CN1546503A - Method for preparing polygonin and resveratrol - Google Patents
Method for preparing polygonin and resveratrol Download PDFInfo
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- CN1546503A CN1546503A CNA2003101125383A CN200310112538A CN1546503A CN 1546503 A CN1546503 A CN 1546503A CN A2003101125383 A CNA2003101125383 A CN A2003101125383A CN 200310112538 A CN200310112538 A CN 200310112538A CN 1546503 A CN1546503 A CN 1546503A
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- polygonin
- resveratrol
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- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Polymers C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 title claims abstract description 111
- 238000000034 method Methods 0.000 title claims abstract description 55
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 title claims abstract description 6
- 235000021283 resveratrol Nutrition 0.000 title claims abstract description 6
- 229940016667 resveratrol Drugs 0.000 title claims abstract description 6
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 52
- 238000000926 separation method Methods 0.000 claims abstract description 16
- 230000008569 process Effects 0.000 claims abstract description 12
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 48
- 235000018991 trans-resveratrol Nutrition 0.000 claims description 45
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 33
- 239000002904 solvent Substances 0.000 claims description 33
- 238000010828 elution Methods 0.000 claims description 28
- 239000000523 sample Substances 0.000 claims description 26
- 238000002360 preparation method Methods 0.000 claims description 24
- 150000001408 amides Chemical class 0.000 claims description 13
- 238000005516 engineering process Methods 0.000 claims description 10
- 239000004952 Polyamide Substances 0.000 claims description 9
- 229920002647 polyamide Polymers 0.000 claims description 9
- 230000008929 regeneration Effects 0.000 claims description 9
- 238000011069 regeneration method Methods 0.000 claims description 9
- 239000012488 sample solution Substances 0.000 claims description 8
- 238000001953 recrystallisation Methods 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 6
- 238000002425 crystallisation Methods 0.000 claims description 5
- 230000008025 crystallization Effects 0.000 claims description 5
- 238000000967 suction filtration Methods 0.000 claims description 5
- 238000004440 column chromatography Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 230000000750 progressive effect Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000011210 chromatographic step Methods 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 239000000284 extract Substances 0.000 abstract description 22
- 238000000605 extraction Methods 0.000 abstract description 14
- 241000196324 Embryophyta Species 0.000 abstract description 11
- 244000153955 Reynoutria sachalinensis Species 0.000 abstract description 6
- 235000003202 Reynoutria sachalinensis Nutrition 0.000 abstract description 6
- 240000006122 Chenopodium album Species 0.000 abstract 1
- 235000009344 Chenopodium album Nutrition 0.000 abstract 1
- 150000001298 alcohols Chemical class 0.000 abstract 1
- 229930182470 glycoside Natural products 0.000 abstract 1
- 150000002338 glycosides Chemical class 0.000 abstract 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 21
- 239000000047 product Substances 0.000 description 20
- 238000004519 manufacturing process Methods 0.000 description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 238000010898 silica gel chromatography Methods 0.000 description 10
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 9
- GNTDGMZSJNCJKK-UHFFFAOYSA-N divanadium pentaoxide Chemical compound O=[V](=O)O[V](=O)=O GNTDGMZSJNCJKK-UHFFFAOYSA-N 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 description 6
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
- 239000003960 organic solvent Substances 0.000 description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 5
- 230000002411 adverse Effects 0.000 description 5
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- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 238000009776 industrial production Methods 0.000 description 5
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- HSTZMXCBWJGKHG-CUYWLFDKSA-N trans-piceid Polymers O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=CC(\C=C\C=2C=CC(O)=CC=2)=C1 HSTZMXCBWJGKHG-CUYWLFDKSA-N 0.000 description 5
- HSTZMXCBWJGKHG-UHFFFAOYSA-N (E)-piceid Natural products OC1C(O)C(O)C(CO)OC1OC1=CC(O)=CC(C=CC=2C=CC(O)=CC=2)=C1 HSTZMXCBWJGKHG-UHFFFAOYSA-N 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000007795 chemical reaction product Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 230000006837 decompression Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000000945 filler Substances 0.000 description 4
- 229960003764 polydatin Drugs 0.000 description 4
- 239000000741 silica gel Substances 0.000 description 4
- 229910002027 silica gel Inorganic materials 0.000 description 4
- 229960001866 silicon dioxide Drugs 0.000 description 4
- 238000001291 vacuum drying Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 240000001341 Reynoutria japonica Species 0.000 description 3
- 240000004980 Rheum officinale Species 0.000 description 3
