CN1560601A - Portable investigating instrument for food and environment pollutant and its application - Google Patents
Portable investigating instrument for food and environment pollutant and its application Download PDFInfo
- Publication number
- CN1560601A CN1560601A CNA2004100107062A CN200410010706A CN1560601A CN 1560601 A CN1560601 A CN 1560601A CN A2004100107062 A CNA2004100107062 A CN A2004100107062A CN 200410010706 A CN200410010706 A CN 200410010706A CN 1560601 A CN1560601 A CN 1560601A
- Authority
- CN
- China
- Prior art keywords
- environmental contaminants
- pack
- measure
- developer
- detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention belongs to detecting instrument and the application in food and environment contamination material detection and analysis. The structure is made up of light source, absorbing pool, detector, amplifying and reading device, displaying screen, the light source is a high bright illuminant diode; the absorbing pool is cork cylinder absorbing pool. The structure also includes agent package, is filled with measuring agent. The invention constructs the special agent and technology applied to the detection instrument in the invention, it is more proper to the spot detection to formaldehyde, pesticide residual poison, dissolved oxygen. The invention has a high sensitivity, precision and detection limit better than current spectrophotometer; the volume is small, the weight is light, and it uses special agent, it can carry conveniently and carries on spot analysis. The agent is stable, easy to be reserved; the reaction condition is mild, the reaction is quick.
Description
Technical field
The invention belongs to a kind of detecting instrument, particularly can carry out the portable instrumentation of on-the site analysis food and environmental contaminants, and at formaldehyde, residual toxicity, the application in the analysis technology such as oxygen in water.
Background technology
Along with science and technology development, analytical chemistry shows some new characteristics, and this shows: 1, from the angle of analytical instrument, important developing direction of analytical instrument is to miniaturization, microminiaturized development, progressively walk out the laboratory, become ordinary people's instrument.2, from the angle of analytical approach, development of new reagent is some special-purpose test agent and improve available reagent to make it have better effect be important research contents in the analytical approach field especially.
On the method that grain, food and environmental contaminants detect, generally adopt spectrophotometric method now, spectrophotometric method is the measuring method of a kind of routine in the analytical chemistry, has obtained using widely because of it has easy and simple to handle, characteristics such as usable range is wide, sensitivity height.In China present stage, in fields such as environment, medicine, materials, spectrophotometric method all has important use under study for action, without exception outfit spectrophotometer almost in various levels of assay laboratory.
With the close background technology of the present invention is common spectrophotometer, and its structure mainly includes power supply, light source, beam splitting system, do not have the prism-shaped absorption cell of plug, detecting device, control chip, a display screen etc.The present widely used spectrophotometer of China is through the development of 721 to 751 types of associating, though obtained on the function improving and improved, but still come with some shortcomings, this shows: light source adopts tungsten lamp, and the intensity of light source is not very stable, makes that its precision is lower; Adopt the beam splitting system of mechanical scanning, this system price is higher but fragile structure and stability are not good; Used detectable instability, some reagent are toxic, therefore are difficult for preserving, carrying, and use inconvenient; Price of complete machine is higher, and volume is bigger, can not be used for on-the site analysis, can't walk out the laboratory.
Summary of the invention
Spectrophotometer is walked out the laboratory becomes portable instrument, must be accompanied by the detectable especially exploitation and the improvement of special agent, and this reagent must have such characteristics: stable, preserve easily; The reaction conditions gentleness, the speed that reacts completely is fast; Simple to operate, easy to carry.Develop this class reagent and walk out the laboratory, become portable instrument and play a crucial role for food and environmental contaminants detection type instrument.
Shortcoming and defect at background technology the invention provides a kind of high sensitivity, precision is good, detection limit is low, compact conformation, volume are little and in light weight, a kind of portable food and the environmental contaminants detector of applied range; And set up several corresponding special agent and detection techniques that are applied to portable food and environmental contaminants detector, make portable food and environmental contaminants detector be more suitable for carrying out on-site measurement.
Portable food of the present invention and environmental contaminants detector, constitute by power supply, light source, absorption cell, detecting device, control chip, display screen, absorption cell places between light source and the detecting device, detecting device is connected with display screen by amplification and reading device, it is characterized in that said light source is highlighted light emitting diode; Said absorption cell is the cylindrical absorbent pond that has gag.Saved the beam splitting system part of background technology.
