CN1557482A - Immune function regulating and tumor resisting natural nutrient - Google Patents
Immune function regulating and tumor resisting natural nutrient Download PDFInfo
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- CN1557482A CN1557482A CNA2004100161029A CN200410016102A CN1557482A CN 1557482 A CN1557482 A CN 1557482A CN A2004100161029 A CNA2004100161029 A CN A2004100161029A CN 200410016102 A CN200410016102 A CN 200410016102A CN 1557482 A CN1557482 A CN 1557482A
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Abstract
The present invention relates to one kind of natural nutriment for regulating immune functions and resisting tumor, and is especially the preparation process and application of one kind of natural nutriment for regulating immune functions and resisting tumor and rich in lactoferritin and bioactive polypeptide obtained from cow colostrums, milk, whey and relevant product.
Description
Affiliated technical field
The present invention relates to a kind of adjusting immunologic function, the agent of antineoplastic natural nutrition, relate in particular to a kind of white and biologically active polypeptide method immune as regulating, the agent of antitumor natural nutrition of newborn ferrum dawn that from cattle colostrums, milk, serum and Related product thereof, prepares.
The invention technical background
Malignant tumor is the No.1 formidable enemy of human health, the serious harm human health, and there are 8,500,000 newborn tumour patients in the whole world every year approximately, and China has 3,500,000 newborn tumour patients every year approximately.Malignant tumor occupies first of the Chinese cause of death at present.
The generation of human tumor, development and immune function of human body are unbalance certain relation, normally immune surveillance function and the reduction of immune clearance function owing to human body causes tumor growth, therefore, by replenishing the natural nutrition composition of some biologically active, improve immune function of human body, take place significant antineoplastic.
Modern study confirms, white and biologically active polypeptide of the newborn ferrum dawn in the milk has clear and definite mediator's body immunity function, and the enhancing body immunity is to antineoplastic action.
The breast ferrum dawn is that a kind of ferruginous sugar dawn is white in vain, has clear and definite immunoloregulation function, and energy effective stimulus T lymphocyte mainly is to stimulate CD4
+, CD8
+Propagation, maturation; Stimulating interleukin, mainly is to stimulate interleukin 18 (IL-18) to produce, discharge; Stimulating cytokine mainly is the release that stimulates GM-CSF; Activate natural killer cell (NK-Cell) activity; Various immune cell propagations, maturation in the stimulation cycle comprise neutrophil cell, mononuclear cell and macrophage.Zoopery confirms, the breast ferrum dawn can be suppressed all kinds of tumor cells growth in vain effectively and be suppressed the tumor cell diffusion transfer, its dominant mechanism is the absorption tumor cell required iron ion of growth fast, suppress the growth of tumor peripheral vessels simultaneously, new vessels forms around stoping tumor, thereby hinder the tumor tissues blood supply, and by activating the cytotoxic T lymphocyte killing tumor cell.
The milk dawn is in vain after enzyme hydrolysis, generation has the polypeptide of multiple bioactive functions, wherein contain abundant immunoloregulation polypeptide, comprise white f 54-59 of β-cheese dawn and white f 51-53 of α-milky white dawn, white f 191-193 of β-cheese dawn, white f 194-199 of α-cheese dawn, white f 63-68 of β-cheese dawn, Tyr-Gly, Tyr-Gly-Gly, these immunoloregulation polypeptides stimulate human immunocyte's propagation, maturation, improve the ability of immune system antagonism exotic antigen and tumor cell, excite mononuclear cell-macrophage system activity, improve functions of immune system.
The comprehensive document of external report has at present confirmed that white and active polypeptide of newborn ferrum dawn may help to resist other malignant tumor of colon cancer, carcinoma of prostate, gastric cancer and digestive tract, breast carcinoma, skin carcinoma etc.
Milk is one of human main source of nutrition for a long time, and the source is abundant, and low price is safe and reliable, takes nontoxic pair of effect for a long time, prepares to have from milk and clearly regulates immunologic function, and agent is popularized easily to the antineoplastic natural nutrition.