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- 239000003814 drug Substances 0.000 description 3
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- 239000002994 raw material Substances 0.000 description 3
- PJANXHGTPQOBST-VAWYXSNFSA-N trans-stilbene Chemical group C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 description 3
- 238000005160 1H NMR spectroscopy Methods 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 description 2
- 229910014033 C-OH Inorganic materials 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229910014570 C—OH Inorganic materials 0.000 description 2
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 2
- GOZCEKPKECLKNO-RKQHYHRCSA-N Picein Chemical compound C1=CC(C(=O)C)=CC=C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 GOZCEKPKECLKNO-RKQHYHRCSA-N 0.000 description 2
- 235000018167 Reynoutria japonica Nutrition 0.000 description 2
- 235000008081 Rheum officinale Nutrition 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 240000006365 Vitis vinifera Species 0.000 description 2
- 235000014787 Vitis vinifera Nutrition 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- OSASVXMJTNOKOY-UHFFFAOYSA-N chlorobutanol Chemical compound CC(C)(O)C(Cl)(Cl)Cl OSASVXMJTNOKOY-UHFFFAOYSA-N 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000003810 ethyl acetate extraction Methods 0.000 description 2
- -1 filter Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical compound C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 2
- 235000021286 stilbenes Nutrition 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000345998 Calamus manan Species 0.000 description 1
- 241001654861 Cissus assamica Species 0.000 description 1
- 241001289529 Fallopia multiflora Species 0.000 description 1
- 235000001018 Hibiscus sabdariffa Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000351396 Picea asperata Species 0.000 description 1
- 241000887162 Picea glehnii Species 0.000 description 1
- 241000510091 Quadrula quadrula Species 0.000 description 1
- 241000219061 Rheum Species 0.000 description 1
- 240000001745 Rheum palmatum Species 0.000 description 1
- 235000008090 Rheum palmatum Nutrition 0.000 description 1
- 235000005291 Rumex acetosa Nutrition 0.000 description 1
- 240000007001 Rumex acetosella Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- CSCPPACGZOOCGX-WFGJKAKNSA-N acetone d6 Chemical compound [2H]C([2H])([2H])C(=O)C([2H])([2H])[2H] CSCPPACGZOOCGX-WFGJKAKNSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 150000001555 benzenes Chemical group 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960004926 chlorobutanol Drugs 0.000 description 1
- SKCNIGRBPJIUBQ-UHFFFAOYSA-N chloroform;ethyl acetate Chemical compound ClC(Cl)Cl.CCOC(C)=O SKCNIGRBPJIUBQ-UHFFFAOYSA-N 0.000 description 1
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000004185 countercurrent chromatography Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002451 electron ionisation mass spectrometry Methods 0.000 description 1
- LJQKCYFTNDAAPC-UHFFFAOYSA-N ethanol;ethyl acetate Chemical compound CCO.CCOC(C)=O LJQKCYFTNDAAPC-UHFFFAOYSA-N 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 239000002024 ethyl acetate extract Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 235000002532 grape seed extract Nutrition 0.000 description 1
- 150000008282 halocarbons Chemical class 0.000 description 1
- 150000005826 halohydrocarbons Chemical class 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000012676 herbal extract Substances 0.000 description 1
- 238000010262 high-speed countercurrent chromatography Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- HZVOZRGWRWCICA-UHFFFAOYSA-N methanediyl Chemical group [CH2] HZVOZRGWRWCICA-UHFFFAOYSA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 238000005325 percolation Methods 0.000 description 1
- 235000020245 plant milk Nutrition 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
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- 238000003672 processing method Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 235000012950 rattan cane Nutrition 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- LUKBXSAWLPMMSZ-UHFFFAOYSA-N resveratrol Chemical group C1=CC(O)=CC=C1C=CC1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-UHFFFAOYSA-N 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 229920006395 saturated elastomer Chemical group 0.000 description 1
- 150000003333 secondary alcohols Chemical class 0.000 description 1
- 235000003513 sheep sorrel Nutrition 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- DKVBOUDTNWVDEP-NJCHZNEYSA-N teicoplanin aglycone Chemical compound N([C@H](C(N[C@@H](C1=CC(O)=CC(O)=C1C=1C(O)=CC=C2C=1)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)OC=1C=C3C=C(C=1O)OC1=CC=C(C=C1Cl)C[C@H](C(=O)N1)NC([C@H](N)C=4C=C(O5)C(O)=CC=4)=O)C(=O)[C@@H]2NC(=O)[C@@H]3NC(=O)[C@@H]1C1=CC5=CC(O)=C1 DKVBOUDTNWVDEP-NJCHZNEYSA-N 0.000 description 1
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Landscapes
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The invention relates to a method for preparing polygonin and resveratrol which comprises, using the conventional technological process for extraction from plants containing giant knotweed rhizome glycosides and / or chenopodium album alcohols and / or their extracts, separation by chromatography, and purifying.
