CN1546392A - Method for preparing composite silicate bacterium flocculant - Google Patents
Method for preparing composite silicate bacterium flocculant Download PDFInfo
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- CN1546392A CN1546392A CNA2003101111751A CN200310111175A CN1546392A CN 1546392 A CN1546392 A CN 1546392A CN A2003101111751 A CNA2003101111751 A CN A2003101111751A CN 200310111175 A CN200310111175 A CN 200310111175A CN 1546392 A CN1546392 A CN 1546392A
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- silicate bacteria
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- flocculation agent
- silicate
- composite flocculation
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Abstract
The invention discloses a process for preparing silicate bacteria composite flocculating agent which comprises, preparing silicate bacteria culture medium, preparing silicate bacteria flocculating agent, preparing active mineral material and preparing silicate bacteria composite flocculating agent. And the method of use comprises charging silicate bacteria composite flocculating agent into adsorption tank, mixing with the sewage water to be treated, agitating homogeneously, the contaminant being adsorbed by the flocculating agent and flowing into sedimentation tank for natural sedimentation, thus realizing solid-liquid separation.
Description
Technical field
The present invention relates to silicate bacteria, relate to composite flocculation agent simultaneously, specifically, relate to the method for making composite flocculation agent with silicate bacteria.
Background technology
Flocculation agent is a kind of material that is difficult for settled suspended particle flocking settling in the liquid that makes.The kind of flocculation agent comprises: inorganic flocculating agent, organic synthesis polymeric flocculant and biopolymer flocculation agent.Be the synthetic organic polymer flocculation agent of representative based on the inorganic flocculating agent of aluminium salt, molysite and multipolymer thereof with the poly amic acid in the flocculation agent, because of its good flocculating effect is widely used.But the inorganic flocculating agent consumption is generally bigger, and is easy to generate secondary pollution, and aluminium salt has toxicity, can influence human beings'health; Molysite can cause and have color in the treating water, and the iron of high density also can have a negative impact to human health and ecotope.The synthetic organic polymer flocculation agent not only costs an arm and a leg, and also can produce the material that has a strong impact on HUMAN HEALTH in production or degradation process, and its application is restricted.That the prior biological polymeric flocculant pollutes is little, cost is low, but consumption is big, a little less than the processing power, be mainly used in the secondary treatment of water treatment.Chinese patent application part 97106510.1 has been announced a kind of composite flocculation agent of being made up of natural organic matter and inorganics, and iron wherein, aluminum ion still have the anxiety of contaminate environment.
Chinese patent application part 01110311.5 discloses silicate bacteria, but up to now, still is difficult the technology appearance that biotechnology is made microbial cells with silicate bacteria and then made composite flocculation agent.
Summary of the invention
The purpose of this invention is to provide a kind of technology of the silicate bacteria enlarged culturing being made the microorganism composite flocculation agent with biotechnology.
Silicate bacteria is the common colloid bacillus of soil (Bacillus mucilaginosus), and this bacterial classification all has preservation in departments such as DSMZ of Institute of Microorganism, Academia Sinica and agricultural DSMZs of the Chinese Academy of Agricultural Sciences.
