CN1562420A - Method for restoring soil polluted by polycyclic aromatic hydrocarbon through plants - Google Patents

Abstract

A method for improving the polycyclic arylhydrocarbon polluted soil by use of the surfactant synergitis plant includes such steps as planting the perennial ryegrass or the plant 'Hongsanye' in the polluted soil by the density of 200-250 plants/sq.m, applying the solution of non-ionic surfactant polyoxyvinyl anhydrosorbitol monooleate or laurinol polyoxyvinyl ether when the height of said plant is 10-12 cm, growing for 50-60 days, harvesting, drying and burning at other position.

Description

The surfactant Synergistic plant is repaired the method for polycyclic aromatic hydrocarbon pollution

Technical field

The present invention relates to contaminated environment recovery technique field, relate in particular to the method that a kind of surfactant Synergistic plant is repaired polycyclic aromatic hydrocarbon pollution.

Background technology

Soil is one of the mankind's most important natural resources of depending on for existence.But owing to atmospheric sedimentation (combustion matchmaker and motor vehicle exhaust emission), sewage irrigation, solid waste landfill seepage, oilfield exploitation etc., the soil organic contamination is on the rise.Polycyclic aromatic hydrocarbon organic pollutions such as (PAHs) has " three cause " effect mostly, can move in soil-crop system, and causes the underground water secondary pollution, jeopardizes agricultural product security and health [1].Therefore, keep soil environment safety significant.The soil of organic contaminations such as reparation PAHs is one of current domestic and international soil and the common technical problem paid close attention to of environmental area, but does not almost have the practical technique of organic contaminations such as economy, effective for repairing P in soil AHs so far.

The recovery technique of organic polluted soil mainly contains chemistry reparation, biological restoration (microorganism reparation, phytoremediation, plant-microorganism are united reparation), the chemical reparation [2] that combines with biofacies.Chemistry is repaired synergy reagent such as normally injecting surfactant in the soil body, improve the flowability of organic pollutions such as PAHs and the soil body of moving out, or form anelasticity and the stability that organic clay improves pollutant, reduce the ability that its migration enters other surrounding medium; But chemistry is repaired the secondary pollution easily cause surrounding mediums such as underground water, and the disposal cost costliness, unsuitable large-scale application [3].It is the obligate microbial degradation soil organic pollutant that utilizes screening, domestication that microorganism is repaired, and realizes the purpose of repairing; But obligate microorganism or the genetic engineering bacterium introduced in a large number in the soil have potential ecological risk [4].The phytoremediation contaminated soil is one of economy, valid approach, but present research work mainly concentrates on screening or cultivation hyperaccumulative plant, with the heavy metal pollution of rehabilitating soil.People also wish to pollute with plant soil restoration PAHs, have studied the influence to remediation efficiency such as floristics, pollutant character, soil types, and its repair is embodied in two aspects: plant is to the absorption and accumulation and the degraded of organic pollutions such as PAHs; Particularly secretions from plant roots promotes soil indigenous microorganism degradation of contaminant, but the absorption and accumulation of plant own is to the contribution rate of phytoremediation relatively low (but still not have at present to find the plant of organic pollutions such as can the tired PAHs of ultraproduct), and how improving the efficient that plant absorbing accumulates organic pollutions such as P in soil AHs is one of key of phytoremediation [5].The reparation that chemistry combines with biofacies is to utilize surfactant to the solubilising wash-out effect of organic pollution in the soil and the influence of bioavailability, improves the efficient of soil organic contamination biological restoration; Studied the synergistic effect of surfactant to the organic contamination of microorganism rehabilitating soil; But can to solve the key technical problem in the plant soil restoration organic contamination, still not report both at home and abroad so far with the efficient of surfactant fortification of plants renovation of organic pollution soil.

[1] Liu Shiliang, Luo Yongming, Cao Zhihong, the Dinke is strong, the microorganism and the plant combined reparation progress of Jiang .2002. of army of elder generation polycyclic aromatic hydrocarbon pollution. soil .34 (5): 257-265

[2] chemistry and the biological restoration of .1999. soil and underground water organic contamination among the Zhu Li. environmental science progress .7 (2): 65-71

[3]Zhou，M.；Rhue，R.D.2000.Screening?commercial?surfactants?suitable?for?remediatingDNAPL?source?zones?by?solubilization.Environ.Sci.Technol.34：1985-1990

[4]Wilson，S.C.；Jones，K.C.1993.Bioremediation?of?soil?contaminated?with?polycyclicaromatic?hydrocarbons(PAHs)：a?review.Environ.Pollut.81：229-249

[5]Gao，Y.Z.；Zhu，L.Z.2003.Phytoremediation?and?its?models?for?organic?contaminatedsoils.J?Environ?Sci.15：302-310

Summary of the invention

The purpose of this invention is to provide a kind of surfactant Synergistic plant and repair the method for polycyclic aromatic hydrocarbon pollution.

