHHVBBHTQBF-UOUCRYGSSA-N loganin Chemical compound O([C@@H]1OC=C([C@H]2C[C@H](O)[C@H](C)[C@H]21)C(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O AMBQHHVBBHTQBF-UOUCRYGSSA-N 0.000 description 7
- 210000002784 stomach Anatomy 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- WCGUUGGRBIKTOS-GPOJBZKASA-N (3beta)-3-hydroxyurs-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C WCGUUGGRBIKTOS-GPOJBZKASA-N 0.000 description 6
- MIJYXULNPSFWEK-GTOFXWBISA-N 3beta-hydroxyolean-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C MIJYXULNPSFWEK-GTOFXWBISA-N 0.000 description 6
- JKLISIRFYWXLQG-UHFFFAOYSA-N Epioleonolsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4CCC3C21C JKLISIRFYWXLQG-UHFFFAOYSA-N 0.000 description 6
- YBRJHZPWOMJYKQ-UHFFFAOYSA-N Oleanolic acid Natural products CC1(C)CC2C3=CCC4C5(C)CCC(O)C(C)(C)C5CCC4(C)C3(C)CCC2(C1)C(=O)O YBRJHZPWOMJYKQ-UHFFFAOYSA-N 0.000 description 6
- MIJYXULNPSFWEK-UHFFFAOYSA-N Oleanolinsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C MIJYXULNPSFWEK-UHFFFAOYSA-N 0.000 description 6
- 239000002253 acid Substances 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 229940100243 oleanolic acid Drugs 0.000 description 6
- 238000000247 postprecipitation Methods 0.000 description 6
- 230000003449 preventive effect Effects 0.000 description 6
- HZLWUYJLOIAQFC-UHFFFAOYSA-N prosapogenin PS-A Natural products C12CC(C)(C)CCC2(C(O)=O)CCC(C2(CCC3C4(C)C)C)(C)C1=CCC2C3(C)CCC4OC1OCC(O)C(O)C1O HZLWUYJLOIAQFC-UHFFFAOYSA-N 0.000 description 6
- 230000002207 retinal effect Effects 0.000 description 6
- 229940096998 ursolic acid Drugs 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 210000003725 endotheliocyte Anatomy 0.000 description 5
- 230000002792 vascular Effects 0.000 description 5
- AMBQHHVBBHTQBF-UHFFFAOYSA-N Loganin Natural products C12C(C)C(O)CC2C(C(=O)OC)=COC1OC1OC(CO)C(O)C(O)C1O AMBQHHVBBHTQBF-UHFFFAOYSA-N 0.000 description 4
- 239000003463 adsorbent Substances 0.000 description 4
- 210000002469 basement membrane Anatomy 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 210000003038 endothelium Anatomy 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 210000002381 plasma Anatomy 0.000 description 4
- 210000003556 vascular endothelial cell Anatomy 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 208000017442 Retinal disease Diseases 0.000 description 3
- 206010038923 Retinopathy Diseases 0.000 description 3
- VSJGJMKGNMDJCI-ZASXJUAOSA-N Sweroside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](C=C)[C@H](CCOC2=O)C2=CO1 VSJGJMKGNMDJCI-ZASXJUAOSA-N 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- MUCRYNWJQNHDJH-OADIDDRXSA-N Ursonic acid Chemical compound C1CC(=O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C MUCRYNWJQNHDJH-OADIDDRXSA-N 0.000 description 3
- MUCRYNWJQNHDJH-UHFFFAOYSA-N Ursonic acid Natural products C1CC(=O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)C(C)C5C4=CCC3C21C MUCRYNWJQNHDJH-UHFFFAOYSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 210000005252 bulbus oculi Anatomy 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 210000000585 glomerular basement membrane Anatomy 0.000 description 3
- 230000036252 glycation Effects 0.000 description 3
- 229930182470 glycoside Natural products 0.000 description 3
- 150000002338 glycosides Chemical class 0.000 description 3
- 201000001421 hyperglycemia Diseases 0.000 description 3
- 150000008145 iridoid glycosides Chemical class 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 230000036542 oxidative stress Effects 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- VSJGJMKGNMDJCI-QXSNVGMTSA-N sweroside Natural products OC[C@H]1O[C@H](O[C@@H]2OC=C3[C@@H](CCOC3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O VSJGJMKGNMDJCI-QXSNVGMTSA-N 0.000 description 3
- 241000759833 Cornus officinalis Species 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 206010020880 Hypertrophy Diseases 0.000 description 2
- 208000004880 Polyuria Diseases 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- ROAYSRAUMPWBQX-UHFFFAOYSA-N ethanol;sulfuric acid Chemical compound CCO.OS(O)(=O)=O ROAYSRAUMPWBQX-UHFFFAOYSA-N 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- 208000030159 metabolic disease Diseases 0.000 description 2
- 230000004089 microcirculation Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 210000003668 pericyte Anatomy 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 206010036067 polydipsia Diseases 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 229940002612 prodrug Drugs 0.000 description 2
- 239000000651 prodrug Substances 0.000 description 2
- 210000001525 retina Anatomy 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 208000037157 Azotemia Diseases 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 235000002687 Caesalpinia echinata Nutrition 0.000 description 1
- 102000016289 Cell Adhesion Molecules Human genes 0.000 description 1
- 108010067225 Cell Adhesion Molecules Proteins 0.000 description 1