Description
Technical field the present invention relates to separate in the material of a kind of self-contained polygonin and trans-resveratrol the method that prepare polygonin and trans-resveratrol, and particularly separation prepares the method for polygonin and trans-resveratrol from plant milk extract.Can realize the preparation of industrialization of polygonin and trans-resveratrol by the inventive method.
Background technology polygonin (/ glucoside) (having another name called polydatin polydatin, piceoside peicin).Chemical structure is 3,4 ', and 5-trihydroxy stilbene-3-β-list-D-glucoside (3,4 ', 5-trihydroxy-stilbene-3-β-D-glucoside).Its structure is as follows:
Trans-resveratrol (resveratrol), chemical structure are 3,4 ', the 5-trihydroxy stilbene, for trans diphenylethylene (be Stilbene, stilbene) compound.Its structure is as follows:
Polygonin and trans-resveratrol have various biologic activity, for example anti-oxidant, as to suppress tumour formation and development, microcirculation improvement and to the therapeutic action of shock etc.
See that the polygonin that is stated from document and the separation purification method of trans-resveratrol (for example mainly comprise solvent (as ethyl acetate, ether) extraction process, silica gel column chromatography etc., Yang Yun, Feng Weisheng, chemical composition of Chinese materia medica extraction separation handbook, Chinese medicine press, 1998:205-207; Sun Wenji, natural medicinal ingredients extraction separation and preparation, Chinese Medicine science and technology press, second edition, 1999:315-317).Some patent documentations relevant with the preparation of polygonin and aglycon thereof have also appearred in recent years.For example, " preparation method of trans-resveratrol and polidatin " (Chinese patent, application number 01118461.2), its method is that column chromatography and high speed adverse current chromatogram chromatography are combined, realize the first step of trans-resveratrol and polidatin separated promptly that (chromatographic flow is selected halohydrocarbon (as chloroform, methylene dichloride, tetracol phenixin) mutually for use by silica gel column chromatography, Fatty Alcohol(C12-C14 and C12-C18) or ketone (as methyl alcohol, ethanol, propyl alcohol, acetone)), again this first step separated product is undertaken separating the second time obtaining target product by the adverse current chromatogram chromatography.Another Chinese patent " trans-resveratrol and polidatin separation method and application thereof " (application number 00121100.5), its method relates to carries out ethyl acetate extraction to primary extract, and then adopt chloroform-methanol (or ethanol) or ethyl acetate-ethanol to carry out silica gel column chromatography as the chromatography elutriant, chloroform-ethyl acetate) and recrystallization (recrystallisation solvent: acetone-chloroform) obtain trans-resveratrol be that recrystallisation solvent carries out recrystallization and obtains polydatin with methyl alcohol-chloroform again, and by secondary chromatography (elutriant:.Obviously, this method also relates to a large amount of, the repeated use to organic solvent.The report that extracts trans-resveratrol and polydatin from giant knotweed also sees the clear 60-9455 of Japanese Patent (1985).This patented method is to adopt ether to obtain target product as extraction solvent, and its processing method is comparatively numerous and diverse, and the volatility of ether, inflammableness, toxicity are stronger than ethyl acetate, methyl alcohol equal solvent.
Above-mentioned these disclosed methods are suitable for a small amount of preparation under the laboratory scale more, and for the industrial production of mass-producing, its using value is limited.At first from separation method, aforesaid method all adopts silica gel column chromatography as essential separation means in various degree.There is the shortcoming that the sample separation amount is little, amount of filler is big in silicagel column, and filler silica gel is difficult to regeneration, so industrial scale is restricted, and the industrial production cost is higher.And the application of high-speed counter-current chromatography as described in Chinese patent 01118461.2, is the purity that silica gel column chromatography obtains to be higher than 70% and 90% product respectively carry out the second adverse current chromatographic separation, and whole technological process relates to multistep link, plurality of devices.In general, compare with silica gel column chromatography, the quantity of sample handling of countercurrent chromatography technology is littler, more difficultly satisfies the industrial production requirement.
In addition, from used solvent, above-mentioned document solvent for use system all uses one, two kind solvents such as halogenated hydrocarbon (chloroform etc.) or methyl alcohol etc. in various degree, guarantee that the production safety under the scale production condition is difficult relatively, and the solvent use cost is higher, and the finished product unavoidably can relate to harmful dissolvent residual problem.
Summary of the invention the invention provides a kind of from the plant that contains polygonin and trans-resveratrol or plant primary extract the method for separation and purification polygonin and trans-resveratrol.This method is easy and simple to handle, is easy to realize large-scale industrial production.
The present invention is characterized in the polymeric amide chromatography to be organically combined in the separation and purification preparation that is applied to polygonin and/or trans-resveratrol first.
