CN1557942A - A insecticide and its application - Google Patents
A insecticide and its application Download PDFInfo
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- CN1557942A CN1557942A CNA031400922A CN03140092A CN1557942A CN 1557942 A CN1557942 A CN 1557942A CN A031400922 A CNA031400922 A CN A031400922A CN 03140092 A CN03140092 A CN 03140092A CN 1557942 A CN1557942 A CN 1557942A
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- rhzomorph
- sterilant
- tianshan mountains
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Abstract
The present invention is Tianshan bacterin as one kind of pesticide and its application. Tianshan bacterin is suitable for preventing and killing mite, tick and other pests and has excellent preventing and killing effect on Coleoptera pests, weevil, Dipters pest, Anoplura pest, Isoptera pest, hymenopteran ant, mite and animal parasite. The present invention has simple preparation process, less consumption, less resistance, high safety and no environmental pollution.
Description
Technical field
The present invention relates to plant protection and technical field of pesticide, specifically be meant a kind of sterilant Tianshan Mountains rhzomorph and application thereof.
Background technology
Antiinsect antibiotic is one of important biological pesticide, it is a kind of natural biological sterilant, has characteristics such as low to the person poultry toxicity, that degraded is rapid, does not have " three cause " effect, meet the requirement of crop pests such as non-environmental pollution control vegetables, thereby become one of focus of researching and developing in recent years both at home and abroad.General desinsection chemical agent makes insect that medicament is developed immunity to drugs easily, and is difficult to avoid the pollution to environment, lacks the prevention effect at resistance small cabbage moth and water spider simultaneously.
Summary of the invention
Purpose of the present invention is exactly in order to solve above-mentioned the deficiencies in the prior art part, and a kind of sterilant Tianshan Mountains rhzomorph and application thereof are provided.This sterilant Tianshan Mountains rhzomorph is produced by a new streptomyces bacterial classification, and it is applicable to control insect etc., the particularly insect of lepidopteran (Lepidoptera) and Diptera (Diptera), and it is free from environmental pollution, has attracting insecticidal effect.
The present invention at first provides the bacterial strain of a new streptomyces bacterial classification, described bacterial strain is named as Streptomyces sp.GAAS7310, it is separating obtained from pedotheque, described bacterial strain is being positioned at Chinese typical culture collection center (CCTCC) preservation in Wuhan on July 7th, 2003, and deposit number is CCTCC NO:M 203058.
A kind of sterilant of the present invention Tianshan Mountains rhzomorph is a new compound, it is characterized in that it has following molecular formula:
The application of a kind of sterilant of the present invention Tianshan Mountains rhzomorph, it is characterized in that, it is applicable to control mite, tick and other insect, comprises beet armyworm, tobacco budworm, bollworm, small white, Pyrausta nubilalis (Hubern)., small cabbage moth, leaf roller, cutworm, subtropics mythimna separata, rain moth section, India paddy phycitid, Pectinophora gossypiella; Also Coleoptera and weevil are produced effect, comprise colorado potato bug, three chrysomelid, Japanese fox rutelians of cucumber 11 asterophyllite first, cucumber, boll weevil; Also effective to Diptera pest, comprise housefly, Dulicidae, fruit bat, horn fly and Agromyzidae effective; Effective to Anoplura, Isoptera and Hymenoptera ant and mite, comprise citrus red mite, tetranychid; Simultaneously zooparasite there is fine prevention effect, comprises water flea, Chinese anchor head lice.
In order to realize the present invention better, described sterilant Tianshan Mountains rhzomorph is mixed with cream preparation when using, and its weight percent proportioning is: sterilant Tianshan Mountains rhzomorph 1～30%, emulsifying agent 5～20%, solvent 50～94%; Its best proportioning is: sterilant Tianshan Mountains rhzomorph 5～10%, emulsifying agent 8～15%, solvent 75～87%, process for preparation is: sterilant Tianshan Mountains rhzomorph and synergistic agent and selected tensio-active agent are placed reactor, it at 35 ℃ of temperature, rotating speed reaction 35 minutes under 320 rev/mins the condition, and then adding organic solvent, continue to stir 35 minutes, get final product after stirring.Wherein, emulsifying agent comprises farming breast 2201, agricultural newborn 0203B, YZ960; Solvent comprises Virahol, acetone, dimethyl formamide.
Sterilant of the present invention Tianshan Mountains rhzomorph can make by following method:
The first step inoculates GAAS7310 into the nutrition substratum, and growth is 48 hours under the vibration of 28 ℃ and 250rpm; Get sophisticated fs nutrition culture inoculating nutrient medium, obtain secondary nutrition culture, under 28 ℃ and 300rpm condition, cultivated 48 hours, sophisticated strain fermentation is cultivated, under 28 ℃ and 300rpm, keep cultivating 4 days.
