CN1553168A - Capillary array electrophoretic ratating laser scanning co-focusing fluorescent inspecting instrument - Google Patents
Capillary array electrophoretic ratating laser scanning co-focusing fluorescent inspecting instrument Download PDFInfo
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- CN1553168A CN1553168A CNA03133511XA CN03133511A CN1553168A CN 1553168 A CN1553168 A CN 1553168A CN A03133511X A CNA03133511X A CN A03133511XA CN 03133511 A CN03133511 A CN 03133511A CN 1553168 A CN1553168 A CN 1553168A
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- kapillary
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Abstract
An electrophoresis apparatus which is composed of laser, high speed DC motor, rotary coder, high voltage power supply, capillary, buffer solution, reflector and detector is arranged as the follows, capillary is laid out in round shape of symmetric array, high speed DC motor is applied to quicken data collection speed and rotary coder is applied to confirm position of each capillary accurately, rotary reflector is set on central axle of round column on capillary, its reflection face and central axle are presented as of 45 degree angle and laser is focused on capillary, fluorescent signal excited on capillary is transmitted to detector along with light path.
Description
Technical field
The present invention relates to a kind of capillary array electrophoresis instrument, specifically a kind of capillary array electrophoresis rotary laser scanning confocal fluorescent detector.
Background technology
Along with the development of combinatorial chemistry, hundreds and thousands of kinds medicine was synthesized out in one day, and characterizing and analyze these medicines needs high-throughout instrument and method, requires the development of high-throughout medicine and bioassay technique.The application of Capillary Electrophoresis aspect analytical chemistry is increasingly extensive, detection to inorganic ions and organic-biological all has report, become important analysis tool, Capillary Electrophoresis have analysis speed fast, consume the characteristics that sample size is few and separation efficiency is high, because size capillaceous is suitable for multichannel analysis, so the capillary array electrophoresis technology grows up gradually.
The capillary array electrophoresis instrument is mainly used in the dna sequencing aspect at present, the laser Induced Fluorescence Technology that adopt detect more, laser Induced Fluorescence Technology is divided into two kinds in the application of capillary array electrophoresis owing to the difference of detection mode, a kind ofly be that imaging type detects, adopt beam of laser to shine simultaneously on all array capillaries, adopt the large scale lens simultaneously the fluorescence signal of sample separation to be passed to detecting device; The another kind detection mode is that scan-type detects, and adopts laser by root scanning kapillary, with the fluorescence signal transmission detecting device of every capillary.These two kinds of methods respectively have relative merits, the laser intensity that imaging type detects and requires the laser intensity height, shines on every capillary requires identical, scan-type then needs mechanical mobile laser or array capillary, the mechanical technique that control comes and goes translation requires high, requirement has high degree of accuracy, and the speed of scan-type image data is slower.
Summary of the invention
The object of the present invention is to provide a kind of capillary array electrophoresis rotary laser scanning confocal fluorescent detector of fast a large amount of reading of data.
For achieving the above object, the technical solution used in the present invention is:
Mainly by laser instrument, the twinax high-speed direct current generator, rotary encoder, high-voltage power supply, kapillary, buffer pool, catoptron and detecting device are formed, wherein the kapillary two ends place buffer pool respectively, and link to each other with two electrodes of high-voltage power supply, the rounded symmetrical distribution of kapillary, employing twinax high-speed direct current generator is accelerated acquisition speed and is accurately located the position of every capillary with rotary encoder, rotating mirror places on the cylindrical center capillaceous axle, its reflecting surface and central shaft are miter angle, catoptron on the central shaft can rotate relatively with the kapillary of cylindric distribution, on kapillary, the excited fluorescent signal is delivered to detecting device through light path to the laser that laser instrument sends on the kapillary through focusing mirror.
The concrete structure composition can be:
One cylinder ring, it is fixed on the supporting seat, is evenly equipped with aperture on it, and the aperture that kapillary passes on it is fixed;
One twin shaft direct current generator, it is fixed on the supporting seat on the top of cylinder ring, external power supply, the catoptron that is coupling down, last axle connects a rotary encoder; Rotary encoder passes to computing machine with the capillary position signal through decoding scheme, data collecting card;
One dichroic mirror, it is positioned at catoptron below and parallel with catoptron, is provided with lens between catoptron and dichroic mirror, laser through dichroic mirror, lens focus on kapillary;
One photomultiplier, fluorescence signal arrives dichroic mirror through catoptron and the burnt reflection of lens copolymerization, through second lens, pass to photomultiplier behind sheet, diaphragm and the optical filter, photomultiplier passes to data collecting card with signal again, and data collecting card passes to computing machine with signal.
Be preferably in for ease of installation and be provided with second catoptron between laser instrument and dichroic mirror, the laser that laser instrument sends passes to dichroic mirror through second catoptron; Install a hollow circuit cylinder on supporting seat additional, cylinder ring is sheathed on the hollow circuit cylinder, and the twin shaft direct current generator is fixed in the hollow circuit cylinder.