The raw material that adopts the inventive method to extract purifying polygonin and trans-resveratrol comprises one or more a plant whole plant that contains polygonin and/or trans-resveratrol chemical ingredients or the part of plant, as root and/or stem and/or leaf and/or flower and/or fruit.For example giant knotweed (Polygonum cuspidatum), Tuber Fleeceflower Root (Polygonum multiflorum), whole plant or its certain a part of plant-origin of storehouse leaf dragon spruce (Picea glehnii), maple leaf rattan (Cissus assamica), grape (Vitis Vinifera), peanut (Arachis hypogaea), sorrel (Rheumpalmatum), Rheum tanguticum (Rheum tenguticum), Rheum officinale plants such as (Rheum officinale).
The raw material that adopts the inventive method to extract purifying polygonin and trans-resveratrol also can be above-mentioned plant or the former primary extract of its part base, these primary extracts comprise the extract of above-mentioned plant, for example water extract, alcohol extract, ethyl acetate extract etc. also can be extracts through extraction wait handle after the extract etc. of gained.
When former during as raw material with plant or its part base, then before adopting the polymeric amide chromatography, adopt moisture or water-containing organic solvent not, for example ethanol etc., extract at a certain temperature with routine techniques flow process known in the art, to obtain to contain the extract of polygonin and/or trans-resveratrol.These extracts can suitably be handled before carrying out the polymeric amide chromatography, for example, clarification, extraction, concentrate, drying etc.
The present invention's polymeric amide chromatography process comprises:
(1) primary extract that will contain polygonin and/or trans-resveratrol is gone up sample absorption: go up quadrat method and can adopt with suitable dissolution with solvents primary extract, these solvents comprise moisture or not aqueous methanol, ethanol, acetone or its similar solvent, and wherein preferred aqueous ethanol is as solvent; Should go up all product can be the solution that contains polygonin and/or resveratrol, also can be the solid sample of this solution after polymeric amide is mixed sample.The solution that wherein contains polygonin and/or resveratrol can be the extracting solution that contains polygonin and/or trans-resveratrol that adopts the routine techniques method to obtain from the plant material that contains polygonin and/or trans-resveratrol, also can be to extract solution gained solution after suitably pre-treatment (as clarification, concentrated, extraction etc.).
(2) chromatography wash-out: carry out the chromatography wash-out with the aqueous ethanol system as moving phase behind the sample on the extract.This elution system can be the aqueous ethanol of a certain specific concentrations, also can be the aqueous ethanolic solution of gradient concentration.Collect the wash-out effluent liquid, obtain containing polygonin and/or trans-resveratrol stream part.
(3) collect or collect respectively the wash-out stream part that contains polygonin and/or trans-resveratrol.
(4) handle the stream part contain polygonin and/or trans-resveratrol: the collected wash-out stream that contains polygonin and/or trans-resveratrol part, further adopt following one or more treatment process to handle: (1) will concentrated, the crystallization of collected stream part; Or (2) carry out the secondary chromatography with collected stream part; Or (3) adopt gac with collected stream part decolouring, concentrated, recrystallization.Obtain target component polygonin and/or trans-resveratrol thus.
Positively effect of the present invention is: the present invention is applied to polymeric amide chromatography technology the separation and purification of polygonin (and trans-resveratrol) first, by with the combination of target compound character, given full play to the peculiar advantage of this technology in the polygonin scale preparation.The outstanding feature of the inventive method is embodied in: polymeric amide chromatography post can regeneration, need not change filler and adorn post again between each batch produces; The control of commercial production conditions realizes that easily its required equipment is simple, easy and simple to handle, safety, and production cost is low, is easy to realize the conversion and the handing-over of the processing condition under the different industrial scales.On the basis of adopting the polymeric amide chromatography, can under the water-ethanol system, finish whole production processes, solvent systems is safer, significantly reduces solvent for use in the production process to producers and to the potential impact of environment, is more suitable for large-scale industrial production.
The following specific embodiment of embodiment is the description to process of the present invention, scope of the present invention is not constituted any restriction.
The new preparation process of embodiment 1.1 polygonins and trans-resveratrol
1. extract dry rhizome (prepared slices of Chinese crude drugs) 100g of plant polygonum cuspidatum, under 77-85 ℃ temperature,, extracted 2 hours at every turn with the 50% ethanol water refluxing extraction of 800ml 2 times.United extraction liquid, and concentrating under reduced pressure (60 ℃, 0.07-0.1Mpa) to cumulative volume 300ml, concentrated solution is transferred pH to 9-10 with the 2N sodium hydroxide solution, leaves standstill under the room temperature about 2 hours, and it is centrifugal that (4000r/min * 5min), remove residue, supernatant liquor is as last sample solution for standby.