The fermentation of second step finishes, mycelia is obtained in method separation with filter press, the acetone soln of mycelia with 1: 2 leached 10 hours, with the concentrated acetone that goes of leaching liquid, use the ethyl acetate extraction working substance again, the acetic acid ethyl acetate extract concentrating under reduced pressure is obtained oily matter, the extraction concentrated solution is mixed silica gel and is crossed post, with ethyl acetate, sherwood oil silica gel column chromatography and concentrate repeatedly, obtain the pure product of oily mater.
The culture feature of bacterial strain GAAS7310 of the present invention is as shown in table 1.
The morphological specificity of bacterial strain GAAS7310 of the present invention is: it produces bacterial strain is the golden monoid of streptomycete.
The physiological characteristic of bacterial strain GAAS7310 of the present invention is: gelatin does not liquefy, and milk peptonizes, hydrolyzed starch, and faint growth on the Mierocrystalline cellulose does not produce H2S, and the cell walls chemical ingredients is the I type, and full cell sugar belongs to the C type.Can utilize: sorbose, seminose, synanthrin, do not utilize: maltose, fructose, lactose, semi-lactosi, rhamnosyl, inositol.
The culture feature of table 1 GAAS7310
| Substratum | The base silk | The gas silk | Pigment |
| No. 1, Gao Shi | ??IV(6-6’) | Dark cigarette ash | ??III(6-6’) |
| Czapek's solution | Colourless or whitewash | ??III(7-1’) | |
| Glucose Tianmen asparagine | ??III(1-1’) | ??IV(6-1’) | ??III(2-2’) |
| Inorganic salt starch | Litchi meat is white | Do not have | |
| Calcium malate | ??II(2-1’) | Do not have | Nude |
| Oatmeal | The hawksbill turtle Huang | ??III(6-1’) | ??II(2-3’) |
| Glucose yeast cream | ??III(3-4’) | ??III(6-1’) | The hawksbill turtle Huang |
| Potato ball | The deer horn palm fibre | Resemble ash | ??III(4-5’) |
The present invention compared with prior art has following advantage and beneficial effect:
1. microbiotic of the present invention is a novel compound, simple, easy to operate, the easy preparation of its preparation process thereof.
2. sterilant of the present invention Tianshan Mountains rhzomorph is good to pest controling effects such as small cabbage moths, lacks than the conventional dose consumption, can overcome the resistance of insects such as small cabbage moth to conventional pesticide.
3. sterilant of the present invention Tianshan Mountains rhzomorph is safe in utilization, free from environmental pollution, is a kind of non-harmful biological pesticide.
Description of drawings
Fig. 1 is the shape synoptic diagram of bacterial strain GAAS7310 gonimoblast of the present invention and spore.
Bacterial strain Streptomyces sp.GAAS7310 of the present invention is being positioned at Chinese typical culture collection center (CCTCC) preservation in Wuhan on July 7th, 2003, and deposit number is CCTCC NO:M 203058.
Embodiment
Below in conjunction with drawings and Examples, the present invention is done detailed description further.
Embodiment one
Prepare sterilant Tianshan Mountains rhzomorph by in fermention medium, cultivating bacterial strain GAAS7310.The refrigerated GAAS7310 nutritional medium of 1ml is melted, and the 100ml nutritional medium in the 500ml triangular flask is gone in inoculation, and growth is 48 hours under the vibration of 28 ℃ and 250rpm.
Get the secondary nutrition culture of nutritional medium that the sophisticated fs nutrition of 3500ml culture is used to inoculate the 35L of 50L stirred-tank fermenter, under 28 ℃ and 300rpm condition, cultivated 48 hours, in the stirred-tank fermenter of 500L, fermentation culture keeps cultivating 4 days under 28 ℃ of 300rpm with sophisticated bacterial classification inoculation.The sophisticated fermented product of available suitable solvent extraction, and pass through chromatographic separation and obtain metabolite---sterilant Tianshan Mountains rhzomorph.
Table 2 nutritional medium composition
Composition title content (%)
Glucose 2
Starch 1
Rice meal 1
Yeast extract paste 1
Soybean cake powder 1.5
NaCl??????????????????????????0.5
(NH
4)
2SO
4??????????????????0.5
CaCO
3????????????????????????0.5
pH????????????????????????????7.2
Embodiment two
The present invention during fermentation follows the tracks of the preparation of sterilant Tianshan Mountains rhzomorph by the extract of test broth, for example analyze fermentation broth extract by bioassay method.
