CN1562334A - Method of supercritical CO2 for extracting effective constituent from whole spores of ganoderma lucidum - Google Patents
Method of supercritical CO2 for extracting effective constituent from whole spores of ganoderma lucidum Download PDFInfo
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- CN1562334A CN1562334A CN 200410017468 CN200410017468A CN1562334A CN 1562334 A CN1562334 A CN 1562334A CN 200410017468 CN200410017468 CN 200410017468 CN 200410017468 A CN200410017468 A CN 200410017468A CN 1562334 A CN1562334 A CN 1562334A
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- ganoderma
- ganoderma spore
- sporophore
- spore
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 240000008397 Ganoderma lucidum Species 0.000 title 1
- 235000001637 Ganoderma lucidum Nutrition 0.000 title 1
- 239000000470 constituent Substances 0.000 title 1
- 241000222336 Ganoderma Species 0.000 claims abstract description 92
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims description 36
- 239000000284 extract Substances 0.000 claims description 25
- 239000011802 pulverized particle Substances 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 10
- 230000006837 decompression Effects 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 239000004615 ingredient Substances 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 2
- 150000003648 triterpenes Chemical class 0.000 description 8
- 150000004676 glycans Chemical class 0.000 description 7
- 229920001282 polysaccharide Polymers 0.000 description 7
- 239000005017 polysaccharide Substances 0.000 description 7
- 239000012530 fluid Substances 0.000 description 4
- 238000010298 pulverizing process Methods 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
A process for extracting the active components from ganoderma spore by supercritical CO2 includes mixing the ganoderma spora with its sporophores, adding edible alcohol, introducing supercritical CO2, and extracting. Its advantage is high extracting rate.
Description
Technical field
The present invention relates to a kind of extracting method of effective component of glossy ganoderma, particularly a kind of employing supercritical CO
2The method of effective ingredient in the extraction ganoderma spore.
Background technology
As everyone knows, Ganoderma is had the immunologic function that improves human body by the mushroom that successive dynasties medicine scholar is described as " Ganoderma ".The effective ingredient that extracts from Ganoderma as Ganoderma triterpenoids, ganoderan, can suppress the propagation of the synthetic and T of lymphocyte DNA, bone-marrow-derived lymphocyte under certain conditions.For this reason, how from Ganoderma, especially effective component extracting once from ganoderma spore becomes a research focus.Said ganoderma spore refers to Ganoderma spore and Ganoderma sporophore.Conventional at present supercritical CO
2Extracting process as the disclosed technology of Chinese patent ZL99123952.0, is effective component extracting from Ganoderma spore separately, because Ganoderma spore is at supercritical CO
2Need carry out wall breaking pulverization during extraction, the Ganoderma spore that is obtained is often reunited together, has influenced extraction efficiency, and in the effective ingredient that is extracted, polysaccharide only is 0.24%, and triterpene is 7.69%, can not satisfy the demand, and needs badly and will improve.
Summary of the invention
The technical issues that need to address of the present invention are the supercritical COs that disclose effective ingredient in a kind of ganoderma spore
2Extracting process to overcome the above-mentioned defective that prior art exists, satisfies the needs of the parties concerned.
Method of the present invention comprises the steps:
To pulverize to particulate Ganoderma sporophore and Ganoderma spore mixture place extraction kettle, feed the CO that is in supercriticality
2, the control temperature is 32 ℃～48 ℃, pressure 12Mpa～40Mpa, and the time is 3～6 hours, from the effusive CO that contains of said extraction kettle
2Fluidic ganoderma spore extract enters separating still, after the decompression cooling, makes to be dissolved in supercritical CO
2Ganoderma spore extract under the state is resolved, CO
2Flow out from separating still top, and the ganoderma spore extract that is oil, paste attitude adopts conventional method to make with extra care from the outflow of decompression separation device bottom then.
According to optimized technical scheme of the present invention, Ganoderma spore and Ganoderma sporophore preferably are crushed to 150 orders～400 purpose fine graineds.
The weight ratio of Ganoderma spore and Ganoderma sporophore is: Ganoderma sporophore: Ganoderma spore=1～25: 5.
In order further to improve extraction efficiency, the present invention also comprises the steps:
When the batch mixing of Ganoderma spore and Ganoderma sporophore pulverized particles is put into extraction kettle and is extracted, in extraction kettle, add and guide and support agent, it is said that to guide and support agent be that weight concentration is 45%～95% edible ethanol, the ganoderma spore extract that is obtained adopts conventional force method to separate with alcoholic acid mix products, obtains the ganoderma spore extract.
Ganoderma spore and Ganoderma sporophore pulverized particles: guide and support agent=1: 1～0.3, weight ratio;
According to the present invention, can be that 45%～95% edible ethanol soaks into the mixture weight concentration of the pulverized particles of Ganoderma spore and Ganoderma sporophore, and then put into extraction kettle and carry out supercritical CO
2Extraction obtains the mixed liquor of ganoderma spore extract and edible ethanol through separation, adopts conventional method separation then, obtains the ganoderma spore extract.