Light source adopts highlighted light emitting diode (LED), and different wave length LED had both made photometric light source, makes monochromator again, according to the light absorbing wavelength difference of measured matter, can make things convenient for the light emitting diode of sampling different wave length.Therefore saved the beam splitting system in the background technology, and highlighted light emitting diode long service life not only, low price, and utilize the pulse power supply mode, power consumption is also very low.
Tool plug cylindrical absorbent pond can prevent that solution from overflowing, and makes the reaction tube of sample and reagent reacting again, need not to shift sample, realizes continuous detecting.
Said detecting device can be used phototriode, also can be with photoelectric tube or photomultiplier.
Measurement result shows on display screen, easily reading
Above-mentioned portable food and environmental contaminants detector can be made portable detector, are used for on-the site analysis easily.Weight is no more than 6kg, and volume is no more than 1L.
Effect of the present invention mainly contains: 1, highly sensitive, and be that example detects and is limited to 5ppb to survey phosphorus; With traditional spectrophotometric instrumentation phosphorus low 3 orders of magnitude; Precision and detection limit all are better than existing spectrophotometer.2, volume is little, in light weight, and complete machine weight is no more than 6 kilograms, is beneficial to and carries, and can easily carry out on-the site analysis.3, processing is simple and easy, and cost is low, easily industrialization.4, usable range is wide, removes the mensuration that the inorganic constituents can measure in the normal food environmental contaminants and organic principle can also be used for some complicated pollutants.
The structure of portable food of the present invention and environmental contaminants detector also includes pack, loading testeding agent in it.
In order to make food and environmental contaminants detector become portable instrument, its analysis must have stable with chemical reagent, preserve easily; The reaction conditions gentleness, the speed that reacts completely is fast; Simple to operate, characteristics such as easy to carry only possess these characteristics and just can make portable food and environmental contaminants detector be used for on-the-spot on-line analysis.Designed and be used for formaldehyde, residual toxicity, the special agent bag of DO determination for reaching above purpose the present invention.
The formaldehyde pack
In the current formaldehyde detection method of China, three kinds of detection methods are recommended by State General Administration for Quality Supervision, and method one is the Lead acetate paper method, and method two is the chromatography of ions, and method three is a photometry.Wherein, method one only is a quilitative method, and poor sensitivity can not adapt to the needs of detection; Method two and method three all need the way of distillation to carry out sample pre-treatments, and operation steps is numerous and diverse, should not control, and experimental period is long, and causes sensitivity lower.Therefore, said method in order to carry out formaldehyde in food measure be difficult to accomplish promptly fast, accurate again, also be difficult to realize the purpose of on-the site analysis.
For addressing the above problem the method that the invention provides a kind of formaldehyde special agent bag, utilize the formaldehyde in the water extraction extraction sample, utilize lead acetate and potassium oxalate-sodium hydrogen phosphate as protein precipitant with the albumen precipitation in the water extract, measure with developer reaction back.
According to above-mentioned measuring method, lead acetate and potassium oxalate-sodium hydrogen phosphate are housed as protein precipitant in the pack, diacetone is housed as developer.Precipitation agent and developer pack respectively, help long preservation, and are easy to carry.
According to above-mentioned measuring method, it is characterized in that pre-treating method uses water extraction, can avoid the trivial operations of the way of distillation, uppity shortcoming like this, help like this realizing that instrument carries out on-the site analysis.
According to above-mentioned measuring method, it is characterized in that having adopted lead acetate and potassium oxalate-sodium hydrogen phosphate as protein precipitant, in the time of can having avoided in GB with lead acetate and potassium ferrocyanide as precipitation agent like this, it is unfavorable that solution is the chromogenic reaction of acid PARA FORMALDEHYDE PRILLS(91,95) and diacetone, and potassium ferrocyanide solution can produce shortcomings such as light yellow after placing.
The residual toxicity pack
Gas chromatography, look-instrument analytical methods such as matter coupling, the requirement of incompatibility field quick detection are adopted in the detection of organic phosphates and carbamate pesticide residue usually in the vegetables.About rapid analysis, what foreign literature was more is the electrochemical enzymatic sensor method, also has the report of microwell plate microplate reader method.There is the electrode vulnerable to pollution in the former and needs the trouble of Regeneration Treatment; The latter is difficult to realize on-the-spot the detection because of being subjected to the restriction of experiment condition.