Fermentation finishes, fermented product from 500L, mycelia is obtained in method separation with filter press, the acetone soln of mycelia with 1: 2 leached 10 hours, leaching liquid is concentrated remove acetone, use the ethyl acetate extraction working substance again, the acetic acid ethyl acetate extract concentrating under reduced pressure is obtained oily matter, the extraction concentrated solution is mixed silica gel and is crossed post, uses repeatedly silica gel column chromatography and concentrated of ethyl acetate, sherwood oil, obtains the pure product of oily mater.
This compound is an oily liquids, from
1H and
13CNMR molecule as can be known is the compound with long-chain.MSm/z327[M-1]
+, in conjunction with
13The molecular formula that CNMR and DEPT can release this compound is C
19H
36O
4, degree of unsaturation U=2.
IR (KBr) is at 1734cm
-1The place the peak and
13C NMR spectrum illustrates to have a carbonyl in the molecule at the signal that δ 173.6 (C) locates.
13δ 130.5 (CH) in the C NMR spectrum, 128.1 (C) and
1δ 5.3 in the H NMR spectrum (1H) there are one three in t, 5.5Hz and replace two keys in the explanation molecule,
13δ 70.7 (CH) in the CNMR spectrum, 66.1 (CH
2) and 63.9 (CH
2) and
1δ 4.10 in the HNMR spectrum (d, 5Hz, 1H), 4.05 (dd, 6,11Hz, 1H), 3.74 (dd, 6,6Hz, 1H), 3.56 (dd, 5,10Hz, 1H), 3.53 (dd, 6,11Hz, 1H) disclosed existence-OCH in the molecule ,-2 * OCH
2Group;
1The methyl at δ 1.30ppm place is unimodal in the H NMR spectrum, illustrates that it links to each other with quaternary carbon, and
13The quaternary carbon that shows among the CNMR has only one three to replace two keys [δ 128.1 (C)] except that carbonyl, illustrates that this methyl links to each other with double key carbon.Other has two methyl to split to be divided into double peak and coupling constant identical [δ 0.86 (d, 6.5Hz, 6H)], illustrates to have terminal sec.-propyl in the molecule.
Embodiment three, four, five
The contriver has a lot of successful embodiment through secular test, research, shown in act three examples are listed in the table below below:
Table 3
| Measure greatlyyer, the kill pests effect is better. | Less, the kill pests effect is fine. | Seldom, the kill pests effect is fine |
Embodiment six
Sterilant of the present invention Tianshan Mountains rhzomorph is applicable to control insect, mite and tick, and insect, mite and tick amount of suppression are used in the place that is included in insect, mite and tick.Such environment is represented in the place of insect, mite and tick: wherein, the larva of insect, mite and tick, adult and ovum comprise air, food and the object that is contacted.For example, phytophagous insect or mite can be controlled by described active compound being adapted to insect and mite food or parasitic plant part (particularly leaf).The effect of preventing and treating insect by sterilant of the present invention Tianshan Mountains rhzomorph is relevant with access times and using method, and is relevant with the kind of the insect that is prevented and treated simultaneously.
Plantula Brassicae chinensis is potted plant, and when growing to 5 leaves, every basin inserts 50～60 of low age resistance diamondback moth larvaes, waits to try the movable stable back spray of worm preparation of the present invention, handles dead borer population amount, the dead state instituted an inquiry two days later.4 repetitions are established in each processing, calculate mortality ratio, use the result as shown in the table.
The prevention effect of table 4 sterilant Tianshan Mountains rhzomorph antagonism small cabbage moth
| Medicament and concentration | For examination borer population (head/basin) | Handle mortality ratio after 2 days | |
| This compound formulation | ?100ppm | ?52 | ???97.62 |
| ?50ppm | ?49 | ???88.64 | |
| ?25ppm | ?50 | ???78.23 | |
Embodiment seven
As object of reference, detect the insecticidal effect of preparation of the present invention with the water spider of natural propagation growth in the pond.Get the pond water that contains the water spider, add the preparation of the present invention and the crystal Trichlorphon of different concns respectively, establish the control group that does not add medicine simultaneously, observed continuously 3 days, statistical computation preparation of the present invention is to the killing effect of water spider.The use result is as shown in the table.