The method for making of silicate bacteria composite flocculation agent provided by the invention comprises:
(1) silicate bacteria medium preparation: by the about 1008 parts of preparation substratum of quality, wherein each component is: 5 parts of sucrose, magnesium sulfate heptahydrate (MgSO
47H
2O) 0.5 part, CaCO
30.1 part, Na
2HPO
42 parts, FeCl
30.005 part, 0.5 part of Erie's stone flour (also can select for use potassium felspar sand or other to contain the potassium breeze), above component thorough mixing evenly back add 1000 parts of clean waters, and mixing the back, to regulate pH value be 7.0～7.3 to be the liquid nutrient medium of cultivation silicate bacteria; If the ratio adding agar according to 2% in this substratum promptly can be made into solid medium, the ordinary method sterilising treatment is standby;
(2) preparation microbial floc: get activatory silicate bacteria bacterial classification (test tube kind), aseptic method is seeded in the silicate bacteria liquid nutrient medium and cultivates, postvaccinal substratum is cultivated 6 days (amount greatly then needs enlarged culturing step by step) under 28～30 ℃ condition, the bottle end, form stable white flocculent substance, this is the silicate bacteria flocculation agent, and above-mentioned a plurality of silicate bacteria cultures are merged, and filters, obtains filtrate, promptly make the silicate bacteria throw out, standby;
(3) preparation active mineral material: select for use mineral as raw material, be the active mineral material after the oven dry with adsorptivity;
(4) preparation of silicate bacteria composite flocculation agent: with silicate bacteria throw out and active mineral material mixing, fully the temperature with 60～70 ℃ is dried behind the mixing, is crushed to 100 orders then and sieves, and promptly makes silicate bacteria composite flocculation agent finished product.
This programme is selected the mineral with adsorptivity for use, as: one or more in zeolite, sepiolite, montmorillonite, the diatomite etc.
The using method of silicate bacteria composite flocculation agent of the present invention is:
(1) in the sewage introducing flocculation adsorption tank that will need to handle, add the silicate bacteria composite flocculation agent, stir, pollutent is wherein adsorbed by flocculation agent, through after the absorption reaction, a large amount of pollution substances are flowed into the settling tank natural subsidence by the part of the flocs unit of flocculation agent absorption becoming mud flco, realize solid-liquid separation;
(2) clear water is discharged after the solid-liquid separation, mud then enters the sludge activation pond, with the biofloculation adsorption function of precipitating sludge short period of time aeration with recovery mud, the partial organic substances of can also degrading simultaneously, then active sludge is sent into again in the flocculation adsorption tank, be used for disposing of sewage with the silicate bacteria composite flocculation agent.
Silicate bacteria composite flocculation agent of the present invention has following advantage compared with similar products: (1) high efficiency.Under the equal consumption, its processing efficiency is apparently higher than traditional flocculant; (2) safety non-toxic.Prove the silicate bacteria safety non-toxic through the small white mouse proof test.The flocculation agent of being made by silicate bacteria can be used for the fermentation aftertreatment of industries such as food, medicine fully; (3) non-secondary pollution.Mostly be polymer substances such as glycoprotein, mucopolysaccharide, Mierocrystalline cellulose, DNA by the agglutinating matter that this silicate bacteria produced, its molecular weight is many 10
5More than, have bio-degradable, thereby can not bring secondary pollution; (4) of many uses, decolorizing effect is remarkable.This microbial flocculant is based on silicate bacteria, at Ca
2+Under metal ion and other batching fellowship, intestinal bacteria, yeast, slime water, livestock products waste water, waste water from dyestuff etc. there are fabulous flocculation and decolorizing effect; (5) injected volume is few relatively.Use a small amount of this microbial flocculant, just can realize the cleaning action of big area sewage; (6) the strain culturing nutritional requirement is simple, and growth cycle is short, and production cost is low.
This silicate bacteria composite flocculation agent application aspect water treatment:
(1) high concentrated organic wastewater is handled
Livestock products waste water is the higher unmanageable class waste water of BOD value, adopts synthetic polymeric flocculant to handle and has secondary pollution, and add Ca with the silicate bacteria composite flocculation agent
2+After handling 10min, supernatant liquor is approaching transparent, TOC (total organic carbon), OD
660Value all has remarkable reduction, and turbidity removal rate reaches 94.5%.Add the silicate bacteria composite flocculation agent in the tanning trade effluent, its turbidity removal rate can reach 96%.
(2) decolouring of waste water from dyestuff
Because waste water from dyestuff is difficult to biological degradation, and general chemical floc treatment effect is not good, at this moment uses the silicate bacteria composite flocculation agent to handle and to reach the ideal effect.To the processing of decolouring of the darker waste water of colors such as black liquor, its percent of decolourization can reach more than 95% and 98% respectively this silicate bacteria composite flocculation agent in the laboratory.
(3) high density inorganics suspension wastewater treatment
The silicate bacteria composite flocculation agent can be used for the processing of water samples such as kaolin, muddy water slurry, flyash.When handling among the 100ml loess muddy water up to 15g, be 3 times of anionic polyacrylamide flocculation agent with the silicate bacteria flocculation agent.
(4) processing of expansion active sludge
Many trade effluents are in adopting the Wastewater Treated by Activated Sludge Process process, and the active sludge of formation expands easily, thereby influences processing efficiency, if in active sludge, add microbial flocculant, sludge volume index can very fast decline, thereby eliminate the sludge bulking state, recovers the sedimentation capacity of active sludge.As the expansion active sludge that forms in the pharmacy waste water biochemical treatment process, add the silicate bacteria composite flocculation agent when therein, sludge settling property SVI is very fast to drop to 50mL/g from 260mL/g, has eliminated sludge bulking, has recovered the sedimentation capacity of active sludge.
(5) purifying treatment of wastewater from chemical industry
The silicate bacteria composite flocculation agent has good flocculating effect to black liquid, leather waste water, azo dye wastewater, thioxine dyes waste water, electroplating wastewater, color printing making sheet waste water, petrochemical wastewater, coinage waste water and blue ink, burnt black ink etc., test shows, waste water solid is separated obviously, CODcr clearance 55%～98%, and suspended substance, colourity, turbidity removal rate are more than 90%.
Embodiment
Embodiment: at first, preparation silicate bacteria substratum: get sucrose 5.0g, MgSO
47H
2O 0.5g, CaCO
30.1g, Na
2HPO
42.0g, FeCl
30.005g Erie's stone flour (also can select for use potassium felspar sand or other to contain the potassium breeze) 0.5g mixes the back and adds distilled water 1000ml, mixes, regulating the pH value is 7.0～7.3; In this base-material, add 20g agar and promptly get the solid medium of cultivating silicate bacteria, standby; The ordinary method sterilising treatment.
Second, preparation microbial flocculant: get activatory silicate bacteria bacterial classification (test tube kind), aseptic method is seeded in the silicate bacteria liquid nutrient medium and cultivates, postvaccinal substratum is cultivated 6 days (amount greatly then needs enlarged culturing step by step) under 28～30 ℃ condition, the bottle end, form stable white flocculent substance, and this is the silicate bacteria flocculation agent, above-mentioned a plurality of silicate bacteria cultures are merged, filter, obtain filtrate, promptly make the silicate bacteria flocculation agent, standby;
The 3rd, preparation active mineral material: with the zeolite is raw material, is the active mineral material after the oven dry;
The 4th, synthetic silicate bacterium composite flocculation agent: with silicate bacteria culture (after the filtered water) and the 10g active mineral material mixing of 1000ml, fully the temperature with 60～70 ℃ is dried behind the mixing, is crushed to 100 orders and sieves, and promptly makes about 12g silicate bacteria composite flocculation agent finished product.
Claims (2)
- The preparation method of 1 silicate bacteria composite flocculation agent, its feature comprises:(1) silicate bacteria medium preparation: by the about 1008 parts of preparation substratum of quality, wherein each component is: 5 parts of sucrose, 0.5 part of magnesium sulfate heptahydrate, CaCO 30.1 part, Na 2HPO 42 parts, FeCl 30.005 part, 0.5 part of Erie's stone flour or potassium felspar sand, above component thorough mixing evenly back add 1000 parts of clean waters, and mixing the back, to regulate pH value be 7.0～7.3 to be the liquid nutrient medium of cultivation silicate bacteria; Ratio adding agar according to 2% in this base-material promptly can be made into solid medium, and the ordinary method sterilising treatment is standby;(2) preparation microbial floc: get activatory silicate bacteria bacterial classification, aseptic method is seeded in the silicate bacteria liquid nutrient medium and cultivates, postvaccinal substratum was cultivated 6 days under 28～30 ℃ condition, the bottle end, form stable white flocculent substance, this is the silicate bacteria flocculation agent, and above-mentioned a plurality of silicate bacteria cultures are merged, and filters, obtains filtrate, promptly make the silicate bacteria throw out, standby;(3) preparation active mineral material: choose mineral, be the active mineral material after the oven dry with adsorptivity;(4) preparation of silicate bacteria composite flocculation agent: with silicate bacteria throw out and active mineral material mixing, fully the temperature with 60～70 ℃ is dried behind the mixing, is crushed to 100 orders then and sieves, and promptly makes silicate bacteria composite flocculation agent finished product.
- The preparation method of 2 silicate bacteria composite flocculation agents as claimed in claim 1 is characterized in that selected mineral with adsorptivity, can be wherein one or more of zeolite, sepiolite, montmorillonite, diatomite.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310111175 CN1262485C (en) | 2003-12-08 | 2003-12-08 | Method for preparing composite silicate bacterium flocculant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310111175 CN1262485C (en) | 2003-12-08 | 2003-12-08 | Method for preparing composite silicate bacterium flocculant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1546392A true CN1546392A (en) | 2004-11-17 |
| CN1262485C CN1262485C (en) | 2006-07-05 |
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ID=34335970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200310111175 Expired - Fee Related CN1262485C (en) | 2003-12-08 | 2003-12-08 | Method for preparing composite silicate bacterium flocculant |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101693561B (en) * | 2009-10-13 | 2011-04-20 | 河海大学 | Method for preparing compound flocculating agent |
| CN102531140A (en) * | 2012-01-19 | 2012-07-04 | 中国科学院地球化学研究所 | Method for using Bacillus mucitaginosus to treat mercury-containing waste water |
| CN104109641A (en) * | 2013-04-19 | 2014-10-22 | 中国地质科学院郑州矿产综合利用研究所 | Method for rapidly screening silicate bacteria |
| CN107473318A (en) * | 2017-08-22 | 2017-12-15 | 承德人和矿业有限责任公司 | One kind contains illitic mineral complex and preparation method and purposes |
| CN111194589A (en) * | 2020-01-14 | 2020-05-26 | 梁钧 | Desert algae mixed sand fixation method |
| CN114177887A (en) * | 2021-12-02 | 2022-03-15 | 中国环境科学研究院 | Biochar-manganese composite material wrapping biochar and preparation method and application thereof |
-
2003
- 2003-12-08 CN CN 200310111175 patent/CN1262485C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101693561B (en) * | 2009-10-13 | 2011-04-20 | 河海大学 | Method for preparing compound flocculating agent |
| CN102531140A (en) * | 2012-01-19 | 2012-07-04 | 中国科学院地球化学研究所 | Method for using Bacillus mucitaginosus to treat mercury-containing waste water |
| CN102531140B (en) * | 2012-01-19 | 2014-04-02 | 中国科学院地球化学研究所 | Method for using Bacillus mucitaginosus to treat mercury-containing waste water |
| CN104109641A (en) * | 2013-04-19 | 2014-10-22 | 中国地质科学院郑州矿产综合利用研究所 | Method for rapidly screening silicate bacteria |
| CN107473318A (en) * | 2017-08-22 | 2017-12-15 | 承德人和矿业有限责任公司 | One kind contains illitic mineral complex and preparation method and purposes |
| CN111194589A (en) * | 2020-01-14 | 2020-05-26 | 梁钧 | Desert algae mixed sand fixation method |
| CN114177887A (en) * | 2021-12-02 | 2022-03-15 | 中国环境科学研究院 | Biochar-manganese composite material wrapping biochar and preparation method and application thereof |
| CN114177887B (en) * | 2021-12-02 | 2023-01-03 | 中国环境科学研究院 | Biochar-manganese composite material wrapping biochar and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1262485C (en) | 2006-07-05 |
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