2. separate and will go up on the sample solution sample (the about 500ml of column volume) to the standby polyamide column, the about 100ml/hr of flow velocity, last sample are adsorbed to article one colour band and move to sample on 1/2 place stops at the bottom of the nearly post.Successively with 300ml 20% ethanol water, 1000ml 60% ethanol water, 1000ml 90% ethanol water, normal pressure or pressurization chromatography gradient elution, the about 300ml/hr of elution speed.Collect 60%, 90% ethanol water elution part respectively, and be evaporated to original volume 1/20 (60 ℃, 0.07-0.1Mpa), suction filtration, respectively product I, product II.(suction filtration filtrate is incorporated next time into and is gone up in the sample solution).
The about 1.6g of product I, to add water to solution alcohol final concentration behind the 95-100% dissolve with ethanol is 30%, be splined on that (column volume 100ml) carries out the secondary chromatographic separation on the standby polyamide column, respectively with 100ml 25% ethanol water, 400ml 60% ethanol water gradient elution, the about 50ml/min of elution speed.Collect 60% ethanol water elution liquid, and concentrating under reduced pressure (55 ℃, 0.07-0.1Mpa) to 30ml, 4 ℃ of conditions leave standstill 1 hour crystallization, filter (40 ℃ of insolubles vacuum-dryings 12-72 hour, 0.08-0.1Mpa, the Vanadium Pentoxide in FLAKES siccative), get end product polygonin 1.2g.Contain polygonin 99.1% through the HPLC detection.
Product I (polygonin) detects:
1H-NMR (actone-d6) δ: 8.35 (2H, s, C3,4 '-OH), 7.43 (2H, d, C2 ', 6 '-H), 7.08 (1H, d, J=16, a-H), 6.89 (1H, d, J=16, β-H), 6.83 (2H, d, J=8, C3 ', 5 '-H), 6.76 (2H, d, C2 ', 6 '-H), 6.47 (1H, t, C4-H), 4.93 (1H, d, 1 "-H), 4.46-3.91 (4H, C2 ", 3 ", 4 "; 6 "-OH), 3.3-3.73 (6H, C2 ", 3 ", 4 " and, 5 ", 6 "-H).
The about 0.5g of product II, behind the 95-100% dissolve with ethanol, add water to solution and contain pure final concentration 50%, be splined on that (column volume 50ml) carries out the secondary chromatographic separation on the standby polyamide column, respectively with 100ml 60% ethanol water, 150ml 95% ethanol water gradient elution, the about 20ml/min of elution speed.Collect 95% ethanol water elution liquid, and concentrating under reduced pressure (50 ℃, 0.07-0.1Mpa) to 20ml, 4 ℃ of conditions leave standstill 1 hour crystallization, filter (40 ℃ of insolubles vacuum-dryings 24 hours, 0.08-0.1Mpa, the Vanadium Pentoxide in FLAKES siccative), get end product trans-resveratrol 0.2g.Contain trans-resveratrol 97.5% through the HPLC detection.
Product II (trans-resveratrol) detects: EI-MS (m/z): 228 (100%).
1HNMR(Acetone-d6)：8.45(1H，s，C4-OH)，8.18(2H，s，C3′，5′-OH)，7.40(2H，d，J＝8.7，C2，6-H)，7.00(1H，d，J＝16.2，a-H)，6.86(1H，d，J＝16.2，β-H)，6.81(2H，d，J＝8.7，C3，5-H)，6.52(2H，d，J＝2.1，C2′，6′-H)，6.25(1H，t，J＝2.1，C4′-H)。
Chromatography column is successively with after 90-95% ethanol water, the water elution regeneration, and is standby.
The new preparation process of embodiment 1.2 polygonins and trans-resveratrol
Among the embodiment, behind normal pressure or the pressurization chromatography gradient elution.Collect 60%, 90% ethanol water elution part respectively, and be evaporated to original volume 1/3 (60 ℃, 0.07-0.1Mpa), other steps are with embodiment 1.1.
The new preparation process of embodiment 1.3 polygonins and trans-resveratrol
Among the embodiment, behind normal pressure or the pressurization chromatography gradient elution.Collect 60%, 90% ethanol water elution part respectively, and be evaporated to original volume 1/50 (60 ℃, 0.07-0.1Mpa), other steps are with embodiment 1.1.
The preparation method of embodiment 2 polygonins
1. giant knotweed rhizome 100g suitably pulverizes, and extracts with the 800ml ethanol percolation, filter, concentrating under reduced pressure (50 ℃, 0.07-0.1Mpa) to final volume 50ml, adding 100ml water, and with 200ml ethyl acetate extraction 3 times, the extraction liquid concentrating under reduced pressure (50 ℃, 0.07-0.1Mpa) to 150ml, Silon is mixed sample, decompression volatilizes solvent, and is standby as last all product.