- 208000012239 Developmental disease Diseases 0.000 description 1
- 206010012689 Diabetic retinopathy Diseases 0.000 description 1
- 208000004132 Diffuse mesangial sclerosis Diseases 0.000 description 1
- 238000011891 EIA kit Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- 206010022530 Intercapillary glomerulosclerosis Diseases 0.000 description 1
- ZUKLFFYDSALIQW-MSUKCBDUSA-N Iridoid glycoside Chemical compound [H][C@]12CC[C@H](C(O)=O)[C@@]1([H])[C@H](OC1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)OC=C2 ZUKLFFYDSALIQW-MSUKCBDUSA-N 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 241000127464 Paubrasilia echinata Species 0.000 description 1
- 206010040925 Skin striae Diseases 0.000 description 1
- 206010043189 Telangiectasia Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108010093894 Xanthine oxidase Proteins 0.000 description 1
- 102100033220 Xanthine oxidase Human genes 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 210000002376 aorta thoracic Anatomy 0.000 description 1
- 210000002565 arteriole Anatomy 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000008721 basement membrane thickening Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000011095 buffer preparation Methods 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 230000002851 endotheliumprotective effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- SRCZQMGIVIYBBJ-UHFFFAOYSA-N ethoxyethane;ethyl acetate Chemical compound CCOCC.CCOC(C)=O SRCZQMGIVIYBBJ-UHFFFAOYSA-N 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000001434 glomerular Effects 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 210000004276 hyalin Anatomy 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 229930182489 iridoid glycoside Natural products 0.000 description 1
- 230000000302 ischemic effect Effects 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000000713 mesentery Anatomy 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000004279 orbit Anatomy 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 208000009056 telangiectasis Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- -1 triterpene acids Chemical class 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 102000003390 tumor necrosis factor Human genes 0.000 description 1
- 208000009852 uremia Diseases 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 210000004127 vitreous body Anatomy 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims (15)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200410014769 CN1259068C (en) | 2004-04-28 | 2004-04-28 | Dogwood extraction and its preparation method and usage |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200410014769 CN1259068C (en) | 2004-04-28 | 2004-04-28 | Dogwood extraction and its preparation method and usage |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1569119A true CN1569119A (en) | 2005-01-26 |
CN1259068C CN1259068C (en) | 2006-06-14 |
Family
ID=34478587
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 200410014769 Expired - Lifetime CN1259068C (en) | 2004-04-28 | 2004-04-28 | Dogwood extraction and its preparation method and usage |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1259068C (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100435810C (en) * | 2005-08-15 | 2008-11-26 | 江苏吴中苏药医药开发有限责任公司 | Dogwood fruit extract and its preparation process |
EP1773356A4 (en) * | 2004-07-29 | 2010-01-13 | Univ Michigan State | Insulin secretion by anthocyanins and anthocyanidins |
EP1771188A4 (en) * | 2004-07-29 | 2010-04-21 | Univ Michigan State | Methods and compositions for the treatment of obesity, insulin related diseases and hypercholesterolemia |
CN101810335A (en) * | 2010-04-30 | 2010-08-25 | 西南大学 | Method for preparing health-care foods with different activities by comprehensively using dogwoods |
CN102389162A (en) * | 2011-08-04 | 2012-03-28 | 深圳烟草工业有限责任公司 | Method for preparing cornus officinalis extract, application in cigarettes and cigarette |
CN103054890A (en) * | 2012-12-12 | 2013-04-24 | 浙江中医药大学 | Application of cornuside in preparing pharmaceuticals used to cure diabetic nephropathy |
CN104523809A (en) * | 2014-12-09 | 2015-04-22 | 河南中医学院 | Preparation method of common macrocarpium fruit pigment |
CN108562682A (en) * | 2018-04-01 | 2018-09-21 | 李�远 | A kind of thin-layer identification method of dogwood fruit granule and wine-prepared fructus corni granule |
CN115040558A (en) * | 2022-06-25 | 2022-09-13 | 浙江双糖生物科技有限公司 | Natural extract for reducing blood sugar and application thereof in preparing medicine products for treating diabetes and metabolic syndrome |
-
2004
- 2004-04-28 CN CN 200410014769 patent/CN1259068C/en not_active Expired - Lifetime
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1773356A4 (en) * | 2004-07-29 | 2010-01-13 | Univ Michigan State | Insulin secretion by anthocyanins and anthocyanidins |
EP1771188A4 (en) * | 2004-07-29 | 2010-04-21 | Univ Michigan State | Methods and compositions for the treatment of obesity, insulin related diseases and hypercholesterolemia |