For addressing the above problem, the present invention adopts Dynamics of Enzyme Catalysis spectrphotometric method for measuring residual toxicity, is catalyzer with acetylcholinesterase, utilizes the chromogenic reaction of acetylthiocholine iodide and developer, measures absorbance and measures residual toxicity content.
According to above-mentioned measuring method, acetylcholinesterase is housed as catalyzer in the pack, acetylthiocholine iodide is housed as substrate, 5,5 '-two sulfo-s-2 are housed, 2 '-dinitrobenzoic acid is as developer.And developer, catalyzer and substrate packed respectively.
According to above-mentioned measuring method, the catalyzer acetylcholinesterase that is adopted can be that the enzyme of handling like this can long preservation through stabilization processes.The method that the stabilization processes of said acetylcholinesterase adopts can be to make enzyme remain stable by add protectant method in liquid enzymes; Also can be to adopt the solid enzyme, make enzyme combine the purpose that reaches stable with carrier by enzyme immobilization technology.
The result of use of residual toxicity pack: 1, reaction velocity is fast; 2, reappearance, detection limit and traditional spectrophotometric method detect and more all have superiority.3, good stability, normal temperature can keep 15 days, can preserve in refrigerator 1 year, and the enzyme reagent holding time is improved greatly, can satisfy the on-the-spot requirement that detects.
The DO determination pack
The common method of measuring dissolved oxygen DO has iodimetric titration and revised law thereof, electrochemical method.In these common methods, all because instrument complexity, the method used are loaded down with trivial details, the serious interference of air, organism etc. etc. is former be difficult to analyze at the scene in use, the reagent of the spectrphotometric method for measuring dissolved oxygen DO of been reported has dyestuffs such as methylene blue and indigo carmine.Indigo carmine is subjected to extensive concern because of it is highly sensitive, but the cyanogen that must use severe toxicity when the indigo carmine of ortho states is gone back in preparation makes its application be restricted for sodium borohydride.
For overcoming the above problems, the present invention such as cellulose, has made a kind of liquid reagent of DO determination special use going back one or more helper components of interpolation on the indigo carmine basis of ortho states.This reagent is encapsulated in special glass tube---in the color comparison tube, adopt the method for visual colorimetric determination and portable food and the quantitative measurment of environmental contaminants detector to measure dissolved oxygen content in the water respectively.
According to above-mentioned measuring method, indigo carmine and the liquid reagent bottle of helper component, the i.e. color comparison tube of going back ortho states are housed in the pack.
According to above-mentioned measuring method, the present invention can also be with the nontoxic reagent preparation indigo carmine of ortho states also, and this has been avoided using cyanogen of severe toxicity for sodium borohydride.
According to above-mentioned measuring method, it is characterized in that adding auxiliary element going back on the indigo carmine basis of ortho states, make a kind of DO determination special agent, can eliminate common oxygenant, as Cu
2+, Fe
3+, V
5+, Cl
2Deng interference.
According to above-mentioned measuring method, it is characterized in that a kind of color comparison tube and sampling jar special, concrete structure is seen embodiment 2.Can take a sample accurately and conveniently with this color comparison tube and sampling jar, the method of employing visual colorimetric determination and portable food and the quantitative measurment of environmental contaminants detector is measured the dissolved oxygen content in the water, this sampling method has been avoided the interference that air causes in the routine measurement method sampling process, and convenient and swift.
The characteristics of DO determination pack: 1, can be convenient and swift and the dissolved oxygen concentration in the on-site measurement natural water economically; 2, adopt the pollution of avirulence reagent minimizing, also reduced harm testing crew to environment.3, avoided the interference that air causes in the routine measurement method sampling process, also eliminated the interference of common oxygenant, made measurement more accurate.4, reagent carries out sealed package, is easy to carry about with one, and is suitable for field condition and measures.
Description of drawings
Fig. 1 is an internal optics component locations structural drawing of the present invention;
Fig. 2 is a schematic appearance of the present invention;
Fig. 3 is color comparison tube and the sampling jar structural representation that DO determination of the present invention is used.
Embodiment
Below in conjunction with accompanying drawing, the concrete structure of the present invention and the course of work are described.