2. going up all product and use a dry method on a sample on standby polyamide column (the about 600ml of column volume), is that moving phase is carried out the chromatography wash-out with 1000ml water, 3000ml 30% ethanol water successively, flow velocity 600ml/hr.Collect 30% ethanol water elution effluent liquid, and will wherein contain polygonin stream and part merge, be evaporated to 100ml (60 ℃, 0.07-0.1Mpa), suction filtration, head product 2g, wherein polygonin content is 84%.
3. head product is with the 95-100% dissolve with ethanol, filter, filtrate adding water to contains pure final concentration 30%, adds 1% (ml/ml) medical active powdered carbon and boils 3 minutes, filtered while hot, filtrate decompression is concentrated into 50ml, leaves standstill 0.5 hour crystallization under 4 ℃, filters, (100 ℃ of gained crystal vacuum-dryings 4 hours, 0.08-0.1Mpa, the Vanadium Pentoxide in FLAKES siccative), get end product polygonin 1.5g.Contain polygonin 99.82% through the HPLC detection.
Embodiment 3.1 polygonin large-scale industrialization preparation methods
1. giant knotweed pharmaceutical decocting piece 500kg is through countercurrent extraction, concentrating under reduced pressure (60 ℃ 0.07-0.1Mpa) must be extracted concentrated solution 1200L, transfer concentrated solution pH to 10 with the 2N sodium hydroxide solution, left standstill under the room temperature about 2 hours, the centrifugal residue that goes, supernatant liquor is as last sample solution for standby.
2. with Silon (100-200 order),, adorn 2 posts altogether with routine techniques flow process dress post (the about 800L of column volume) known in the art, standby after installing.
3. go up sample solution and go up sample respectively and be adsorbed on 2 standby polyamide columns, the about 6L/hr. of last sample flow velocity, 4/5 place stops to go up sample at the bottom of last sample solution absorbs to article one colour band moves to nearly post.Successively with 800L 30% ethanol water, 2000L 60% ethanol water pressurization chromatography wash-out, wash-out pressure 10Bar., the 60% ethanol water elution part of collecting 2 chromatography columns is evaporated to (60 ℃ of 100L after the merging, 0.07-0.1Mpa), be 1/20 of original volume, room temperature leaves standstill 0.5 hour, and is centrifugal, obtain yellow-white powder 7300g, contain polygonin 82.2% through the HPLC detection.Standby as extracting solution or lower concentration elute soln respectively through the ethanolic soln that concentrating under reduced pressure reclaims.
4. chromatography column soaks with 5% sodium hydroxide solution respectively, changes solvent every day one time, and successive soaking is after 3 days, and to effluent liquid pH value 8～9, again with 10% acetic acid 2000L wash-out, to be washed to neutrality, chromatography column recovers stand-by state at last with water elution.
5. recrystallization purifying: above-mentioned chromatographic separation product 7300g, with 80L 95% dissolve with ethanol, filter, filtrate adding water to contains pure final concentration 30%, adds 0.3% (ml/ml) medical active powdered carbon and boils filtered while hot 3 minutes, filtrate decompression is concentrated into 50L, and room temperature leaves standstill 3 hours, and is centrifugal, get the near-white crystalline powder, decompression, and vacuum-drying 12 hours (60 ℃, 0.08-0.1Mpa, the Vanadium Pentoxide in FLAKES siccative), get end product polygonin 5500g.
The preparation method of embodiment 3.2 polygonins
Also can adopt in an embodiment and use 800L 30% ethanol water successively, 2000L 60% ethanol water normal pressure chromatography wash-out.Collect 60% ethanol water elution part of 2 chromatography columns, be evaporated to 1/5 of original volume after the merging, room temperature left standstill 0.5 hour, and other steps are with embodiment 3.1.
The preparation method of embodiment 3.3 polygonins
Collect 60% ethanol water elution part of 2 chromatography columns in an embodiment, be evaporated to 1/10 of original volume after the merging, also can pressurize the chromatography wash-out with 800L 30% ethanol water by ethanol water, 2000L 60% ethanol water pressurization chromatography wash-out, room temperature left standstill 4 hours.Other steps are with embodiment 3.1.
In specific implementation process, the also optional water of the aqueous ethanolic solution of progressive concentration gradient, 10～30% ethanol water, 30～60% ethanol water; 0～20% ethanol water, 20%～95% ethanol water; 30～60% ethanol water, 60～95% ethanol water.
Polygonin detects:
Content: this product detects through HPLC, contains polygonin 99.93%.
Residue on ignition: 0.1% (meeting relevant regulations under two appendix items of Chinese Pharmacopoeia version in 2000).
Heavy metal: meet relevant regulations under two appendix items of Chinese Pharmacopoeia version in 2000.
Dissolvent residual: (not using one, two kind solvents).
Limit test of microbe:, up to specification according to two appendix inspections of Chinese Pharmacopoeia version in 2000.