CN100435810C (en) * | 2005-08-15 | 2008-11-26 | 江苏吴中苏药医药开发有限责任公司 | Dogwood fruit extract and its preparation process |
CN101810335A (en) * | 2010-04-30 | 2010-08-25 | 西南大学 | Method for preparing health-care foods with different activities by comprehensively using dogwoods |
CN101810335B (en) * | 2010-04-30 | 2012-07-25 | 西南大学 | Method for preparing health-care foods with different activities by comprehensively using dogwoods |
CN102389162A (en) * | 2011-08-04 | 2012-03-28 | 深圳烟草工业有限责任公司 | Method for preparing cornus officinalis extract, application in cigarettes and cigarette |
CN103054890A (en) * | 2012-12-12 | 2013-04-24 | 浙江中医药大学 | Application of cornuside in preparing pharmaceuticals used to cure diabetic nephropathy |
CN104523809A (en) * | 2014-12-09 | 2015-04-22 | 河南中医学院 | Preparation method of common macrocarpium fruit pigment |
CN104523809B (en) * | 2014-12-09 | 2018-08-07 | 河南中医学院 | A kind of preparation method of Fructus Corni pigment |
CN108562682A (en) * | 2018-04-01 | 2018-09-21 | 李�远 | A kind of thin-layer identification method of dogwood fruit granule and wine-prepared fructus corni granule |
CN115040558A (en) * | 2022-06-25 | 2022-09-13 | 浙江双糖生物科技有限公司 | Natural extract for reducing blood sugar and application thereof in preparing medicine products for treating diabetes and metabolic syndrome |
Also Published As
Publication number | Publication date |
---|---|
CN1259068C (en) | 2006-06-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1669573A (en) | Chinese medicine preparation for treating cardiovascular and cerebrovascular diseases and preparing process thereof | |
CN101040915A (en) | Method for preparing a Shuanhuanglian injection and the component detecting method | |
CN1768854A (en) | Chinese medicinal capsule with spleen-supplementing, intestine-benefiting function | |
CN1569119A (en) | Dogwood extraction and its preparation method and usage | |
CN101058578A (en) | Dextrorotation deoxidization tylophorinine, preparation method thereof, pharmaceutical composition and use thereof | |
CN1569120A (en) | Effective portion extraction of dogwood and its preparation method and usage | |
CN101028323A (en) | Chinese-medicinal composition for treating cough asthma | |
CN1569867A (en) | Preparation method and use of skunk bush extract morroniside | |
CN1620305A (en) | Method of inducing apoptosis and compositions therefor | |
CN1679648A (en) | Mailuoning injection and its preparation and quality control | |
CN1174973C (en) | Schizonepeta lactone and its extraction process and use | |
CN1562064A (en) | New use of saponin compound for treating cardiovascular disease | |
CN1511583A (en) | Chinese rose extract and its preparing method and use | |
CN1569884A (en) | Method for preparing astragaloside and its use in preparation of drug for preventing and treating diabetic nephropathy | |
CN1823887A (en) | Quality control method of injection preparation used for treating tumour | |
CN1268323C (en) | Rhodiola sacra soft capsule and its preparation | |
CN1206236C (en) | Manyprickle acanthopanax general saponin extractive and its medicinal composition | |
CN1188406C (en) | Cattail pollen extract and preparation process and use thereof | |
CN1682970A (en) | Method for preparing herb of sowthistle leaf Ixeris injection | |
CN1194743C (en) | Chinese medicinal composition for treating atrophic arthritis, preparing method and quality controlling method thereof | |
CN101040934A (en) | Medicine compound made of haw leaf and rhodiola | |
CN1251693C (en) | Chinese-western medicine compound preparation and its preparing method | |
CN1923229A (en) | Pharmaceutical composition comprising notoginseng extract, Danshen extract and puerarin | |
CN1123663A (en) | Composition for curing angicardiopathy and its production and usage | |
CN1269491C (en) | Isatis root extract composition, its preparation method and application |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address |
Address after: 211100 building 6, block A, Gao Hu Road, Jiangning Development Zone, Jiangning District, Jiangsu, Nanjing, 105 Patentee after: Jiangsu Zeukov Pharmaceutical S. & T. Inc. Address before: 211100 A block, No. 208, Xin Xin Road, Jiangning economic and Technological Development Zone, Nanjing District, Jiangsu, 6 Patentee before: Xiangxi ZeuKov New Drug Finger Print Development Co.,Ltd. |
|
CP03 | Change of name, title or address | ||
TR01 | Transfer of patent right |
Effective date of registration: 20171013 Address after: 222001 Jiangning industrial city, Lianyungang economic and Technological Development Zone, Jiangsu Patentee after: JIANGSU KANION PHARMACEUTICAL Co.,Ltd. Address before: 211100 building 6, block A, Gao Hu Road, Jiangning Development Zone, Jiangning District, Jiangsu, Nanjing, 105 Patentee before: Jiangsu Zeukov Pharmaceutical S. & T. Inc. |
|
TR01 | Transfer of patent right | ||
CX01 | Expiry of patent term |
Granted publication date: 20060614 |
|
CX01 | Expiry of patent term |