The time that the mixture of the pulverized particles of Ganoderma spore and Ganoderma sporophore soaks in edible ethanol is 6～24 hours;
Adopt above-mentioned method, not only can improve the dissolubility of solute in supercritical fluid, and increased the polarity of extract, thereby the important active ingredients such as ganoderan in Ganoderma sporophore and the Ganoderma spore are fully extracted, both improve extraction yield, also improved the content of active ingredient in the extract.
According to the present invention, can adopt the distillatory method of rotation under 45～55 ℃ of conditions, edible ethanol is separated from mixed liquor, obtain ganoderma spore extract (oil, cream).
Advantage of the present invention:
Compare supercritical CO of the present invention with traditional decocting in water Ganoderma sporophore, the extraction process of squeezing Ganoderma spore
2Extraction process flow is simple, saves energy consumption; Owing to extracting near under the room temperature, do not destroying the effective protoplasm composition in Ganoderma sporophore and the Ganoderma spore.Simultaneously, the present invention adopts Ganoderma sporophore and Ganoderma spore to carry out the compatibility extraction by a certain percentage, the extract of single relatively Ganoderma sporophore of extract or Ganoderma spore can more intactly embody the essential advantage of Ganoderma, can according to target require to design different component ratios, the ratio of the effective component content in the Ganodermas such as triterpenes in the extract, polysaccharide body, ucleosides and polypeptide can be regulated and control in certain limit, make the application of product have more specific aim.Simultaneously, the pulverized particles batch mixing with Ganoderma spore and Ganoderma sporophore carries out supercritical CO
2Extraction, the pulverized particles of Ganoderma sporophore becomes a kind of filler again, makes CO
2Fluid is smooth and easy in extraction process to flow the extraction efficiency height.Overcome Ganoderma spore powder and done supercritical CO
2Run into during extraction because of the spore granule is too thin, CO
2The unobstructed degree of fluid is poor, so that the low defective of extraction efficiency, extraction efficiency is improved greatly, adopt the examination criteria of edible fungi quality monitoring inspection center of the Ministry of Agriculture that extract is tested, wherein, in the effective ingredient that is extracted, polysaccharide is 3.61%, triterpene is 18.16%, far above traditional method, can satisfy the needs in relevant field.
Description of drawings
Fig. 1 is a flow chart of the present invention.
The specific embodiment
Referring to Fig. 1, method of the present invention comprises the steps:
To pulverize to particulate Ganoderma sporophore and Ganoderma spore mixture place extraction kettle 1, feed supercritical CO from compressor 5
2Fluid, the control temperature is 32 ℃～48 ℃, pressure 12Mpa～40Mpa, the time is 3～6 hours, from the said extraction kettle 1 effusive CO that contains
2Fluidic ganoderma spore extract enters separating still 2, CO by air relief valve 3
2Flow out from separating still 2 tops, get back to CO
2Groove jar 4 recycles, and flows out from separating still 2 bottoms and be ganoderma spore extract oily, the paste attitude.
And can be as required in extraction kettle 1, add and guide and support agent-edible ethanol.
Embodiment 1
With 10 kilograms of pulverizing is that the Ganoderma spore of 200 purpose Ganoderma sporophories and 20 kilograms places extraction kettle 1, feeds CO
2, the control temperature is 32 ℃, pressure 12Mpa, and the time is 4 hours, from the said extraction kettle 1 effusive CO that contains the ganoderma spore extract
2Enter separating still 2, CO
2Flow out from separating still 2 tops, and 1.6 kilograms of ganoderma spore extracts that are oil, paste attitude flow out from decompression separation device 2 bottoms.
Wherein: polysaccharide is 0.36%, and triterpene is 8.07%.
Embodiment 2
With 20 kilograms of pulverizing is that 400 purpose Ganoderma sporophories and 10 kilograms of Ganoderma spores place extraction kettle 1, feeds CO
2, the control temperature is 48 ℃, pressure 30Mpa, and the time is 5 hours, from the said extraction kettle 1 effusive CO that contains the ganoderma spore extract
2Enter decompression separation device 2, CO
2Flow out from decompression separation device 2 tops, and 1.2 kilograms of ganoderma spore extracts that are oil, paste attitude flow out from decompression separation device 2 bottoms.
Wherein: polysaccharide is 2.33%, and triterpene is 12.16%.
Adopt the method identical with embodiment 1, but when extraction, adds 3 kilogram weights of concentration and be 70% edible ethanol as guiding and supporting agent, that acquisition is is oily, 2.5 kilograms of the ganoderma spore extracts of paste attitude, and wherein: polysaccharide is 1.28%, and triterpene is 11.24%.
Adopt the method identical, be 95% edible ethanol infiltration 16 hours with the mixture weight concentration of the pulverized particles of Ganoderma spore and Ganoderma sporophore, and then put into extraction kettle and carry out supercritical CO with embodiment 2
2Extraction obtains 2.6 kilograms of ganoderma spore extracts, and wherein: polysaccharide is 3.16%, and triterpene is 18.18%.
Claims (7)
1. the supercritical CO of effective ingredient in the ganoderma spore
2Extracting process is characterized in that, comprises the steps:
To pulverize to particulate Ganoderma sporophore and Ganoderma spore mixture place extraction kettle, feed the CO that is in supercriticality
2, the control temperature is 32 ℃～48 ℃, pressure 12Mpa～40Mpa, and the time is 3～6 hours, from the effusive CO that contains of extraction kettle
2Fluidic ganoderma spore extract enters separating still, the CO after the decompression cooling
2Flow out from separating still top, flow out from decompression separation device bottom and be ganoderma spore extract oily, the paste attitude.
2. method according to claim 1 is characterized in that, Ganoderma spore and Ganoderma sporophore are crushed to 150 orders～400 purpose fine graineds.
3. method according to claim 1 is characterized in that, the weight ratio of Ganoderma spore and Ganoderma sporophore is: Ganoderma sporophore: Ganoderma spore=1～25: 5.
4. according to claim 1,2 or 3 described methods, it is characterized in that the present invention also comprises the steps:
When the batch mixing of Ganoderma spore and Ganoderma sporophore pulverized particles is put into extraction kettle and extracted, add in extraction kettle and guide and support agent, said to guide and support agent be that weight concentration is 45%～95% edible ethanol.
5. method according to claim 4 is characterized in that, Ganoderma spore and Ganoderma sporophore pulverized particles: guide and support agent=1: 1～0.3, weight ratio.
6. according to claim 1,2 or 3 described methods, it is characterized in that, be that 45%～95% edible ethanol soaks into the mixture weight concentration of the pulverized particles of Ganoderma spore and Ganoderma sporophore, and then put into extraction kettle and carry out the supercritical CO 2 extraction.
7. method according to claim 6 is characterized in that, the time that the mixture of the pulverized particles of Ganoderma spore and Ganoderma sporophore soaks in edible ethanol is 6～24 hours.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410017468 CN1273147C (en) | 2004-04-05 | 2004-04-05 | Method of supercritical CO2 for extracting effective constituent from whole spores of ganoderma lucidum |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410017468 CN1273147C (en) | 2004-04-05 | 2004-04-05 | Method of supercritical CO2 for extracting effective constituent from whole spores of ganoderma lucidum |
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| CN1562334A true CN1562334A (en) | 2005-01-12 |
| CN1273147C CN1273147C (en) | 2006-09-06 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831351A (en) * | 2010-06-03 | 2010-09-15 | 吉林大学 | Method for extracting ganoderma oil with supercritical carbon dioxide fluid |
| CN101120955B (en) * | 2006-08-11 | 2012-09-26 | 北京协和药厂 | Enteric coated preparation containing glossy ganoderma spore powder active ingredient |
| CN103351941A (en) * | 2013-06-17 | 2013-10-16 | 浙江龙泉佳宝生物科技有限公司 | Supercritical CO2 variable-temperature variable-pressure extraction method of lucid ganoderma spore oil |
| CN105968257A (en) * | 2016-04-15 | 2016-09-28 | 南京中医药大学 | Preparation method and application of ganoderic acid A molecular imprinted polymer |
| CN113150867A (en) * | 2020-12-01 | 2021-07-23 | 中科健康产业集团股份有限公司 | Preparation method of ganoderma lucidum extract oil rich in ganoderma lucidum triterpenes |
-
2004
- 2004-04-05 CN CN 200410017468 patent/CN1273147C/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101120955B (en) * | 2006-08-11 | 2012-09-26 | 北京协和药厂 | Enteric coated preparation containing glossy ganoderma spore powder active ingredient |
| CN101831351A (en) * | 2010-06-03 | 2010-09-15 | 吉林大学 | Method for extracting ganoderma oil with supercritical carbon dioxide fluid |
| CN103351941A (en) * | 2013-06-17 | 2013-10-16 | 浙江龙泉佳宝生物科技有限公司 | Supercritical CO2 variable-temperature variable-pressure extraction method of lucid ganoderma spore oil |
| CN103351941B (en) * | 2013-06-17 | 2015-06-17 | 浙江龙泉佳宝生物科技有限公司 | Supercritical CO2 variable-temperature variable-pressure extraction method of lucid ganoderma spore oil |
| CN105968257A (en) * | 2016-04-15 | 2016-09-28 | 南京中医药大学 | Preparation method and application of ganoderic acid A molecular imprinted polymer |
| CN105968257B (en) * | 2016-04-15 | 2018-09-25 | 南京中医药大学 | A kind of preparation method and applications of ganoderic acid A molecularly imprinted polymers |
| CN113150867A (en) * | 2020-12-01 | 2021-07-23 | 中科健康产业集团股份有限公司 | Preparation method of ganoderma lucidum extract oil rich in ganoderma lucidum triterpenes |
| WO2022116807A1 (en) * | 2020-12-01 | 2022-06-09 | 中科健康产业集团股份有限公司 | Method for preparing ganoderma lucidum extract oil rich in ganoderma lucidum triterpene |
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| CN1273147C (en) | 2006-09-06 |
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