The step of method is as follows:

1) plant rye grass (Lolium multiflorum Lam) or red clover (Trifolium pretense L.) plant in polycyclic aromatic hydrocarbons contaminated soil, the planting density of rye grass or red clover is every square metre 200～250 strain;

When 2) treating that plant grows to plant height 10～12cm, in soil, apply non-ionic surfactant polyoxyethylene (20) anhydrous sorbitol list olein or lauryl alcohol polyethylene glycol oxide (23) ethereal solution, the amount of application 15～150g of non-ionic surface active agent in every square metre of soil.

3) after growing 50～60 days, gather in the crops plant, dry and be transferred to other local concentrating and burn, get final product.

Advantage of the present invention is that the non-ionic surface active agent that employing is easily degraded is a synergy reagent, has avoided the pollution of external source chemical reagent to soil; Technical matters is simple, and cost is low; Repairing effect to organic polluted soils such as polycyclic aromatic hydrocarbons is good; Be applicable to the improvement of large tracts of land organic polluted soil.

Description of drawings

Accompanying drawing is polyoxyethylene (a 20) anhydrous sorbitol list olein sowing amount when being 1000mg/kg soil, the residual concentration and the time relation schematic diagram of pyrene in the pyrene contaminated soil of plantation rye grass.

The specific embodiment

(Surfactant-enhanced phytoremediation SEPR) is the solubilization that utilizes surfactant to the reparation of surfactant Synergistic plant, and the organic pollution that is adsorbed on the soil is desorbed, and is solubilized into the soil liquid; And improve the bioavailability of organic pollution, promote plant absorbing accumulation and microbial degradation organic pollution; Improve the phytoremediation efficient of soil organic contamination thus.

Embodiment 1: the pyrene contaminated soil that contains pyrene 105mg/kg was loaded in the basin alms bowl after grinding the 3mm sieve, and balance is 4 days under 50% field capacity; To the basin alms bowl, the management method according to common crops behind plant survival manages with rye grass or red clover seedling replanting; When treating that plant grows to plant height 10～12cm, in soil, apply non-ionic surface active agent Tween80, i.e. polyoxyethylene (20) anhydrous sorbitol list olein solution, amount of application 100mg (concentration * volume) in every kg soil; Gather soil and plant sample after 50 days, and analyze the content of pyrene in the soil, estimate repairing effect.

Cross 20 mesh sieves after the soil sample collection, take by weighing 2g in the glass centrifuge tube, add the 2g anhydrous Na ₂SO ₄, abundant mixing; After adding the 10mL carrene, ultrasonic extraction; Extract Rotary Evaporators evaporate to dryness is analyzed with high performance liquid chromatograph after the 2ml methanol constant volume.

After 50 days, the clearance of pyrene＞91% in the soil.

Embodiment 2: the pyrene contaminated soil that contains pyrene 105mg/kg was loaded in the basin alms bowl after grinding the 3mm sieve, and balance is 4 days under 50% field capacity; To the basin alms bowl, the management method according to common crops behind plant survival manages with rye grass or red clover seedling replanting; When treating that plant grows to plant height 10～12cm, in soil, apply non-ionic surface active agent Tween80, i.e. polyoxyethylene (20) anhydrous sorbitol list olein solution, amount of application 1000mg in every kg soil; Gather soil and plant sample after 60 days, and analyze the content of pyrene in the soil, estimate repairing effect.

Cross 20 mesh sieves after the soil sample collection, take by weighing 2g in the glass centrifuge tube, add the 2g anhydrous Na ₂SO ₄, abundant mixing; After adding the 10mL carrene, ultrasonic extraction; Extract Rotary Evaporators evaporate to dryness is analyzed with high performance liquid chromatograph after the 2ml methanol constant volume.

After 60 days, the clearance of pyrene＞95% (seeing accompanying drawing) in the soil.

Embodiment 3: the pyrene contaminated soil that contains pyrene 105mg/kg was loaded in the basin alms bowl after grinding the 3mm sieve, and balance is 4 days under 50% field capacity; To the basin alms bowl, the management method according to common crops behind plant survival manages with rye grass or red clover seedling replanting; When treating that plant grows to plant height 10～12cm, in soil, apply non-ionic surface active agent Brij35, i.e. laruyl alcohol polyoxyethylene (23) ethereal solution, amount of application 100mg in every kg soil; Gather soil and plant sample after 50 days, and analyze the content of pyrene in the soil, estimate repairing effect.

Cross 20 mesh sieves after the soil sample collection, take by weighing 2g in the glass centrifuge tube, add the 2g anhydrous Na ₂SO ₄, abundant mixing; After adding the 10mL carrene, ultrasonic extraction; Extract Rotary Evaporators evaporate to dryness is analyzed with high performance liquid chromatograph after the 2ml methanol constant volume.

After 50 days, the clearance of pyrene＞96% in the soil.

Embodiment 4: the pyrene contaminated soil that contains pyrene 105mg/kg was loaded in the basin alms bowl after grinding the 3mm sieve, and balance is 4 days under 50% field capacity; To the basin alms bowl, the management method according to common crops behind plant survival manages with rye grass or red clover seedling replanting; When treating that plant grows to plant height 10～12cm, in soil, apply non-ionic surface active agent Brij35, i.e. laruyl alcohol polyoxyethylene (23) ethereal solution, amount of application 1000mg in every kg soil; Gather soil and plant sample after 60 days, and analyze the content of pyrene in the soil, estimate repairing effect.

Cross 20 mesh sieves after the soil sample collection, take by weighing 2g in the glass centrifuge tube, add the 2g anhydrous Na ₂SO ₄, abundant mixing; After adding the 10mL carrene, ultrasonic extraction; Extract Rotary Evaporators evaporate to dryness is analyzed with high performance liquid chromatograph after the 2ml methanol constant volume.

After 60 days, the clearance of pyrene＞95% in the soil.

Embodiment 5: the phenanthrene-polluted soil that contains luxuriant and rich with fragrance 68.5mg/kg was loaded in the basin alms bowl after grinding the 3mm sieve, and balance is 4 days under 50% field capacity; To the basin alms bowl, the management method according to common crops behind plant survival manages with rye grass or red clover seedling replanting; When treating that plant grows to plant height 10～12cm, in soil, apply non-ionic surface active agent Tween80, i.e. polyoxyethylene (20) anhydrous sorbitol list olein solution, amount of application 100mg in every kg soil; Gather soil and plant sample after 50 days, and analyze the content of soil China and Philippines, estimate repairing effect.

Cross 20 mesh sieves after the soil sample collection, take by weighing 2g in the glass centrifuge tube, add the 2g anhydrous Na ₂SO ₄, abundant mixing; After adding the 10mL carrene, ultrasonic extraction; Extract Rotary Evaporators evaporate to dryness is analyzed with high performance liquid chromatograph after the 2ml methanol constant volume.

After 50 days, the clearance of soil China and Philippines＞94%.

Embodiment 6: the phenanthrene-polluted soil that contains luxuriant and rich with fragrance 68.5mg/kg was loaded in the basin alms bowl after grinding the 3mm sieve, and balance is 4 days under 50% field capacity; To the basin alms bowl, the management method according to common crops behind plant survival manages with rye grass or red clover seedling replanting; When treating that plant grows to plant height 10～12cm, in soil, apply non-ionic surface active agent Tween80, i.e. polyoxyethylene (20) anhydrous sorbitol list olein solution, amount of application 1000mg in every kg soil; Gather soil and plant sample after 60 days, and analyze the content of soil China and Philippines, estimate repairing effect.

Cross 20 mesh sieves after the soil sample collection, take by weighing 2g in the glass centrifuge tube, add the 2g anhydrous Na ₂SO ₄, abundant mixing; After adding the 10mL carrene, ultrasonic extraction; Extract Rotary Evaporators evaporate to dryness is analyzed with high performance liquid chromatograph after the 2ml methanol constant volume.

After 60 days, the clearance of soil China and Philippines＞96%.

Claims (1)

1 one kinds of surfactant Synergistic plants are repaired the method for polycyclic aromatic hydrocarbon pollution, it is characterized in that the step of method is as follows:

1) plant rye grass (Lolium multiflorum Lam) or red clover (Trifolium pretense L.) plant in polycyclic aromatic hydrocarbons contaminated soil, the planting density of rye grass or red clover is every square metre 200～250 strain;

When 2) treating that plant grows to plant height 10～12cm, in soil, apply non-ionic surfactant polyoxyethylene (20) anhydrous sorbitol list olein or lauryl alcohol polyethylene glycol oxide (23) ethereal solution, the amount of application 15～150g of non-ionic surface active agent in every square metre of soil.

3) after growing 50～60 days, gather in the crops plant, dry and be transferred to other local concentrating and burn, get final product.