Embodiment 1
This portable food and environmental contaminants detector are traditional spectrophotometric improvement and innovation aspect instrument, but are different from traditional spectrophotometer.As shown in Figure 1: after the light that light source 1 sends focuses on via lens 2, focus on the side of tool plug column type absorption cell 4, the upper bottom surface opening of tool plug column type absorption cell 4 also seals with gag, after the reagent effect in light and the absorption cell 4, penetrate by absorption cell 4 opposite flanks, shine on the detecting device 5 via lens 3 focusing, after amplification and reading device 6 (comprising amplifying circuit and reading circuit) processing, on display screen, show measurement result.Above-mentioned light source 1 can be light emitting diode, high intensity laser beam device or integrated light-emitting diode (LED) or LED display; Described absorption cell 4 is cylindric absorption cells, and its material can be glass or quartz, and described detecting device 5 can be phototriode or photoelectric tube, photomultiplier; Multiplying arrangement 6 can adopt logarithm to amplify or two kinds of amplification systems of common amplification respectively, can carry out measurement pattern and select.
The electric component of instrument has mainly been concentrated on instrument top, comprises power supply, control chip, detecting device and switch etc.And optics concentrates on the instrument bottom, the putting in order as Fig. 1 of optics.
Fig. 2 is portable food of the present invention and environmental contaminants detector schematic appearance.Use for convenient, at instrument in appearance, be provided with display screen clearly to show measurement result; Be provided with the electricity consumption of power switch with control light source and detecting device, promptly control instrument using and closing; Be provided with the zero-setting button that links to each other with amplifying circuit in order to zeroing; Be provided with mode selection switch to carry out the selection of measurement pattern; For the convenient open-air power supply that uses also can be battery.
Embodiment 2
Fig. 3 is color comparison tube and the sampling jar structural representation that DO determination of the present invention is used.Among Fig. 3,7 is color comparison tube, is the container of the dropper shape of a upper end tool plug, and the termination of lower end tubule is sealed, and is used with sampling jar 8; Sampling jar 8 is inverted round table shapes, its bottom surface tipping water inlet pipe 11, and there is annular recess 10 the bottom surface inboard.The bottom that the width of groove 10 can make the termination of color comparison tube 7 lower end tubules insert groove 10 is broken color comparison tube 7 tops gently off with the fingers and thumb color comparison tube 7 lower end tubules are fractureed during use.
Embodiment 3
Among the present invention about utilizing portable food and environmental contaminants detector to measure an example of formaldehyde:
Preparation developer diacetone solution: in 100mL distilled water, add ammonium acetate 25g, glacial acetic acid 3mL and diacetone 0.40mL, jolting is short molten, lays in brown bottle, and the pack of packing into is stand-by.This liquid can be preserved 1 month.
Sample is smashed to pieces with tissue mashing machine, takes by weighing the water sample 10g that delivers vegetables, and dry vegetalbe sample 5g (being accurate to 0.01g) is in the capacity measuring bottle of 100mL, add entry to 60mL, cover tight lid, place (60 ± 5) min, every 5min shakes bottle once, adds 10.0mL lead acetate solution, shakes up gently, add 10.0mL potassium oxalate-disodium phosphate soln again, behind the jog, water is settled to scale, shakes up, leave standstill, use the middling speed filter paper filtering.
Pack into absorption cell 4 usefulness portable foods and environmental contaminants detector of filtrate detects.
Embodiment 4
Among the present invention about utilizing portable food and environmental contaminants detector to measure an example of residual toxicity:
(vegetable sample that is accurate to ± 0.1g) shreds is put into tool plug test tube, adds 10mL buffer solution, and 2min is extracted in vibration, places 3-5min, makes upper strata liquid clarification to weigh 1g; Survey the blank sample earlier, take out 4.0mL clear liquid (in the blank sample, adding 4.0mL buffer solution) in absorption cell 4, add 50 μ L acetylcholinesterase liquid and acetylthiocholine iodide liquid respectively, hand is sealed insulation 5.0min, and absorption cell 4 is inserted light path, zeroing, take out the back and add 40 μ L acetylcholinesterase liquid, insert light path behind the shake 20-30s immediately, by measuring key, the program opening entry stops behind the 3min; Repeat above step, carry out sample successively continuously and measure, circulation of every 3min, instrument provides the sample enzyme inhibition rate automatically, and judgement sample is subjected to the pollution level of agricultural chemicals in view of the above.