Preferably adopt adjustable speed twinax high-speed direct current generator in this device, maximum speed is 4000 rev/mins; Adopt rotary encoder accurately to locate the position of every capillary, the resolution of rotary encoder is high more, the capillary number that can improve accurate location is just many more, preferably adopt high-resolution absolute rotary encoder, determine that according to the signal of scrambler more capillaries can be located in the position of every capillary; Laser instrument can adopt continuous laser.
The present invention has following advantage:
1. high throughput analysis sample.The capillary array electrophoresis instrument can high flux, parallel separation detection sample, and many capillaries of compartment analysis simultaneously, promptly high-throughout sample analysis once can detect thousands of capillaries simultaneously; The consumption sample size is few, separation efficiency is high; The present invention adopts new scan-type capillary array to detect, overcome the shortcoming (for example sweep velocity shakiness, slow the leakage easily of detection data speed are adopted data) of scan-type detection mode, take the capillary array of circular symmetry, adopt the high speed direct current generator to combine and locate position capillaceous with the rotation code translator, the detection mode that adopts is rotary confocal laser induced fluoroscopic examination, acquisition speed is fast, and a kind of high flux sample separation, capillary array electrophoresis device are provided.
2. kapillary accurate positioning, acquisition speed is fast, degree of accuracy is high.The combination of direct current generator and scrambler has the image data characteristic of accurate, and the umber of pulse that scrambler sends is consistent with number of capillaries, data of every pulse collection, and leak source and dislocation have been avoided in accurate positioning.
3. automaticity height.High speed direct current generator and rotary encoder are applied to the capillary array electrophoresis instrument, have strengthened the robotization of capillary array electrophoresis instrument.
Description of drawings
Fig. 1 is the schematic diagram of apparatus of the present invention;
Fig. 2 is the concrete pictorial diagram of the present invention;
Fig. 3 is the figure as a result that the experiment of 8 passages separates.
Embodiment
Embodiment
The main laser instrument 1 of whole device, high-voltage power supply 2, kapillary 3, first buffer pool 4, second buffer pool 5, first electrode 6, second electrode 7, catoptron 8, second catoptron 24, dichroic mirror 9, lens 10, second lens 26, optical filter 11, diaphragm 12, photomultiplier 13, stabilized voltage supply 14, high speed twin shaft direct current generator 15, rotary encoder 16, support bar 17, motor supporting seat 18, hollow circuit cylinder 19, the foraminate garden of the symmetry circle distribution band of column 20, decoding scheme 21, data collecting card 22, computing machine 23, form by sheet 25.
The capillary array detector of present embodiment 8 passages, its split tunnel be kapillary 3,8 capillaries 3 circular symmetric offset spread on the garden band of column 20,15, one in twin shaft direct current generator another axle that links to each other with catoptron or lens is flexible coupling with rotary encoder 16; Use lens 10, second lens 26, catoptron 8, dichroic mirror 9 to constitute focusing laser-Induced Fluorescence Detection altogether; Utilize the sampling trigger signal of the signal of rotary encoder 16 outputs, can carry out high flux, parallel compound separation and detection as data collecting card 22; Sample is the amino acid of 4 kinds of mixing, uses marked by fluorescein isothiocyanate, adopts the capillary sample introduction 10 seconds, and dissociating buffer is 10mM borate buffer (PH=10 contains 5mM γ cyclodextrin), separation voltage 6.4KV; Separating resulting is seen Fig. 3: wherein X-axis is sample time of flowing out (unit for minute), Y-axis is port number (being 8 passages altogether), the Z axle is the peak height of signal, and the order that each passage flows out the sample peak is followed successively by lysine, fluorescein isothiocyanate, tyrosine, glutamic acid, aspartic acid (delivery time increases successively).
Claims (5)
1. the capillary array electrophoresis rotary laser scans the confocal fluorescent detector, mainly by laser instrument (1), high-voltage power supply (2), kapillary (3), buffer pool, catoptron (8), high speed twin shaft direct current generator (15), rotary encoder (16) and detecting device are formed, it is characterized in that: kapillary (3) two ends place buffer pool respectively, and link to each other with two electrodes of high-voltage power supply (2), the rounded symmetrical distribution of kapillary (3), employing twinax high-speed direct current generator (15) is accelerated acquisition speed and is accurately located the position of every capillary with rotary encoder (16), catoptron (8) places on cylindrical center's axle of kapillary (3), its reflecting surface and central shaft are miter angle, catoptron on the central shaft (8) can with the relative rotation of kapillary (3) of cylindric distribution, the laser that laser instrument (1) sends focuses on the kapillary (3) through catoptron (8), and kapillary (3) is gone up the excited fluorescent signal and is delivered to detecting device through light path.
2. by the described fluorescence detector of claim 1, it is characterized in that:
One cylinder ring (20), it is fixed on the supporting seat (18), is evenly equipped with aperture on it, and the aperture that kapillary (3) passes on it is fixed;
One twin shaft direct current generator (15), it is on the top of cylinder ring (20), be fixed on the supporting seat (18), external power supply (14), under the catoptron (8) that is coupling, last axle connects a rotary encoder (16), and rotary encoder (16) passes to computing machine (23) with the capillary position signal through decoding scheme (21), data collecting card (22);
One dichroic mirror (9), it is positioned at catoptron (8) below and parallel with catoptron (8), is provided with lens (10) between catoptron (8) and dichroic mirror (9), and laser focuses on the kapillary (3) through dichroic mirror (9), lens (10);
One photomultiplier (13), fluorescence signal arrives dichroic mirror (9) through catoptron (8) and the burnt reflection of lens (10) copolymerization, again through second lens (26), pass to photomultiplier (13) behind sheet (25), diaphragm (12) and the optical filter (11), photomultiplier (13) passes to data collecting card (22) with signal, and data collecting card (22) passes to computing machine (23) with signal.
3. by the described fluorescence detector of claim 2, it is characterized in that: be provided with second catoptron (24) between laser instrument (1) and dichroic mirror (9), the laser that laser instrument (1) sends passes to dichroic mirror (9) through second catoptron (24).
4. by the described fluorescence detector of claim 2, it is characterized in that: install a hollow circuit cylinder (19) on supporting seat (18) additional, cylinder ring (20) is sheathed on the hollow circuit cylinder (19), and twin shaft direct current generator (15) is fixed in the hollow circuit cylinder (19).
5. by the described fluorescence detector of claim 1, it is characterized in that: laser instrument (1) adopts continuous laser.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133511 CN1235038C (en) | 2003-05-28 | 2003-05-28 | Capillary array electrophoretic ratating laser scanning co-focusing fluorescent inspecting instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133511 CN1235038C (en) | 2003-05-28 | 2003-05-28 | Capillary array electrophoretic ratating laser scanning co-focusing fluorescent inspecting instrument |
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| Publication Number | Publication Date |
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| CN1553168A true CN1553168A (en) | 2004-12-08 |
| CN1235038C CN1235038C (en) | 2006-01-04 |
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| CN 03133511 Expired - Fee Related CN1235038C (en) | 2003-05-28 | 2003-05-28 | Capillary array electrophoretic ratating laser scanning co-focusing fluorescent inspecting instrument |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100432654C (en) * | 2006-03-30 | 2008-11-12 | 上海大学 | Integrated capillary electrophoretic chip scanning analysis system and method |
| CN100543460C (en) * | 2007-05-24 | 2009-09-23 | 泰州动态通量生命科学仪器有限公司 | A kind of high flux real-time minimum multifunctional fluorescent detector |
| CN101165472B (en) * | 2006-10-20 | 2010-05-12 | 中国科学院大连化学物理研究所 | Multiple-pass mode capillary tube electrophoresis device |
| CN102331415A (en) * | 2011-06-15 | 2012-01-25 | 公安部第一研究所 | Method for positioning capillary tube array by using raman spectral imaging |
| WO2014183468A1 (en) * | 2013-05-13 | 2014-11-20 | Yan Chao | Serial dual-optical path laser-induced fluorescence spectrometer |
| CN106018403A (en) * | 2016-05-12 | 2016-10-12 | 刘马禾 | Light absorption detector of array capillary electrophoresis apparatus and detection method |
| CN108181239A (en) * | 2018-02-07 | 2018-06-19 | 张哲夫 | A kind of optical system of multichannel fluorescence quantitative PCR instrument |
-
2003
- 2003-05-28 CN CN 03133511 patent/CN1235038C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100432654C (en) * | 2006-03-30 | 2008-11-12 | 上海大学 | Integrated capillary electrophoretic chip scanning analysis system and method |
| CN101165472B (en) * | 2006-10-20 | 2010-05-12 | 中国科学院大连化学物理研究所 | Multiple-pass mode capillary tube electrophoresis device |
| CN100543460C (en) * | 2007-05-24 | 2009-09-23 | 泰州动态通量生命科学仪器有限公司 | A kind of high flux real-time minimum multifunctional fluorescent detector |
| CN102331415A (en) * | 2011-06-15 | 2012-01-25 | 公安部第一研究所 | Method for positioning capillary tube array by using raman spectral imaging |
| CN102331415B (en) * | 2011-06-15 | 2014-06-04 | 公安部第一研究所 | Method for positioning capillary tube array by using raman spectral imaging |
| WO2014183468A1 (en) * | 2013-05-13 | 2014-11-20 | Yan Chao | Serial dual-optical path laser-induced fluorescence spectrometer |
| CN106018403A (en) * | 2016-05-12 | 2016-10-12 | 刘马禾 | Light absorption detector of array capillary electrophoresis apparatus and detection method |
| CN106018403B (en) * | 2016-05-12 | 2019-05-21 | 南京擎科生物科技有限公司 | The light absorption detector and detection method of capillary array electrophoresis instrument |
| CN108181239A (en) * | 2018-02-07 | 2018-06-19 | 张哲夫 | A kind of optical system of multichannel fluorescence quantitative PCR instrument |
| CN108181239B (en) * | 2018-02-07 | 2023-09-12 | 张哲夫 | Optical system of multichannel fluorescence quantitative PCR instrument |
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| CN1235038C (en) | 2006-01-04 |
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