IR (KBr, cm
-1): 3373 (phenolic hydroxyl group υ
O-H), 3026 (phenyl ring υ
C-H), 1605,1591,1514,1448 (phenyl ring skeletal vibrations), 1341 (phenol-OH, υ
C-OH), 1263 (unsaturated ethers C-O-C, υ
C-O-C), 1172 (the C-H stretching vibration of methylene radical and saturated rings, υ
C-H), 1075 (stretching vibration of six-ring secondary alcohol, υ
C-OH), 1019,996,961 (the C-H flexural vibration on the phenyl ring, δ
C-H), 839 (C-H flexural vibration on the para-orientation phenyl ring, δ
C-H), 680 (C-H flexural vibration on the position substituted benzene ring, δ
C-H).
UV: the uv-absorbing of sample and parsing (as shown in table 1).
The uv-absorbing of table 1 sample and parsing
| Solvent | ??λ(nm) | Resolve |
| Methanol solution | ??217 | The E absorption band of fortified phenol |
| ??307，320 | The K absorption band that the phenyl ring of chromophore's replacement is arranged |
High resolution mass spectrum: measured value (M+H)
+=391.1378, theoretical value (M+H)
+=391.1387, the error between measured value and the theoretical value meets sample molecule formula: C within the measurement requirement scope
20H
22O
8Limit of error chemical formula C in the mass-spectrometric data storehouse
20H
23O
8Be (M+H)
+, with sample molecule formula C
20H
22O
8Conform to.
Embodiment discussion of results and analysis:
One. the foundation that chromatographic separating process is selected at first, from the character of target compound polygonin: it is soluble in methyl alcohol, ethanol, hot water, dissolves in ethyl acetate, sodium bicarbonate and aqueous sodium hydroxide solution, is dissolved in cold water slightly, is insoluble in ether.Secondly, from the needs of producing feasibility and cost keeping: production technique at first should possess feasibility, and operational path is simple more, simple, nothing is intersected, and the feasibility of its big production is big more; Next answers tool operability, security; Take into account simultaneously consumption less, principle that cost is low.
The present invention is applied to the separation and purification of polygonin (and trans-resveratrol) with polymeric amide chromatography technology, by with the combination of target compound character, given full play to the peculiar advantage of this technology in the polygonin scale preparation.
Two. foundation and purpose embodiment that the chromatographic separation technology is determined adopt clarification steps, last all product are carried out suitable pre-treatment, purpose is to reduce impurity to disturb, because giant knotweed herbal extract, not only contain effective ingredient, but also contain a large amount of invalid elements such as anthraquinone, tannin, polysaccharide and flavonoid composition etc., so before the employing column chromatography for separation, can be by the upper prop sample be carried out suitable pre-treatment, to simplify mask work, also can alleviate chromatography column simultaneously and pollute, improve the post utilization ratio.Last sample The pretreatment can also realize by the other technologies method, as to methods such as extracting solution extract.
In the methods of the invention, the primary extract that contains polygonin and trans-resveratrol can adopt sample on wet method or the dry method.Chromatography wash-out preferred alcohol-water is as eluting solvent, ethanol is three class organic solvents, its production cost is low, safe in utilization, and the chromatography wash-out adopts the alcohol-water of serial gradient concentration as the solvent gradient elution system, by 0～30% ethanol water → 30～60% ethanol water, reach active constituent-enriched (polygonin), remove impurity interferential purpose, chromatography column behind 30～60% ethanol water elutions, can continue wash-out with the ethanol water of greater concn, obtain containing the wash-out flow point of trans-resveratrol.The inventive method has realized on same chromatography column, carry out the purpose that wash-out makes effective constituent separation and enrichment by changing the solvent gradient system, and the used eluting solvent volume of chromatography is little, and the aftertreatment amount is little.Chromatography column has been finished the preliminary regeneration of chromatography column in the wash-out resveratrol components, carry out changing washing behind the suitable wash-out again, and chromatography column can reach stand-by state substantially.Technological process is simple to operate, economic security, product preparation amount are big.
Three, the outstanding feature of embodiment is embodied in:
1. solvent systems: the inventive method can be implemented in the extraction of finishing polygonin and trans-resveratrol under the water-ethanol system, separates, purifying.In disclosed polygonin and trans-resveratrol isolation technique, all relate to some extent and adopt eluting solvent such as one, two class organic solvents such as ether, chloroform, methyl alcohol and ethyl acetate etc. as extraction solvent or chromatography.Obviously, in experimental implementation and production preparation process, the solvent systems of the inventive method is safer, significantly reduces solvent for use in the production process to producers and to the potential impact of environment.