In the technology that European patent (EP-AO 253395) is announced, it is white to have introduced from milk the newborn ferrum dawn of preparation, but this technology is not suitable for heavy industrialization preparation, the also not mentioned active polypeptide for preparing from milk.
Technical problem to be solved by this invention is to prepare white and active polypeptide of newborn ferrum dawn simultaneously from milk, and be applied to mediator's body immunity function, to antitumor.
Summary of the invention
The present invention solves the problems of the technologies described above the technical scheme that is adopted, and it is characterized in that, as raw material, prepares white and active polypeptide of newborn ferrum dawn with cattle colostrums, milk, serum and Related product thereof respectively.Its program and method are, the centrifugal method defat, transfer PH to 4.7～4.8 and add the thermocoagulation cheese dawn white with sour agent, white with the centrifugal or filter type results cheese dawn, it is white that the chromatographic column that supernatant is crossed the cation exchange resin device adsorbs the newborn ferrum dawn, white with the buffer salt solution eluting breast ferrum dawn, the desalination postlyophilization becomes newborn ferrum dawn white lead; Whey liqs molecular weight by chromatographic column is 30,000～40, the supermicro filtration membrane isolated by filtration milk surum dawn of 000D is white, collecting white and cheese dawn at dawn of ultrafiltration and concentration merges in vain, after adopting the white enzyme hydrolysis of compound dawn, with molecular weight is 10, is spray dried to the active polypeptide powder after the supermicro filtration membrane isolated by filtration of 000D.Use newborn ferrum dawn white lead or active polypeptide powder separately, perhaps The combined is used, and preparation various ways food or medicament are with mediator's body immunity function, to antitumor.
Prepare the white and used milk class raw material of active polypeptide of newborn ferrum dawn, it can be cattle colostrums, milk, also can be caseic pair of product serum of preparation, it perhaps is the serum powder of feedstock production with the serum, serum concentrates dawn white lead (WPC), the white powder (WPI) that separates of serum dawn, in order to keep its biological activity, the raw material of preferably selecting for use is not crossed by high-temperature process, the temperature of pasteurization had better not surpass 72 ℃ and continue 15 minutes, perhaps 63 ℃ continue 30 minutes, otherwise the biological activity of most bioactive substance (the newborn ferrum dawn is white) will be lost, optimal raw material is a cattle colostrums, but because the cattle colostrums source is less relatively, during large-scale industrial production, adopting milk as raw material, also is a kind of feasible selection.
Technical characterictic of the present invention is, the milk of collecting need be removed fat in the milk through the defat program, usually adopt known centrifugation defat, add sour agent in defat cattle colostrums or the milk and regulate pH value to 4.6～4.8, heating 30 ℃～40 ℃, to continue to solidify in 20～30 minutes the cheese dawn white, and the cheese dawn of having condensed collects by centrifugation in vain or collects standby by the filtering method of filter cloth.
Technical characterictic of the present invention is, defat and removal cheese dawn, white whey liqs passed through the chromatographic column of cation exchange resin fill, when liquid is crossed the post rate by chromatographic column with higher superficial velocity with higher liquid, the cationic exchange resin adsorption breast ferrum dawn is white, after crossing the end of post process, be adsorbed on dawn white matter on the cation exchange resin with the saline solution eluting, concentrate the eluate after drying, can prepare newborn white flour of ferrum dawn.
Technical characterictic of the present invention is, can adopt polytype cation exchange resin, but preferably adopt the product that under high pressure high flow rate situation, still can keep the physical mechanical stability, agarose gel (pharmacy grade) for example, just meet above-mentioned condition, the cation exchange resin granular size scope of selecting is 100.mu.m～300.mu.m, and preferably scope is 125.mu.m～150.mu.m, can provide high surfaces speed when absorbing.
Technical characterictic of the present invention is, ion exchange resin is preferably at first used the phosphate buffer (0.025molNa.sub2 PHO.sub.4/NaH.sub2 PO.sub.4) of PH6.5, approximately adopt the buffer flushing chromatographic column of 4 times of bed capacity to be enough to balance, at cattle colostrums, milk or serum are by behind the chromatographic column, the breast ferrum dawn is attracted on the cation exchange resin in vain, but the material that the chromatographic column of reuse buffer salt solution flushing at this moment eluting has adsorbed, about 0.7～1.5mol the NaCL of the buffer concentration of elution chromatography post, eluent can desalination, also can convection drying, the desalination mode comprises ion exchange, electrodialysis or ultrafiltration, ultrafiltration is the most first-selected desalination mode, and to adopt freeze-drying method to prepare newborn ferrum dawn white spirit powder standby for eluent behind the desalination.
Technical characterictic of the present invention is, collect whey by chromatographic column, adopt staggered tangent plane fluid ultrafiltration apparatus, using molecular weight is 30,000～40, and the supermicro filtration membrane of 000D carries out isolated by filtration, separate micromolecular lactose, mineral, residual fat, contain multiple dawn white matter in the ultrafiltration concentrate, comprise that β-milk-globule dawn is white, α-milky white dawn white, immunoglobulin etc., the collection ultrafiltration concentrate is further handled.
Technical characterictic of the present invention is, it is white that the milk surum dawn white and ultra-filtration and separation at sedimentary cheese dawn has been solidified in the merging collection, it is white to add alkali liquor (for example 1N NaOH) adjusting PH to 6.5～6.8 dissolving cheese dawn, solution includes dawn white matter 10 grams～60 grams by diluting or being concentrated into every liter of liquid, preferably 40 restrain～60 grams per liters, the mixture that in white solution of compound dawn, adds dan white pancreatic enzyme and pancreas curdled milk white enzyme of dawn, the dan white pancreatic enzyme amount is equal to or greater than 800USP unit/mg, preferably be equal to or greater than 1000US unit/mg, preferably be equal to or greater than 1800US unit/mg, the pancreas curdled milk dawn, white enzyme amount was equal to or greater than 150USP unit/mg, preferably be equal to or greater than 200USP unit/mg, preferably be equal to or greater than 350USP unit/mg.
Technical characterictic of the present invention is, mix the dawn when white in hydrolysis, the pancreas dawn of calculating with above-mentioned USP unit in dan white pancreatic enzyme and the white enzymatic mixture of pancreas curdled milk dawn is white/ratio about 1～18 of white enzyme of pancreas curdled milk dawn, be chosen as 1.5～12 preferably, best choice is 4～6, the consumption of enzymatic mixture accounts for 0.4～1.2% (percentage by weight) of white total amount of hydrolysis dawn, preferably 0.6～0.8% (percentage by weight).
Technical characterictic of the present invention is, the process of the above-mentioned white mixed liquor of dawn of hydrolysis is, being heated to 75 ℃～85 ℃ kept 10～30 minutes, being cooled to 30 ℃～50 ℃ kept 2～8 hours, hydrolysis period P H maintains between 6～8, preferably between 6.5～6.8, when hydrolytic process finishes, be heated to 80 ℃～90 ℃, best 85 ℃ continue 10 minutes, make enzyme-deactivating by heat treatment, can also make enzyme-deactivating by ultra high temperature sterilization (UHTS) (temperature was kept 30 seconds for 130 ℃), the solution molecular weight after dawn plain boiled water is separated is that 10,000 supermicro filtration membrane is in staggered tangent plane fluid ultrafiltration apparatus isolated by filtration, remove macromole, the unhydrolysed dawn is white, and the direct spray drying of ultrafiltrate becomes the active polypeptide powder.
Technical characterictic of the present invention is, prepare the method that the newborn ferrum dawn is white and active polypeptide can also adopt another kind to improve, promptly omit white coagulation program of cheese dawn, with the cattle colostrums or the milk of defat is 30 with molecular weight directly, 000～40, the supermicro filtration membrane of 000D separates the lactose of small-molecular weight, mineral, behind the fat-soluble composition, it is white that direct chromatographic column excessively adsorbs the newborn ferrum dawn, and directly carrying out dawn plain boiled water, the liquid by chromatographic column separates program, in vain to separate program technic identical with dawn plain boiled water to separate the newborn ferrum dawn, using milk surum (product is paid in cheese production), whey powder, milk surum dawn white lead etc. also should adopt this program and method to prepare newborn ferrum dawn and active polypeptide during as raw material.
Technical characterictic of the present invention is, adopt newborn ferrum dawn of the inventive method preparation white and active polypeptide both can be applied to mediator's body immunity function separately separately, to antitumor, also can use in conjunction, preferably The combined is used, the performance complementary action, during use in conjunction, both can unitary agent unite and took, and also can two kinds of compositions be prepared into compound formulation and take.
Technical characterictic of the present invention is, white and active polypeptide of the newborn ferrum dawn of the inventive method preparation, can be applied to multiple enhancing immune function of human body and in the agent of antineoplastic natural nutrition, can allow tumor high-risk group or have the crowd of tumor family history to take, regulate immunologic function, prophylaxis of tumours, also can be applied to multiple cancer patients such as colon cancer, carcinoma of prostate, gastric cancer and other digestive tract tumor, breast carcinoma, skin carcinoma, use the cancer patient who carries out chemotherapy, radiotherapy behind all kinds of excisions, raise immunity, performance is to the antineoplastic synergism.
Technical characterictic of the present invention is, mediator's body immunity function of the inventive method preparation and to the antineoplastic breast ferrum dawn white and active polypeptide, can be prepared into multiple food form or medicament forms uses, main application mode is oral, can directly directly take with mixing in water for oral taking or with the concentrated solution form with dry powder form, also can be used as food additive is added in the food and uses, include but not limited to food forms such as milk powder, condensed milk, instant, instant tea, cookies, cake, confection, Mel; Also can mix and prepare various medicine types and use, include but not limited to medicine types such as tablet, capsule, pill, powder, granule, electuary, Emulsion, soft capsule, oral liquid, spray with various medical-grade adjuvants.
The invention has the beneficial effects as follows, prepare multiple mediator's body immunity function simultaneously from natural material, to the agent of antineoplastic natural nutrition, can take for a long time, nontoxic pair of effect strengthens immune function of human body in effectively to antitumor cell.
The specific embodiment
The invention will be further described below in conjunction with embodiment:
Embodiment 1
White and active polypeptide of newborn ferrum dawn of preparation from cattle colostrums
Collect cow and divide 15 liters of cattle colostrumss in three days puerperiums, adopt the centrifugation defat, 5000 rev/mins of centrifugal speeds, centrifugation time 20 minutes, the defat cattle colostrums is by staggered tangent plane fluid separating and filtering device (Pall company produces the TFF device), selecting the supermicro filtration membrane molecular weight is 40, and 000D collects remaining concentrate and prepared chromatographic column.
Adopt 10cm to control the chromatograph (Bioprocess system pharmacia) of production automatically, device agarose gel cation exchange resin (SP Sepharose Big Beadsion exchanger), the chromatographic column diameter is 10cm, and bed height is 11cm, and the bed capacity is 0.864 liter.
At room temperature, with 3.2 liters of 0.025M, the PH6.5 phosphate buffer carries out balance to chromatographic column, and ultrafiltration concentrate is taken out mode with pump, passes through chromatographic column with 180 liters/hour flow velocitys, and the about 2300cm/ of superficial velocity hour, about 208 capacity of mistake post speed/hour.
With the flushing of 1.0M NaCL buffer, the flushing liquid measure is 2.2 liters, obtains newborn white eluent of ferrum dawn, and two kinds of eluents use 10 respectively, and the supermicro filtration membrane ultrafiltration desalination of 000D concentrates postlyophilization, obtains newborn ferrum dawn white spirit powder.
Collected the cattle colostrums of chromatographic column, heat ℃ dissolving of white solution to 50 of dawn and reach 4～6% dawn white matter concentration, heat and kept 30 minutes down to 75 ℃, add dan white pancreatic enzyme and white enzymatic mixture of pancreas curdled milk dawn when being cooled to 50 ℃, enzymatic mixture weight accounts for white gross weight 0.8% of dawn, and (dan white pancreatic enzyme is 1800USP unit/mg, white enzyme of pancreas curdled milk dawn is 350USP unit/mg, available from Novo Nordisk Bioindustrids Inc.Danbury.CT.U.S.A., the ratio of the USP unit of dan white pancreatic enzyme/pancreas curdled milk white enzyme of dawn is 5), holding temperature kept 6 hours for 50 ℃, PH is adjusted in 6.5, before hydrolysis finishes, inject 130 ℃ of 45 seconds of steam in hydrolyzate, kill bacteria makes enzyme-deactivating simultaneously.
Having finished the white hydrolyzed solution of dawn that dawn plain boiled water separates process is 10 with the staggered tangent plane fluid ultrafiltration apparatus molecular weight of vacuum pump suction, and the supermicro filtration membrane separating and filtering of 000D is collected filtering supernatant and directly is spray dried to active polypeptide dry powder.
Embodiment 2
Measure the active polypeptide molecular weight
Get the active polypeptide powder of embodiment 1 preparation, with size sieve chromatography method (SEC), measure the active polypeptide molecular weight distribution, in TSK G-2000SWXL post, polypeptide is separated by molecular dimension, measure the absorbability of 214nm with the UV detector, and with the polypeptide curve ratio of itself and known molecular amount.
Molal weight % molecular weight distribution
(g/mlo)
MM＞5000 7.8％
5000＞MM＞3000 13.7％
3000＞MM＞2000 14.6％
2000＞MM＞1000 25.8％
1000＞MM＞500 18.9％
MM＞500 19.2％
Claims (10)
1. the present invention relates to a kind of adjusting immunologic function, the agent of antineoplastic natural nutrition, relate in particular to a kind of white and biologically active polypeptide method immune as regulating, the agent of antitumor natural nutrition of newborn ferrum dawn that from cattle colostrums, milk, serum and Related product thereof, prepares, it is characterized in that, as raw material, prepare white and active polypeptide of newborn ferrum dawn with cattle colostrums, milk, serum and Related product thereof respectively.Its program and method are, the centrifugal method defat, transfer PH to 4.7～4.8 and add the thermocoagulation cheese dawn white with sour agent, white with the centrifugal or filter type results cheese dawn, it is white that the chromatographic column that supernatant is crossed the cation exchange resin device adsorbs the newborn ferrum dawn, white with the buffer salt solution eluting breast ferrum dawn, the desalination postlyophilization becomes newborn ferrum dawn white lead; Whey liqs molecular weight by chromatographic column is 30,000～40, the supermicro filtration membrane isolated by filtration milk surum dawn of 000D is white, collecting white and cheese dawn at dawn of ultrafiltration and concentration merges in vain, after adopting the white enzyme hydrolysis of compound dawn, with molecular weight is 10, is spray dried to the active polypeptide powder after the supermicro filtration membrane isolated by filtration of 000D.Use newborn ferrum dawn white lead or active polypeptide powder separately, perhaps The combined is used, and preparation various ways food or medicament are with mediator's body immunity function, to antitumor.
2. in accordance with the method for claim 1, technical characterictic of the present invention is, defat and removal cheese dawn, white whey liqs passed through the chromatographic column of cation exchange resin fill, when liquid is crossed the post rate by chromatographic column with higher superficial velocity with higher liquid, the cationic exchange resin adsorption breast ferrum dawn is white, after crossing the post process and finishing, is adsorbed on dawn white matter on the cation exchange resin with the saline solution eluting, concentrate the eluate after drying, can prepare newborn white flour of ferrum dawn.
3. in accordance with the method for claim 1, technical characterictic of the present invention is, ion exchange resin is preferably at first used the phosphate buffer (0.025mol Na.sub2 PHO.sub.4/NaH.sub2 PO.sub.4) of PH6.5, approximately adopt the buffer flushing chromatographic column of 4 times of bed capacity to be enough to balance, at cattle colostrums, milk or serum are by behind the chromatographic column, the breast ferrum dawn is attracted on the cation exchange resin in vain, but the material that the chromatographic column of reuse buffer salt solution flushing at this moment eluting has adsorbed, about 0.7～1.5mol the NaCL of the buffer concentration of elution chromatography post, eluent can desalination, also can convection drying, the desalination mode comprises ion exchange, electrodialysis or ultrafiltration, ultrafiltration is the most first-selected desalination mode, and to adopt freeze-drying method to prepare newborn ferrum dawn white spirit powder standby for eluent behind the desalination.
4. in accordance with the method for claim 1, technical characterictic of the present invention is, collection is adopted staggered tangent plane fluid ultrafiltration apparatus by the whey of chromatographic column, and using molecular weight is 30,000～40, the supermicro filtration membrane of 000D carries out isolated by filtration, separates micromolecular lactose, mineral, residual fat, contains multiple dawn white matter in the ultrafiltration concentrate, comprise that β-milk-globule dawn is white, α-milky white dawn white, immunoglobulin etc., the collection ultrafiltration concentrate is further handled.
5. in accordance with the method for claim 1, technical characterictic of the present invention is, it is white that the milk surum dawn white and ultra-filtration and separation at sedimentary cheese dawn has been solidified in the merging collection, it is white to add alkali liquor (for example 1N NaOH) adjusting PH to 6.5～6.8 dissolving cheese dawn, solution includes dawn white matter 10 grams～60 grams by diluting or being concentrated into every liter of liquid, preferably 40 restrain～60 grams per liters, the mixture that in white solution of compound dawn, adds dan white pancreatic enzyme and pancreas curdled milk white enzyme of dawn, the dan white pancreatic enzyme amount is equal to or greater than 800USP unit/mg, preferably be equal to or greater than 1000US unit/mg, preferably be equal to or greater than 1800US unit/mg, the pancreas curdled milk dawn, white enzyme amount was equal to or greater than 150USP unit/mg, preferably be equal to or greater than 200USP unit/mg, preferably be equal to or greater than 350USP unit/mg.
6. in accordance with the method for claim 1, technical characterictic of the present invention is, mix the dawn when white in hydrolysis, the pancreas dawn of calculating with above-mentioned USP unit in dan white pancreatic enzyme and the white enzymatic mixture of pancreas curdled milk dawn is white/ratio about 1～18 of white enzyme of pancreas curdled milk dawn, be chosen as 1.5～12 preferably, best choice is 4～6, and the consumption of enzymatic mixture accounts for 0.4～1.2% (percentage by weight) of white total amount of hydrolysis dawn, preferably 0.6～0.8% (percentage by weight).
7. in accordance with the method for claim 1, technical characterictic of the present invention is, prepare the method that the newborn ferrum dawn is white and active polypeptide can also adopt another kind to improve, promptly omit white coagulation program of cheese dawn, with the cattle colostrums or the milk of defat is 30 with molecular weight directly, 000～40, the supermicro filtration membrane of 000D separates the lactose of small-molecular weight, mineral, behind the fat-soluble composition, it is white that direct chromatographic column excessively adsorbs the newborn ferrum dawn, and directly carrying out dawn plain boiled water, the liquid by chromatographic column separates program, in vain to separate program technic identical with dawn plain boiled water to separate the newborn ferrum dawn, using milk surum (product is paid in cheese production), whey powder, milk surum dawn white lead etc. also should adopt this program and method to prepare newborn ferrum dawn and active polypeptide during as raw material.
8. in accordance with the method for claim 1, technical characterictic of the present invention is, the process of the above-mentioned white mixed liquor of dawn of hydrolysis is, being heated to 75 ℃～85 ℃ kept 10～30 minutes, being cooled to 30 ℃～50 ℃ kept 2～8 hours, hydrolysis period P H maintains between 6～8, preferably between 6.5～6.8, when hydrolytic process finishes, be heated to 80 ℃～90 ℃, best 85 ℃ continue 10 minutes, make enzyme-deactivating by heat treatment, can also make enzyme-deactivating by ultra high temperature sterilization (UHTS) (temperature was kept 30 seconds for 130 ℃), solution molecular weight after dawn plain boiled water is separated is 10,000 supermicro filtration membrane is removed macromole in staggered tangent plane fluid ultrafiltration apparatus isolated by filtration, the unhydrolysed dawn is white, and the direct spray drying of ultrafiltrate becomes the active polypeptide powder.
9. in accordance with the method for claim 1, technical characterictic of the present invention is, white and active polypeptide of the newborn ferrum dawn of the inventive method preparation, can be applied to multiple enhancing immune function of human body and in the agent of antineoplastic natural nutrition, can allow tumor high-risk group or have the crowd of tumor family history to take, regulate immunologic function, prophylaxis of tumours, also can be applied to colon cancer, carcinoma of prostate, gastric cancer and other digestive tract tumor, breast carcinoma, multiple cancer patient such as skin carcinoma, carry out chemotherapy after using all kinds of excisions, the cancer patient of radiotherapy, raise immunity, performance is to the antineoplastic synergism.
10. in accordance with the method for claim 1, technical characterictic of the present invention is, mediator's body immunity function of the inventive method preparation and to the antineoplastic breast ferrum dawn white and active polypeptide, can be prepared into multiple food form or medicament forms uses, main application mode is oral, can directly directly take with mixing in water for oral taking or with the concentrated solution form with dry powder form, also can be used as food additive is added in the food and uses, include but not limited to food forms such as milk powder, condensed milk, instant, instant tea, cookies, cake, confection, Mel; Also can mix and prepare various medicine types and use, include but not limited to medicine types such as tablet, capsule, pill, powder, granule, electuary, Emulsion, soft capsule, oral liquid, spray with various medical-grade adjuvants.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100161029A CN1557482A (en) | 2004-02-04 | 2004-02-04 | Immune function regulating and tumor resisting natural nutrient |
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| CNA2004100161029A CN1557482A (en) | 2004-02-04 | 2004-02-04 | Immune function regulating and tumor resisting natural nutrient |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1875748B (en) * | 2006-03-15 | 2011-07-06 | 杨林伟 | Application method of lactoferritin in food |
| CN102172360A (en) * | 2011-02-17 | 2011-09-07 | 南京大学 | Colostrum extract and extraction method and application thereof |
| CN101272694B (en) * | 2005-07-29 | 2012-04-18 | 欧洲乳制品公司 | Novel milk protein fractions and use thereof for preventing or treating chronic inflammatory diseases |
| CN102850430A (en) * | 2011-06-29 | 2013-01-02 | 中国科学院上海生命科学研究院 | Animal colostrum derived protein fragment and application thereof |
| CN105998070A (en) * | 2016-06-29 | 2016-10-12 | 广州联存医药科技股份有限公司 | Immunity-enhancing preparation prepared by taking bovine colostrum powder and milk mineral salts as raw materials and preparation method for immunity-enhancing preparation |
| CN107625957A (en) * | 2009-10-22 | 2018-01-26 | 普罗潘克股份有限公司 | The pharmaceutical composition for being used to treat cancer comprising trypsinogen and chymotrypsinogen |
-
2004
- 2004-02-04 CN CNA2004100161029A patent/CN1557482A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101272694B (en) * | 2005-07-29 | 2012-04-18 | 欧洲乳制品公司 | Novel milk protein fractions and use thereof for preventing or treating chronic inflammatory diseases |
| CN1875748B (en) * | 2006-03-15 | 2011-07-06 | 杨林伟 | Application method of lactoferritin in food |
| CN107625957A (en) * | 2009-10-22 | 2018-01-26 | 普罗潘克股份有限公司 | The pharmaceutical composition for being used to treat cancer comprising trypsinogen and chymotrypsinogen |
| CN107625957B (en) * | 2009-10-22 | 2021-06-15 | 普罗潘克股份有限公司 | Pharmaceutical composition for treating cancer comprising trypsinogen and chymotrypsinogen |
| CN102172360A (en) * | 2011-02-17 | 2011-09-07 | 南京大学 | Colostrum extract and extraction method and application thereof |
| CN102172360B (en) * | 2011-02-17 | 2013-04-24 | 南京大学 | Colostrum extract and extraction method and application thereof |
| CN102850430A (en) * | 2011-06-29 | 2013-01-02 | 中国科学院上海生命科学研究院 | Animal colostrum derived protein fragment and application thereof |
| CN105998070A (en) * | 2016-06-29 | 2016-10-12 | 广州联存医药科技股份有限公司 | Immunity-enhancing preparation prepared by taking bovine colostrum powder and milk mineral salts as raw materials and preparation method for immunity-enhancing preparation |
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