Table 5 sterilant Tianshan Mountains rhzomorph is to the killing effect of water spider
| Medicine | Concentration (ppm) | Killing effect (%) | ||
| ????24hr | ????48hr | ????72hr | ||
| This compound formulation | ??0.020 | ????88 | ????100 | ????100 |
| ??0.010 | ????81 | ????92 | ????95 | |
| ??0.005 | ????61 | ????72 | ????81 | |
| Trichlorphon | ??0.5 | ????90 | ????92 | ????93 |
Find out that from test-results preparation effect of the present invention is fine, not only antagonism small cabbage moth prevention effect is fine, and also fine to the effect of water spider, and environment is not polluted.Simultaneously, can overcome the resistance of insect to medicament.As the control agent of insect, will help the stable yields and the high yield of agricultural, thereby produce bigger economic and social benefit.
As mentioned above, can realize the present invention preferably.
Claims (7)
1. the bacterial strain of a streptomyces bacterial classification is characterized in that, it is separating obtained from pedotheque, and described bacterial strain Streptomyces sp.GAAS7310 is in China's typical culture collection center preservation, and deposit number is CCTCC NO:M 203058.
2. sterilant Tianshan Mountains rhzomorph is characterized in that it has following molecular formula:
3. the application of a sterilant Tianshan Mountains rhzomorph, it is characterized in that, it is applicable to control mite, tick and other insect, comprises beet armyworm, tobacco budworm, bollworm, small white, Pyrausta nubilalis (Hubern)., small cabbage moth, leaf roller, cutworm, subtropics mythimna separata, rain moth section, India paddy phycitid, Pectinophora gossypiella; Also Coleoptera and weevil are produced effect, comprise colorado potato bug, three chrysomelid, Japanese fox rutelians of cucumber 11 asterophyllite first, cucumber, boll weevil; Also effective to Diptera pest, comprise housefly, Dulicidae, fruit bat, horn fly and Agromyzidae effective; Effective to Anoplura, Isoptera and Hymenoptera ant and mite, comprise citrus red mite, tetranychid; Simultaneously zooparasite there is fine prevention effect, comprises water flea, Chinese anchor head lice.
4. the application of a kind of sterilant according to claim 3 Tianshan Mountains rhzomorph, it is characterized in that, described sterilant Tianshan Mountains rhzomorph is mixed with cream preparation when using, its weight percent proportioning is: sterilant Tianshan Mountains rhzomorph 1～30%, emulsifying agent 5～20%, solvent 50～94%; Process for preparation is: sterilant Tianshan Mountains rhzomorph and synergistic agent and selected tensio-active agent are placed reactor, it at 35 ℃ of temperature, rotating speed reaction 35 minutes under 320 rev/mins the condition, and then the adding organic solvent, continue to stir 35 minutes, get final product after stirring.
5. the application of a kind of sterilant according to claim 4 Tianshan Mountains rhzomorph is characterized in that, sterilant Tianshan Mountains rhzomorph is mixed with cream preparation when using best proportioning is: sterilant Tianshan Mountains rhzomorph 5～10%, emulsifying agent 8～15%, solvent 75～87%.
6. the application of a kind of sterilant according to claim 4 Tianshan Mountains rhzomorph is characterized in that, described emulsifying agent comprises farming breast 2201, agricultural newborn 0203B, YZ960.
7. the application of a kind of sterilant according to claim 4 Tianshan Mountains rhzomorph is characterized in that described solvent comprises Virahol, acetone, dimethyl formamide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031400922A CN1323158C (en) | 2003-08-06 | 2003-08-06 | A insecticide and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031400922A CN1323158C (en) | 2003-08-06 | 2003-08-06 | A insecticide and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1557942A true CN1557942A (en) | 2004-12-29 |
| CN1323158C CN1323158C (en) | 2007-06-27 |
Family
ID=34323798
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031400922A Expired - Fee Related CN1323158C (en) | 2003-08-06 | 2003-08-06 | A insecticide and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1323158C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100497595C (en) * | 2006-09-21 | 2009-06-10 | 广东省农业科学院植物保护研究所 | Extract of soil actinomycetes fermenting liquid and its preparation and application |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305717A (en) * | 2000-12-28 | 2001-08-01 | 广东省农业科学院植物保护研究所 | Process for preparing Tianshan Rhizomorph preparation to prevent and eliminate resistant small plutellid |
-
2003
- 2003-08-06 CN CNB031400922A patent/CN1323158C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100497595C (en) * | 2006-09-21 | 2009-06-10 | 广东省农业科学院植物保护研究所 | Extract of soil actinomycetes fermenting liquid and its preparation and application |
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| Publication number | Publication date |
|---|---|
| CN1323158C (en) | 2007-06-27 |
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