2. the production of industrialization, mass-producing: prepare in the document in separating of disclosed polygonin and trans-resveratrol, adopt methods such as silica gel column chromatography, organic solvent extraction, adverse current chromatogram chromatography.Obviously, in these methods, the amplification of its preparative-scale and preparation amount must be subjected to the restriction of self-technique condition.For example, when realizing the suitability for industrialized production of silica gel column chromatography, because (1) is too complicated and generally be difficult to regeneration as the regeneration step of the silica gel of chromatographic stuffing, and the requirement of the dress column condition of silicagel column and chromatography condition is very strict; (2) eluting solvent of silica gel column chromatography is generally the organic solvent that can not contain water, and as chloroform, ethyl acetate etc., these solvents are the cost height not only, its use and the condition handled also more strict; Therefore, the suitability for industrialized production of realization silica gel column chromatography certainly exists the restriction of technical difficulty and the rising significantly of production cost.And when adopting the ether equal solvent to extract, also there is similar problem in the use of solvent with handling.As for the high speed adverse current chromatogram chromatography, under the prior art condition, its every batch preparation amount generally can only be in milligram, is difficult to realize the suitability for industrialized production under these technical qualification.By contrast, in the methods of the invention, because (1) can finish whole production processes under the water-ethanol system; (2) polymeric amide chromatography post can regeneration, need not change filler and adorn post again between each batch produces; (3) control of commercial production conditions realizes easily, its required equipment is simple, easy and simple to handle, safety, production cost is low, be easy to realize the conversion and the handing-over of the processing condition under the different industrial scales, therefore in application facet, the outstanding advantage of the inventive method be art methods can not compare.
Claims (7)
1. the new preparation process of polygonin and trans-resveratrol is characterized in that polymeric amide chromatography technology is applied to the separation and purification of polygonin and/or trans-resveratrol.
2. method according to claim 1, its polymeric amide chromatography step comprises:
(1) last all product that will contain polygonin and/or trans-resveratrol are splined on chromatography column;
(2) ethanol that adopts concentration to increase progressively carries out gradient elution as eluting solvent;
(3) collect or collect respectively the wash-out stream part that contains polygonin and/or trans-resveratrol.
3. method according to claim 2 is characterized in that: going up all product can be the solution that contains polygonin and/or resveratrol, also can be the solid sample of this solution after polymeric amide is mixed sample.
4. method according to claim 2 is characterized in that: the collected wash-out stream that contains polygonin and/or trans-resveratrol part, and further adopt following one or more treatment process to handle: (1) will concentrated, the crystallization of collected stream part; Or (2) carry out the secondary chromatography with collected stream part; Or (3) adopt gac with collected stream part decolouring, concentrated, recrystallization.
5. preparation method according to claim 2 is characterized in that: the gradient concentration of indication (v/v) is followed successively by in the aqueous ethanolic solution of progressive concentration gradient: 0～80%, 20%～95%, 40%～100%.
6. preparation method according to claim 2 is characterized in that described polyamide column chromatography step comprises: Silon is with routine techniques flow process dress post known in the art; To go up on the sample solution sample to standby polyamide column, again successively with 0～30% ethanol water, 30～60% ethanol water normal pressures or pressurization chromatography gradient elution, collect 30～60% ethanol water elution parts, be evaporated to 1/3～1/50 of original volume, room temperature left standstill 0.5～4 hour, filtration or centrifugal, the insolubles drying under reduced pressure obtains yellow-white crystalline powder polygonin; Chromatography column regeneration back is standby.
7. according to claim 2 or 6 described preparation methods, it is characterized in that described polyamide column chromatography step comprises: will go up on the sample solution sample to standby polyamide column, when solution absorbs to article one colour band move to the nearly low side 1/2-4/5 of chromatography column place stop on sample; Successively with 0～30% ethanol water, 30～60% ethanol water, 60～95% ethanol water, normal pressure or pressurization chromatography wash-out, collect 30～60%, 60～95% ethanol water elution parts respectively, wherein 30～60% ethanol water elutions partly are evaporated to 1/3～1/50 of original volume, suction filtration or centrifugal gets the product polygonin, and 60～95% ethanol water elutions partly are evaporated to 1/3～1/50 of original volume, suction filtration or centrifugal gets the product trans-resveratrol; Chromatography column regeneration back is standby.
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| CN100338083C (en) * | 2005-09-13 | 2007-09-19 | 河北医科大学 | Method for preparing polygonin through tissue culture and inducement of hairy roots of giant knotweed |
| CN1935195B (en) * | 2005-09-21 | 2010-04-28 | 中国科学院大连化学物理研究所 | Method for extracting female sex hormone from polygonum cuspidatum |
| CN101338327B (en) * | 2008-08-13 | 2011-03-23 | 长沙华诚生物科技有限公司 | Process for extracting resveratrol with purity higher than 98 0.000000rom giant knotweed |
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