Embodiment 5
Among the present invention about utilizing portable food and environmental contaminants detector to measure an example of dissolved oxygen DO:
Indigo carmine and the cellulosic color comparison tube 7 of helper component of going back ortho states will be housed earlier to be placed in the hand-held sampling jar 8, make the bottom of the termination insertion groove 10 of color comparison tube 7 lower end tubules, then with the color comparison tube 7 apicule ends that fracture in the groove 10 of color comparison tube 7 tips in the sampling jar 8 that water sample is housed, water sample can enter in the color comparison tube 7 under atmospheric effect, makes reagent change indigo plant in the pipe.Press the on ﹠ off switch energized, by the zeroing key, make to show 0.00 on the LCDs again, replace absorption cell 4 to be placed in portable food and the environmental contaminants detector color comparison tube 7, by the concentration that can read dissolved oxygen DO this moment behind the read key.
Claims (7)
1, a kind of portable food and environmental contaminants detector, constitute by power supply, light source (1), absorption cell (4), detecting device (5), display screen, absorption cell (4) places between light source (1) and the detecting device (5), detecting device (5) is connected with display screen by amplification and reading device (6), it is characterized in that said light source (1) is highlighted light emitting diode; Said absorption cell (4) is the cylindrical absorbent pond that has gag.
2, according to described portable food of claim 1 and environmental contaminants detector, it is characterized in that structure includes pack, loading testeding agent in it.
3, a kind of portable food of claim 1 and the application of environmental contaminants detector in formaldehyde, residual toxicity, DO determination, it is characterized in that, with lead acetate and potassium oxalate-sodium hydrogen phosphate as protein precipitant, diacetone is as developer, protein precipitant and developer pack respectively and are placed in the pack, are used to measure formaldehyde; Or with acetylcholinesterase as catalyzer, acetylthiocholine iodide is as substrate, 5,5 '-two sulfo-s-2,2 '-dinitrobenzoic acid is as developer, and developer, catalyzer and substrate pack respectively and be placed in the pack, are used to measure residual toxicity; Maybe will adorn indigo carmine and the cellulose liquid reagent bottle of going back ortho states and be placed in the pack, be used to measure dissolved oxygen DO.
4, the application in formaldehyde determination according to described portable food of claim 3 and environmental contaminants detector, it is characterized in that, utilize the formaldehyde in the water extraction extraction sample, utilize lead acetate and potassium oxalate-sodium hydrogen phosphate as protein precipitant with the albumen precipitation in the water extract, measure with developer reaction back.
5, the application in residual toxicity is measured according to described portable food of claim 3 and environmental contaminants detector, it is characterized in that, adopt Dynamics of Enzyme Catalysis spectrphotometric method for measuring residual toxicity, with acetylcholinesterase is catalyzer, utilize the chromogenic reaction of acetylthiocholine iodide and developer, measure absorbance and measure residual toxicity content.
6, the application in DO determination according to described portable food of claim 3 and environmental contaminants detector is characterized in that, indigo carmine and the cellulose liquid reagent of going back ortho states is encapsulated in the color comparison tube (7) color comparison tube (7) double as absorption cell; Adopt the method for visual colorimetric determination and quantitative measurment to measure dissolved oxygen content in the water respectively.
7, according to claim 3 or 6 described portable foods and the application of environmental contaminants detector in DO determination, it is characterized in that said color comparison tube (7) is the container of the dropper shape of a upper end tool plug, the termination of lower end tubule is sealed, and is used with sampling jar (8); Sampling jar (8) is the inverted round table shape, its bottom surface tipping water inlet pipe (11), and there is annular recess (10) the bottom surface inboard, the bottom that the width of groove (10) can make the termination of color comparison tube (7) lower end tubule insert groove (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100107062A CN1560601A (en) | 2004-02-25 | 2004-02-25 | Portable investigating instrument for food and environment pollutant and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100107062A CN1560601A (en) | 2004-02-25 | 2004-02-25 | Portable investigating instrument for food and environment pollutant and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1560601A true CN1560601A (en) | 2005-01-05 |
Family
ID=34440004
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004100107062A Pending CN1560601A (en) | 2004-02-25 | 2004-02-25 | Portable investigating instrument for food and environment pollutant and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1560601A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102116744A (en) * | 2010-02-08 | 2011-07-06 | 力德力诺生物技术(北京)有限公司 | Device for detecting hydrogen peroxide and preparation method thereof |
| CN102156105A (en) * | 2011-03-31 | 2011-08-17 | 东华理工大学 | Site uranium fast analysis method and special reagent packet for method |
| CN102384895A (en) * | 2011-11-15 | 2012-03-21 | 中华人民共和国北京出入境检验检疫局 | Field atmosphere detection system and detection method thereof |
| CN102890063A (en) * | 2012-10-10 | 2013-01-23 | 长春理工大学 | Multi-parameter portable automatic food safety detector |
| CN103837529A (en) * | 2014-03-06 | 2014-06-04 | 北京工业大学 | Detection tube for detecting content of hydrogen in water and application of detection tube |
| CN104502293A (en) * | 2014-12-30 | 2015-04-08 | 中山大学 | Quick food security detection device based on cell phone platform |
| CN106769905A (en) * | 2016-11-23 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of Rapid food detection instrument |
| CN109709040A (en) * | 2018-12-27 | 2019-05-03 | 天津昌和生物医药技术有限公司 | Miniature biochemical analysis instrument is used in a kind of detection of papery miniflow test card |
-
2004
- 2004-02-25 CN CNA2004100107062A patent/CN1560601A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102116744A (en) * | 2010-02-08 | 2011-07-06 | 力德力诺生物技术(北京)有限公司 | Device for detecting hydrogen peroxide and preparation method thereof |
| CN102156105A (en) * | 2011-03-31 | 2011-08-17 | 东华理工大学 | Site uranium fast analysis method and special reagent packet for method |
| CN102156105B (en) * | 2011-03-31 | 2012-08-08 | 东华理工大学 | Site uranium fast analysis method and special reagent packet for method |
| CN102384895A (en) * | 2011-11-15 | 2012-03-21 | 中华人民共和国北京出入境检验检疫局 | Field atmosphere detection system and detection method thereof |
| CN102890063A (en) * | 2012-10-10 | 2013-01-23 | 长春理工大学 | Multi-parameter portable automatic food safety detector |
| CN103837529A (en) * | 2014-03-06 | 2014-06-04 | 北京工业大学 | Detection tube for detecting content of hydrogen in water and application of detection tube |
| CN104502293A (en) * | 2014-12-30 | 2015-04-08 | 中山大学 | Quick food security detection device based on cell phone platform |
| CN106769905A (en) * | 2016-11-23 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of Rapid food detection instrument |
| CN109709040A (en) * | 2018-12-27 | 2019-05-03 | 天津昌和生物医药技术有限公司 | Miniature biochemical analysis instrument is used in a kind of detection of papery miniflow test card |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gerchakov et al. | Improved technique for analysis of carbohydrates in sediments 1 | |
| US8148167B2 (en) | Fluorescent pH detector system and related methods | |
| Mehrvar et al. | Fiber-optic biosensors-trends and advances | |
| Cammann et al. | Chemical sensors and biosensors—principles and applications | |
| Kostov et al. | Low-cost optical instrumentation for biomedical measurements | |
| CA2256629A1 (en) | Masking background fluorescence and luminescence in optical analysis of biomedical assays | |
| Luong et al. | Developments and applications of biosensors | |
| SE8500437D0 (en) | CHEMICAL ANALYSIS BASED ON RELATIONSHIP BETWEEN RADIATION AND A FIXED SURFACE IN A FLOOD CELL | |
| KR920701475A (en) | Measurement of glucose in whole blood and cuvettes and photometers used to perform this method | |
| CN1560601A (en) | Portable investigating instrument for food and environment pollutant and its application | |
| Schaffar et al. | Chemically mediated fiberoptic biosensors | |
| Arnold | Fluorophore-and chromophore-based fiberoptic biosensors | |
| Sanz-Medel | Solid surface photoluminescence and flow analysis: a happy marriage | |
| WO1992012424A1 (en) | An optical probe and method for monitoring an analyte concentration | |
| Avila et al. | Enzymic determination of peroxides in non-aqueous media | |
| CN101738372B (en) | Optical detection device of liquid porphyrin array and method thereof for detecting amino acid | |
| CN101750402A (en) | Optical oxygen sensor | |
| Abdel-Latif et al. | Fiber optic sensors: recent developments | |
| CN2687662Y (en) | Portable pollutant detecting instrument | |
| Narayanaswamy et al. | Optical technology until the year 2000: an historical overview | |
| Ballesta-Claver et al. | One-shot lactate chemiluminescent biosensor | |
| Chen et al. | Stopped-flow fluorometry with available instrumentation | |
| Paerl | Primary productivity and producers | |
| Lampman et al. | A method for the measurement of particulate C and P on the same filtered sample | |
| JPH02120665A (en) | Analyzer/reader |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |